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Microheterogeneity Of Type Ii Camp-Dependent Protein Kinase In Various Mammalian Species And Tissues, Alison M. Robinson-Steiner, Stephen J. Beebe, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Excluding autophosphorylated species, at least six forms of the regulatory subunit of type II cAMP-dependent protein kinase (R(II)) from various mammalian tissues were identified by sodium dodecyl sulfate (SDS) gel electrophoresis of purified samples and of crude preparations photoaffinity labeled with 8-azido[32P] cAMP and by gel filtration. After autophosphorylation some heart R(II) forms termed type IIA (bovine, porcine, equine, and dog) shifted to a more slowly migrating band on SDS gels while others termed type IIB (rat, guinea pig, rabbit, and monkey) did not detectably shift. Both subclasses of R(II) exhibited variation in apparent M(r) on SDS gels. Bovine and …

Two Classes Of Camp Analogs Which Are Selective For The Two Different Camp-Binding Sites Of Type Ii Protein Kinase Demonstrate Synergism When Added Together To Intact Adipocytes, Stephen J. Beebe, Rob Holloway, Stephen R. Rannels, Jackie D. Corbin

Bioelectrics Publications

Twenty-five cyclic nucleotide analogs were tested individually to act as lipolytic agents and to activate adipocyte protein kinase. The lipolytic potency of individual analogs correlated better with their K(a) for protein kinase and their lipophilicity rather than with either parameters alone. Some of the most potent lipolytic analogs had high I50 values for the particulate low K(m) cAMP phosphodiesterase suggesting that their effect was not due to raising endogenous cAMP levels through inhibition of phosphodiesterase. The most potent lipolytic analogs contained a thio moiety at the C-8 or C-6 position. These analogs exhibited concave upward dose-response curves. At high concentrations …

Systematics And Palynology Of Picrodendron Further Evidence For Relationship With The Oldfieldioideae (Euphorbiaceae), W. John Hayden, W. T. Gillis, D. E. Stone, C. R. Broome, G. L. Webster

Biology Faculty Publications

Although known to botanical science for 285 years, the genus Picrodendron Planchon has been poorly understood for most of this time. The most pervasive problem has been that of discerning familial relationships, and there have been additional difficulties in typifying the generic name (Hayden & Reveal, 1980) and in distinguishing its three nominate species. This paper provides a systematic treatment for Picrodendron and demonstrates its relationships with Euphorbiaceae subfam. Oldfieldioideae Kohler & Webster as evidenced by data on gross morphology, palynology, anatomy, and cytology.

Leptodactylus Podicipinus (Cope, 1862) Y Leptodactylus Chaquensis Cei, 1959 (Anura, Leptodactylidae), En El Uruguay, Raúl Vaz-Ferreira, Rafael O. De Sá, Federico Achaval, Annelise Gebrau

Biology Faculty Publications

En base al estudio de las colecciones obtenidas principalmente en excursiones a la zona NO de Uruguay con motivo del relevamiento faunístico del área de Salto Grande, se avanzó considerablemente en el conocimiento de la distribución de Leptodactylus podicipinus (Cope, 1862) y Leptodactylus chaquensis (Cei, 1950), en nuestro país.

Se cita el material estudiado y las observaciones de campo realizadas; se dan los caracteres generales de ambas especies, se describen los hábitats, y se tratan las características de las vocalizaciones.

Curatorial News From The University Of Richmond Herbarium, W. John Hayden

Biology Faculty Publications

The Department of Biology at the University of Richmond maintains a herbarium of approximately 15,000 specimens. In an effort to make this collection better known to Virginia botanists, this note describes some recent curatorial improvements upon the UR Herbarium and outlines its contents.

A Study Of Cladonia Cryptochlorophaea And Morphologically Similar Species In Illinois, Indiana, And Wisconsin, Scott S. Wilcer

Masters Theses

Collections of Cladonias with cup-shaped podetia were made in Illinois, Indiana, and Wisconsin in order to determine the distribution of an atranorin-containing variant of Cladonia cryptochlorophaea, which was discovered in 1982 in Coles County, Illinois by Cheryl Cunningham. A second objective was a distributional study of the Cladonias having cup-shaped podetia in the same three state area.

