Digital Commons Network^™

Crispr-Induced Mutagenesis Of Arabidopsis Thaliana Gene Apetala3, Hazel Frans, Tara Phelps-Durr

SACAD: Scholarly Activities

This project aims to create CRISPR-CAS9 mutations in the APETELA3 (AP3) gene of the model plant Arabidopsis thaliana. AP3 is a class B gene critical to the petal and stamen development of Arabidopsis flowers. AP3 is defined in a MADS domain, which binds directly to DNA and may be responsible for the expression of the CaRG-box genes. AP3 works in conjunction with PISTILLATA (PI), AGAMOUS (AG), APETALA1 (AP1), and SEPALLATA (SEP) genes to specify the development in the second and third whorls of the flower. While several alleles of AP3 already exist, these alleles are strong alleles that knockout gene …

Optimizing Transcription Efficiency Through Mutations In The T7 Rna Polymerase, Claire N. Novak

Honors Program: Senior Projects (Public)

The production of RNA is vital for many commercial and research purposes, with one of the more notable reasons being vaccine production. The RNA polymerase, derived from T7 bacteriophage, is commonly used for RNA production due to its ability to produce full-length RNA transcripts. However, researchers have faced limitations in optimizing this polymerase. For example, the polymerase generates heterogeneous RNA products that require additional purification steps during RNA production. Here, we selected three amino acid point substitutions based on a recent publication from Moderna that showed increased RNA purity compared to the wild type T7 RNA polymerase sequence. We created …

Painting Rich Six-Dimensional Pictures Using Polarized Fluorescence Microscopy, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

No abstract provided.

The Prillwitz Mammoth: Taxonomy, Sex, Age, & Delayed Skeletal Maturation Of A Geologically Young Mammoth, Anna Rybachek

Honors Theses

The Prillwitz mammoth, a nearly complete mammoth skeleton found in Southwest Michigan in 1962, is one of the geologically youngest mammoths from North America (AMS C-14 date: 11,300 ± 100 BP). Bones are well preserved; some long bones show post-mortem flaking, scratches, or grooves of unknown origin. All long-bone epiphyses remain unfused except the distal humerus, suggesting it was < 28 years old at death. In contrast, the skull and mandible preserve M3s and m3s, respectively, that are in mid-wear, indicating an Asian Elephant Year dental age of ~45-50 years. Long bone size and inferred shoulder height are consistent with the size of a large woolly mammoth (Mammuthus primigenius) or small to mid-sized Columbian mammoth (Mammuthus columbi), but dental evidence is most consistent with M. columbi.

Pelvic dimensions suggest it is a male. The ≥ 17-year mismatch between age inferred from tooth progression and wear (~45 Asian Elephant Years) and age inferred from epiphyseal fusion (< ~28 Asian EY if male) may indicate either substantially accelerated dental progression or delayed skeletal maturation for this mammoth; we deem the latter more likely. Based on modern analogs, delayed maturation could have been due to severe malnutrition, hormonal imbalance, illness, or genetic factors. Given the geologically young age of the Prillwitz mammoth (likely near the time of mammoth extinction), we speculate that delayed skeletal maturation may have been attributed to environmental stressors near the end of the Pleistocene. Further work is warranted on very late mammoths.

Limosilactobacillus Reuteri Promotes The Expression And Secretion Of Enteroendocrine- And Enterocyte-Derived Hormones, Sara C Di Rienzi, Heather A Danhof, Micah D Forshee, Ari Roberts, Robert A Britton

Faculty, Staff and Students Publications

Intestinal microbes can beneficially impact host physiology, prompting investigations into the therapeutic usage of such microbes in a range of diseases. For example, human intestinal microbe Limosilactobacillus reuteri strains ATCC PTA 6475 and DSM 17938 are being considered for use for intestinal ailments, including colic, infection, and inflammation, as well as for non-intestinal ailments, including osteoporosis, wound healing, and autism spectrum disorder. While many of their beneficial properties are attributed to suppressing inflammatory responses, we postulated that L. reuteri may also regulate intestinal hormones to affect physiology within and outside of the gut. To determine if L. reuteri secreted factors …

Uterine Pgrmc2 Deficiency Attenuates Endometrial Hyperplasia And Cancer And Prolongs Lifespan In A Pten Loss-Of-Function-Induced Cancer Model, Nicole C Kelp, Cindy A Pru, Sandeep Paudel, John P Lydon, J Julie Kim, John J Peluso, James K Pru

