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Effect Of Chemical Enhancers On Transdermal Delivery Of Amiloride For Management Of Resistant Hypertension, Oluwatosin Tabitha Leshaodo

Electronic Theses and Dissertations

Amiloride, a diuretic used for managing resistant hypertension, is hindered by low oral bioavailability and Gastrointestinal side effects, impacting patient compliance. This study investigates transdermal delivery as an alternative to reduce pill burden and improve adherence. Using dermatomed porcine ear skin, the research examined the passive transdermal delivery of amiloride and the effect of various chemical enhancers, including oleic acid, oleyl alcohol, tocopheryl polyethylene glycol succinate, and N-methyl-2-pyrrolidone, individually or in combination. A validated High-Performance Liquid Chromatography method, adhering to Internal Council for Harmonized guidelines (ICH), was employed for quantitative analysis. In vitro permeation studies, conducted using porcine skin and …

Abstracts Of The 12th Annual Mississippi Association Of Entomologists, Nematologists, And Plant Pathologists Meeting, October 30 And 31, 2023, Mississippi Association Of Entomologists, Nematologists, And Plant Pathologists

Midsouth Entomologist

No abstract provided.

Why Do Patients With Cancer Die?, Adrienne Boire, Katy Burke, Thomas R Cox, Theresa Guise, Mariam Jamal-Hanjani, Tobias Janowitz, Rosandra Kaplan, Rebecca Lee, Charles Swanton, Matthew G Vander Heiden, Erik Sahai

Faculty, Staff and Student Publications

Cancer is a major cause of global mortality, both in affluent countries and increasingly in developing nations. Many patients with cancer experience reduced life expectancy and have metastatic disease at the time of death. However, the more precise causes of mortality and patient deterioration before death remain poorly understood. This scarcity of information, particularly the lack of mechanistic insights, presents a challenge for the development of novel treatment strategies to improve the quality of, and potentially extend, life for patients with late-stage cancer. In addition, earlier deployment of existing strategies to prolong quality of life is highly desirable. In this …

Markov Models For Clinical Decision-Making In Radiation Oncology: A Systematic Review, Lucas B Mccullum, Aysenur Karagoz, Cem Dede, Raul Garcia, Fatemeh Nosrat, Mehdi Hemmati, Seyedmohammadhossein Hosseinian, Andrew J Schaefer, Clifton D Fuller

Faculty, Staff and Student Publications

The intrinsic stochasticity of patients' response to treatment is a major consideration for clinical decision-making in radiation therapy. Markov models are powerful tools to capture this stochasticity and render effective treatment decisions. This paper provides an overview of the Markov models for clinical decision analysis in radiation oncology. A comprehensive literature search was conducted within MEDLINE using PubMed, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Only studies published from 2000 to 2023 were considered. Selected publications were summarized in two categories: (i) studies that compare two (or more) fixed treatment policies using Monte Carlo simulation …

Why Do Patients With Cancer Die?, Adrienne Boire, Katy Burke, Thomas R Cox, Theresa Guise, Mariam Jamal-Hanjani, Tobias Janowitz, Rosandra Kaplan, Rebecca Lee, Charles Swanton, Matthew G Vander Heiden, Erik Sahai

Faculty, Staff and Student Publications

Cancer is a major cause of global mortality, both in affluent countries and increasingly in developing nations. Many patients with cancer experience reduced life expectancy and have metastatic disease at the time of death. However, the more precise causes of mortality and patient deterioration before death remain poorly understood. This scarcity of information, particularly the lack of mechanistic insights, presents a challenge for the development of novel treatment strategies to improve the quality of, and potentially extend, life for patients with late-stage cancer. In addition, earlier deployment of existing strategies to prolong quality of life is highly desirable. In this …

Canine Parasites In Southern Nevada Urban Dog Parks: Paravec Study, Miklo Azrael A. Alcala

