Digital Commons Network^™

C/Ebpα Is Essential For Gonadal But Not Inguinal White Adipose Tissue Formation In Mice, Krista Y. Hu, Yu-Lin Ma, Esme A. Dodge, Olivia A. B. Maguire, Caio V. Matias, Ryan K. Barney, Hector S. Himede, Juliana Gomez Pardo, Miriam Cepeda, Scott M. Gordon, Robert C. Bauer

Physiology Faculty Publications

Objective: The distribution of excess white adipose tissue (WAT) in obesity correlates with risk for comorbidities. Thus, understanding depot-specific WAT developmental mechanisms is translationally relevant. SNPs near the gene CEBPA associate with waist to hip ratio, and while C/EBPα is a recognized regulator of adipogenesis, there is no previously known role for C/EBPα in regulating adipose distribution.

Methods: We crossed Cebpa floxed mice to the AdipoQ-Cre transgenic mouse strain, generating mice with adipocyte-specific knockout of Cebpa (Cebpa_ASKO). Mice were phenotyped on a chow diet and after prolonged high-fat diet (HFD) feeding.

Results: Cebpa_ASKO mice almost entirely lack gonadal …

Dim Light At Night Worsens Cardiac Autonomic Dysregulation In Female Diabetic Mice, Abhilash Prabhat, Shrishti Naidu, Isabel G. Stumpf, Tanya S. Seward, Elizabeth A. Schroder, Brian P. Delisle

Physiology Faculty Publications

Cardiovascular autonomic neuropathy (CAN) is a common complication of diabetes mellitus. CAN is characterized by impaired cardiovascular autonomic control and is associated with increased mortality, especially among women with type 2 diabetes. Identifying environmental factors that exacerbate the CAN-associated phenotype may reveal modifiable strategies to reduce cardiovascular risk.

Nighttime light exposure is a novel cardiovascular risk factor. Among ∼90,000 individuals, greater nighttime light exposure correlated with increased atrial fibrillation and heart failure risk. Light at night disrupts sympathovagal balance and elevates nocturnal heart rate in humans. Dim light at night similarly disrupts autonomic regulation of heart rate in mice. We …

Early Microglial Response To Amyloid Plaques Drives Sleep Loss In Alzheimer’S Disease, Nicholas J. Constantino, Riley E. Irmen, Matthew J. Lanning, Sierra M. Turner, Clair C. Ashley, Caitlin M. Carroll, Evan M. Neary, Dave Rubinow, J. Andy Snipes, Shannon L. Macauley

Physiology Faculty Publications

Introduction: Sleep disruption is an early feature of Alzheimer's disease (AD), but the cellular mechanisms linking amyloid pathology to sleep loss remain unclear.

Methods: Electroencephalography/electromyography (EEG/EMG) recordings, quantitative EEG analysis, and sleep deprivation were performed in APPswe/PSEN1dE9 (APP/PS1) mice at different stages of pathology relative to normal aging. Amyloid burden and microglial density were quantified with whole-brain light-sheet microscopy. CSF1R-mediated microglial depletion explored effects of microglia on sleep loss.

Results: Amyloid plaques caused non–rapid eye movement (NREM) sleep loss that did not worsen with increased plaque burden. Aging reduced REM sleep and eliminated sleep rebound. Amyloid pathology was associated with …

Agricultural Evidence Synthesis At A Critical Juncture: Quality Gaps And Implications For Evidence-Based Agriculture, Chao Cai, Jane Yatcilla, Justyna Czader, Sylvie Brouder, Jeffrey Volenec

Libraries Faculty and Staff Scholarship and Research

Systematic reviews and meta-analyses have become essential tools for synthesizing evidence to guide agricultural policy and practice, yet whether these syntheses are themselves conducted with sufficient rigor has rarely been systematically assessed. This study provides a field-wide assessment of reporting quality in agricultural evidence synthesis, examining whether existing reviews are conducted with sufficient transparency and rigor to support the policy and practice decisions they are meant to inform. We drew a random sample of 244 systematic reviews and meta-analyses from a bibliometric dataset of 1,709 articles published between 1997 and 2023, evaluating reporting quality using a 12-indicator rubric across four …

