Digital Commons Network^™

Contribution Of Rare Chromosome 22q112 Copy Number Variants To Non-Syndromic Bicuspid Aortic Valve, Helene Digregorio, Sara Mansoorshahi, Steven G Carlisle, Catherina Tovar Pensa, Abi Watts, Courtney Mcneely, Anna Sabate-Rotes, Anji Yetman, Hector I Michelena, Julie F A De Backer, Laura Muiño Mosquera, Malenka M Bissell, Maria Grazia Andreassi, Ilenia Foffa, Dawn S Hui, Anthony Caffarelli, Yuli Y Kim, Rodolfo Citro, Margot De Marco, Justin T Tretter, Kim L Mcbride, Simon C Body, Dianna M Milewicz, Siddharth K Prakash, Ebav Investigators

Faculty, Staff and Student Publications

Background: Bicuspid aortic valve (BAV) is the most common congenital heart defect in adults, often leading to complications such as thoracic aortic aneurysms and aortic stenosis. While BAV is frequently associated with 22q11.2 deletion syndrome (22q11.2DS), the contribution of rare copy number variants (CNVs) in this region to non-syndromic BAV is less clear. This study is aimed to assess the role of rare 22q11.2 CNVs in patients with early-onset BAV (EBAV) and to determine whether these variants are linked to an increased risk of complications.

Methods: Whole genome microarray genotyping was conducted on 272 patients with BAV with early onset …

Mapping Of Qtls Associated With Frost Tolerance In Olive (Olea Europaea L.), Öznur Çeti̇n, Nurengi̇n Mete, Handan Ölmez, Hati̇ce Gülen

Turkish Journal of Agriculture and Forestry

The olive (Olea europaea L.) is widely distributed due to its high adaptability to different environmental conditions. However,in recent years, sudden extreme temperature decreases and frost damage have caused significant yield and quality losses in some regions.This situation has led to the development of varieties with high frost tolerance, suitable for regions where cold damage may occur.It takes many years to develop varieties using classical breeding methods in olives. Therefore, early selection should be made usingbiotechnological methods. Markers about any trait provide a great advantage, especially in plant species with a very long juvenile sterilityperiod, as they allow for a …

The Role Of Brassinosteroids In Plant Physiological And Molecular Responses To Counter Salt Stress And Ensure Food Security: A Review And Future Perspectives, Muhammad Umair Hassan, Huang Guoqin, Muhammad Nawaz, Adnan Noor Shah, Tahir Abbas Khan, Muhammad Inzamam Ul Haq, Mehmood Ali Noor, Zhou Ping, Liu Qin, Yasser S. Mostafa, Saad A. Alamri, Melekber Sülüşoğlu Durul, Mehmet Ramazan Bozhüyük, Mohamed Hashem

Turkish Journal of Agriculture and Forestry

Excessive salinity poses serious threats to crop productivity and global food security. Salt stress reduces crop growth and final productivity by inducing ionic, osmotic, and oxidative damage. Applying plant growth hormones is thought to be an efficient method of reducing the negative effects of salinity. Brassinosteroids (BRs) are plant hormones that have demonstrated appreciable effects against different abiotic stresses. BRs can mitigate the deleterious impacts of salt stress by improving water and nutrient uptake, membrane stability, antioxidant activities, and osmolyte synthesis while maintaining the hormonal balance. Furthermore, BRs can also improve the metabolic and molecular responses of plants to help …

Chemical Composition And Larvicidal Toxicity Of Ricinus Communis L. (Euphorbiaceae) Extracts Against Larvae Of Thaumetopoea Pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae), Memi̇ş Kesdek, Şaban Kordali, Selçuk Küçükaydin

Turkish Journal of Agriculture and Forestry

In this study, the toxicities of extracts obtained from the seeds and leaves of castor bean (Ricinus communis L.) (Euphorbiaceae) plant against five larval stages of the pine processionary moth (PPM) (Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae)) were investigated under laboratory conditions. Petroleum ether, acetone, and methanol extracts were prepared at various concentrations (0.25, 0.5, and 1 mg/mL) and sprayed on each larval stage. Each test was performed with three replications and two repetitions. Sterile water + ethanol was used as a negative control and the commercial chemical Kormilin 25 WP (25% diflubenzuron) was used as a positive …

