Digital Commons Network^™

Reduced Hamster Usage And Stress In Propagating Leishmania Chagasi Promastigotes Using Cryopreservation And Saphenous Vein Inoculation, Soi Meng Lei, Amanda E. Ramer-Tait, Rebecca R. Dahlin-Laborde, Kathleen Mullin, Jeffrey K. Beetham

Amanda Ramer-Tait Publications

Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation …

Individuality In Gut Microbiota Composition Is A Complex Polygenic Trait Shaped By Multiple Environmental And Host Genetic Factors, Andrew K. Benson, Scott A. Kelly, Ryan Legge, Fangrui Ma, Soo Jen Low, Jaehyoung Kim, Min Zhang, Phaik Lyn Oh, Derrick Nehrenberg, Kunjie Huab, Stephen D. Kachman, Etsuko N. Moriyama, Jens Walter, Daniel A. Peterson, Daniel Pomp

Center for Plant Science Innovation: Faculty Publications

In vertebrates, including humans, individuals harbor gut microbial communities whose species composition and relative proportions of dominant microbial groups are tremendously varied. Although external and stochastic factors clearly contribute to the individuality of the microbiota, the fundamental principles dictating how environmental factors and host genetic factors combine to shape this complex ecosystem are largely unknown and require systematic study. Here we examined factors that affect microbiota composition in a large (n = 645) mouse advanced intercross line originating from a cross between C57BL/6J and an ICR-derived outbred line (HR). Quantitative pyrosequencing of the microbiota defined a core measurable microbiota …

Role Of Transforming Growth Factor Beta In Corneal Function, Biology And Pathology, Ashish Tandon, Jonathan C. K. Tovey, Ajay Sharma, Rangan Gupta, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Transforming growth factor-beta (TGFβ) is a pleiotropic multifunctional cytokine that regulates several essential cellular processes in many parts of the body including the cornea. Three isoforms of TGFβ are known in mammals and the human cornea expresses all of them. TGFβ1 has been shown to play a central role in scar formation in adult corneas whereas TGFβ2 and TGFβ3 have been implicated to play a critical role in corneal development and scarless wound healing during embryogenesis. The biological effects of TGFβ in the cornea have been shown to follow SMAD dependent as well as SMAD-independent signaling pathways depending upon cellular …

Gene Delivery In The Equine Cornea: A Novel Therapeutic Strategy, Dylan G. Buss, Ajay Sharma, Elizabeth A. Giuliano, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To determine if hybrid adeno-associated virus serotype 2/5 (AAV5) vector can effectively deliver foreign genes into the equine cornea without causing adverse side effects. The aims of this study were to: (i) evaluate efficacy of AAV5 to deliver therapeutic genes into equine corneal fibroblasts (ECFs) using enhanced green fluorescent protein (EGFP) marker gene and (ii) establish the safety of AAV5 vector for equine corneal gene therapy.

Animal Material—Primary ECF cultures were harvested from healthy donor equine corneas. Cultures were maintained at 370C in humidified atmosphere with 5% CO2.

Procedure—AAV5 vector expressing EGFP under control of hybrid cytomegalovirus (CMV) + chicken …

Rumen Microbial Population Dynamics During Adaptation To A High-Grain Diet, Samodha C. Fernando, H. T. Purvis Ii, F. Z. Najar, L. O. Sukharnikov, C. R. Krehbiel, T. G. Nagaraja, B. A. Roe, Udaya Desilva

Samodha C. Fernando Publications

High-grain adaptation programs are widely used with feedlot cattle to balance enhanced growth performance against the risk of acidosis. This adaptation to a high-grain diet from a high-forage diet is known to change the rumen microbial population structure and help establish a stable microbial population within the rumen. Therefore, to evaluate bacterial population dynamics during adaptation to a high-grain diet, 4 ruminally cannulated beef steers were adapted to a high-grain diet using a step-up diet regimen containing grain and hay at ratios of 20:80, 40:60, 60:40, and 80:20. The rumen bacterial populations were evaluated at each stage of the step-up …

Efficacy And Safety Of Mitomycin C As An Agent To Treat Corneal Scarring In Horses Using An In Vitro Model, Dylan G. Buss, Ajay Sharma, Elizabeth A. Giuliano, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—Mitomycin C (MMC) is used clinically to treat corneal scarring in human patients. We investigated the safety and efficacy of MMC to treat corneal scarring in horses by examining its effects at the early and late stages of disease using an in-vitro model.