The Forensic Implications Of Hydrochloric Acid (Hcl) Exposure On Dna Preservation In Human Teeth, Shaelyn Lee Zimmerman

Graduate Student Theses, Dissertations, & Professional Papers

Identity is a human right, and forensic anthropological methods are often aimed at returning identity to decedents. Three main pathways are used for the identification of human remains: fingerprints, dental records, and DNA analysis. Identification efforts may be hindered when perpetrators attempt to obscure the victim’s identity. In cases where corrosive substances, such as hydrochloric acid (HCl) are used, dental comparison and fingerprinting often fail and genetic analysis becomes the best chance of achieving personal identification.

Previous studies have shown teeth are an excellent source of DNA. Large, multi-rooted teeth, such as molars, are often preferred because they contain the …

Labor Savings From Mechanical Rice Transplanting In Bangladesh, India, Nepal, And The Philippines: A Systematic Review And Meta-Analysis, Rodrigo S. David

ASEAN Journal on Science and Technology for Development

Rice production in South and Southeast Asia faces serious challenges, including acute labor shortages and rising wages, which threaten regional food security. Mechanical rice transplanting offers a promising solution; however, evidence on labor savings, especially region-specific data, remains inconsistent. This systematic review and meta-analysis aimed to quantify labor savings from mechanical versus manual transplanting across Bangladesh, India, Nepal, and the Philippines.

Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic search was conducted across four major scientific databases (2000–2026) using Boolean search terms for mechanical transplanting and labor outcomes. Of 284 initial records, 52 met eligibility …

Climate And Seasonal Influences On Indo-Pacific Sea Level: Insights From 30 Years Of Satellite And Tide Gauge Data, Karlina Triana

ASEAN Journal on Science and Technology for Development

We analyze Indo-Pacific sea-level variability using monthly satellite altimetry (1993–2025) validated against records from 16 tide-gauge stations. Cross-comparisons show strong agreement, especially in the western Pacific, confirming the reliability of altimetry for regional assessments. Seasonal SLA variability is largest in the Bay of Bengal and South China Sea and reflects monsoonal forcing, whereas interannual fluctuations in the eastern Indian and western Pacific oceans are dominated by ENSO and modulated by PDO. Harmonic decomposition isolates annual and semi-annual cycles, and an EOF/PCA framework identifies the leading modes: EOF1 (30.8%) captures basin-scale interannual variability and EOF2 (20.9%) reflects the seasonal cycle. Spectral …

Long Biogeographic Separation Limits Arthropod Associations On Eastern Asian Plants Introduced To Eastern North America, Robert J. Warren

Biology Faculty Datasets

No abstract provided.

Bees Carry More Exposed Pollen Than Flies And On Body Regions Well Placed For Floral Contact, Robert Warren

Biology Faculty Datasets

Pollination studies generally emphasize bees, although flies are abundant flower visitors and may remain active under conditions in which bees do not forage. Pollination success depends not only on how much pollen is carried but also if the pollen is carried on body surfaces likely to contact flower stigmas. We compared exposed pollen load and its body placement on 527 bee (Hymenoptera) and fly (Diptera) specimens collected in a commercial apple orchard. Pollen was quantified by direct microscope counts for 376 specimens and hemocytometer wash counts for an additional 151 specimens. Pollen concentrated within scopae and corbiculae, which is less …

Clinical Outcomes With Perioperative Nivolumab By Nodal Status In Patients With Stage Iii Resectable Nsclc: Phase 3 Checkmate 77t Exploratory Analysis, Mariano Provencio, Mark M Awad, Jonathan D Spicer, Annelies Janssens, Fedor Moiseyenko, Yang Gao, Yasutaka Watanabe, Aurelia Alexandru, Florian Guisier, Nikolaj Frost, Fabio Franke, T Jeroen Nicolaas Hiltermann, Jie He, Fumihiro Tanaka, Shun Lu, Cinthya Coronado Erdmann, Padma Sathyanarayana, Phuong Tran, Vipul Devas, Tina Cascone

