Digital Commons Network^™

The Eco-Evolutionary Consequences Of Spatial Patterning Of Macroalgal Life Cycle Stages And Reproductive Mode Variation, Alexis Oetterer

All ETDs from UAB

Haploid-diploid life cycles offer valuable insight into the evolution of sex and ploidy cycling, but empirical research has largely focused on diploid-dominant taxa, such as angiosperms. Expanding empirical studies to include a broader range of eukaryotic diversity is critical to ultimately understand responses to climate change. This dissertation addresses gaps in our understanding of macroalgal reproductive modes by examining macroalgae Gracilaria vermiculophylla and Ulva spp. along the Delmarva Peninsula. To understand how hard and soft bottom habitats have structured genetic diversity and reproductive modes in Gracilaria vermiculophylla and how these habitats have shaped colonization of this area, we used nine …

Interactions Between Staphylococcus Aureus And Corynebacterium Species In The Context Of Chronic Rhinosinusitis, Joshua Tucker Huffines

All ETDs from UAB

Chronic rhinosinusitis (CRS) is a complex inflammatory disease affecting the upper respiratory tract (URT) for a large portion of the populace, leading to significant economic burdens. Staphylococcus aureus is considered as a significant contributor to CRS inflammation through the production of toxins regulated through quorum sensing (QS). Microbiome studies have characterized the microbial communities present in the healthy URT and in CRS. These studies show that the genus Corynebacterium is associated with the healthy URT, decreased in CRS, and has a negative correlation with S. aureus regardless of health status. A few studies have partially characterized a mechanism to explain …

Virulence Factors And Their Binding Partners: A Biochemical Study, Morgan E. Blake

All ETDs from UAB

Virulence factors are an important mechanism by which viruses combat the host immune system, therefore a thorough understanding of the workings of these proteins will be of significant value in prevention of disease associated with viral infections. In this dissertation we investigate the main virulence factors of two viruses, the Influenza A Virus (IAV) and SARS-CoV-2, and their interactions with host proteins, providing critical information for structure-based drug design. In chapter one we studied the nonstructural protein 1 (NS1) of IAV and its interaction with the 30-kda subunit of the cleavage and polyadenylation specificity factor (CPSF30) to inhibit host cell …

Standardizing Sea Urchin Husbandry: Tank Effects, Gamete Production, And Co-Culture, Raven Amber Edwards

All ETDs from UAB

This dissertation seeks to further knowledge of sea urchin husbandry in three major areas that have so far not been investigated substantially: optimal conditions for housing sea urchins in a lab-based recirculating aquaculture systems (RAS), impacts of repetitive spawning regimens on reproductive output, and co-culture utilizing juvenile oysters. All studies utilize a commercial recirculating aquaculture system that is relatively novel for culturing sea urchins. The first chapter investigated the influence of housing conditions on sea urchin growth outcomes. Under the conditions of this study, the size of the tank showed limited influence on growth; however, stocking density was found to …

Structural And Functional Investigation Of The Influenza Nuclear Export Protein, Andrew James Staup

All ETDs from UAB

The influenza A nuclear export protein (NEP) is a multi-functional protein that mediates the export of progeny viral ribonucleoprotein (vRNP) complexes from the host nucleus, regulates the activity of the RNA-dependent RNA polymerase (RdRp), and is implicated in zoonosis. It is therefore critical to understand the mechanisms of NEP’s functions at the molecular level to inform its roles in the viral lifecycle and influenza zoonosis. In this dissertation, we report the structure of NEP in complex with the host nuclear export holo-complex as well as investigate the biophysical effects of the adaptive M16I mutation on NEP. We solved the cryo-electron …

Air Pollution And Environmental Injustice Are Related To Respiratory Complications In Individuals With Sickle Cell Disease, Kelsey M. Maclin

