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Increased Dairy Product Consumption Is Associated With Shorter Telomere Length In Buccal Cells Among Normotensive Adults, Hsin-Hwa Tsai, Yang-Di Su, Zi-Lun Lai, Chia-Yu Lin, Shu-Fan Lin, Hsiu-Ching Hsu, Wen-Yuan Lin, Po-Ren Hsueh

BioMedicine

Background: Telomere length (TL) is a biomarker of biological aging and a predictor of age-related diseases. Dietary patterns, including dairy consumption, may influence telomere dynamics, but the evidence remains limited, particularly in Asian populations. This study investigates the association between dairy consumption and relative telomere length (RTL) in Taiwanese adults, with results analyzed by hypertension status.

Methods: A cross-sectional survey was conducted among 259 adults in Taipei, Taiwan. RTL was measured using quantitative PCR from buccal cells. Dietary intake was assessed through self-reported questionnaires, with a focus on dairy frequency and fat content. Multiple linear regression models were used to …

Determination Of Cortisol Concentrations Using Different Biomarkers In Dairy Goats During Pregnancy, Fatma Mi̇ra Güven, Erkan Pehli̇van

Turkish Journal of Veterinary & Animal Sciences

Pregnancy is considered one of the most physiologically stressful periods in mammals. In the present study, serum and hair cortisol concentrations were measured in nine female Akkeçi goats, including five primiparous (1.5 years old) and four multiparous (3.5 years old), to evaluate chronic stress during pregnancy. Hair samples were collected in October, November, and December following the introduction of bucks to the herd and within the first 12 h postpartum (corresponding to the 1st, 2nd, 3rd, and 7th sampling times). Hair samples were not collected in January, February, or March (the 4th, 5th, and 6th sampling times) due to insufficient …

Effects Of Zinc-Reinforced Clinoptilolite On Hematological Parameters, Igg Levels, And Enzyme Histochemistry In Fattening Lambs, İhsan Kisadere, Muhi̇tti̇n Zengi̇n, Özkan Demi̇rbaş, Mehmet Faruk Aydin

Turkish Journal of Veterinary & Animal Sciences

The study aimed to determine the effects of zinc-reinforced clinoptilolite supplementation to the diet on selected hematological parameters, serum immunoglobulin G (IgG) concentrations, and enzyme histochemical characteristics in fattening lambs. For this purpose, 40 healthy male lambs were randomly allocated into five equal groups: control (C; n = 8), natural clinoptilolite (NC; n = 8), zinc (ZN; n = 8), natural clinoptilolite plus zinc (NCZN; n = 8), and zinc-reinforced clinoptilolite (ZNRC; n = 8). All animals were housed in individual pens, and the supplementation period continued for 42 days. Blood samples were collected before the trial (day 0) and …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Comparison Of The Mars And Xgboost Algorithms For Predicting Body Weight In Kalahari Red Goats, Louis Thobela Tyasi, Cem Tirink, Kwena Mokoena, Hasan Önder, Uğur Şen, Demet Çanga Boğa, Tolga Tolun, İsmai̇l Gök, Yüksel Aksoy, Lütfi̇ Bayyurt, Ömer Faruk Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Estimating goat body weight from morphometric traits is essential for growth monitoring, health management, and welfare. Accurate weight informs nutrition, clinical interventions, and mating decisions. In this study, the MARS and XGBoost algorithms were used to estimate the live weight of Kalahari Red goats based on body measurements. A dataset containing body length (BL), birth type (single, twin, or triplet), withers height (WH), rump height (RH), and heart girth (HG) information for 200 goats was used in the training and testing of the models. The performances of the models were evaluated with the goodness-of-fit criteria. XGBoost showed a performance of …

Zinc Imbalance And Its Effect On Nutritional Status, Behavior, Gut Microbiota, And Inflammatory Biomarkers In Btbr T+Itpr3tf/J And C57bl/6j Mice: Insights Into Autism Spectrum Disorder, Asma Nasrullah Ahmadani

Thesis/ Dissertation Defenses

Background: Autism spectrum disorder (ASD) has been associated with altered zinc homeostasis and disruptions in the gut microbiota. Zinc is a vital trace mineral involved in multiple biological processes such as inflammatory regulation, intestinal integrity, and neurobehavioral function, and has an important role in influencing microbial activity along the gut–brain axis. Both deficiency and excess of zinc can alter microbial balance. However, their collective effects on behavior, overall physiology, and mineral status in ASD-relevant models are not yet fully understood.

