Digital Commons Network^™

The Effects Of Exosomes In Lead And Lps Induced Inflammation In Microglia, Yitzchak Lax

Theses and Dissertations

Exosomes are membrane bound subcellular vesicles formed through membrane budding of a cell and play important roles in cell-cell communication. Studies have demonstrated inflammatory changes in various cell types depending on the etiology of the exosomes such as exosomes from cancer cells or exosomes derived from mesenchymal stem cells. The purpose of this study was to determine the inflammatory consequences of exosomes in microglial cells following exposure to lead, lipopolysaccharides (LPS) and lead+LPS. The cultured mouse microglial cells were treated at 0.01 μM, 0.1 μM and 1 μM of lead,- three environmentally relevant levels and LPS was used at 10 …

Analysis And Authentication Of An Optical Method For Early Detection Of Harmful Algal Blooms, Cody Schumacher

Theses, Dissertations and Capstones

The proliferation and frequency of harmful algal blooms (HABs) attributed to eutrophication, storm events and a changing climate have been an increasing concern in both lotic and lentic freshwater ecosystems. Methods of detecting HABs have been explored through fluorescent measurement and sample analysis, but are often expensive and time-consuming. A novel smart device application is in development to detect HABs based on images captured and analyzed by machine learning algorithms trained to distinguish potential cyanobacterial blooms. The HABs App model accurately detected cyanobacteria in strong relationship with biovolume concentrations (R² = 0.996) within the Greenup Pool of the Ohio …

Soil Amendments Alter Tree Growth And Wood Decay After Forest Thinning, Deborah S. Page-Dumroese, Martin Jurgensen, Chris Miller, Joanne M. Tirocke, Derek N. Pierson, Cole Mayn, Mark J. Kimsey, Haley C. Anderson

Michigan Tech Publications

Forest soil amendments are increasingly used in western US forests to dispose of unmerchantable woody residues, reduce wildfire risk, and improve soil properties. Our objective was to determine the effect of fertilizer and organic amendments on tree growth and organic matter decomposition after thinning. Treatments were a control, three single soil amendments (wood chips, fertilizer, and biochar), and one combined soil amendment (biochar + fertilizer), each applied after thinning a ponderosa pine (Pinus ponderosa Dougl. ex. Laws) stand. After 10 years, amendment treatments had no effect on tree diameter increment (p = 0.600), but the biochar + fertilizer and wood …

Using Neuroscience To Understand Food, Tyler Thronton, Jonathan Kershaw

Library/Life Sciences Undergraduate Poster Competition 2025

Food liking is determined by evaluation and reward centers in the brain. Few studies have observed the magnifying effects that messages have on taste liking as participants try food in a neuroimaging setting.

A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren

Masters Theses & Specialist Projects

Spider beetles are small, often flightless insects that occur in hyperarid environments around the world. They exhibit interesting morphology and behavior that allows them to conserve water they obtain from plant material, dung, and other detritus. One hyperarid region in which they are found is the Pacific coast of Perú and northern Chile. The Sechura Desert, Peruvian Coastal Desert, and Atacama Desert make up the arid coast where spider beetles occur from north to south. Through multiple years of field work and museum collections, populations of spider beetles were found along the coast in isolated pockets of vegetation, separated by …

Effect Of Ph And Iron Chelate On The Growth Of Basil And Soybean In Soilless Media, Christian Baker, Bruce Bugbee

Nutrients

Iron (Fe) deficiencies are common in alkaline root zones and can cause chlorosis and reduce growth. The most common approach to prevent deficiency is using synthetic chelates that keep iron in solution at high pH. Here we report the effects of pH and chelate type on the development of chlorosis and biomass in soybeans and basil. We grew the plants at a pH ranging from 6.0-7.8. Fe was chelated with either a less expensive EDTA chelate or a more expensive EDDHA chelate. There was no significant difference between chelates at pH 6.0 in either soybeans or basil. In soybeans, there …

Characterization Of Legionella Pneumophila Effector Protein Ceg10, Emmanuella Ofoka