A total of 591 specimens were collected from the three states. The collection areas for Illinois were: Jackson Hollow in Pope County, Giant City State Park and Touch of Nature Interpretive Center in Jackson and Williamson Counties, Starved Rock State Park in …

Woody Vegetation Of Baber Woods: Composition And Changes Since 1964, James Alvin Newman Jr.

Masters Theses

A comparative study of the woody vegetation changes at Baber Woods, Edgar County, Illinois, since 1964 was completed in 1983. This 51 acre woodlot is a remnant of a much larger forest which once occuppied the Shelbyville Moraine.

An inventory of the woody vegetation shows that the present stand consists of 109 stems per acre with a basal area of 113 sq. ft. per acre. In this survey, 33 woody species were found. Sugar maple had the highest Importance Value (IV) of all woody species present, having the highest density in both the 4-6 and 7-12 inch diameter classes, as …

Comparison Of Surface Polysaccharides Of R. Leguminosarum Anu57 (Exo-1), R. Leg. Anu57(Pbr1an) (Nod+, Nif-), And R. Leg. Anu57(Pjb5j1) (Nod+, Nif-), Frank G. Hustmyer

Masters Theses

Extracellular polysaccharides (EPS) and lipopolysaccharides (LPS) are suspected to be involved in the legume/Rhizobium symbiotic process. Polysaccharide compositions from the EPS, LPS, capsular polysaccharide (CPS), and small polysaccharide (SmPS) were compared between Rhizobium leguminosarum ANU57 (parent, Exo^-1) and two mutants R. leg. ANU57(pBR1AN) (nod⁺, nif^-) and R. leg. ANU57(pJB5J1) (nod⁺, nif^-) both containing a Sym (symbiotic) plasmid insertion. R. leg. ANU57(pBR1AN) and R. leg. ANU57(pJB5J1) are nod⁺ on clover and peas, respectively. The nod⁺ mutants produce larger amounts of EPS and decreased amounts of LPS compared to …

Avoidance Learning And Memory In Largemouth Bass (Micropterus Salmoides) Fed Bufo Tadpoles, Randy John Gawlik

Masters Theses

Tadpoles of the American toad (Bufo americanus) have been shown to be distasteful to largemouth bass (Micropterus salmoides) predators who learn, on the short term, to avoid them. How long bass retain this learned avoidance of Bufo larvae is unknown so an experimental design was constructed in an attempt to answer this question.

Largemouth bass were divided into four treatment groups. Two groups were initially fed exclusively larvae of Bufo americanus (one group was fed large tadpoles, the other group fed small larvae). Similarily, the other two groups were initially fed spring peeper (Hyla crucifer …

Seasonal Lekking Behavior Of The Greater Prairie-Chicken In Illinois, Scott A. Simpson

Masters Theses

Spring behavior of prairie-chickens (Tympanuchus cupido) has been studied extensively, however there is little information on fall-winter lekking activity. Greater prairie-chickens were observed weekly on a lek from 25 September 1982 to 23 June 1983 during morning and evening hours in Jasper County, Illinois. The peak number of prairie-chicken males on the lek occurred in February although hen attendance did not occur until 10 March 1983. All spring lekking activities were higher compared to that of the fall-winter period. Fall-winter lek activity was aggression which centered on probably brief, less intense male aggression which probably establishes or maintains …

Observations On Relationships Between Limb Measurements And Mode Of Locomotion In Malaysian Turtles, Duayne Nyckel

Masters Theses

Twelve Genera (spanning three Families) of Malaysian Turtles were studied to determine whether bone lengths could be correlated to mode of locomotion. Ulna, humerus, tibia, femur, coracoid (among others) were measured and results were presented graphically. Five of the genera were observed singly in a large aquarium. Data were recorded on the amount of time each spent in different locomotor modes. Both limb length data and aquarium observations suggest that turtles can be grouped into three main locomotor habits (i.e. terrestrial walking, bottomwalking, and swimming). The terrestrial group includes the genera, Geochelone, Kinixys, and Gopherus. Bottomwalkers include …

