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Evaluating Source Material And Seed Propagation Techniques For Spartina Alterniflora To Enhance Salt Marsh Restoration In Coastal Georgia, Maygan Palacios

College of Graduate Studies: Theses & Dissertations

Salt marshes provide critical ecosystem services, including carbon sequestration, flood attenuation, and habitat for diverse aquatic and terrestrial species. Despite their ecological importance, these habitats are increasingly threatened by anthropogenic activities and rising sea levels. To protect and restore salt marshes and their ecosystem services, restoration efforts using living shorelines (LS) have become more common. Along the Eastern and Gulf coasts of the United States, the native grass Spartina alterniflora is commonly used in LS installations for its key role in salt marsh food webs, resilience to abiotic stress, and ability to stabilize and build sediment. Previous studies have documented …

Comparison Of The Biodiversity Of Culturable Yeast From Four Different Habitats, Frank V. Addeo

All Master's Theses

Yeasts are ubiquitous unicellular fungi that play critical roles in decomposition, nutrient cycling, and fermentation processes across diverse ecosystems. Despite their ecological and biotechnological importance, the diversity and distribution of wild yeast populations in many temperate regions remain poorly characterized. This study evaluated how classifier thresholds influence species assignment and functional inference from internal transcribed spacer 2 (ITS2) Sanger sequences of cultured yeasts collected from diverse habitats in central Washington. The project aimed to assess the stability of taxonomic calls across classifier settings and to illustrate how methodological decisions shape downstream ecological conclusions. Samples from each habitat were enriched under …

Low Energy Availability And Dietary Factors In High School Runners, Natalie E. Allik

All Master's Theses

ABSTRACT

LOW ENERGY AVAILABILITY AND DIETARY FACTORS IN HIGH SCHOOL RUNNERS

By

Natalie Elizabeth Allik

June 2026

Context: Low energy availability (LEA) is associated with adverse health and performance outcomes in athletes and may be particularly concerning during adolescence when growth and bone development are ongoing. Although inadequate carbohydrate (CHO) intake has been proposed as a contributor to LEA, limited research has examined this relationship in adolescent endurance athletes. Objective: To examine dietary intake, bone mineral density (BMD), and eating disorder (ED) risk in adolescent distance runners with and without LEA. Methods: Eighteen adolescent distance runners (15.7 ± 1.2 years; …

Exploring Students' Perceptions Of Active Learning Practices And Their Role In Critical Thinking Development In Undergraduate Biology Education, Kwame Kyeremah

All Master's Theses

As the global landscape becomes increasingly complex and undergoes more rapid changes than ever before, critical thinking has become an indispensable skill for addressing challenges in society, science, and the workplace. Despite its widespread adoption in undergraduate STEM education, active learning remains poorly understood from the student's perspective. This study investigated how undergraduate biology students perceived active learning in a ten-week biostatistics course (BIOL 213) at Central Washington University, and whether those perceptions aligned with critical thinking gains measured by the California Critical Thinking Skills Test with Numeracy (CCTST-N). A convergent mixed-methods design integrated student perception and mindset survey data …

Ultrasound-Derived Liver Composition And Metabolic Flexibility Under Exercise Stress, Rhodes Van Houten

All Master's Theses

Metabolic Associated Fatty Liver Disease (MAFLD) is characterized by abnormal liver fat accumulation in conjunction with metabolic syndromes. MAFLD affects 33% of individuals worldwide, though little is known about how increased liver fat impacts physiological responses to exercise. This study aimed to analyze the relationship between liver composition and metabolic flexibility during a graded exercise test.  Body composition was analyzed via dual-energy X-ray absorptiometry. Liver ultrasound was evaluated for echogenicity, where elevated mean gray volume (MGV) indicates abnormal liver tissue. Rates of fat (FATox) and carbohydrate (CHOox) oxidation (g/min), respiratory exchange ratio (RER), and blood lactate concentrations ([LA^-], …

Low Energy Availability And Dietary Factors In High School Runners, Natalie E. Allik

