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Enzymatic Synthesis Of Oligoribonucleotides Of Defined Base Sequence, Bronwyn Geraldine Hughes

Theses and Dissertations

A possible method for the synthesis of ordered oligoribonucleotides involves primer-dependent polynucleotide phosphorylase (PNPase) synthesis using the RNase A or T1 resistant N-cyclohexyl-N'-β(4-methylmorpholinium)ethylcarbodiimide chloride (CMC-Cl) derivatives of uridine or guanosine containing primers. Thus, CMC-UpA, CMC-GpA, CMC-UpCpC, CMC-GpApC, and ApApApApU-CMC were prepared and studied as primers for PNPase in 15 min 14C-ADP polymerization reactions and also in 6-10 hr synthesis reactions. The CMC-primers were not suitable primers for PNPase catalyzed synthesis reactions for either time. The PNPases used in such studies were contaminated with nuclease that showed the following order of susceptibility: UpA > CpC, GpA > ApU, ApA. Even a highly purified …

Reproduction And Ecology Of The Jemez Mountains Salamander, Plethodon Neomexicanus, Stephen R. Williams

Biology ETDs

Plethodon neomexicanus is a salamander endemic to the Jemez Mountains of New Mexico. Only about 10 localities are known, and in the study area one salamander occupies about 10 m².Adult females are significantly larger than adult males, and the sex ratio is about one for the entire active season which extends from early June to early October. Sexual maturity is reached after 2 to 3 years in males and after 3 years in females. The diurnal ecological maximum and minimum temperatures are 17.0 and 6.0 C, respectively, with an average habitat temperature of 12.2 C. Most of the …

The Boraginaceae Of Utah, Larry C. Higgins

Brigham Young University Science Bulletin, Biological Series

This paper presents a revision of the family Boraginaceae for the state of Utah. Ninety-three species in seventeen genera are recognized. The treatment includes keys, synonymy, descriptions, and citations of representative specimens found in the various herbaria in the state of Utah. The new combination Lappula occidentalis (Wats.) Greene var. cupulata (Gray) Higgins is presented.

Special Report On Zoos

Special Reports

In many ways, The Humane Society has become the Ralph Nader of the zoo world, spurring into action the individuals or municipalities who own zoos, the staffs who operate them, the taxpayers who are in the end responsible for their existence, and the federal officials who are now required to ensure that they meet certain standards.

South Dakota Farm And Home Research, Agricultural Experiment Station, South Dakota State University

South Dakota Farm and Home Research: 1949 -1998

Controlled Burning as a Range Management Tool [p] 3
Farmers DO Use Earmuffs [p] 6
Remote Sensing Helps Locate Problem Soils [p] 7
Range condition and runoff in Western South Dakota [p] 11
A Hidden Treasure Ups Net Income [p] 14
Passport to 1874 [p] 17

A New Species Of Menoceras From The Marsland Formation Of Nebraska, Lloyd G. Tanner

Bulletin of the University of Nebraska State Museum (1924–2023)

Further study indicates that the Menoceras remains collected from the "Bridgeport Quarries" (lower port:on or the Marsland Formation, Middle Miocene, of the Hemingford Group) are of a new species.

In 1962, these dicerathere remains were considered to be a geologic variety of Diceratherium niobrarensis (Stecher, Schultz, and Tanner). However, later (Tanner, 1969) a generic distinction was revived, separating the Menoceras Troxell from Diceratherium Marsh. Diceretherium niobrarensis was then placed in synonymy with Menoceras arikarense (Barbour).

The new species is an intermediate between Menoceras arikarense (Barbour) from the Harrison Formation and Menoceras marslandensis Tanner, from the upper portion of the Marsland …

Two Lynx-Like Cats From The Pliocene And Pleistocene, C. Bertrand Schultz, Larry D. Martin

Bulletin of the University of Nebraska State Museum (1924–2023)

A new species (Lynx stouti) of small felid is described from the lower Pliocene of Colorado. This form has several characters in common with the modern Lynx and may be ancestral to that genus. A new subspecies of Lynx issiodorensis Croizet and Jobert is described as L. i. kurteni from the Mullen Assemblage, Cherry County, Nebraska. The relationships of this form to other lynxes are discussed along with the paleo-distribution of the genus.

