Digital Commons Network^™

Hydrotic Ectodermal Dysplasia(Associated With Numerous Enclusedpermanent Teeth Including A Primary Molar), Ali Rıza Alpöz

Turkish Journal of Medical Sciences

No abstract provided.

Opinions Of Assistant Doctors In Süleymandemirel University Medical Faculty Aboutstatistical Knowledge, Mustafa Öztürk, Veli̇ddi̇n Selçuk Engi̇n, Ahmet Nesi̇mi̇ Ki̇şi̇oğlu

Turkish Journal of Medical Sciences

In scientific investigations; statistical knowledge is essential for collection and analysis of data properly as well as correct interpretation of literature information. In this study that was made in March 1997, opinions of 46 assistant doctors in Süleyman Demirel University Medicine Faculty about statistical knowledge were investigated by a questionnaire. All respondents have agreed on the fact that comprehension of the statistical process in an article was necessary for throughout understanding. Besides, 43 of them (93.5%) admitted that their present statistical knowledge was few or absent at all. All assistant doctors stated that a statistical education program need to be …

Marfan Syndrome, Behzat Özkan, Naci̇ Cevi̇z, Mustafa Büyükavci

Turkish Journal of Medical Sciences

No abstract provided.

Quantification Of Total Collagen In Rabbit Tendonby The Sirius Red Method, Dilek Taşkiran, Emin Taşkiran, Hüseyin Yercan, Fatma Z. Kutay

Turkish Journal of Medical Sciences

No abstract provided.

A Patient With Three Simultaneously Occurring Malignancies, Bahar Kiliçarslan, Gülay Özbi̇li̇m, Gökbay Elpek, Şeyda Karaveli̇, Mi̇ne Üner

Turkish Journal of Medical Sciences

No abstract provided.

Molecular Studies Of Splice Sites In The Canine Dystrophin Gene, Hayley Durling

Theses : Honours

The development of an effective therapy for Duchenne Muscular Dystrophy (DMD) is one of the primary goals of all DMD/Becker Muscular Dystrophy (BMD) research. Golden Retriever Muscular Dystrophy (GRMD), an animal model of DMD is a fatal degenerative myopathy. Unlike the mdx model, the GRMD dog more accurately reflects the phenotype shown by human DMG patients, making the model better suited for the investigation and assessment of potential therapeutic approaches. The GRMD mutation, a base change from A to G in the 3' splice acceptor site of intron 6, results in exon 7 skipping which disrupts the translational reading frame. …

The Role Of Gap Junctions In Congenital Diseases Of The Heart, Scott Henry Britz-Cunningham

Loma Linda University Electronic Theses, Dissertations & Projects

Background. Gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. We hypothesized that mutations altering sites critical to this regulation would lead to functional or developmental abnormalities of the heart.

Methods. Connexin43 DNA from 25 normal subjects and 30 children with a variety of congenital heart diseases was amplified by the polymerase chain reaction and sequenced. Mutant DNA was expressed in cell culture and examined for its effect …

Influence Of Physical Setting On Seagrass Landscapes Near Beaufort, North Carolina, Usa, Mark S. Fonseca, Susan S. Bell

Integrative Biology Faculty and Staff Publications

Field surveys were conducted in Core and Back Sounds, North Carolina, USA, to relate the physical setting of seagrass beds, as measured by a wave exposure index (REI), tidal current speed and water depth, to various measures of the sedimentary environment, spatial heterogeneity of seagrass distribution and measures of seagrass abundance. Seagrass beds in this area form patterns ranging from continuous to semi-continuous to widely dispersed, discrete patches across a gradient of increasing hydrodynamic activity. Tidal current speeds, exposure to waves and relative water depths revealed strong correlative evidence that physical processes influenced landscape-scale (50 x 50 m range with …

Update - July 1998, Loma Linda University Center For Christian Bioethics

Update

In this issue:

-- Christianity and Human Cloning - A Seventh-day Adventist Declaration of Ethical Principles
-- Cloning: Dolly's Mother is Her Sister!?
-- Hello, Dolly! Dolly! Dolly!