Identification And Functional Characterization Of The Icse1 Gene From Ilex Cornuta, Xiaoxi Zhang, Li Zhu, Weiwei Zhang, Feng Xu, Jiabao Ye, Yongling Liao

Turkish Journal of Agriculture and Forestry

Ilex cornuta (I. cornuta) is a traditional Chinese medicine that is rich in various secondary metabolites with pharmacological activities, among which triterpenoid saponins are one of the main active components. Squalene epoxidase (SE) is a key regulatory enzyme in the biosynthesis of triterpenoids. In this study, we isolated a squalene epoxidase (IcSE1) from I. cornuta and analyzed its molecular characteristics and functions. The results indicated that the total length of the IcSE1 sequence was 1942 bp, which includes a 1638 bp open reading frame (ORF) that encodes 545 amino acids. The theoretical isoelectric point (pI) and molecular weight of the …

A Genome-Wide Association Study To Detect Candidate Genes Controlling Iron And Manganese Accumulation In Walnut (Juglans Regia L.), Özge Doğanay, Erdal Orman, Bülent Yağmur, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Agriculture and Forestry

The walnut is one of the important low-glycemic sources of vegetable protein that benefit human health. Iron (Fe) and Manganese (Mn) are two of the walnut’s important nutritional components. This study aimed to identify single-nucleotide polymorphism (SNP) markers associated with the concentrations of Mn and Fe in walnut kernels and the genetic variation in these concentrations. The population was evaluated for two different years using 154 walnut genotypes and genotyping was performed with 16,473 SNP markers. A genome-wide association study was performed on 154 genotypes. A total of five and 15 SNPs were identified in different walnut genotypes, which were …

Phytochemical Analysis And In Vitro And In Vivo Pharmacological Activities Of Cordia Myxa Extracts, Tatheer Fatima, Mirza Imran Shahzad, Adnan Noor Shah, Zhang Yi, Bushra Gillani, Iqra Hussain, Adina Gillani, Zeli̇ha Çi̇ftçi̇, Marcello Locatelli, Ashraf Atef Hatamleh, Munirah Abdullah Al-Dosary

Turkish Journal of Agriculture and Forestry

Cordia myxa is found in tropical regions of Pakistan, where it is commonly known as lasura. This study aimed to assess the preliminary results of phytochemical analysis and the pharmacological activities of C. myxa. After pulverizing the fresh plant, extracts were prepared using different solvents, including aqueous, methanol, ethanol, dichloromethane, ethyl acetate, and n-hexane extracts. These extracts were examined for total phenolic contents using standard biochemical tests and high performance liquid chromatography with a photodiode array. In further evaluations, antioxidant, antibacterial, antiviral, hen’s egg test on chorioallantoic membrane (HETCAM) assay, antipyretic, antiinflammatory, and antidiabetic activities were monitored. Using ferric reducing …

Bioactive Content And Antioxidant Capacity Of Some Plants And Fruits Grown In Türkiye, Gülay Özkan, Fatma Betül Sakarya, Bayram Yurt, Elwira Sieniawska, Yakup Yapar, Esra Çapanoğlu Güven

Turkish Journal of Agriculture and Forestry

Recently, some edible plants and fruits have undergone extensive research to identify potential health effects arising from their valuable nutritional and bioactive properties. The present study aims to investigate the phenolic compounds of nine edible plants and fruits throughout the gastrointestinal digestion model by comparing their total content and antioxidant activity. Of the species studied, Eremurus spectabilis and Gundelia tournefortii had the lowest total phenolic content, whereas Prunus armeniaca showed the highest value of 1026 ± 164 mg GAE/100 g. Except for Prunus armeniaca , the total phenolic contents of all samples were increased following gastric digestion, while the total …

Restore The Shore: A Guide To Lakeshore Revegetation In The Great Basin, Jes V. Braun, Rae Robinson, Annie L. Henry, Karin M. Kettenring

All Current Publications

Lakeshore vegetation supports many valuable aspects of lake ecosystems: foraging areas for birds, rearing grounds for fish, nutrient cycling, carbon storage, and aesthetic, cultural, and spiritual values. Yet, the vegetation of many lakeshores has been lost or heavily impacted by humans. What can be done to restore lakeshore vegetation to ensure that lakes remain healthy for future generations? In this guide, we summarize best practices for lakeshore revegetation (i.e., the process of actively reintroducing vegetation, usually native species, to a restoration site through seeding and planting) in the Great Basin.

