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Nitric Oxide Production: A Mechanism For Inhibition Of Chlamydia Trachomatis Replication, Bojun Chen

Electronic Theses and Dissertations

Chlamydia trachomatis (CT) replicates in macrophages, but is inhibited by IFN-$\gamma$ or LPS. IFN-$\gamma$ and/or LPS induced nitrite production in mouse peritoneal macrophages, macrophage cell lines (RAW264.7 and J774A.1) and McCoy cells. Kinetic studies indicated that peak production occurred 48 hours post-treatment. CT infection itself was insufficient to induce nitrite production, but resulted in enhancement of nitrite production in IFN-$\gamma$-treated cells. Treatment with IFN-$\gamma$ or LPS resulted in significant inhibition of CT replication in these cells. Strong correlation between nitrite production and inhibition of CT replication was observed in RAW264.7 and J774A.1 cells (correlation coefficients: $-$0.93 and $-$0.94, p $<$ 0.001). N$\sp{\rm g}$- monomethyl-L-arginine (L-NMMA) specifically inhibited nitrite production and partially reversed inhibition of CT replication in macrophage cell lines. NOS mRNA was measured in RAW264.7 cells by Northern blot and Dot blot hybridization. Strong correlation between NOS mRNA expression and inhibition of CT replication (correlation coefficient: $-$0.97, p $<$ 0.05) was observed. Anti-TNF-$\alpha$ antibody completely neutralized the biological activity of TNF-$\alpha$ secreted by LPS-treated RAW264.7 cells, yet the antibody neither reduced nitrite production nor restored CT replication. Combination of the antibody and L-NMMA significantly enhanced restoration of CT replication. In peritoneal macrophages, inhibition of CT replication induced by IFN-$\gamma$ was partially restored by L-NMMA or anti-TNF-$\alpha$ antibody. In McCoy cells, inhibition of CT replication induced by IFN-$\gamma$ and LPS was not significantly restored by L-NMMA. Great restoration of CT replication by 1 mM L-NMMA was observed in LPS-treated J774A.1 cells (31%), but not in IFN-$\gamma$-treated cells (5%). Our data indicate that (1) NO production is one of the mechanisms for inhibition of CT replication in IFN-$\gamma$-activated peritoneal macrophages and RAW264.7 cells; (2) NO plays a significant role in CT inhibition in LPS-treated macrophage cell lines, but not peritoneal macrophages; (3) TNF-$\alpha$ may be associated with inhibition, but the mechanism(s) may not involve NO production; (4) NO production may not be the mechanism for CT inhibition in McCoy cells treated with IFN-$\gamma$ and LPS.

Agriculture News Release - 1993-11-29, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Agriculture News Release - 1993-11-23, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Agriculture News Release - 1993-11-04, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Agriculture News Release - 1993-11-03, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Wpa News 42 (1993), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (November 1993), number 42

Published by the World Pheasant Association

Nebline, November 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Horticulture

Rural $ense

Human Resources

4-H & Youth

Environmental Focus

Community & Leadership Development

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

Agriculture News Release - 1993-10-27, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Rejex-It™Ag-36, A Potential Tool To Protect Seeds From Bird Depredation, Peter F. Vogt

Wildlife Damage Management Conference

The ever increasing bird populations (e.g., Black birds, geese etc.) are known to cause considerable losses to agriculture. This problem has reached serious proportions for crops that are farmed on large tracts and are seeded by aerial application such as rice and canola. ReJeX-iT™AG-36, a non-toxic, biodegradable bird aversion formulation, derived from food grade ingredients, has been proven in pen tests and field trials to be effective as a seed treatment to prevent birds from eating the treated seeds. The product does not harm the seeds or the effected birds in any way, even if ingested; it just makes the …

Can We Landscape To Accommodate Deer? The Tracy Estate Research Garden, Helen H. Heinrich, Susan Predl