All ETDs from UAB

Sickle cell disease (SCD) is a genetically inherited blood disorder affecting people of African descent. SCD is characterized by painful episodes and an acute lung injury known as acute chest syndrome (ACS). Patients with SCD have a life expectancy <19 years compared to Blacks without SCD. There is minimal research investigating air pollution and health complications in SCD. The aims of this research were to investigate whether pulmonary function is affected by exposure to ambient air pollution, whether higher air pollution concentrations are associated with hospitalizations for ACS, and whether environmental injustice impacts the respiratory health of children and young adults with SCD. Air pollution data on the criteria air pollutants (CAPs) was obtained from the US Environmental Protection Agency (EPA). Patient health data were obtained from Children’s Hospital of Alabama electronic medical records. Logistic regression, distributed lag non-linear regression, Granger Causality, and Getis-Ord Gi* hot spot analysis were used to analyze our data. The EPA’s EJScreen tool was used as a measure of exposure to environmental injustice. ArcGIS was used to map the EJScreen data. Exposure to short-term air pollution is significantly associated with a reduction in pulmonary function in children and young adults with SCD. Children and young adults exposed to a one-unit increase in the monthly average concentrations of sulfur dioxide (SO2) experienced an increase in the odds of having abnormal pulmonary function. We observed the trends of each criteria air pollutant in our study to be closely aligned with the trends in the hospitalizations for ACS. Most of our study participants with a history of ACS lived in areas burdened by greater environmental injustices. Specifically, the hot spots for ACS overlapped with the hot spots for PM2.5. Unequal exposure to environmental injustices leads to increases in hospitalizations for respiratory complications in individuals with SCD. Reductions in air pollution could be greatly beneficial to the pulmonary function and overall respiratory health of individuals with SCD. Further research is needed to examine associations between air pollution and hospitalizations for ACS in individuals with SCD.

Accelerations And Emg Differences Between Isocaloric High-Incline Walking And Level-Grade Jogging, Taj Krieger, Motoki Sato, Lex Gidley, Justus Ortega, Eli Lankford

International Journal of Exercise Science

International Journal of Exercise Science 18(7): 995-1009, 2025. High-incline walking is a popular mode of exercise and often serves as an alternative to level-grade running for improving cardiovascular fitness. This study examined the muscle activation and biomechanical differences between high-incline walking (HIW) at a 20% grade and level-grade jogging (LGJ) at matched exercise intensities. Nineteen physically inactive adults (18-31 years) participated. Participants completed two isocaloric exercise trials (HIW and LGJ), each lasting ten minutes. EMG data were obtained from eight muscles: biceps femoris (BF), gluteus maximus (GM), lateral gastrocnemius (LG), tibialis anterior (TA), vastus lateralis (VL), anterior deltoid (AD), erector …

Editorial: A Step-By-Step Statistical Decision Framework For A Gender-Inclusive Approach In Sport And Exercise Science Research, James W. Navalta, Dustin W. Davis, Jafra D. Thomas, Whitley J. Stone

International Journal of Exercise Science

International Journal of Exercise Science 18(1): 1010-1029, 2025. The conflation of sex and gender in sport and exercise science research has led to gaps in representation and understanding of gender-inclusive outcomes. This invited editorial presents a framework to guide researchers in conducting statistical analyses that account for multiple gender identities beyond the traditional binary classification. The invited editorial guides deliberation on whether to test for sex or gender differences in sport or exercise science research. It prompts investigators to answer the question, “Is there a valid, literature-supported rationale for investigating sex or gender differences?” If “yes”, we propose approaches that …

The Cost Of Malnutrition In Older Adults In The Mountain West, 2020, Taylor Volk, Anirudh Polagani, Olivia K. Cheche, Kristian Thymianos, Caitlin J. Saladino, William E. Brown Jr.

Health

This fact sheet presents 2020 data on the annual costs of disease-associated malnutrition for adults aged 65 years and older across the five Mountain West states of Arizona, Colorado, Nevada, New Mexico, and Utah. The data are sourced from “Advancing Policies for Quality Malnutrition Care in Older Adults: A Toolkit for State Legislators,” a report published by Defeat Malnutrition Today and Women in Government.

