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Responses Of Medical Cannabis To Daily Light Integrals Higher Than Summer Sunlight: Yield, Morphology, And Quality, James D. Nielson

All Graduate Theses and Dissertations, Fall 2023 to Present

Cannabis sativa L. is a crop with growing medical and commercial significance. However, cannabis is not as well studied or understood as many of the most common crops. A novel area of research surrounds how much light the plant can tolerate or use to keep increasing flower yield. In agriculture, light intensity is often measured by how many light particles interact with a given area in a second, referred to as photosynthetic photon flux density (PPFD). For example, at Utah State University in Logan, Utah, during the peak of summer, the sun's light intensity can reach about 2000 μmol·m^{-2 …}

Chronic Β3-Ar Stimulation Activates Distinct Thermogenic Mechanisms In Brown And White Adipose Tissue And Improves Systemic Metabolism In Aged Mice, Duraipandy Natarajan, Bhuvana Plakkot, Kritika Tiwari, Shoba Ekambaram, Weidong Wang, Michael Rudolph, Mahmoud A Mohammad, Shaji K Chacko, Madhan Subramanian, Stefano Tarantini, Andriy Yabluchanskiy, Zoltan Ungvari, Anna Csiszar, Priya Balasubramanian

Faculty, Staff and Students Publications

Adipose thermogenesis has been actively investigated as a therapeutic target for improving metabolic dysfunction in obesity. However, its applicability to middle-aged and older populations, which bear the highest obesity prevalence in the United States (approximately 40%), remains uncertain due to age-related decline in thermogenic responses. In this study, we investigated the effects of chronic thermogenic stimulation using the β3-adrenergic (AR) agonist CL316,243 (CL) on systemic metabolism and adipose function in aged (18-month-old) C57BL/6JN mice. Sustained β3-AR treatment resulted in reduced fat mass, increased energy expenditure, increased fatty acid oxidation and mitochondrial activity in adipose depots, improved glucose homeostasis, and a …

Random C-Peptide And Islet Antibodies At Onset Predict Β Cell Function Trajectory And Insulin Dependence In Pediatric Diabetes, Mustafa Tosur, Saima Deen, Xiaofan Huang, Serife Uysal, Marcela Astudillo, Richard A Oram, Maria J Redondo, Farook Jahoor, Ashok Balasubramanyam

Children’s Nutrition Research Center Staff Publications

Objective: Identification of prognostic biomarkers in pediatric diabetes is important for precision medicine. We assessed whether C-peptide and islet autoantibodies are useful to predict the natural history of children with new-onset diabetes.

Methods: We prospectively studied 72 children with new-onset diabetes (median follow-up: 8 months) by applying the Aβ classification system ("A+": islet autoantibody positive, "β+": random serum C-peptide ≥1.3 ng/mL at diagnosis). Beta-cell function was assessed longitudinally with 2 hours postprandial/stimulated urinary C-peptide-to-creatinine ratio (UCPCR) 3-12 weeks (V1) and 6 to 12 months after diagnosis (V2). We obtained a type 1 diabetes genetic risk score for each participant, and …

Association Of Late Eating With Colorectal Adenomas: A Cross-Sectional Study, Darbaz Adnan, Edena R Khoshaba, Mostafa Abel-Reheem, Jonathan Q Trinh, Yin Cao, Faraz Bishehsari

Faculty, Staff and Student Publications

Purpose: Colorectal cancer (CRC) is linked to lifestyle exposures. However, changes in the CRC rates among younger populations remain poorly understood and suggest the existence of yet unidentified factor(s) that may contribute to colon carcinogenesis. Here, we investigated the potential role of time of eating in the risk of pre-cancerous colonic neoplasms (tubular adenoma: TA).