The classification of the felinae has always been somewhat controversial, especially at the generic level. One fairly homogenous group of cats which has been separated …

Annual Report And Membership List 1971-72, Iowa Academy Of Science

Iowa Academy of Science Documents

Contents:

Minutes of the Annual Business Meeting --- 4
Report of the Executive Secretary --- 8
Report of the 84th Session --- 14
Report of the Social Implications Committee --- 16
Report of the Finance Committee --- 18
Treasurer's Report --- 20
Report of the Librarian --- 22
Report of the Recognition and Awards Committee --- 23
Report of the Student Programs Committee --- 26
Report of the Articulation Committee --- 29
Report of the Resolutions Committee --- 31
Report of the Membership Committee --- 32
Constitution, Bylaws, and Articles of Incorporation --- 33
Constitution --- 34
Bylaws --- 38 …

The Role Of The Adrenal Gland In The Survival Of White Rats Subjected To The Parotid Toxin Of The Toad, Bufo Marinus, Mark A. Green

Master's Theses

Six groups of white labor atory rats were utilized in this experiment. A sham-operated group and an adrenalectomized group were used as controls. Four other groups were adrenalectomized and given replacement therapy of cortisone, deoxycorticosterone acetate, epinephrine, and norepinephrine. All groups were then given injections of toad toxin from the parotid glands of Bufo marinus. Their heart and breathing rates were recorded for 60 minutes.

All of the untreated adrenalectomized rats showed altered heart and breathing rates and were killed by the toxin. Cortisone and nor ­ epinephrine aided the adrenalectomiz ed rats to survive. but cortisone was the …

Spacing And Orientation Of The Siphons Of The Tunicate, Styela Plicata, Alfred Page Chestnut

Master's Theses

The spacing and orientation of sipons of Styela plicata (Lesueur)in swift and slow currents were examined. Within aggre­ crates, the siphons were spaced farther apart in slow currents than in swift currents. The siphons were not horizontally oriented but were vertically oriented in slow currents. The adaptive values of spacing and orientation under different currents are discussed.

Quantitative Growth Relationships Of Microcoleus Vaginatus (Gom.) Ex Drouet And Alcaligenes Sp. In A Sucrose-Salts Medium, James Rexford Brownell

Master's Theses

The blue-green alga, Microcoleus vaginatus (Gom.) ex Drouet 1968, and a bacterium, Alcaligenes sp., collected from the same habitat, were cultured together and separately in mineral salts media with and without sucrose. Growth of axenic M. vaginatus was stimulated by sucrose. In association with the bacterium in sucrose-containing media algal growth was suppressed, while in inorganic media it was stimulated. Factors involved in this algal-bacterial system probably included competition for the carbon source, changes in pH, and accumulation of cellular waste and lytic products.

An Electrophoretic Comparison Of The Gill Plasma Membrane Proteins Of Freshwater And Saltwater-Adapted Killifish, Fundulus Heteroclitus, Earl Dean Hammit

Master's Theses

A study was undertaken to compare proteins present in the gill membranes of freshwater and salt­ water adapted killifish, Fundulus heteroclitus. For this purpose, a method for the preparation or gill plasma membranes was developed. Polyacrylarnide gel electrophoresis indicated that the two types of gills possessed many membrane proteins in common. However, the freshwater adapted group possessed a single protein not found n the saltwater adapted group and the latter contained a protein not found in the freshwater adapted group.

Phytoplankton Distribution In Waters Off The Northeastern Coast Of The United States, Sharon H. Craft

Biological Sciences Theses & Dissertations

Abstract unavailable.

Review Of: Infectious Diseases Of Wild Mammals; Parasitic Diseases Of Wild Mammals; And Infectious And Parasitic Diseases Of Wild Birds, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of: Infectious Diseases of Wild Mammals, edited by John W. Davis, Lars H. Karstad and Daniel O. Trainer; Iowa State University Press, 1970. 421 p. Parasitic Diseases of Wild Mammals, edited by John W. Davis and Roy C. Anderson; Iowa State University Press, 1971. 364 p. Infectious and Parasitic Diseases of Wild Birds, edited by John W. Davis, Roy C. Anderson. Lars Karstad and Daniel O. Trainer; Iowa State University Press, 1971. 344 p.

End Matter, Vol. 16 No. 3

Brigham Young University Science Bulletin, Biological Series

No abstract provided.

Front Matter, Vol. 16 No. 3

Brigham Young University Science Bulletin, Biological Series

No abstract provided.