Procedure—An in-vitro model of equine corneal fibroblast (ECF) developed was used. The equine corneal fibroblast or myofibroblast cultures were produced by growing primary ECF in the presence or absence of transforming growth factor beta-1 (TGFβ1) under serum-free conditions. The MMC dose for the equine cornea was defined with dose-dependent trypan blue exclusion and MTT [(3-4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays …

Isolation And Cultivation Of Equine Corneal Keratocytes, Fibroblasts And Myofibroblasts, Dylan G. Buss, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To establish an in vitro model for the investigation of equine corneal wound healing. To accomplish this goal, a protocol to isolate and culture equine corneal keratocytes, fibroblasts and myofibroblasts was developed.

Animal material—Equine corneal buttons were aseptically harvested from healthy research horses undergoing humane euthanasia for reasons unrelated to this study. Slit-lamp biomicroscopy was performed prior to euthanasia by a board-certified veterinary ophthalmologist to ensure that all samples were harvested from horses free of anterior segment disease.

Procedure—Equine corneal stroma was isolated using mechanical techniques and stromal subsections were then cultured. Customized media at different culture conditions was used …

Epigenetics: Blurring The Line Between Nature And Nurture, Elizabeth H. Rose

CMC Senior Theses

This long-standing nature versus nurture debate is cited in behavioral and physical expressions of disease dysfunctions, resiliencies, and recovery. Their purposes are noted both in scientific pursuits as well as literature. This discourse has been particularly intense in the fields of psychology, psychiatry, and biology where there is a long history of scientists’ attempts to disprove or discredit others’ intellectual and professional measures. Interestingly, recent advances in the neurosciences and genetic technologies have brought these fields closer together with a new focus – the interactional relationship between nature and nurture – epigenetics.

Vector Delivery Technique Affects Gene Transfer In The Cornea In Vivo, Rajiv R. Mohan, Ajay Sharma, Tyler C. Cebulko, Ashish Tandon

Pharmacy Faculty Articles and Research

Purpose: This study tested whether controlled drying of the cornea increases vector absorption in mouse and rabbit corneas in vivo and human cornea ex vivo, and studied the effects of corneal drying on gene transfer, structure and inflammatory reaction in the mouse cornea in vivo.

Methods: Female C57 black mice and New Zealand White rabbits were used for in vivo studies. Donor human corneas were used for ex vivo experiments. A hair dryer was used for drying the corneas after removing corneal epithelium by gentle scraping. The corneas received no, once, twice, thrice, or five times warm air for …

In Remembrance Of Denis Alan Trevor New, Robert L. Brent Dr.

The Selected Works of Robert Brent

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Ionizing Radiation, Robert L. Brent Dr.

The Selected Works of Robert Brent

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Safety Assessment For Tipranavir : A Protease Inhibitor Treatment For Hiv Infected Adults, Jaromir Mikl

Legacy Theses & Dissertations (2009 - 2024)

Dose-limiting adverse events associated with anti-retroviral therapy (ART) generally include gastro-intestinal (GI) issues such as vomiting and nausea. However, serious adverse events (SAEs) do occur, the most frequent of which include liver toxicities and renal impairment as well as recent evidence of cardio-vascular disease (CVD) risk.

