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State Of Cytidine 3', 5' Cyclic Monophosphate (Cyclic Cmp) Research, Joseph E. Stone, Bruce E. Murphy

Journal of the Arkansas Academy of Science

No abstract provided.

A Water Quality Survey Of The Big Blue River, Nebraska, Terry R. Maret, Curtis C. Christiansen

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Physiochemical and bacteriological conditions along with the macroinvertebrate community structure were studied to evaluate water quality of the Big Blue River, in southeastern Nebraska. Samples were taken between November 7, 1978, and December 19, 1979, at six stations from the headwaters to lower reaches of the river. Some sample sites were below specific municipal and industrial effluents to evaluate better their impact on water quality. Parameters measured included pH, temperature, dissolved oxygen, suspended solids, conductivity, biochemical oxygen demand, nitrate, ammonia, and fecal coliforms. Dissolved oxygen and nitrate were significantly different (p<0.05) between stations, with the headwaters station exhibiting the greatest difference in water quality. Fecal coliforms were substantially higher at stations below Crete. The macroinvertebrate fauna comprised 42 and 66 taxa collected with Ekman grabs and multiplate artificial substrates, respectively. Average diversity values, using the Shannon-Weaver index, indicated all stations would be categorized as moderately polluted. Taxa collected at the stations showed a great deal of homogeneity. An exception was the headwaters site where pollution-associated taxa were found in abundance. Seasonal changes were an apparent factor affecting diversity of the macro invertebrates. The results collected provide useful baseline information for future evaluation of water quality trends of the Big Blue River.

Intercellular Communication In Plants: Evidence For A Rapidly Generated, Bidirectionally Transmitted Wound Signal, Eric Davies, Anne Schuster

School of Biological Sciences: Faculty Publications

Wounding (whether by excision, abrasion, or puncture) elicited rapid, massive, and enduring formation of polysomes in aged pea stems and other mature tissues. The response depended on temperature and severity of wounding but not on water uptake, and it occurred in tissues adjacent to, distant from, above, and below the site of injury. The kinetics of polysome formation were similar in tissues adjacent to or up to 150 mm distant from the point of injury. The wound-induced increases in proteinsynthesizing capacity of the polysomes both in vivo and in vitro were much greater than the increases in rRNA and poly(A) …

Description And Taxonomic Importance Of Penial Morphology In The Cryptodiran Turtles Of Malaysia, Lynn D. Wike

Masters Theses

This paper investigates the use of penial structure to study the relationships among the turtles of peninsular Malaysia. The penial morphology of Malaysian turtles, mainly batagurines, has not been previously described. Herein penes of 12 Malaysian species are described and compared to three Nearctic species. Each was coded for 22 penial characters which were used for numerical analysis by the NT-SYS numerical taxonomy system of multivariate statistical proceedures. The analysis failed to distinguish groups below the family level when the 15 species were tested. The test also failed to distinguish the complexes within the Batagurinae when they were analyzed separately. …

The Role Of Suppressor T Lymphocytes In Renal Allograft Rejection, Fred Barry Pearlman

Master's Theses

No abstract provided.

Non-Suppressible Insulin-Like Activity, Glucose Hypercatabolism, And Hypoglycemia In Sepsis, Rico E. Viray

Master's Theses

No abstract provided.

Selection Of Somatic Cell Hybrids Between Baby Hamster Kidney Cells And Human Embryonic Kidney Cells To Study Bk Virus Early Gene Expression, Sara Lynn Ehlke

Master's Theses

No abstract provided.

Changes In Myosin And Myosin Light Chain Kinase During Myogenesis, Stylianos P. Scordilis, J. Maurice Miles, Robert S. Adelstein, B. William Uhlendorf, Sigfrido Scarpa, Giulio L. Cantoni

Biological Sciences: Faculty Publications

Myosins and myosin light chain kinases have been isolated from a cloned line of myoblasts (L5/A10) as this cell line undergoes differentiation toward adult muscle. At least three myosin isozymes were obtained during this developmental process. Initially a nonmuscle type of myosin was found in the myoblasts. The molecular weights of the myoblast light chains were 20 000 and 15 000. Myosin isolated from early myotubes had light chains with molecular weights of 20 000 and 19 500. Myosin isolated from myotubes which contained sarcomeres had light chains with molecular weights of 23 000, 18 500, and 16000. This last …

Genetic Studies Of Questionnaire Data From A Residential School For The Deaf, Frederick Robert Bieber