UNLV Theses, Dissertations, Professional Papers, and Capstones

While dogs are one of humans’ greatest companions, they also serve as hosts for a multitude of parasites. Urban dog parks serve as environments where the transmission of diseases is likely to occur. Exposure to infective agents, contained within the soil, intermediate hosts (e.g., rodents, rabbits), and feces, can directly affect the health of dogs and their owners. Other factors that can further influence canine health include age, previous diagnoses of parasites/diseases, and migration. Many dog owners visit dog parks to encourage socialization and exercise. Such activities are meant to improve health, but unawareness of microbiological organisms can lead to …

Variability In Performance Of Genetic-Enhanced Dxa-Bmd Prediction Models Across Diverse Ethnic And Geographic Populations: A Risk Prediction Study, Yong Liu, Xiang-He Meng, Chong Wu, Kuan-Jui Su, Anqi Liu, Qing Tian, Lan-Juan Zhao, Chuan Qiu, Zhe Luo, Martha I Gonzalez-Ramirez, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng

Faculty, Staff and Student Publications

BACKGROUND: Osteoporosis is a major global health issue, weakening bones and increasing fracture risk. Dual-energy X-ray absorptiometry (DXA) is the standard for measuring bone mineral density (BMD) and diagnosing osteoporosis, but its costliness and complexity impede widespread screening adoption. Predictive modeling using genetic and clinical data offers a cost-effective alternative for assessing osteoporosis and fracture risk. This study aims to develop BMD prediction models using data from the UK Biobank (UKBB) and test their performance across different ethnic and geographical populations.

METHODS AND FINDINGS: We developed BMD prediction models for the femoral neck (FNK) and lumbar spine (SPN) using both …

Analysis Of Malarial Impact: Understanding Disease Transmission, Epidemiology, And Effects On Fetal Life, Josey Sumpter

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Malaria is a significant public health problem which mainly affects malaria endemic regions, such as parts of Africa, South America, and Western Asia. However, all hot and humid environments are susceptible to spread leading to a global scale. Malaria affects an estimated 200 million people each year, with about 600,000 cases resulting in death. Malaria is passed through the bites of infected female anopheles mosquitos, with one of the six Plasmodium genus parasites that are malarial causing. Once the parasite enters the blood stream it infects erythrocytes which can then spread throughout the entire body. Disease is often characterized by …

Development Of A Flow Cytometry Method For The Identification And Enumeration Of Enterococcus Faecalis, Riza Magbitang

Master's Theses

A multi-color flow cytometry method coupled with fluorescent staining was developed to detect and quantify E. faecalis concentrations in coastal waters as an alternative to the traditional membrane filtration method. The developed method was used to characterize E. faecalis during batch growth and yielded consistent results across the various physiological states of the cells. Additionally, the application of propidium iodide (i.e., a viability stain), afforded the method the ability to differentiate between cells with intact and compromised cell membranes, which raises the potential of quantifying cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked …

Development And Validation Of Aronia Melanocarpa Berry Recipes For Home Canning: Integrating Thermal Lethality Studies, Microbiological Safety, And Antioxidant Analysis, Juan Diego Villegas Posada

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Aronia melanocarpa, also known as chokeberry, is gaining popularity for its high antioxidant content and health benefits, including reducing oxidative stress and inhibiting cancer gene expression. However, its characteristic bitterness and astringency limit its broader appeal. Chokeberry's low pH places it in the acid foods category, making it ideal for safe home canning. An acidic environment combined with heat treatment inhibits harmful bacteria like Clostridium botulinum. Home canning preserves food by sealing it in airtight containers and heating it to destroy spoilage microorganisms.

