Digital Commons Network^™

P300/Cbp Inhibition With Inobrodib In Combination With Gilteritinib And Venetoclax Targets Leukemia Stem Cells In Epigenetic Mutant Aml, Melanie L. Goetz, Jennifer S. Romer-Seibert, Amanda M. Versace, Scott Kogan, Chetan Jeurkar, Robert L. Bowman, Nigel Brooks, Kris Frese, Sara E. Meyer

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Acute myeloid leukemia (AML) is a fatal blood cancer with cytotoxic chemotherapy offering at best 25% 5-year survival. While targeted BCL2 and FLT3 inhibitors venetoclax and gilteritinib are used upfront in the treatment of a subset of adult patients with AML and help to extend the survival of some patients, a curative treatment combination with minimal side effects has yet to be discovered. We find that use of the dual histone acetyltransferase p300/CBP bromodomain inhibitor CCS1477 (inobrodib), together with venetoclax and gilteritinib, virtually eliminates leukemia stem cells in an aggressive preclinical model of DNMT3A/FLT3-mutant AML by impairing pro-oncogenic survival and …

Associations Between Father–Mother Food Parenting Practices And Children’S Dietary Intake, Brian K. Lo, Natalie Grafft, Mariane Helen De Oliveira, Sebastien Haneuse, In Young Park, Alejandra Cantu-Aldana, Katherine W. Bauer, Rebekah Levine Coley, Jorge E. Chavarro, Kirsten K. Davison, Jess Haines

Human Genetics Publications

Objective: To examine whether the number of parents using high levels of structure or coercive control food parenting practices is associated with children’s dietary intake.

Design: Cross-sectional analysis of father–mother dyads from the Fathers & Families cohort study. Fathers and mothers independently reported their food parenting practices and child’s dietary intake. Multivariate logistic regression models estimated associations between the number of parents (0, 1 or 2) using high structure or coercive control practices and children’s intake of fruit, vegetables, fast food and sugar-sweetened beverages (SSB), adjusting for demographics and recruitment site.

Setting: United States

Participants: 474 father–mother dyads with a …

Cortical Bone Density And Thickness Assessment Of Intraradicular Sites In Adolescent Patients Using Cbct Imaging, Sara Endo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: The indications for the use of cone beam computed tomography (CBCT) in orthodontics has grown since it was first introduced and dentists are discovering new ways that 3D images enhance diagnosis and treatment planning. Evaluating bone quality and quantity can be measured as bone density and thickness in CBCT imaging and is helpful for temporary anchorage device (TAD) placement in orthodontic treatment. TADs rely on primary stability and are commonly used in orthodontic treatment to increase anchorage and expand the limit that nonsurgical orthodontics can provide.

Objectives: This study aims to assess the bone thickness and density at different …

Thermal Physiological Ecology Of The Relict Leopard Frog At Hot And Cold Springs, Robert P. Pelletier Iii

UNLV Theses, Dissertations, Professional Papers, and Capstones

The relict leopard frog (Rana onca) once ranged across drainages in southern Nevada, northwestern Arizona, and southwestern Utah. Following a decline, the species only persisted in a few geothermally influenced hot springs, which led to the perspective that hot springs were high-quality habitat. Rana onca has been under intensive, multiagency management and the species has been translocated to establish additional populations, including at cold-water sites. Three research studies are presented into the thermal physiological ecology of R. onca with the aim of informing conservation strategy. The research was focused at a thermally influenced hot spring and a cold-water spring to …

Ecological And Nutritional Drivers Of Floral Fidelity In Wild Pollinators, Adelaide Elise Ergastolo

Master's Theses

Plants must receive pollen from the same species in order to successfully reproduce. For most flowering plants, the transfer of pollen occurs via animal pollinators while they forage on plants to obtain food. Therefore, the fidelity of pollinators to individual plant species during foraging trips is directly related to the amount of conspecific pollen plants receive. Low floral fidelity of pollinators can reduce plant reproductive success, which in turn has the potential to lower food resources for pollinators in the future. While many factors can potentially influence pollinator floral fidelity, several important ones may be the nutritional resources provided by …

