Digital Commons Network^™

Effect Of Chronic Alcohol Feeding Using The Lieber-Decarli Diet On Alzheimer’S Disease Pathology In Tg2576 Mice, Devaraj V. Chandrashekar, Nataraj Jagadeesan, Tamara Abdullah, Rudy Chang, Ross A. Steinberg, Frankey Sanchez, Elias Khal, Joshua Yang, David H. Cribbs, Derick Han, Rachita K. Sumbria

Pharmacy Faculty Articles and Research

Background: Chronic alcohol drinking is a modifiable risk factor for Alzheimer’s disease (AD), but underlying mechanisms remain poorly understood. Most studies of alcohol feeding to AD mice have utilized young mice and delivered alcohol in drinking water without controlling nutritional intake.

Objective: To study the impact of Lieber-DeCarli (LDC) liquid alcohol diet, which balances nutritional intake, on AD pathology of aged Tg2576 and wild-type (WT) mice, which is unexplored.

Methods: 13-month-old male and female Tg2576 or WT mice were fed LDC diet (5% ethanol or control) for six weeks (n = 11-13/group). Exploration (open-field test) and spatial reference memory …

Multi-Sensor Integration And Machine Learning For High-Resolution Classification Of Herbivore Foraging Behavior, Bashiri Iddy Muzzo, Kelvyn Bladen, Andres Perea, Shelemia Nyamuryekung’E, Juan J. Villalba

Wildland Resources Student Research

This study classified cows’ foraging behaviors using machine learning (ML) models evaluated through random test split (RTS) and cross-validation (CV) data partition methods. Models included Perceptron, Logistic Regression, Support Vector Machine, KNearest Neighbors, Random Forest (RF), and XGBoost (XGB). These models classified activity states (active vs. static), foraging behaviors (grazing (GR), resting (RE), walking (W), ruminating (RU)), posture states (standing up (SU) vs. lying down (LD)), and posture combinations with rumination and resting behaviors (RU_SU, RU_LD, RE_SU, and RE_LD). XGB achieved the highest accuracy for state classification (74.5% RTS, 74.2% CV) and foraging behavior (69.4% CV). RF outperformed XGB in …

Cardiometabolic Risk Factors Associated With Physical Fitness And Activity Levels: An Exploratory Study Of Us College Students, Keegan T. Peterson, Gabrielle Barraco, Jennifer Niessner, Melissa Rodgers, Melissa Bopp

International Journal of Exercise Science

International Journal of Exercise Science 18(8): 379-393, 2025. College students are a vulnerable population at risk of developing and/or experiencing poor physical fitness and insufficient physical activity (PA) levels, both associated with poor cardiometabolic health. Thus, this study assessed the association of physical fitness and domain-specific PA levels on cardiometabolic risk factors (CMRFs) among college students. A volunteer sample of students enrolled in general health and wellness courses at a large, Northeastern U.S. institution from Spring 2023 – Spring 2024 (n=1418, 69.0% male, 89.9% non-Hispanic White) completed an objective health assessment as part of a required course assignment. CMRFs (e.g., …

Diverse Roles Of Stress-Responsive Rnp Granules In Oogenesis And Infertility, M. Rebecca Glineburg, Carolee Nguyen

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Effectively responding to cellular stress (e.g., nutrient deprivation, oxidative stress) is essential for cell and organismal survival. A protective mechanism is especially critical in developing oocytes, where a prolonged quiescent state and the inability to divide render oocytes highly susceptible to accumulating stress that can result in cell death if unaddressed. Despite the common view that stress granules are the primary stress-responsive ribonucleoprotein granule, accumulating evidence shows that in ovaries, other ribonucleoprotein granules also uniquely mediate gene regulation in response to stress. Here, we review recent insights into ribonucleoprotein granule dynamics and ribonucleoprotein granule protein function during stress in the …

Genome And Gwas Analyses For Soybean Cyst Nematode Resistance In Usda World-Wide Common Bean (Phaseolus Vulgaris) Germplasm, Ainong Shi, Haizheng Xiong, Thomas E. Michaels, Senyu Chen