Faculty, Staff and Students Publications

The expression of members of the progesterone receptor membrane component (PGRMC) family, particularly PGRMC1, is elevated in diverse types of cancers, particularly those of the female reproductive system. While xenograft tumor studies using human transformed cell lines in immunocompromised mice have suggested that PGRMC1 enhances tumor growth and chemoresistance, the exact role of members of the PGRMC family in cancer development in vivo remains unclear. In this study, we examined the effect of deleting Pgrmc2 on the development of endometrial hyperplasia and cancer using a murine phosphatase and tensin homologue (Pten) conditional loss-of-function model. We previously established that …

Galectin-1 Inhibition As A Strategy For Malignant Peripheral Nerve Sheath Tumor Treatment, Hsiao-Chi Wang, Keila E Torres, Roger Xia, Marcio H Malogolowkin, Ssu-Wei Hsu, Ching-Hsien Chen, Tsung-Chieh Shih

Faculty, Staff and Student Publications

Neurofibromatosis type 1 (NF1) is an inherited disorder that predisposes individuals to malignant peripheral nerve sheath tumors (MPNSTs), a highly aggressive sarcoma with limited treatment options and poor prognosis. This study explores the potential of targeting the interaction between Galectin-1 and Ras as a novel therapeutic strategy for MPNSTs. Through molecular docking, we identified critical residues involved in the Galectin-1 and H-Ras interaction. We developed LLS30, a compound designed to target this Ras-binding pocket on Galectin-1, and tested its efficacy. LLS30 effectively disrupted the Galectin-1/Ras interaction, causing Ras delocalization from the plasma membrane and inhibiting Ras signaling. In vitro experiments …

Oral Decitabine Cedazuridine With And Without Venetoclax In Higher-Risk Myelodysplastic Syndromes Or Chronic Myelomonocytic Leukemia: A Propensity Score-Matched Study, Alex Bataller, Koji Sasaki, Samuel Urrutia, Guillermo Montalban-Bravo, Alexandre Bazinet, Kelly Chien, Danielle Hammond, Ian M Bouligny, Mahesh Swaminathan, Ghayas Issa, Nicholas Short, Naval Daver, Courtney D Dinardo, Tapan Kadia, Elias Jabbour, Farhad Ravandi, Gail J Roboz, Michael Savona, Elizabeth A Griffiths, James Mccloskey, Olatoyosi Odenike, Aram Oganesian, Harold N Keer, Mohammad Azab, Hagop Kantarjian, Guillermo Garcia-Manero

Faculty, Staff and Student Publications

Hypomethylating agents (HMA) are indicated in the treatment of higher-risk myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML). The combination of hypomethylating agents with venetoclax (Ven) has demonstrated promising results in these diseases, although randomized clinical trials are needed for validation. In this retrospective study, we compared two matched cohorts of patients with MDS or CMML: one receiving oral decitabine-cedazuridine (DEC-C, n = 73) and one receiving DEC-C and Ven (DEC-C-Ven, n = 51), in three contemporary clinical trials. The aim is to determine the impact of the addition of Ven to HMA in MDS and CMML. Individuals were matched …

Critical Thinking Skills: Improvement Through Learning Ownership-Pbl Approach On Students, Ulfa Risqillah, Dwi Listyorini, Sitoresmi Prabaningtyas, Siti Salina Mustakim

Jurnal Pendidikan Sains

Through a method called "Learning Ownership," students actively participate in the educational process and comprehend the context, objectives, and applications of the content they have learned. A learner can customize each educational encounter to align with their present and future learning goals once they have control over their learning processes. The skills and practices required for ownership are highly versatile and applicable in various educational environments. The aim of this study was to unveil the effect of the learning ownership-PBL model on students' critical thinking skills. The sampling technique used in this research was random sampling, which consisted of 2 …

Impact Of Climate Change On The Diversity And Abundance Of Wintering Birds At A Northern African Wetland - Case Study Of Oglat Eddaira Wetland (Naama, Western Algeria), Ahmed Toufik Youcefi, Gouacem Ali, Litim Kouider, Haddi Leila, Messaoui Fatima

Journal of Bioresource Management

The Mediterranean regions have been classified as the areas of the world most susceptible to the effects of environmental change. These regions are sensitive to drought and rising temperatures and with heightened aridity in the next several years. The purpose of this paper is to study the effects of climate change on the diversity and abundance of wintering birds in the Oglat Eddaira wetland located in western Algeria and classified in the Ramsar list. This study is based on the comparison between some indices of biodiversity: species richness, abundance, Shannon-Weiner and Simpson Index, during the period 2015 to 2024 and …

