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Evaluation Of Performance Characteristics And Groundwater Contamination Risks Associated With On-Farm Swine Carcass Disposal Via Composting And Shallow Burial With Carbon, Gustavo Castro Garcia

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation evaluates the performance of three on-farm swine carcass disposal methods: whole carcass composting (WCC), ground carcass composting (GCC), and shallow burial with carbon (SBC), focusing on biosecurity factors and groundwater contamination risks. Foreign animal diseases (FADs), such as African swine fever and classical swine fever, pose severe economic and animal well-being risks if introduced to the United States. With confirmation of an FAD, swine movement will be halted, creating an urgent need for practical, biosecure, and environmentally responsible on-farm disposal strategies for swine carcasses. Nebraska, as a leading swine-producing state, exemplifies the vulnerability of high-density livestock regions to …

Educating The New Generation Of Food Scientists Through Innovative Learning Experiences, Karen Andrea Nieto Flores

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Food science provides an opportunity to engage high school students in Science, Technology, Engineering, and Mathematics (STEM) by connecting classroom concepts to real-world applications. Despite the field’s relevance to public health, food safety, sustainability, and innovation, food science remains largely absent from high school curricula, and few students recognize it as a viable STEM pathway. This research addressed this gap by evaluating immersive summer camp experiences and teacher-focused curriculum review as strategies to increase food science awareness and interest among students and educators. A literature review traced the historical development and current landscape of K–12 food science education, identifying barriers …

Egg White Hydrolysate In Hypertension: A Mechanistic Investigation Of Gut Microbiota Dependent And Independent Pathways, Emerson David Nolasco

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cardiovascular diseases are one of the leading causes of mortality worldwide in which hypertension is a risk factor. Present pharmacological interventions require long-term adherence to the therapy and are often associated with undesirable side effects. Moreover, treatments have overlooked the role of the microbiome in the development of hypertension. Egg white hydrolysate (EWH) has exhibited antihypertensive properties with potential to be used as a natural alternative for the prevention and management of hypertension. However, EWH peptide’s effect on the gut microbiome remains yet to be elucidated. Studies have described that a portion of the ingested protein can remain undigested as …

B.R. Wells Arkansas Rice Research Studies 2024, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Research reports contained in the publication may represent preliminary or only a single year of results; therefore, these results should not be read as a basis for long-term recommendations.

Several research reports in this publication will appear in other University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas rice producers of all the research being conducted with funds from the rice check-off program. This publication also contains research funded by industry, federal, and state agencies.

Use of products …

Trophic Roles And Climate-Driven Vulnerability For Benthic Invertebrates At Artificial Reefs In The Northern Gulf Of Mexico, Amanda Kirkland

LSU New Orleans Theses and Dissertations

Artificial reefs are deployed to enhance marine habitats and fisheries, yet their role in supporting secondary production and their vulnerability to climate stressors remain understudied. This dissertation investigates the trophic ecology and climate sensitivity of benthic invertebrates at artificial reefs in the northern Gulf of Mexico. Chapter 1 assessed reef food webs using stable isotopes from fishes, invertebrates, and primary producers across six reefs and two seasons. Mixing models showed that most fishes rely on benthic reef carbon, while suspension feeders consume plankton and sediment microphytobenthos. Isotopic niche analysis revealed functional diversity and seasonal shifts in δ¹³C and δ³⁴S, though …

Breeding Biofortified Protein Rich Organic Pulses For Better Human Health, Sonia Salaria

All Dissertations

Pulse crops are dry grains of legumes, the second most consumed plant crops in the world after cereals. These crops are one of the primary sources of plant-based protein and contain high concentrations of pre-biotic carbohydrates, minerals and vitamins. Recognizing the health benefits of pulses intake, their demands as sustainable food and changes in people’s diet preferences for plant-based protein have increased awareness for pulses consumption. With this scenario, improving the nutritional profile of pulse crops for rich nutrition has been an opportunity for breeders to explore and tailor the available germplasm to develop nutrient dense pulses. Furthermore, the genomic …

Alternative Therapeutics For Human Breast Cancer: Nutraceutical Underpinnings, In Vitro Metabolomic Profiling, Treatment Efficacy, And Contextual Ethical Implications, Aubrey A. Mattingly

All Dissertations

Breast cancer remains one of the most prevalent diseases worldwide, yet therapeutic equity is still lacking. Standard treatments including surgery, radiotherapy, chemotherapy, and long-term hormone therapy. These approaches are often accompanied by significant side effects. While newer methods such as immunotherapy and targeted therapies have emerged, chemotherapy protocols and incorporation have remained largely unchanged in the past decades. In this context, nutraceuticals represent an alternative or adjuvant to therapy worthy of exploration. The reviewed literature highlights the anti-carcinogenic activity in selected nutraceuticals, marjoram, thyme and persimmon, considering their phytochemical constituents and secondary metabolites. Metabolomic profiling using the Biolog Phenotype Mammalian …