All Master's Theses

Context: Low energy availability (LEA) is associated with adverse health and performance outcomes in athletes and may be particularly concerning during adolescence when growth and bone development are ongoing. Although inadequate carbohydrate (CHO) intake has been proposed as a contributor to LEA, limited research has examined this relationship in adolescent endurance athletes. Objective: To examine dietary intake, bone mineral density (BMD), and eating disorder (ED) risk in adolescent distance runners with and without LEA. Methods: Eighteen adolescent distance runners (15.7 ± 1.2 years; 7 males, 11 females) completed baseline assessments including anthropometrics, dual-energy X-ray absorptiometry (DXA), a 24-hour dietary recall, …

Varying Dna Methylation Patterns In The Florida Sand Skink (Plestiodon Reynoldsi) In Relation To Fire Histories, Victoire Hj Litre

College of Graduate Studies: Theses & Dissertations

Epigenetics is the study of changes in phenotype not caused by changes in DNA sequence. DNA methylation is the most well-studied epigenetic mechanism in an ecological context. Reptiles use DNA methylation to respond to environmental stressors and differences among habitats. The Florida Sand Skink (Plastidome reynoldsi) is federally listed as a threatened species. They are endemic to the threatened, fire-dependent Florida scrub habitat. As they persist in burned habitats, the Florida Sand Skink must be able to rapidly respond to wildfires. To better understand how the Florida Sand Skink adjusts to fire-driven habitat changes, it is important to …

Exploring The Impact Of Adverse Childhood Experiences On Preventive Healthcare Utilization In Rural Georgia, Ryan R. Traylor

College of Graduate Studies: Theses & Dissertations

This study examines the relationship between Adverse Childhood Experiences (ACEs) and preventive healthcare use among adults in Georgia, specifically within rural populations facing structural barriers. Using 2023 Georgia Behavioral Risk Factor Surveillance System (BRFSS) data, the research analyzes associations between ACE exposure, rural residence, and preventive care outcomes, including physical exams and flu vaccinations. Guided by an intersectionally adapted version of Andersen’s Behavioral Model of Health Services Use, the study evaluates how predisposing factors, enabling resources, and perceived barriers relate to healthcare engagement. Logistic regression analyses assess the correlation of ACE exposure, cost barriers, provider access, and sociodemographics with preventive …

The Influence Of Localized Water Quality On Eastern Oysters (Crassostrea Virginica) And Their Internal Microbiome Under Changing Environmental Conditions, Jessie Rivera Lafreniere

College of Graduate Studies: Theses & Dissertations

Oysters are found ubiquitously in estuaries along the Georgia coast, where marsh morphology and large daily tidal fluctuations create dynamic and stressful conditions to which oysters may be locally adapted. Based on water quality data from the Sapelo Island National Estuarine Research Reserve, it is evident that changing climatic conditions are rapidly causing shifts in water quality that may be adversely affecting oyster health, especially as ocean acidification alters the carbonate buffering capacity, increasing the amplitude of daily pH variations. Importantly, the rate of change of conditions are not uniform within estuaries, varying on spatial and temporal scales. The symbiotic …

Cogram: A Computational Pipeline For Genome Assembly And Reconstruction Using Graph Neural Networks, William Coggins

College of Graduate Studies: Theses & Dissertations

Genome assembly — the reconstruction of a complete DNA sequence from short, overlapping reads — remains a fundamental challenge in computational biology. A central difficulty is distinguishing true genomic overlaps from spurious connections arising from repetitive sequences, a task that traditional assemblers address through hand-tuned heuristic rules applied to de Bruijn or overlap graphs. This thesis introduces COGRAM (Coggins–Ramasamy Assembly Method), a genome assembly pipeline that reframes sequence reconstruction as an edge classification task on a k-mer overlap graph, replacing heuristic graph cleaning with a learned model.

