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Developing Training And Streamlining Training Management Process For Smithfield Fire Department Employees In Aha, Naemt, And Aap Certifications, Michael Christopher Popa Ii

Undergraduate Honors Capstone Projects

The rapid operational expansion of the Smithfield Fire Department and EMS (SFD)—marked by a 300% increase in call volume and a transition to a fully professional, independent paramedic service—exposed critical systemic inefficiencies in clinical preparedness and personnel credentialing. This project addresses the "HALO Paradox," wherein high-acuity, low-occurrence emergencies require expert-level proficiency that cannot be maintained through call volume alone. Historically, the agency relied on fragmented, external continuing education (CE) that incurred high financial costs, lacked equipment standardization, and created administrative bottlenecks during biannual recertification cycles. The central objective of this capstone was to engineer a sustainable, in-house training infrastructure that …

The Hitchhiker's Guide To The Host: Fleas And The History Of Pikas In North America, Josh Pletcher

All NMU Master's Theses

Parasite phylogenetics can offer windows into the cryptic evolutionary history of hosts, often highlighting events that are not recorded by the phylogenetics of the host. Pikas (small lagomorphs) host a diverse parasite community comprising endoparasites (e.g., coccidians, nematodes, and cestodes) and ectoparasites (e.g., fleas and mites). Phylogenetic analysis of pika endoparasites has revealed pikas evolved in Asia and colonized North America in two waves, and that contact between these two pika lineages allowed parasites to be transferred from one lineage to another and to survive the extinction of their original host. To uncover these cryptic histories, understanding the biogeography of …

Dfend: A Nutrition Education Intervention To Improve Food Budgeting, Shopping, And Meal Preparation Skills Among Adults In Arkansas, Samantha K. Edwards

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Preventive strategies targeting modifiable lifestyle factors related to food, nutrition, and health are needed as chronic health conditions continue to rise. Nutrition literacy refers to having the knowledge and skills needed to obtain, understand, and apply nutrition information to make informed dietary decisions. Without adequate nutrition education and food security, vulnerable populations are at risk of malnutrition and poor dietary behaviors. Educational interventions that increase food literacy can help improve dietary choices and food selection skills.

The purpose of this creative project was to develop a nutrition education toolkit for Extension agents to implement among rural populations with low health …

Leveraging Historic Collections: Investigating Morphometric Differentiation In Members Of The Arisaema Triphyllum (L.) Schott (Araceae) Species Complex, Morgan Bradley Russell

Graduate Theses and Dissertations

The Jack-in-the-pulpit (Arisaema triphyllum (L.) Schott) is an herbaceous perennial herb of North America, and its taxonomic history is full of confusion and rank shifts. Now split into six species, the A. triphyllum species complex consists of morphologically similar taxa that have differing ploidy levels. In Chapter I, I compiled a detailed introduction and history for these taxa, focusing on their taxonomic complexity and morphological ambiguity in herbarium specimens and immature plants. I also presented their ploidy distinctions and how the implications of polyploidy may hold answers to parsing them apart, especially in dried herbarium specimens where defining characteristics are …

Precision To Protein: Comparative Characterization Of Printability, Physicochemical, And Nutritional Properties Of Cricket, Pea, And Whey Proteins In 3d-Printed Nutrition Bars, Sophia Hinkebein

Food Science Undergraduate Honors Theses

Protein is a priority nutrient in the American diet, being fueled by the demands of weight loss drugs, diet culture, and new dietary guidelines. However, meeting these elevated protein goals sustainably requires diversifying beyond standard sources like whey protein isolate. 3D printing offers an opportunity to present a variety of protein sources in a palatable, customized, and consumer-acceptable format. In this study, 3 protein sources (cricket, whey, and pea), were optimized within food inks for 3D printing. Optimization involved different hydration levels, with whey requiring 7 grams of added water, cricket 22 grams, and pea the most at 47 grams. …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Gaining Mussel: Evaluating Reintroduction Success Of Native Freshwater Mussels In Nebraska And Eastern Wyoming, Travis A. Moore

School of Natural Resources: Dissertations, Theses, and Student Research

Freshwater mussels are one of the most imperiled groups of organisms in North America, with over two-thirds of species considered threatened, endangered, or of special concern. These long-lived bivalves are essential to stream ecosystems, acting as filter feeders that improve water quality and as indicators of aquatic health. In Nebraska and Wyoming, native mussel populations have declined sharply due to habitat modification, pollution, and reduced connectivity. Two species of particular concern are the Plain Pocketbook Lampsilis cardium, listed as Tier I at-risk in Nebraska, and the Fatmucket Lampsilis siliquoidea, a Tier II at-risk species. In response, state agencies …