Effects Of Adenosine A1 Receptor Agonist Ccpa And Antagonist Dpcpx On Ischemia/Reperfusion-Induced Arrhythmias In Rats*, Ömer Bozdoğan, Ersöz Gonca, Nuran Ekerbi̇çer

Turkish Journal of Medical Sciences

Adenosine has been commonly used to treat supraventricular tachycardia in clinics. However, there are only a few studies on the effects of adenosine on ischemia or reperfusion induced arrhythmia and they conflict with the results of the present study. During this study, we aimed to clarify the effect of adenosine on ischemia-reperfusion induced arrhythmia. Materials and methods: Left coronary artery was ligated for 6 min and it was released for 15 min to produce reperfusion. A1 adenosine agonist CCPA (2 Chloro - N6 - Cyclopentyl-adenosine), and A1 selective antagonist DPCPX (8 - Cyclopentyl - 1,3 -dipropylxanthine) in 5 µg/kg dose …

Morphine Modulates Microvascular Leakage Dose-Dependently In The Airway Of Ovalbumin-Sensitized Rats, Seyfullah Oktay Arslan

Turkish Journal of Medical Sciences

To investigate the effect of morphine on ovalbumin-evoked airway microvascular leakage in sensitized rats. Materials and methods: Rats were sensitized on days 0, 14, and 21 with ovalbumin. Intravenous ovalbumin (2 mg/kg) or capsaicin (50 µg/kg) increased the extravasation of Evans blue dye in trachea, bronchi, and intra-pulmonary tissues of sensitized rats. Results: Morphine (1-10 mg/kg) inhibited ovalbumin-evoked increase in microvascular plasma leakage in a dose-dependent manner; however, it had no significant effect at the doses of 0.1 or 30 mg/kg. In addition, morphine, at the doses of 1-30 mg/kg, abolished microvascular leakage increased by capsaicin. The inhibition caused by …

The Effect Of L-Tryptophan On The Heart In Rabbits Via Chronic Hypoxia, Fi̇gen Nari̇n, Nazmi̇ Nari̇n, Fatmagül Başarslan, Ali̇ Baykan, Sadetti̇n Sezer, Hülya Akgün, Aynur Akin, Mustafa Akçakuş, Hakan Ceyran

Turkish Journal of Medical Sciences

To evaluate the protective effect of tryptophan on an experimentally produced hypoxic myocardial injury via biochemical and pathological parameters. Materials and methods: A total of 26 rabbits were divided into 3 groups. Group 1 (n = 9) was only exposed to hypoxia. Group 2 (n = 10) was exposed to hypoxia and received L-tryptophan (200 mg/kg per day, orally for 5 days). Group 3 (n = 7) was the control group. Before the hypoxic injury and after the delivery of the medication, serum samples were taken for troponin-I, creatine kinase myocardial isoenzymes (CK-MB), lactate dehydrogenase (LDH), glutathione peroxidase (GSH-Px), superoxide …

Implications Of Socio-Economic Status On The Dietary Fatty Acid Intakes In Turkish Women, Zehra Büyüktuncer, Berat Nursal Tosun, Ayli̇n Ayaz, Makbule Gezmen Karadağ, Alev Kocabaş Keser, Meral Aksoy, Mi̇ne Yurttagül, Sevi̇nç Yücecan, Hali̇t Tanju Besler

Turkish Journal of Medical Sciences

To investigate the effects of socio-economic status (SES) on the dietary intakes of fatty acids in a group of Turkish women. Materials and methods: Five hundred and sixty three women were randomly enrolled into the study. They were classified into 3 SES groups: group 1 high (n = 186), group 2 medium (n = 186), and group 3 low (n = 191). The groups were determined by the SES distribution of Ankara as determined by the 2000 census. Each participant was investigated with a questionnaire consisting of demographic characteristics, physical activity level (24-h record), and dietary intake (24-h recall) by …

Exposure To Continuous Darkness Leads To Atypical Symptoms Of Seasonal Affective Disorder In Rats, Çağla Ayhan İşman, Nesli̇han Toyran, Ni̇met Ünay Gündoğan

Turkish Journal of Medical Sciences

Prolonged duration of nocturnal melatonin increase has been implicated in the pathogenesis of the seasonal affective disorder (SAD) winter depression. This study was designed to investigate behavioral and physiological consequences of constant dark exposure in male rats for its potential application to human affective disorders. Materials and methods: A total of 12 male Wistar-Albino rats (230-290 g) were assigned to control (LD 12:12 h; n = 6) and constant darkness (DD, 10 days; n = 6) groups. Experimental protocol included comparison of behavioral (FST, open field test, and sucrose preference) and physiological (body weight, food intake, and blood glucose level) …