Horticulture Faculty Publications and Presentations

Soybean cyst nematode (SCN), Heterodera glycines, has become a significant threat in common bean (Phaseolus vulgaris) production, particularly in regions like the upper Midwest USA. Host genetic resistance offers an effective and environmentally friendly approach to managing SCN. This study aimed to conduct a genome-wide association study (GWAS) and genomic prediction for resistance to SCN HG Types 7 (race 6), 2.5.7 (race 5), and 1.3.6.7 (race 14) using 0.7 million whole-genome resequencing-generated SNPs in 354 USDA worldwide common bean germplasm accessions. Among these, 26 lines exhibited resistance to all three HG types, with a female index (FI) of less than …

Neighborhood Disadvantage And Inequities In Access To Preemptive And Living Kidney Transplantation, Brandon M Fairless, Oluwatunmise A Fawole, Duc T Nguyen, Ankona Banerjee, Kenneth J Nobleza, Abiodun O Oluyomi, Omar Rosales, Jayna M Dave, Elizabeth A Onugha

Children’s Nutrition Research Center Staff Publications

Key Points:

1. Pediatric patients residing in low-resource neighborhoods experienced lower rates of preemptive and living donor kidney transplantation.

2. Household-level factors (single-parent, non-English primary language, and receiving government assistance) may also affect the type of transplant received.

3. Area Deprivation Index scores offer an objective way to quantify neighborhood-level disadvantage and can enhance pretransplant screening.

Background: Living donor kidney transplant (LDKT) generally results in better outcomes than deceased donor kidney transplant (DDKT). Preemptive kidney transplant (KT) allows patients to bypass undergoing maintenance dialysis and is associated with improved patient and graft survival. Studies in the US pediatric population have shown racial-ethnic disparities …

Molecular Mechanisms And Role Of Camel Milk Derived Bioactive Peptides Towards Insulin And Glp-1 Receptor Activation, Irfa Anwar

Thesis/ Dissertation Defenses

Camel milk (CM), renowned for its exceptional nutritional and medicinal properties, has been a crucial resource in arid regions for centuries. Its notable antidiabetic effects, supported by extensive research through in vitro, in vivo, and clinical studies, have gained significant scientific attention. However, the underlying cellular and molecular mechanisms remain largely unclear. Our previous research demonstrated that camel milk whey hydrolysates (CWHs) induce insulin receptor (IR) activation and downstream signaling, promoting glucose uptake in vitro using HEK293 and HepG2 cells. Building on this foundation, the current study focused on synthesizing nine of the most potent peptides (designated P1-9) derived from …

Variation In Response To Phosphorus Among Sweetpotato (Ipomoea Batatas L.) Cultivars, Jeffrey Cole Denis Gregorie

LSU Doctoral Dissertations

Phosphorus (P) is essential for plant growth and is often a major limiting nutrient in agriculture. In sweetpotato (Ipomoea batatas), studies on the role of P on storage root yield have been limited because of the lack of response and inconsistent results across cultivars and years. Growing evidence shows that current phosphate (Pi) fertilizer rates can be reduced while maintaining productivity. Newly published molecular evidence shows differential expression of phosphate starvation response genes among sweetpotato cultivars, further supporting the hypothesis of cultivar-specific Pi sensitivity. The research herein sought to characterize root system architecture and yield response to Pi …

Adipose Progenitor Cell-Derived Extracellular Vesicles Suppress Macrophage M1 Program To Alleviate Midlife Obesity, Qing Zhou, Jia Gao, Guorao Wu, Chenwei Wang, Yan Yang, Teng Huang, Yi Wang, Tiantian Yue, Zhichao Gao, Hao Xie, Fei Xiong, Ke Xiang, Tuying Yong, Wanguang Zhang, Tongtong Zhang, Wen Kong, Cai Chen, Shu Zhang, Qilin Yu, Xuemei Fan, Shiwei Liu, Yanjun Liu, Cong-Yi Wang

Children’s Nutrition Research Center Staff Publications

Among different age groups, middle-aged individuals are particularly susceptible to obesity, with a 22% higher risk of all-cause mortality. However, the underlying mechanisms remain unclear. In this study, we identify adipose progenitor cells (APCs) in the white adipose tissue (WAT) of middle-aged subjects as potential causes of midlife obesity. Specifically, the extracellular vesicles (EVs) derived from APCs display an impaired ability to mitigate the inflammaging of adipose tissue macrophages (ATMs) in middle-aged individuals. Mechanistically, these EVs, lacking miR-145-5p, fail to suppress the expression of L-selectin in ATMs, thereby facilitating their M1 program via the NF-κB signaling pathway. In contrast, EVs …