Masters Theses & Specialist Projects

Legionella pneumophila, the causative agent of Legionnaires' disease, utilizes a Type IV secretion system (T4SS) to transport effector proteins into host cells. This bacterium is said to have over 300 effector proteins. Among these, the Ceg10 effector protein had remained uncharacterized, despite evidence suggesting the importance of bacterial effector proteins in the host-pathogen interaction. This study aimed to perform a structural and functional characterization of Ceg10 through transcriptomic, bioinformatics, and molecular analyses. Transcriptomic profiling of HEK 293T cells expressing Ceg10 revealed significant differential gene expression, with approximately 20 upregulated genes, including leucine-rich repeat, as well as other downregulated genes. …

Coffee Farmgate Price And Post-Harvest Processing In Honduras., Isaac Desouza

Theses and Dissertations--Agricultural Economics

Volatile commodity markets and changing climate expose coffee farmers to price and production risk. Proponents recommend specialty coffee as a strategy to combat price risk, because its unique attributes differentiate it from commodity coffee. Extrinsic and intrinsic attributes define specialty coffee and affect prices at many levels of the supply chain. Despite much work on extrinsic attributes, little is known about how intrinsic attributes relate to farmgate price. Post-harvest processing is itself an intrinsic trait and driver of coffee quality. In 2024 we surveyed 261 coffee farmers from La Paz, Honduras, to uncover relationships between capital, post-harvest processing, and farmgate …

"Dental Health For Mental Wealth": A Comprehensive Analysis Of The Underexplored Impact Of Oral Health And Behaviors On Neurobiology And Psychological Well-Being, Brooke C. Beck

Mahurin Honors College Capstone Experience/Thesis Projects

This thesis explores the link between oral health and psychological state, specifically, the effect of oral health on psychological state and how appropriate oral health impacts this relationship. A review of the literature was conducted and studies interpreted within the scope of oral health’s impact on psychological health and detailing some of the pathways proposed for such a link. The literature search was limited to the English language from well-known databases mainly from 2015 to 2025. A few outliers are present to include foundational information.

The effects of oral disease and oral self-care behaviors were dissected through a biochemical perspective, …

Layer Industry Update: Nutrition Associated With Changes In Production Systems, Kenneth E. Anderson, Ishab Poudel

Proceedings of the Arkansas Nutrition Conference

This is not a hardcore research presentation but rather an overview of three North Carolina Layer Performance and Management Tests. I used the 39th, 40th, and 41st Layer Test Reports that cover the years 2013 through 2025. The proceedings cover white and brown egg strains as groups and the cage and cage-free production environments. Over all three of these tests the production environments were the same and met UEP Cage standards and the Humane Farm Animal Care cage-free standards. There are papers outlining the changes in more detail with all the statistics. This is an overview of visual trends in …

Field Experience And Production Challenges In Australia And New Zealand, Nick Rodgers

Proceedings of the Arkansas Nutrition Conference

Variability management represents the primary technical challenge facing the Australian and New Zealand (ANZ) broiler industry's vertically integrated operations. This analysis examines systematic approaches to controlling seasonal performance fluctuations, raw material quality variations, emerging disease pressures, and evolving welfare demands. Key variability sources include climatic extremes, season to season raw material quality and nutrient value variability and intermittent [mostly enteric] disease challenges, particularly in no antibiotics ever (NAE) production systems. Solutions require a scientific, data-driven approach with insights from field experience for causation identification and a collaborative approach within business functional units, and across industry collaborators such as suppliers and …

Propelling Whole-Cell Biosensor Creation By Leveraging Gene Fusion To Assemble Synthetic Functional Transcription Factors For The Detection Of Biomarkers, Samuel J. Verzino

Theses and Dissertations

Biosensors are analytical devices that leverage biological modules for the detection of molecules of interest. As customizable tools designed to aid in a wide array of diagnostics, these instruments are rapidly advancing the field of synthetic biology. While varied in their construction and purpose, biosensors are ultimately concerned with converting a physicochemical stimulus into a detectable signal. Among the many existing variants, whole-cell biosensors (WCBs) stand out due to their versatility and engineering potential. WCBs are a type of biosensor in which a physiologically active cell sustains an array of biomolecular elements - receptors, transporters, regulators, etc. - capable of …

Climate And Gypsum Parent Material Shape Biocrust Communities And Moss Ecology In The Chihuahuan And Mojave Deserts, Katelyn Gobbie, Nicole Pietrasiak, Brian M. Jusko, Rebecca E. Drenovsky