The Development And Evaluation Of An Automated Tactical Tillage Tool To Identify And Control Weeds In Row-Crop Production Systems Using Vision-Based Machine Learning., Clara Anne Cobb

Theses and Dissertations

Controlling in-field weeds in row-crop production systems is vital to the success of the operation. To control weeds, most producers use combinations of weed control strategies, including tillage and herbicide applications. Cotton production operations across the country have been trending toward conservation tillage practices in an effort to minimize soil disruption and maintain crop residues on the soil surface. This practice, in addition to the adoption of herbicide-tolerant cotton varieties, has encouraged more use of chemical herbicides. The overuse of these herbicides has resulted in an increase in herbicide-resistant weeds, making weed control difficult for farmers. This research aimed to …

Exploring The Biological Basis For Exercise Neuroprotection In Parkinson’S Disease: A Cross-Sectional Study Of Exercise And Physical Activity On Inflammatory Cytokines And Neurotrophins, Leah Isabelle P. Alegre, Jacob M. Brewer, Adeline Florschutz, Kate E. Hamiltonthompson

UNLV Theses, Dissertations, Professional Papers, and Capstones

Regular exercise and physical therapy are known to increase production of neurotrophins, including brain-derived neurotrophic factor (BDNF), and anti-inflammatory cytokines. These changes combine to theoretically protect and slow the progressive loss of neurons in Parkinson’s disease (PD). The purpose of this study was to examine the effect of exercise and physical activity on various anti-inflammatory and pro-inflammatory cytokines and neurotrophins in people with PD (PwP). This was a cross-sectional design involving PwP (n=37), aged 43-85 years, who participated in two 30-minute bouts of exercise (with brief rest breaks during and pre/post blood draws) separated by a week. Measures collected prior …

Postural And Load Dependent Modulation Of Lower Limb H-Reflex Excitability After Stroke, Daniel Bamiro, Matthew Berdiago, Ethan Hunter, Tia Nguyen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Purpose/Hypothesis

Leg stiffness is regulated by reflexive, intrinsic, and passive mechanisms, which are influenced by posture, body weight loading, and motor task demands. After a stroke, the capacity to modulate these mechanisms is often compromised. The H-reflex is a widely used electrophysiological tool to assess the excitability of the Ia afferent pathway within spinal circuitry. Although prior studies in healthy individuals have examined limb loading effects, results have been variable, and the role of postural and loading changes during standing remains underexplored, particularly in individuals post-stroke. This study investigated how different postural and limb loading conditions, specifically prone, standing, and …

Effectiveness Of Cognitive Stimulation Therapy On The Quality Of Life Of Adults With Dementia: A Secondary Analysis, Evelyn Airam

UNLV Theses, Dissertations, Professional Papers, and Capstones

This secondary analysis study evaluated the effectiveness of Cognitive Stimulation Therapy (CST) on quality of life (QoL), depressive symptoms, and cognitive function in 108 community-dwelling older adults with cognitive impairments, including dementia. Outcomes involved Quality of Life (QoL) measured by the Quality of Life in Alzheimer’s Disease (QoL-AD), symptoms of depression by the Cornell Scale for Depression in Dementia (CSDD), and cognitive function using the Saint Louis University Mental Status (SLUMS). After the CST, significant improvements were observed in QoL (p = .004), depressive symptoms (p = .002), and cognitive function (p < .001), with small effect sizes. Additionally, a significant interaction between sex and dementia diagnosis status was found in predicting cognitive changes, suggesting that the relationship between dementia diagnosis and cognitive improvement differs by sex. No between-group differences were found for QoL or depression. Findings support CST as a multidimensional, non-pharmacological intervention that improves cognitive, psychological, and social outcomes. Although effects were small, consistent improvements are clinically meaningful given the progressive nature of dementia. This study extends existing literature by demonstrating CST’s effectiveness in a community-based setting and reinforces its alignment with occupational therapy’s emphasis on participation and holistic care. Results highlight the need for early intervention and expanded access to CST programs to enhance quality of life in this population.