Exploring Genetic Diversity: The Inheritance Of Protein And Mineral Contents In Dwarf Common Beans, Ercan Ceyhan, Ayşegül Korkmaz, Amjad Ali̇, Serdar Karadaş, Mustafa Harmankaya, Duran Şi̇mşek, Tolga Karaköy

Turkish Journal of Agriculture and Forestry

Beans are a vital plant-based protein, containing essential minerals that are necessary for human health. Their protein and mineral compositions vary due to factors including cultivar or variety, growth conditions, and processing methods. Global deficiencies in protein and minerals necessitate fortification, supplementation, and dietary diversification. The present study focuses on common bean (Phaseolus vulgaris) cultivars, aiming to understand their genetic variation and inheritance of protein and mineral contents. Five dwarf common bean cultivars (Lida, Beyzade, Ribera, Albeni, and Garrafol) were used in a full-diallel hybridization method and the experiment was conducted in Konya, Türkiye. The diallel analysis assessed both general …

Enhancing Salt Tolerance And Yield Potential In Cotton: Insights From Physiological Responses, Genetic Variability, And Heterosis, Muhammad Mubashar Zafar, Abdul Razzaq, Zunaira Anwar, Aqsa Ijaz, Muhammad Zahid, Muhammad Shahid Iqbal, Ghulam Farid, Mahmoud F. Seleiman, Rana Qammar Uz Zaman, Abdur Rauf, Xuefei Jiang

Turkish Journal of Agriculture and Forestry

Soil salinity is a growing global concern, necessitating the development of salt-tolerant cotton cultivars to enhance the utilization of salt-affected soils and boost crop productivity. Salt tolerance is regulated by a complex interplay of genetic and physiological factors involving multiple genes. To investigate the genetic basis of salt stress tolerance, eight parental genotypes (four lines and four testers) and their 16 hybrid combinations were evaluated under normal and salt stress conditions (15 dS m⁻¹). Data were collected on various traits including the plant height (PH), number of bolls per plant (NBP), boll weight (BW), lint percentage (LP), seed cotton yield …

Nutritional And Microbiological Properties Of Cheese With Dried Elderberry And Red Grape Pomace, Andreea Dodan, Romina Alina Marc, Crina Carmen Mureşan, Carmen Rodica Pop, Delia Michiu, Miuța Rafila Filip, Roxana Nicoleta Rațu, Florina Stoica, Ioana Cristina Crivei, Alina Narcisa Postolache, Melinda Kovacs, Rodica Maria Sima, Mircea Valentin Muntean

Turkish Journal of Agriculture and Forestry

This study aimed to explore the use of grape byproducts such as red grape pomace and elderberry, in the form of bioactive powders to obtain three-month old cheese assortments. Grape pomace powder (GPP) and elderberry fruit powder (EFP) contain antioxidant components such as phenols, anthocyanins and flavonoids, and compounds characterized by high bioactivity. This study investigated not only the potential of incorporating different percentages (1%, 2%, and 3%) of GPP and EFP in cheese, to increase its nutritional value, but also the changes that occur during the 2 months of ripening. The results indicated that the incorporation of GPP and …

Transcriptome And Metabolome Analysis Of Low-Temperature Storage On Fruit Quality Deterioration Of Mangoes, Hanqing Cong, Ting Huo, Xinran Huang, Yeyuan Chen, Yibo Bai, Haiyan Luo, Xiaona Fu, Rongxiang Wang, Mahmoud F. Seleiman, Tanweer Ahmad, Pingyin Guan, Mohsin Bashir, Muhammad Asif, Fei Qiao

Turkish Journal of Agriculture and Forestry

Mango, a typical respiratory climacteric fruit, is susceptible to a short shelf life and quality deterioration during postharvest ripening. Although low temperature can prolong the storage time significantly, its effects on fruit quality have yet to be elucidated. Through a combination of transcriptome and metabolome analyses, this study aimed to elucidate the effects of low-temperature storage on the biosynthesis of volatile organic compounds (VOCs), flavonoids, sucrose, and the degradation of organic acids in mango. Carbohydrate-related metabolism maintained high activity when stored at 13 °C by upregulating the expression of certain gene members in the neutral invertase (NI), sucrose phosphate synthase …