Habitat Selection Analysis Of Mule Deer To Determine Efficacy Of The Coyote Bounty Program In Utah, Xaela M. Walden

All Graduate Theses and Dissertations, Fall 2023 to Present

Mule deer populations have been declining over the past couple of decades in many parts of the western United States. Often times, predators are blamed for the decreases in deer populations. Because of this, wildlife managers implement programs focused on reducing the number of predators on the landscape in an attempt to increase deer populations. However, predator removal programs have had mixed results, thus further investigation is needed to determine if coyote removals by the public through a bounty program have a positive effect on mule deer populations. My study investigated whether coyote removals through the bounty program in Utah …

Acute Exposure Of American Oyster (Crassostrea Virginica) To Selected Antidepressant Drug: Histological, Biochemical, And Immunohistochemical Assessments, Md. Faisal Amin

Theses and Dissertations

The rapid increase in pharmaceutical use has brought significant health benefits to humans. Studies have shown that many pharmaceuticals, such as antidepressants, are often detected in surface waters, groundwater, and even drinking water. These pharmaceuticals in aquatic environments threaten marine life seriously. Marine bivalves are vital source of seafood for people worldwide. In this study, we examined the effects of short-term exposure to fluoxetine, an antidepressant used to treat depression, at low (0.5 μg/L) and high (5 μg/L) doses over a one-week period on American oysters (Crassostrea virginica) under controlled laboratory conditions. Histological analysis revealed atrophy in the gills and …

Fluoride Toxicity And Benefit: An Integrated Review Of Mechanisms, Risks, And Public Health Impact, Sophia Chavarria

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Fluoride has long been praised for its role in preventing dental caries, primarily through its effects on enamel remineralization and antibacterial effects. However, growing evidence from molecular and cellular studies suggests that excessive fluoride exposure, especially during tooth development, can lead to dental fluorosis and systemic toxicity. This paper examines the biological mechanisms underlying fluoride-induced dental fluorosis, with a focus on stress responses in ameloblasts, oxidative damage, impaired autophagy, and disrupted protein processing. It integrates data from in vitro, in vivo, and human studies to evaluate the cellular thresholds at which fluoride shifts from therapeutic to toxic. Systemic risks, including …

A Simple Guide For Describing Soils, 2nd Edition, Angela Stuart-Street, Nicolyn Short Dr, Paul Galloway, Noel R. Schoknecht

Natural resources published reports

Soils are enormously diverse and can be very confusing to understand and talk about. This simple guide for describing soils helps to identify the most important parts of a soil profile and provide an easy way to understand and explain what you see. It gives you a step-by-step guide of what soil properties to describe and how to describe them, along with the tools to make basic soil classifications. The soil descriptors help you to identify the soil type and aid in assigning a simple and standardised name to the soil. While this guide is designed to link with a …

Cg18562578 Methylquant Assay For The Discrimination Of Monozygotic Twins, Amber P. Chauca

Student Theses

Monozygotic (MZ) twins have identical DNA, making traditional profiling methods insufficient in their discrimination. However, MZ twins can be differentiated by their levels of DNA methylation. Our research group previously developed a methodology to detect cg18562578 DNA methylation levels in MZ twins based on a two- step PCR approach and a cg18562578 MethylQuant Assay. That study generated data for only 64% of the samples it investigated, and showed a discrimination power of 77% to discriminate between MZ twins. Here, we used the same methodology but a different approach to determine DNA methylation in 73 previously unsuccessful twin pairs. We diluted …

Assessment Of Floral Resources From Three Tamaulipan Thornscrub Communities Of The Lower Rio Grande Valley, Gracie Makenna Granados

Theses and Dissertations

The Tamaulipan thornscrub is a transition zone between the neotropics and the temperate regions, harboring high biodiversity. This habitat supports a diverse insect community, including many pollinators of native and cultivated plants in Texas and northeastern Mexico. This ecosystem has been extensively degraded and lost; only 5% of its original cover remains north of the Rio Grande. Restoration of the Tamaulipan thornscrub has been ongoing for over three decades, but the important roles of pollinators in the success of such efforts and the functioning of the restored ecosystem have rarely been considered in restoration efforts. The purpose of this study …