Integrating E-Commerce To Reduce Postharvest Losses In Rural Cameroon, Maurice Tchasso

Walden Dissertations and Doctoral Studies

E-commerce has the potential of assisting the agriculture market to curb postharvest losses by connecting new and existing customers to agricultural products more efficiently. The purpose of this qualitative phenomenological study was to explore and describe the lived experiences of successful farmers in rural Cameroon to obtain insights into the e-commerce factors and strategies they use to manage and reduce postharvest losses. The study sample was 20 rural Cameroonian farmers using e-commerce in reducing postharvest losses. The conceptual framework for this study was Rogers’s diffusion of innovation theory. The data collection occurred through virtual interviews with the participants and their …

Growing The Future: Community Gardens In Hillsborough County, Florida, Nicole M. Kennady

USF Tampa Graduate Theses and Dissertations

This project was an evaluation on community gardens in Hillsborough County, Florida from May 2024 to November 2024, built off of Homegrown Hillsborough’s first year evaluation of the current Hillsborough County food system. Homegrown Hillsborough is a network of community members with a mission to grow a strong food system in Hillsborough County by assisting in all areas of the food system from food production to food waste. Homegrown Hillsborough plans to act as a development initiative focused on community nutrition. Over the next two years, Homegrown Hillsborough plans to focus on food production, food access, and economic development. Through …

Adiporon, Adiponectin Receptor Agonist, Exhibits Antiproliferative And Apoptotic Effects In Ht29 And Hct116 Colon Cancer Cells, Arwa Fairaq, Ashraf N. Abdalla, Alanood S. Algarni, Yosra Alhindi

Saudi Toxicology Journal

AdipoRon is the first synthetic analog of endogenous adiponectin, which is an adipose tissue derived hormone. As it is characterized with pharmacological properties like adiponectin through binding and activating AdipoR1 and AdipoR2 receptors this makes it a target for therapeutic treatments for a multitude of disorders. In this study AdipoRon effect on HT29 and HCT116 colon cancer cells were investigated. Cytotoxic activity of AdipoRon was observed, with a significant inhibition rate in HT29 and HCT116 cells. Moreover, there was a significant decrease in the number of HCT116 colonies by clonogenicity test. In addition, cell migration assay showed significant decrease in …

Emerging Clostridioides Difficile Ribotypes Have Divergent Metabolic Phenotypes, Firas S Midani, Heather A Danhof, Nathanael Mathew, Colleen K Ardis, Kevin W Garey, Jennifer K Spinler, Robert A Britton

Faculty, Staff and Students Publications

Clostridioides difficile is a gram-positive spore-forming pathogen that commonly causes diarrheal infections in the developed world. Although C. difficile is a genetically diverse species, certain ribotypes are overrepresented in human infections, and it is unclear if metabolic adaptations are essential for the emergence of these epidemic ribotypes. To identify ribotype-specific metabolic differences, we therefore tested carbon substrate utilization by 88 C. difficile isolates and looked for differences in growth between 22 ribotypes. As expected, C. difficile was capable of growing on a variety of carbon substrates. Further, C. difficile strains clustered by phylogenetic relationship and displayed ribotype-specific and clade-specific …

Exploring Profiles Of Fathers Integrating Food And Physical Activity Parenting Practices, John A Jimenez-Garcia, Louise C Mâsse, Robert L Newton, Salma M Musaad, Alicia Beltran, Teresia M O'Connor

Children’s Nutrition Research Center Staff Publications

Objective: This study aims to identify fathers' profiles integrating food parenting practices (FPP) and physical activity parenting practices (PAPP).

Design: We analysed cross-sectional data. The fathers completed the reduced FPP and PAPP item banks and socio-demographic and family dynamics (co-parenting and household responsibility) questionnaires. We identified fathers' profiles via latent profile analysis. We explored the influence of social determinants, child characteristics and family dynamics on fathers' profiles using multinomial logistic regression.

Setting: Online survey in the USA.

Participants: Fathers of 5-11-year-old children.