Expression Of Constitutively Active Raf-1 In The Mitochondria Restores Antiapoptotic And Leukemogenic Potential Of A Transformation-Deficient Bcr/Abl Mutant., P Salomoni, M A Wasik, R F Riedel, K Reiss, J K Choi, T Skorski, B Calabretta

Department of Microbiology and Immunology Faculty Papers

The oncogenic BCR/ABL protein protects hematopoietic cells from apoptosis induced by growth factor deprivation, but the mechanisms are only partially understood. A BCR/ABL mutant lacking amino acids 176-426 in the BCR domain (p185DeltaBCR) failed to protect interleukin 3-deprived 32Dcl3 myeloid precursor cells from apoptosis, although it possessed tyrosine kinase activity and was capable of activating the Ras-Raf-MAP kinase pathway. Compared to p185 wild-type transfectants, p185DeltaBCR-transfected cells showed markedly reduced levels of Bcl-2 and expressed the hypophosphorylated, proapoptotic form of BAD. Bcl-2 expression in the mitochondrial fraction of p185DeltaBCR cells was also markedly diminished and mitochondrial RAF was undetectable. In p185DeltaBCR …

Knowledge, Attitudes And Behavior Of African American Undergraduate College Students Concerning Primary Preventative Health, Lionas Mayes

McCabe Thesis Collection

African Americans' knowledge about primary preventive health and their attitudes toward primary preventive health influence their health behavior. Primary preventive health behavior is also affected by the individuals' definition of health and the significance they ascribe to their health status. Attitudes toward primary preventive health determine whether health assessment is important in preventing disease. African Americans have a myriad of views about health and general well being. The purpose of this study is to determine the relationship between primary preventive health knowledge, primary preventive health attitudes and primary preventive health behavior in African American undergraduate students. A survey will be …

Enhancement Of Atp Levels And Glucose Metabolism During An Infection By Chlamydia: Nmr Studies Of Living Cells, David M. Ojcius, H. Degani, Joel Mispelter, Alice Dautry-Varsat

All Dugoni School of Dentistry Faculty Articles

The Chlamydia species are obligate intracellular bacteria that proliferate only within the infected cell. Since the extracellular bacteria are metabolically inert and there are no cell-free systems for characterizingChlamydia metabolism, we studied metabolic changes related to ATP synthesis and glycolysis in HeLa cells infected withChlamydia psittaci during the course of the 2-day infection cycle using noninvasive 31P and 13C NMR methods. We find that the infection stimulates ATP synthesis in the infected cell, with a peak of ATP levels occurring midway through the infection cycle, when most of the metabolically active bacteria are proliferating. The infection also stimulates synthesis of …

The Effect Of Tape And Neoprene Ankle Supports On Balance In Athletes With Injured Ankles, Tara Michelle Gerlach

Theses, Dissertations and Capstones

Injuries to the ankle joint are among the most common in athletics (Feuerbach et al., 1994; Gross, 1987). As clinicians, it is important to investigate various factors which could potentially reduce the occurrence of ankle injuries. One such actors is an improvement in proprioception (Cox et al., 1993; Glencross & Thornton, 1994). A suggested method for improving proprioception in the ankle is application of an external support, such as tape or a brace (Feuerbach et al. , 1994; Karlsson & Andreasson, 1992 ) . This study compared the proprioceptive abilities of the ankle under three conditions: unsupported, taped, and using …

Characterization Of The Promoter And Upstream Activation Sequences Of Sporulation-Specific Genes Spr1 (Ssg1) And Spr2 In Saccharomyces Cerevisiae, Sharmini S. Soosaithasan

Theses, Dissertations and Capstones

Sporulation is a developmental process in which temporally distinct sets of genes regulate the processes of meiosis and ascospore formation. Sporulation-specific genes are classified into early, middle, and late genes based on their peak time of expression during sporulation. Regulation of two late genes, SPR1 and SPR2, was studied. The function of SPR2 is unknown. Its regulatory region contains two Mid Sporulation Elements (MSE) repeated in tandem that are sufficient to confer sporulation-specific regulation to a CYCl-lacZ reporter. These sequences have UAS (upstream activation sequence) properties, as each 14 base pair element is sufficient to confer sporulation-specific regulation …