86th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Forward
Administration and Staff
Nebraska Agricultural Experiment Station Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Aspects Of Reproduction In Myotis Volans, Lasionycteris Noctivagans, And Lasiurus Cinereus, Jay Dee Druecker

Biology ETDs

The reproductive cycles of Myotis volans, Lasionycteris noctivagans, and Lasiurus cinereus were investigated by examining representative sections of testes and epididymides from 254 males and serially sectioned reproductive tracts of 99 females. Specimens were collected primarily in New Mexico from late March through early October. Spermatogenesis begins in August in M. volans, October in L. noctivagans, and sometime after late September in L. cinereus. Apparently gonadotrophic secretion continues in M. volans until at least the onset of hibernation as numerous mitotic figures, small spermatogonia, and/or a few primary spermatocytes appeared in some males from September and October. During the winter, …

Mutual Exclusion Between Salmonberry And Douglas-Fir In The Coast Range Of Oregon, Kenneth Ray Still

Dissertations and Theses

One serious problem faced by the forest industry in the Pacific Northwest is poor regeneration of commercial trees on land which is harvested and subsequently dominated by brush species. In Coastal Oregon, salmonberry is one of these brush species. Detailed investigations of field sites indicate that light intensity in the brush stands was low but sufficient for germination and early growth of Douglas-fir seedlings and soil moisture percentages and nutrient levels were high enough to support early Douglas-fir growth. Laboratory tests demonstrated the presence of leachable phytotoxins in the leaves of salmonberry. The hypothesis resulting from this study is that …

Food And Feeding Habits Of The Rio Grande Mountain-Sucker, Pantosteus Plebeius (Baird And Girard), James A. White

Biology ETDs

The initial purpose of this work was to study the rela­tionship between Pantosteus plebeius (Baird and Girard), the Rio Grande mountain-sucker, and Catostomus commersoni (Lacépède ), the common white sucker, in Jemez Creek drainage, Sandoval County, New Mexico. Because insufficient informa­tion on Catostomus commersoni could be obtained, the food and feeding habits of Pantosteus plebeius were studied as an al­ternative.

Mountain-suckers were captured and observed in Jemez Creek drainage. The intestinal contents of 145 specimens were ex­amined in detail. The intestinal contents of other specimens were examined cursorily.

Pantosteus plebeius are omnivorous and they use a pair of cartilaginous jaw …

A North Temperate Bat Community: Structure And Prey Populations, Hal L. Black

Biology ETDs

Food habits and food partitioning were investigated in a North Temperate insectivorous bat community. Analysis of fecal pellets showed that all species of bats utilized moths and most species utilized beetles. There appears to be differential utilization of these two prey items. Six species of bats are moth strategists and four species are beetle strategists. In one species, Eptesicus fuscus, changes in food selection is correlated with changes in food abundance.

Food habits are discussed in relation to various parameters of the prey populations, i.e., abundance, activity, and length class diversity. Habitat partitioning is discussed in relation to type of …

The Southern Pine Beetle, Dendroctonus Frontalis Zimm, 1961-1971, Jack E. Coster

eBooks

In the last decade, since the appearance of the comprehensive reviews of southern pine beetle (SPB), Dendroctonus frontalis Zimm. (Coleoptera :Scolytidae ) *, literature by Thatcher (1960) and Dixon and Osgood (1961), much research progress on the insect has been realized. This review condenses reports of research on the insect published since 1961.

C-Reactive Protein: Potential Significance Of Quantitation In Patients With Chronic Diseases, Hajime Hayashi, Gerald A. Logrippo

Henry Ford Hospital Medical Journal

Serum C-reactive protein (CRP) levels in 351 patients with certain chronic diseases were assayed by a micro-double immunodiffusion technique developed and used in this hospital. Elevation of CRP values and severity of disease were evident in the following clinical entities: 31 of 54 patients (57%) with liver cirrhosis; 122 of 204 (60%) hepatitis patients; 34 of 52 (65%) patients with gastro-intestinal surgery; 8 of 21 (38%) uremic patients; and all of 20 patients with renal transplantation. There was good correlation between markedly elevated serum CRP values and degree of inflammation and/or physiologic stress during clinical course and recovery.

A Study Of The Seasonal Incidence Of The Arthropod & Helminth Parasites Of The Prairie Vole, Microtus Ochrogaster, & The Wood Mouse, Peromyscus Leucopus, In An Area Of Warren County, Kentucky, Richard Buckner

Masters Theses & Specialist Projects

One-hundred and fifty Microtus ochrogaster and one-hundred and fifty-five Peromyscus leucopus were examined for arthropod and helminth parasites during a study from November, 1970 through November, 1971. The mean monthly burdens, per host, of each species of parasite and observations on the seasonal incidence of each species are reported.