Understanding The Role Of Thermal Stress In Modulating Growth, Yield, And Quality Attributes Of Tomato At Two Locations, Muhammad Uzair, Rabia Kalsoom, Kotb A. Attia, Irfan Ali, Tanveer Hussain, Asmaa M. Abushady, Haider Ali, Sajid Fiaz, Saima Jameel, Muhammad Usman Hameed, Rashid Iqbal, Muhammad Ramzan Khan

Turkish Journal of Agriculture and Forestry

Tomato is a well-recognized and extensively cultivated crop on a global scale. However, the development of tomato varieties is hindered by adverse environments, limited genetic diversity, and low rates of seed setting. In this study, we evaluated 21 genotypes at two different locations in Pakistan. We analyzed morphological traits including vine length, days to 50% flowering, days to fruit setting initiation, number of clusters per plant (NCP), and number of flowers per cluster (NFC); yield-related traits such as fruit setting percentage per cluster, fruit setting percentage per plant, number of marketable fruits per plant (NMFP), marketable fruit yield per plant …

Agronomic Performance Of Fenugreek Genotypes Grown Under Various Sowing And Nitrogen Rates In A Highland Environment, Furkan Çoban, Hakan Özer

Turkish Journal of Agriculture and Forestry

Fenugreek is an important medicinal plant used in food, medicine, and other industries. In recent years, there has been increased interest in the fenugreek plant, with increased support given for fenugreek in Türkiye. However, there is a need for sound recommendations on agronomic practices such as variety selection, plant density, and fertilization due to local differences in the climate and soil structure. This research was conducted to determine the yield and some agricultural characteristics of two fenugreek genotypes (Turkish and Iranian) grown under different nitrogen (N) (0, 20, 40, 60, and 80 kg ha–1) and sowing (20, 40, and 60 …

Complete Genome Sequence Of A Penicillin-Resistant Fusobacterium Necrophorum Subsp Funduliforme Isolate From A Tonsillitis Patient, Bibek G C, Anders Jensen, Chenggang Wu

Faculty, Staff and Student Publications

We report the complete genome sequence of a penicillin-resistant Fusobacterium necrophorum subsp. funduliforme isolate, AJ79, from a tonsillitis patient. The AJ79 genome consists of a chromosome (2,440,359 bp) and plasmid (9,887 bp), providing insights into the genetic basis of penicillin resistance in F. necrophorum and its implications for treating tonsillitis.

Nprl2 Gene Therapy Induces Effective Antitumor Immunity In Kras/Stk11 Mutant Anti-Pd1 Resistant Metastatic Non-Small Cell Lung Cancer (Nsclc) In A Humanized Mouse Model, Ismail M Meraz, Mourad Majidi, Renduo Song, Feng Meng, Lihui Gao, Qi Wang, Jing Wang, Elizabeth J Shpall, Jack A Roth

Faculty, Staff and Student Publications

Expression of NPRL2/TUSC4, a tumor-suppressor gene, is reduced in many cancers including NSCLC. Restoration of NPRL2 induces DNA damage, apoptosis, and cell-cycle arrest. We investigated NPRL2 antitumor immune responses in aPD1R/KRAS/STK11^mt NSCLC in humanized-mice. Humanized-mice were generated by transplanting fresh human cord blood-derived CD34 stem cells into sub-lethally irradiated NSG mice. Lung-metastases were developed from KRAS/STK11^mt/aPD1R A549 cells and treated with NPRL2 w/wo pembrolizumab. NPRL2-treatment reduced lung metastases significantly, whereas pembrolizumab was ineffective. Antitumor effect was greater in humanized than non-humanized-mice. NPRL2 + pembrolizumab was not synergistic in KRAS/STK11^mt/aPD1R tumors but was …