Developing safe and palatable recipes is essential for making chokeberries suitable for home canning. A …

Preschool Executive Control And Adolescent Dietary Outcomes: Highlighting The Role Of Context, Marla Lopez

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Research has identified executive control (EC) as an important predictor of dietary outcomes among youth. Executive control in preschool age has been shown to predict important outcomes across development for youth, indicating that good, foundational EC abilities are associated with successful development in later life. Dietary outcomes across adolescence, an important transitional period for establishing healthy eating behaviors, are understudied. Therefore, the current study sought to link two critical developmental periods, examining how preschool EC is associated with dietary trajectories across adolescence. Further, the study aimed to identify how contextual factors, specifically indices of socio-economic status and the home and …

Development Of An Egg-Specific Mass Spectrometry Targeted Method For Processed Food Matrices, Liyun Zhang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Egg is a major food allergen in the United States, causing symptoms from mild reactions to severe anaphylaxis. An egg-free diet remains the only management approach for egg allergy. Therefore, individuals and families living with egg allergies rely heavily on allergen statements on packaged food products for informed food choices. Currently, no labeling regulations exist for unintentionally introduced allergens, posing risks to consumers with egg allergies. Therefore, it is critical to have an accurate and reliable detection method in allergen control and management to determine the concentration of total protein from allergenic food sources. Traditional enzyme-linked immunosorbent assays are routinely …

The Benefits Of Biofouling – Promoting The Growth Of Benthic Organisms To Enhance Ecosystem Services, Kailey Nicole Richard, Kelli Z. Hunsucker, Travis J. Hunsucker, Geoffrey Swain

Ocean Engineering and Marine Sciences Student Publications

For the marine industry biofouling has a negative reputation. On ship hulls, the accumulation of these unwanted plants and animals can lead to increased drag, fuel consumption, and greenhouse gas emissions. Offshore platforms are also subject to biofouling which can result in corrosion and hydrodynamic loading, thus shortening their lifespan. While the harmful impacts of biofouling are commonly reported throughout the literature, biofouling can also benefit both aquatic and human populations. Common biofouling organisms act as natural filtration systems, thus improving water quality. Many of the same flora and fauna serve as a food source, and structures could be designed …

Microbiome Assembly And Function In The Solitary Mason Bee, Osmia Lignaria (Megachilidae), Bailey Crowley

All Graduate Theses and Dissertations, Fall 2023 to Present

Animal-microbe interactions can influence host biology, ecology, and evolution. The assembly and function of microbes found within animal hosts oftentimes depends on which species are involved. Advances in sequencing technologies have permitted the exploration of host-microbe interactions in a variety of animals, including bees. Early research aimed at understanding the microbiomes of social bees, such as honey bees and bumble bees, found that microbes prevent the spoilage of stored pollen, breakdown indigestible nutrients into smaller molecules available for uptake by the host, and also protect the host from pathogens. When environmental stressors, such as increased temperatures, disrupt the microbiome, the …

Value Of Macroalgae As Habitat In A Eutrophic Coastal Bay, Dominika Polchowska

Theses, Dissertations and Culminating Projects

Eutrophication is a phenomenon caused by changes in nutrient loading that results in harmful algal blooms, an overgrowth of macroalgae, altered aquatic benthic invertebrate communities, and loss of ecosystem functions. Barnegat Bay, NJ is a highly eutrophic system that has experienced a substantial loss of seagrass beds with subsequent expansion of drift macroalgae. Although considered poor quality, these algal beds represent valuable habitat for many benthic invertebrates. This research assesses the value of macroalgae as a habitat for macrofauna in regions where macroalgae are the dominant “vegetation.” Benthic drift algal samples were collected during June and August 2023 to reflect …

Atypical Responses To Sensory Stimuli Or Eating Environments In Children With Autism Spectrum Disorder, Lydia Rockers

Graduate Theses and Dissertations

Children with autism spectrum disorder (ASD) often encounter challenges in processing sensory inputs. Children with ASD may experience feeding difficulties and over- or under- stimulation during food-related activities such as eating or shopping. These have impacts on the health and well-being of individuals with ASD in childhood and throughout their lives. Further research is needed to address the impact of ambient sound levels in a retail or restaurant setting. The main objective of this thesis was to address these gaps by identifying responses to various environmental and food related stimuli. The findings of this research addressed differences in responses between …

The Effect Of Fat Content On The Inactivation And Recovery Of Listeria Spp. In Ready-To-Eat Foods After High Pressure Processing, Yhuliana Kattalina Niño Fuerte

