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Agar Plate Culture Of Selected Trypanosomatidae, Amy Doran Keppel

School of Biological Sciences: Dissertations, Theses, and Student Research

Summary

The study of trypanosomatid biology would benefit greatly from the acquisition of a tool for culturing such as those used in microbiology. Agar plate culture has been described several times, but until the present its potential for use in this area has been underestimated. The previous work has demonstrated that this culture method may be used to culture a wide variety of species, that these species give rise to identifiable colony phenotypes, and that these are stable regardless of the life cycle stage used to initiate the culture. It was also shown that colonies could be initiated from the …

Progress Report For The Dairy Research Advisory Board, Various Authors

Other Documents

No abstract provided.

The Role Of Exercise And Nutrition In The Rehabilitation Of The Geriatric, Robert R. Ladd

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract not included]

Danos Causados À Soja Em Diferentes Níveis E Épocas De Infestação, Durante O Crescimento, Maria Helena M. Galileo, Elvis A. Heinrichs

Department of Entomology: Faculty Publications

Foram avaliados os danos causados à soja (Glycine max (L.) Merrill) por Edessa meditabunda (Fabricius, 1794), em diferentes níveis e épocas de infestação, usando-se gaiolas de campo, no ano agrícola 1973/74. Os níveis de dois, quatro, seis e dez insetos por gaiola, atuando do florescimento pleno até iniciar-se o enchimento da semente, bem como as infestações desde o florescimento pleno até o final da maturação fisiológica da semente, não reduziram o rendimento, o número de vagens e de sementes, o número de sementes por vagens e o peso médio da semente. Igualmente, a percentagem de sementes danificadas, em diferentes …

Detection Of Unique Tumor-Associated Proteins In The Urine Of Patients With Transitional Cell Carcinoma By High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis, Robert David Lehman

Biological Sciences Theses & Dissertations

A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer patients. Urine was chosen as a source of tumor-associated components because the location of the tum or should release already solublized tum or substances in to the urine.

Urinary proteins were concentrated and then analyzed by the two - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. …

93rd Annual Report Of The Nebraska Agricultural Experiment Station

Agricultural Research Division: News and Annual Reports

Contents:
Foreword
Administration and Staff
Nebraska Agricultural Experiment Station
Research Projects
Experiment Station Publications
Publications of the Staff
Report of Income and Expenditures

Percutaneous Transfemoral Renal Artery Occlusion In The Management Of Advanced Renal Carcinoma, Douglas E. Johnson

Henry Ford Hospital Medical Journal

This report describes renal artery occlusion as a therapeutic alternative to nephrectomy and chemotherapy in treating patients with metastatic renal carcinoma. Using a combination of Gelfoam and the Gianturco stainless steel coil, embolization was performed on 75 patients at the University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute with promising results. Its use is recommended 1) to facilitate surgical removal of large and difficult tumors, 2) to control local symptoms (pain, bleeding, etc), and 3) as part of a planned therapeutic program consisting of infarction, nephrectomy and progestins for patients with minimal to moderate metastatic disease.

Isolation And Identification Of Yeasts From The Barren River, Richard Vanenk

Masters Theses & Specialist Projects

The Barren River was sampled twice monthly for one year to obtain data on aquatic yeasts. Sites upstream and downstream of the Bowling Green sewage plant, as well as the sewage effluent, were sampled to ascertain if the effluent had any effect on the yeast population of the river. Sporadic yeast counts were obtained which averaged 40 CFU/ml for the effluent, while the river averaged 15 CFU/ml. A total of 318 yeasts were isolated and identified during the study, including 16 different genera with Cryptococcus being the most common. Cryptococcus laurentii occurred most frequently of the 67 different species identified. …

The Prairie Naturalist Volume 11 , Number 2. June 1979

The Prairie Naturalist

SPECIES WITH EXTRA FLORAL NECTARIES IN A TEMPERATE FLORA (NEBRASKA) ▪ Kathleen H. Keeler

PLANTS OF MOUNT GOLIATH RESEARCH NATURAL AREA ▪ Jeanette Hartman and Rod Mitchell

STATUS OF THE STURGEON CHUB (HYBOPSIS GELIDA) AND SICKLEFIN CHUB (HYBOPSIS MEEKI) IN NORTH DAKOTA ▪ Robert C. Reigh and Dean S. Elsen