Optimizing Plant Nutrient Uptake: Cost Function Sensitivity Analysis Of The Fun Model, Gabriel Alpay, Joshua B. Fisher, Renato K. Braghiere, Edward R. Brzostek, Gabriella R. Dauber, Kara Allen, Rosemary A. Fisher

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

This study explores the cost functions of the Fixation & Uptake of Nutrients (FUN) model, which aims to optimize the amount of carbon used when plants acquire their necessary nutrients from the soil to maximize nitrogen (N) and phosphorus (P) uptake while minimizing the carbon cost. Specifically, we aimed to quantify the sensitivity of FUN outputs to the parameters belonging to the cost of nitrogen and phosphorus uptake functions by arbuscular mycorrhizal and ectomycorrhizal fungi. We present three dimensions of sensitivity analysis: (1) adjusting all parameters at the same time; (2) keeping one parameter constant while varying the other; and, …

Details Matter: Getting People Involved, Cheryl A. Burkhart-Kriesel

Cornhusker Economics

Sometimes getting people into the habit of community participation can be a bit challenging, but two aspects can help make this a little easier. The first is to make sure that there are no organizational barriers in participating, and the second is to realize that community participation happens on several levels and that each level has certain expectations. When these expectations are clearly stated, people are more easily motivated to get involved and stay involved.

Setting up engagement from the beginning requires thought and attention to detail. It can be insignificant things, like choosing a date or picking a location, …

Innate And Adaptive Immune Features Associated With Immune-Related Adverse Events, Shaheen Khan, Venkat S Malladi, Mitchell S Von Itzstein, Hong Mu-Mosley, Farjana J Fattah, Yang Liu, Mary E Gwin, Jason Y Park, Suzanne M Cole, Sheena Bhalla, Jay Lohrey, David Hsiehchen, Angela Moses, Tao Wang, Yaming Xue, Angela B Mobley, J David Farrar, Marjaan Imam, Michelle Wu, Quan-Zhen Li, Edward K Wakeland, Yang Xie, Jeffrey A Sorelle, David E Gerber

Faculty, Staff and Student Publications

Background: While highly efficacious for numerous cancers, immune checkpoint inhibitors (ICIs) can cause unpredictable and potentially severe immune-related adverse events (irAEs), underscoring the need to understand irAE biology.

Methods: We used a multidimensional approach incorporating single-cell RNA sequencing, mass cytometry, multiplex cytokine assay, and antinuclear antibody (ANA) profiling to characterize the peripheral immune landscape of patients receiving ICI therapy according to irAE development.

Results: Analysis of 162 patients revealed that individuals who developed clinically significant irAEs exhibited a baseline proinflammatory, autoimmune-like state characterized by a significantly higher abundance of CD57+ T and natural killer (NK) T cells, plasmablasts, proliferating and …

Bypassing Bamd Essentiality By Mutations In A Non-Essential Substrate, Santosh Kumar, Anna Konovalova

Faculty, Staff and Student Publications

The β-barrel assembly machinery (Bam) is essential for assembling all transmembrane β-barrel outer membrane proteins in gram-negative bacteria. The Bam complex consists of the central β-barrel protein BamA and accessory Bam lipoproteins, including the widely conserved and essential BamD. BamD is assumed to be an essential regulator of OMP assembly, as its absence causes a global defect in OMP biogenesis. Here, we challenge this view by demonstrating that BamD essentiality is both conditional and substrate specific. In Escherichia coli, its function can be bypassed by preventing BamA jamming by a single, non-essential substrate RcsF. Our findings suggest that BamD …

Infectious Prions In Brains And Muscles Of Domestic Pigs Experimentally Challenged With The Bse, Scrapie, And Cwd Agents, Francisca Bravo-Risi, Fraser Brydon, Angela Chong, Kane Spicker, Justin J Greenlee, Glenn Telling, Claudio Soto, Sandra Pritzkow, Marcelo A Barria, Rodrigo Morales

Faculty, Staff and Student Publications

Experimental studies suggest that animal species not previously described as naturally infected by prions are susceptible to prion diseases affecting sheep, cattle, and deer. These interspecies transmissions may generate prions with unknown host ranges. Pigs are susceptible to prions from different origins, including deer chronic wasting disease (CWD), sheep scrapie, and bovine spongiform encephalopathy (BSE). Here, we studied prions in brains and muscles from pigs previously infected with these different prion sources. Specifically, we measured the total prion protein (PrP) and PK-resistant PrP by western blot. Seeding activity in these tissues was evaluated using the protein misfolding cyclic amplification (PMCA) …

Inhibition Of The Ufd-1-Npl-4 Complex Triggers An Aberrant Immune Response In Caenorhabditis Elegans, Rajneesh Rao, Alejandro Aballay, Jogender Singh