Aim: This comprehensive study explored the effect of dietary zinc variation on body weight, food intake, behavior, gut microbiota composition, …

Electric Pressure Cookers: What You Need To Know, Callahan Adams, Emily Smith

All Current Publications

Electric pressure cookers (EPCs) offer convenience, versatility, and efficiency in everyday cooking. EPCs offer multiple cooking functions beyond pressure cooking, including sautéing, slow cooking, steaming, cooking rice, and preparing yogurt. They are not suitable for pressure canning, as confirmed by multiple Extension research studies and national guidelines. When used properly, EPCs provide a safe and effective way to prepare meals quickly while saving counter and storage space.

The Effect Of Vitamin D Supplementation On Serum 25(Oh)D And Its Association With Stress Fractures In High-Risk, Physically Active Adults: A Critically Appraised Topic, Connor Lewis, Martha Yost, Aric J. Warren

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Clinical Scenario: In collegiate athletic and military populations alike, the repetitive and intense training increase the risk of experiencing a stress fracture, representing a significant time loss and financial cost. Research suggests the use of supplemental vitamin D to prevent the occurrence of stress fractures in high-risk adults. Clinical Question: In high risk, physically active adults, does the effect of vitamin D supplementation on serum 25-hydroxyvitamin D [25(OH)D] concentration effect the occurrence of stress fractures? Clinical Bottom Line: The findings suggest that clinicians should consider the use of supplemental vitamin D as a protective measure against the occurrence of stress …

Role Of Developmental Plasticity In Tethered Flight Performance Of The Oriental Fruit Fly, Bactrocera Dorsalis (Hendel) (Diptera: Tephritidae), Thabang P. Moropa, Steve B.S. Baleba, Christopher W. Weldon

All Peer-Reviewed Publications

This study investigated the effect of nutrition on development of wings and adult body condition of Bactrocera dorsalis (Hendel), an exotic pest of Asian origin and a species of economic importance in South Africa. Six host fruit types were used to rear B. dorsalis: mango, guava, orange, grapefruit, papaya and apple. One day after emergence, adults were used for tethered flight assessments using computerised flight mills. Additional flies were assessed in terms of wing morphology using geometric morphometrics, flight muscle mass, and lipid content. There were significant differences in the larval and pupal developmental time as well as larval and …

Selenium-Thioredoxin Axis Contributes To Ferroptosis Resistance In Pancreatic Cancer Cells, Arslan Amer, Micah Olamide Idowu, Aqsa Ahsan, Alyssa A. Abbas, Tahiyat Alothaim, Xiaohu Tang

Michigan Tech Publications

Pancreatic ductal adenocarcinoma (PDAC) shows substantial heterogeneity in cysteine dependence and ferroptosis sensitivity. We identify two PDAC subtypes distinguished by EMT status: mesenchymal-like cells are highly cysteine-dependent and rapidly undergo ferroptosis upon cystine deprivation or system xc⁻ inhibition, whereas epithelial-type cells are ferroptosis-resistant. Selenium supplementation protects cells from erastin-induced ferroptosis, and this protection persists even when intracellular glutathione (GSH) is depleted, supporting an additional GPX4-independent protective mechanism. Sepp1 knockdown does not alter sensitivity, indicating that selenium’s protective effect is independent of Sepp1. Instead, epithelial-type cells rely on both cytosolic and mitochondrial thioredoxin reductases (TrxR1 and TrxR2) to maintain ferroptosis …

Effect Of Feeding Levels With High Micronutrients On The Reproductive Performance And Plasma Progesterone Concentration In Early Gestation Sows, Kewalin Kongngern, Liang-Chou Hsia, Shu-Ping Su, Wantamas Jantasin, Jai-Wei Lee

The Thai Journal of Veterinary Medicine

This experiment investigated the effect of feeding levels with high micronutrients on the reproductive performance and plasma progesterone concentration in early gestation sows. Thirty-six first-parity sows (Landrace × Yorkshire × Duroc), with an initial average body weight of 160.85 kg, were randomly allocated to four dietary treatments during early gestation: Treatment 1) fed 1.5 kg/day (days 1-30), Treatment 2) fed 1.5 kg/day with 1.7 times the vitamins and minerals (total vitamin E 150 IU) (days 1-15) and fed 1.5 kg/day (days 16-30), Treatment 3) fed 1.5 kg/day with 1.7 times the vitamins and minerals (total vitamin E 150 IU) (days …