Results: We analysed data from 606 fathers (age = 38 ± 8·0; Hispanic = 37·5 %). Most fathers …

Genetic Associations With C-Peptide Levels Before Type 1 Diabetes Diagnosis In At-Risk Relatives, Taylor M Triolo, Hemang M Parikh, Mustafa Tosur, Lauric A Ferrat, Lu You, Peter A Gottlieb, Richard A Oram, Suna Onengut-Gumuscu, Jeffrey P Krischer, Stephen S Rich, Andrea K Steck, Maria J Redondo

Children’s Nutrition Research Center Staff Publications

Objective: We sought to determine whether the type 1 diabetes genetic risk score-2 (T1D-GRS2) and single nucleotide polymorphisms are associated with C-peptide preservation before type 1 diabetes diagnosis.

Methods: We conducted a retrospective analysis of 713 autoantibody-positive participants who developed type 1 diabetes in the TrialNet Pathway to Prevention Study who had T1DExomeChip data. We evaluated the relationships of 16 known single nucleotide polymorphisms and T1D-GRS2 with area under the curve (AUC) C-peptide levels during oral glucose tolerance tests conducted in the 9 months before diagnosis.

Results: Higher T1D-GRS2 was associated with lower C-peptide AUC in the 9 months before …

The Perceived Benefits Of And Barriers To Open-Water Swimming: A Mixed-Methods Examination, David Elliott Ph.D, Mark A. Christie Ph.D

International Journal of Aquatic Research and Education

This study set out to determine the perceived benefits of, and barriers to, open-water swimming (OWS). Adopting a mixed-methods approach, six hundred and sixty-five open-water swimmers responded to an online survey consisting of the Exercise Benefits and Barriers Scale (EBBS) and a series of open-ended questions. Data from the EBBS revealed the strongest perceived benefits to be ‘Psychological Outlook’ and ‘Life Enhancement.’ In terms of barriers, those presented in the EBBS received low ratings with only ‘Physical Exertion’ considered as being somewhat problematic. Content analysis performed on the open-ended responses showed social aspects, mental well-being, and connection to nature as …

Inflammation And Gut Barrier Function-Related Genes And Colorectal Cancer Risk In Western European Populations, Hannah B Mandle, Mazda Jenab, Marc J Gunter, Anne Tjønneland, Anja Olsen, Christina C Dahm, Jie Zhang, Pierre-Emmanuel Sugier, Joseph Rothwell, Gianluca Severi, Rudolf Kaaks, Verena A Katzke, Matthias B Schulze, Giovanna Masala, Sabina Sieri, Salvatore Panico, Carlotta Sacerdote, Catalina Bonet, Maria-Jose Sánchez, Pilar Amiano, José María Huerta, Marcela Guevara, Richard Palmqvist, Thyra Löwenmark, Aurora Perez-Cornago, Elisabete Weiderpass, Alicia K Heath, Amanda J Cross, Paolo Vineis, David J Hughes, Veronika Fedirko

Faculty, Staff and Student Publications

Gut barrier dysfunction and related inflammation are known to be associated with the development and progression of colorectal cancer (CRC). We investigated associations of 292 single-nucleotide polymorphisms (SNPs) from 27 genes related to endotoxins/lipopolysaccharide (LPS) sensing and tolerance, mucin synthesis, inflammation, and Crohn's disease with colon and rectal cancer risks. Incident CRC cases (N = 1374; colon = 871, rectum = 503) were matched 1:1 to controls nested within the European Prospective Investigation into Cancer and Nutrition cohort. Previously measured serum concentrations of gut barrier function and inflammation biomarkers (flagellin/LPS-specific immunoglobulins and C-reactive protein [CRP]) were available for a sub-set …

The Spice (Sustainable, Physics-Inspired Culinary Education) Lab – A Digestible Few-Nexus Educational Platform, Carla Ramsdell

National Collaborative for Research on Food, Energy, and Water Education (NC-FEW)

The SPICE (Sustainable, Physics-Inspired Culinary Education) Lab is a unique educational platform that enables participants from a wide disciplinary background to engage and become literate in the unique connections between food, energy and water. This small but mighty space allows for outreach education in many formats, including formal college courses, university-centered hands-on activities and public outreach.

