Digital Commons Network^™

Genomic Surveillance Of Guillain–Barré Syndrome-Associated Genes In Campylobacter Jejuni Across Human And Non-Human Reservoirs, Emma Doughty

Biological Sciences

Campylobacter jejuni is a leading cause of foodborne illness and a major trigger of Guillain–Barré Syndrome (GBS), an autoimmune neuropathy caused by molecular mimicry between bacterial lipooligosaccharides (LOS) and host nerve gangliosides. While prior genomic studies have focused primarily on human clinical isolates, the prevalence and distribution of GBS-associated genes in non-human reservoirs remain poorly characterized. This study used genomic datasets derived from analyses of human and non-human C. jejuni isolates to assess the distribution of GBS-associated genes, with a focus on the neuABC sialylation pathway. Results demonstrate that these genes are present in both host types, across multiple geographic …

Alternative Abl1 Isoform Expression In Oncogenic Translocations: Implications For Cancer Progression, Jessica Teague

Biological Sciences

Abstract (also on poster): Previous studies in our lab show that an alternate isoform of Bruton’s tyrosine kinase, BTK-C, is required for the survival of several epithelial-derived cancer cells. BTK-C is transcribed from an alternative promoter and includes a 34-amino-acid N- terminal extension with a palmitoylation sequence that alters its subcellular localization. Nine other human kinases have similar alternative N-termini (ANT), often producing palmitoylated or non-palmitoylated isoforms from different transcriptional start sites. Among these, ABL1 is a known oncogenic kinase and key drug target. Translocation mutations at the 5′ end of ABL1 are a major cause of adult leukemias. This …

High Throughput Phenomics Pipeline For Pulse Crop Nutritional Breeding, Amod Udayanga Madurapperumage

All Dissertations

Dry pea (Pisum sativum L.), lentil (Lens culinaris Medik.), and chickpea (Cicer arietinum L.) are major pulse crops valued for their high nutritional composition and importance to global food systems. Pulses are rich in carbohydrates, protein, and essential minerals, making them ideal whole foods and critical contributors to food and nutrition security. Due to these advantages, pulse breeding programs are increasingly focusing on enhancing nutritional traits, such as protein quality, amino acid balance, and micronutrient density, through the process of biofortification. However, improvement of agronomic traits remains equally essential. Characteristics such as plant height, standability, stress tolerance, …

Integrating Incentive Design And Spatial Prioritization For Climate-Smart Forestry Adoption In South Carolina, United States, Miah Maye Pormon

All Dissertations

Forests provide essential ecosystem services, including carbon sequestration, water regulation, timber production, and habitat provision. However, increasing development pressures and land-use changes threaten forest persistence and long-run ecosystem service provision. Climate-smart forestry (CSF) practices, such as improved forest management and extended rotation, offer opportunities to enhance carbon storage, forest resilience, and economic livelihoods. The effectiveness of these practices depends on forest owners’ participation and the strategic allocation of financial resources or incentives. This dissertation develops an integrated framework that combines behavioral economic analysis and mapping to improve the design of current incentive programs. The first chapter employs the Contingent Valuation …

Arkansas Soybean Research Studies 2024, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

The 2024 Arkansas Soybean Research Studies includes research reports on topics pertaining to soybean across several disciplines from breeding to post-harvest processing. Research reports contained in this publication may represent preliminary or only data from a single year or limited results; therefore, these results should not be used as a basis for long-term recommendations. Several research reports in this publication will appear in other University of Arkansas Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas soybean producers of the research being …

Examining Pathogenic Vibrio Abundance And Environmental Controls Across Macroalgae, Sediment, And Oysters, Alexandra H. Geisser, Abigail K. Scro, Roxanna Smolowitz, Robinson W. Fulweiler