Methods: We enrolled 663 participants undergoing screening colonoscopies. Data on food timing, dietary intake, sleep/wake patterns, and chronotype were collected through structured questionnaires. Late eating was defined as the consumption of food or snack within a 3-hour window of sleep onset for at least four …

Hunting For Heroes: Brain Neurons Mediating Glp-1r Agonists In Obesity Treatment, Yuhan Cao, Qingchun Tong

Faculty, Staff and Student Publications

Glucagon-like peptide 1 (GLP-1) receptor agonists (GLP-1RAs) have proven to be highly effective in reducing obesity across species and ages, gaining unmet popularity in clinical treatments against obesity. Although extensive research efforts have been made to explore how the brain regulates body weight homeostasis including the effect brought up by GLP-1 and its synthetic analogs GLP-1RAs, the identity of neurons and neural pathways that are responsible for the observed anti-obesity effect of GLP-1RAs remain largely elusive. Excitingly, three recent high-profile studies presented compelling evidence that each argues for the importance of GLP-1Rs in the dorsomedial hypothalamus, hindbrain, or lateral septum, …

Predictive Model For Listeria Monocytogenes In Rte Meats Using Exclusive Food Matrix Data, N.A. Nanje Gowda, Manjari Singh, Gijs Lommerse, Saurabh Kumar, Eelco Heintz, Jeyamkondan Subbiah

Food Science Faculty Publications and Presentations

Post-processing contamination of Listeria monocytogenes has remained a major concern for the safety of ready-to-eat (RTE) meat products that are not reheated before consumption. Mathematical models are rapid and cost-effective tools to predict pathogen behavior, product shelf life, and safety. The objective of this study was to develop and validate a comprehensive model to predict the Listeria growth rate in RTE meat products as a function of temperature, pH, water activity, nitrite, acetic, lactic, and propionic acids. The Listeria growth data in RTE food matrices, including RTE beef, pork, and poultry products (731 data sets), were collected from the literature …

The Impact Of In Ovo Administration Of Probiotic Lactobacilli On The Gut Health And Immune Competence Of Broiler Chickens, Shreeya Sharma

All Theses

Due to the incomplete development of their immune system, newly hatched chicks are vulnerable to a wide range of pathogenic microorganisms, including enteric bacterial pathogens during the early stage of life. To boost their immune system during this critical period, numerous studies suggest early feeding, either in ovo or immediately post-hatch, of nutrients and immunomodulators such as prebiotics, probiotics, phytobiotics, Toll-like receptors (TLRs) ligands, vitamins and minerals. Probiotics, defined as live, non-pathogenic microorganisms that confer beneficial effects on the host, have been extensively studied for their effects on poultry health. Supplementation of probiotics to chickens has demonstrated a broad spectrum …

Evaluation Of Pierce’S Disease-Resistant Grapevine Cultivars In Upstate South Carolina, Annie Bruno

All Theses

Interest in wine grape production is increasing in South Carolina, but Pierce’s Disease (PD) caused by the xylem-limited bacteria Xylella fastidiosa (Xf) is a major limiting factor. Xf is spread by sap-sucking insect vectors in the Cicadellidae family such as sharpshooters. In the United States, Xf has become endemic to the southeast as global temperatures rise, causing winter temperatures to increase, which allows the bacteria to survive year-round. However, PD-resistant grapevine hybrid cultivars have become available through breeding V. vinifera genes with native American Vitis species that are resistant to Xf. In this study, the PD-resistant grapevine …

Sustainable Agriculture Practices And Economic Outcomes: Selected Ipm In Tomato And Cover Crop Inter-Seeding In Organic Corn In South Carolina, Rohini Sonti

All Theses

This two-chapter thesis focuses on the economic evaluation of integrating a combination of sustainable agricultural practices aimed at improving environmental resilience and farm profitability across both conventional and organic farming systems in South Carolina. The research study lays emphasis on two key areas: the use of Integrated Pest Management practices, specifically grafting and Anaerobic Soil Disinfestation (ASD) in tomato production and the inter- seeding of cover crops under different tillage systems in organic corn production.