OBSERVATIONS ON COYOTE BIOLOGY IN NORTH-CENTRAL MISSOURI ▪ Fred B. Samson and Bill D. Hill

DESTRUCTION TO NESTING BIRDS ON A MARSH BAY BY A SINGLE STORM ▪ Martin K. McNicholl

BOOK REVIEWS

Presentations on Predators ▪ Staff

Migratory Game Birds ▪ Ronald A. Ryder

Wolf …

Management Of Renal Cell Carcinoma: Evaluation Of Renal Masses By Modem Radiological Techniques, W. R. Eyler, M. A. Sandler, S. C. Cross, B. L. Madrazo

Henry Ford Hospital Medical Journal

The utilty of ultrasound and computed tomography is compared to angiography and that of nephrotomography in the study of renal masses. Ultrasound and computed tomography, noninvasive methods, are replacing nephrotomography and, in some cases, angiography

Isolation Of An Acid-Fast Organism From The Aqueous Humor In A Case Of Sarcoidosis, C. L. Barth, M. S. Judge, L. H. Mattman, P. C. Hessburg

Henry Ford Hospital Medical Journal

The anterior chamber fluid from the eye of this patient with sarcoidosis was found to contain microcolonies of a cellwall-deficient organism that was propagated and identified as acid-fast by the Intensified Acid-Fast stain. The colonies were inoculated into mice and retrieved from the dead or sacrificed animals. This report suggests that the acid-fast microbe in the aqueous humor of this case of uveitis-sarcoidosis may be the same organism as that found in the blood in sarcoidosis. Thus, it may be associated not only with the primary disease, but also with the complications of Boeck's sarcoidosis.

1978 Publications Of The Staff Of Henry Ford Hospital

Henry Ford Hospital Medical Journal

No abstract provided.

The Effect Of Escherichia Coli Endotoxin On The Cat Periapical Tissues, Thomas G. Dwyer

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical tissue reaction to three concentrations of endotoxin solutions and to a sterile saline control solution was examined in six adult cats. The maxillary and mandibular cuspids were isolated and the pulps extirpated with barbed broaches. The four solutions (1, 10, 100 μg/ml endotoxin, and saline) were then injected into the four prepared teeth for each cat and the access cavities sealed with IRM.

At two week intervals, two cats were sacrificed and the specimens removed, while the remaining animals were retreated as previously described. The block tissue sections were radiographed, fixed in 10% buffered formalin, and prepared for …

The Effects Of Temperature And Ph On The Differentiation Of Trypanosoma Lewisi (Kent, 1880) Laveran & Mesnil, 1901, In A Defined Medium, Carolann R. Rosario

Loma Linda University Electronic Theses, Dissertations & Projects

Trypanosoma lewisi is an arthropod-transmitted protozoan parasite found in the bloodstream of rats. In its life cycle it undergoes cellular differentiation. It is primarily found as the trypomastigote form in the rat and as the epimastigote form in the arthropod, the rat flea. Various studies have been done on hemoflagellates to determine the factors responsible for stimulating the differentiation from one form to the other. Included in these factors are pH, temperature, and protein fractions from fetal calf serum. Recent efforts have also been aimed at developing simple or defined media for physiological or biochemical studies on hemoflagellates. The purpose …

The Effects Of Two Polychlorinated Biphenyls (Aroclor 1016r And Aroclor 1242r) On The Growth And Chlorophyll Production Of Cultures Of Selenastrum Capricornutum, Jude William Grosser

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Jude William Grosser on May 11, 1979.

The Effect Of Rutin On Swine Serum Cholesterol And Lipoproteins, Jimmy R. Salyer

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Jimmy R. Salyer on May 8, 1979.

The Effect Of Quercetin-3-B-Rutinoside On Blood Coagulation And The Development Of Atherosclerotic Lesions In Swines, Milford C. Jarrells

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree on Master of Science in Biology by Milford C. Jarrells on May 8, 1979.

Cell Culturing Of Swine Aortic Smooth Muscle Cells, Rita E. Bustos

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Rita E, Bustos on May 2, 1979.

Androgen-Induced Immunosuppression, Debra Ann Weyant

Dissertations and Theses

It is well established that females are more immunocompetent than males as evidenced by higher humoral antibody titers, lowered susceptibility to infection, and more efficient graft rejection.