Faculty, Staff and Student Publications

The UFD-1 (ubiquitin fusion degradation 1)-NPL-4 (nuclear protein localization homolog 4) heterodimer is involved in extracting ubiquitinated proteins from several plasma membrane locations, including the endoplasmic reticulum. This heterodimer complex helps in the degradation of ubiquitinated proteins via the proteasome with the help of the AAA+ATPase CDC-48. While the ubiquitin-proteasome system is known to have important roles in maintaining innate immune responses, the role of the UFD-1-NPL-4 complex in regulating immunity remains elusive. In this study, we investigate the role of the UFD-1-NPL-4 complex in maintaining

Prairie Soundscape, Rebecca J. Romsdahl

The Cicadian

This series of soundscapes represents dawn and dusk. Morning Cacophony was recorded at 6:00 AM, May 2023 on the Tympanuchus Reserve near Crookston, MN — a rare remnant prairie never plowed. Voices of the prairie: sedge wren, bobolink, greater prairie chicken, sandhill crane, red-winged blackbird, clay-colored sparrow. Night Chorus was captured at 2:00 AM, June 2024 on restored grassland at Bluestem Preserve near Moorhead, MN — once farmland, now thriving prairie. Nocturnal symphony: whip-poor-will (song + warning call), coyotes, treefrogs, grasshopper sparrow, soft wind, gentle rain.

Gardens As Pathways To Environmental Education For All Ages, Lacey Anderson

The Cicadian

Gardens provide hands-on opportunities for environmental education that extend beyond food production, encompassing ecological literacy, sustainability practices, experiential learning, and community engagement. This article uses a creative nonfiction approach to examine the author’s personal journey from reluctant gardener to advocate for learning gardens. Through narrative reflection of her own family’s gardening experiences during the COVID-19 pandemic, the author illustrates how gardening fosters intergenerational learning, socio-emotional development, and curiosity-driven exploration of ecological systems. The article highlights how gardens function as accessible entry points for lifelong learners of all ages, demonstrating that environmental literacy can emerge outside of traditional scientific expertise. Ultimately, …

Where The Earth Meets The Sky, Ashlyn Ed Herron

The Cicadian

A reflection of finding one's place in an ecosystem that raised them, and a long overdue love-letter to the prairies

The Effect Of A Kn95 Mask On High-Intensity Interval Training Performance, Physiological Response, And Perception, David Kenny, Payton Stanziani, Holly Chambers, Madison Vinovrski, Isabel Allocco, Jack Fisher, Morgan Raley, Kyle S. Beyer

International Journal of Exercise Science

International Journal of Exercise Science 18(3): 984-994, 2025. The purpose of this study was to assess the effect of a KN95 mask on the performance, physiological response, and perception of high-intensity interval training (HIIT). Twenty college-aged participants (Male: 10, Female: 10) completed a two-visit, crossover, counterbalanced study. The HIIT workout included four 1-minute “all-out” intervals at 3.5% of body mass with 4-minute rest intervals, performed with or without a KN95 mask. Following HIIT, participants remained on the cycle ergometer for a 15-minute recovery period. For each interval, peak power and average power were recorded. Throughout the HIIT workout and recovery, …

Evaluating The Impact Of Zoa Energy Drink On Muscular Strength, Psychomotor Vigilance, And Mood In Active Adults: A Randomized Controlled Trial, Jose J. Rojas, Jason M. Curtis, Flavia F. Pereira, Cassandra Evans, Kaelan Jones, Alyana Andal, Juan Carlos Santana, Hena Thakkar, Jose Antonio

Journal for Sports Neuroscience

This study evaluates the effects of ZOA Energy Drink, a commercially available energy beverage, on muscular strength, sustained attention, and mood in exercise-trained individuals. Using a randomized, blinded, two-arm design, 24 participants (15 males, 9 females; age = 31.8 ± 8.4 years) were assigned to consume either ZOA Energy Drink or a caffeine-matched placebo. The primary outcomes included psychomotor vigilance (PVT), mood state (POMS), handgrip strength, and push-up performance. Baseline body composition was also assessed using bioelectrical impedance.