Engineered Immunomodulatory Extracellular Vesicles From Epithelial Cells With The Capacity For Stimulation Of Innate And Adaptive Immunity In Cancer And Autoimmunity, Xin Luo, Fernanda G Kugeratski, Dara P Dowlatshahi, Hikaru Sugimoto, Kent A Arian, Yibo Fan, Li Huang, Danielle Wills, Sergio Lilla, Kelly Hodge, Sara R Zanivan, Valerie S Lebleu, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) are generated by all cells. Systemic administration of allogenic EVs derived from epithelial and mesenchymal cells have been shown to be safe, despite carrying an array of functional molecules, including thousands of proteins. To address whether epithelial cells derived EVs can be modified to acquire the capacity to induce immune response, we engineered 293T EVs to harbor the immunomodulatory molecules CD80, OX40L and PD-L1. We demonstrated abundant levels of these proteins on the engineered cells and EVs. Functionally, the engineered EVs efficiently elicited positive and negative co-stimulation of human and murine T cells. In the setting of …

Trpv4 Dominates High Shear-Induced Initial Traction Response And Long-Term Relaxation Over Piezo1, Mohanish Chandurkar, Manli Yang, Majid Rostami, Sangyoon J. Han

Michigan Tech Publications

Modulation of endothelial traction is critical for the responses of endothelial cells to fluid shear stress (FSS), which has profound implications for vascular health and atherosclerosis. Previously, we demonstrated that under high FSS, endothelial cells rapidly increase traction forces, followed by relaxation, with traction aligning in the flow direction. In contrast, low shear preconditioning induces a modest short-term increase in traction (min), followed by a secondary long-term (>14 hr) rise, with traction/cells aligning perpendicular to the flow. The upstream mechanosensors driving these responses, however, remain unknown. Here, we sought the roles of Piezo1 and TRPV4 ion channels in shear-induced …

Exploring The Therapeutic Potential Of Resveratrol In Substance Use Disorders: A Systematic Review Of Its Effects On Kcc2 Expression, Lauren R. Nelson, Ashlyn Hughes, Jory Winward, Natalie Phanco, Andrew Payne Phd

Annual Research Symposium

No abstract provided.

Use Of Synthetic Water-Soluble Kcc2 To Modulate Chloride Homeostasis In Cultured Dopamine-Neuron-Like Cells, Mason Terry, Andrew Payne, Dario Mizrachi

Annual Research Symposium

No abstract provided.

Characterization Of 1,8-Cineole (Eucalyptol) From Myrtle And Its Potential Antibacterial And Antioxidant Activities*, Humera Khan

Karbala International Journal of Modern Science

1,8-Cineole is a naturally occurring chemical molecule predominantly found in fragrant plants, particularly Myrtle. Its aroma is distinctive and has been the subject of numerous investigations due to its various biological actions. This study examines the characterization of 1,8-Cineole derived from Myrtle and investigates its antibacterial and antioxidant properties. This study seeks to compare 1,8-Cineole with antibiotics like Amoxicillin and Tetracycline, and moreover, to investigate its antioxidant capabilities against diverse bacterial strains (both Gram-positive and Gram-negative). 1,8-Cineole exhibits the most effective antibacterial properties, demonstrating an inhibition zone of 12.00 mm against Staphylococcus aureus and 10.00 mm against Pseudomonas aeruginosa. …

Structural, Molecular And Developmental Evidence For Cell-Type Diversity In Cnidarian Mechanosensory Neurons, Julia Baranyk, Kristen Malir, Miguel A.P. Silva, Sakura Rieck, Gracie Scheve, Nagayasu Nakanishi

Biological Sciences Faculty Publications and Presentations

Deploying a conserved mechanosensory neuron known as the concentric hair cell, cnidarians have evolved diverse mechanoreceptors from hydroid filiform tentacles to jellyfish statocysts. However, it is unknown whether cnidarian mechanoreceptor evolution has relied solely on repurposing a single ancestral mechanosensory neuron type. Here we report evidence for cell-type diversity of mechanosensory neurons in sea-anemone cnidarian Nematostella vectensis. Uncovered in the ectoderm of feeding tentacles are conventional type I hair cells and previously unrecognized type II hair cells differing in the structure of apical sensory apparatus and synapses. Moreover, we identify TRP channel-encoding gene polycystin-1 as a type-II-hair-cell-specific essential mediator of …