Furthermore, females also exhibit a much higher incidence of autoimmune disease. These observations have led investigators to believe that the male hormonal environment may play a key role in the regulation of immune response. For this reason, this study is concerned with the expression of autoimmunity and of immune function in the mouse.

This study included the New Zealand Black (NZB) mouse strain, as an animal model for human SLE, as well as …

The Effects Of Ph On 5-Oxogluconate Production By Gluconobacter Suboxydans, Neng-Chyang Leu

Masters Theses & Specialist Projects

The metabolism of glucose to 5-oxogluconate by Gluconobacter oxydans subsp. suboxydans (Gluconobacter suboxydans) at different pH values, glucose concentrations and phosphate buffer concentrations was studied. Bacterial growth, gluconic acid accumulation and 5-oxogluconate production were related to both pH and phosphate buffer concentration in the medium.

In media containing 2-3% glucose, 0.3% yeast extract and 0.3 M phosphate buffer, the optimal pH range for the bacterial growth was pH 6.4-7.0; the optimal pH range for gluconate production was pH 6.4-6.8; and the optimal pH range for 5-oxogluconate production was pH 6.6-7.0. Lowering the pH to 6.4 favored gluconate accumulation …

Spectral Signature Studies For Application In Deer Census Using Remote Sensing Techniques, Maran C. Pate

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was performed to determine the spectral signatures of deer and their natural background elements for censusing purposes. Consideration was given to atmospheric transmittance, acceptable flying weather, and terrain. Possible spectral bands between 0.3 and 14.0 μm were obtained (over a pathlength of 1500 feet at an altitude of 5000 feet) based upon atmospheric transmittance using the LOWTRAN 3B computer program. They are: 0.30 - 1.33, 1.49 - 1.79, 2.00 - 2.50, 3.00 - 3.16, 3.38 - 4.10, 4.59 - 5.05, and 8.00 - 13.33 μm, for transmittance greater than 75%. Weather conditions are favorable for flying and taking …

Estimation Of The Pantothenic Acid Content Of Foods Using A Microbiological Assay And A Radioimmunoassay, Joan Howe Walsh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Seventy-five processed and prepared foods commonly consumed in the United States were analyzed for pantothenic acid. The foods were analyzed using the traditional microbiological assay for the vitamin and using a new radioimmunoassay (RIA).

The preparation of food sample extracts for both assays was modified. After the food samples underwent enzyme hydrolysis, the food-enzyme mixture was dialyzed to obtain a clear extract for use in the assays. Previously, the food-enzyme mixture was poured through filter paper for clarification.

A very high correlation (r²= .937) between the results from the RIA and the microbiological assay was found. There was …

Enlightening Consumer Nutrition Decisions: Comparison Of A Graphical Nutrient Density Labeling Format With The Current Food Labeling System, Kristy Gregerson Mohr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Providing consumers with usable nutrition information requires an effective labeling format. The objective of this study, which was conducted in a supermarket setting, was to determine whether consumers could, without previous instruction, make equally effective nutrition decisions using a graphic format based on nutrient density as when using the current labeling format. For comparison with other studies, a demographic, nutrition knowledge and nutrition labeling data base was collected. The questionnaire completed by each participating consumer included items regarding demographic data and shopping preferences, and questions evaluating nutrition knowledge for comparison as a data base with other studies. It also appraised …

Growth Of Phytophthora Infestans Race 1.2.4 And Synthesis Of Steroid Glycoalkaloids By The Fungus In Synthetic Media, Melanie R. Maas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A chemically defined synthetic medium was developed in which Phytophthora infestans (Mont.) de Bary race 1.2.4 grew extensively. This medium consisted of: 90.0 grams maltose, 20.0 grams DL-alanine, 20.0 grams L-proline, 1.0 gram KNO₃, 0.5 gram KH₂PO₄, 0.25 gram MgSO_4. 7H₂O, 1.0 milligram thiamin per liter of distilled water. The average level of growth in this medium was 0.5864 gram dry weight mycelium per 50 milliliters medium.