Arts & Sciences Faculty Publications

Climate change is accelerating the geographic spread and abundance of pathogenic Vibrio species in coastal waters, posing growing threats to ecosystems and human health. We investigated the abundance and pathogenic gene presence of V. vulnificus and V. parahaemolyticus across three common estuarine substrates (macroalgae, sediment, and oysters) in a temperate estuary. Macroalgae consistently harbored the highest Vibrio counts in conjunction with elevated pathogenic gene frequencies, while oysters contained the lowest. Environmental conditions related to Vibrio abundance were substrate and Vibrio species specific. Temperature and salinity were most closely related to pathogenic Vibrio abundance in oysters. In contrast, pathogenic Vibrio abundance …

Biohybrid Robotic Jellyfish For Swimming-Enhanced Vertical Ocean Profiling, Kelsi M. Rutledge, Sean Colin, John H. Costello, Noa Yoder, Simon R. Anuszczyk, Kelly R. Sutherland, Brad L. Gemmell, John O. Dabiri

Arts & Sciences Faculty Publications

Ocean monitoring is essential for understanding climate change and marine ecosystem dynamics, yet achieving comprehensive global coverage remains a challenge in oceanography. Current technologies face limitations in cost, power, hardware, and depth capacity that restrict widespread monitoring capabilities. Here we show that biohybrid robotic jellyfish (Aurelia aurita) can serve as autonomous vertical ocean profilers by integrating microcontrollers with positively buoyant sensor payloads, achieving controlled vertical-profiling capabilities. Laboratory experiments demonstrated repeatable up–down trajectories, quantified force balance limits, and identified predictable, size-dependent descent swimming speeds. Field deployments in Massachusetts coastal waters and the open ocean off the Florida Keys demonstrated field operation …

Mechanisms Of Synergy Between Nanosecond Pulsed Electric Field (Nspef) And Cold Atmospheric Pressure Plasma Discharge (Cap) In Pancreatic Cancer: The Role Of Ros-Mediated Oxidative Stress And Apoptosis, Zobia Minhas

Biomedical Sciences Theses & Dissertations

Pancreatic ductal adenocarcinoma is a highly lethal malignancy characterized by poor prognosis and resistance to conventional therapies. Novel treatment strategies are needed to overcome this resistance. Non-thermal physical modalities, including nanosecond pulsed electric field (nsPEF) and cold atmospheric pressure plasma discharge (CAP), have emerged as potential anticancer approaches; however, the mechanisms underlying their combined effects remain unclear.

This study evaluated whether nsPEF enhances CAP-induced cytotoxicity and investigated the associated molecular mechanisms using the murine Pan02 pancreatic cancer cell line. Cell viability was assessed by WST-1 metabolic activity assays and electric cell–substrate impedance sensing (ECIS). Intracellular reactive oxygen species (ROS) were …

Low Methane Emissions From Nutrient-Rich Former River Channels In A Hydrologically Managed Dryland Delta, Siena Stassi, Jude A. Benavides, Chu-Lin Cheng, Marcelo Gomes Da Silva, Nicholas E. Ray, Nathan O. Barros, Rafael M. Almeida

School of Earth, Environmental, & Marine Sciences Faculty Publications

Anthropogenic stresses in dryland regions can alter hydrology, salinization, and biogeochemical processes. How these pressures influence methane (CH4) cycling and emissions from aquatic ecosystems remains poorly understood. Over the course of 1 year, we measured diffusive and ebullitive CH4 emissions from resacas—shallow, hydrologically managed distributary channels prevalent throughout the Lower Rio Grande Valley along the US–Mexico border. Despite warm, nutrient-rich waters, CH4 emissions from resacas were low, and more comparable to rates observed in estuarine systems. CH4 fluxes were positively related to temperature but negatively related to salinity and rainfall. Elevated salinity, driven by evaporation and source water from the …

Landscape Connectivity And Wildlife Access To Water Across An International Border: Barriers And Opportunities For Facilitating Transboundary Movement, Bogdan Chivoiu, Erin L. Koen, Michael J. Osland, Christopher A. Gabler, Jerald T. Garrett, Ernesto Reyes, Stephanie A. Bilodeau, Mitch A. Sternberg, Jude A. Benavides, James Martinez