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz

Department of Entomology: Dissertations, Theses, and Student Research

Honeybee health is a complicated issue. Studies were conducted to scale honeybee (Apis mellifera) health from the microbiome to the landscape. Colony health parameters were measured in response to probiotic administration. Colony capped brood production, colony summer weight gain, and Varroa mite incidence were measured. I found that applications of over-the-counter probiotics did not improve the measured parameters of honeybee health compared to a control group. Results suggest that probiotic application was not beneficial but positive benefits may depend on application method, brand, or strain of probiotics used. I also examined how colony health metrics responded to different …

Validation Of A Real-Time Pcr Laboratory Developed Test For The Detection Of Pneumocystis Jirovecii In Bronchoalveolar Lavage Samples, Caitlyn Moon

Honors Scholar Theses

Pneumocystis jirovecii is an opportunistic, host-obligate fungal pathogen responsible for causing P. jirovecii pneumonia (PJP) in humans. PJP is treated with trimethoprim-sulfamethoxazole (TMP-SMX), a strong broad-spectrum antibiotic that carries risk of renal and hepatotoxicity. Therefore, rapid diagnosis or exclusion of PJP is crucial to ensure PJP-positive patients receive effective treatment and PJP-negative patients avoid unnecessary antibiotics. Unlike most microorganisms, P. jirovecii cannot be cultured, presenting complications for the typical methods of diagnosing fungal pathogens. Historically, the gold standard for diagnosis was direct microscopic examination with stains such as methenamine silver and calcofluor white, but this diagnostic technique requires highly skilled …

Phenotypic Contribution And Regulation Of Vibrio Parahaemolyticus Cold-Shock Protein Homologue Vp1889, Oleg Borisovich Ermolinsky

Theses and Dissertations

Vibrio parahaemolyticus is the primary causative agent of a form of gastroenteritis called vibriosis commonly acquired from ingestion of raw and undercooked seafood. The primary mitigation of seafood-borne illnesses is immediate storage on ice following catch, which inhibits bacterial metabolism and replication. Mesophilic bacteria such as V. parahaemolyticus induce expression of cold-shock proteins in a process named the cold shock response, which facilitates survival at low temperatures. V. parahaemolyticus bears 3 homologues of the major Escherichia coli cold-shock protein CspA, an RNA chaperone. Induction of E. coli CspA at low temperatures depends on autogenous modulation by its mRNA structure, which …

The Impact Of Nutrition On Insecticide Resistance And Gene Expression In Aedes Aegypti, Esmeralda Guadalupe Rosas

Theses and Dissertations

The development of insecticide resistance in Aedes aegypti, a major vector of dengue and Zika viruses, threatens vector control efforts, particularly in endemic regions such as South Texas. Insecticides remain widely used; however, their overuse has accelerated metabolic resistance mediated by cytochrome P450 enzymes. Environmental factors such as larval nutrition may influence mosquito physiology and resistance-associated gene expression, yet this relationship remains poorly understood. This study examines how larval nutritional conditions affect the expression of cytochrome P450 genes linked to pyrethroid resistance in Aedes aegypti. Larvae were reared under five dietary treatments differing in lipid and protein ratios, and adults …

Resource Allocation Trade-Offs Between Longevity And Reproduction In Daphnia Magna, Amelia Pearson

Electronic Theses and Dissertations

Although trade-offs between longevity and reproduction have been described for a long time, the genetic mechanisms by which organisms modulate allocation of resources between these two essential processes remain poorly understood, due to their complex nature and their modification by existing nutritional states and environmental cues. Daphnia magna represents a valuable model for investigation in this area due to their moderate lifespan and cyclic parthenogenetic mode of reproduction, allowing for the easy study of gene by environment interactions. This dissertation first investigates the change in mortality, reproduction, activity, and gene expression when fully fed Daphnia and calorically restricted Daphnia are …

Effects Of Weight Loss Medications In Combination With Psychotropic Medications, Emily A. Hoffman

Electronic Theses, Projects, and Dissertations

Background and aims: The obesity epidemic has been well-documented and weight loss medications are becoming increasingly popular. Patients with psychiatric conditions often suffer metabolic side effects from psychotropic medications and are often prescribed weight loss medications to mitigate these side effects. When treating patients on these medications concurrently, understanding their potential side effects is essential for developing an effective care plan. Registered dietitian nutritionists (RDNs), pharmacists, and mental health professionals working in mental health facilities should be aware of these potential side effects to better care for their patients.

