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90th Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station
Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

The Concentrations Of Ten Heavy Metals In Some Selected Lake Powell Game Fishes, Robert E. Bussey

Biology ETDs

Ten tissues from each of four species of fish-large­mouth bass (Micropterus salmoides), black crappie (Pomoxis nigromaculatus), walleye (Stizostedion vitreum) and rainbow trout (Salmo gairdneri) from Lake Powell were analyzed and compared for the concentrations of ten heavy metals: Fe, Ca, Mg, Cu, Cr, Cd, Zn, As, Se, and Pb.

Samples were digested with nitric and perchloric acids, and analyses were performed by atomic absorption spectro­photometry. Concentrations were expressed on a dry weight basis for all tissues, and were not corrected for percent recovery.

As expected, high levels of iron were observed in the blood tissues, with low concentrations being observed …

A Comparison Of The In Vitro Phagocytic Activity Of Polymorphonuclear Leucocytes And Alveolar Macrophages From Man And Rabbits, Thomas S. Harrison

Biology ETDs

It has been thought for many years that the alveolar macrophage is the primary agent responsible for prevention or bacterial invasion or the lung. Numerous studies with Staphylococcus aureua, Staphylococcus epidermidis, Escherichia coli. Klebsiella, and Proteus have been cited to substantiate this statement. Together these organisms cause less than ten per cent of human bacterial pneumonias. Both in vivo and in vitro studies have shown that the alveolar macrophage is capable of bacterial clearance with time. Sadly lacking ss evidence that alveolar macrophages readily phagocytose Streptococcus pneumoniae which are responsible tor greater than ninety percent of all bacterial pneumonias. The …

Macroinvertebrates And Diatoms On Submerged Bottom Substrates, Lake Powell, Ellen T. Louderbough

Biology ETDs

To collect and examine the organisms associated with newly inundated terrestrial vegetation in Lake Powell, small plastic Christmas trees were "planted" along a sloping sandy bottom at depths of 2, 3, 4.5, 6, 8, and 10 meters. For the 17 months from July, 1973, through October, 1974, the trees were harvested and replanted by scuba divers at 4- and 12-week intervals. The macroinvertebrate and diatom components of the periphytic community associated with each tree were collected and evaluated for number of individuals per unit area (density) and species composition. A diversity index was computed for the diatom portion of each …

Influence Of Light On Algal Growth In The Lower Willamette River, Stephen Arthur Wille

Dissertations and Theses

During the summer of 1974 chemical conditions in the lower reaches of the Willamette River, Oregon were similar to those in other rivers currently experiencing nuisance algal growth problems. Temperature and chemical nutrients are not limiting. Relatively high populations of phytoplankton and productivity values for upstream periphyton beds and surface waters suggest moderately eutrophic conditions. However, with increased depth in the lower river, and a constant euphotic zone, the amount of photosynthetically available light is reduced. With sufficient depth and complete mixing the critical depth is exceeded. Primary productivity rates are subsequently limited by low light availability in the lower …

Animals Suffer For Science, Henry Spira

Articles

No abstract provided.

Allografts And Xenografts Of Thyroid Gland Tissue And Cultured Thyroid Gland Cells Into Thyroidectomized Rats, Jerry Martin Katz

Biology ETDs

The most desirable way of treating endocrine deficiencies would be by the successful transplantation of functional endocrine tissue into the deficient animal. However, unless the donor and recipient are identical twins or from an inbred strain of animals, an immune response is usually elicited in the recipient by the foreign tissues leading to rejection of the transplant. Thyroid gland cells which have been enzymatically dispersed from thyroid tissue and cultured in vitro are capable of reforming functional thyroid tissue when implanted into animals of the same strain. The purpose of this work was to test the ability of cultured thyroid …

Effects Of Acute Dehydration, Heat Acclimatization And Physical Condition On Fluid Shifts Induced By Lower Body Negative Pressure, Michael Dyar Venters

Biology ETDs

This study was designed to analyze the effects of heat acclimatization, acute dehydration and physical conditioning on the fluid shifts induced by lower body negative pressure (LBNP). Ten male subjects, five trained runners and five non-runners, were subjected to LBNP before and after nine days of acclimatization to work in the heat and pre- and post-dehydration induced by heat and exercise. During each of the four LBNP runs the subjects were monitored for changes in blood volume and variances in the volume of the left forearm and lower leg at the calf. Each LBNP test consisted of five minute long …

Cardiorespiratory Function During Two Methods Of Lung Lavage And During Unilateral Hypoxia, Steven Allen Silbaugh