From Waste To Sustainability: Exploring Consumer Purchase Intentions Of Milk Enriched With Upcycled Peanut Skins, Peng Lu, Fan Xu, Jennifer Waldeck, Keith Weber, Hualu Zhou

Communication Faculty Articles and Research

Highlights

• National survey investigated consumer acceptance of milk enriched with upcycled peanut skins.
• Developed a conceptual model based on Technology Acceptance Model.
• Identified that consumer attitudes and emotions are essential in the purchase intentions of upcycled foods.
• Identified social influence as a determinator of consumers' attitudes and purchase intentions.
• Inform and advance research, practice, and policy that promotes upcycled food products.

Gender Differences In College Students’ Physical Activity Self-Efficacy, Social Comparison Beliefs, And External Frames Of Reference, Ken Murfay, Sarah Pyszczynski, Sarah Collins

International Journal of Physical Activity and Health

This study examined whom college-aged individuals believe observing would more likely increase their physical activity self-efficacy (PASE) for a physical skill, whom they are more likely to compare their abilities to within a physical education (PE) class, how often they compare themselves to others in PE, and what students’ PASE scores were. Based on the mean scores for each question, students were more likely to compare themselves to a student than a teacher, friend than a non-friend, and similar or higher skilled peer than a lower skilled peer. To increase students’ confidence for a physical skill, students had higher levels …

Conditioned Feed Aversion As A Means Of Conditioning Livestock To Avoid Consuming Toxic Plants Growing In South Africa : An Overview, Leendert D. Snyman, Frederick D. Provenza

Poisonous Plant Research (PPR)

Significant economic losses to the livestock industry are caused by the ingestion of toxic plants in South Africa, among which the most dangerous ones are Moraea pallida (geeltulp), Urginea/Drimea sanguinea (rooislangkop), Senecio latifolius (Staggers Ragwort), and Geigeria ornativa (vermeerbos). Conditioned feed aversion is a potential means to address this issue. Treating animals with an aversive substance along with an extract of the sensory characteristics of a specific plant conditions animals to avoid the plant. Geigeria ornativa, unlike the other three plants, is non-aversive. Following treatment for aversion, the induced aversion fades with time and eventually breaks down. Continuous exposure …

A Case Study Of Suspected Sheep Losses Due To Chokecherry Not Death Camas, Kevin D. Welch, Clint A. Stonecipher, Stephen T. Lee, Daniel Cook, Benedict T. Green

Poisonous Plant Research (PPR)

Death camas (Zigadenus paniculatus) is a toxic plant often associated with poisoning cases in livestock, especially in sheep, in the spring on mountain foothills. Consequently, when sheep are found dead in mountain foothills, and death camas is present, it is often implicated as the potential cause of death. However, this may be over simplistic as there may be other plants that may also contribute to, or are the cause of, death in sheep. In this study, we present a case of approximately 200 sheep out of a band of 400 that died within 24 hours of beginning to …

Sex-Specific Differences In Mediobasal Hypothalamus In Response To Nutritional States, Jonathan C Bean, Jinjing Jian, Tzu-Chiao Lu, Hailan Liu, Kristine Conde, Darah A Threat, Sanika V Jossy, Megan E Burt, Jingjing Cheng, Yue Deng, Xing Fang, Xiaoyu Geng, Junying Han, Yongxiang Li, Hesong Liu, Qingzhuo Liu, Yutian Liu, Yuhan Shi, Longlong Tu, Mengjie Wang, Xu Xu, Yuxue Yang, Meng Yu, Xinming Liu, Meixin Sun, Fuhui Wang, Olivia Z Ginnard, Yongjie Yang, Yang He, Chunmei Wang, Yanyan Qi, Hongjie Li, Yong Xu