A Deep Learning-Based System For Automatic Detection Of Emesis With High Accuracy In Suncus Murinus, Zengbing Lu, Yimeng Qiao, Xiaofei Huang, Dexuan Cui, Julia Y H Liu, Man Piu Ngan, Luping Liu, Zhixin Huang, Zi-Tong Li, Lingqing Yang, Aleena Khalid, Yingyi Deng, Sze Wa Chan, Longlong Tu, John A Rudd

Children’s Nutrition Research Center Staff Publications

Quantifying emesis in Suncus murinus (S. murinus) has traditionally relied on direct observation or reviewing recorded behaviour, which are laborious, time-consuming processes that are susceptible to operator error. With rapid advancements in deep learning, automated animal behaviour quantification tools with high accuracy have emerged. In this study, we pioneere the use of both three-dimensional convolutional neural networks and self-attention mechanisms to develop the Automatic Emesis Detection (AED) tool for the quantification of emesis in S. murinus, achieving an overall accuracy of 98.92%. Specifically, we use motion-induced emesis videos as training datasets, with validation results demonstrating an accuracy of 99.42% for …

Axl Signaling In Cancer: From Molecular Insights To Targeted Therapies, Monika Yadav, Akansha Sharma, Ketki Patne, Saba Tabasum, Jyoti Suryavanshi, Laxminarayan Rawat, Marc Machaalani, Marc Eid, Rana Singh, Toni Choueiri, Soumitro Pal, Akash Sabarwal

Department of Microbiology and Immunology Faculty Papers

AXL, a member of the TAM receptor family, has emerged as a potential target for advanced-stage human malignancies. It is frequently overexpressed in different cancers and plays a significant role in various tumor-promoting pathways, including cancer cell proliferation, invasion, metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, stemness, DNA damage response, acquired therapeutic resistance, immunosuppression, and inflammatory responses. Beyond oncology, AXL also facilitates viral infections, including SARS-CoV-2 and Zika highlighting its importance in both cancer and virology. In preclinical models, small-molecule kinase inhibitors targeting AXL have shown promising anti-tumorigenic potential. This review primarily focuses on the induction, regulation and biological functions of AXL …

Classification Of Non-Tcga Cancer Samples To Tcga Molecular Subtypes Using Compact Feature Sets, Kyle Ellrott, Christopher K Wong, Christina Yau, Mauro A A Castro, Jordan A Lee, Brian J Karlberg, Jasleen K Grewal, Vincenzo Lagani, Bahar Tercan, Verena Friedl, Toshinori Hinoue, Vladislav Uzunangelov, Lindsay Westlake, Xavier Loinaz, Ina Felau, Peggy I Wang, Anab Kemal, Samantha J Caesar-Johnson, Ilya Shmulevich, Alexander J Lazar, Ioannis Tsamardinos, Katherine A Hoadley, Cancer Genome Atlas Analysis Network, A Gordon Robertson, Theo A Knijnenburg, Christopher C Benz, Joshua M Stuart, Jean C Zenklusen, Andrew D Cherniack, Peter W Laird

Faculty, Staff and Student Publications

Molecular subtypes, such as defined by The Cancer Genome Atlas (TCGA), delineate a cancer's underlying biology, bringing hope to inform a patient's prognosis and treatment plan. However, most approaches used in the discovery of subtypes are not suitable for assigning subtype labels to new cancer specimens from other studies or clinical trials. Here, we address this barrier by applying five different machine learning approaches to multi-omic data from 8,791 TCGA tumor samples comprising 106 subtypes from 26 different cancer cohorts to build models based upon small numbers of features that can classify new samples into previously defined TCGA molecular subtypes-a …

Sex And Outcomes Of Patients With Microsatellite Instability-High And Braf V600e Mutated Metastatic Colorectal Cancer Receiving Immune Checkpoint Inhibitors, Vincenzo Nasca, Joseph Zhao, Javier Ros, Sara Lonardi, Koen Zwart, Romain Cohen, Marwan Fakih, Priya Jayachandran, Jeanine M L Roodhart, Jeroen Derksen, Rossana Intini, Francesca Bergamo, Giacomo Mazzoli, Filippo Ghelardi, Marta Ligero, Jitendra Jonnagaddala, Nicholas Hawkins, Robyn L Ward, Durgesh Wankhede, Hermann Brenner, Michael Hoffmeister, Marco Vitellaro, Lisa Salvatore, Claire Gallois, Pierre Laurent-Puig, Chiara Cremolini, Michael J Overman, Julien Taieb, David Tougeron, Thierry Andre, Jakob Nikolas Kather, Raghav Sundar, Javier Carmona, Elena Elez, Miriam Koopman, Filippo Pietrantonio

