Digital Commons Network^™

Accumulation Of Cd38 In Hybrid Epithelial/Mesenchymal Cells Promotes Immune Remodeling And Metastasis In Breast Cancer, Tanvi H Visal, Recep Bayraktar, Petra Den Hollander, Michael A Attathikhun, Tieling Zhou, Jing Wang, Li Shen, Corina-Elena Minciuna, Meng Chen, Elizve Barrientos-Toro, Harsh Batra, Maria Gabriela Raso, Fei Yang, Edwin R Parra, Aysegul A Sahin, George A Calin, Sendurai A Mani

Faculty, Staff and Student Publications

Triple-negative breast cancer (TNBC) is a highly metastatic subtype of breast cancer. The epithelial-to-mesenchymal transition is a nonbinary process in the metastatic cascade that generates tumor cells with both epithelial and mesenchymal traits known as hybrid EM cells. Recent studies have elucidated the enhanced metastatic potential of cancers featuring the hybrid EM phenotype, highlighting the need to uncover molecular drivers and targetable vulnerabilities of the hybrid EM state. Here, we discovered that hybrid EM breast tumors are enriched in CD38, an immunosuppressive molecule associated with worse clinical outcomes in liquid malignancies. Altering CD38 expression in tumor cell impacted migratory, invasive, …

Antibody-Drug Conjugates Targeting The Egfr Ligand Epiregulin Elicit Robust Antitumor Activity In Colorectal Cancer, Joan Jacob, Yasuaki Anami, Peyton C High, Zhengdong Liang, Shraddha Subramanian, Sukhen C Ghosh, Solmaz Aghaamiri, Cara Guernsey-Biddle, Ha Tran, Julie Rowe, Ali Azhdarinia, Kyoji Tsuchikama, Kendra S Carmon

Faculty, Staff and Student Publications

As colorectal cancer remains a leading cause of cancer-related death, identifying therapeutic targets and approaches is essential to improve patient outcomes. The EGFR ligand epiregulin (EREG) is highly expressed in RAS wild-type (WT) and mutant colorectal cancer, with minimal expression in normal tissues, making it an attractive target for antibody-drug conjugate (ADC) development. In this study, we produced and purified an EREG mAb, H231, which had high specificity and affinity for human and mouse EREG. H231 also internalized to lysosomes, which is important for ADC payload release. ImmunoPET and ex vivo biodistribution studies showed significant tumor uptake of zirconium-89-labeled H231, …

Epigenetic Landscapes Of Aging In Breast Cancer Survivors: Unraveling The Impact Of Therapeutic Interventions-A Scoping Review, Nikita Nikita, Zhengyang Sun, Swapnil Sharma, Amy L Shaver, Victoria Seewaldt, Grace Lu-Yao

Department of Medical Oncology Faculty Papers

Breast cancer therapies have dramatically improved survival rates, but their long-term effects, especially on aging survivors, need careful consideration. This review delves into how breast cancer treatments and aging intersect, focusing on the epigenetic changes triggered by chemotherapy, radiation, hormonal treatments, and targeted therapies. Treatments can speed up biological aging by altering DNA methylation, histone modifications, and chromatin remodeling, affecting gene expression without changing the DNA sequence itself. The review explains the double-edged sword effect of therapy-induced epigenetic modifications, which help fight cancer but also accelerate aging. Chemotherapy and targeted therapies, in particular, impact DNA methylation and histone modifications, promoting …

Impact Of Fresh-Cut Onion Types And Flavonoid Profiles On The Survival Kinetics Of Foodborne Pathogen During Refrigerated Storage, Veerachandra Yemmireddy, Matthew Mancias

School of Earth, Environmental, & Marine Sciences Faculty Publications

This study investigated the effect of onion flavonoid profiles on the growth, survival, and/or death kinetics of foodborne pathogens in fresh-cut onions at 4 °C. Fresh-cut white, yellow, red, and sweet onions were inoculated with separate four-strain cocktail(s) of nalidixic acid-adapted Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes achieving a 4 to 5 log CFU g−1. The samples were stored in clam shell containers at 4 °C and 70 ± 2 % relative humidity for 14 days. Additionally, flavonoid profiles were determined on uninoculated control samples stored under similar conditions using standard chromatography techniques, with quercetin derivatives identified as …

Cargo Hitchhiking Autophagy - A Hybrid Autophagy Pathway Utilized In Yeast, Katrina F Cooper

