Digital Commons Network^™

Ifnγ-Driven Skewing Towards Th1 Over Th17 Differentiation Underlies Crs And Neutropenia In Car-T Therapy, Payal Goala

USF Tampa Graduate Theses and Dissertations

CAR-T therapy has improved overall and complete response rates, andprolonged progression free survival of patients with relapsed/refractory hematological malignancies. The short-term and long-term impacts of anti-CD19 CAR-T products on the immune landscape of B cell malignancies has garnered considerable attention in recent years. Most notably, a subset of patients experience high-grade cytokine toxicities, including cytokine release syndrome and immune cell-associated hematologic toxicity that manifest as prolonged cytopenia. Whereas etiology of CRS is more well established, the mechanisms leading to CAR-T associated cytopenia remains elusive. Rejeski et. al. showed that severe cytopenia in patients post CAR-T treatment led to frequent infections …

Interview With Orca Award Winner John J. Janovy, Jr., Unl Biological Sciences; Parasitologist And Author, Mark Griep, John J. Janovy Jr.

UNL ORCA Interview Series

Professor John Janovy, Jr., was awarded the ORCA in 1998. John was born in Louisiana, but his family moved to Oklahoma, where he grew up in Tulsa and then Oklahoma City. Something in his youth must have sparked an interest in science because he earned three degrees from the University of Oklahoma in Norman: a bachelor of science in math in 1959, a master of science in zoology in 1962, and doctoral degree in zoology in 1965. Next, he ventured 1400 miles east to Rutgers University in New Brunswick, New Jersey, where he did post-doctoral work for about a year. …

Electrochemical Sensors For In Situ Monitoring Of Reactive Species During Cold Atmospheric Plasma-Based Therapies, Jonathan Thomas, Suneel Kumar, Gagana Karkada, Julia Sutter, Kristina Pattison, Jason Rainone, Dhruv Patel, Shashank Madhavan, Fred Krebs, Francois Berthiaume, Vandana Miller, Katharina Stapelmann

Kimmel Cancer Center Faculty Papers

Cold atmospheric pressure plasma (CAP) is emerging as a clinically relevant therapy for dermatological conditions such as actinic keratosis, warts, and chronic wounds. However, these therapies lack strategies to monitor CAP delivery in situ and to ensure delivery of an effective CAP dose without unwanted toxicity. CAP acts as a therapeutic agent in these biomedical applications primarily (but not solely) through reactive oxygen and nitrogen species (RONS) generated at transiently high local concentrations. Here we demonstrate the use of bio-electrochemical sensors capable of real-time measurements of key CAP RONS: hydrogen peroxide and oxidation-reduction-potential (ORP). In in vitro scratch assays and …

Comparative Analysis Of Color, Texture, And Water Content In Three Shallot Cultivars At Different Harvest Ages, Rahma Wati Lestari, Kusumiyati, Syariful Mubarok

Jurnal Kultivasi

Shallots are a major horticultural commodity with high economic value. The quality of shallots is determined not only by the quantity of the harvest but also by physical characteristics such as bulb color, texture, and water content. This study aims to evaluate the differences in these physical parameters in three popular local cultivars: Brebes, Sumenep, and Tuktuk, harvested at 56, 63, and 70 days after planting (DAP), respectively. This research uses a Randomized Block Design consisting of 9 treatment combinations and analyzed using the SmartstatXL application. Color measurements are conducted using the CIELAB method and converted to CIELCH and RGB …

Role Of Nano Edible Coating, Packaging, And Storage Temperature In Maintaining Color, Moisture Content, And Phenolic Compounds Of Straw Mushroom, Kiki Nuratni Sitompul, Syariful Mubarok, Kusumiyati

Jurnal Kultivasi

Straw mushrooms are among the most sought-after edible mushrooms, but their harvest is highly perishable due to their high respiration and transpiration rates, leading to a decline in quality during storage. The application of nano edible coating can cover the surface of straw mushrooms and maintain their freshness, while packaging and storage at low temperatures can maintain quality by inhibiting respiration and enzymatic activity. This study aims to evaluate the effects of nano edible coating, packaging types, and different storage temperatures on the color, moisture content, and phenolic compounds of straw mushrooms. The study was conducted at the Horticulture Laboratory, …

A Review Of Bioactive Compounds Of Caesalpinia Sappan: Pre- And Post-Harvest Effect, Annisa Lugina Rachman, Santi Rosniawaty, Syariful Mubarok, Cucu Suherman, Yudithia Maxiselly