Biology ETDs

Therapeutic lung lavage is a procedure by which the interior of the lung of a living animal is “washed” with a suitable solution. Broncho­pulmonary lavage, a special type of lung lavage in which the bronchioles and alveoli are washed, is the major concern of this thesis. Broncho­pulmonary lavage was investigated in 1920 as a possible treatment for lungs damaged by irritant gases and in the late 1950‘s as a method of hemodialysis. Currently, it is effectively used for the treatment of patients with obstructive lung disease and for the removal of radioactive materials from the mammalian lung. Bronchopulmonary lavage interferes …

Endoparasites Of Cats In Two Central Utah Communities, Thomas W. Sawyer

Theses and Dissertations

A survey of the endoparasites of 100 stray cats in central Utah was conducted. Each cat was euthanized, and the entire alimentary tract, gall bladder, bile duct, heart, respiratory and excretory systems were excised and examined for endoparasites. Fecal samples were examined by a sugar flotation technique. Parasites identified and number of cats infected were: Isospora felis 5; I. rivolta, 4; Dipylidium caninum, 1; Hydatigera taeniaformis, 10; Toxocara mystax, 43; and Physaloptera sp., 2. One ectoparasite, Cheyletiella parasitivorax, was identified in the feces of 4 cats. Dipylidium caninum and T. mystax are known to cause accidental infection in humans and …

The Wavelength Dependency Of Ultraviolet Induction Of Simian Virus 40 From A Transformed Line Of Baby Hamster Kidney Cells, Timothy Bichenbrenner

Masters Theses & Specialist Projects

For our investigation, an established line (E line) of baby hamster kidney cells which was transformed by the incorporation of Simian Virus 40 into the cellular genom was used. The induction of this virion by irradiating these cells with ultraviolet light was studied. The purpose of this thesis was to determine the wavelength dependency (at four selected ultraviolet mercury emission lines) of the induction of the SV40 genome from these cells. The viral titres of induction were measured by the agar overlay plaque assay method and by the fluorescent antibody microassay technique.

At the four wavelengths tested 265 nm and …

Autecological & Populational Investigation Of Carpinus Caroliniana Walt., Gordon Wardell

Masters Theses & Specialist Projects

Analysis of habitats containing Carpinus caroliniana in two distinct physiographic regions of Kentucky revealed that this species has a wide range of tolerance to light intensities and soil pH. Species most commonly associated with Carpinus within the two physiographic regions were Platanus occidentalis, Acer saccharum, Liquidambar styraciflua, Fraxinus spp. and Fagus grandifolia.

Morphological and anatomical investigations of populations representing the geographic distribution of Carpinus caroliniana in the United States indicated that the more northern population had heavier fruit, larger bracts, and secondary wood with higher specific gravity.

Growth chamber experiments involving dormant buds collected from natural habitats indicated differences …

The Isolation And Identification Of Yeast-Like Fungi In Non-Active Tuberculosis Patients In Rowan County, Kentucky, Connie Harris Baker

Morehead State Theses and Dissertations

A thesis submitted to Morehead State University in partial fulfillment of the requirements for the degree of Master of Science in Biology by Connie Harris Baker in July of 1976.

Constitutive Nature Of 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Vahag Marootkhanian

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NADP⁺ oxidoreductase; E.C.I.I.I.69.) of Gluconoacter suboxydans was examined and determined to be constitutive in nature. Crude, cell -free enzyme extracts were prepared by sonication of cells grown on 2.0'% glucose and 2.0% ethanol in various experiments. The specific activity of 5-ketogluconate reductase in the crude enzyme extracts of cells grown under various conditions was determined. The specific activity remained relatively constant strongly indicating the constitutive nature of the enzyme.

Electrophoretic Heterogeneity Of Nicotinamide Adenine Dinucleotide Phosphate-Dependent 5-Ketogluconate Reductase Of Gluconobacter Suboxydans, Sharon Dianis

Masters Theses & Specialist Projects

NADP⁺-dependent 5-ketogluconate reductase (D-Gluconate:NAD(P)⁺ oxidoreductase E.C. 1.1.1.69) isozymes were detected in cell-free extracts of Gluconobacter suboxydans (American Type Cultures Collection, Bethesda, Maryland, strain 621). Cell-free enzyme extracts were prepared from cells grown in media which contained glucose, gluconate, glycerol, sorbitol, or mannitol as the sole carbon source in 2% concentration. The extracts were fractionated using analytical polyacrylamide disc-gel electrophoresis. Gels were stained specifically for 5-ketogluconate reductase. Three isozymes were present in each extract from cells grown on the five different carbon sources. Gels stained in a reaction mixture which contained glycerol exhibited two of the isozymes.