The work performed in this space fills a unique need in the FEW-Nexus education opportunities because it is adaptable to many learners and learning opportunities and its focus on kitchen science makes these concepts approachable which can later be expanded to understand other critical …

Addressing Local Food Insecurity Through A Problem-Based Learning Classroom, Kaley Mumma, Aimable Mugabo

National Collaborative for Research on Food, Energy, and Water Education (NC-FEW)

Through the frame of Problem-Based Learning (PBL), a curriculum is proposed to engage students in addressing local food insecurity. Students collaboratively define the problem, explore underlying issues, establish goals, design solutions, and implement their chosen solution to address food insecurity within their community. The importance of community engagement and utilizing local resources are highlighted in advising the implementation of this program.

Integrating Food, Water, And Agriculture Education In Early Childhood: A Baseline Study Of Farm To Ece Programming, Kyle Whitley, Kelsey Hall

National Collaborative for Research on Food, Energy, and Water Education (NC-FEW)

This baseline study examined how [State] early care and education (ECE) providers implemented activities and access to locally grown foods, gardening, and learning about food, nutrition, water use, composting, and agriculture. Over the past three years, 254 respondents participated in farm to ECE, with many providers cooking or holding taste tests, doing agriculture/nutrition education, and conducting field trips. Universities, farmers, community organizations, and Extension agents could alleviate the primary barriers and support ECE providers through curriculum, website content, or trainings on how to teach 3-5 year olds agriculture, gardening, plant life cycle, water, the environment, or composting.

Physical And Biological Effects On Moths’ Navigation Performance, Yiftach Golov, Roi Gurka, Alexander Liberzon, Ally Harari

Physics and Engineering Science

In a chemosensing system, the local olfactory environment experienced by a foraging organism is defined as an odorscape. Using the nocturnal pink bollworm moth (Pectinophora gossypiella), we tested the combined effect of three biophysical aspects in its immediate odorscape to shed light on the coupling effects of biotic and abiotic factors on navigation performances of a nocturnal forager: i) the quality of the pheromone source, ii) the pheromone availability, and iii) the airflow characteristics. The navigation performance of the males was investigated using a wind tunnel assay equipped with 3D infrared high-speed cameras. The navigation performance of the males was …

The Sustainability Stones: Culturally Embedded Conservation Strategies And Their Vulnerability In Maupiti, French Polynesia, Russell Fielding, Fiona Gimenez

Honors College Faculty Publications

This paper explores culturally embedded conservation strategies through the lens of the traditional agroforestry and fisheries cycles in Maupiti, French Polynesia. By pairing certain breadfruit cultivars with specific fish species, the island’s community created a culinary system that regulates the seasonal consumption of marine resources, making the sustainable use of those resources more likely. While modern pressures such as reduced breadfruit diversity, competition with imported foods, and climate change have weakened these traditional practices, they remain an example of local ecological knowledge guiding conservation. The study highlights the threat of losing sustainable resource-use practices and biodiversity as both biodiversity and …

Precision Phenotyping For Curating Research Cohorts Of Patients With Unexplained Post-Acute Sequelae Of Covid-19, Alaleh Azhir, Jonas Hügel, Jiazi Tian, Jingya Cheng, Ingrid V Bassett, Douglas S Bell, Elmer V Bernstam, Maha R Farhat, Darren W Henderson, Emily S Lau, Michele Morris, Yevgeniy R Semenov, Virginia A Triant, Shyam Visweswaran, Zachary H Strasser, Jeffrey G Klann, Shawn N Murphy, Hossein Estiri

Faculty, Staff and Student Publications

BACKGROUND: Scalable identification of patients with post-acute sequelae of COVID-19 (PASC) is challenging due to a lack of reproducible precision phenotyping algorithms, which has led to suboptimal accuracy, demographic biases, and underestimation of the PASC.

METHODS: In a retrospective case-control study, we developed a precision phenotyping algorithm for identifying cohorts of patients with PASC. We used longitudinal electronic health records data from over 295,000 patients from 14 hospitals and 20 community health centers in Massachusetts. The algorithm employs an attention mechanism to simultaneously exclude sequelae that prior conditions can explain and include infection-associated chronic conditions. We performed independent chart reviews …

More Choosy For Minor Foods: Diet, Seasonality And Food Selection In Sympatric Frugivorous And Folivorous Lemurs, Mitchell Irwin, Vololonirina Rahalinarivo, Bruno Ramorasata, Jean-Luc Raharison, Jean-Freddy Ranaivoarisoa, Chloé Gherardi, Jessica Rothman