Wildlife Damage Management Conference

The landscape of New Jersey is remarkably rich in vegetation and open space, despite the state's reputation as the nation's most populous state. This landscape is increasingly the product of intense interaction between the white-tailed deer (Odocoileus virginianus) and both native and cultivated vegetation, particularly in suburban communities, where both the whitetail and the vegetation coexist in abundance. Nearly extirpated at the tum of the century due to over-hunting, the state's white-tailed deer population today exceeds 140,000 because habitat is ideal and hunting seasons are carefully regulated. In many instances, where landowners choose not to use hunting as …

Balancing The Needs Of Society: Listening To The Public, Robert H. Schmidt

Wildlife Damage Management Conference

Wildlife damage management, as a professional activity, is involved in a broad range of management issues affecting agricultural productivity and profitability, human health and safety, and endangered and valued species management. Surveys by S. Kellert and others have indicated that the general public is agreeable to resolving wildlife damage issues, even utilizing lethal technologies. However, surveys and case histories have also indicated that the process involved in resolving wildlife damage issues invokes much public concern. By process I mean the specific methodologies, strategies, and resource and social tradeoffs involved in developing and implementing a wildlife damage management program.

Ard News October 1993

Agricultural Research Division: News and Annual Reports

CONTENTS:
COMMENTS FROM THE DEAN
NEW OR REVISED PROJECTS
GRANTS AND CONTRACTS RECEIVED AUGUST AND SEPTEMBER, 1993
PROPOSALS SUBMITTED FOR FEDERAL GRANTS
PROJECT FUNDED BY BOARDS
FY 1994 CSRS BUDGET
ARD MISSION
ARD GOALS AND OBJECTIVES
ARD-MANAGED GRANT PROGRAMS
DR. ALAN DOSTER SELECTED FOR LEADERSHIP DEVELOPMENT COURSE
RESTRICTIONS ON GRADUATE STUDENT FELLOWSHIPS
IMPROVEMENT OF RESEARCH FACILITIES
FEDERAL RESEARCH AND DEVELOPMENT BUDGETS - FY 1992
REPORT ON THE NATIONAL RESEARCH INITIATIVE FOR FY 1992
GREAT PLAINS REGIONAL CENTER FOR GLOBAL ENVIRONMENTAL CHANGE

Nebline, October 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

Weed Awareness Special Edition

Agriculture News Release - 1993-09-30, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Proceedings From The 30th Annual Marschall Italian Cheese Seminar, Various Authors

Cheese Industry Conference

Our interviews with cheese makers, owners, managers, and top management of mozzarella processing companies across the United States resulted in our quest for accurate data from which an automatic Mozzarella processing systems could be designed.

Agriculture News Release - 1993-09-21a, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Agriculture News Release - 1993-09-21b, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Agriculture News Release - 1993-09-09a, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

Ascorbic Acid Is An Abettor In Calcium Urolithiasis: An Experimental Study, P. P. Singh, R. Kiran, A. K. Pendse, Reeta Ghosh, S. S. Surana

Scanning Microscopy

Two sets of animal experiments using guinea pigs were planned to evaluate the effect of ascorbic acid supplementation on the lithogenic process. In the first set of experiments, 10, 40, and 60 mg doses of ascorbic acid/100g body weight/day were given for 105 days. Neither of the ascorbic acid doses given induced crystalluria, calcification or stone formation, thereby confirming our previous findings that ascorbic acid in the doses used by clinicians does not cause urolith formation. In the second set of experiments, ascorbic acid was supplemented in hypercalciuric (induced by calcium carbonate feeding) and hyperoxaluric (induced by sodium oxalate feeding) …

Nebline, September 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

Rural Resource Guide Special Edition

Thelma Flanagan, Thelma Flanagan, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Thelma Flanagan was involved in child nutrition programs in the State of Florida from the 1930s until her death in 2001. She was a strong advocate in the national drive for professionalism in food services. She became the Florida School Food Service Director in 1943, served as school lunch consultant to the USDA, was President of the American School Food Service Association 1949-1950, served as Chairman of the Southern States Work Conference Committee, and was the author of numerous publications regarding school feeding programs.