Department of Food Science and Technology: Dissertations, Theses, and Student Research

High-pressure processing (HPP) is recognized by the United States Department of Agriculture Food Safety and Inspection Service (USDA FSIS) as a post-lethality treatment to control Listeria monocytogenes in ready-to-eat (RTE) meat and poultry products. A scoping review of 603 journal articles, which ultimately was narrowed down to 16 articles, revealed that the efficacy of HPP varies with fat content. The studies demonstrated that L. monocytogenes inactivation by HPP tends to be lower in RTE meats with greater than 5% fat content, compared to products with lower than 5% fat. The wide range of HPP parameters used in these studies …

Survey Of Nematodes Associated With Strawberry In South Carolina And Evaluation Of Short-Term Heat Exposure On Nematode Reproduction Biology, Ivan A. Alarcon Mendoza

All Theses

Nematodes are a growing threat to the multi-billion-dollar strawberry industry in the US due to the lack of safe and effective management methods. Limited information on the distribution of nematodes in fumigated plasticulture strawberry fields further complicates their management. Additionally, climatic anomalies like rising soil temperatures and heat waves are occurring more often, and their impact on crop growth and nematode reproduction is not well understood. The current study was conducted with two main objectives: i) understand distribution and population densities of nematodes associated with plasticulture strawberry production in South Carolina, and ii) understand the impact of short-term high heat …

Effects Of Interrupting The Scotophase Period On Bone Health And Performance Of Laying Hens, Alexis J. Clark

All Theses

This thesis aimed to evaluate the effects of interrupting the daily scotophase period on bone health and production in laying hens. Modern commercial laying hens are prone to high rates of bone demineralization due to the constant mobilization of calcium to withstand daily eggshell calcification mechanisms. The industry light standard for laying hens is 16 hours of continuous light (photophase) and 8 hours of continuous darkness (scotophase) daily. During scotophase, physical activity is reduced due to instinctive perching behaviors, and thus, feeding behaviors are halted. However, physiological processes, including digestion, excretion, and reproduction, remain active during scotophase.

Most eggshell formation …

Thiol Dough Conditioners Impact On The Formation Of Advanced Glycation End-Products In Bagels Containing Sunflower Seed Flour, Alton Chambers

Food Science (MS) Theses

Baking bagels can increase advanced glycation end products (AGEs), which are linked to increased metabolic and chronic diseases. This research correlated color and the proximate composition with fluorescent AGEs in bagels containing 0-20% sunflower flour (SFF) and 0-90 ppm L-cysteine (Cys) or Glutathione (GSH). No significant differences were observed in the moisture and lipid content for the bagel crust and crumb. Carbohydrate content decreased, while protein and ash content significantly increased when the SFF increased from 0 to 20 % SFF. The pH of the crust and crumb increased from 5.27 to 6.01 and 5.33 to 6.09, respectively, when sunflower …

Bacterial Chondronecrosis With Osteomyelitis Lameness In Broiler Chickens And Its Implications For Welfare, Meat Safety, And Quality: A Review, Amanda Anthney, Anh Dang Trieu Do, Adnan A.K. Alrubaye

Poultry Science Faculty Publications and Presentations

The exponential increase in global population continues to present an ongoing challenge for livestock producers worldwide to consistently provide a safe, high-quality, and affordable source of protein for consumers. In the last 50 years, the poultry industry has spearheaded this effort thanks to focused genetic and genomic selection for feed-efficient, high-yielding broilers. However, such intense selection for productive traits, along with conventional industry farming practices, has also presented the industry with a myriad of serious issues that negatively impacted animal health, welfare, and productivity–such as woody breast and virulent diseases commonly associated with poultry farming. Bacterial chondronecrosis with osteomyelitis (BCO) …

Assessing The Reproducibility Of Research Based On The Food And Drug Administration Manufacturer And User Facility Device Experience Data, Xinyu Li, Yubo Feng, Yang Gong, You Chen

Faculty, Staff and Student Publications

Objective: This article aims to assess the reproducibility of Manufacturer and User Facility Device Experience (MAUDE) data-driven studies by analyzing the data queries used in their research processes.