Vegetative Key To Grasses Of The Sand Hills Region Of Nebraska, David M. Sutherland

Biology Faculty Publications

All illustrated key is provided that will facilitate identification of grasses in the vegetative condition in the Sand Hills region of Nebraska. The key separates 97 species, varieties, and species groups and discusses or partially separates 14 additional taxa. It emphasizes characteristics that may be observed in the field with a hand lens and is illustrated with 109 camera lucida drawings.

Wood Anatomy Of Hydrophyllaceae. Ii. Genera Other Than Eriodictyon, With Comments On Parenchyma Bands Containing Vessels With Large Pits, Sherwin Carlquist, Vincent M. Eckhart

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Floral Pigments And Nectar Constituents In The Genus Puya (Bromeliaceae), Ron Scogin, C. Edward Freeman

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Las Vegas Wash Advanced Water Quality Study: Final Report, Richard A. Roline, James J. Sartoris, U.S. Bureau Of Reclamation

Publications (WR)

The purpose of the Las Vegas Wash Advanced Water Quality Study is to determine the existence, extent, and mechanisms of nutrient and toxin stripping in Las Vegas Wash under present conditions and under future conditions, both with and without construction of the proposed salinity control unit as described by the Bureau of Reclamation (USBR, 1982b). This study was performed for the Lower Colorado Region Division of Planning by personnel of the Environmental Sciences Section of the Division of Research and Laboratory Services, E&R Center, Denver, Colorado. Work on the study began in February 1983. The general approach adopted for this …

Factors Affecting Reproductive Success Of Bonytail Chubs And Razorback Suckers In Lake Mohave, Michael A. Bozek, Larry J. Paulson, James E. Deacon, U.S. Department Of Interior, Fish And Wildlife Service

Publications (WR)

Razorback suckers (Xyrauchen texanus) and bonytail chubs (Gila elegans) were once widespread throughout the Colorado River system. The ranges and populations of these native species and others have declined in the past 50 years. Bonytail chubs appear to be extremely rare and possibly nearing extinction in the Upper Colorado River Basin. The razorback sucker is widely distributed in the upper river but is considered rare throughout most of that range and is also rare in the Grand Canyon. Razorback suckers are abundant only in a few habitats in the upper river. They congregate and spawn over …

The Reproductive Biology Of Sternotherus Minor Minor (Reptilia: Testudines: Kinosternidae) From The Southern Part Of Its Range In Central Florida, Cory R. Etchberger

Retrospective Theses and Dissertations

Variation in chelonian reproductive patterns is well documented. Previous studies of loggerhead musk turtles (Sternotherus minor) reproduction have not attempted to control for variation in latitude, local population differences, and seasonal variation. The present study attempts to control for these variables by collecting turtles from one population for one reproductive season. The reproductive pattern of S. minor at the southern limit of its range (Central Florida) is compared to those of S. minor studied elsewhere. Both male and female musk turtles mature after five to six years and at approximately 60mm plastron length. No sexual dimorphism in overall body size …

The Effects Of Beach Restoration On Marine Turtles' Nesting In South Brevard County, Florida, Paul W. Raymond

Retrospective Theses and Dissertations

The south Brevard coast of Florida is a major nesting ground for the Atlantic loggerhead turtle (Caretta caretta). A beach restoration project was completed in the winter of 1980-81 at Indialantic and Melbourne Beach. A study was conducted to investigate the effects of beach restoration on marine turtle nesting during the summer months of 1981 and 1982. A 3.1 km restored beach study area and two control beach study areas, each 3.1 km in length and consisting of natural beach sands, were established. The entire study area (9.3 km) was monitored for nesting and non-nesting emergences (false crawls) and a …

The Isolation And Identification Of Flavonoids In Zygocactus, Jacqueline M. Wade

Retrospective Theses and Dissertations

No abstract provided.