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Macroautophagy is a catabolic process that maintains cellular homeostasis by recycling intracellular material through the use of double-membrane vesicles called autophagosomes. In turn, autophagosomes fuse with vacuoles (in yeast and plants) or lysosomes (in metazoans), where resident hydrolases degrade the cargo. Given the conservation of autophagy,

Using Stable Isotope Analysis To Investigate The Trophic Ecology Of Aquatic Insects, Shelby Ann Medlock

Master's Theses

Despite their abundance on the landscape, ponds and associated macroinvertebrate assemblages have largely been underrepresented in scientific studies, which usually focus on lakes or streams. Similar to other waterbodies, ponds can harbor high levels of invertebrate biodiversity, which is important as aquatic insects are experiencing declines due to habitat destruction, pollution, climate change, and invasive species introductions. To evaluate how these threats impact pond insects and the ecosystem services they may provide, a thorough understanding of their ecology is essential. However, significant knowledge gaps remain, especially regarding trophic levels and species-specific diets. To address these knowledge gaps, I used carbon …

Associations Among Shopping Methods, Diet Adherence, And Nutrition Knowledge Of Employed Primary Grocery Shoppers In Households With Children, Baylee Schneider

Master's Theses

Obesity is a prominent risk factor for chronic disease, which is the leading cause of death in the United States. Obesity and chronic disease rates continue to rise, requiring a need for prevention and intervention efforts. The future role of dietetics is to provide treatment and prevention through holistic approaches, by understanding individuals’ habits that contribute to the onset of disease, specifically their food choices, grocery shopping methods, and influences that waver these selections. The purpose of this study is to evaluate the relationship between grocery shopping methods (online or in-store), Mediterranean diet adherence, nutrition knowledge, and food purchase patterns …

Comparison Of Caregiver-Reported Dietary Intake Methods In Zellweger Spectrum Disorder, Mousumi Bose, Nancy L. Von Thun, Adrian L. Kerrihard, Melisa L. Lopez, Chelsea I. Donlon, Alyssa K. Smolen, Nicole P. Fontes

Department of Nutrition and Food Studies Scholarship and Creative Works

Background/Objectives: Zellweger spectrum disorder (ZSD), a rare genetic disease characterized by defects in peroxisome biogenesis, results in dysfunction of all organ systems, including feeding difficulties, gastrointestinal bleeding, and reduced overall growth. Despite this nutritional impact, no published studies have assessed dietary intake in ZSD. The purpose of this study was to determine nutrient intake in individuals with ZSD or a related peroxisomal disorder using two methods of dietary assessment as provided by family caregivers. Methods: Family caregivers participated in multiple 24 h dietary recall interviews and completed 3-day food records for their child with ZSD or a related single-enzyme peroxisomal …

An Exploratory Case Study Of A Community Food Pantry In Northwest Louisiana, Abigail Clair Henington

Master's Theses

Many individuals are struggling with food insecurity in Louisiana; according to the Louisiana Food Bank Association's website, Feeding Louisiana’s 2023 impact report, an estimated 683,100 people in Louisiana experience food insecurity. In northwest Louisiana, 30% of the population lives below the poverty line, with an average income of $34,485 per household. Common Ground is a nonprofit community outreach program in Northwest Louisiana, specifically Shreveport. The program has been in operation for over 20 years and strives to provide a place free of judgment and a place of common ground for those in the Cedar Grove community. Each week, they have …

Cryptosporidium Virus Integration Into The Host Cells, Alyssa Genaro

Biochemistry and Molecular Biology

Cryptosporidiosis is a disease that affects a large amount of people and having a high mortality rate for those that are immunocompromised such as the elderly and infants. The disease is linked to a parasite called Cryptosporidium parvum, which is mostly known to infect cattle and other mammals such as mice and humans. The parasite contains a double stranded RNA virus, the Cryspovirus (CSpV1) that has a non-enveloped icosahedral capsid, with a T=2* icosahedral symmetry. Little is known is known about the contribution of CSpV1 to the disease; In this study we show that infection of an immunocompromised mouse model …

Bright Ideas: How Leaf Cells Shape The Way Plants Capture Light, Nicola Trozzi, Janlo M. Robil

Biology Faculty Publications

No abstract provided.