Methods: After completing a thorough literature review, a handbook …

Tips For Health Care Practitioners: A Guide For Adolescent Obesity, Regina Santana

Electronic Theses, Projects, and Dissertations

Adolescent obesity continues to be a significant public health concern that is associated with long term physical consequences. The purpose of this project was to develop an evidence based, user friendly guidebook that supports healthcare professionals in the education of adolescents with obesity and their families. A structured literature review development process was used to inform the current development of the guidebook. The guidebook includes components of obesity management such as nutrition and physical activity recommendations. The validity of the content was established through the evaluation of five healthcare professionals that include registered dietitians and licensed therapists. The guidebook was …

Diacylglycerol Kinase Eta As A Novel Target In Nras Mutant Melanoma, Haley P. Wilson, Casey D. Stefanski, Signe Caksa, Glenn L. Mersky, Scott D. Varney, Jelan I. Haj, Dan A. Erkes, Timothy J. Purwin, Vivian Chua, Andrew E. Aplin

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

NRAS mutations occur in 10%-30% of cutaneous melanomas and are associated with high tumor mutational burden. Mutant NRAS signaling drives aberrant cell growth and proliferation, in part, through activation of the RAF-MEK-ERK1/2 kinase pathway; however, targeted therapies to this pathway have limited effectiveness in patients with NRAS mutant melanoma. The role of other targetable signaling pathways in NRAS mutant melanoma is poorly characterized. Here, we demonstrated that one isoform of diacylglycerol kinase, diacylglycerol kinase eta (DGKη), a lipid signaling regulator, was highly expressed in NRAS mutant melanoma patient samples. Knockdown of DGKH in NRAS mutant melanoma cell lines resulted in …

A Tractable Nematode Model For The Emerging Fungal Pathogen, Candida Auris, Melissa Martinez

Dissertations and Theses (Open Access)

Candida auris is an emerging multidrug-resistant opportunistic fungal pathogen. It

poses a significant threat to immunocompromised patients and those with prolonged stays in

healthcare facilities. C. auris spreads rapidly in health care facilities through enhanced

colonization of skin and abiotic surfaces. The genetic factors contributing to this ability and to

the virulence, drug resistance, and stress-tolerant nature of C. auris are largely unknown.

Current animal models of C. auris infections are not well standardized and report conflicting

results. Additional animal models of virulence are needed, especially those amenable to high-

throughput analysis. The nematode Caenorhabditis elegans has been validated as …

Targeting Breast Cancer Brain Metastasis Through Novel Combination Of Fda-Approved Orally Active Blood-Brain Barrier Permeable Ret And Tgli1 Inhibitors, Joshua Cha

Dissertations and Theses (Open Access)

Breast cancer is the most diagnosed cancer in American women, and breast cancer brain metastasis (BCBM) exhibits the worst prognoses with a life-expectancy averaging at 6 months. This short life expectancy is largely attributed to the lack of effective treatment. Many challenges remain unsolved, including the limited number of actionable targets for targeted therapy and effective blood-brain barrier (BBB) permeable drugs that are safe without promoting drug resistance. Therefore, there is an urgent call for identification of novel therapeutic targets and development of BBB-permeable drugs. One of the potential targets identified by our lab for BCBM targeted therapy is the …

Characterization Of Staphylococcus Aureus Clinical Isolates From Deep Musculoskeletal Infections For An In Vitro Biofilm Model For Phage Therapy, Raquel C. Luna

Dissertations and Theses (Open Access)

The rise of multidrug-resistant (MDR) infections is a growing concern in the treatment of deep musculoskeletal infections (MSKIs) and orthopaedic device-related infections (ODRIs). Staphylococcus aureus is responsible for about one third of these infections due to its strong biofilm forming capacity, presence on human skin, and the large number of antibiotic-resistant variants. Bacteriophage (phage) therapy is now being considered as a treatment for MDR MSKIs/ODRIs. We are investigating the use of phage encapsulated into or adsorbed onto biodegradable microspheres for local delivery to the site of these MDR infections. We determined that the anti-staphylococcal phage K effectively lyses the S. …

Refining Treatment Thresholds Of Insect Pests In South Carolina Soybeans Using Simulated Injury And Natural Populations, Adam Whitfield