The first chapter assesses the economic feasibility of two IPM practices: grafting and anaerobic soil disinfestation (ASD) in tomato production. Following the phase-out of methyl …

The Effect Of Packaging On The Acceptance Of Natural Peanut Butter, Perian M. Reese

All Theses

The aim was to determine the effect of packaging on the acceptance of natural peanut butter, especially concerning the acceptance of the undesirable oil layer. The jars of peanut butter differed by color, label size, and inclusion of qualifying statements. 119 participants across two groups completed this study. Both groups completed an eye-tracking study in a mock grocery setting. The first group rated all five jars in appearance, expected quality, and overall rating. They were given one sample cup of peanut butter and gave hedonic liking values for color, oiliness, peanut flavor, smoothness, sweetness, and overall liking. The second group …

On Humans, Hogs, And Habitat: How Landscape Features And Vegetation Influence Wild Hog (Sus Scrofa) Detection On Private Lands And Using Existing Wild Hog Outreach Websites To Create An Effective Website For South Carolina, Andrew Jamison

All Theses

Invasive species are a source of human conflict with wildlife on a global scale and exist to the detriment of native wildlife species. Invasive species often possess the ability to respond rapidly to change and they often exist alongside humans. Wild hogs (Sus scrofa) are perhaps one of the most infamous examples of an invasive species. The spread of wild hogs necessitates effective communication with the public being impacted by them, as well as a continuation of research to learn more about wild hog behavior to promote effective management. Chapter 1 focuses on how we created a website …

Developing Holistic And Reduced Fertilization Guidelines For Southeastern Peach (Prunus Persica (L.) Batsch) Orchards, Richardson Bien-Aime

All Theses

Peach production in the southeastern United States requires optimizing fertilization practices to balance economic sustainability and environmental health. Current guidelines may overlook rootstock-specific nutrient requirements and fail to align with orchard-specific conditions, often resulting in overfertilization, increased costs, and environmental risks. This study, conducted at Clemson University’s Musser Fruit Research Center during 2023 and 2024, aimed to refine fertilization practices through two complementary experiments. The first experiment investigated how three rootstocks, ’MP-29’, Lovell, and Guardian, influence nutrient dynamics of ‘Carored’ peach trees. Nutrient concentrations were measured in various tree organs, including leaves, pruned wood, thinned fruitlets, and harvested fruits, along …

Field Prevalence Of Ring Nematodes In South Carolina And Its Potential To Predispose Peanut Pods To Aflatoxin Contamination, Sarala Giri

All Theses

Peanut ring nematode, Mesocriconema ornatum is a nematode pest of peanut which can cause necrotic lesions on pods, pegs, and roots. It has been reported to facilitate secondary infections in peanut and other crops resulting in more significant losses in yield and quality. Aspergillus flavus is a ubiquitous facultative saprophyte that produces aflatoxin, the most problematic mycotoxin for global health and economy. Experiments were conducted at the Edisto Research and Education Center of Clemson University to examine the relationship of ring nematode populations at pegging and harvest with aflatoxin concentration in peanut kernels due to infection by A. flavus …

Targeting The Glutamine-Arginine-Proline Metabolism Axis In Cancer, Di Wang, Jiang-Jie Duan, Yu-Feng Guo, Jun-Jie Chen, Tian-Qing Chen, Jun Wang, Shi-Cang Yu

Faculty, Staff and Student Publications

Metabolic abnormalities are an important feature of tumours. The glutamine-arginine-proline axis is an important node of cancer metabolism and plays a major role in amino acid metabolism. This axis also acts as a scaffold for the synthesis of other nonessential amino acids and essential metabolites. In this paper, we briefly review (1) the glutamine addiction exhibited by tumour cells with accelerated glutamine transport and metabolism; (2) the methods regulating extracellular glutamine entry, intracellular glutamine synthesis and the fate of intracellular glutamine; (3) the glutamine, proline and arginine metabolic pathways and their interaction; and (4) the research progress in tumour therapy …

Integrated Transcriptomics And Proteomics Study Of Starvation Selected Drosophila Melanogaster, Aavash Adhikari

UNLV Theses, Dissertations, Professional Papers, and Capstones

Drosophila melanogaster, selected for starvation resistance exhibits phenotypic traits such as increased body size, slower development, reduced fecundity and obesity like characteristics including higher lipid reserves and cardiac dysfunction. While prior studies have explored the metabolic and biochemical changes in these flies, the underlying genetic and molecular mechanisms remain unclear. This study aims to investigate the gene expression and protein abundance in starvation-selected Drosophila through RNAseq, proteomics, and integrating both datasets using the MixOmics package in R. I found differential gene expression in the fat body at the third instar larval stage, particularly in pathways related to lipid metabolism, calcium …