An …

Radioprotection Of Oral Cavity Structures By S-2-(3-Aminopropylamino) Ethyl Phosphorothioate (Wr-2721), Ronald King

Masters Theses & Specialist Projects

Studies reporting a high concentration of WR-2721 in mouse salivary glands led to our studies of possible radioprotection of these glands by this drug from ionizing radiation. Oral effects of radiation in the presence of WR-2721 were studied in mice and dogs. Histological evaluation of mouse salivary glands irradiated with 1000 rads of ⁶⁰Co showed essentially no difference between control and experimental animals. Almost full regeneration of the serous salivary components occurred by 6 months in both groups and neither group had changes in the mucous glands. The use of higher doses of radiation in the mouse was prevented …

Anatomy Of The Adults Of Loxagrotis Albicosta, C.Y. Oseto, T.J. Helms

University Studies (University of Nebraska) (1888–1984)

LEPIDOPTERA are divided into the major suborders Monotrysia and Ditrysia (Borner, 1939). The vast majority of Lepidoptera belong to the Ditrysia. The Noctuidae, which is the largest family in the order, is probably the most destructive (Metcalf, et aI., 1962). It includes Loxagrotis albicosta (Smith), the western bean cutworm, originally a pest of field beans, which has become a relatively serious pest of corn in southwestern and central Nebraska (Hagen and Roselle, 1972). Adults emerge from early July to late August, and eggs, which are deposited on the upper sides of corn leaves, hatch in approximately I week. The newly …

South Dakota Farm And Home Research, South Dakota State University

South Dakota Farm and Home Research: 1949 -1998

Alfalfa Haylage Gains from Addition of Dried Whey [p] 3
The Back Side Makes a Difference [p] 6
Spotting the Protein in Bread Wheats [p] 8
Out There Waiting for You [p] 11
Why Some Manpower Programs Fail [p] 13

The Relationship Between Body Fat And Physical Well-Being Of Children, Aged Six To Thirteen, As Determined By Physical Activity And Physical Fitness, Paul Sieczkowski

Student Work

Body fat has several implications for an individual's physical well-being. Aside from its functional aspects as an energy reserve and as a thermal insulator, excess body fat has been related to an increased mortality rate (Dublin, 1930). Vitale (1973) and deVries (1974) emphasized that excess fat is associated with many degenerative diseases such as diabetes, gallstones, high blood pressure, and heart disease. Mayer (1953) noted that there might be seasonal changes in the body fat level due to changes in activity level. Johnson, Burke,, and Mayer (1956) traced the onset of obesity of most "late obesity" children to the winter …

Final Report - Lake Mead Monitoring Program, James E. Deacon

Publications (WR)

The Lake Mead monitoring program has developed a substantial body of information on the physical, chemical and biological limnology of Lake Mead since 1972. This report summarizes pertinent aspects of that data, with emphasis on studies completed in 1975 -76. It is our continuing hope that the data developed by us will be useful to an ever broader group of users of the water resource represented by Lake Mead. We have been privileged to see our data have a significant influence in some very important water resource decisions over the past four years. There is every reason to expect that …

Studies Of The Purification Of Nad-Dependent And Nadp-Dependent Isocitrate Dehydrogenase By General Ligand Affinity Chromatography, Geoffrey H. Bertkau

Chemistry & Biochemistry Theses & Dissertations

Purification of Yeast NAD⁺-dependent isocitrate dehydrogenase was partially successful when utilizing an NAD⁺-ribosyl-hydrazide Sepharose affinity chromatography column. Although no specific elution system was found, a maximum purification of sixty-five fold was achieved with 0.5M KCl elution. A 38-fold purification was obtained when 20mM NAD⁺ was employed for elution.

Yeast and calf liver NADP⁺-dependent isocitrate dehydrogenase were also found to bind to NAD⁺-ribosyl-hydrazide Sepharose as well as the NAD⁺-dependent enzyme. Again, elution was achieved with either o,5M KCl or 20 mM NADP⁺.

Ammonium sulfate fractionation decreased the yield …

Kinetic And Binding Studies On L-∝-Glycerophosphate Dehydrogenase, Paul Richard Rosevear

Chemistry & Biochemistry Theses & Dissertations

The properties of the coenzyme, NAD, binding site of chicken muscle L-∝-glycerophosphate dehydrogenase were studied. Adenosine monophosphate, adenosine diphosphate and adenosine diphosphoribose were sha.vn to inhibit the enzyme competitively with respect to NAD. The presence of adenosine, pyrophosphate and ribose regions in the coenzyme binding site of the enzyme was suggested by the inhibitor constants obtained for these compounds.