Impact Of Polysaccharides On Advancing Plant-Based Meat Alternatives, Prashant Dahal, Clifford Hall, Srinivas Janaswamy

Dairy Science and Food Science Faculty Publications: 2022 to Present

Plant-based meat products are a valued choice for vegetarians and others. Their production has been perceived as an innovation promoting animal welfare and sustainability. However, there are challenges in simulating desirable appearance, texture, and flavor using only plant-based protein sources. Polysaccharide addition during the formulation and texturization of plant proteins can improve the physi- cochemical and sensory properties of texturized protein products. In this regard, a clear understanding of the fundamental properties of polysaccharides is essential for transforming plant proteins into fibers or nonfilamentous protein aggregates. Enhancing water-holding capacity, gelation, and binding functionality is crucial for overcoming structural and textural …

The Nebraska Bird Review (March 2025) 93(1), Whole Issue

Nebraska Bird Review

Winter Field Report, December 2024–February 2025, by W. Ross Silcock
2024–2025 Christmas Bird Counts, by Don Paseka
Annual Color Photo Section
Annual Treasurer’s Report for 2024, by Jan Johnson
Subscription and Organization Information

Annual Color Photo Section

Nebraska Bird Review

The following seven pages show examples of this year’s theme: birds feeding or carrying food.

Osprey with striped bass
Spotted Sandpiper with beetle
Scissor-tailed Flycatcher with insect
Male Pine Grosbeak with green ash seeds
Merlin eating sparrow
Whooping Crane carrying ear of corn
Yellow-billed Cuckoo with harvestman spider
Black-billed Magpie eating grain
Red-tailed Hawk with plains garter snake
Summer Tanager with hackberry emperor butterfly
Cattle Egret with bullfrog
Juvenile Red-shouldered Hawk eating garter snake
Red-breasted Merganser eating fish
Juvenile White Ibis eating unidentified item
Bohemian Waxwing eating crab apples
Rose-breasted Grosbeak eating sunflower seeds
Loggerhead Shrike eating dragonfly
Yellow-crowned Night …

2024–2025 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

For the second year in a row, all twenty CBCs [Christmas Bird Counts] were run during the 2024–2025 season. The weather was generally cooperative, although five circles reported fog and two others had rain. Branched Oak-Seward was postponed a week due to icy conditions on the originally scheduled day. The average high temperature for all circles on count day was 41 and the average low temperature was 22. Because December had remained relatively mild, there was a good deal of open water, which encouraged many species of water birds to remain. Consequently, most of the circles reported species totals higher …

Winter Field Report, December 2024–February 2025, W. Ross Silcock

Nebraska Bird Review

Since I started a few years ago to compare each season’s reports with the status of each species as presented in BONO (Species Accounts – Birds of Nebraska – Online), I had expected that “Routine reports” would apply to an increasing number of species as the BONO database firmed up, but this hasn’t happened. My comments from last winter continue to be valid this winter, now for the third winter in a row: As was the case for winter 2022–2023, the big general story was the large number of reports of tardy fall migrants and unexpectedly northerly occurrences in mid-winter… …

Alfred Russel Wallace Notes 35. Natural Selection By Evolution., Charles H. Smith

Faculty/Staff Personal Papers

Alfred Russel Wallace (1823?1913) is remembered, with Charles Darwin, as the co-originator of the concept of evolution by natural selection. But Wallace’s framing of the idea increasingly became one in which natural selection itself was regarded as a law of nature, not a theory per se.

A Preliminary Report Of The Carotenoid Assessment In Adults 50 Years Of Age And Older In Louisiana, Mary-Catherine Feazell

Master's Theses

Fruits and vegetables are at the core of a healthy, balanced diet. They assist in preventing the development of chronic diseases such as hypertension, diabetes, cardiovascular disease, and cancer (Fadnes, et al., 2022). Aging is a natural biological process followed by disease, cognitive impairment, and a natural decline of the body’s ability to perform at its best. The older adult population is currently the fastest-growing population globally (El-Shebiney et al., 2022). Vitamin A is crucial for several parts of the body to function correctly. Despite the positive effects of Vitamin A on the body, the recommended dietary intake is not …

Ecology Of Ixodes Scapularis Say: Experimental And Observational Studies With Social Perspectives On Ticks And Tick-Borne Diseases, Kaitlin M. Mcdonald

Dartmouth College Ph.D Dissertations

Tick-borne diseases are a growing concern globally. Understanding the factors that influence tick ecology and people’s understanding of that ecology is crucial for developing effective tick management and disease prevention strategies. In this work, I investigated fundamental questions about the ecology of a primary vector of medically important pathogens in the United States, the blacklegged tick Ixodes scapularis Say, and identified common misconceptions about ticks and tick-borne diseases on social media. First, I examined the effects of temperature and moisture stress on the movement of adult female I. scapularis in laboratory experiments. Using a Drosophila Activity Monitor, ticks were exposed …