School of Earth, Environmental, & Marine Sciences Faculty Publications

Rapid global acceleration in the construction of physical barriers along international borders has greatly influenced biodiversity and animal movement. Physical barriers can fragment landscapes, hinder access to essential resources, impact long-distance migrations, and inhibit dispersal and gene flow. The effects of physical barriers on animal movement and landscape connectivity can be exacerbated in dryland environments where access to water is a limiting factor. In recent decades, the construction of border barrier infrastructure has accelerated along the international boundary between the United States and Mexico. Here, we used a landscape connectivity model to investigate the effects of barriers on wildlife access …

Ecological Insights And Management Implications Of The Global Migratory Connectivity Of Green Turtles, Jaime Restrepo, Dina Nisthar, Harris Wei-Khang Heng, Lily K. Bentley, Corrie Curtice, Sarah Deland, Ei Fujioka, Patrick N. Halpin, Sarah K. Poulin, Roldán A. Valverde

School of Earth, Environmental, & Marine Sciences Faculty Publications

Aim

Green turtles are a widely distributed and highly migratory species; despite extensive data on their movement, there is no species-specific global synthesis on the subject. Based on three decades of published literature and building on previous global analyses, we developed a global network model of migratory connectivity for green turtles to better understand their spatial biology.

Location

Global.

Time Period

1990–2022.

Major Taxa Studied

Green Sea Turtle (Chelonia mydas).

Methods

We conducted a structured literature review extracting georeferenced information on the movement of green turtles from 1990 to 2022, aggregating this information into a single connectivity model, …

Post-Exercise Vitals Recovery Response In Barrel Horses: A Field Study On Cooling Methods Used By Competitors, Lauran E. Hetrick

Electronic Theses and Dissertations

Barrel racing demands peak physical performance from equine athletes, yet limited research evaluates the field-based strategies used to optimize equine recovery at competition. This study assessed recovery management approaches used by competitors to elucidate their influence of physiological recovery by measuring heart rate, respiratory rate, and body temperature during a high-profile barrel race competition. Physiological data were collected at baseline, immediately post-exercise, and during a 15–30-minute cooldown period to characterize recovery under real-world conditions. Significant increases in HR, RR, and BT occurred from baseline to post-run (p < 0.001), followed by reductions during cooldown (p < 0.01), though values remained elevated (p < 0.05). Rinsing reduced HR and RR (p < 0.01), while shade and fans decreased RR and variably influenced HR. Noise, as a confounding variable, decreased HR but increased BT (p < 0.001). Ultimately, these results emphasize multimodal recovery approaches in competitive equine management in the field.

Body Size Variation And Floral Associations Of The American Bumble Bee, Bombus Pensylvanicus, In East Texas, Sabekun Nahar

Electronic Theses and Dissertations

The American bumble bee, Bombus pensylvanicus, is an important pollinator species that was once widespread in much of North America but has experienced population declines in recent decades in some regions. As a species of conservation concern, it is important to understand how body size varies in response to environmental conditions and to identify the key floral resources that support this species. I examined body size variation in East Texas and floral resource use of B. pensylvanicus across Texas. In the first chapter, body size variation among queens, workers, and males was quantified using thorax width and size variation …

Potential Impact Of Parasites In The Transmission Of Chronic Wasting Disease, Paulina Soto, Rodrigo Morales

Faculty, Staff and Student Publications

No abstract provided.

Corrigendum To 'Risk Factors For Bronchiolitis Obliterans Syndrome After Initial Detection Of Pulmonary Impairment After Hematopoietic Cell Transplantation' [Transplantation And Cellular Therapy 29/3 (2023) 204-204], Mansour Alkhunaizi, Badar Patel, Luis Bueno, Neel Bhan, Tahreem Ahmed, Muhammad H Arain, Rima Saliba, Gabriela Rondon, Burton F Dickey, Lara Bashoura, David E Ost, Liang Li, Shikun Wang, Elizabeth Shpall, Richard E Champlin, Rohtesh Mehta, Uday R Popat, Chitra Hosing, Amin M Alousi, Ajay Sheshadri

Faculty, Staff and Student Publications

No abstract provided.