Factors That Influence Plant Species Richness On Habitat Islands Of Sand Pine Scrub, Cindy B. Connery

Retrospective Theses and Dissertations

No abstract provided.

Comparison Of Three Methods For Sampling Fishes And Macroinvertebrates In A Vegetated Wetland, B. J. Freeman, H. S. Greening, J. Douglas Oliver

Faculty Publications and Presentations

Three methods of sampling fishes, two seining methods and a drop trap method, were evaluated in heavily vegetated freshwater habitats. The portable drop trap method, which utilized a 1 x 1 m-sq. trap, collected significantly more macroinvertebrates and fish per unit area than did the seining methods. The meter square drop trap offered the additional advantages of a greater number of animals per unit effort and an integrated sample of vegetation, macroinvertebrates and fish in a given area. A 90% (s.d.= 7.4%) recovery of tagged fishes released into the traps in different habitats showed the m2 drop trap to be …

Flora And Early Secondary Succession In Wetlands Of The Lower Kanawha River Floodplain, West Virginia, Brenda Kay West

Theses, Dissertations and Capstones

Vegetational and floristic analyses were performed on eight wetlands undergoing early secondary succession on the Kanawha River floodplain of' West Virginia. All sites have been cleared previously and have been revegetated for 30 years or less. Vegetation was classified into ten cover types according to species composition and physiognomy. Aquatic, graminoid dominated wet meadow, shrub-wet meadow, Spiraea tomentosa dominated, Comus amomum dominated, and various early forested wetlands were among the cover types classified and surveyed. Frequency, density, dominance, and importance values were established with random plot sampling for both herbaceous and woody species. A total of 262 species distributed among …

Activation Of B Lymphocytes By Monovalent Anti-Lyb-2 Antibodies, Subbarao Bondada, D. E. Mosier

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Small resting B lymphocytes are activated when their cell surface immuno-globulin (Ig) receptors interact with specific antigen or anti-Ig antibodies. Such a receptor-ligand interaction leads to enlargement and proliferation of B lymphocytes. These activated B cells can subsequently mature into Ig-secreting cells in the presence of appropriate accessory cell signals (1-3). However, monovalent anti-Ig antibodies cannot induce B cell activation even though they can bind to the surface Ig (4-7). It has thus been concluded that membrane Ig has to be cross-linked to induce early steps in B cell activation (membrane depolarization; 8) as well as later steps (proliferation). T …

Growth Analysis Based On Degree Days, M. P. Russelle, Wallace Wilhelm, R. A. Olson, James F. Power

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Comparisons of growth analysis functions within and among experiments are often confounded by sources of variation other than those imposed by treatment. we suggest use of a temperature index, such as modified growing degree days, as the divisor in growth functions to facilitate treatment comparisons within certain experiments and to reduce the effects of differing temperature regimes among experiments on these comparisons. Three experiments were identified to provide data to analyze this new approach. Mean absolute growth rate (GR) and mean relative growth rate (RGR) were compared in two experiments with maize (Zea mays L.) conducted in eastern Nebraska. …

Endocrine Mechanisms Of Puberty In Heifers: Estradiol Negative Feedback Regulation Of Luteinizing Hormone Secretion, M. L. Day, K. Imakawa, M. Garcia-Winder, D. D. Zalesky, B. D. Schanbacher, Roger J. Kittok, J. E. Kinder

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The hypothesis that luteinizing hormone (LH) secretion in prepubertal females is responsive to estradiol negative feedback and that decreased feedback occurs as puberty approaches was tested in heifers. In the first experiment, seven heifers were maintained prepubertal by dietary energy restriction until 508 days of age (Day 0). All heifers were placed on a high-energy diet on Day 0 at which time they received no additional treatment (CONT), were ovariectomized (OVX) or were ovariectomized and subcutaneously implanted with estradiol-17β (OVX-E₂). This feeding regimen was used to synchronize reproductive state in all heifers. A second experiment was performed with …