Optimizing Biofungicide Use In Managing Winegrape Powdery Mildew On The Central Coast Of California, Edgar G. Godoy Monterroso

Master's Theses

Grapevine powdery mildew caused by the fungal pathogen Erysiphe necator is a persistent threat to wine grape production in California. While synthetic fungicides remain the primary management strategy, increasing concerns over pesticide resistance, environmental impact, and regulatory restrictions have driven interest in biofungicides as a sustainable alternative. However, their efficacy is inconsistent, requiring research into optimized application strategies, integration with synthetic fungicides, and their effects on disease suppression, grape yield, and fruit quality.

This two-year study (2023–2024) was conducted at two vineyard sites, Trestle Vineyard in the San Luis Obispo Coast AVA and a commercial vineyard in the Arroyo Grande …

Wright State University's Celebration Of Student Research, Scholarship & Creative Activities From Thursday, March 13, 2025, Wright State University

Celebration of Undergraduate & Graduate Research, Scholarship, and Creative Activities Abstract Books

The student abstract booklet is a compilation of abstracts from students' oral and poster presentations at Wright State University's Celebration of Student Research, Scholarship & Creative Activities on March 13, 2025.

Lagging Brain Gene Expression Patterns Of Drosophila Melanogaster Young Adult Males Confound Comparisons Between Sexes, Flannery Mclamb, Zuying Feng, Jeanne P. Vu, Lindsey Griffin, Miguel F. Vasquez, Goran Bozinovic

Center for Life in Extreme Environments Publications

Many species, including fruit flies (Drosophila melanogaster), are sexually dimorphic. Phenotypic variation in morphology, physiology, and behavior can affect development, reproduction, health, and aging. Therefore, designating sex as a variable and sex-blocking should be considered when designing experiments. The brain regulates phenotypes throughout the lifespan by balancing survival and reproduction, and sex-specific development at each life stage is likely. Changes in morphology and physiology are governed by differential gene expression, a quantifiable molecular marker for age- and sex-specific variations. We assessed the fruit fly brain transcriptome at three adult ages for gene expression signatures of sex, age, and sex-by-age: 6698 …

Antibiotic Resistome, Mobile Genetic Elements, And Virulence Profile Of Multidrug-Resistant Enterobacteriaceae Isolated From Healthy Philippine Native Chicken As Revealed By Analysis Of Whole Genome Sequence, Alvin P. Jimenez, Grignard James R. Buday, Rina B. Opulencia

The Philippine Agricultural Scientist

Gram-negative bacteria, particularly Enterobacteriaceae, acquire antibiotic resistance via horizontal transmission of resistance genes in poultry farms that give rise to multidrug-resistant (MDR) strains. The presence of MDR strains that are potentially pathogenic and zoonotic poses a concern to animal and human health. The aims of this study were to isolate MDR, potentially pathogenic Enterobacteriaceae from Philippine native chicken (PNC) and to analyze the genome of a representative MDR isolate to gain insights into the mechanisms, acquisition, and transmissibility of antibiotic resistance genes (ARGs). Cloacal swabs from PNC were used to isolate putative Salmonella that were then tested for antimicrobial …

Assessment Of Water Pollution Levels In Lake Batur, Indonesia And Their Effect On Water Weed Presence, Syarif Prasetyo, Tri Retnaningsih Soeprobowati, Tirtaduna Tirtaduna, Agus Purnomo, Sri Hartati, Umi Chodrijah, Prihatiningsih Prihatiningsih, Ari Wahyono, Jumari Jumari, Rossi Prabowo

The Philippine Agricultural Scientist

Lake Batur in Bangli Regency, Bali Province is a priority area for ecological quality restoration in Indonesia due to its vulnerability to environmental changes caused by diverse and intensive utilization. Agricultural activities, fisheries, and tourism affect the lake’s water quality, leading to the uncontrolled growth of water weeds such as water hyacinth (Pontederia crassipes). To determine the relationship between water quality, community activities, and water weed presence, water samples were collected from 9 locations consisting of residential, tourist, aquaculture, and agricultural areas. In situ analysis was then conducted to measure turbidity, pH, temperature, dissolved oxygen (DO), total dissolved …

Putting The Substance In Substantial Evidence: An Evidence-Based Approach To Flexible Drug Regulation, Emanuel Krebs, Tania M. Bubela, Melanie Mcphail, Christopher Mccabe, Dean A. Regier