Faculty, Staff and Student Publications

Individuals with non-small-cell lung cancer (NSCLC) with metastases to the ipsilateral mediastinum or subcarinal lymph nodes (N2 disease) have poor long-term survival. This exploratory analysis from the randomized phase 3 CheckMate 77T study assessed clinical outcomes by nodal status in individuals with stage III NSCLC who received neoadjuvant nivolumab plus chemotherapy followed by surgery and adjuvant nivolumab (nivolumab) versus neoadjuvant chemotherapy followed by surgery and adjuvant placebo (placebo). Here we show that among patients with N2 disease, nivolumab versus placebo improved event-free survival (1-year rate, 70% versus 45%; hazard ratio, 0.46 (95% confidence interval, 0.30–0.70)) and pathological complete response rate …

Synaptic Connectivity Of Rod Bipolar Cells In The Mouse Retina: A Connectomic Analysis Using Webknossos, Harold F. Eshun

Williams Honors College, Honors Research Projects

Rod bipolar cells (RBCs) are key interneurons in the mouse retina that transmit signals from rod photoreceptors to downstream amacrine and ganglion cells under scotopic (low-light) conditions. Although their general morphology and function are well established, the fine-scale organization of their synaptic inputs and outputs remains incompletely characterized. This project aims to map the pre- and postsynaptic partners of a fully reconstructed rod bipolar cell using the Yu volume, a serial block-face electron microscopy dataset from the ventral retina of an adult wild-type mouse. Using the webKnossos platform, I identify synaptic ribbons in the axon terminals of the rod bipolar …

An Investigation Into The Potential Role Of Thioester Bonds In Prebiotic Atp Synthesis, Adam J. Ruf

Williams Honors College, Honors Research Projects

In this project I will attempt to show a potential prebiotic pathway for the synthesis of ATP. This pathway involves the use of thioester bond containing molecules, as derivatives of these bonds have been shown to exhibit enzyme-like catalytic properties. Nuclear magnetic resonance spectroscopy will be utilized to characterize each step of the pathway to ensure it is successful.

Spiders In The World Of Pesticides: A Review, Jackson L. Mendillo

Williams Honors College, Honors Research Projects

Pesticides and other agrochemicals have varying effects on spiders depending on their mechanism of action and dosage, ranging from behavioral changes and ataxia, to reproductive changes and physiological stress, to lethal non-target effects. Additionally, peptide Hv1a has promising effects as a more narrow-spectrum pesticide.

Pipeline Corrosion And Relevant Microbial Influence, Renee E. Bamberger

Williams Honors College, Honors Research Projects

This study investigated the role of microbial activity and oxygen availability in the corrosion of carbon steel pipelines with a focus on microbially influenced corrosion (MIC). Sediment collected from Old Woman Creek, Ohio, was used to simulate natural soil conditions, and carbon steel coupons were incubated under four treatments: nonsterile oxic, nonsterile anoxic, autoclaved oxic, and autoclaved anoxic over 85 days. Corrosion was assessed through mass loss measurements and the analysis of pH, sulfate, chloride, and dissolved iron concentrations. The greatest mass loss was observed in autoclaved oxic conditions, contrary to the initial hypothesis that microbial activity would greatly enhance …

Reconnecting The Body With Nature: Inspired By Advances In Biomimicry, Elyse M. Orosa

Williams Honors College, Honors Research Projects

Reconnecting the Body with Nature: Inspired by Advances in Biomimicry will visually address the symbiotic relationship between nature and humans. The drawing series is inspired by the growing design field of biomimicry and its technological and architectural innovations influenced by nature’s systems and physical adaptations. Just as nature is limited by human misuse, humans are physically and neurologically limited by the state of the environment, and with this, form and function of both nature and humans will be equally represented and explored throughout the body of work presented. Biomimicry can help solve environmental issues such as rising CO2 emissions and …

Effects Of Warmer Springs And Urbanization On The Nest Choice Of Temnothorax Ants, Keana Royce