Characteristics Of A Ca++ Atpase Enzyme In The Epidermis Of Molting Blue Crabs, Callinectes Sapidus, Joanne Elizabeth Lapetina

Master's Theses

Epidermal Ca⁺⁺-activated ATPase was investigated in relation to the molt cycle of the blue crab, Callinectes sapidus. Premolt and postmolt tissue samples demonstrated significant elevations in Ca⁺⁺-activated ATPase activity in comparison to intermolt tissue samples. These results support the suggestion that this enzyme may participate in demineralization of the old cuticle, as well as calcification of the new cuticle.

The enzyme is localized in the heavy particulate-nuclear fraction and is distinguished from mitochondrial Ca⁺⁺ ATPase found in other tissues by differences in sensitivity to mersalyl acid. 0 The enzyme is most active at 49 C …

Enteric Redmouth Disease Of Salmonids, G. L. Bullock

United States Fish and Wildlife Service: Publications

Enteric redmouth disease (ERM) is a systemic bacterial infection of fishes, but is principally known for its occurrence in rainbow trout, Salmo gairdneri. The disease was first reported in the 1950's by Rucker (1966), and in recognition of that fact, the causal organism was named Yersinia ruckeri by Ewing et al. (1978). Although Wagner and Perkins (1952) described a bacterial disease that produced a red-mouth condition in rainbow trout in California and Colorado, it was caused by a different bacterium; they isolated Pseudomonas hydrophila rather than Y. ruckeri.

Two Purified Rnas Of Soil-Borne Wheat Mosaic Virus Are Needed For Infection, Yukio Shirako, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

RNAs of soil-borne wheat mosaic virus (SBWMV) from virions 281 nm, 138 nm and 92 nm long (designated here by relative lengths as 1∙0L, 0∙SL and 0∙35L, respectively), were isolated and purified by three cycles of sucrose density gradient centrifugation. Infectivity assays with these RNAs proved the bipartite nature of SBWMV, the combination of 1∙0L and either 0∙5L or 0∙35L RNAs being required for infection and for multiplication of progeny viruses. The 0∙5L RNA underwent deletion mutation, producing smaller variants with various sizes, of which 0∙4L and 0∙35L RNAs were confirmed to be functional in combination with 1∙0L RNA. The …

The Effects Of Calcium Hydroxide Treatment Of Sunflower Seeds On Ration Digestibility And Rumen Parameters In Holstein Steers, Ann C. Spearow

Electronic Theses and Dissertations

Digestibilities of rations containing 10% (dry matter basis) oil-type, rolled sunflower seeds treated with three levels- of calcium hydroxide were compared using .a randomized complete block design. Forty-eight Holstein steers were fed one of six completely mixed rations consisting of (DM basis) 44% corn silage, 10% chopped alfalfa hay, and 46% concentrate mix. Concentrate mixes contained: CSM = control; SCS =control+ 22% sunflower seeds; SCL = SCS + 3.5% additional limestone; SCH2, SCH4, and SCH8 = SCS + 2, 4, and 8% calcium hydroxide (% sunflower seed DM), respectively. Total rations were isonitrogeneous at approximately 16% crude protein and isocaloric …

Lentinus Edodes Colonization Of Oak Is Enhanced By Log Treatment With Methyl Bromide, Elmer L. Schmidt

Journal of the Minnesota Academy of Science

Two or more years of outdoor incubation of inoculated oak logs are required before shiitake (Lentinus edodes [Berk.] Sing.) mushrooms appear; methods to accelerate the rate of oak sapwood colonization should reduce this incubation period. Observed increased mold growth of oak sapwood fumigated with methyl bromide (MB) prompted an experiment to determine whether such treatment would enhance oak colonization by L. edodes. Red and bur oak bolts ( 1 m long) were inoculated three weeks after felling. Half of the bolts were fumigated with MB ( 100 g per m3 of space beneath a plastic tarp) for three days, then …