Methods: Studies using MAUDE data were sourced from PubMed by searching for "MAUDE" or "Manufacturer and User Facility Device Experience" in titles or abstracts. We manually chose articles with executable queries. The reproducibility of each query was assessed by replicating it in the MAUDE Application Programming Interface. The reproducibility of a query is determined by a reproducibility coefficient that ranges from 0.95 to 1.05. This coefficient is calculated by comparing the number …

Decision-Making In Rats: Effects Of Drug Abuse, Safa Binte Hossain

Open Access Theses & Dissertations

Decision-making is a critical cognitive function, often impaired by substance abuse. Understanding the underlying mechanisms in both human and animal models is essential for developing effective treatments. This thesis aims to study approach-avoid decision-making in rats and focuses on the impacts of oxycodone self-administration and alcohol abuse on approach-avoid decision-making.We hypothesize that oxycodone self-administration will alter decision-making in female rats, revealing sex differences and distinct psychometric functions, alcohol habituation will similarly affect decision-making in male rats. The study involved 23 Long Evans rats (11 males and 12 females) for decision-making behavioral study connecting multiple levels of rewards and costs combinations. …

Electrical Stimulation Of The Dorsal Motor Nucleus Of The Vagus In Male Mice Can Regulate Inflammation Without Affecting The Heart Rate, Aidan Falvey, Santhoshi P Palandira, Sangeeta S Chavan, Michael Brines, Robert Dantzer, Kevin J Tracey, Valentin A Pavlov

Faculty, Staff and Student Publications

Background: The vagus nerve plays an important role in neuroimmune interactions and in the regulation of inflammation. A major source of efferent vagus nerve fibers that contribute to the regulation of inflammation is the brainstem dorsal motor nucleus of the vagus (DMN), as recently shown using optogenetics. In contrast to optogenetics, electrical neuromodulation has broad therapeutic implications. However, the anti-inflammatory effectiveness of electrical stimulation of the DMN (eDMNS) and the possible heart rate (HR) alterations associated with this approach have not been investigated. Here, we examined the effects of eDMNS on HR and cytokine levels in mice administered with lipopolysaccharide …

Impact Of Pretreatment Body Mass Index On The Survival Of Head And Neck Cancer Patients, Zheng Yang, Jobran Mansour, Peng Sun, Peng Wei, Kristina R Dahlstrom, Mark Zafereo, Guojun Li, Neil D Gross

Faculty, Staff and Student Publications

Background: Differences in pretreatment body mass index (BMI) have been associated with survival in squamous cell carcinoma of head and neck (SCCHN). We examined effects of BMI on survival in SCCHN patients after stratifying patients by tumor human papillomavirus (HPV) status and subsite.

Methods: Totally 2204 SCCHN patients in a prospective study were included in this secondary analysis. Multivariable Cox models were used to evaluate associations between pretreatment BMI and overall survival, disease-specific survival, and disease-free survival.

Results: BMI was significantly higher among patients with HPV-positive tumors than HPV-negative tumors. BMI >25 kg/m2 was associated with improved survival, while BMI …

Proteomics In Orthopedic Research: Recent Studies And Their Translational Implications, George Li, Argyrios Stampas, Yoshihiro Komatsu, Xueqin Gao, Johnny Huard, Sheng Pan

Faculty, Staff and Student Publications

Proteomics is a growing field that offers insights into various aspects of disease processes and therapy responses. Within the field of orthopedics, there are a variety of diseases that have a poor prognosis due to a lack of targeted curative therapy or disease modifying therapy. Other diseases have been difficult to manage in part due to lack of clinical biomarkers that offer meaningful insight into disease progression or severity. As an emerging technology, proteomics has been increasingly applied in studying bone biology and an assortment of orthopedics related diseases, such as osteoarthritis, osteosarcoma and bone tumors, osteoporosis, traumatic bone injury, …

Information About Honey Fraud: Implications Of Information Choice For Consumer Behavior, Christopher R. Gustafson

Cornhusker Economics

The study highlights that standard research practice—exposing participants to information—may not capture what will happen outside of the lab when people have a choice about what to read, view, or do with their time. While we found that having participants read about honey fraud significantly shifted their willingness to pay for local and domestic versus imported honey, we did not find those changes when people were able to select a text to read.

