Digital Commons Network^™

Ankrd2 May Alleviate Denervation-Induced Skeletal Muscle Atrophy By Inhibiting Inflammation, Yalin Zhang, Lin Zhang, Li Li, Liqin Zhang, Li Zeng, Jiaying Qiu, Zhengrong Xi, Wenwen Wang

Turkish Journal of Biology

Background/aim: Sciatic nerve injury causes a loss of skeletal muscle innervation, reduced motor function, and eventual muscle atrophy. Inflammation and increased protein degradation are key factors contributing to muscle atrophy. Inflammation is activated early during muscle atrophy and can be modulated by various factors. However, the precise role of inflammation in denervation-induced muscle atrophy remains unclear. Materials and methods: Transcriptome sequencing was used to determine that the inflammatory response occurs early during denervation-induced muscle atrophy. RT-qPCR validation of several inflammatory factors showed rapid upregulation at early stages, followed by gradual downregulation. Weighted gene coexpression network analysis of differentially expressed genes …

Unusual Rna Polymerase Ii Patterns In Meiotic Prophase I In Nannospalax Xanthodon: Insights Into Chromatin, Telomeres, And Sex Chromosomes, Sergey N. Matveevsky, Ferhat Matur, Faruk Çolak, Andrei Kandaurov, Mustafa Sözen, Svetlana V. Pavlova, Oxana Kolomiets

Turkish Journal of Biology

Background: Meiotic transcription is a highly regulated process involving stage-specific chromatin remodeling and dynamic RNA polymerase II (RNAPII) activity. Nevertheless, our current understanding is largely based on classical model organisms, while many nonmodel mammals remain unexplored. This study investigates the transcriptional dynamics and chromatin features during meiotic prophase I in Nannospalax xanthodon, a subterranean blind mole rat species with extensive intraspecific karyotype variation.Materials and methods: Using immunofluorescence, we analyzed synapsis and transcriptional activity in spermatocyte nuclei of two geographically distant N. xanthodon males, detecting SYCP3, CREST, γH2AFX, and phosphorylated RNAPII (Ser5, Ser2). Telomereassociated proteins RAP1 and TERT were also examined …

Protective Effect Of Pinocembrin On Zearalenone-Induced Hepatotoxicity Via Pi3k/Akt And Nrf2 Signaling In Albino Mice, Sujatha Tejavat

Turkish Journal of Biology

Background/aim: Zearalenone (ZEA) is a Fusarium-derived mycotoxin that frequently contaminates food and feed and induces hepatotoxicity in humans and animals through oxidative stress and inflammation. Pinocembrin (PCM), a naturally occurring flavonoid, has potent antioxidant and antiinflammatory properties. This study investigated the hepatoprotective effects of PCM against ZEAinduced liver injury in albino mice, with a focus on the nuclear factor erythroid 2–related factor 2 (NRF2) and phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathways. Materials and methods: Twenty-four albino male mice were allocated into four groups: (1) control (vehicle 0.05% dimethyl sulfoxide), (2) ZEA-treated (40 mg/kg body weight), (3) PCM-treated (50 mg/kg …

Improving Reclamation Seeding Success Through Seed Technologies, Surface Preparation, And Microbial Inoculants, Josh M. De Santiago Lazalde

Theses and Dissertations

Revegetation of reclaimed lands is often limited by poor seed placement, unfavorable surface conditions, and reduced biological activity, particularly on steep or physically degraded substrates where broadcast seeding is commonly used. Improving early seeding establishment under these conditions requires approaches that address both physical and biological barriers to seedling development. We evaluated the effectiveness of surface preparation, seed technologies, and microbial inoculants for improving plant establishment in field and greenhouse settings relevant to mineland reclamation. At the field scale (Chapter 1), we investigated the effects of chain harrowing and seed enhancement treatments on the establishment of grasses, forbs, and shrubs …

Impacts Of Arbuscular Mycorrhizal Fungi And Novel Soil Amendments On Perennial Grass Establishment In Mineland Soils, Melissa M. Burrell