COGRAM constructs a directed overlap graph from raw sequencing reads using a k-mer …

The Effects Of Stress-Related Hormones On Excitatory Synapse Formation, Autumn C. Garvey

Honors Undergraduate Theses

Stress profoundly influences brain function through neuromodulatory hormones that regulate synaptic plasticity, yet how temporal patterns of hormone exposure shape excitatory synapse formation remains poorly understood. This study investigates how exposure to stress hormones, norepinephrine and cortisol, affects excitatory synapse development. While existing research primarily compares concentration models, real-world stress occurs in variable patterns that may produce distinct neural outcomes. To address this gap, differentiated Neuro2A neuronal cells are exposed to norepinephrine or cortisol under a continuous treatment paradigm designed to model chronic stress conditions. Following treatment, immunofluorescence imaging is utilized to quantify excitatory synapse formation through analysis of presynaptic …

Neighborhood Deprivation Associated With Impaired Sit-To-Stand Performance In Middle-Aged And Older Adults: A Cross-Sectional Analysis With Clinical Implications, Kenneth Harrison, Silvia Campos-Vargas, Brandon M. Peoples, Keven G. Santamaria-Guzman, David T. Redden, Michael A. Samaan, Jaimie A. Roper

Kinesiology and Health Promotion Faculty Publications

Background: Socioeconomic factors significantly influence health outcomes in older adults, yet their impact on specific aspects of mobility remains unclear. This study investigates the relationship between area-level socioeconomic disadvantage and mobility health in older adults.

Methods: We conducted a cross-sectional study of 110 community-dwelling older adults recruited and collected using voluntary response sampling at eight health fairs across rural Southeast Alabama in 2022–2024 (60 ± 16 years, 80% female). Area-level socioeconomic status was measured using the Area Deprivation Index (ADI), a validated composite measure of neighborhood disadvantage. Mobility was assessed using the Instrumented Timed Up and Go (iTUG) test and …

Horizontal Gene Transfer And Reciprocal Chimerism In Compactonostoc (Nostocales, Cyanobacteriophyta) With Evidence For Two New Species In The Genus., Daniel Al Fatayri

Masters Essays

The genus Nostoc has long been known to be polyphyletic, and recent taxonomic work has segregated several Nostoc-like lineages into new genera, including Compactonostoc. Until now, Compactonostoc shennongjiaensis was the only described species in the genus. In a survey of biological soil crusts from Santa Catalina Island (California, USA), we isolated two Nostoc- like strains that phylogenetically affiliate with Compactonostoc. A polyphasic approach based on morphology, 16S rRNA gene phylogeny, 16S–23S ITS secondary structure, and cloned ribosomal operons revealed that each strain harbors two highly divergent ribosomal operon types. One operon (Type 2) is closely related to C. shennongjiaensis and …

The Depuration Of Microplastic Pollution In Aquacultured Indian River Lagoon Oysters, Lee Nguyen

Honors Undergraduate Theses

Plastics are widely used in consumer goods and industrial manufacturing, and their degradation into microplastics (MP, size < 5 mm) has created an emergent environmental pollutant. Plastics readily enter aquatic environments, where they accumulate additional contaminants and bioaccumulate in organisms. While MP contamination in marine ecosystems is well documented, less is known about MP presence in aquacultured shellfish. Depuration, the process of purging contaminants from shellfish, is conducted in sterile seawater without food, which may cause oyster starvation and death. To date, MP depuration has not been examined in the aquacultured eastern oyster (Crassostrea virginica) in published studies. This study had 3 goals: 1) evaluate the effects of algal feed on oyster survival during depuration, 2) determine the effect of algal feed on depuration effectiveness, and 3) investigate the effect of a ten-day depuration on MP concentration in aquacultured C. virginica soft tissue. MP were quantified in oyster tissues, biodeposits, and in industrial algal feed. Tissues were digested using alkaline solutions, manually examined via microscopy, and analyzed using Fourier-Transform Infrared Spectroscopy (FTIR) to investigate MP polymer …

Noninvasive Monitoring Techniques: The Use Of Scat-Sourced Dna And Camera Traps To Monitor Mammalian Carnivore Species At The Ucf Arboretum, Zachary Montealegre