Children’s Nutrition Research Center Staff Publications

The arcuate nucleus of the hypothalamus plays a central role in sensing and integrating nutritional, hormonal, and neural signals that regulate feeding, energy homeostasis, growth, and reproduction, all of which show pronounced sex differences. However, the cellular mechanisms underlying these responses remain poorly understood. We performed snRNA-seq of the mediobasal hypothalamus, focusing on the arcuate nucleus, in female and male mice under different nutritional states. Analysis of 42 cell types revealed that Agrp neurons were most sensitive to nutritional changes, dopaminergic neurons showed strong sex-specific differences, and KNDy neurons were highly responsive to both sex and nutrition. Pomc neurons displayed …

Determinasi Minimum Dietary Diversity Pada Anak Usia 6-23 Bulan: Analisis Survei Kesehatan Indonesia 2023, Elfys Ferdynan, Besral Besral

Jurnal Biostatistik, Kependudukan, dan Informatika Kesehatan

Keragaman pangan minimum atau minimum dietary diversity (MDD) merupakan indikator penting kualitas konsumsi pangan anak usia 6-23 bulan. Pemenuhan MDD berperan dalam mendukung pertumbuhan optimal dan pencegahan malnutrisi, termasuk stunting. Penelitian ini bertujuan untuk menganalisis faktor-faktor yang berhubungan dengan pemenuhan MDD pada anak usia 6-23 bulan di Indonesia. Data yang digunakan adalah data Survei Kesehatan Indonesia 2023 dengan desain potong lintang. Subjek penelitian adalah 17.991 anak usia 6-23 bulan. Analisis yang dilakukan menggunakan regresi logistik biner dengan penyesuaian desain complex samples. Hasil penelitian menunjukkan bahwa faktor yang meningkatkan peluang pemenuhan MDD secara signifikan meliputi usia anak 12-23 bulan, …

Food Councils And Federal Committees: Community Responses To Inaction On Food Insecurity By Government, Kevin O'Connell, Liam R. Rupprecht

Binghamton University Undergraduate Journal

Food insecurity is a devastating force in the United States, as shown by its destructive impact on vulnerable groups like children, racial minorities, and those in poverty. Non-white urban communities of color especially have significantly higher rates of household food insecurity due to systemic racism in American food systems. Despite the prevalence of these factors, they are consistently overlooked by public and private forces. Federal efforts have failed to resolve the issue, leaving it to local “food council” organizations that attempt to help through advocacy and charity. These groups face a difficult task: how can they work to mitigate food …

Utah's Value-Added Food Processing Options, Karin E. Allen, Kynda R. Curtis, Makaylie Langford

All Current Publications

This fact sheet provides an overview of the benefits of producing and marketing value-added foods and outlines key considerations for new ventures. It also details the two primary programs available to smaller-scale processing in Utah—the Cottage Food Program and the Homemade Food Act—assisting producers in evaluating their products, markets, and resources to choose the best path forward.

International Society Of Sports Nutrition Position Stand: Effects Of Dietary Antioxidants On Exercise And Sports Performance, Drew E. Gonzalez, Broderick L. Dickerson, Brandon M. Roberts, Jennifer A. Kurtz, Adam M. Gonzalez, Matthew J. Mcallister, Jeffrey L. Heileson, Richard J. Bloomer, Shawn M. Arent, Darren G. Candow, Jeffrey R. Stout, Karen A. Hecht, Bill Campbell, Chad M. Kerksick, Douglas Kalman, Jose Antoino, Richard B. Kreider

Faculty Publications

Following a comprehensive review, the International Society of Sports Nutrition (ISSN) has developed an official position on the role of dietary antioxidants in exercise and sport. Antioxidants play a complex, context-dependent role in vivo; they can facilitate recovery from exercise but may also hinder training adaptations when consumed at supraphysiological doses. While endogenous antioxidant systems can effectively maintain redox balance, dietary sources, particularly whole foods, can help mitigate excessive oxidative stress following intense/heavy training or inadequate recovery. The influence of dietary antioxidants depend on timing, dosage, type, and individual factors. The ISSN’s official position encompasses the following: (1) Redox balance …

Molecular Detection Of Genes Encoded For Biofilm Formation In Bacteria Isolated From The Oral Cavity Of Patients, Tebarek Al-Taei, Ali A. Al-Hisnawi

Karbala International Journal of Modern Science

Background: A biofilm is a collection of the microbial cells attached to the surface that are encased in a matrix of extracellular polymers. Biofilm formation creates a physical and chemical barrier that prevents antibiotics from reaching bacterial cells, resulting in treatment failure and reinfection. The present study aimed to investigate the biofilm formation in bacteria isolated from the oral cavity of patients.