Anti-Diabetic Activity Of Tinospora Cordifolia (Willd.) In Streptozotocin Diabetic Rats; Does It Act Like Sulfonylureas?, Nagaraja Puranik, Kararashah Fakruddin Kammar, Sheela Devi

Turkish Journal of Medical Sciences

As an alternative medicine to treat diabetes mellitus, many herbal drugs are being studied throughout the world. In the present study, an attempt was made to investigate the anti-diabetic activity of Tinospora cordifolia (Willd.) (TC) stem extracts (both aqueous and alcoholic) in different dosages (200 and 400 mg/kg b.w.) in streptozotocin diabetic albino rats. The probable mechanism by which TC may act as an anti-hyperglycemic drug was also investigated. Materials and methods: The drug was administered orally for 10 days and 30 days in different groups of animal, with each group containing 6 animals. The efficacy of this drug was …

Isolation Of Cryptococcus Neoformans From Pigeon Droppings In Ahwaz, Iran, Majid Zarrin, Masoomeh Jorfi, Nasrin Amirrajab, Morad Rostami

Turkish Journal of Medical Sciences

Cryptococcus neoformans is an opportunistic human pathogen that causes cryptococcosis, a life-threatening infection that is usually manifested as menigeoencephalitis mainly in immunocompromised patients. The objective of this study was to evaluate the presence of Cryptococcus neoformans in Ahwaz, Iran. Materials and methods: Sixty-five samples of pigeon droppings were collected from 10 different regions in Ahwaz. Each sample was suspended 1:10 in saline solution and then cultured in Sabouraud's dextrose agar medium including chloramphenicol. Identification of C. neoformans was performed on the basis of melanin synthesis on bird seed agar, presence of a capsule on India ink preparation, urease production on …

Effect Of Viscum Album On Acute Hepatic Damage Caused By Carbon Tetrachloride In Rats, Omar Mohamed Abdel-Salam, Amany Ameen Sleem, Nermeen M. Shaffie

Turkish Journal of Medical Sciences

To investigate the effect of Viscum album, a plant used for the treatment of hepatocellular carcinoma that has immune-modulating properties, on acute hepatic injury in rats. Materials and methods: Hepatotoxicity was induced by CCl_4 orally (0.28 mL/kg). Rats received either Viscum album at 1 of 2 dose levels (0.1 or 0.2 mL/kg) once weekly subcutaneously alone or with silymarin (25 mg/kg, orally), or silymarin (25 mg/kg) once daily orally for 1 month, starting at the time of administration of CCl_4. Liver damage was assessed by determining liver serum enzyme activities and by hepatic histopathology. Results: Viscum album administration decreased the …

Correlation Between Increased Urinary Serotonin Levels And Coronary Artery Disease In Cigarette Smoking Patients, Necat Yilmaz, Yakup Mermerdaş, Esi̇n Eren, Ayşenur Yeği̇n, Emi̇ne Namiduru

Turkish Journal of Medical Sciences

Platelets secrete serotonin (5-hydroxytryptamine), which has several effects on the vascular wall and promotes thrombogenesis, mitogenesis, and the proliferation of smooth muscle cells. We therefore measured excreted levels of 5-hydroxytryptamine (5-HT) concentrations in urine as a means of assessing vascular lesions in patients with coronary artery disease ( CAD). Materials and methods: Subjects who underwent coronary angiography were classified into 2 groups according to their diagnosis. The CAD group consisted of 33 patients with coronary artery stenosis, whereas the healthy control group consisted of 30 subjects. An isocratic high-performance liquid chromatographic (HPLC) system with an electrochemical detector was used for …