Faculty, Staff and Student Publications

Background: Immune checkpoint inhibitors (ICIs) are the gold standard therapy in patients with deficient mismatch repair (dMMR)/microsatellite instability-high (MSI-H) metastatic colorectal cancer (mCRC). A significant proportion of patients show resistance, making the identification of determinants of response crucial. Growing evidence supports the role of sex in determining susceptibility to anticancer therapies, but data is lacking for patients with MSI-H CRC.

Methods: In this real-world cohort comprising 624 patients with MSI-H mCRC receiving ICIs, we investigated the impact of sex on patients' outcomes, overall and according to RAS-BRAF mutational status or type of treatment (anti-PD-(L)1 with or without anti-CTLA-4 agents). We …

Tigit Inhibitor M6223 As Monotherapy Or In Combination With Bintrafusp Alfa In Patients With Advanced Solid Tumors: A First-In-Human, Phase 1, Dose-Escalation Trial, Aung Naing, Meredith Mckean, Anthony Tolcher, Anja Victor, Ping Hu, Wei Gao, Marco A F Nogueira Filho, Thomas Kitzing, Stephan Gleicher, Daniel Holland, Emilia Richter, Keyvan Tadjalli-Mehr, Lillian L Siu

Faculty, Staff and Student Publications

Background: M6223 is an intravenous (IV), Fc-competent, fully human, antagonistic, anti-T cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) antibody. Bintrafusp alfa (BA) is a bifunctional fusion protein that simultaneously blocks nonredundant immunosuppressive TGF-β and PD-(L)1 pathways.

Methods: This first-in-human, dose-escalation study in patients with advanced solid tumors (N=58; aged ≥18 years, ECOG PS≤1) evaluated M6223 alone (Part 1A, n=40; M6223 10-2400 mg every 2 weeks, n=32; M6223 2400 mg every 3 weeks, n=8) or with BA (Part 1B, n=18; M6223 300-1600 mg with BA 1200 mg; both every 2 weeks, intravenous). Primary objectives were safety, tolerability, …

Atsubp-2.0: An Integrated Web Server For The Annotation Of Arabidopsis Proteome Subcellular Localization Using Deep Learning, Naveen Duhan, Rakesh Kaundal

Plants, Soils and Climate Student Research

The organization of subcellular components in a cell is critical for its function and studying cellular processes, protein–protein interactions, identifying potential drug targets, network analysis, and other systems biology mechanisms. Determining protein localization experimentally is time-consuming and expensive. Due to the need for meticulous experimentation, validation, and data analysis, computational methods provide a quick and accurate alternative. Arabidopsis thaliana, a beneficial model organism in plant biology, facilitates experimentation and applies to other plants. Predicting its proteins' subcellular localization can improve our understanding of cellular processes and have applications in crop improvement and biotechnology. We propose AtSubP-2.0, an extension of …

Life History, Reproductive, And Demographic Parameters For Bottlenose Dolphins (Tursiops Truncatus) In Sarasota Bay, Florida, Randall S. Wells, Aleta A. Hohn, Michael D. Scott, Jay C. Sweeney, Forrest I. Townsend, Jason B. Allen, Aaron A. Barleycorn, Katherine A. Mchugh, Kim Bassos-Hull, Gretchen N. Lovewell, Deborah A. Duffield, Cynthia R. Smith, A. Blair Irvine

Biology Faculty Publications and Presentations

Studies of the resident community of bottlenose dolphins (Tursiops truncatus) of Sarasota Bay, Florida, have been conducted for more than 50 years. Detailed histories have been collected for resident individuals through integrated observations, systematic photographic identification surveys, tagging and tracking, catch-and-release health assessments, remote biopsy sampling, and stranding response. This has produced a unique dataset documenting life history milestones and vital rates of a small cetacean. Analyses of data from 482 resident Sarasota Bay dolphins have revealed estimated maximum life spans of 67 years for females and 52 years for males. For females, predicted age at sexual maturation …