Optimized Ultrasound Imaging Of Phase-Change Nanodroplets, Charles R Dyall, Dmitry Nevozhay, Andy Liu, Trevor M Mitcham, George J Lu, Konstantin V Sokolov, Richard R Bouchard

Faculty, Staff and Student Publications

Phase-change nanodroplets (PCNDs) continue to generate significant research interest due to their potential to extravasate into tissue, to be targeted for molecular imaging and drug delivery, and to undergo an induced phase-change to "activated" microbubbles (MBs) for ultrasound (US) imaging. To accurately quantify molecular markers, however, one assumes a consistent proportion of PCNDs in a region of interest (ROI) are stably activated and imaged. Herein we present a framework for developing a diagnostic sequence that is optimized for PCND activation uniformity, contrast, and acquisition time. To develop this framework, activation was examined at three scales of increasing complexity: single, adjacent, …

Disruption Of Energy Homeostasis By Chemical Mixtures Containing Persistent Pollutants In Hepg2 And C2c12 Cells, Morgan Jacobellis

All Theses

The Environmental Influences on Child Health Outcomes Fetal Growth Studies (ECHO-FGS) birth cohort data was used to identify chemical mixtures associated with adverse outcomes. This biomonitoring study associated specific chemical mixtures in maternal serum with low birth weight or childhood obesity (Pearce et al., 2021). Mixture Profiles 1, 3, and 4, comprised of different observed concentrations of DDE, PCB153, BDE47, and PFOS were prioritized. Mixtures were tested to determine whether they perturbed mitochondrial metabolism and energy sensing pathways. The purpose of this study was to elucidate a potential mechanism linking maternal exposure to mixtures containing persistent organic pollutants (POPs) to …

Quantifying Macrophyte Ecosystem Services And Improving Environmental Nonprofit Messaging, Peyton R. Hughey

All Theses

Environmental nonprofits are essential to the management and restoration of our natural spaces. They are also tasked with engaging the public via outreach, education, and advocacy. As natural spaces are degraded, the ability of environmental nonprofits to deliver on their missions will become increasingly important. This study aimed to improve two approaches to environmental nonprofits’ missions: ecological restoration and social media communication.

Environmental nonprofits operate with limited resources, and ecological restoration can be an exhaustive task; therefore, we explore functional restoration as a tool. In this study, we focus on the use of functional, trait-based restoration to restore ecological function …

Mining The Usda Snap Bean Association Panel For Resilience: Genetic Dissection Of Heat And Disease Tolerance In Snap Bean, Atiya F. Khan

All Theses

Snap bean (Phaseolus vulgaris L.) is an economically and nutritionally important vegetable crop whose production is increasingly threatened by rising temperatures and emerging bacterial diseases, particularly in the southeastern United States. Heat stress during reproductive development can significantly reduce yield, while newly emerging pathogens such as Pseudomonas capsici present additional risks to crop productivity. This study leveraged the USDA Snap Bean Association Panel (SnAP) to investigate the genetic basis of heat tolerance and disease resistance and to identify germplasm and genomic regions useful for breeding resilient cultivars.