Nineteen species of arthropod parasites were found; these include eight species of mites, two of chiggers, one of ticks, two of lice, five of fleas, and one of hots. Most of these species exhibited very little host specificity, although there was a definite host preference. Heavier ectoparasite burdens were found on …

Phyletic Trends In Certain Lineages Of Quaternary Mammals, C. Bertrand Schultz, Lloyd G. Tanner, Lary D. Martin

Bulletin of the University of Nebraska State Museum (1924–2023)

Evolutionary trends in certain carnivores, rodents, bovids, and proboscideans are examined. Chronoclines are demonstrated for muskrats, beavers, mammoths, and bison. The Pliocene-Pleistocene boundary and Quaternary climatic fluctuations are also discussed.

The University of Nebraska State Museum contains a large stratigraphically controlled collection of Pleistocene animals. This collection provides a unique opportunity for the study of phyletic trends in certain lineages of mammals. Although a few phylogenetic lineages have been proposed (Schultz and Frankforter, 1946; Hibbard, et al., 1965), this has not been done for the vast majority of Pleistocene mammals. However, there is much more data available than this would …

Yields From 18-Year-Old Aspen Via Total Tree Harvesting, M. K. Benson, D. W. Einspahr

Aspen Bibliography

Described are the results of harvesting the above-ground parts of an 18-year-old aspen stand and the observations made on the initial suckering of the new stand. The yields for the stand of 17.7 cunits per acre were greater than predicted yields of comparable material for the site at age 35. Suckering on the area the first year after cutting averaged 37,000 aspen stands per acre and 2.6 feet in height.

Factors Affecting Calcification Processes In The Hermatypic Corals Pocillopora Damicornis And Porites Compressa, Conrad D. Clausen

Loma Linda University Electronic Theses, Dissertations & Projects

Temperature affects coral distribution and it also affects rates of physiological processes. Little attention has been given through, to the relation between the basic effect of temperature on reaction rates at the molecular level and its effect on coral distribution at the organismic level. Incubating coral samples in sea water containing ⁴⁵Ca incorporation as a measure of calcification rate, I studied the effects of temperature on calcification in two coral species, P. damicornis and P. compressa. CaCO₃ deposition was chosen because of its basic importance in reef formation. Immediate effects of temperature on calcification rates were studied, as …

The Comparative Histochemistry Of The Infective Oncospheres Of Oochoristica Osheroffi Meggitt, 1934, And Oochoristica Scelopori Voge And Fox, 1950, (Cyclophyllidea: Linstowiidae) With Notes On The Origins Of The Egg Membranes, Gary R. Coulter

Loma Linda University Electronic Theses, Dissertations & Projects

Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.

Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.

Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was …

The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady

Loma Linda University Electronic Theses, Dissertations & Projects

Triatoma protracta protracta, Triatoma riibida rubIda, and two strains of Rhodnius prolixus were successfully reared germfree into the fourth generation. Triatominae eggs were disinfected externally with iodine and the nymphs reared in vinyl plastic germfree modules. Germfree mice were used as the host. This is the first report of Triatominae having been reared in a germfree environment. These bugs were never exposed to bacteria internally or externally to the fourth generation.

Comparisons of conventional control bugs with replicate germfree groups in the first two generations revealed but slight differences in developmental periods. The mean adult longevity of all …

The Prairie Naturalist Volume 4, No. 2. June 1972

The Prairie Naturalist

EDITORIAL: Coordination: A Step for Environmental Protection ▪ Paul B. Kannowski

AN ANALYSIS OF BARN OWL AND GREAT HORNED OWL PELLETS FROM WESTERN NEBRASKA ▪ Eric A. Rickart

SUMMER OBSERVATIONS OF THE SHORT-EARED OWL IN THE RED RIVER VALLEY ▪ W. J. Walley

ADDITIONAL GLAUCOUS GULL RECORD FOR NORTH DAKOTA ▪ B. J. Rose

MIGRATION OF BIRDS IN NORTH DAKOTA DURING FALL 1971 ▪ Douglas H. Johnson, John T. Lokemoen and Paul F. Springer

WIDGEONGRASS BALLS ON ALKALI LAKES ▪ George A. Swanson and Paul F. Springer

A PROBABLE INSTANCE OF SONGBIRD COLLISION MORTALITY ▪ R. Wayne Weier

MUNICIPAL FLOODPLAIN …