Molecular Determinant Of Ion Selectivity Of A (Na+ + K+)-Coupled Rat Brain Glutamate Transporter, Yumin Zhang, Annie Bendahan, Ruth Zarbiv, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters remove this neurotransmitter from the synaptic cleft by a two-stage electrogenic process, in which glutamate is first cotransported with three sodium ions and a proton. Subsequently, the cycle is completed by translocation of a potassium ion in the opposite direction. Recently, we have identified an amino acid residue of the glutamate transporter GLT-1 (Glu-404) that influences potassium coupling. We have now analyzed the effect of seven other amino acid residues in the highly conserved region surrounding this site. One of these residues, Tyr-403, also proved important for potassium coupling, because mutation to Phe (Y403F) resulted in an electroneutral …

Cysteine Scanning Of The Surroundings Of An Alkali-Ion Binding Site Of The Glutamate Transporter Glt-1 Reveals A Conformationally Sensitive Residue, Ruth Zarbiv, Myriam Grunewald, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters remove this transmitter from the extracellular space by cotransport with three sodium ions and a proton. The cycle is completed by translocation of a potassium ion in the opposite direction. Recently we have identified two adjacent amino acid residues of the glutamate transporter GLT-1 that influence potassium coupling. Using the scanning cysteine accessibility method we have now explored the highly conserved region surrounding them. Replacement of each of the five consecutive residues 396–400 by cysteine abolished transport activity but at several other positions the substitution is tolerated. One residue, tyrosine 403, was identified where cysteine substitution renders the …

Arachidonic Acid Activates A Proton Current In The Rat Glutamate Transporter Eaat4, Anastassios V. Tzingounis, Chien-Liang Lin, Jeffrey D. Rothstein, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The excitatory amino acid transporter EAAT4 is expressed predominantly in Purkinje neurons in the rat cerebellum (1-3), and it participates in postsynaptic reuptake of glutamate released at the climbing fiber synapse (4). Transporter-mediated currents in Purkinje neurons are increased more than 3-fold by arachidonic acid, a second messenger that is liberated following depolarization-induced Ca²⁺ activation of phospholipase A₂ (5). In this study we demonstrate that application of arachidonic acid to oocytes expressing rat EAAT4 increased glutamate-induced currents to a similar extent. However, arachidonic acid did not cause an increase in the rate …

Macroscopic And Microscopic Properties Of A Cloned Glutamate Transporter/Chloride Channel, Jacques I. Wadiche, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The behavior of a Cl⁻ channel associated with a glutamate transporter was studied using intracellular and patch recording techniques in Xenopus oocytes injected with human EAAT1 cRNA. Channels could be activated by application of glutamate to either face of excised membrane patches. The channel exhibited strong selectivity for amphipathic anions and had a minimum pore diameter of ∼5Å. Glutamate flux exhibited a much greater temperature dependence than Cl⁻ flux. Stationary and nonstationary noise analysis was consistent with a sub-femtosiemen Cl⁻ conductance and a maximum channelP_o ≪ 1. The glutamate binding rate was similar to estimates …

Monitoring Of Laboratory Parameters Duringvalproic Acidcarbamazepine Combinationtherapy, F. Müjgan Aynaci, Hilal Mocan, Osman Aynaci, Yakup Aslan

Turkish Journal of Medical Sciences

No abstract provided.

Harnessing The Human Genome Through Legislative Restraint, George P. Smith Ii

Scholarly Articles

The awesome predictive power of genetic medicine promises great advancements in not only the treatment of identifiable conditions but the prevention of their pathological manifestations. At the same time, the release and dissemination of this genetic or medical information poses a distinct risk of loss of privacy and stigmatization to carriers of genetic disorders. In order to safeguard the individual right of autonomy, privacy, confidentiality and informed consent-yet accommodate the legitimate interests of employers and insurers to obtain medical information relevant to their professional needs and economic responsibilities a balance must be struck legislatively at the federal and state levels …

In Vivo And In Vitro Nuclear Magnetic Resonance Imaging And Spectroscopy Of Psychoactive Drugs In Rat Brain, Diana M. Lindquist

Theses and Dissertations

Four psychoactive drugs were studied using various in vivo nuclear magnetic resonance (NMR) techniques. Trifluoperazine (TFP) was given to several normal rats, whose brains were then studied using in vivo fluorine-19 NMR. Three peaks were detected in the in vivo fluorine spectra: one at -62 ppm, which was due to TFP, and two others at -55 and -75 ppm, which are due to unknown metabolites of TFP. Samples of skin, skull, muscle, and brain were obtained from these rats and extracted for in vitro analysis. The metabolites responsible for the in vivo peak at -75 ppm also appeared in the …

Tracheopathia Osteoplastica, Adil Zamani, Ayşe Gül Zamani, Ergün Bikmaz, Oktay İmeci̇k

Turkish Journal of Medical Sciences

No abstract provided.