Aerial Phytomass And Carbon Sequestrated By The Kitshanga Forest Massif In The Wamba Valley In Dr-Congo, Mayanu Pemba, Ntalakwa Makolo, Kidikwadi Tango, Lubini Ayingweu

Journal of Bioresource Management

Aerial phytomass and carbon sequestrated by the Kitshanga forest massif in the Wamba valley in DR-Congo. The general objective was to evaluate the aerial phytomass and the carbon contained in the ligneous tissues of the trees. Specifically, the research aims to: (i) inventory all individuals in the Kitshanga gallery forest; (ii) identify the species in the study area; (iii) determine the floristic composition and density of the stand studied and (iv) evaluate the phytomass and the carbon sequestered by this vegetation. Inventory devices full on 1 hectare subdivided by 400 m² to obtain 25 plots which constitute the size of …

Study On Predisposing Factors And Control Measures Against Cryptosporidiosis In Buffalo Calves At Cattle Colony Hyderabad, Sindh, Pakistan, Ali Ul Murtaza, Abdul Latif Bhutto, Jam Kashif Zaman, Mansoor Tariq, Amjad Hussain Mirani, Riaz Ahmed Laghari, Pervez Ahmed Khoso, Muhammad Mohsen Rahimoon, Abdul Saboor, Fayaz Hussain

Journal of Bioresource Management

The current study looked at the variables affecting the frequency of Cryptosporidium Parvum in buffalo calves at the Hyderabad Cattle Colony. From calves, 100 fecal samples were taken in total, preserved, and analyzed using direct smear and Ziehl-Neelsen staining methods. 74 percent of the samples tested positive for Cryptosporidium Parvum. Disease rates were higher in Nili Ravi calves (86 %) compared to Kundhi calves (62 %), with a statistically significant difference (p

Review On Biological Management Of Bactrocera Zonata Through Pathogenic Activity Of Beauveria Bassiana, Sana Ullah, Hafiz Muhammad Sajid Ali, Syed Husnain Kumail Naqvi, Muhammad Ziaullah, Farhan Ali, Sana Sarfaraz, Mamoona Kanwal

Journal of Bioresource Management

Bactrocera zonata is the destructive pest of peach, mango, and banana by causing significant economic losses. It reduces fruit quality which directly causes decline in export of these economical fruits. Many management strategies were carried to suppress the population of this invasive pest. The use of chemical insecticides in the orchard for control of B. zonata is restricted because insecticides cause several complications in fruit health and makes fruits unfit for human consumption. Due these drawbacks of chemical control, Biological control strategies are preferred for better management of B. zonata. Entomopathogenic fungi are potential bio-control agent against many insect …

Nitrogen Application Enhances The Radiation Use Efficiency And Quality Of Cotton (Gossipum Hirsutum L.) Under Divergent Agro-Ecological Conditions Of Pakistan, Amjed Ali, Safdar Hussain, Wajid Nazeer, Muhammad Sarwar, Masood Qadir, Mubashar Nadeem, Javeria Sherani

Journal of Bioresource Management

Cotton being a cash crop plays a pivotal role in the economy of Pakistan. Climate change has a pronounced impact on radiation use efficiency and quality of cotton fiber. To find out how nitrogen affected the radiation use efficiency and fiber quality of cotton cultivars in Punjab, a field study using a split-plot design was carried out. The study under representation was conducted in three different locations over the course of two years (2016 and 2017): Adaptive Research Farm in Dera Ghazi Khan, The Regional Agriculture Research Institute, Bahawalpur and The College of Agriculture, University of Sargodha. Three cotton cultivars …

Avifauna Of Kheri Morat National Park: Role Of Agricultural Habitat In Maintaining The Diversity Of Birds In The Presence Of Threats In Adjacent Forest Habitat, Syed Ali Hasnain Mosvi, Saeed Azam, Abdullah Ibrahim, Saleha Abbasi, Nadeem Munawar, Muhammad Arslan Asadi

Journal of Bioresource Management

Increasing land use for agricultural practices is commonly perceived as a threat to the avian diversity. However, a number of avian species depend on these croplands for food, water, and shelter. The present study was conducted in Khairi National Park, Pakistan, for the discourse of awareness gaps about the avifaunal diversity of the protected area and to compare the bird’s diversity in the agriculture fields and native forests. For the current study, the habitat was divided into two habitat categories, i.e., forest and croplands. The sampling was executed between February 2014 and February 2017. Stratified random sampling was used to …