Jurnal Kultivasi

Sappanwood (Caesalpinia sappan L.) is a shrub or small tree that thrives in tropical regions and has been widely distributed across various areas. It has long been used as a natural dye in textiles, cosmetics, and herbal beverages due to its content of various bioactive compounds. This literature review discusses the secondary metabolites in sappanwood, their health benefits, and strategies to enhance product quality through pre-harvest and post-harvest treatments. This literature review was conducted by searching for relevant journals on Google Scholar using relevant keywords. Sappanwood contains diverse secondary metabolites, including flavonoids, phenolics, anthraquinones, triterpenoids, steroids, alkaloids, and tannins, with …

Spike Timing Depended Plasticity Produces Unsupervised Learning Of Synergistic Muscle Feedback In A Synthetical Neural Network, Mark Allen Pupkiewicz

Dissertations and Theses

This study investigates how type Ia feedback from muscle spindles can be organized into groups representing agonistic muscle pairs through Spike Timing Dependent Plasticity (STDP). A single degree of freedom joint is actuated with four biologically modeled muscles forming two agonistic pairs. In order to emulate the sensory dynamics of biological muscle spindles, sensors in the model record the active length and velocity states of each muscle, the two primary factors eliciting type Ia afferent responses. In biological networks, synapses from Ia sensory neurons frequently activate interneurons representing agonistic muscle sources. This research investigates whether this organization can emerge in …

Mechanistic Modelling And Parameter Estimation Of Heterogeneous Catalytic Synthesis Of Green Fuel, Rasheed U. Owolabi, Obinna P. Anele, Mohammed A. Usman

Mansoura Engineering Journal

Investigation of the kinetics and mechanistic modelling of biodiesel synthesis through the reaction of castor oil with methanol under catalyzed conditions was carried out in this study. The reaction mechanisms of the Eley-Rideal, Langmuir-Hinshelwood-Hougen-Watson, and Hattori type were employed to identify the rate-determining or controlling step of the reaction. Thirteen rate expressions were developed for reactant adsorption, product desorption, and surface chemical reactions. The experimental data from Sanchez-Cantu et al. were used to validate the models, and the best-fitting model and parameter estimation were evaluated using SAS and MATLAB software. From the results, the Eley-Rideal mechanism with glycerol desorption as …

Mitochondrial Insoluble Aggregates In Aging And Alzheimer’S Disease Models: Cross-Species Proteomic Integration, Identification Of Conserved Candidates, And Pharmacological Disruption Of Eef-2 Interactions, Sonu Pahal

Theses and Dissertations

Protein aggregation within mitochondria emerges early in both aging and Alzheimer’s disease (AD) and contributes to progressive loss of proteostasis. Insoluble mitochondrial aggregate fractions from Caenorhabditis elegans revealed extensive enrichment of metabolic and translational proteins that also appear in human Alzheimer hippocampal aggregates. Comparative analysis across age and genotype identified a conserved subset of aggregation-prone proteins, several of which cluster around elongation factor 2 (eEF-2), a regulator of ribosomal translocation and energy coupling. Cross-species integration defined a network in which eEF-2 links chaperones, metabolic enzymes, and RNA-binding proteins that co-aggregate in both human Alzheimer material and nematode models. Functional tests …

Space Agriculture: A Comprehensive Systems-Level Review Of Challenges And Opportunities, Hassan Fazayeli, Aaron Lee M. Daigh, Cassandra Palmer, Santosh Pitla, David Jones, Yufeng Ge

Biological Engineering Faculty Publications

As humanity prepares for prolonged space missions and future extraterrestrial settlements, developing reliable and resilient food-production systems is becoming a critical priority. Space agriculture, the cultivation of plants beyond Earth (particularly on the Moon and Mars), faces a constellation of interdependent environmental, biological, and engineering challenges. These include limited solar radiation, elevated ionizing radiation, large thermal variability, non-Earth atmospheric pressures, reduced gravity, regolith substrates with low nutrient-holding capacity, high-CO₂/low-O₂ atmospheres, pervasive dust, constrained water and nutrient availability, altered plant physiology, and the overarching need for closed-loop, resource-efficient systems. These stressors create an exceptionally challenging environment for plant …

Improving Profitability Of Small And Medium Sized Farms Though Economic Optimization Of Wheel-Line Irrigation, John B. Barker, Ryan A. Larsen, Matt A. Yost

Funded Research Records

No abstract provided.