Proceedings Of The Workshop On Adaptation Of Plants To Soil Stresses, J.W. Maranville, V.C. Baligar, R.R. Duncan, J. M. Yohe

INTSORMIL Impacts and Bulletins

Sustainable production of food and forage with a focus on plant adaptation to stress environments will be a continued priority for developing countries in the future. Since many areas of the world which support substantial human populations are drought prone, such as the subsaharan African zone and others, the primary focus has been on drought. However, one of the greatest restraints to sustainability of agriculture worldwide is the lack of sufficient soil nutrients for crop growth, or other soil constraints such as acidity or salinity which hinder crop production substantially.

Optimizing soil fertility or amending acid and saline soils to …

Nebline, August 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

1993 Lancaster County Fair Edition

Ard News August 1993

Agricultural Research Division: News and Annual Reports

CONTENTS:

FYI994 CSRS BUDGET OUTLOOK
WIDAMAN TRUST DISTINGUISHED GRADUATE ASSISTANT AWARD
ANNUAL REPORT FOR RESEARCH PROJECTS
FORMAT FOR QUALITY ASSURANCE PROJECT PLANS
UNIVERSITY OF NEBRASKA FOUNDATION AWARDS
ARD ADVISORY COUNCIL ELECTION RESULTS
NEW OR REVISED PROJECTS
HARDIN DISTINGUISHED GRADUATE FELLOWSHIP FOR 1993-1994
SUCCESS OF IANR FUNDING
PROPOSALS Submitted
GRANTS AND CONTRACTS RECEIVED JUNE AND JULY, 1993
GROWTH IN TOTAL FEDERAL ACADEMIC SUPPORT
PROJECTS FUNDED BY BOARDS

Wpa News 41 (1993), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (August 1993), number 41

Published by the World Pheasant Association

The Interplay Of Light And The Circadian Clock. Independent Dual Regulation Of Clock-Controlled Gene Ccg-2(Eas), Guiseppina Arpaia, Jennifer J. Loros, Jay C. Dunlap, Giorgio Morelli, Guiseppe Macino

Dartmouth Scholarship

Ambient light is the major agent mediating entrainment of circadian rhythms and is also a major factor influencing development and morphogenesis. We show that in Neurospora crassa the expression of clock-controlled gene 2 (ccg-2), a gene under the control of the circadian clock and allelic to the developmental gene easy wettable (eas), is regulated by light in wild-type strains. Light elicits a direct and important physiological effect on ccg-2(eas) expression as demonstrated using several mutant Neurospora strains. In white collar mutants (wc-1 and wc-2) that are “blind” to blue light, ccg-2(eas) mRNA shows no variation following illumination with saturating light. …

Agriculture News Release - 1993-07-16b, United States. Congress. House. Committee On Agriculture, E. De La Garza

Kika de la Garza Congressional Papers - Agriculture Committee News Releases

No abstract provided.

White-Tailed Deer Habitat Use On Surface-Mined Areas In Eastern Kentucky, Devetta A. Hill

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Devetta A. Hill on July 2, 1993.

Nebline, July 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Home Extension Club News

and other extension news and events

South Dakota Farm And Home Research, South Dakota State University

South Dakota Farm and Home Research: 1949 -1998

Dean's comments: Standing at the door to the future [p] 3
Director's comments: Making the connections [p] 4
Century-old quest for knowledge brought about biostress laboratory [p] 6
Department head comments: Breaking the barriers [p] 10
Department head comments: Accelerating success [p] 12
Department head comments: Expanding our resources [p] 14
Department head comments: Building bridges [p] 15
Department head comments: Sharpening the focus [p] 17
Department head comments: Interaction of stress and animal health [p] 18
The NPB: A powerful new tool in biostress research [p] 20
The complexities of biostress [p] 22
The long battle with biostress [p] …