Williams Honors College, Honors Research Projects

Climate change is altering winter and spring conditions in northeast Ohio, with urbanization and the urban heat island effect likely intensifying these changes. This preliminary study examined how Temnothorax ants alter nest-site preference in response to climate change and urbanization. I hypothesized that colonies exposed to warmed treatments would select hickory nuts to buffer against thermal/urban stressors. Identical in situ experimental setups were deployed at a rural site and an urban site and data collection occurred from late winter to early spring. Control colonies experienced ambient temperatures while experimental colonies were warmed +4°C above ambient temperatures. Colonies had the choice …

C. Difficile Detection Method For First Responder Glove Application, Alli N. Senedak

Williams Honors College, Honors Research Projects

Clostridioides Difficile (C. diff) is a Anaerobic Gram-positive bacillus that is capable of spore formation, making it difficult to control its spread and duration in clinical environments. This phenomenon can provide danger to first responders, healthcare workers, and patients. The goal of this project is to create a biosensor capable of detecting C. diff in a clinical setting that can be applied to a glove apparatus. The project will involve the use of C. diff aptamers activated on the surface of an electrode. Once surface activation has been verified via surface analysis, the electrodes will be exposed to C. diff …

Repurposing Cyclin-Dependent Kinase Inhibitors For The Discovery Of Novel Antiplasmodial Compounds, Ritam Bhowmik

Honors Undergraduate Theses

Malaria remains a major global health burden with over 282 million cases annually and rising resistance to artemisinin-based combination therapies. Plasmodium falciparum, the most lethal malaria parasite species in humans, relies on tightly regulated cell cycle progression during replication within red blood cells. Cyclin-dependent kinases (CDKs) are central regulators of eukaryotic cell division and transcription, and several CDK-like enzymes in P. falciparum, including Pfmrk and PfPK6, are essential for parasite growth and structurally distinct from human kinases. This study evaluated whether commercially available human CDK inhibitors (CDKIs) targeting cancer pathways could be repurposed as novel antimalarial compounds. A library of …

Manuka Honey Modulates Oxidative Stress In Neuro2-A Cells Through Mitochondrial Regulation, Rachel Abramovici

Honors Undergraduate Theses

Manuka honey is known for its vital usage in wound healing, being an agonist towards fighting infections, and is an acting antioxidant [32]. Its main constituents are flavonoids and phenolic compounds, with methyl syringate (MSYR) being a major active ingredient. The specific compounds responsible for these antioxidant effects, particularly in the CNS, remain unclear. It is hypothesized that pre-treatment of mouse neuroblastoma cells with MSYR followed by hydrogen peroxide exposure will activate the AMPK/SIRT1/PGC-1a pathway, due to MSYR’s phenolic structure, and thus reduce oxidative stress and improve mitochondrial biogenesis. Hydrogen peroxide is a standard reactive oxygen species (ROS) treatment to …

Impact Of Lifestyle Factors On Allergic Rhinitis Symptoms And Quality Of Life Among College Students, Lina Saint-Hilaire

Honors Undergraduate Theses

This study aims to examine the influence of certain lifestyle factors on the presentation of allergic rhinitis symptoms and the quality of life of college students. Factors of interest include residential setting, household cleaning habits, age of symptom onset, and exposure to household pets. Additional analysis includes assessment of the impact of allergic rhinitis symptoms on sleep. Using a cross-sectional survey design, student responses were self-reported through a paper survey distributed during tabling days at the University of Central Florida (UCF) main campus. Eligible participants had to be 18 years or older and current UCF graduate and undergraduate students. Survey …

Multicomponent Deoxyribozyme Probes For Targeted Detection Of Cancer Associated Gene Insertions, Samuel R. Veleke