2025 Faculty Bibliography

Biological soil crust communities (biocrusts) establishing on gypsum soils have been well-documented for their prolific appearance and rich diversity of lichens and bryophytes. However, studies characterizing gypsum biocrusts have occurred primarily outside of the U.S., most of which lack comparisons to other soil types. We conducted intensive field surveys to evaluate the ground cover and frequency of biocrust functional groups and moss species on gypsum and non-gypsum soils in the U.S. regions with the most extensive gypsum outcrops, the northern Chihuahuan and eastern Mojave Deserts. Study sites were stratified by geomorphology and paired, so that every gypsum site was matched …

Assessing The Foraging Habits Of Two Species Of Long-Horned Bees (Melissodes, Apidae) Using Dna Barcodes Its2 And Trnl, Rebecca Jane Foy

Theses, Dissertations and Capstones

Sunflowers (Helianthus, Asteraceae) are an important crop used for oil, dye, fodder, and edible seeds. Long-horned bees (Melissodes, Apidae) are solitary species native to the Americas that specialize in sunflower pollination; they are among the most frequent visitors to sunflowers, and have been shown to enhance yield, offering considerable economic benefits to sunflower production. This study used metabarcoding to investigate the foraging habits of two understudied Melissodes species, M. trinodis and M. agilis. Using the ITS2 and trnL barcodes, our study aimed to (1) investigate the plants visited by M. agilis and M. trinodis and (2) assess the distances Melissodes …

Behavioral And Physiological Responses Of Heifers With No Prior Exposure And Those Adapted To Grazing Endophyte-Infected Tall Fescue, Tyler A. Purvis

Theses and Dissertations--Animal and Food Sciences

Data from year one of a three-year experiment was used to determine behavioral and physiological responses of heifers adapted to grazing endophyte-infected fescue (E+TF) compared to heifers naïve to grazing E+TF. In May, yearling beef heifers from KY (n=15) and MT (n=30) were stratified by body weight, hair coat score, and disposition into nine pastures and assigned to one of three treatments: KY grazing E+ (KY-E+), MT grazing E+ (MT-E+), or MT grazing endophyte-free tall fescue (E-TF) (MT-E-). Prior to grazing, two heifers per pasture were selected to be outfitted with a RumiWatch® halter (Itin+Hoch GmbH, Liestal, Switzerland) for monitoring …

Assessing Land Suitability For Agroforestry, Huseyin B. Tecimen

Extension Publications

No abstract provided.

Nutrition Security – Why It Matters?, Priscilla Ayine, Veronica Oates

Extension Publications

No abstract provided.

Antimicrobial-Resistant Enterococcus In Urban Communities: Insights Into Human Infection Risks, Sumit Kumar Paudel

Wayne State University Dissertations

The complexity of community risk factors and transmission routes has been a major challenge to the containment of community-acquired antibiotic resistance. Possible factors range from environmental contamination, antibiotic misuse, and human exposure via animals, food, the environment, and human-to-human transmission. What has been missing from previous research is the integration of findings in animal and environmental sectors with bacteria implicated in community infections. To gain a better understanding of antibiotic resistance acquired in the community, a one health approach is clearly needed. Specifically, we aim to investigate the prevalence of antibiotic-resistant Enterococcus in wildlife, their gene profiles in comparison with …

Potential Of Anogenital Distance As A Genetic Selection Trait In Canadian Holstein, G.R. Dodd, F.S. Schenkel, F. Miglior, T.C. Bruinjé, M. Gobikrushanth, J.E. Carrelli, M. Oba, D.J. Ambrose, C.F. Baes

Dairy Science and Food Science Faculty Publications: 2022 to Present

Maintaining optimal fertility in dairy cattle herds is a global challenge that is typically addressed through the genetic selection of fertility indicator traits. However, many of the traits currently implemented in breeding programs are heavily influenced by environmental factors, resulting in a slow rate of genetic improvement. Anogenital distance (AGD) has recently emerged as a promising fertility indicator trait due to its association with favorable reproductive outcomes and its higher heritability estimates compared with currently evaluated traits. This study aimed to enhance the understanding of AGD's genetic potential by estimating its genetic parameters in Canadian Holsteins, assessing the reliability …

Science, Physiology, And Nutrition For The Nonscientist, Judi S. Morrill

Open Educational Resources

A wonderful blend of physiology, nutrition, biochemistry, genetics, biology, evolution, chemistry--what we all need to know as informed citizens. A basic knowledge of the life sciences and how our bodies work--to promote our own good health, especially as we're bombarded with misleading advertisements, soundbites, and the like. DNA fingerprinting, calorie requirements, dietary advice, genetic engineering (including gene editing with CRISPR cas9)--all in an easy-to understand book.