All Dissertations

Insect pests are a major limiting factor to profitable soybean, Glycine max (L.) Merr., production in South Carolina, yet current economic thresholds are largely based on historical data and may not reflect modern cultivars, production practices, or input costs. Key insect pests in South Carolina can be categorized into three major feeding groups: defoliators, stem feeders, and pod feeders. Defoliating insects include, but are not limited to, soybean looper, Chrysodeixis includens (Walker); velvetbean caterpillar, Anticarsia gemmatalis (Hubner); green cloverworm, Hypena scabra (Fabricius); grasshoppers; and numerous species of beetles. Stem feeders include, but are not limited to, kudzu bug, Megacopta cribraria …

The Impact Of Label Material On Perceived Value And Environmental-Friendliness Of Beverages In Returnable Glass Bottles Versus Actual Environmental Impact Via Life Cycle Assessment, Toni Sharp

All Dissertations

With guidance from the EPA to reduce virgin packaging use and alternatively increase reuse, the concept of reusable packaging has re-emerged as a potential sustainable alternative to single-use and recyclable packaging. For businesses, an understanding of the potential environmental benefits of switching from single-use packaging to reusable packaging, along with consumer willingness to pay for sustainable packaging are paramount. Existing literature on willingness to pay (WTP) for ethical or sustainable products typically relies on consumers’ stated preferences found through surveys using hypothetical products and prices. It has been revealed that sustainability claims printed on the label or products in glass …

Diffuse Radiation Controls On Southern Pine Forest Ecosystem Functioning, Jeremy Forsythe

All Dissertations

Diffuse solar radiation, sunlight scattered by atmospheric aerosols and/or clouds, enhances plant photosynthesis through the diffuse fertilization effect (DFE), increasing ecosystem gross primary productivity (GPP) per unit of incoming radiation. Despite this known effect, pine forests of the southeastern United States remain comparatively understudied in the diffuse light literature. This is a critical knowledge gap, as the southeastern United States sequesters more carbon than any other region of the conterminous country (approximately 176 ± 26 Tg C yr⁻¹, or roughly 56% of national forest net carbon exchange), and the region experiences some of the highest biogenic aerosol loads in North …

Neurobiological Mechanisms Of Attention In Adolescents, Virginia Clark

All Dissertations

Adolescence is a transformative developmental period characterized by profound biological, neurological, and behavioral changes. This dissertation investigates the neurobiological underpinnings of attention in adolescents, emphasizing how internal mechanisms influence and individually shape cognitive development through the endosystem. Rooted in the intersection of human development, learning sciences, and cognitive neuroscience, this work argues for a more integrated understanding of attention as a fundamental cognitive function for learning, one that is behaviorally seen but also biologically and neurologically driven. While developmental science has often emphasized external influences such as family, culture, and environment, this work centers on the adolescent’s internal biological systems …

Developing An Independent Shallow-Water Survey For The Western Rock Lobster Fishery: Tracking Pre-Recruitment Abundance And Habitat Change, Lucy Rudd, Simon De Lestang, Owen Young, Benjamin Hebiton

Fisheries Research Reports

Background

Puerulus settlement has proven to be a good forecaster of legal recruitment of Western Rock Lobster entering into the commercial and recreational fisheries. However, in 2011, this relationship broke down, with average puerulus settlement levels not being reflected in commercial catch rates four years later. Some northern areas showed unexpectedly low catch rates, while other southern areas showed unexpectedly high catch rates. Since this change followed the 2011 marine heatwave, it was assumed that this event changed some component(s) which were important to the growth and survival of juvenile lobsters. It was determined that a better understanding of this …

Application Of Path Analysis To Determine The Impact Of Age And Sex On The Neural Circuits That Promote Nicotine Withdrawal Severity, Veronika Espinoza

Open Access Theses & Dissertations

The rising prevalence of electronic cigarette (e-cigarette) use presents a major public health concern, particularly in adolescent females who are most susceptible to nicotine use and its long-term negative health consequences. The goal of this dissertation was to utilize preclinical rodent models to understand how group differences in age and sex influence neural activation patterns in brain circuits believed to promote nicotine withdrawal. Prior pre-clinical work, conducted largely in male rodents, has established that there are two major brain pathways that modulate the behavioral effects of nicotine withdrawal. These include the mesolimbic and medial habenula-interpeduncular nucleus (MHb-IPN) pathways. To assess …

Prediabetes Prediction Before Disease Onset Using Multimodal Health Data: A Machine Learning Approach, Luisa Veronica Gracia Mazuca