Methods: In this study, 150 bacterial isolates were obtained from tooth and gum surface swabs of male and female patients of different ages who visited specialized centers and dental clinics in Babylon City after they were clinically diagnosed …

Applied Research For Corn Production In Indiana, 2022, Daniel Quinn

Purdue University Press Books

The 2022 edition of Applied Research for Corn Production in Indiana offers accessible, research-based tips for farmers who want to improve corn production practices by using applied field-research trials. Daniel Quinn and the Purdue University Corn Agronomy team reveal practical ways to boost yields, reduce costs, and improve sustainability on the farm. Their findings are derived from applied research trials across the state and relate to important topics such as planting practices, fertilizer use, crop nutrition, cover crops, and new technologies. Applied Research for Corn Production in Indiana distills complex research into clear insights and supplies corn producers with effective …

Charting A New Era To Modernize United States Infant Formula Regulation, Douglas G Burrin, Amy B Hair, Sharon M Donovan

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Metagenomic Insights Into Antimicrobial Resistance In Small-Scale Poultry And Cattle Farms, Elijah Ayilaran, Agnes Kilonzo-Nthenge Agnes Kilonzo-Nthenge

Food and Animal Sciences Faculty Research

Antimicrobial resistance (AMR) poses a critical challenge to global health, with food animal production systems recognized as significant reservoirs of antimicrobial-resistant bacteria. This study evaluated the prevalence and distribution of antimicrobial resistance genes (ARGs) and virulence factors (VFs) across small-scale poultry and cattle farms. A total of 468 samples (soil, feces, water, and natural land soil) were collected from four farms and analyzed using shotgun metagenomics. Proteobacteria (34.91%) were the dominant phylum across environments, followed by Cyanobacteria (15.67%), Actinobacteria (14.95%), Firmicutes (10.57%), and Bacteroidetes (8.69%). Tetracycline (33.41%) and beta-lactam (30.30%) resistance genes were the most abundant, with macrolide (9.32%) and …

Tumor-Immune-Neural Circuit Disrupts Energy Homeostasis In Cancer Cachexia, Xiuhui Shi, Alex X Arreola, Zhijun Zhou, Jingxuan Yang, Mingyang Liu, Yang Cai, Yu Ren, Hao Yuan, Qun Chen, Xinjie Chen, Xinyu Yang, Yimei Meng, Jingyi Wang, Wenyi Luo, Michael C Rudolph, Rohan Varshney, Kar-Ming Fung, Chao Xu, Wei R Chen, Michael S Bronze, Lei Zheng, Yi-Ping Li, Courtney W Houchen, Yuqing Zhang, Min Li

Faculty, Staff and Student Publications

Cancer-induced cachexia and anorexia are debilitating complications across many cancers, yet effective treatments remain limited due to a poor understanding of the underlying mechanisms. Here, we identify an uncharacterized tumor-immune-neural circuit driving these syndromes, centered on growth and differentiation factor 15 (GDF15). Using genetically engineered mouse models, we find that loss of GDF15 protects against appetite loss, muscle wasting, and fat loss in pancreatic, lung, and skin cancers. Single-cell RNA sequencing reveals macrophages as a major source of GDF15, induced by tumor-derived colony-stimulating factor 1 (CSF1). GDF15 acts via the central nervous system to enhance β-adrenergic signaling in the tumor …

Community Participatory Co-Design And Development Of A Digital Diabetes Prevention Education Program For Hispanic Families With Obesity: Mixed Methods Study, Sandra Mihail, Marbelly Partida, Lizette Villanueva, Debbe Thompson, Teresia M O'Connor, Salma M Musaad, Maria J Redondo, Erica G Soltero

Children’s Nutrition Research Center Staff Publications

Background: Digital health interventions (DHIs) can extend the reach of disease prevention interventions; however, few are evidence-based, theoretically grounded, or developed for high-risk youth and families. Co-design approaches engage end users in the design and development of the DHI, which can lead to increased accessibility and engagement.

Objective: This study aimed to describe the adaptation of an evidence-based diabetes prevention program for remote, digital delivery.