To evaluate heat tolerance, a subset of the SnAP panel was …

Polydactyl Precursors: Insights Into Early Tetrapod Locomotion From Polydactyl American Alligators, Trevor P. Brewington

All Theses

Several of the earliest tetrapod lineages exhibited polydactyl limbs, with seven or eight digits per limb rather than the typical five or fewer seen in extant lineages. How these digit configurations might have been used during terrestrial locomotion is unclear, given the lack of living models in which their performance can be studied and limited evidence from fossil trackways. However, polydactyly occasionally appears in natural populations of American alligators (Alligator mississippiensis), animals with body plans that broadly resemble those of early tetrapods. To test how polydactyl limbs similar to those of early tetrapods might have functioned on land, …

Biology, Ecology And Control Of Eye Gnats In An Open Pine Ecosystem, Leela Hospach

All Theses

Eye gnats of the genus Liohippelates are bothersome flies of the family Chloropidae. They breed in moist, disturbed, sandy soils that contain organic matter, and have been implicated as vectors of conjunctivitis, bovine mastitis, and yaws. We conducted research into some basic biological and ecological characteristics. Using wild-caught gnats in a temperature-controlled incubator, we found that their activity peaks between 27°C and 41°C. Trapping at different heights above the forest floor in an open-pine savannah, we captured most gnats at 7.5 m off the ground. This may have to do with the attractiveness of the rotten egg lures we used, …

An Investigation Of Entamoeba Histolytica Chitin Synthases And Other Encystation Factors In Early Encystation, John N. Talledo

All Theses

Entamoeba histolytica is a food-and-water-borne human protozoan pathogen that relies on a two-stage life cycle for successful proliferation outside of the host. E. histolytica exists in its dormant cyst form while outside of its host and converts to the motile trophozoite (amoeba) form in the small intestine. The trophozoites colonize the large intestine to cause symptomatic disease in ~10% of infections, resulting in ~100 million cases of amoebic dysentery each year. Trophozoites can convert back to the cyst form, which is shed in feces to continue the infection cycle.

Currently, there is a lack of understanding of the exact molecular …

Identification, Pathogenicity, And Fungicide Sensitivity Of Itersonilia Perplexans Associated With Petal Spot Of Gerbera Cut Flowers In Colombia, Matheus Beger

All Theses

Petal diseases significantly affect gerbera cut flower production, as minor visual damage renders flowers unmarketable. Among those pathogens causing petal spots, Itersonilia perplexans affects some Asteraceae plants. However, this pathogen's impact on Colombian gerbera remains poorly studied. This study was designed to fill four gaps: molecular identification of Colombian isolates, confirmation of pathogenicity, standardized culture media, and fungicide sensitivity data. Four objectives were pursued: (i) isolate and identify the causal agent through sequencing the Internal Transcribed Spacer, Small Subunit and Large Subunit from the rDNA; (ii) evaluate pathogenicity on detached gerbera petals; (iii) identify optimal culture media for I. perplexans …

Developing Aero-Floating Treatment Wetland (Aeroftw) Scaffolds: A Non-Plastic Alternative To Conventional Plastic-Based Ftws, Ada C. Montoya Gomez

All Theses

This research was conducted to explore the possibility of constructing floating treatment wetlands (FTWs) without using plastic materials. FTWs are floating platforms that hold plants on the water’s surface, letting roots dangle within the water column, to help remove contaminants from contaminated water, especially in stormwater ponds. Many conventional FTWs are made with plastic components, such as mats and plastic cup containers, which can release microplastics into the environment over time. The goal of this project was to design and test a new type of FTW that does not depend on plastics to support FTW flotation. The study took place …

From Invisible Injury To Measurable Impact: Exploring Associations Between Peripheral Biomarkers Of Neuroinflammation And Mental Health Outcomes After Mtbi, Lauren Leggett Smith

Psychology Theses & Dissertations

Mild traumatic brain injury (mTBI), often referred to as a “silent epidemic,” accounts for the majority of traumatic brain injuries worldwide and is increasingly linked to persistent psychiatric outcomes. Despite its prevalence, mTBI remains understudied due to diagnostic challenges, underreporting, and misperceptions of its clinical significance. The present study examined whether peripheral biomarkers of neuroinflammation and astroglial injury—tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and glial fibrillary acidic protein (GFAP)— mediate associations between mTBI history and psychiatric symptom severity, including depression, anxiety, and posttraumatic stress disorder (PTSD). Participants were 138 traumaexposed adults recruited from an urban university and surrounding community, with …