Unknowing Sacrifices: Public Health And Radiation Illness In New Mexico And Nevada, 1940s-1960s, Beatriz Avila-Marquez

UNLV Theses, Dissertations, Professional Papers, and Capstones

The detonation of the first atomic bomb in the early morning of July 16, 1945, in New Mexico welcomed the atomic age that forever changed the world. After that day, thousands of lives were lost due to nuclear weapons, and hundreds of thousands more continued to suffer from the effects of atomic testing. The Atomic Energy Commission, fueled by the arms race of the Cold War, chose Nevada to continue the United States’ nuclear weapons testing. Knowledge of the dangers of radiation exposure, the effects of radiation, and techniques to prevent exposure are now available in part due to the …

Junctional Actin As A Mechanical Hub For Supracellular Actomyosin Force Transmission During Apical Constriction, Jasneet Brar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Apical constriction is a conserved morphogenetic mechanism that drives epithelial folding during development. During ventral furrow formation in Drosophila melanogaster, contractile actomyosin networks generate forces that reduce apical cell area resulting in tissue invagination. For these cell-scale forces to produce coordinated tissue-scale changes, contractile networks must be mechanically integrated across neighboring cells. Although adherens junctions are known to mediate this coupling, it is unclear how junctions are integrated into cortical actin cytoskeleton, which directly determines cell, and consequently tissue, shape. This thesis tests the hypothesis that supracellular actomyosin organization during apical constriction depends on distinct adherens junction based mechanical couplings: …

Interpretable Deep Learning Models For Trustworthy Prediction Of Enzyme Functions, Louis Dumontet

UNLV Theses, Dissertations, Professional Papers, and Capstones

Trustworthy prediction of enzyme function from protein sequences remains a central challenge in computational biology, particularly when annotated data are limited, imbalanced, or incomplete. This dissertation develops interpretable deep learning methods for enzyme discovery and enzyme function prediction from amino acid sequences. First, it introduces PEPIC, an interpretable convolutional neural network for substrate-level prediction of hydrolytic plastic-degrading enzymes. Using curated and expanded sequence datasets, PEPIC improved predictive performance over benchmark methods, identified sequence regions aligned with catalytic and substrate-binding residues, and supported the discovery and experimental validation of a previously uncharacterized PET-degrading enzyme. Second, this dissertation investigates the integration of …

A Tale Of Two Mammals: Tenrec Ecaudatus And Homo Sapiens, Debra Renee Kerr

UNLV Theses, Dissertations, Professional Papers, and Capstones

Most modern mammals maintain stable body temperatures (Tb) ~37ºC and high metabolic rates. Hibernators, like ground squirrels, are homeothermic during the active summer season, but are heterothermic during the hibernation season. During the hibernation season, ground squirrels follow a predictable pattern of torpor bouts interrupted by interbout arousals. Tenrecs are unusual because they are heterothermic and heterometabolic even during the active season. Previously, ground squirrel bile constituents were shown to be predictive of whether a squirrel is in the summer active or winter hibernation season. We found tenrec bile constituents were highly variable and not predictive of seasonal and metabolic …

Methylation-Dependent Regulatory Pathway That Governs The Stability Of The Sox Family Proteins And Related Developmental Regulators, Keshari Gayathri Rajawasam

UNLV Theses, Dissertations, Professional Papers, and Capstones

The SRY (Sex-determining Region Y) protein is a transcription factor encoded on the Y chromosome and is the key regulator responsible for initiating male sex determination in mammals. During early embryonic development, SRY activates the genetic program that leads to testis formation by promoting the expression of downstream genes involved in male gonadal differentiation. Mutations or dysregulation of SRY can lead to disorders of sex development such as male-to-female sex conversion and hermaphroditism, highlighting its critical role in sex determination.