Understanding Failure Mechanisms For Two Colorectal Anastomosis Techniques, Youssef Sherif Sadek Fahmy

UNLV Theses, Dissertations, Professional Papers, and Capstones

Colorectal Surgery continues to be one of the most common gastrointestinal surgeries performed in the US. A common complication known as Anastomotic Leakage (AL) that threatens up to 30% of patients with this surgery. Many cases of AL can decrease the quality of life for patients or extensively extend hospital stays, and in many cases, lead to death. Researchers have studied this complication in clinical trials, yet few studied the mechanics of stapled anastomosis in controlled environments. The purpose of this research was to understand the mechanical interaction between the staples and the tissue, and to eventually apply it in …

Validation Studies Of The Flash-Tv System To Passively Measure Children’S Tv Viewing, Anil Kumar Vadathya, Tatyana Garza, Uzair Alam, Alex Ho, Salma M A Musaad, Alicia Beltran, Jennette P Moreno, Tom Baranowski, Nimah Haidar, Sheryl O Hughes, Jason A Mendoza, Ashok Veeraraghavan, Joseph Young, Akane Sano, Teresia M O'Connor

Children’s Nutrition Research Center Staff Publications

TV viewing is associated with health risks, but existing measures of TV viewing are imprecise due to relying on self-report. We developed the Family Level Assessment of Screen use in the Home (FLASH)-TV, a machine learning pipeline with state-of-the-art computer vision methods to measure children's TV viewing. In three studies, lab pilot (n = 10), lab validation (n = 30), and home validation (n = 20), we tested the validity of FLASH-TV 3.0 in task-based protocols which included video observations of children for 60 min. To establish a gold-standard to compare FLASH-TV output, the videos were labeled by trained staff …

Household Food Expenditure Typology And Its Association With Child Stunting And Anemia In Indonesia, Ratu Ayu Dewi Sartika, Yusnita Yusnita, Winda M. Ningsih, Ririn Arminsih Wulandari, Siti A. Pujonarti, Fadila Wirawan

Kesmas

Stunting and anemia remain the coexisting nutritional problems among Indonesian children. However, evidence of household-level food expenditure and its association with child stunting and anemia is limited. This study aimed to observe the association between household food expenditure typology and child stunting and anemia in Indonesia. Data was obtained from the 2007 and 2014 Indonesia Family Life Survey (IFLS). The household food expenditure, child nutritional status, and anemia were observed in 2007 and 2014. Latent class analysis was taken to identify the food expenditure pattern. Binary logistic regression was applied to analyze the association between household food expenditure patterns and …

Dietary Diversity As A Dominant Factor Of Energy Intake Among Breastfeeding Mothers In Depok City, Indonesia, Cornelia Lugita Santoso, Ratu Ayu Dewi Sartika, Sandra Fikawati, Fadila Wirawan, Primasti Nuryandari Putri, Nurul Husna Mohd Shukri

Kesmas

Maternal nutritional needs increase during lactation. However, the energy intake of breastfeeding mothers usually still falls short of the recommended adequacy levels. This study aimed to identify the dominant factor of energy intake in breastfeeding mothers in Sawangan District, Depok City, Indonesia. This quantitative study used a cross-sectional design from July 2022 to February 2023. Energy intake, dietary diversity, and eating frequency were collected using 24-hour food recall. Other variables were collected through interviews with structured questionnaires. Data were analyzed using the Chi-square test and binary logistic regression. A total of 217 breastfeeding mothers participated in this study. This study …

Influence Of Acute High-Intensity Resistance Exercise On Cognitive Performance And Prefrontal Cortex Oxygenation In Recreational Weightlifters, Samuel M. Camacho, Terence Moriarty, Mick G. Mack, Jacob P. Reed, Hyeyoung Cho