Carbohydrate Counting: Traditional Filipino Food Lists For Management And Prevention Of Diabetes Mellitus, Ashwini Wagle

Open Educational Resources

The Carbohydrate Counting tool was created to address the specific dietary needs of the Filipino population. It is an original intellectual work by Ashwini Wagle, Ed.D., MS, RD, Chair of the Department of Nutrition, Food Science, and Packaging at San Jose State University (SJSU).

The development of this tool was made possible through the collaboration and support of several contributors. Serena Starr, MS; Justine Martinez Cuaresma, MS, RD; Nazli Goudarzi, MS; Leah Olaivar; and Joe Johnston—a graduate student in the Department—played integral roles in the project. Their contributions included verifying the authenticity of traditional foods, designing culturally appropriate meal plans, …

Artificial Intelligence In Cancer-Related Malnutrition And Cachexia: A Transformative Tool In Clinical Nutrition, Salvatore Carbone

EVMS School of Health Professions Faculty Publications

[Introduction] Malnutrition and cachexia are common complications in cancer patients, and they negatively influence prognosis, treatment efficacy, and tolerability as well as quality of life [[1], [2], [3]]. Accurately identifying and effectively managing malnutrition and cachexia in this population remains a clinical challenge. Conventional validated screening tools may lack the sensitivity and specificity required for early detection and personalized intervention in diverse cancer types and treatment settings [4,5]. Over the last decade, the use of artificial intelligence (AI), including machine learning (ML) and deep learning (DL) strategies, has shown promising results in clinical nutrition, with the potential to revolutionize nutritional …

“What’S Supp” Developing An In Vitro Model For Healthy Oral Biofilms, Alex Labossiere

Open Access Dissertations

The study of oral plaque has largely been influenced by the study of pathogens. Here in Chapter 1, I will discuss how this pathogenic focus has been dominant until the onset of microbiome research and begin to explain microbiomes in the context of supragingival plaque in homeostatic communities. From there we will then discuss the possible roots where dysbiosis may arise from plaque to cause disease and ways in which we can study healthy supragingival plaque by in vitro models.

In chapter 2, I will discuss molecular commensalism and how it can be viewed as a tool to further understand …

Impact Of Whey And Soy Protein Supplementation On Resistance Training In Young Adults: A Systematic Literature Review And Meta-Analysis, Brandon Eric Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Whey protein is a popular supplement in sports nutrition. Soy protein is an alternative to whey protein, often marketed to individuals following a plant-based diet. Evaluating whey and soy protein supplementation's role in resistance exercise is essential for providing evidence-based nutrition guidance.

Objective: The study aim was to conduct a systematic literature review and meta-analysis on randomized control trials (RCTs) on the effectiveness of whey and soy protein supplementation on outcomes of lean body mass (LBM), squat, bench press, and plasma essential plasma amino acid (EAA) levels in young adult men and women.

Methods: The databases …

Beyond The Byproduct: Exploring Approaches For The Sustainable Valorization Of Underutilized Food Streams, Ian Phillip Israelsen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Commercial food processing creates side-streams that are often underutilized yet hold significant potential for valorization into value-added products for consumer use. This study investigated and compared potential protein recovery and lipid removal potential from buckwheat (BW; Fagopyrum esculentum Moench), brewer’s spent grain (BSG), and coffee silverskin (CSS) using a one-step solvent defatting and a pH-shift protein extraction method. The extraction efficiency yields of extracted protein from CSS were significantly lower than all other samples (p < 0.05). The defatted BW had the highest extracted protein content of all samples (p < 0.05). The sizes of the proteins in the starting materials were observed using LDS-PAGE. These sizes were consistent with published values and were mostly unchanged by defatting or the pH-shift process. The presence of 13S globulins and 2S albumins was confirmed under reducing conditions. Protein extracted from all native samples had lower total free essential amino acids than the starting materials. Native CSS and extracted protein followed this trend, but in defatted CSS and respective extracted protein samples, the extracted proteins had higher free essential amino acid content than the starting materials, sometimes of twofold or greater (p < 0.05). After defatting, the percentage of lipids recovered compared to the original lipid content of the samples was measured. The crude fat content of native and hexane-defatted BW flour was higher than BW flour defatted with ethanol (p < 0.05). The lipid extraction efficiency of all samples ranged from 4.6 ± 1.26%–43.20 ± 14.21%. The mass of the native and defatted starting materials were recorded at each step of processing, with reasonable losses assessed. The major non-polar lipid classes in each starting material were separated using thin-layer chromatography. All samples also displayed the presence of polar lipids. When comparing starting materials, BW and BSG was the most effective for protein extraction, and BSG has the most promise for lipid removal and potential for upcycling. All the examined starting materials show promise for use in food supplementation, especially as native flours. These products are plentiful and considered underutilized or side-stream products.