Arginine Vasotocin And Corticosterone In Nesting And Non-Nesting Loggerhead Sea Turtles In X’Cacel-X’Cacelito Sanctuary, Krista Blair Reed

Theses and Dissertations

Sea turtles exhibit distinct behaviors before and after egg laying, however, not all individuals successfully oviposit. Some females display disruptions in their typical nesting behavior, returning to the water without laying eggs, which is referred to as a non-nesting emergence (NNE). NNEs can be due to external disturbances on the beach, but why they occur is often unclear. Arginine vasotocin (AVT) is a neuropeptide involved in various physiological processes, including stimulating smooth muscle contraction of the oviduct to facilitate egg deposition in nesting sea turtles. On the other hand, corticosterone is the primary stress hormone in sea turtles and is …

Exploring Transcriptional Stress Responses In Sorghum And Evolutionary Insights Into Yth Domain Proteins Across Plants, Shikha Bharti

Theses and Dissertations

Understanding how plants respond to abiotic stress is crucial for enhancing crop resilience in the face of climate change. This thesis explores plant stress adaptation mechanisms through two interlinked studies that address different aspects of gene regulation. The first study investigates the transcriptional dynamics of Sorghum bicolor under drought and heat stress at 1-hour and 6-hour time points. By employing 3'-end RNA sequencing, this study provides a comprehensive temporal view of gene expression changes, revealing stress-responsive transcription factors, hormone-associated pathways, and chromatin regulators. The comparative analysis highlights both common and distinct molecular responses between drought and heat stress, providing insights …

Analyzing How Taste And Nutrition Impact Learning And Memory In Honey Bees Through Olfactory Associative Conditioning, Alessandra Elizabeth Perry

Theses and Dissertations

Honey bees detect sweet nectar, but not all sweet-tasting sugars provide nutrition. Unlike fruit flies, which can learn to avoid non-nutritious sweet foods, bees may lack this ability. To test if bees form caloric frustration memory (CFM), we conditioned them using odors paired with L-glucose (sweet but non-nutritious) or D-glucose (sweet and nutritious). Bees rewarded with L-glucose showed lower learning and long-term memory compared to D-glucose. Bees cannot differentiate between an odor associated with rewards containing malaise-causing compounds and an odor associated with a reward alone during differential conditioning. Therefore, we used differential conditioning to determine if lower acquisition in …

Transcriptomic Study On The Effect Of Salt Stress In Sorghum Bicolor Seedlings, Joshwa A. Gandy

Theses and Dissertations

The global impact and reliance on cereal crops, and sorghum in particular, is paramount for worldwide food security and for the livelihood of hundreds of millions of people. The detrimental effects of salt stress on crop plants and the huge economic losses it causes by affecting agricultural output worldwide is a devastating issue with huge impacts, which have prompted research to combat salinity in numerous ways. Understanding how cereals respond to the negative effects of salt stress is crucial for enhancing their salt tolerance. In this study of sorghum, we used a 3’-end RNA sequencing approach to elucidate major salt …

Investigating The Multiple Enzyme Complex In The Carotenoid Biosynthesis Pathway, Mercy Mormor

Electronic Theses and Dissertations

In nature, plant carotenoids are 40-carbon isoprenoids with polyene chains that may have double bonds. They are vital components of all photosynthetic organisms, playing several roles in the plant cell, including photosynthesis, pigmentation, phytohormone synthesis, signaling and photoprotection. In this study, we investigated protein-protein interactions among key enzymes involved in the carotenoid biosynthesis pathway using the Bimolecular Fluorescent Complementation (BiFC) assay. Our results revealed several interactions that suggest a coordinated regulation of enzymatic activity within this pathway, highlighting potential regulatory complexes that influence carotenoid production. The study also highlights the pivotal role of Zeta-Carotene Isomerase (Z-ISO) as a key regulatory …

Gut Passage Time In Small Mammals: Influences Of Small Mammal Traits, Digesta Phase, And Particle Morphology, Noah G. Hale

Electronic Theses and Dissertations

Dispersal is crucial in maintaining healthy ecosystems, but little is known about mechanisms influencing fungal spore dispersal by animals. We aimed to develop a more mechanistic understanding of the influence of fungal spore traits and small mammal traits on gut retention time. We fed three species of small mammals (shrew, mouse, vole), which differ in gut morphologies, digestive strategies, and activity a combination of markers including: liquid digesta phase (CrEDTA), small particle (fungal spores with diverse morphologies), and large particles (glitter and beads, representing plant cells and seeds, respectively). We found that hindgut fermenters selectively retained the liquid markers, spores …

Performance And Feasibility Of Pasture Rangeland And Forage Insurance In The Delta Region, Walker Bradley Davis