Topics in Exercise Science and Kinesiology

Acute exercise has been shown to be effective in improving post-exercise cognitive performance, including executive function. In addition, changes in prefrontal cortex (PFC) activation have been linked to any such improvements. Since resistance and higher intensity exercise is much less studied, the aim of this study was to examine the effect of high-intensity resistance exercise on cognitive performance and to determine if PFC activation was related to this cognitive performance in recreationally trained weightlifters. Ten (M = 5, F = 5) college aged (22 ± 2 years) recreationally trained weightlifters completed the Stroop task prior to and 15- and 30-min …

Variation In Diet And Leafy Browse Offerings Amongcolobus Monkeys (Colobus Spp.) And Langurs(Trachypithecus Spp.) In Aza Facilities, Maura R. Plocek, Noah T. Dunham

Student Scholarship

Providing nutritionally balanced diets is a key aspect of successful husbandry and can help support sustainable ex situ populations. This study focuses on the diets of zoo‐housed colobus monkeys (Colobus angolensis and C. guereza) and langurs (Trachypithecus cristatus and T. francoisi) across AZA institutions. We utilized survey responses from 34 institutions to (1)document the extent of dietary variation with regard to food categories and macronutrients offered, (2) assess interspecific variation in diets offered, and (3) identify important aspects of browse provisioning among institutions. A total of 95 different food items and 229 common‐name browse types were …

Association Mapping For Water Use Efficiency In Soybean Identifies Previously Reported And Novel Loci And Permits Genomic Prediction, Siva K. Chamarthi, Larry C. Purcell, Felix B. Fritschi, Jeffrey D. Ray, James R. Smith, Avjinder S. Kaler, C. Andy King, Jason D. Gillman

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Soybean is a major legume crop cultivated globally due to the high quality and quantity of its seed protein and oil. However, drought stress is the most significant factor that decreases soybean yield, and more than 90% of US soybean acreage is dependent on rainfall. Water use efficiency (WUE) is positively correlated with the carbon isotopic ratio ¹³C/¹²C (C13 ratio) and selecting soybean varieties for high C13 ratio may enhance WUE and help improve tolerance to drought. Our study objective was to identify genetic loci associated with C13 ratio using a diverse set of 205 soybean maturity …

Randomized Pilot Study Examining An Online Diet Intervention To Increase Intake Of Fruits And Vegetables Among Healthy Adults, Diana Kolb, Sarah Deemer, Eva Claire Synkowski, Stephanie Silveira

International Journal of Exercise Science

International Journal of Exercise Science 17(2): 1618-1628, 2024. Intake of fruits and vegetables (F&V) is related to health outcomes. The purpose of the study was to test an online diet program promoting F&V intake among healthy adults. Twenty-three participants were randomly assigned to complete an 8-week intervention condition (#800gChallenge®) or wait-list control condition. In weeks 1-4, intervention participants were asked to interact with an application reporting (a) whether they met their daily 800g F&V goal, (b) if their 800g of F&V was from a variety of sources, and (c) reading of daily educational material. Weeks 1-4 included weekly Zoom check-in …

Student Comprehension Of Biochemistry In A Flipped Classroom Format, Ed Harris, Evan A. Schroder, Teryn J. Berks

Department of Biochemistry: Faculty Publications

Concept-heavy courses such as Biochemistry in life and physical science curricula are challenging for many college-aged students. It is easy for students to disengage in a lecture and not learn the subject matter while in class. To improve student learning and participation, we employed a flipped format for the first half of the course and compared learning outcomes and attitudes with the traditional lecture in the second half of the course. The experimental course was an upper-level biochemistry course taken primarily by juniors (49%), seniors (40%), and some graduate students (10%) at the college level. Our results indicate that lower-performing …

Cultivation Of Food And Fodder Crop Millets And Quinoa In The Aquaponics System For Sustainable Food Security In The United Arab Emirates, Salama Yousef Alkhoori