Caustic Esophageal Injury Decreases The Number Of Interstitial Cells Of Cajal In The Rat Esophagus, Tutku Soyer, Şebnem Ayva, Sali̇h Somuncu, Pinar Atasoy, Turan Kanmaz, Murat Çakmak

Turkish Journal of Medical Sciences

To evaluate the effect of caustic esophageal injury (CEI) on the number of interstitial cells of Cajal (ICC). Materials and methods: Wistar rats were divided into 3 groups: control, acid, and alkali induced CEI. CEI was performed by the injection of 1 mL of 10% hypochloric acid in the acid group, and 1 mL of 10% sodium hydroxide was injected into the alkali group. Distal esophageal segments were removed 24 h after injection. CEI was graded and the number of ICC were investigated (CD-117 staining). The number of ICC was compared in groups and correlated with CEI grades. Results: Typical …

The Effects Of 3-Benzoyl-1-Methyl-4-Phenyl-4 Piperidinolhydrochloride (C1), Indomethacin, Nimesulide And Rofecoxib On Cyclooxygenase Activities In Carrageenan-Induced Paw Edema Model, Yaşar Nuri̇ Şahi̇n, Berna Demi̇rcan, Hali̇s Süleyman, Hülya Aksoy, Hali̇se İnci̇ Gül

Turkish Journal of Medical Sciences

The aim of this study was to investigate the effects of 3-benzoyl-1-methyl-4-phenyl-4-piperidinol-hydrochloride (C1), which is a structural and also non-classical isomer of bis Mannich base, bis (3-aryl-3-oxo-propyl) methylamine hydrochlorides (B1), on cyclooxygenase (COX) activities in 48 rats with inflammation by using carrageenan-induced paw edema, and to compare its effect with other non-steroidal anti-inflammatory drugs (NSAIDs). Materials and methods: Experimental animals were supplied by the Center of Experimental Research and Practice in Ataturk University. The animals were housed and fed in the laboratory (normal room temparature, food and water) under standard conditions. Rats were randomly assigned to eight groups (n = …

Adrenergic And Cholinergic Responses Of The Tracheal Smooth Muscle Is Enhanced During Estrus Phase In Rat, M. Kasim Çayci, Yasemi̇n Aydin, Kubi̇lay Uzuner

Turkish Journal of Medical Sciences

The aim of this study was to evaluate influences of hormonal changes during rat estrous cycle on airway responsiveness to bronchoconstrictor and bronchodilator agents. Materials and methods: The study was performed in 5 groups of rat tracheal smooth muscle obtained during proestrus, estrus, metestrus, and diestrus phases of the estrous cycle, as well as the ovariectomized group (n:8/group). Cumulative acetylcholine contractions ranging from 10^{-8} to 10^{-3} M bath doses and the cumulative isoproterenol relaxations ranging from 10^{-8} to 10^{-3} M precontracted with 10^{-5} M of acetylcholine were recorded on isolated tracheas placed in a computerized tissue bath system. Results: Acetylcholine-induced …

Crimean-Congo Hemorrhagic Fever Cases In The Eastern Black Sea Region Of Turkey: Demographic, Geographic, Climatic, And Clinical Characteristics, Nurgün Günaydin, Kemaletti̇n Aydin, Gürdal Yilmaz, Hati̇ce Rahmet Çaylan, İftahar Köksal

Turkish Journal of Medical Sciences

Crimean-Congo hemorrhagic fever (CCHF) is a disease that is known since 1944 affecting many people across the world and causing a high mortality rate. The aim of this study was to determine the epidemiological and clinical characteristics of the CCHF cases in our region. Materials and methods: The present study was conducted in patients with CCHF who were hospitalized between 2004 and 2006 at Karadeniz Technical University. Demographic, geographic, climatic, and clinical characteristics of all patients were investigated. Results: A total of 102 patients with confirmed CCHF were investigated in this study. All of the patients were from rural areas. …

The Effect Of Caffeic Acid Phenethyl Ester On Bacterial Translocation And Intestinal Damage After Intestinal Obstruction, Cengi̇z Ara, Abuzer Di̇ri̇can, Seli̇m Erdoğan, Burhan Ateş, Di̇nçer Özgör, Fai̇k Tatli, M. Sai̇t Tekerekoğlu, Vedat Kirimlioğlu