Graduate Theses and Dissertations

Despite being available nationwide since 2016, enrollment in Pasture, Rangeland, and Forage (PRF) insurance remains low in many regions of the country. The program's performance also presents regional discrepancies, with loss ratios varying significantly. Studies that have simplified the complexity of interval selection for producers have been shown to positively affect PRF enrollment. Such studies remain limited in several forage-producing areas. This study aims to address the lack of information in Arkansas specifically. Arkansas has among the nation's lowest participation rates and loss ratios. Low loss ratios signal that participants pay more in premiums than received in indemnities and may …

Using The Dash Diet To Create Hypertensive-Friendly Dishes For People Of Chinese Heritage, Kady Li

Electronic Theses, Projects, and Dissertations

Cardiovascular disease is the leading cause of death worldwide, associated with high sodium intake being a primary contributor to elevated blood pressure. The US Dietary Guidelines for sodium intake is 2,300 mg per day; however, studies indicate that the average daily sodium consumption among Chinese adults is 5,013 mg. The Dietary Approaches to Stop Hypertension (DASH) diet is often recommended to those diagnosed with hypertension. This project aims to develop a cookbook featuring lower-sodium Chinese recipes following the DASH diet guidelines. Twenty-five traditional Chinese recipes were modified to align with DASH diet guidelines (25% reduction in sodium content). Nutrient analysis …

Investigating The Impact Of Psychoactive Plant Alkaloids On Bumblebee Behavior, Skylar Mathieson

Electronic Theses and Dissertations

The relationship between plants and their pollinators is often framed as a food-for-service exchange in which plants provide pollinators with nutrients in exchange for transport of their gametes. However, in addition to essential nutrients, many floral rewards (nectar, pollen) also contain defensive toxins such as alkaloids, with largely unexplored consequences for plant-pollinator interactions. I investigated how three psychoactive and addictive plant alkaloids — caffeine, nicotine, and morphine — affect foraging behavior in generalist bumblebees (Bombus impatiens) using robotic flowers. Each of these chemicals affected pollinator foraging behavior, influencing the number and duration of their visits to flowers in …

Drivers And Consequences Of Changes In Nectar Microbiome Composition Across Spatial And Biological Scales, Daniel A. Barker

Electronic Theses and Dissertations

Microbial communities inhabiting floral nectar, known as the nectar microbiome, are important mediators of plant-pollinator interactions. However, the ecological and environmental factors that shape the composition of these microbial communities remain poorly understood. While previous studies have examined the effects of a few microbial groups, typically independent of one another, on one plant or pollinator species, this dissertation takes a wide-scale approach to explore how abiotic conditions and biotic interaction’s structure microbial communities within floral nectar. Furthermore, I also quantify how variation in microbial richness and identity influences pollinator behavior and plant reproductive success.

This dissertation shows that microbial abundance …

Tradeoffs Between Elemental Homeostasis And Growth Govern Freshwater Phytoplankton Responses To Salinization, Samuel A.T. Dias, Clay Prater, Adriana Diaz-Delgado, Natalie A. Clay, Sally A. Entrekin, Michelle A. Evans-White

Entomology and Plant Pathology Faculty Publications and Presentations

Anthropogenic salinization resulting from road salt application can degrade aquatic environments by altering the structure and function of phytoplankton communities, ultimately reducing flows of resources through aquatic food webs. However, physiological mechanisms underlying taxon-specific responses to salinization are often poorly linked to higher-order ecosystem dynamics, limiting our ability to predict community responses to salinization. To this end, we tested hypotheses derived from Subsidy-Stress and Ecological Stoichiometry theory by growing two cosmopolitan genera, Dolichospermum (prokaryotic, cyanobacteria) and Scenedesmus (eukaryotic, green algae), across NaCl gradients and contrasting differences in their growth rates, degree of Na homeostasis, and cellular C : N : …

Impact Of S-Phase Kinase Protien 2 Blockades On Hematopoetic Stem Cell Metabolism, Nicole Elmaraghy

Electronic Theses, Projects, and Dissertations

Hematopoietic stem and progenitor cells (HSPCs) quiescence is vital for the success of bone marrow transplantation, as it preserves long term self- renewal and prevents premature exhaustion (Takubo et al., 2013; Wilson et al., 2008). However, bone marrow transplant (BMT) failure remains a clinical challenge, often due to lack of long-term engraftment and insufficient stress reliance. Both of these characteristics are tightly linked to disrupted stem cell quiescence and metabolic imbalance (Anso et al., 2017; Vannini et al., 2016). One key player is S-phase kinase protein (SKP2), an E3 ubiquitin ligase that targets cell cycle inhibitors, like p27, for proteosome …