Honors Undergraduate Theses

The 800-acre University of Central Florida Arboretum Natural Lands functions as a conservation area, recreational space, and research site, making it essential to understand the ecological community it supports. Traditional wildlife monitoring methods such as mark-recapture and blood sampling can be effective but may stress animals or disrupt natural behaviors. To minimize these impacts, this study used non-invasive techniques, specifically scat-based DNA analysis and camera trapping, to assess carnivore species' presence. Two ecologically similar sites within the Arboretum were selected. Suspected carnivore scat was collected twice weekly over two months using both structured and opportunistic sampling. DNA was extracted, amplified, …

Quantitative Assessment Of Roasted Coffee Freshness Over Time Using Multi-Parameter Analysis, Maya Fetzer

Honors Theses

Roasted coffee undergoes continuous chemical and physical degradation after roasting, yet industry freshness standards remain empirically defined rather than grounded in measurable parameters. This study developed a multi-parameter framework for quantifying roasted coffee freshness by integrating gas-phase and solid-phase analysis, backed by human sensory evaluation, across three roast levels — light (City+), medium (Full City), and dark (Vienna) — over a four-month storage period. CO2 off-gassing was monitored continuously for 30 day, gas chromatography-mass spectrometry (GC-MS) tracked volatile compound changes in headspace gas, and Fourier transform infrared-diffuse reflectance spectroscopy (FTIR-DRIFTS) quantified oxidation-associated functional group changes, and microindentation assessed bean stiffness. …

Combining Haptic Feedback With Electromyography To Study Knee Injury In Stationary Cycling, Christopher Kirby

Honors Theses

Patellofemoral Pain Syndrome (PFPS) is the most prevalent overuse injury in cycling, often linked to altered neuromuscular activation patterns of the quadriceps and hamstrings. While sensory feedback has successfully modified cyclists posture, there is a critical lack of evidence-based interventions targeting the underlying muscle activation imbalances associated with chronic knee pain. This study aimed to develop and validate a novel, real-time biofeedback system that utilizes electromyography (EMG) to trigger vibrotactile cues, aiming to shift muscle onset timing earlier in the pedal stroke to mitigate PFPS-related imbalances. A closed-loop system was engineered by integrating a Delsys EMG system with a SageMotion …

A Study Of Adaptive Reuse Of A Decommissioned Golf Course Through A Nature-Based Approach: Woodhaven Golf Course, Fort Wort Texas., Justin B. Watson Mr.

Landscape Architecture Masters & Design Theses

Across the nation, golf courses are shutting down due to a lack of demand. Golf course developments began in the United States in the early 1950s During that era, demand for golf courses and housing was high. As golf’s popularity grew, developers saw golf courses in developments as high value-add amenities (Grooms, 2010). During the late 1990s and early 2000s, developers across the country raced to construct as many golf course communities as they could even as the number of golfers leveled out (Downey,2011).

Decommissioned golf courses across Texas are being underutilized as potential amenities for both the local community …

The Evolution Of California Wildfire Governance Through State Bills, Regulations, And Executive Actions, Cassandra Dawn Blevins

History and Political Science | Senior Theses

California faces an escalating wildfire crisis, driven by climate change, drought, and development in fire-prone areas. Despite decades of scientific research on wildfire behavior and risk reduction, catastrophic fires since 2017 raise a critical question: to what extent have California state laws and policies kept up with the intensifying wildfire crisis? Existing scholarship provides extensive insight into wildfire ecology, land-use planning, fuel management, and community resilience, yet it lacks a comprehensive evaluation of whether California’s legislative, regulatory, and executive actions have adapted in a coordinated and evidence-informed manner. In particular, the literature offers limited analysis of how state laws integrate …

Evaluating The Use Of Precision Technology To Understand Differences Between Ketotic And Non-Ketotic Jersey Cows, Emily Kathryn Palermo

Honors Theses and Capstones

No abstract provided.