Results revealed no statistically significant differences between the ZOA and the caffeine matched control groups in psychomotor vigilance (p = 0.155), mood …

The Genus Androctonus Ehrenberg, 1828 (Scorpiones: Buthidae) In Oman, Graeme Lowe

Euscorpius

The taxonomic position of Androctonus populations in Oman was investigated by analyzing morphological variation in a comprehensive sample representing 150 records gathered from 95 localities spread throughout the country. Two new allopatric species are described, separated by multivariate morphometric analysis and meristic characters: (1) A. omanensis sp. n., distributed across most of Oman, is characterized by wider, deeper metasomal segments IV–V, shorter legs, more robust pedipalps, more granules on lateral inframedian carinae of metasoma II–III, and sparser setation on basitarsal spurs; and (2) A. ammophilus sp. n., distributed in the dunes of the Rub’ al-Khali (Empty Quarter) of …

Exploring The Relative Effects Of Natural Gas And Biogas Cooking On Indoor Air Quality In Residential Kitchens, Wande Benka-Coker, Kailey Sipe, Dinela Dedic, Alexander Jones, Bramley Hawkins, Emily Lyons, Matt Steiman, Megan L. Benka-Coker

Health Sciences Faculty Publications

Indoor air pollution from gas stove combustion remains a public health concern, given links to adverse cardiorespiratory health effects, yet few studies have characterized or compared the air quality impacts of different gas-based cooking fuels. We investigated kitchen-level concentrations of nitrogen dioxide (NO2) and fine particulate matter (PM2.5) concentrations in four homes in Central Pennsylvania that used natural gas and/or biogas fueled stoves. We conducted time-resolved kitchen monitoring and assessed pollutant concentrations during cooking and non-cooking periods. We applied linear mixed-effect regression models with kitchen-level random effects and time-varying covariates to estimate the influence of fuel type on indoor air …

Beneficial Effects Of Performlytetm, A Prodosomed Phytonutrient-Enriched Electrolyte Formulation, In Enhancing Neuro-Muscular Synergy, Immune Competence, Cellular Aerobic Glycolysis And Sports Performance, Bernard William Downs, Samudra P. Banik, Bruce S. Morrison, Tandra Chakraborty, Manashi Bagchi, Sanjoy Chakraborty, Steve Kushner, Debasis Bagchi

Publications and Research

An optimised sports nutrition formulation achieving the correct blend of electrolytes with antioxidants and vitamins is integral towards meeting the demanding performance in professional sports arena. In this work, we describe a pilot study carried out to understand the effect of a novel phytonutrient- and electrolyte-fortified sports nutrition composition, PerformLyte^TM, developed in our laboratories, in boosting the performance of trained male cyclists. PerformLyte^TM is a unique fortified formulation comprising of polyphenolic antioxidants, flavonoids, and anthocyanins, as well as carotenoids, sulforaphanes, exotic spices, sea water-derived electrolytes, and vitamin C, encapsulated in proprietary patent-pending Prodosome absorption technology, enabling sustained …

Stayin' Alive (Even Without Oxygen): Retinal Development In Austrofundulus Limnaeus, Carmen Zecilia Rodriguez

Dissertations and Theses

The vertebrate eye is one of the most metabolically active organs in the body and is particularly sensitive to environmental stressors such as oxygen deprivation. Understanding how eye development proceeds under such conditions can provide critical insights into mechanisms of cellular resilience, tissue organization, and developmental plasticity. The annual killifish Austrofundulus limnaeus represents a unique model for investigating these processes due to its remarkable tolerance to anoxia during embryogenesis. However, the wild-type form is heavily pigmented, limiting the effectiveness of traditional imaging techniques for studying eye development. This thesis characterizes eye development and anoxia resilience in both wild-type and melanin-deficient …

Stake-And-Weave Trellising Produces Higher Tomato Yield In High Tunnels Than Does A Double- Or Multiple-Leader Vertical-String Support System, Elizabeth Maynard

Department of Horticulture & Landscape Architecture Faculty Publications

Yield of tomatoes (Solanum lycopersicum) grown in high tunnels in Wanatah, IN, USA, was evaluated using two plant support systems: stake-and-weave or vertical-string. With stake-and-weave, plants were not pruned; stakes were placed every two plants and string was woven horizontally along either side of the plants along the row. With vertical-string, indeterminate cultivars Big Beef and Cherokee Purple were pruned to two stems and each stem was clipped to a vertical string. For the determinate cultivar Mountain Fresh, all branches below the first main stem flower cluster except one were removed and the main stem and major branches …