Investigation Of Coral Reefs For Coastal Protection: Hydrodynamic Insights And Sustainable Flow Energy Reduction, Faisal Karim, Napayalage A. K. Nandasena, James P. Terry, Mohamed M. Mohamed, Zhonghou Xu

All Works

Coral reefs are integral components of tropical coastal marine ecosystems that have considerable capacity to mitigate extreme flows and marine floods caused by storms and tsunamis. However, limited studies on coral reef efficacy in reducing such flows, coupled with variable roughness coefficient characteristics, hinder their broader utilization in sustainable engineering applications for societal benefit. In this study, we conducted comprehensive experimental investigations to examine flow–coral interactions and the flow energy reduction capabilities of coral reefs. Three-dimensional-printed coral reefs were used to simulate actual coral reefs, providing a scalable and environmentally responsible approach for studying nature-based coastal protection systems. Flow characteristics …

Engineered Multifunctional Drug-Loaded Dendrimer Nanoparticles For Glaucoma: Triple Mechanism Via Antioxidant Activity, Iron Chelation, And Enhanced Ocular Transport, Burhan Ates, Ashish Trital, Vimalin Jeyalatha Mani, Lei Xu, Jonathan Kenlee, Nuran Ercal, Hu Yang

Chemistry Faculty Research & Creative Works

In this study, we developed multifunctional nanoparticles based on polyamidoamine (PAMAM) dendrimers functionalized with caffeic acid (CA) and poly (ethylene glycol) maleimide (PEGM) for the topical delivery of hydrophobic antiglaucoma drugs brimonidine (BM) and betaxolol (BX). The PAMAM-CA and PAMAM-CA-PEGM conjugates exhibited antioxidant and iron-chelating activities in a dose-dependent manner. BM- and BX-loaded dendrimer nanoparticles produced using a multi-inlet vortex mixer showed uniform spherical morphology (∼80 nm by TEM) and hydrated sizes of ∼135-144 nm by DLS. Both nano formulations demonstrated high cytocompatibility with human corneal epithelial cells and were nonirritant in the HET-CAM assay, with PEGM further improving cytocompatibility. …

Measuring Levels Of Ultrashort-Chain Per- And Polyfluoroalkyl Substances In Blood Collection Tubes, Alina M. Dziembowski

Discovery Undergraduate Interdisciplinary Research Internship

Per- and polyfluoroalkyl substances (PFAS) are highly persistent synthetic chemicals that are now ubiquitous in the environment. Due to PFAS stability leading to its environmental persistence, there is concern regarding potential long-term health effects as PFAS accumulation occurs in humans from dietary and inhalation exposure routes. Chronic exposure may lead to adverse effects such as fertility issues or liver damage. Although a wide range of PFAS have been found in human samples, specifically human serum, there remains data gaps for what PFAS are present in human blood. Recent innovation in detection technology has led to the ability to more accurately …

From Collision To Coexistence: Data-Driven Approaches To Reducing Wildlife Rehabilitation Center Intakes, Alice Anastos

Honors Projects

This project investigates the primary anthropogenic drivers of wildlife admissions at Lake Erie Nature and Science Center using a quantitative analysis of 10,172 intake records from the WILD-ONe database. Window collisions were by far the leading cause of admission, accounting for 54% of all intakes. Orphaning represented 12%, followed by vehicle collisions (5%) and domestic cat attacks (4%). By integrating findings from current wildlife rehabilitation research, this study identifies evidence-based strategies, such as bird-safe window treatments, public education on natural animal behavior, and targeted urban planning, that can substantially reduce preventable injuries. These results underscore the need for context-specific outreach …

Evaluating Remotely Sensed Spectral Indices To Quantify Seagrass In Support Of Ecosystem-Based Fisheries Management In A Marine Protected Area Of Western Australia, Nick Konzewitsch, Lara Mist, Scott N. Evans

Fisheries Research Articles

Highlights

• Four spectral indices were identified as important for the quantification of seagrass within and adjacent to the MSC-certified Western Australia Enhanced Greenlip Abalone Fishery. The Normalised Difference Aquatic Vegetation Index (NDAVI) and Depth Invariant Index of the blue and green bands were the most important indices.
• Similar seagrass cover and distribution were observed inside and outside of the fishery area of operation.