Theses and Dissertations

Establishing perennial vegetation on mine waste materials is one of the primary challenges in mine land reclamation. Waste rock and mine tailing substrates typically lack the physical structure, nutrient availability, and biological communities necessary to support plant establishment and long-term ecosystem recovery. As a result, revegetation efforts often experience poor seeding success and limited plant persistence despite intensive management efforts. These studies investigated two complementary strategies to improve perennial grass establishment in degraded mine substrates: restoring mycorrhizal associations and enhancing soil function through soil amendments. The first study examined the role of arbuscular mycorrhizal fungi (AMF) in facilitating plant establishment …

On The Transport Of Chloroplast Lipids, Cailin Smith

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Chloroplasts are green, photosynthetically active organelles ubiquitous to the plant kingdom. Owing to their cyanobacterial origin, chloroplasts consist of three membranes: the outer envelope, the inner envelope, and the thylakoid, the latter of which is the site of the light-dependent reactions of photosynthesis. Not only do the lipids that make up these membranes delineate aqueous compartments, but they also respond dynamically to developmental and environmental cues, constitute the matrix that scaffolds protein machinery, and even serve as functional components of some proteinaceous complexes. However, while lipids are of vital importance to the structure and function of the chloroplast, the mechanisms …

Analysis Of Agricultural Remote Sensing Data Driven By Artificial Intelligence On Crop Growth Patterns And Economic Benefits Of Grain, Yang Wang

Turkish Journal of Agriculture and Forestry

To address the shortcomings of conventional agricultural statistical and monitoring methods on a regional scale, this paper proposes the use of artificial intelligence-driven remote sensing data for analysing crop growth patterns and the economic benefits of grain. Winter wheat data from three stations in a single province from 2011 to 2020 was used, with the leaf area index (LAI) used as the key crop growth indicator value. The simulated annealing algorithm was used to assimilate the LAI and remote sensing leaf area index (MODIS-LAI), simulated by the World Food Study (WOFOST) simulation model, to carry out simulation analysis of winter …

​Spectrophotometric Detection Of Food Additives And Pesticides By Magnetite Cross-Linked Tyrosinase Aggregates (Mcltas)​, İlker Polatoğlu

Turkish Journal of Agriculture and Forestry

In this study, tyrosinase enzyme was cross-linked with glutaraldehyde to form aggregates on the surface of activated magnetite nanoparticles, without the need for a separate support matrix. For the first time, this novel aggregate was employed for the spectroscopic detection of ascorbic acid (AA) and diazinon, as model food additives and pesticides, respectively. This technique, developed as an alternative to optical sensors, not only eliminates their drawbacks but also facilitates the recovery of cross-linked enzyme aggregates (CLEA) from the reaction medium using the incorporated magnetite nanoparticles. A spectrophotometric technique was used to measure the enzyme activity of the aggregates to …

Nitrogen Transport Patterns And Vegetation Community Optimization In Urban Green Space Soils Using Multifactor Coupling Analysis, Shujian Yang, Baoxi Yu, Zheng He, Baochang Liu, Chang Liu

Turkish Journal of Agriculture and Forestry

As vital components of urban ecosystems, urban green spaces directly affect soil fertility and plant growth through nitrogen transport dynamics. This study addresses the existing methodological gaps in understanding nitrogen cycling within urban green spaces, particularly the unclear interaction mechanisms between vegetation structure and soil physicochemical characteristics. The study was conducted in the Green Expo Park located in Nanjing, China. Using a stratified sampling approach, physicochemical indicators of soil layers were measured and combined with community parameters—including leaf area index, vegetation coverage, and plant density—to identify key drivers through correlation and multiple regression analyses. The regulatory mechanisms of soil physicochemical …

Mitigation Of Salinity Stress In Carnation Plants Through Zinc Oxide Nanoparticles: Growth, Physiological, And Biochemical Responses, Adel A. Rezk, Ishtiaq Ahmad, Maryam, Muhammad Saleem, Othman Al-Dossary, Hossam S. El-Beltagi, Mohammed Aldaej, Bader Y. Alsubaie, Hati̇ce Gülen, Tarek Shalaby, Riaz Ahmad