SRY belongs to the SOX (SRY-related HMG-box) family of transcription factors, which includes the proteins SOX1, SOX2 and SOX3. These …

Examining Demographic And Contextual Differences In Stress-Related Sport Interference Among U.S. Collegiate Sport Participants, Heather Tran

UNLV Theses, Dissertations, Professional Papers, and Capstones

Collegiate athletes experience a range of stressors that may interfere with sport performance; however less is known about how stress-related sport interference varies across demographic groups and contexts. Guided by the transactional model of stress and coping, this study examined differences in stress-related sport interference by gender, race/ethnicity, and year in school, as well as across situational contexts (training, competition, and life outside of sport). Data were drawn from a secondary dataset of 288 collegiate sport participants who completed the Sport Interference Checklist (SIC). Independent samples t-test and analyses of variance (ANOVA) were used to examined demographic differences, and a …

Application Of Occupational Therapy Task-Oriented For Chronic Post-Stroke Rehabilitation: A Case Study, Gia Nghi Vu

UNLV Theses, Dissertations, Professional Papers, and Capstones

Stroke remains the leading cause of long-term disability, with persistent upper extremity (UE) impairment limiting engagement in daily tasks, reducing quality of life and contributing to learned non-use in the chronic stage among stroke survivors. The Occupational Therapy Task Oriented (OTTO) approach is proposed to optimize occupational performance through the use of meaningful, functional and highly individualized tasks that enable clients to fulfill their valued roles and participate in desired daily activities. The purpose of this capstone project was to advance clinical skills by applying the OTTO approach to examine its effect on occupational performance and UE motor function in …

From Flight To Hatching: Adult Sexual Dimorphism, Life History, And Egg-Stage Ecology Of Pseudocotalpa Giulianii, Mandylene Mountain

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Nevada endemic dune beetle Pseudocotalpa giulianii is currently known only from two dune systems in Amargosa Valley, Nevada, yet much of its life cycle occurs below the sand surface and remains poorly documented. This thesis addresses key gaps in adult-stage biology and egg-stage development that are relevant to understanding reproduction, vulnerability, and habitat requirements in this dunerestricted species.

To characterize adult sexual dimorphism and life history, I quantified morphological differences between males and females and measured sex-specific adult survival under laboratory conditions relative to the aboveground flight season. Males and females overlapped broadly in external body size, but tarsal …

Skin Of Frog And Scale Of Dragon: Nutritional Cues, Life Cycle Kinetics, And Secretome Dynamics In Batrachochytrium Dendrobatidis, Naomi C. Okada

UNLV Theses, Dissertations, Professional Papers, and Capstones

Nearly 50% of amphibian species are at risk of extinction, making them the most threatened group of vertebrates on the planet. A major driver of these declines is the pathogenic chytrid fungus Batrachochytrium dendrobatidis (Bd), which causes the fatal skin disease chytridiomycosis. Since its discovery in the late 1990s, Bd has been directly implicated in the extinction of 90 species of amphibians and the declines of hundreds more. Despite this devastating ecological impact, relatively little is known about its basic cellular biology.

Bd has a multi-stage asexual life cycle consisting of a motile and infectious zoospore, which encysts (into a …

Protecting Galápagos’ Marine Ecosystems: Biosecurity And Network Design Against Invasive Species From Tourist Vessels, Marnie L. Campbell, T.U. Le, Chad Hewitt

Research outputs 2022 to 2026

The modern world is characterized by high connectivity, enhancing economic productivity, and global communication, but outpacing our ability to manage its consequences. This connectivity increases the risk of pathogen, pest, and invasive species transmission. In the Galápagos islands, Ecuador, tourist vessel networks operate with limited restrictions and connect high conservation value locations, boosting tourism and the economy. These vessels frequently travel between multiple islands, leading to moderate connectivity and high vessel promiscuity. To prevent the spread of invasive species via these vessels, we propose a biosecurity system that combines social network tools with risk management frameworks that balances environmental protection …