The implications of these findings extend beyond the issue of honey fraud to broader contexts of food safety, nutrition, and production techniques. While people have preferences for …

Exploring The Vulnerability Of Small-Scale Fisherfolks In Selected Barangays, Arlon Ponce Cadiz, Blesshelyn Guevarra Sanchez, Eden Vela Evangelista

Journal of Environmental Science and Sustainable Development

Small-scale fisherfolks contributed to the livelihood and economic aspects of the fishery sector in the region. However, they are vulnerable regarding potential risks and threats caused by various conditions. This study explored the vulnerability of fifty-two small-scale fisherfolks selected from six barangays near Laguna's Northern West Bay, Philippines. The case study design guided the data collection from multiple sources, including survey questionnaires, unstructured interviews, and document analysis for triangulation and analysis of fisherfolks' exposure, sensitivity, and adaptive capacity. Results show that there are indicators that contribute to the vulnerability of fisherfolk in terms of exposure, which involves hydrometeorological hazards, water …

Suitability Assessment Of Groundwater Quality For Drinking Purposes, Md. Zahidul Islam, M. G. Mostafa

Journal of Environmental Science and Sustainable Development

The groundwater status of Northwestern Bangladesh is a complex and challenging issue due to its arid and semi-arid climates, increasing population, overexploitation for domestic and irrigation purposes, lack of adequate rainfall, and limited water resources. The study aimed to assess the suitability and health risk of groundwater quality for drinking purposes. A total of one hundred groundwater samples were collected from shallow tubewells in two seasons, the monsoon (MON) and post-monsoon (POM), in northwestern Bangladesh (Ganges River basin) and analyzed using standard methods of analysis (Atomic adsorption spectrometry, UV-visible spectrometry, graphite, ion chromatography). The trends of predominant cations were Ca2+ …

Influence Of Ethanol Concentration On The Extraction Of Cannabinoid And Volatile Compounds For Dry-Hemped Beer, Santiago Cardenas-Pinto, Jacob E. Gazaleh, Drew Budner, Shea Keene, Leena R. Dhoble, Abhisheak Sharma, Brian Pearson, Zhen Jia, Boce Zhang, Katherine A. Thompson-Witrick

Chemistry

Beer is one of the most widely consumed beverages in the world. Since the legalization of low-delta-9-tetrahydrocannabinol (THC) (< 0.03%) Cannabis sativa in the United States, this controversial plant is being looked upon as a potential flavor additive for use in beer. Cannabis sativa shares similar aromatic and flavor characteristics to that of hops (Humulus lupulus). This study was designed to determine the influence ethanol concentration has on the cannabinoid and volatile compounds found in beer. Three experimental beers with varying ethanol (3, 6, and 9% ABV) concentrations were used for this experiment. Using dry-hopping practices and dosing from commercial brewing techniques, hemp was placed in the beer for five days at 25 °C. Cannabinoids and volatile compounds were analyzed following the completion of the dry-hemping process. Statistical differences were observed only for the cannabinoid, tetrahydrocannabinolic acid (THCA), while, no other individual cannabinoid or the summation of the total cannabinoids were statistically different. Volatile compounds were analyzed using gas chromatography–mass spectrometry (GC-MS). Statistical differences were observed between the monoterpenes and the sesquiterpenes volatile compound groups. In summary, ethanol concentration appears to have no impact on the extraction efficiency of cannabinoids but does appear to influence volatile compounds when hemp is added to beer through the dry-hemping process.