• The use of indices from free satellite products via Google Earth Engine workflows and automatic image annotation provides a rapidly repeatable method to support …

Environmental Risk Assessment Of Textile Waste From Bio-Engineered Fibers In The Fashion Industry, Aayush Pankaj Agarwal, Harshit Soral, Irmak Aktan, Karol Montserrat Gonzalez Saad, Maria Daniela Saa Espinosa, Sheetal Suhas Dhanuka, Yi Yang

Harrisburg University Dissertations and Theses

The global fashion industry generates over 92 million tons of textile waste annually, a figure projected to increase significantly by 2030. Bio-engineered fibers, including bacterial cellulose, mycelium-based leather, and recombinant protein textiles emerge as sustainable alternatives to conventional fibers. Despite their promise, recent evidence suggests that these fibers may pose environmental risks at the end-of-life stage, such as incomplete biodegradation in landfills, microfiber shedding, and chemical leaching from dyes and coatings. The purpose of this study is to examine how these risks are represented through scientific literature, corporate sustainability reports, and policy frameworks, and to identify strategies for integrating risk …

Fgf13 Regulates Vgsc-Independent Cardiomyocyte Impulse Propagation Via Cx43 Trafficking, Lala Tanmoy Das, Mattia Malvezzi, Aravind R Gade, Maiko Matsui, Margaret Mckay, Matea J Zelich, Jared Kushner, Bi-Xing Chen, Isabella Distefano, Daniel Roybal, Lin Yang, Lisa Stoll, James C Lo, Marian Kalocsay, Fadi G Akar, Steven O Marx, Geoffrey S Pitt

Faculty, Staff and Student Publications

Background: FHF (fibroblast growth factor homologous factor) variants associate with arrhythmias. Although FHFs are best characterized as regulators of voltage-gated sodium channel (VGSC) gating, recent studies suggest broader, non-VGSC-related functions, including regulation of Cx43 (connexin 43) gap junctions and hemichannels, mechanisms that have generally been understudied or disregarded.

Methods: We assessed cardiac conduction and cardiomyocyte action potentials in mice with constitutive cardiac-specific Fgf13 ablation (cFgf13KO) while targeting Cx43 gap junctions and hemichannels pharmacologically. We characterized FGF13 regulation of Cx43 abundance and subcellular distribution. With proximity labeling proteomics, we investigated novel candidate mechanisms underlying FGF13 regulation of Cx43. …

Facility Usage On The International Space Station, Justin St. P. Walsh, Rao Hamza Ali, Ryan Shihabi, Erik Linstead

Art Faculty Articles and Research

Science experiments on the International Space Station (ISS) and in future space habitats require “facilities,” as they are known to space agencies, as the core building blocks of the research capabilities they provide. Facilities are experimental apparatus that enable research, often (but not always) used for more than one experiment, such as centrifuges, furnaces, gloveboxes, freezers, and more. The US Orbital Segment of the ISS has been home to 191 such facilities, belonging to four of the five ISS international partner agencies as well as private companies, in its 25 years of continuous operation. Until now, there has been no …

Automated Detection Of Kinky Back In Broiler Chickens Using Optimized Deep Learning Techniques, Ramesh Bahadur Bist, Andi Asnayanti, Anh Dang Trieu Do, Yang Tian, Chaitanya Palleria, Dongyi Wang, Adnan Alrubaye

Food Science Faculty Publications and Presentations

The global poultry industry faces growing challenges from skeletal disorders, with Kinky Back (KB) significantly impacting broiler welfare and production. KB causes spinal deformities that reduce mobility, feed access, and increase mortality. It often remains undetected in early subclinical stages. Traditional KB diagnosis methods are slow and subjective, and highlighting the need for an automated and objective detection. This study develops a machine learning approach for detecting KB in broilers using image data. Male Cobb 500 broilers were raised under controlled conditions and monitored over 7 weeks using overhead 4K video cameras. Behavioral and posture data related to KB were …

Exploring The Relationship Between Loneliness And Real-Time Measurements Of Gsr And Hrv During Concept Mapping, And Cortisol And Oxytocin Levels, Jangwon Seo