Analyzing Criteria Air Pollutants And Meteorological Parameters In The Rio Grande Valley, Laredo, And Corpus Christi Region Of South Texas, Angela Garcia

Theses and Dissertations

Recently, the Rio Grande Valley has experienced significant population growth due to economic opportunities, cross-border commerce, SpaceX, and the Rio Grande and Texas liquid natural export terminal presence. The Rio Grande Valley has an estimated population of 1,368,723. This region is largely understudied, along with nearby urban centers such as Laredo and Corpus Christi. The Rio Grande Valley and Laredo regions are a concern due to their population growth and heavy cross-border transportation. Data from the Texas Commission of Environmental Quality Continuous Ambient Monitoring Sites was collected from January 1st , 2024, to December 31st, 2024. Statistical analysis, such as …

Evaluation Of Palm Oil As An Ingredient In Finishing Beef Cattle Diets On Performance, Carcass Characteristics, And Meat Quality, And The Composition Of Spayed Heifers To Intact Heifers Fed Melengestrol Acetate On Feedlot Performance And Carcass Characteristics, Tate S. Johnson

Department of Animal Science: Dissertations, Theses, and Student Research

Heifers represent a large proportion of cattle placed in feedlots, making pregnancy management an important consideration for maintaining performance and carcass value. Melengestrol acetate (MGA) is commonly included in feedlot diets to suppress estrus and prevent pregnancy in intact heifers during the finishing period. Alternatively, spaying has historically been utilized as a management practice to allow heifers to graze alongside breeding cattle without the risk of pregnancy. Despite its historical use, there is limited contemporary research comparing the growth performance and carcass characteristics of spayed heifers with intact heifers managed with MGA under modern feedlot conditions. Therefore, a commercial study …

Understanding Roma Oppression In Europe Through Humantarian Forensic Analysis, Christal L. Quinn

Department of Anthropology: Theses and Student Research

This thesis examines the marginalization of Roma people in Europe, and how humanitarian forensic practices may help Roma communities in their quest for justice and recognition. The study draws exclusively on secondary information to examine the social, political, and legal issues affecting the Roma communities, who have endured centuries of institutional discrimination, marginalization, and violence. This thesis identifies ways in which systematic oppression continues to limit Roma communities’ access to education, housing, healthcare, and legal protection through a review of relevant literature and applicable theoretical frameworks. The study also examines the relevance of forensic science, particularly its humanitarian applications, in …

Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Specialty crops such as vegetables are some of the most susceptible to yield losses and increased management difficulty over time due to pests. One of the potential solutions to this problem, which aims at ensuring the sustainability of the sector, is using conservation biological control practices such as intercropping to increase diversity, while maintaining the economic feasibility and profitability of the system. This study was conducted to evaluate the impact of this management practice using Bell pepper (Capsicum annuum var. King Arthur) as the main crop, intercropped with flowers including zinnia (Zinnia elegans var. Benary’s Giant), sunflower ( …

Unravelling Taxonomic Complexity In Elusive Cetaceans: Mitogenome Insights Into Evolutionary History And Cryptic Diversity Of Bryde's Whales, Dominique Paynee, Paulette Bloomer, Gwenith Penry, Simon Elwen, Ada Natoli, Vaisakh Dhanalakshmy, Thyago Cardoso, Els Vermeulen

All Works

Cetaceans, despite their ability to cross wide distances, enabling mostly high levels of gene flow, can develop unique genetic lineages driven by variations in life-history traits, diverse migratory routes and local adaptation, complicating taxonomy and delineation of conservation units. Comprehensive genetic data are essential to overcome the challenges of obtaining suitable sample sizes from wild cetaceans while untangling cryptic species diversity. The Bryde's whale (Balaenoptera edeni) has a complicated discovery and taxonomic history that hinders accurate conservation assessments. In southern Africa, two populations are recognised: the inshore (SAi) and the offshore southeast Atlantic population (SEA). This study examines their phylogenetic …