Tmem116 Crispr-Mediated Knockout Prevents Proper Lens Regeneration In Iberian-Ribbed Newts, Damien T. Mann

Honors Theses and Capstones

Eye diseases like cataracts, age-related macular degeneration (AMD), and glaucoma are widespread and detrimental conditions affecting many people across the globe. While there are reliable and successful treatments for those with cataracts that can reverse vision impediments, others like glaucoma and AMD have limited treatment options. Additionally, eye damage resulting from these conditions is commonly irreversible, establishing a need for new treatment options. The Iberian-ribbed newt, Pleurodeles waltl, is a salamander species primarily studied for their amazing regenerative capabilities. Particularly, these newts are capable of de novo lens regeneration after a complete resection, providing a unique opportunity to study regeneration …

A Course Correction? The Correlation Between Gfsi-Recognized Certifications And Salmonella In Raw Chicken Products, Kar Ho Lim, Lijiao Hu, Yuqing Zheng, Michael Ollinger

Agricultural Economics Faculty Publications

The Global Food Safety Initiative (GFSI) is a widely recognized entity. Many third-party certifications, such as Safe Quality Food (SQF), Brand Reputation through Compliance Global Standard (BRCGS), and Food Safety System Certification 22000 (FSSC), benchmarked against GFSI standards. This study compares the Salmonella detection rates in raw chicken products from SQF, BRCGS, FSSC-certified establishments to those from uncertified ones. We performed a probit regression and calculated average marginal effects using testing data from the Food Safety Inspection Service. Overall, products from certified establishments are associated with 3.2 % lower positive rate of Salmonella detection. Certification is associated with a greater …

Anuran Occupancy Of Created Wetlands Within Reforested Legacy Surface Mines In Kentucky And West Virginia, Jillian C. Newman, Steve Price, Jacquelyn C. Guzy, Joshua Castle, John J. Cox, Jeffery L. Larkin, Christopher D. Barton

Forestry and Natural Resources Faculty Publications

Restoration of habitat is an important component of wildlife management. Surface coal mining has altered forest cover in the Central Appalachians, and most reclamation activities on previously mined lands result in non-native grasslands or shrub lands. The Forestry Reclamation Approach (FRA), which emphasizes non-native vegetation removal, soil decompaction, planting of native trees and shrubs, and, in some cases, wetland creation represents an alternative to grassland-based reclamation that may promote forest-associated wildlife on legacy surface mines. We used automated recording units to evaluate the response of anurans to created wetlands and the FRA in Kentucky and West Virginia, USA. We used …

Microglial Apoe Across Alzheimer’S Disease-Relevant Pathologies, Georgia Nolt

Theses and Dissertations--Physiology

APOE exists as three common alleles (APOE2, APOE3, APOE4) that encode their corresponding protein isoforms (apoE2, apoE3, apoE4). Despite differing by only one or two single-amino acid substitutions, each APOE allele confers a distinct AD risk profile with APOE4 increasing risk by 28% and APOE2 decreasing risk by 14% compared to the most commonly expressed APOE3 allele. While traditionally considered “neutral,” APOE3 still accounts for ~36% of AD cases while APOE4 contributes ~57%, indicating that the majority of AD cases would not occur in the absence of the APOE3 and APOE4 alleles. Microglia, the resident phagocytic immune cells …

Characterizing Gut Microbial Communities And Symbiont-Mediated Detoxification In Herbivorous Insects, Rachel C. Vargas

Biology Dissertations

Insect gut bacteria are vital contributors to host physiology, yet the broader prevalence and fitness implications of symbiont-mediated detoxification remain poorly understood. This thesis investigates these dynamics across three research chapters. Chapter 1 uses 16S rRNA sequencing to characterize how grasshopper gut microbial communities vary across species, dietary breadths, and gastrointestinal segments. Chapter 2 evaluates the detoxification capacity of over 150 bacterial isolates from diverse herbivores against the plant allelochemicals phenethyl isothiocyanate, rutin, and chlorogenic acid to determine if host dietary specialization predicts microbial metabolic ability. Finally, Chapter 3 employs fitness bioassays to test whether inoculating generalist herbivores with detoxifying …

American Elderberry's Next Top Pollinator: Evaluating The Role Of Arthropods In Pollination, Jenny K. Burrow