Molecular Characterization Of Fungus-Arthropod Associations, Angie M. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Arthropods, or phylum Arthropoda, are the largest group of animals, accounting for over three quarters of all animal records on NCBI Taxonomy. And yet, only 40% of all deposited sequences on NCBI Nucleotide come from arthropods. Clearly, arthropods are an understudied group of animals: they are small, often drab, and lead lives very alien to our own. Arthropods have chitinous exoskeletons, segmented bodies, and jointed appendages, and include crabs, barnacles, insects, spiders, and millipedes. Since the era of DNA based studies took off in the 1980s, DNA and other small molecules have been integral to our understanding of the Earth …

Evaluating Osmia Cornifrons As An Effective Pollinator For Agricultural Crops: An Analysis Of Hair Morphology And Sex-Ratio Distorting Bacterium, Wolbachia, Nellie Heitzman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Effective pollination relies not only on the presence of pollinators but also on their morphological adaptations and reproductive biology. In this study, I investigated both the external hair morphology and the intracellular bacterial infection status of Osmia cornifrons, a solitary bee species increasingly used in orchard pollination due to its efficiency and early seasonal activity. Using Scanning Electron Microscopy (SEM), I characterized the distribution and structure of two major hair types present on the bee's body: branched and unbranched. Branched hairs were found widely distributed across the head, thorax, dorsal abdomen, and legs, and appeared to function primarily in incidental …

Longitudinal And Ensemble Modeling Of Beef Cattle Feed And Water Intakes, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beef cattle production remains a cornerstone of global food systems, providing highly bioavailable protein and essential micronutrients that are difficult to secure from plant-based foods alone. Yet this production is also resource-intensive, requiring substantial land, feed, and water inputs while contributing significantly to greenhouse gas emissions. Efficiency of dry matter intake (DMI) and water intake (WI) is central to both productivity and sustainability, but accurate measurement of these traits—particularly in grazing systems—remains a major limitation. National WI standards continue to rely on pen-level data collected in the 1950s, while most existing DMI prediction equations perform poorly when applied beyond the …

Microbial And Plant Biodiversity In Appalachian Agroecosystems And Their Implications For Soil Health, Kinsey Reed Close

Graduate Theses, Dissertations, and Problem Reports (ETD)

The sustainability of agricultural landscapes depends on understanding the complex interactions between plants, soil, and microbes. In Appalachia, a region shaped by complex terrain and long histories of human and ecological interaction, small family farms produce hay, livestock, and row crops. These agroecosystems, though often treated as “artificial” by many ecologists, have the potential to support both food production and biodiversity conservation if managed with an understanding of ecological complexity. This dissertation examines how plant and microbial diversity interact with soil and management to influence nutrient cycling, soil health, and long-term resilience in Appalachian agricultural systems. A literature review revealed …

Characterization Of The Slc7a5/Slc3a2 Amino Acid Transporter In Human Pleural Mesothelial Cells, Ann Francis

Biotechnology Theses

The SLC7A5/SLC3A2 amino acid transporter moves essential amino acids, such as leucine, phenylalanine, and tryptophan, across the cellular plasma membrane. SLC7A5 overexpression was identified in diverse cancers, suggesting a role in tumorigenesis and cancer cell proliferation. However, the role of SLC7A5/SLC3A2 in other aggressive cellular phenotypes, including mesothelial mesenchymal transition (MesoMT), remains unexplored. RNA sequencing analyses identified SLC7A5 as one of three highly upregulated SLCs in mesothelial cell derived myofibroblasts. This sodium independent transporter exhibits a high affinity for branched chain amino acids such as leucine; however, what role, if any, this channel plays in MesoMT is unclear. SLC7A5 knockdown …