Honors Undergraduate Theses

Insertions and deletions (indels) in DNA sequences of genes are linked with cancers such as glioblastoma (GB). Thus, indels may be used as potential cancer biomarkers. In the NANOG gene, its pseudogene NANOGP8 has a 22 base pair insertion sequence for GB patients. In this work, the NANOG gene and the insertion sequence were detected in one sample using a dual-fluorescent assay containing two multicomponent split deoxyribozyme (Dz) probe complexes. One complex used TXR fluorescence to detect the gene regardless of the insertion’s presence. The other complex used fluorescein (FAM) fluorophore to detect the insertion. The probes were able to …

Investigating The Interaction Of Gram-Positive Lactobacillus Species With Mucin In The Context Of Barrett's Esophagus, Ritisha Suresh

Honors Undergraduate Theses

Gastroesophageal Reflux Disease (GERD) can progress to the precancerous Barrett’s Esophagus (BE) condition, in which the normal esophageal squamous epithelium is replaced with a columnar epithelium containing goblet cells. Mucins, sticky substances, are secreted in response to bile acid exposure during reflux episodes to protect the esophageal epithelium. Goblet cells secrete these mucins, which can also scaffold microbial colonization. Probiotic Lactobacillus bacteria can use glycosidase enzymes to process mucin and metabolize them into their component sugars (glycans). This project aims to identify the role of the Lactobacillus-mucin interaction in BE, as glycan availability could confer an advantage to Lactobacillus …

Histochemical Alteration, Protein Nitration, And Antioxidant Expression In Oysters As Early Warning Indicators For Microplastic Contamination In The Texas Gulf Coast, Rebecca Muñiz, Md Faisal Amin, Md. Saydur Rahman

School of Earth, Environmental, & Marine Sciences Faculty Publications

Microplastics are widely distributed in aquatic environments and have been detected in numerous organisms. Marine bivalves are among the most affected aquatic organisms by microplastics due to their filter-feeding behavior. In this study, 60 oysters were collected from two sites along the Texas Gulf Coast. Fourier transform infrared (FTIR) spectroscopy and Nile red staining were used to detect microplastics in oyster (Crassostrea virginica) tissues. We compared field and laboratory samples collected after a two-week depuration period to identify differences in the shape and size of microplastics in oyster tissues. Results from the field sites showed a large number of microplastics, …

Spatio-Temporal Responses Of A Migratory Shorebird Community Reflect Complex Trade-Offs Among Overabundance Of Predators And Disturbance, K. M. Aarif, Aymen Nefla, Zuzana Musilova, Petr Musil, Sora M. Estrella

Research outputs 2022 to 2026

Animal selection of foraging grounds is a trade-off among available feeding resources, intra and interspecific competition, disturbance and predation risks. The present study was carried out at Kadalundi-Vallikkunnu Community Reserve on the West coast of Kerala (India) in the Central Asian Flyway. The Reserve hosts different coastal habitats, including mudflats, mangroves and sand beaches, and although 31 species of shorebirds are commonly present, it is heavily impacted by human activities. The findings highlight the necessity of robust management of anthropogenic waste and reveal the detrimental impacts of human activities on species communities of conservation concern. We investigated how the densities …

Success Criteria: Sustainable Efficacy In Australian Farmer Groups, David K. Mckenzie, Janine Joyce, Kerstin K. Zander, Penelope A.S. Wurm, Kim M. Caudwell

Research outputs 2022 to 2026

Purpose: To examine the factors and processes that sustain the efficacy of farmer groups as extension intermediaries for influencing collective innovation, and knowledge co-creation and exchange. Design/Methodology/Approach: Data was collected from farmer interviews, focus groups, and the published digital documents of five farmer groups across SE Australia. A grounded theory mixed-methods approach was used for data collection and analysis, aided by Atlas software in constructing concepts and a concept map representing group processes. Findings: The ability of groups to influence farm innovation, and farmer learning and knowledge co-creation, depends on the group efficacy. Group identity in advancing farmer interests and …

High Coral Thermal Tolerance At A Potentially Climate-Resistant Reef, Kate M. Quigley, C. Siqueira, Kira Brereton, Andrew H. Baird