The glycoalkaloids, solanidine and solanine, were synthesized by Phytophthora infestans in the above medium with 62.5 percent of the samples positive and an overall …

Incorporating The Index Of Nutritional Quality In The Nutrition Education Of Wic Recipients, Mary J. Roth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop and evaluate nutrition educational lessons and instructional materials based on the Index of Nutritional Quality (INQ). INQ is a graphic, nutrient density concept. The lessons and instructional materials were used in the nutrition education of pregnant women in the WIC Program. Six nutrition lessons were written utilizing the INQ concept. Lesson one emphasized the importance of the WIC supplemental foods and the recommended number of servings from the basic four food groups during pregnancy. Lessons two through six concerned the importance of the following nutrients during pregnancy: calcium, protein, iron, vitamin A …

Effects Of Acid Fermentation, Dry-Heat And Wet-Heat Processing On The Bioavailability Of Calcium, Iron And Fluoride In Mechanically-Deboned Beef, Tzy-Bin Nancy Tso

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mechanically-deboned beef shank (MDS) and hand-deboned beef shank (HDS) were prepared into thuringer (acid fermented), canned meat (retorted) and bologna (dry-heated). Raw meat and these products were then lyophilized and mixed into balanced diets and fed to weanling male rats for three weeks to evaluate the bioavailability of calcium, iron and fluoride. Bioavailability of calcium from MDS and its processed products was determined by feeding growing male rats with 5.01 to 5.53 g Ca/kg from MDS. HDS diets with 0.18 to 7.13 g Ca/kg added from calcium carbonate were also fed to rats for a relative comparison. The bioavailability of …

Sodium And Potassium Nutrition Studies Of Halogeton Glomeratus (M. Bieb) C.A. Mey, Ali A. Alhelal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Halogeton glomeratus (M. Bieb) C.A. Mey when grown in nutrient solution, caused the solution pH to decrease very rapidly as plants increased in size, especially during the fifth week of growth. This might be attributed to hydrogen ion exchange for cations. Also, the cation uptake, especially K and Na, may exceed that of the major anions. The control of pH is effective in increasing shoot weights markedly.

In general there was no difference between plants grown at the same NaCl concentration at low and high relative humidity. An increase in plant size, shoot weights, leaf length and diameter was detected …

A Comparison Of Vocational Agricultural Facilities In Utah High Schools And Those In Selected States Of The Western Region As Perceived By Vocational Agriculture Teachers, Ray J. Tubbs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was limited to vocational agricultural teachers in Utah and ten agricultural teachers randomly selected from each of the following states: Arizona, Colorado, Idaho, Montana, Nevada, New Mexico and Wyoming.

Questionnaires were completed and returned by a total of 54 Utah vocational agricultural teachers and 50 teachers from the other states. The questionnaires were designed to collect data concerning class size, requirements of the physical facilities and the budget allowed for teaching agricultural mechanics.

The study revealed a larger number of non-vocational agriculture students are enrolled in the agricultural programs in Utah than in the other states. The data …

Bacteriological Quality Of Runoff Water From Pastureland, John W. Doran, D.M. Linn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Runoff from a cow-calf pasture in eastern Nebraska was monitored for total coliforms (TC), fecal coliforms (FC), and fecal streptococci (FS) during 1976, 1977, and 1978. Bacteriological counts in runoff from both grazed and ungrazed areas generally exceeded recommended water quality standards. The FC group was the best indicator group of the impact of grazing. Rainfall runoff from the grazed area contained 5 to 10 times more FC than runoff from the fenced, ungrazed area. There was little difference in TC counts between the two areas, but FS counts were higher in runoff from the ungrazed area and reflected the …

Cell Mediated Immunity By Cytotoxicity Assay And The Effect Of Corynebacterium Parvum And Radiation On Mice Bearing Herpes-Induced Tumors, Robert B. Stagg

Loma Linda University Electronic Theses, Dissertations & Projects

Cell mediated immune (CMI) cytotoxic reactivity of Balb/c mice against H238 cells, a Herpes simplex virus (HSV) Type 2 - induced sarcoma, was measured by the ¹²⁵IUdR release assay. The Balb/c mouse response to the growing HSV-induced tumor, treated with radiation and , was measured by survival rate, tumor growth and immune cytotoxicity of spleen and peritoneal exudate cells (PEC) as determined by ¹²⁵IUdR release assay. Subcutaneous (s.c.) inoculation of 1 x 10⁶ H238 cells (high dose) produced progressive tumor growth while s.c. inoculation of 1 x 10⁴ H238 cells (low dose) produced no tumors. A …