Open Access Theses & Dissertations

Prediabetes is a critical health condition that increases the risk of developing type 2 diabetes. The hemoglobin A1c (HbA1c) test diagnoses patients with prediabetes, but the disease has already caused metabolic alterations. Early detection is essential for timely interventions, and machine learning models offer a promising approach to identify prediabetic individuals through the analysis of biomarkers such as cytokines. We compared four classifiers-logistic regression, decision tree, random forest, and k-nearest neighbors - using cytokines (TNF-α, MCP-1, IL-1β, IL-6, IFN-γ), age, BMI, and waist-to-hip ratio (WHR). Models were evaluated using stratified 5-fold cross-validation and ROC-AUC. K-Nearest Neighbors (k-NN) achieved the highest …

Molecular Mechanisms Of Pfas Induced Protein Dysfunction: Implications For Neurodegenerative Risk, Daisy Lee Wilson

Open Access Theses & Dissertations

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with widespread human exposure and well-established links to adverse health outcomes. However, despite increasing epidemiological and experimental evidence, the molecular mechanisms underlying PFAS-induced neurotoxicity remain incompletely defined. This dissertation investigates PFAS toxicity across biological scales, integrating protein biophysics with neuronal cell models to elucidate pathways relevant to neurodegeneration. At the molecular level, we demonstrate that PFAS disrupts the structure and function of globular proteins, including the impairment of α-lactalbumin's calcium-binding capacity and of β-lactoglobulin's retinol binding, through concentration-dependent conformational destabilization. By applying these findings to a neuronal model, we show that …

Fluoxetine Exposure During Adolescence Alters Prefrontal Cortex Signaling And Nociceptive-Related Behavior In Adulthood, Anapaula Themann

Open Access Theses & Dissertations

The long-term neurobiological consequences of adolescent antidepressant exposure remain poorly understood, despite the substantial increase in selective serotonin reuptake inhibitor (SSRI) prescription rates in youth. Therefore, the goal of this dissertation was to determine whether adolescent exposure to the SSRI fluoxetine (FLX) produces lasting molecular and behavioral adaptations, using rodents as a model system. To do this, in Chapter 2, I assessed whether adolescent FLX exposure (postnatal day [PD] 35-49) alters signaling molecules associated with survival and synaptic plasticity (extracellular signa- regulated kinase [ERK]) within the adult prefrontal cortex (PFC) - a brain region that plays a central role in …

Characterization Of The Antiviral Activity Of Mouse Schlafen Proteins, Wendy Paola Alvarez Leal

Open Access Theses & Dissertations

The goal of this work was to characterize the anti-viral role of mouse Schlafen8 and Schlafen9. Expression of these proteins is induced by type I interferon in response to viral infections. Importantly, their human functional orthologs exhibit anti-viral functions, and the structural element required for these activities are conserved in Schlafen8 and Schlafen9. Therefore, it is expected that these proteins will also impair viral replication. An important limitation in this field is that the anti-viral function of human SLFNs has been evidenced only in in vitro cellular models. No informative mutations have been described in human populations so far. Thus, …

Applying Motivational Interviewing-Based Health Coaching Within An Existing Exercise Referral Program: A Cost, Implementation, And Effectiveness Analysis, Zachary Joseph Friedman

Graduate Theses and Dissertations

Higher levels of physical activity (PA) are known to be associated with lower rates of chronic disease and better quality of living. However, population-wide deficits in PA continue to demand strategies for supporting PA-related behavior change. Health coaching represents a promising intervention strategy for behavior change, but its effectiveness in generally healthy, non-clinical populations is not clear. The purpose of this project was to evaluate the feasibility, cost, and effectiveness of integrating student-delivered, motivational interviewing (MI)-based health coaching into an existing university-based exercise referral program (ERP). Additionally, this study aimed to use students as health coaches, and to document training …

Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser

Biology Graduate Theses

Over the past several decades, cold- and cool-water fish populations across the Upper Midwest, including Minnesota and Wisconsin, have shown troubling signs of decline. Among the most affected is the walleye/Ogaa (Sander vitreus), a species deeply embedded in the ecological balance, cultural identity, and economic vitality of the region, and central to the lifeways, treaty-reserved rights, and resource stewardship of Tribal Nations, including Ojibwe communities across the Upper Midwest. Despite ongoing stocking efforts and harvest regulations, many lake systems have experienced persistent declines in natural recruitment, prompting resource agencies to investigate the underlying causes. One such system is …