Graduate Theses/Dissertations

American elderberry (Sambucus nigra subsp. canadensis) is a woody deciduous shrub native to eastern North America. In addition to its long history of use by indigenous peoples, there is renewed interest in cultivating and consuming American elderberry, primarily driven by expanding health supplement markets. Despite recent advances in horticultural development of American elderberry, its pollination ecology remains poorly understood. In this field study, I evaluated the contribution of arthropod visitors to pollination of American elderberry. To do this, I first characterized the identities and abundances of arthropod visitors via timed arthropod collections and floral observations during peak flowering …

Human Plasma Extracellular Vesicles As An Exercise Mimetic To Preserve Skeletal Muscle Plasticity During Disuse, Alexander M. Fliflet, Yanqi Tan, Takeshi M. Barnes, Ane Nishitha Vijayan, Sung Jun Choi

Kinesiology & Sport Management Scholarship

The purpose of this study was to determine the extent to which extracellular vesicles (EVs) circulating in blood after exercise training act as an effective mimetic to maintain skeletal muscle mass during unloading and/or accelerate recovery after disuse. Ten healthy males (27.7 ± 7.1 y) were recruited for a 6-week progressive resistance and endurance training program. EVs were isolated from blood before (EVs) or immediately after training (ExerVs). EVs were intraperitoneally injected into male mice (4×; 3 × 108 particles/injection) during 14 days of hindlimb unloading (HU), then the muscles were collected immediately or 7 days after HU. ExerVs did …

A Transdisciplinary Approach To Advancing Water Security And Public Health: The “Nurse + Engineer” Model For Achieving Sustainability In Wash Systems Using Community-Based Participatory Research, Daniel B. Oerther, Sarah Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Human health is linked to the health of the environment, a concept referred to as planetary health. As environmental challenges become increasingly complex, single-disciplinary approaches are insufficient to achieve sustainable solutions. This article presents a transdisciplinary framework, termed the "Nurse + Engineer" model, as a novel methodology for addressing complex problems at the nexus of environmental engineering and public health. The model integrates the technical design and systems-level thinking of environmental engineering with the community-based, patient-centered care of nursing. This work details the key lessons learned from the retrospective application of this framework to a series of multi-year, community-based participatory …

Environmental Engineers Develop Solutions To Problems Of Planetary Health: The Legacy Of Professor P. Aarne Vesilind, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

The principal ethical obligation of the engineering profession, to "hold paramount the health, safety, and welfare of the public," has been challenged for decades. Professor P. Aarne Vesilind identified two primary deficiencies: an anthropocentric bias that values nature only instrumentally and the use of non-mandatory, aspirational language for environmental protection, which resulted in the exclusion of enforceable environmental canons. This paper argues that two recent developments provide a comprehensive response to Vesilind's recommendations for engineering ethics. First, the United States Bureau of Labor Statistics has redefined environmental engineering as "developing solutions to problems of planetary health." Second, Oerther proposed a …

How Effective Is Online Teaching Of Physiology Modules? Perception Of Rwandan Health Sciences Students, Abdullateef Isiaka Alagbonsi, Mojeed Akorede Gbadamosi, Mathieu Nemerimana, Makinde V. Olubiyi, Olufunke Onaadepo, Akaninyene Ubong Ime, Abdul-Rahuf Aderemi Feyitimi, Ruth Wanjiru Mungai, Florent Rutagarama, Simiat Elias

Biomedical Sciences, East Africa

Background: While blended teaching is approved in Rwandan medical education, the perceived effectiveness of online-only delivery for physiology modules remains unclear. This uncertainty limits its continued use. The present study assessed Rwandan health sciences students’ perceptions of the effectiveness of online teaching for physiology modules.

Methods: A total of 528 students from medicine and surgery (n = 300), pharmacy (n = 99), dentistry (n = 89), and clinical psychology (n = 40) programs at the University of Rwanda completed an online questionnaire. Data were analyzed using multivariable logistic regression and non-parametric tests (Mann-Whitney U, Kruskal-Wallis) with corresponding effect …