Methods: The adaptation of the in-person intervention was guided by a modified Inclusive Digital Health Intervention Design to Promote Health Equity framework and conducted in collaboration with Hispanic adolescents (n=23) with obesity (BMI ≥95th percentile) …

A Framework For Assessing The Trustworthiness Of Scientific Research Findings, Brian A Nosek, David B Allison, Kathleen Hall Jamieson, Marcia Mcnutt, A Beau Nielsen, Susan M Wolf

Children’s Nutrition Research Center Staff Publications

Vigorous debate has erupted over the trustworthiness of scientific research findings in a number of domains. The question "what makes research findings trustworthy?" elicits different answers depending on whether the emphasis is on research integrity and ethics, research methods, transparency, inclusion, assessment and peer review, or scholarly communication. Each provides partial insight. We offer a systems approach that focuses on whether the research is accountable, evaluable, well-formulated, has been evaluated, controls for bias, reduces error, and whether the claims are warranted by the evidence. We tie each of these components to measurable indicators of trustworthiness for evaluating the research itself, …

Performance Of An Automated Sleep Scoring Approach For Actigraphy Data In Children And Adolescents, Pin-Wei Chen, Erica C Jansen, Christopher M Cielo, Ariel A Williamson, Margaret Banker, Michael Kaye, Peter X K Song, Karen E Peterson, Alejandra Cantoral, Martha María Téllez-Rojo, Cathy Goldstein, Khadija Zanna, Akane Sano, Jennette P Moreno, Heidi Kalkwarf, Babette S Zemel, Jonathan A Mitchell

Children’s Nutrition Research Center Staff Publications

Study objectives: GGIR is an R package for processing raw acceleration data to estimate sleep health parameters. We aimed to (1) assess the performance of three sleep algorithms within GGIR against PSG for detecting sleep/wake in clinically referred, typically-developing children (criterion validity); and (2) describe GGIR-derived sleep estimates from typically developing children enrolled in multiple cohort studies (face validity).

Methods: For criterion evaluation, children (8-16 years, N = 30) wore an actigraphy device for one night during in-lab polysomnography with performance assessed using epoch-by-epoch analyses. For face validity evaluation, four community/free living datasets were used: (1) Bone Mineral Accretion in …

Discovery Of Horsfieldia Macrothyrsa Bioactives With Cytotoxic Effects On Breast Cancer Cells Through In Vitro And Docking Analyses, Megawati Megawati, Akhmad Darmawan, Agus Budiawan Naro Putra, Kartika Dyah Palupi, Marissa Angelina, Ahmad Randy, Siska Andrina Kusumastuti, Faris Hermawan, Sumi Hudiyono

Karbala International Journal of Modern Science

This study investigated the phytochemical constituents and biological activities of Horsfieldia macrothyrsa leaves to identify their bioactive compounds. Methanol extracts were fractionated using n-hexane and ethyl acetate, followed by silica gel column chromatography with a stepwise polarity gradient. From 100 g of powdered leaves, three major    compounds were successfully isolated from the ethyl acetate fraction: 1-(2,4,6-trihydroxyphenyl)dodecan-1-one (1), sesamin (2), and β-sitosterol (3). Their chemical structures were confirmed using UV, IR, LC–MS/MS, and NMR  spectroscopy. Biological activities of the fractions and isolates were evaluated through DPPH antioxidant assays, α-glucosidase inhibition for antidiabetic activity, MTT …

Impaired Arginine, Citrulline And Glutamine Metabolism In Type 2 Diabetes: Insights From A Stable Isotope Study, Mustafa Tosur, Raven A Wierzchowska-Mcnew, Nicolaas E P Deutz, Mariëlle P K J Engelen

Children’s Nutrition Research Center Staff Publications

Context: Arginine (Arg) is an important amino acid in T2D as a potent insulin secretagogue and precursor for nitric oxide (NO). Citrulline (Cit), the substrate for de novo Arg synthesis, is mostly produced from glutamine (Gln).

Objective: We aimed to investigate their metabolism in T2D using novel stable isotope tracer approach.

Methods: We studied 42 individuals (21 with T2D, 21 controls). After overnight fasting, blood samples were collected following pulse administration of stable amino acid tracers. Plasma concentrations and isotopic enrichments were measured by LC-MS/MS, and compartmental analyses were performed to calculate their whole-body production (WBP) rates and kinetics.

Results: …