Multicenter Span Trial Of Fasudil In Ischemic Stroke, Takahiko Imai, Andreia Lopes De Morais, Xuyan Jin, Tao Qin, Jessica Lamb, Karisma A Nagarkatti, Mu-Hsun Chen, Ligia S B Boisserand, Rakesh B Patel, Mariia Kumskova, Anjali Chauhan, Krishnan M Dhandapani, Mohammad B Khan, Pradip K Kamat, Yanrong Shi, Suyi Cao, Basavaraju G Sanganahalli, Joseph B Mandeville, Patrick D Lyden, David C Hess, Enrique C Leira, Anil K Chauhan, Jaroslaw Aronowski, Louise D Mccullough, Raymond C Koehler, Lauren H Sansing, Márcio A Diniz, Cenk Ayata

Children’s Nutrition Research Center Staff Publications

Background: The SPAN (Stroke Preclinical Assessment Network) is a confirmatory multicenter trial network to test cerebroprotective interventions in experimental acute stroke. In a first-of-its-kind trial, SPAN tested 6 interventions in a rodent model of transient focal ischemic stroke. Here, we report the efficacy of fasudil, an isoform-nonselective rho-associated kinase inhibitor, on primary and secondary outcomes in the SPAN trial.

Methods: Fasudil was administered at 10 mg/kg intraperitoneally every 12 hours for 6 doses starting 5 minutes before reperfusion in a 60-minute endovascular filament middle cerebral artery occlusion model. The active treatment arm (n=345) was compared with the pooled intraperitoneal and …

Family-Level Factors That Influence Physical Activity Among Hispanic Adolescents At Risk For Type 2 Diabetes, Sandra Mihail, Ayleen A Hernandez, Erica G Soltero

Children’s Nutrition Research Center Staff Publications

Objective: Family-based health promotion and disease prevention strategies are recommended as best practice; however, there is limited knowledge regarding the family-level factors that influence modifiable behavior risk factors like physical activity (PA) among Latinx adolescents. This study addressed this knowledge gap by using qualitative methods to identify perceptions of family-level factors that influence PA among Hispanic youth.

Methods: We conducted semistructured, open-ended interviews with 20 Latinx adolescents (14-16 years) with obesity (body mass index ≥95th percentile) to identify their perceptions of how family influences PA. Content analysis was used to identify emergent themes, which were then compared across demographic factors, …

Use Of Infrared Spectroscopy In Animal Studies: Aspects Of Health Science, Brian Manzanares

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Non-invasive imaging technologies, such as infrared thermography (IRT), near-infrared spectroscopy (NIRS), and fourier-transform infrared spectroscopy (FTIR) are revolutionizing the field of animal research by enabling real-time, stress-free assessment of physiological conditions. Such methods like these are align with the ethical standards of the 3Rs, replacement, reduction, and refinement-by reducing the need of invasive procedures while enhancing data quality and animal welfare. These methods can also provide critical insight into animal health, behavior, and stress responses without the need for invasive procedures or restraint, thereby minimizing harm and distress. This literature review will explore the practical applications of IRT, NIRS, and …

Improved Rumen Microbiome Interpretation Via Empirical Filtration Derived From Known Mock Microbiomes, Wesley A. Tom

School of Biological Sciences: Dissertations, Theses, and Student Research

The rumen microbiome plays a critical role in ruminant health and performance. As a consequence, many studies investigate microbial community composition using 16S rDNA based sequencing. As such, data quality and data filtering are critical to accurately identify microbial community composition. In this study we use mock communities to empirically select data filtering parameters to reduce artifact populations and compared the effect of filtering using different bioinformatic pipelines using rumen bacterial community data. The filtering parameters identified provides consistent estimates of rumen microbial diversity, regardless of bioinformatic pipeline utilized and provides a more accurate view of microbiome structure and composition. …

Validation Of Head Above And Head Submerged Hydrostatic Weighing Equations And Air Displacement Plethysmography, Axel M. Miller

All Graduate Reports and Creative Projects, Fall 2023 to Present

Traditional hydrostatic weighing (HW) involves full body submersion at residual volume (RV), which may be uncomfortable. Thus, the purpose of this study was to investigate a more comfortable way to perform this method. HW with the head above water (HW_HAW) was compared to the traditional method of HW with head below water (HW_HBW) and to another method that also measures density by displacement, air displacement plethysmography (ADP). Equivalence was tested by comparing the body fat percentage (BF%), body volume (Vb), and body density (Db) of 27 subjects (15 M, 12 F) between the three methods. Results …