Turkish Journal of Agriculture and Forestry

Carnation (Dianthus caryophyllus L.) growth and floral yield are severely reduced when grown in soils with excessive salinity. Mitigation of adverse effects from salinity-induced stress is necessary for sustainable carnation cultivation. Although various approaches have been developed, the use of zinc oxide nanoparticles (ZnO-NPs) to ameliorate these effects remains scarcely explored. The current study explored the potential of ZnO-NPs to alleviate salinity-induced stress by examining their effects on morphophysiological and biochemical traits. A controlled pot experiment was conducted using a factorial design with three concentrations of sodium chloride (0, 50, and 100 mM) and three of ZnO-NPs (0, 50, and …

Development Of A Grease Resistant Barrier For Food Industry Applications, Mira Marino

Honors Theses

The objective of this project is to develop a grease-resistant barrier coating for paper-based food packaging and to evaluate the economic feasibility of implementing this coating system at an industrial scale. As demand increases, this project focuses on identifying an optimal pigment and binder combination that enhances barrier performance while maintaining cost-effectiveness. The primary goal was to maximize grease and moisture resistance of coated paper while ensuring the process is economically viable and compatible with existing paper mill operations.

The final plant design consists of one continuous stirred tank reactor (CSTR) and three centrifugal pumps integrated into an existing paper …

Investigating The Cost And Efficiency Of Two Chemical Pathways To Produce The Plant-Based Coagulants, Taylor Nixon

Honors Theses

Plant-based coagulants are promising sustainable alternatives for water treatment due to their biodegradability, low toxicity, and reduced risk of secondary pollution. Unlike conventional inorganic coagulants, they generate less sludge and avoid secondary contamination in the environment. This project examines two chemical modification strategies for producing cationic cellulose coagulants. The first involves direct amination of cellulose using CHPTAC, while the second employs periodate-oxidized dialdehyde cellulose, which enables reductive amination through reactive aldehyde groups. These approaches are expected to yield materials with differing charges, surface functionalities, and structural properties that may influence coagulation performance. The study evaluates the technical and economic feasibility …

Optimizing Itpa R178c Assays Via Hplc, Jared J. Reisnouer, Wally D. Pines

2026 Symposium

Two of the major nucleotide bases in DNA and RNA (Adenine and Guanine) are derived from the purine Inosine Monophosphate (IMP). IMP may occasionally form the noncanonical nucleotide Inosine Triphosphate (ITP) within the cell and become incorporated into DNA during replication, leading to potentially lethal errors. To combat this, human cells produce the “housekeeping” enzyme Inosine Triphosphatase (ITPA) to revert ITP to IMP. A mutation of this protein that replaces the 178th amino acid Arginine with Cysteine (R178C) is associated with a fatal infantile encephalopathy. Previous assessments of enzyme-substrate binding and catalysis for ITPA variants have been run at a …

New Ways To Address The Plastic Problem, Andrew J. Hanna

The Penguin Review

Since 1950, the world has produced some 9.2 billion metric tonnes of plastic.1 Other sources place the total closer to ten billion metric tonnes Furthermore, nearly 500 million tonnes of new plastic are manufactured each year– a figure that's projected to increase to 1.2 billion tonnes by 2060.2 The environmental effects of all this plastic production and pollution are disturbing. Because most plastics are synthetic–meaning they contain "no molecules found in nature"– they can't decompose. Instead, they just break down into smaller and smaller pieces, becoming microplastics (less than five millimeters (5 million nanometers) across) and then nanoplastics (less than …

Assessment Of Total Cellular Protein Following Fatty Acid Treatment Using The Bca Assay, Carli B. Maguire

Create@State

Fatty acids can influence cellular metabolism and protein homeostasis, potentially altering total protein content within cell samples. Accurate quantification of total protein concentration is essential for standardization for further biochemical assays and ensuring reproducibility. In this study, adherent mammalian cells were treated with a defined fatty acid preparation under controlled culture conditions. Following treatment, whole-cell lysates were collected and total protein concentration was determined using the bicinchoninic acid (BCA) assay. The BCA assay is a colorimetric method based on the reduction of Cu²⁺ to Cu¹⁺ by peptide bonds under basic conditions, followed by formation of a purple-colored complex between bicinchoninic …