Turkish Journal of Medical Sciences

Intestinal obstruction (IO) induces bacterial translocation due to failure of the intestinal barrier function. Following bacterial overgrowth, its degradation products play a decisive role in the development of systemic septic complications. The aim of this study was to evaluate the effects of caffeic acid phenethyl ester (CAPE) on bacterial translocation and intestinal damage in an IO model in rats. Materials and methods: Complete IO was created in the distal ileum of rats by a single 4-0 silk suture. A total of 21 Wistar albino rats were randomized into 3 groups: Group 1, Sham (n = 7); Group 2, IO (n …

Chlorella Viruses Encode Most, If Not All, Of The Machinery To Glycosylate Their Glycoproteins Independent Of The Endoplasmic Reticulum And Golgi, James L. Van Etten, James Gurnon, Giane M. Yanai-Balser, David Dunigan, Michael V. Graves

Nebraska Center for Virology: Faculty Publications

In contrast to all other viruses that use the host machinery located in the endoplasmic reticulum and Golgi to glycosylate their glycoproteins, the large dsDNA-containing chlorella viruses encode most, if not all, of the components to glycosylate their major capsid proteins. Furthermore, all experimental results indicate that glycosylation occurs independent of the endoplasmic reticulum and Golgi. (Review article)

Loss Of The Sin3 Transcriptional Corepressor Results In Aberrant Mitochondrial Function, Valerie L. Barnes, Bethany S. Strunk, Icksoo Lee, Maik Hüttemann, Lori A. Pile

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

SIN3 is a transcriptional repressor protein known to regulate many genes, including a number of those that encode mitochondrial components.

Results

By monitoring RNA levels, we find that loss of SIN3 inDrosophilacultured cells results in up-regulation of not only nuclear encoded mitochondrial genes, but also those encoded by the mitochondrial genome. The up-regulation of gene expression is accompanied by a perturbation in ATP levels in SIN3-deficient cells, suggesting that the changes in mitochondrial gene expression result in altered mitochondrial activity. In support of the hypothesis that SIN3 is necessary for normal mitochondrial function, yeastsin3null mutants exhibit very poor …

Definitions Of The Phenotypic Manifestations Of Sickle Cell Disease., Samir K Ballas, Susan Lieff, Lennette J Benjamin, Carlton D Dampier, Matthew M Heeney, Carolyn Hoppe, Cage S Johnson, Zora R Rogers, Kim Smith-Whitley, Winfred C Wang, Marilyn J Telen

Department of Medicine Faculty Papers

Sickle cell disease (SCD) is a pleiotropic genetic disorder of hemoglobin that has profound multiorgan effects. The low prevalence of SCD ( approximately 100,000/US) has limited progress in clinical, basic, and translational research. Lack of a large, readily accessible population for clinical studies has contributed to the absence of standard definitions and diagnostic criteria for the numerous complications of SCD and inadequate understanding of SCD pathophysiology. In 2005, the Comprehensive Sickle Cell Centers initiated a project to establish consensus definitions of the most frequently occurring complications. A group of clinicians and scientists with extensive expertise in research and treatment of …

Cellular And Molecular Dissection Of Pluripotent Adult Somatic Stem Cells In Planarians, Norito Shibata, Labib Rouhana, Kiyokazu Agata

Biological Sciences Faculty Publications

Freshwater planarians, Plathelminthes, have been an intriguing model animal of regeneration studies for more than 100 years. Their robust regenerative ability is one of asexual reproductive capacity, in which complete animals develop from tiny body fragments within a week. Pluripotent adult somatic stem cells, called neoblasts, assure this regenerative ability. Neoblasts give rise to not only all types of somatic cells, but also germline cells. During the last decade, several experimental techniques for the analysis of planarian neoblasts at the molecular level, such as in situ hybridization, RNAi and fluorescence activated cell sorting, have been established. Moreover, information about genes …