Tubo Ovarian Abscess: Risk Factors And Clinicalfeatures In Turkish Population, Didem Akyol, Utku Özcan, Murat Eki̇n, Tayfun Güngör, Oya Gökmen

Turkish Journal of Medical Sciences

No abstract provided.

Digital Amputations Caused By Amniotic Bandsa Case Report, Volkan Baltaci, Didem Akyol, Davut Gül, Bekir Sıtkı Sayli

Turkish Journal of Medical Sciences

No abstract provided.

Ischemia-Reperfusion Injury, Reactive Oxygenmetabolites, And The Surgeon, A.Özdemir Aktan, A. Süha Yalçin

Turkish Journal of Medical Sciences

No abstract provided.

Blood Lactate/Pyruvate As A Predictor Of Shockdevelopment In Acute Myocardial Infarction, Arzu Seven, Sabiha Ci̇velek, Figen Kocaman, Lale Koldaş, Gülden Candan

Turkish Journal of Medical Sciences

This study, designed to investigate the blood lactate and pyruvate levels in AMI, was carried out on 41 patients. Based upon the localization of AMI, the patients were studied under anterior AMI (n=17), anteroseptal AMI (n=9) and inferior AMI (n=15) groups. Left ventricular failure developed in five patients with anterior AMI and in one patient with anteroseptal AMI. Cardiogenic shock developed in two patients with anterior AMI. Lactate and pyruvate were analysed in the blood samples drawn 24-48 hours after AMI. CPK, CPK-MB, AST and LDH activities were determined in the sera of blood samples drawn within 6-24 hours of …

Molecular Analysis Of The Factor V-Leiden Mutation In A Cardiac Transplant Patient Population, Caroline Maher

Theses

Venous thrombosis is a serious health problem affecting 1 in 1000 individuals annually. Until recently the pathogenic factors underlying thrombosis were associated with genetic defects in protein C, protein S and antithrombin III. However these were recognised in fewer than 5-10% of thrombotic patients. A breakthrough was made with the discovery of activated protein C resistance (APCr) which is associated in 90% of cases v/herein a G-->A transition in the factor V gene results in an abnormal molecule, Factor V Leiden. APCr is a major risk factor for thrombosis being present in 20-60% of thrombotic patients. Recently a G-^A …

Factor Vii Deficiency Presenting Withmenometrorrhagia, Gürden Gür, Mete Özdoğan, Orhan Özgür, Sedat Boyacioğlu, Hasan Telatar

Turkish Journal of Medical Sciences

No abstract provided.

The Role Of Ultrafiltration Therapy On Bloodpressure Of Hypertensive Chronic Hemodialysischildren, Caner Kabasakal, Sevgi Mi̇r, Ferah Sönmez, Nermin Beti̇n, Alpan Cura, Ruhi Özyürek

Turkish Journal of Medical Sciences

Hypervolemia is supposed to be the principal factor in hypertension in chronic hemodialysis adults. In this study, the role of intensive ultrafiltration (UF) treatment to reduce hypertension due to hypervolemia in hemodialysis children was investigated. The inferior vena cava (IVC) was also evaluated as an index of UF therapy. Twenty-three hemodialysis children with the mean age 10.6±3.1 years were classified due to their blood pressure status. IVC measurements were performed with 2-D echocardiography and inferior vena cava index (IVCI) was calculated as diameter of inferior vena cava (mm)/ body surface area (m 2 ). UF therapy was applied to each …

A Systematic Evaluation Of Methods To Separate X- And Y- Bearing Sperm, Jacquelyn Irving

Theses: Doctorates and Masters

This project was initiated to determine if was possible to enrich either X- or Y- bearing sperm, and hence to preselect the sex of a child. Two of the possible reasons why couple might want to select the sex of a child are firstly because of a family history of an X-linked recessive genetic disorder, which usually only affect sons, and secondly families who have had several children of one sex. For this study, men with three or more children of the same sex were recruited following the publication of an article in The West Australian newspaper. The percentage of …