Comparison Of Levels Of Bmi, Stress, And Depression Of Women During The Prenatal Period Relative To Their Activity Levels, Kalayna King

Create@State

This study aimed to analyze the relationships between activity levels, Body Mass Index (BMI), stress levels, and depression symptoms in obese pregnant women in Arkansas. Two separate exercise intervention studies were combined in this analysis to ultimately give a sample size of 293 pregnant women who were randomized into standard of care group (control) and an exercise group (experimental) (n = 115 and n = 178, respectively). Women in the control groups were instructed to independently continue to follow the physical activity guidelines recommended by their gynecologist, whereas the experimental groups participated in a combination of aerobic and resistance exercise …

Skin Pattern As A Potential Monitoring Tool For Frog Populations, Lídize Sagarnaga Rivera, Virginie Rolland Dr., Lorin Neuman-Lee Dr.

Create@State

Skin pattern as a potential monitoring tool for frog populations

Population monitoring, which requires identifying individuals, is crucial for species conservation. Common techniques involve marking, which can harm animals and alter their behaviors. Natural markings are an alternative not yet explored for amphibians. Yet, the traditional method with elastomers (fluorescent implants) is invasive and not completely reliable—the tag may become lost or hard to see. The American green tree frog (Dryophytes cinereus) possesses naturally occurring and distinct golden dots on their dorsal side.  Therefore, we aim to assess whether patterns of golden dots on the skin of green …

Who Will Grow Our Food In The Future? Factors Influencing A-State Agricultural Student’S Intentions To Enter Production Agriculture, Kamana Kafle

Create@State

The future of agriculture depends on what motivates today’s student to pursue careers in production agriculture. This study identifies key drivers of career intention among College of Agricultural students at A-State using an extended Theory of Planned Behavior framework. Data were collected through a cross-sectional survey and analyzed using Partial Least Squares Structural Equation Modeling (PLS-SEM). The model explained a substantial 74.2% of the variance in agricultural career intention. Results revealed that Career Planning was the strongest positive predictor, followed by Attitude Towards Agriculture and Perceived Value. Interestingly, factors such as Perceived Behavioral Control, Subjective Norms, and access to Facilities …

Amyloid Beta-42 Neurotoxicity In Drosophila Retinal Development, Shay O. Sims, Pierce Duncan, Malachi Abon, Jacob G. Smith

Create@State

Alzheimer's disease (AD) represents one of the most prevalent neurodegenerative disorders globally. Thus far, the underlying mechanisms associated with the pathogenesis of AD are innately elusive. Two hallmarks associated with the progression of AD exist, including Amyloid-Beta 42 (Aβ42) protein plaque formation and hyperphosphorylation of tau proteins in neurons. Amyloid-beta is known to concentrate with other neuronal proteins as complexes form, including the cytoskeletal protein actin. However, whether actin contributes to neuronal decline in AD is unknown. To further understand the cellular cascades associated with AD, we investigated the role of cytoskeleton dynamics in modulating oxidative stress and neuroinflammation within …

Transgenic Plant Cells For The Cost-Effective Production Of Pharmaceutical Proteins, Maria Gambaro

Create@State

Plant-based systems are becoming an important method for producing pharmaceutical proteins because they can be more cost-effective, and safer compared to traditional production methods which commonly use mammalian cells. This project aims to develop a transgenic plant cell system that can produce a pharmaceutical protein using molecular cloning techniques. A transgenic plant cell has the DNA of another plant integrated into its genome, altering its gene expression to produce different proteins. To begin, a plasmid carrying the gene of interest and the DNA components needed for expression will be designed. The plasmid will then be used to introduce the gene …

Variation In Infection Prevalence Of Splendidofilaria Pectoralis Across Alaskan Grouse Populations, Anahi Garcia Valdez, Diego Martinez

Create@State

In recent years, rapid climate change has altered species distributions worldwide and influenced parasite transmission dynamics in wildlife systems. Parasite infections play an important role in shaping patterns of disease distribution across species and populations. Understanding variation in infection prevalence across geographic regions and host demographic groups is essential for interpreting these patterns. Splendidofilaria pectoralis is a filarial nematode that infects the connective tissues of grouse (Aves: Tetraonini) in Alaska and has been moving northward as the climate warms. However, relatively little is known about the variation in prevalence of the nematode across geography and demography. This project examines variation …