Office of the Provost

For new drugs or indications, substantial evidence of clinical effectiveness is required for market authorization. In most jurisdictions, substantial evidence is not explicitly defined. Health regulators exercise discretion and are increasingly tolerant of earlier or less mature evidence. To align with flexible evidentiary standards, we argue for the adoption of a principle and, context-based approach to the evidence threshold. Our approach aims to balance the potential benefits and harms of accelerated authorization, low therapeutic value, and safety, based on a value of information (VoI) framework. In our VoI framework, substantial evidence exists when the expected net health value of further …

Connecting The Dots Between Narcissism And Fame Appeal: The Role Of Imaginary Audience Fantasies, Dara Greenwood, Qiana Archer, Sydney Ash, Julian Paul Keenan

Department of Biology Faculty Scholarship and Creative Works

Background:
Although prior research has found a significant association between narcissistic tendencies and fame interest, details regarding the particular nature of this relationship remain to be elucidated. The present online survey study (N = 355) replicates and extends prior research examining the links between two subtypes of narcissism and fame appeal. Specifically, we distinguish between grandiose and vulnerable narcissism, as predictors of Visibility, Status, and Prosocial domains of fame appeal. Further, we examine the potential mediating role of imaginary audience fantasies.

Participants and procedure:
Participants were drawn from a student sample (74% female-identifying) aged 18 to 25 who completed an …

Contraceptive Hormone Influences On The Role Of Primary Reinforcement And Reinforcer-Enhancement In Nicotine Self-Administration, Kathleen Robin Mcnealy

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Hormonal contraceptives contain a synthetic estrogen (e.g., ethinyl estradiol/EE) and/or a synthetic progestin (e.g., levonorgestrel/LEVO) and can exacerbate nicotine intake. However, whether altered intake reflects changes in nicotine reinforcement or enhancement by nicotine of co-occurring reinforcers is unknown. This reinforcer-enhancement effect is evidenced in rats when nicotine self-administration is increased by the presence of a visual stimulus (VS) reinforcer. This dissertation examined the effects of EE and LEVO on nicotine reinforcement and reinforcer-enhancement in ovary-intact female Sprague-Dawley rats. Rats were implanted with a jugular catheter and assigned to receive daily injections of EE (Vehicle, 0.125 [Low], or 0.18 [High] μg/day; …

Analysis Of Mybl1, E2f5, And, Maf1 Gene Relationships In Triple-Negative Breast Cancer, Kathy N. Dang

Theses (2016-Present)

Breast cancer is a highly heterogeneous disease, and is commonly diagnosed among many women and men around the world. Triple-negative breast cancer (TNBC) is a malignant form of breast cancer that currently remains a clinical challenge due to its difficult prognosis and treatment options. MYBL1, a gene that contributes to progression of tumors, was discovered in prior studies as an essential transcriptional factor that involves high levels of MYBL1 gene expression in TNBC. MYBL1 can be located on chromosome 8q locus, along with two other genes selected for this specific study, identified as E2F5 and MAF1. This chromosome of interest …

Tac4 Analyses Of Mybl1 Knockdown In Mda Mb231 Cells And Identification And Validation Of E2f4 And Tp53 Differential Gene Expression, Emmanuel Butler

Theses (2016-Present)

Triple-Negative Breast Cancer (TNBC) is known to be the most aggressive subtype of breast cancer, accounting for 15–20% of cases. In TNBC, three receptors commonly associated with breast cancer—estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptor 2 (HER2neu)—are under-expressed. Earlier studies identified MYBL1 as a major transcriptional activator overexpressed in TNBC, playing a critical role in tumor growth, differentiation and apoptosis. In previous research conducted by our lab, MYBL1 was knocked down in a TNBC cell line and the dataset saved for future use. In the current study, we re-analyzed the dataset and identified E2F4 and …

Neutrophil Exosomes: New Pathogenic Entities In Copd, Tiera L. Isom

Theses (2016-Present)

Chronic obstructive pulmonary disease (COPD) is a progressive respiratory disease that leads to chronic inflammation and, in turn, a high level of comorbidity and healthcare expense. An increasing body of evidence involves neutrophils, as well as their exosomes, in lung damage exacerbation via the protease-mediated degradation of the extracellular matrix (ECM). We hypothesize that the PMN-exosomes would be significantly increased in the active smoker COPD patient and that they would enhance disease progression by augmenting the proteolytic cascade and tissue damage. This multi-center clinical study included a total of 46 veterans separated into four cohorts: active smoking COPD patients, COPD …