Faculty Articles, Papers, and Other Scholarship

Despite traditional dietary categories common in primatology (folivore, frugivore, insectivore), most primates use one or more food types beyond their primary one. Among lemurs, folivores tend to have a complicated, long gastrointestinal tract with an enlarged cecum, shearing teeth, and microbiome adaptations to foliage, while frugivores have simple teeth and guts and fast gut passage. Yet frugivores often eat some leaves, and folivores eat some fruit, and little is known about the selection rules they follow within each food type. We quantified diet and food chemistry for two sympatric rainforest lemurs: a cathemeral frugivore (brown lemur, Eulemur fulvus) and a …

Analysis Of The Effect On N Nutrition On Dry Matter Yield Of A Sward By Reference To Potential Yield And Optimum N Content, G Lemaire, F Gastal, J Salette

IGC Proceedings (1977-2023)

Previous work has shown that the N content of a sward could be related to the dry matter accumulation during the course of the regrowth by the following equation : N % = a(Wtb, where W is the weight of aboveground biomass and N % the N concentration of dry matter (Salette & Lemaire, 1981). The coefficient a represents the N % for an aerial biomass of I t/ha, and b characterizes the degree of dilution of N during the regrowth. When b = 0, N % remains constant during the regrowth. If b = I, there is an increase …

Alimentation Minerale, Niveau Hydrique Et Utilization De L’Eau Par La Luzerne, Micheline Anneessens

IGC Proceedings (1977-2023)

L'eau est souvent un facteur limitant !es rendements puisque des problemes se posent au niveau de sa repartition dans l'es­pace et dans le temps. Aucune etude n'a encore montre comment dose et composi­tion d'engrais interagissent avec l'humidite du substrat sur ]'utilisation de l'eau par une plante fourragere forte consomma­trice de cette ressource.C'est dans ce but que le present essai a ete realise sur la luzerne.

Optimization Of Grassland Ecosystem Production By Anthropogenic Treatment, R Holubek, J Jancovic

IGC Proceedings (1977-2023)

Abundant amount of the new knowledge was gained by an intensive research of grassland ecosystem in Czechoslovakia and other European countries during last 25 years. It was con­firmed that under favourable humid conditions and sufficient macronutrients and micronutrients together with adequate N­fertilizing should be nearly reached ecologically conditioned level of potential productivity and dry matter or N-compounds yields should be increased by 100-200 or 200-300 per cent respectively (Velich, 1986). Introduction of scientific methods of controlled agricultural production together with higher landscape protection requirements, stability of ecosystems, quality and health­proved foods requirements have changed the traditional views to the nutrition …

Experiment Of Trace Elements Fertilizer Spraying On Sown Pasture, Tu Minyi

IGC Proceedings (1977-2023)

The application of compound phosphate in the way of rot­ationary fertilizing in sections in successive years kept the vig­orous growth of pasture composed of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) in the first several years after the pasture was grazed in 1981. But the pas­ture could not keep the growing vigour even with the same level of fertilization in the last two years. Considering the unba­lanced nutrition being one of the major reasons, a preliminary experiment of trace elements spraying has been undertaken in Nanshan Sown Pasture-land aiming at identifying if trace fert­ilizers …

Some Nitrogen Forms And Micronutrients In Silage Maize Leaves As Affected By Nk Fertilization And Liming, C.L F. Carranca, M A. Castelo Branco

IGC Proceedings (1977-2023)

The nutritive value of a forage depends on the quality which, in time, is dependent on protein, sugar, cellulose, fat and min­eral elements content. The existence of deficiencies may limit the maximum yield and also the quality. Several experiments have been carried out in order to study the effect of liming and NK fertilization on the nutrients uptake by silage maize and yields.

The Diagnosis Of Grassland Mineral Nutrient Status Through Herbage Analysis, J Salette, Lydie Huche

IGC Proceedings (1977-2023)

It appears more and more clearly that soil analysis is not sufficient to give a good approximation of the mineral status of grassland soils. In perennial crops leaf analysis and empirical critical levels are widely utilized. This grassland approach emphasizes the good results which can be obtained for nitro­gen, potassium, phosphorus not from leaf but from the whole herbage plant analysis. This paper deals with pure grass stands.