The Effects Of Tfeα And Promoter Sequence On Early Events In Archaeal Transcription Initiation, Madolyn Toli

Dissertations and Theses

Expression of genes is dependent on promoter sequences in DNA, which direct transcription by RNA polymerase. Transcription factors interact with promoters and RNAP and are often conserved and are essential for proper gene expression. In this thesis, a functional region of archaeal TFEα, homologous to eukaryotic TFIIEα, has been identified, and specific amino acids in these regions have been determined to have significant importance in TFEα's ability to promote transcription in early initiation. All three Domains of life encode a 16S rRNA promoter, which is responsible for production of the 16S portion of ribosome. Transcription with this promoter is not …

Fruquintinib In Less Pretreated Patients: Multivariate Profile-Matching Analysis Of Fresco-2 To Fresco, Arvind Dasari, Cathy Eng, Sara Lonardi, Rocio Garcia-Carbonero, Toshiki Masuishi, Chiara Cremolini, François Ghiringhelli, Joleen Hubbard, Tanios Bekaii-Saab, Jeremy Jones, Rui-Hua Xu, Lin Shen, Jianming Xu, Yuxian Bai, Yanhong Deng, Ying Yuan, Wei Wei, Jianchang Lin, Lucy Chen, Zhao Yang, William R Schelman, Shukui Qin, Jin Li

Faculty, Staff and Student Publications

Background: In the phase 3 FRESCO (NCT02314819) and FRESCO-2 (NCT04322539) studies, fruquintinib vs placebo, plus best supportive care, significantly improved overall survival (OS) in patients with metastatic colorectal cancer (mCRC). These studies were conducted in temporally and geographically diverse populations that received distinct prior therapies; FRESCO patients were less pretreated than FRESCO-2 patients. This analysis assessed the efficacy and safety of fruquintinib in a less pretreated global population than the FRESCO-2 intention-to-treat (ITT) population.

Methods: In FRESCO and FRESCO-2, patients were randomized 2:1 to receive oral fruquintinib 5 mg or matched placebo. Profile matching was undertaken …

A Phase 2 Study Of Obinutuzumab Combined With Lenalidomide In Previously Untreated High Tumor Burden Follicular Lymphoma, Neha Akkad, Lei Feng, Jason R Westin, Fredrick B Hagemeister, Hun Ju Lee, Luis Fayad, Sairah Ahmed, Ranjit Nair, Maria Alma Rodriguez, Paolo Strati, Dai Chihara, Christopher R Flowers, Linda Claret, Karina Ibanez, Michael Wang, Nathan H Fowler, Jared Henderson, R Eric Davis, Sattva S Neelapu, Michael Green, Loretta J Nastoupil

Faculty, Staff and Student Publications

Follicular lymphoma (FL) has a clinical course that is often characterized by high response rates to first-line therapy, followed by multiple relapses over a prolonged natural history. Currently, there are multiple possible approaches to frontline therapy for untreated advanced-stage FL, but there is an ongoing debate around what is the preferred approach. Based on the benefits seen with combining lenalidomide, an immunomodulatory agent, with rituximab, an anti-CD20 antibody, we aimed to evaluate the safety and efficacy of lenalidomide in combination with obinuzutumab, an anti-CD20 antibody with enhanced antibody-dependent cellular cytotoxicity. The eligibility criteria included a diagnosis of FL, grade 1 …

Integrative Techniques To Alleviate Abiotic Stress In Plants Using Plant Growth Promoting Bacteria And Fungi: Mechanisms, Interactions, And Applications, Muhammad Naseem, Amjad Iqbal, Muhammad Qadir, Anwar Hussain

All Works

No abstract provided.

Creating Sustainable School And Home Gardens: Using Citizen Science, Kaitlin U. Campbell, Rita Hagevik, Kathy Cabe Trundle

All Current Publications

Citizen science, also called community-based science or participatory science, provides a way for anyone to gather data, along with scientists, around a scientific question or phenomenon. Citizen science offers immense value to both the general public and scientists. This fact sheet provides resources to connect readers to citizen science projects.