Thesis/ Dissertation Defenses

Aquaponics is a sustainable food production technology that integrates aquaculture with hydroponics. This study aimed to evaluate the possible impact of an aquaponics system on the cultivation of pearl millet, finger millet, and quinoa, utilizing water from tilapia aquaculture. The duration of the experiment is six months utilizing the manually constructed backyard aquaponics system. Tilapia fish serves as the principal biological material for nutrient generation in the experiment. This study investigated and recorded water quality indicators, including pH, temperature, dissolved oxygen, electrical conductivity, total dissolved solids, ammonia, nitrite, and nitrate levels over the experimental period. The parameters of fish growth, …

The Association Of Zero Walking Cadence Minutes With Sleep Quality In Adults 18 – 65, Trent A. Hargens, Matthew C. Scott, Meghan Peterson, Kimberly C. Bennett, Timothy Thome, Elizabeth S. Edwards

Faculty Scholarship

Purpose Physical activity (PA) guidelines suggest a link between PA, sedentary time, and sleep quality (SQ). Step cadence is an emerging proxy for PA intensity, with zero cadence (ZC) indicating sedentary time. No research has explored the relationship between ZC and SQ. This study examines the relationship between ZC and SC, compared to traditional PA and sedentary metrics, all measured via accelerometry. Methods Sleep and PA was assessed in 138 adults (53 male, 85 female, age = 23.5 ± 8.0 year.) via accelerometry. Sedentary, light, moderate, vigorous intensity PA, and ZC minutes per day were measured. SQ variables included sleep …

Assessment And Prioritization Of Contaminants Of Concern Along Florida's Coral Reef, Ellen Regina E. Skelton

All HCAS Student Capstones, Theses, and Dissertations

Florida’s Coral Reef (FCR) is the third largest barrier reef in the world and faces significant stress from localized land, water, and air-based sources of pollution. Numerous chemical contaminants have been found in waters surrounding reefs and within corals, including pesticides, hydrocarbons, personal care products, pharmaceuticals, and trace metals. However, for most current and emergent environmental contaminants, little to no consistent information exists regarding environmental concentrations or toxicological effects on corals. Assessment of the levels of contaminants in coral reef environments is needed to conduct risk assessments to guide management, restoration, and recovery strategies, and the coral reef resource management …

Study On The Seasonal Endobacterial Profile Of Avicennia Officinalis, Focusing On Core Bacterial Consortia Within Mangrove Pneumatophores, Devi R R, Vinod Prabhakar

Karbala International Journal of Modern Science

Microhabitats are smaller pockets of ecosystems with unique conditions and they serve as major contributors of species richness. Mangrove plants offer a diverse array of such microhabitats (rhizosphere, phyllosphere, endosphereetc), and the inhabitant bacteria benefit the host by providing better salinity tolerance, faster and better nutrient mobilization, protection from phytopathogens and assistance in seed germination. Identification of such beneficial bacteria,their growth requirementsandmetabolism, willhave direct application in the field of agriculture and ecosystem restoration. Hence in this study, effort has been made to elucidate the seasonal numerical and compositional profile of pneumatophore bacterial consortia of Avicennia officinalis, from the mangrove …

Γ -Aapeptides Targeting Amyloid-Β Pathway And Neural Repair For Alzheimer’S Disease Treatment, Ning Shen

USF Tampa Graduate Theses and Dissertations

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid-beta (Aβ) plaques and progressive neuronal degeneration. Despite extensive research, no treatments have been able to effectively halt or reverse the progression of the disease. This study aimed to evaluate the therapeutic potential of two novel γ-AApeptides, HW-C9 and 125-6b, in AD treatment, assessing both their individual and combined effects. HW-C9 was designed to inhibit Aβ aggregation, while 125-6b was developed to promote neuronal growth and repair. Initially, the therapeutic effects of HW-C9 and 125-6b were assessed both in vitro and in vivo. The results showed that HW-C9 …

Metagenome-Assembled Genomes Of Pig Fecal Samples In Nine European Countries: Insights Into Antibiotic Resistance Genes And Viruses, Boxuan Yang, Jianbo Yang, Routing Chen, Jianmin Chai, Xiaoyuan Wei, Jiangchao Zhao, Junxiang Zhao, Feilong Deng, Ying Li

Animal Science Faculty Publications and Presentations

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