The Effect Of Stem Recutting And Floral Food On Vase Life Of Zinnias Elegans, Lauren Elise Baskin

Theses and Dissertations

A recent shift in the cut flower market focuses on locally produced cut flowers, creating interest in the consumer mind. The vase life of these cut flowers varies by variety and species. Knowing the proper post-harvest protocol is crucial information, as this affects flower’s longevity. This research used a 2x2x2 factorial design examining the zinnias variation in vase life between the following test groups: zinnias with trimmed and untrimmed stems in tap water and trimmed and untrimmed stems in floral food solution. The results of the study indicated that zinnias placed in a floral food solution and were trimmed have …

Community Development Professionals’ Perceptions Of Agritourism In Mississippi, Emma Jane Berry

Theses and Dissertations

Agritourism has emerged as a strategy for supporting rural economies, strengthening community identity, and increasing agricultural literacy. Although prior research has examined agritourism operators and visitors, limited attention has been given to the perceptions of community development professionals who influence local planning and program support. The purpose of this qualitative exploratory study was to examine community development professionals’ perceptions of agritourism’s role in community development, economic growth, and agricultural literacy in Mississippi. Guided by Social Exchange Theory, data were collected through two semi-structured virtual focus groups with 12 professionals representing Extension, tourism, economic development, and Main Street organizations. Data were …

Cytokinins, Gibberellins, And Ethylene On Plant Form And Flowering In Streptocarpus, Rachel Bobo

Theses and Dissertations

Plant form and flowering of Streptocarpus x hybridus Ladyslippers® ‘Grape Ice’ and ‘Deep Blue Vein’ were examined in response to exogenous application of ethephon [(2-chloroethyl) phosphonic acid], gibberellin 3 (GA3), benzyladenine (BA), and growth retardants ancymidol and chlormequat chloride. 750 mg L-1 ethephon applied at 3 weeks post-transplant (WPT) is effective in chemically pinching premature floral buds, delaying flowering by up to 10 days without restricting final plant size. Early applications of GA3 and BA hasten flowering by up to 10 days. GA3 and BA also interacted to promote floral development when applied early in the production cycle; increasing flower …

Determining Agricultural Literacy Proficiency Of Extension Professionals And Primary Volunteers In Mississippi, Molly Campbell

Theses and Dissertations

The development of agricultural literacy is a lifelong process, moving from gaining agricultural knowledge to communicating with others to applying to societal issues, and extends beyond the formal classroom. The Mississippi State University Extension Service has the ability to reach various audiences through educational programming, making them a potential catalyst for change in increasing agricultural literacy across the state. The study aims to determine the agricultural literacy levels of Extension professionals and primary volunteers in Mississippi and the factors potentially influencing these levels. Twenty-nine Extension professionals and eleven primary volunteers completed a survey of demographic questions and the Judd-Murray Agricultural …

From Miocene Origins To Ecological Diversification: Unraveling Evolutionary Dynamics Of North American Asters (Symphyotrichum), Sushil Dahal

Theses and Dissertations

Symphyotrichum, a plant genus traditionally treated within the genus Aster L., comprises >100 species of annual or perennial plants in the Compositae family Asteraceae. One of the most diverse genera in the southeastern US flora, the genus extends across the Americas, the Caribbean as well as eastern Eurasia. Despite its presence across the major American biomes and with distinctive floristic characteristics, several question regarding Symphyotrichum remains obscure. This research project focuses on exploring diverse aspects of Symphyotrichum systematics, biogeography and evolution. I tested three main hypotheses: (1a) Symphyotrichum originated ancestrally across Mexico and eastern North America in the Miocene ( …

Evaluating The Spray Patterns Of Boomless Spray Nozzles At Increasing Application Speeds, Thomas Hayden Duncan