Masters Theses

This study attempts to explore loneliness among college students from a bio-physio-psycho-social perspective, and it utilizes real-time physiological measurements (GSR and HRV) and biomarkers (cortisol and oxytocin) in conjunction with psychosocial assessments. The study employs both qualitative and quantitative methods, which involve concept mapping exercises, with the aim of comprehending the full nature of how loneliness interacts with physiological and psychological processes. At Time 1 (T1) we found significant positive correlations between loneliness and elevated GSR levels, which is indicative of increased sympathetic arousal (r = .35, p < .01). Additionally, there were significant negative correlations between loneliness and mean RR intervals in HRV that indicated autonomic dysregulation featured by increased sympathetic activities as well as reduced parasympathetic functions (r = -.28, p < .05). However, at Time 2 (T2), these correlations were not significant indicating possible temporal fluctuations due to adaptive responses or extraneous variables such social support systems or coping mechanisms. The qualitative data from concept mapping enriched the analysis by providing deeper insights into the cognitive and emotional processes underlying loneliness. Despite limitations like demographic skewness, funding constraints, logistical challenges, etc., this, therefore, highlights the need for multidimensional investigations of psychological phenomena. This has implications for future research as well as clinical practice, which could include using these integrative measures to develop holistic interventions for mental health disorders.

The Clinical Pharmacogenetics Implementation Consortium's Consensus-Based Framework For Assigning Allele Function., Bailey M. Tibben, Andrea Gaedigk, Li Gong, Katrin Sangkuhl, Michelle Whirl-Carrillo, Mary V. Relling, Roseann S. Donnelly, Teri E. Klein, Kelly E. Caudle

Manuscripts, Articles, Book Chapters and Other Papers

The Clinical Pharmacogenetics Implementation Consortium (CPIC) is dedicated to integrating pharmacogenetic testing into clinical practice by developing and disseminating peer-reviewed, evidence-based gene-drug clinical practice guidelines. A critical component of this effort is the assignment of clinical function to pharmacogene alleles, which informs the translation of genetic test results into actionable prescribing decisions. This technology review outlines the standardized procedures and framework used by CPIC to assign allele clinical functional status through the work of Pharmacogene Curation Expert Panels (PCEPs). These panels, comprising multidisciplinary experts, systematically review and evaluate evidence to assign functional status to pharmacogenetic haplotypes. The process includes rigorous …

Hsp90 Buffers Deleterious Genetic Variations In Brca1, Brant Gracia, Xing-Han Zhang, Patricia Montes, Tin Chanh Pham, Min Huang, Georgios Ioannis Karras

Faculty, Staff and Student Publications

Protein-folding chaperone heat shock protein 90 (HSP90) buffers genetic variation in diverse organisms, but the clinical significance of HSP90 buffering in human disease remains unclear. Here, we show that HSP90 buffers mutations in the BRCT domain of BRCA1. HSP90-buffered BRCA1 mutations result in protein variants that retain interactions with partner proteins and strongly rely on HSP90 for protein stability and function in cell survival. Moreover, HSP90-buffered BRCA1 variants confer poly (ADP-ribose) polymerase (PARP) inhibitor resistance in cancer cells. Low-level HSP90 inhibition overcomes this resistance, revealing a cryptic and mutant-specific HSP90-contingent synthetic lethality. Furthermore, by stabilizing metastable variants across the entirety …

Using Artificial Intelligence Applications In Humanizing Temporary Buildings For Workers, Developing Design Standards And Involving Users, Anas Almahmoud, Hatem El Shafie

Mansoura Engineering Journal

This research focuses on artificial intelligence (AI) and its tools for assisting in architectural design and decision-making. It identifies modern AI applications for use in research and design processes, aiming to raise awareness among architects and consulting firms. This is the primary objective of the research. The study also analyzes a mobile temporary worker cabin complex in Riyadh, specifically the metro project, as a case study. The goal is to address its architectural program, improve its design, identifies the necessary design criteria and make it more humane. The core research problem lies in testing the effectiveness of using AI tools …

Molecularly Imprinted Polymer For The Removal Of Nutrients From Water: Synthesis, Application, Performance And Limitation, Emmanuella Anang, Abolade Busari

Faculty Publications

Nutrient contamination is a major contributor to eutrophication and water quality degradation worldwide. Conventional treatment technologies often lack selectivity and efficiency in complex aquatic environments, highlighting the need for advanced materials with tailored recognition capabilities. Molecularly imprinted polymers (MIPs) have emerged as promising adsorbents for nutrient remediation due to their high selectivity, stability and reusability. This review synthesizes recent progress on the synthesis strategies of MIPs. Applications of MIPs in removing phosphate, nitrate and ammonia from water are critically examined, with particular attention to adsorption performance under varying environmental conditions. The limitations of current systems, including modest adsorption capacities, incomplete …