Disodium monoalkyl phosphates, n-butyl to n-dodecyl phosphate, inclusive, were also shown to inhibit the L-∝-glycerophosphate dehydrogenase catalized reaction competitively with respect to NAD. A positive chain length effect was observed in the binding of these compounds to the enzyme, suggesting the …

Sex Pheromone Activity In The Foveal Glands In The Two Species Of Ixodid Ticks: Dermacentor Andersoni (Stiles) And Dermacentor Variabilis (Say), Lynn Anne Collins

Biological Sciences Theses & Dissertations

Sex pheromone activity in the foveal glands of female Dermacentor andersoni (Stiles) and Dermacentor variabilis (Say) is described. Autoradiography revealed the accumulation of ³⁶Cl in the foveal glands of females during their first 120 hours of feeding on a ³⁶Cl-labeled host. Emissions collected from these females contained a ³⁶Cl-labeled product. This product, however, was absent in emissions collected from the same females with their foveae dorsales obstructed.

Accumulation of ³⁶Cl in the foveal gland, in conjunction with the release of a ³⁶Cl-labeled product through the foveae dorsales, suggest localization of sex pheromone activity within these …

An Intensive Study Of The Spring Phytoplankton Bloom In Lynnhaven Bay, Virginia, Donald Lynn Webster

Biological Sciences Theses & Dissertations

Phytoplankton samples were collected biweekly from two stations, one in each branch of Lynnhaven Bay, Virginia over the period January 24 to June 9, 1975. Water temperature, salinity, pH, oxygen, ammonia, urea, reactive nitrite, reactive nitrate, reactive phosphorus, productivity, transparency, and weather conditions were recorded concurrently. Data were manipulated employing analysis of variance, simple correlation, correlation matrices, and forward stepwise multiple regression. Total phytoplankton numbers exhibited a unimodal pattern. A pre-bloom pulse was recorded at both stations in late February. The spring bloom lasted from April through May. Phytoplankton growth was primarily a function of nutrient supply and temperature. Nutrient …

Structural Comparisons Of Four Plant Communities In The Great Dismal Swamp, Claire Virginia Dabel

Biological Sciences Theses & Dissertations

Four plant communities in the Great Dismal Swamp, located on the coastal plain in southeastern Virginia, were sampled and estimates of density, basal area and aboveground standing crop biomass were determined, Biomass was estimated as 229,800 kg/ha in the Chamaecyparis thyoides community and as 344,500 kg/ha in the Taxodium distichum community. Biomass for the two hardwood communities, the

Acer-Nyssa community and mixed hardwood (Quercus-Nyssa-Acer-Liriodendron-Liquidamber) community was estimated as 195,700 kg/ha and 194,600 kg/ha respectively and did not differ significantly (P

An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton

Biological Sciences Theses & Dissertations

Concentrations of cAMP increased with time in pea hypocotyls assayed at 24 hour intervals over a period of four days. The highest concentration was 20.1 pmoles/mg protein which occurred in 96 hour hypocotyl tips. Ninety-six hour root bodies (dissected region between tip and embryo) achieved a concentration of 8.6 pmoles/mg. Concentrations of cAMP in embryos remained constant around one pmole/mg for three days and values doubled on the fourth day.

Hypocotyls, after germination for 72 hours, were dissected and incubated for various lengths of time in water and 3xl0^-8M IAA. Tips incubated for two hours showed a tenfold …

Presumpscot River Basin - Cumberland County Tidewater Water Quality Management Plan, Bureau Of Water Quality Control

Maine Collection

Presumpscot River Basin - Cumberland County Tidewater Water Quality Management Plan

Maine Department of Environmental Protection, Bureau of Water Quality Control, Division of Water Quality Evaluation and Planning, August, Maine (June 1976).

"Prepared pursuant to Section 303 (e) of the Federal Water Pollution Act Amendments of 1972"

Contents: Letter of Transmittal / Table of Contents / Appendices and Tables / I. Summary, Conclusions, Recommendations / II. Introduction / III. Water Quality / IV. Planning Activities / Appendices / Tables

Front Matter, Vol. 20 No. 4

Brigham Young University Science Bulletin, Biological Series

No abstract provided.

End Matter, Vol. 20 No. 4

Brigham Young University Science Bulletin, Biological Series

No abstract provided.

Whole Issue Nebraska Bird Review (June 1976) 44(2)

Nebraska Bird Review

Table of Contents

The Seventy-fifth Annual Meeting ..................................... 18

1975 (Eighteenth) Fall Occurrence Report ........................... 19

Brown Towhee Reported ................................. 30

Book Reviews........................................ 31

Notes ................................................ 33