Theses and Dissertations

Boomless nozzles have been largely ignored in spray studies, resulting in limited understanding of their spray patterns and how they are affected by operating parameters. A study was conducted to evaluate the effect of application speed on the performance of four boomless nozzles. Dyed water was applied to Kromekote® paper to assess spray distribution based on coverage data at three speeds, and MSMA was applied to a mixed turf stand to evaluate the effect of speed on performance. Overlap simulations were done to evaluate how uniformity could be improved, and nozzle performance was compared to manufacturer claims. Spray patterns of …

Assessment Of Energy And Hydration Demands Of Farm Workers In Mississippi, Mckenzie Lynne Hargrove

Theses and Dissertations

The agricultural community in Mississippi is critical to the state’s economy and the health of its communities. Farm workers meet these needs by performing highly demanding work with long hours of labor, limited resources, and stressful environmental conditions. The purpose of this investigation was to examine and measure indicators of physiological and behavioral adaptations to the heat such as whole body sweat rate (WBSR), sweat sodium (Na+) content, urine specific gravity (USG), and fluid intake, as well as determine total daily energy expenditure (TEE) and water influx (rH2O) via the doubly labeled water technique (DLW) in farm workers during the …

Assessing Biotic And Abiotic Drivers Of Hybridization And Species Delimitation In A Highly Reticulate Syngameon, Nicholas John Engle-Wrye

Theses and Dissertations

Hybridization, or the flow of genetic materials between varieties or species, shapes evolution by generating novel genotypes, altering allele frequencies, and driving diversification. Yet, the rate at which hybridization occurs is heterogeneous across the tree of life and the ecological and evolutionary factors that govern its occurrence remain poorly understood. This dissertation examines how conserved biotic and abiotic niches influence hybridization and uses integrative methods to delimit species boundaries within Heuchera (Saxifragaceae), a genus notable for widespread interspecific gene flow and cryptic morphological diversity. Results reveal that hybridization is most frequent where pre- and postzygotic barriers are weak, as with …

Evaluation Of Critical Nitrogen Requirements For Commercial Tomato Production In Mississippi, Prakash Khanal

Theses and Dissertations

Optimizing nitrogen (N) management is critical for tomato production, as crop requirements vary with soil, climate, and production practices. Despite the importance of tomato in Mississippi, region-specific N recommendations remain limited. Field studies (2024-2025) and a high tunnel study (2025) were conducted at the North Mississippi Research and Extension Center to determine critical N rates for fresh-market tomato. Six N rates were evaluated using a randomized complete block design with four replications. Results provide system-specific guidance, highlighting differences in N requirements between open-field and high tunnel production. Tailoring N inputs to production environments can improve yield and fruit quality, enhance …

Reproductive Genetics Of Caenorhabditis Nematodes, Dharani U. Matharage

Theses and Dissertations

Variation in reproductive traits and genome evolution reflects the interaction between selection, environment, and life-history. In the genus Caenorhabditis, recombination rate and sperm size exhibit a striking variation between strains and species. Here, Iintegrate three projects investigating recombination rate variation and recombination plasticity in C. elegans and sperm size divergence across Caenorhabditis species. First, I quantify recombination rates in two genetically distinct strains, N2 and CB4856, with different levels of laboratory adaptation using fluorescent markers spanning across gene-rich and gene-poor regions of the chromosome IV. I identify significant differences in recombination rate between the two strains within both gene-rich and …

Characterizing The Microbial Structure And Function In Black Soldier Fly Digested Dairy Manure And Subsequent Larvae, Branden N. Pierce

Theses and Dissertations

This work examined the microbial structure and gene expression found in Black Soldier Fly larval guts and their frass when reared on dairy cattle manure that had been amended with either R. rhodococcus 21198, P. polymyxa 8158, or both and compare the results to larvae reared on untreated manure. First an overview of literature surrounding projected global food supplies and the possible use of insects to either increase them or mitigate the environmental effect of producing said supply. A cellulose degradation assay was performed to assist in determining which bacterial species to use as amendments. Characterization of the microbial communities …