Relish As A Temporal Switch In Neurodegeneration Using Drosophila Melanogaster, Kyle M. Johnson

Create@State

Neurodegenerative diseases are often linked to chronic inflammation in the nervous system, but it is still unclear what triggers this process during the early stages of disease. This project examines whether activation of the NF-κB transcription factor Relish may act as a temporal switch that contributes to early neurodegenerative stress in Drosophila melanogaster. As a result of many innate immune pathways are consistent between flies and humans, Drosophila serves as a useful model for studying how immune signaling affects neuronal health. In this study, neural driver lines were crossed with control strains to evaluate how changes in Relish signaling …

An Investigation Into Relish Activation As A Temporal Switch In Neurodegeneration, Kyle M. Johnson

Create@State

Neurodegenerative diseases are often linked to chronic inflammation in the nervous system, but it is still unclear what triggers this process during the early stages of disease. This project examines whether activation of the NF-κB transcription factor Relish may act as a temporal switch that contributes to early neurodegenerative stress in Drosophila melanogaster. As a result of many innate immune pathways are consistent between flies and humans, Drosophila serves as a useful model for studying how immune signaling affects neuronal health. In this study, neural driver lines were crossed with control strains to evaluate how changes in Relish signaling …

Inc3vits Model: A Hybrid Architecture To Accelerate And Reduce Complexity For The Deepvariant Model For Variant Calling, Mustafa Al-Saffar, Sura Z. Al Rashid

Karbala International Journal of Modern Science

Deep learning has revolutionized genomic variant calling, yet the computational cost of current systems continues to limit scalability. We present a controlled efficiency study of DeepVariant-style pileup architectures under identical training and inference conditions, comparing architectural downsizing with a hybrid CNN–local attention design. Inc3ViTs pairs a streamlined InceptionV3 stem with a lightweight local attention head based on patch tokenization and windowed self-attention, enabling a direct comparison with a CNN-only reduced Inception baseline. Across whole-genome and whole-exome short-read datasets, Inc3ViTs reduces training time by ~40–50% and reduces inference runtime relative to the original DeepVariant. The CNN-only baseline indicates that most speedups …

Infertility And Mental Health, Laynie Pugmire

Family Perspectives

No abstract provided.

Impacts Of Adoption On Attachment, Laynie Pugmire

Family Perspectives

No abstract provided.

Fatherhood Redefined: How Paternity Leave Shapes Relationships, Olivia Flores

Family Perspectives

Paternity leave remains limited in the United States, with relatively few fathers granted paid time off following the birth of a child. This literature review examines how paternity leave shapes marital relationships by influencing parental mental health and couple communication during the transition to parenthood. Drawing on interdisciplinary research, the paper synthesizes evidence indicating that fathers’ use of paternity leave is associated with higher marital satisfaction and greater relationship stability. These benefits are largely mediated by improvements in both maternal and paternal mental health, as paternity leave reduces role overload, stress, and symptoms of anxiety and depression for both partners. …

Academic And Mental Health Outcomes In Homeschoolers, Emily Higham

Family Perspectives

Homeschooling—education conducted outside traditional school settings and typically led by parents—has grown substantially in the United States, particularly following the COVID-19 pandemic. As participation has increased, greater attention has been directed toward understanding its implications for children’s development. This literature review examines associations between homeschooling, mental health, and academic outcomes. Existing research suggests that homeschooled children generally demonstrate positive socioemotional functioning, including lower levels of internalizing behaviors such as anxiety and depression and fewer externalizing behaviors such as aggression compared to traditionally schooled peers. These outcomes may be supported by increased parental involvement, stronger family relationships, reduced exposure to bullying, …

The World Needs More Resilient Kids, Kara Driggs

Family Perspectives

No abstract provided.

The Effect Of Singlehood On Socioemotional Health, Alexa Garner

Family Perspectives

No abstract provided.