Research outputs 2022 to 2026

Ecosystems globally are under increasing pressure from warming caused by climate change. However, some locations appear resistant to impacts. Although rare, these locations might be able to better resist damage from thermal stress. In marine environments, coral bleaching is widely viewed as one of the most pervasive stress responses threatening reef health, structure, and function. Yet we still have a poor understanding of the drivers of spatial and temporal variation in the scale and severity of coral bleaching. Here, we characterize a prolonged, intense, and widespread marine heatwave event and uncover a conspicuous lack of bleaching at the southernmost coral …

Viral And Host Cell Determinants Of Resistance To Complement-Mediated Killing During Parainfluenza Virus Type 5 Infection, Jenna R. Aquino

Graduate Studies Theses and Dissertations 2026

RNA viruses are a major global public health concern due to high mutation rates, zoonotic potential, and immune evasion. Parainfluenza virus 5 (PIV5) can establish persistent infections (PIs) despite encountering innate immune responses, including the complement (C′) system. RNA virus replication produces defective interfering particles (DIPs) containing truncated defective viral genomes (DVGs) that depend on standard (STD) virus for replication. DVGs are increasingly detected in natural infections and modulate type I interferon (IFN-I), but their role in the C′ system is unclear. We investigated how DVGs affect C′-mediated lysis during PIV5 infection using real-time in vitro cell viability assays. C′ …

Feeding Mode Is Associated With Infant Night Sleep Trajectories During The First Postnatal Year, Magdalena Olson, Li Liu, Elizabeth Reifsnider, Dean V. Coonrod, Sarada S. Panchanathan, Megan E. Petrov, Corrie M. Whisner

Ellmer School of Nursing Faculty Publications

Background: Short sleep and formula feeding during infancy are associated with increased risk of childhood obesity. Feeding practices and sleep arrangements vary during infancy and may also be dynamic, yet their impact on infant night sleep duration remains unclear. Understanding these relationships is crucial for formulating recommendations to support breastfeeding and address sleep concerns. Objective: We examined the association between feeding mode and parent-reported infant night sleep duration during the first postnatal year, while additionally evaluating night-weaning and bedsharing as contextual sleep-related practices. Methods: Infants in the Phoenix Metropolitan Area (n = 193) were followed up at 3, 8, 13, …

Breaking Down Seagrass Fragmentation In A Marine Heatwave Impacted World Heritage Area, Michael D. Taylor, Simone Strydom, Matthew W. Fraser, Ana M.M. Sequeira, Gary A. Kendrick

Research outputs 2022 to 2026

Marine heatwaves, and other extreme climatic events, are driving mass mortality of habitat-forming species and substantial ecological change worldwide. However, habitat fragmentation is rarely considered despite its role in structuring seascapes and potential to exacerbate the negative impacts of habitat loss. Here, we quantify fragmentation of globally significant seagrass meadows within the Shark Bay World Heritage Area before and after an unprecedented marine heatwave impacting the Western Australian coastline over the austral summer of 2010/11. We use a spatial pattern index to quantify seagrass fragmentation from satellite-derived habitat maps (2002, 2010, 2014 and 2016), assess potential predictors of fragmentation and …

Sulphur Metabolism As A Key Factor In The Evolution Of Environmental Adaptation Of Acidihalobacter, Eva Vergara, Himel Nahreen Khaleque, Gonzalo Neira, Elizabeth L.J. Watkin, Jorge H. Valdés, David S. Holmes

Research outputs 2022 to 2026

This study compares predicted sulphur metabolism genes across four Acidihalobacter type strains and two metagenome-assembled genomes (MAGs), revealing genomic differences that appear to correspond to ecological specialization. Phylog-enomic analysis separates the species into two clades: clade I includes Acidihalobacter ferrooxydans from a geothermal region in Italy and the two MAGs derived from deep-sea hydrothermal vents in the Pacific Ocean, while clade II comprises Acidihalo-bacter aeolianus and Acidihalobacter prosperus from a geothermal region in Italy and Acidihalobacter yilgarnensis from a saline and acidic drainage in Australia. Variations in sulphide/quinone oxidoreductases (SQRs) across the species, in particular in Ah. ferrooxydans and Ah. …