Unsaturated Fatty Acid Oil-Based Microdroplets: A Promising Novel Class Of Microdroplets, Ramiz S. Alejilat

Seton Hall University Dissertations and Theses (ETDs)

Droplet-based microfluidics has rapidly advanced numerous fields, including chemistry, biology, materials science, medicine, food science, and cosmetics. In these systems, fluorocarbon oil combined with fluorinated surfactants is the preferred medium for fluid encapsulation, offering exceptional stability and biocompatibility essential for sensitive biological and chemical processes. However, growing concerns about the environmental and biological risks associated with fluorinated chemicals have prompted a search for alternatives.

This study is the first to explore the use of unsaturated fatty acids derived from emu oil for microdroplet formation. We characterized droplet formation based on flow rates and the presence non-fluorinated surfactant at a certain …

Emerging Tools In Plant Genome Editing, Shilpi Sharma, Naveen Kumar Saroha, Abhilasha Sehrawat, Guiliang Tang, Deepali Singh, Sachin Teotia

Michigan Tech Publications

Plant genome editing has undergone a transformative shift with the advent of advanced molecular tools, offering unprecedented levels of precision, flexibility and efficiency in modifying genetic material. While classical site-directed nucleases such as ZFNs, TALENs and CRISPR-Cas9 have revolutionized genome engineering by enabling targeted mutagenesis and gene knockouts, the landscape is now rapidly evolving with the emergence of novel systems that go beyond the conventional double strand break (DSB)-mediated approaches. Advanced and recent tools include LEAPER, SATI, RESTORE, RESCUE, ARCUT, SPARDA, helicase-based approaches like HACE and Type IV-A CRISPR system, and transposon-based techniques like TATSI and piggyBac. These tools unlock …

The Impact Of Infrastructure Construction And Remodeling Of Cities On Its People' Quality Of Life Miraj District-(Madi-Cairo) As A Geological Heritage Area, Ahmed M.E. Hammad

Mansoura Engineering Journal

Rapid urbanization is increasing globally, the urbanization rate expected to rise to more than 66% by 2050. (According to UN estimates) the increasing rates of urbanization in the world means adding 2.5 billion people to the urban population within a short period. Which necessitates increasing the traffic axes and the rest of the networks in terms of lengths and widths within the existing cities, and with the presence of the population during these works - despite their inevitability - the quality of their urban life is affected socially, naturally and economically. the research monitors these effects and the extent of …

Edna Surveys For The Critically Endangered Caribbean Staghorn Coral, Acropora Cervicornis (Lamarck, 1816), Pari Elaine Cribbins

All HCAS Student Capstones, Theses, and Dissertations

Historically, Acropora cervicornis was a dominant coral on Caribbean reef systems, but an outbreak of white band disease in the late 1970s marked the beginning of devastating, multi-decadal population declines that led to the coral being listed as “critically endangered” on the IUCN Red List in 2022. Today, after a record-setting marine heat wave in 2023 triggered a mass bleaching event on Florida’s Coral Reef (FCR), A. cervicornis is considered functionally extinct on the FCR. While individuals of A. cervicornis do remain on the FCR, the small population size of the coral makes in situ surveys difficult. However, surveys using …

Longitudinal Pharmacokinetic And Safety Studies Of An Antibody-Erythropoietin Fusion Protein For Alzheimer's Disease, Rudy Chang, Devaraj Venkatapura Chandrashekar, G. Chuli Roules, Nataraj Jagadeesan, Emi Iwasaki, Adenike Oyegbesan, Hayk Davtyan, Rachita K. Sumbria

Pharmacy Faculty Articles and Research

Erythropoietin (EPO) shows promise for Alzheimer's disease (AD) but has poor brain penetration, necessitating high doses that cause hematopoietic side effects. To improve brain delivery, EPO was fused to a transferrin receptor monoclonal antibody (TfRMAb), and this study evaluated the pharmacokinetics (PK), safety, and efficacy of repeated TfRMAb-EPO dosing in mice to further its preclinical development. C57BL/6J male mice (10 weeks old, n = 4–5/dose) received subcutaneous (SQ) low (1 mg/kg), mid (3 and 6 mg/kg), or high (20 mg/kg) TfRMAb-EPO doses for 4 weeks. The 1 mg/kg dose showed no adverse effects and resulted in sustained brain and plasma …