Theses and Dissertations

A self-administered thirteen page Hearing Loss Questionnaire (HLQ) was designed in order to systematically collect medical and family history data on deaf children and their families. Data were collected from over 400 families with one or more children enrolled in September 1979 at the Maryland School for the Deaf (MSD). Almost 70% of the parents provided pedigree and family history information by completing the detailed HLQ. Computer analyses of the collected data allowed a thorough examination of almost 200 medical and family history variables, providing useful reference data on the MSD probands. Parental responses to a four-step rating scale of …

Solubilization And Partial Characterization Of An Activity Regulating Complement Activation From Membranes Of Raji And Tonsil Cells, Patricia L. Carlson

Theses and Dissertations

Complement is one of the major innate nonspecific mechanisms for host defense in serum. More than a dozen proteins have been isolated from serum which have a function in either the activation or regulation of complement action. Complement is similar to the coagulation sequence in that it comprises a number of precursor proenzymes in plasma that are sequentially cleaved to generate biologically and enzymatically active proteins.

Ecological And Genetic Separation Of Three Sympatric Species Of Aedes (Diptera: Culicidae) From The Kenya Coast, L. P. Lounibos, L. E. Munstermann

All Peer-Reviewed Publications

Aedes ledgeri Huang, A. soleatus Edw. and A. calceatus Edw. of the group of A. aegypti (L.) of the subgenus Stegomyia collected on the coast of Kenya are distinguished on the strength of ecological and genetic (isozyme) data. The preferred oviposition sites of A. ledgeri and A. soleatus were tree holes above ground level, whereas A. calceatus favoured abandoned gastropod shells. A. ledgeri was most abundant in ecotonal habitats and A. soleatus in undisturbed forest At least two diagnostic enzyme loci separated each of the three species pairs. © 1981, Cambridge University Press. All rights reserved.

Electron Spin Resonance Studies Of Membrane Fluidity Of Normal And Malignant Human Neural Tissues, Robert P. Wersto

Dissertations

No abstract provided.

Ratoon Stunting Disease Of Sugarcane: Isolation Of The Causal Bacterium, Michael J. Davis, A. Graves Gillaspie, Russell W. Harris, Roger H. Lawson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A small coryneform bacterium was consistently isolated from sugarcane with ratoon stunting disease and shown to be the causal agent. A similar bacterium was isolated from Bermuda grass. Both strains multiplied in sugarcane and Bermuda grass, but the Bermuda grass strain did not incite the symptoms of ratoon stunting disease in sugarcane. Shoot growth in Bermuda grass was retarded by both strains.

An Analysis Of Cadmium And Lead Uptake In Hydroponically Grown Corn Seedlings, Julie Jones Medlin

Dissertations

The purpose of this study was to analyze selected growth parameters of hydroponically grown corn seedlings (Zea mays) in relation to the uptake of the heavy metals, lead and cadmium. The corn seedlings were treated with various concentrations of lead and cadmium at different levels of pH, and phosphate and ferric ion concentrations. Root and shoot lengths were measured before and after heavy metal treatment to determine the relative growth in the different treatment solutions. Wet and dry weights were also obtained. The plant material was digested with nitric and perchloric acids in preparation for analysis for heavy metal content …

Growth Of Enterobacter Cloacae In The Presence Of 25% Sodium Dodecyl Sulfate, Vance C. Kramer, Diane M. Calabrese, Kenneth W. Nickerson

Papers in Microbiology

The growth of Enterobacter cloacae in 25% sodium dodecyl sulfate is described. The bacteria appeared to tolerate sodium dodecyl sulfate rather than metabolize it. The process was energy dependent, and cell lysis occurred during stationary phase. Extreme detergent resistance may be characteristic of the genus Enterobacter.

Diversity And Dynamics Of Indigenous Rhizobium Japonicum Populations, K. Dale Noel, Winston J. Brill

Biological Sciences Faculty Research and Publications

A simple method, based upon the separation of cellular proteins by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, has been devised for distinguishing between isolates of Rhizobium japonicum. Eleven laboratory strains, previously classified into five serogroups, were analyzed by gel electrophoresis. Groups determined subjectively according to protein patterns matched the serogroups, with one exception. Most strains within serogroups could be distinguished from one another. For studying the ecology of Rhizobium, an important advantage of this technique compared with serology or phage typing is that it discriminates among previously unencountered indigenous bacterial isolates as well as among known laboratory strains. …

The Psychological Relations Hip Between Dairy Cows And Dairy Cowmen And Its Implications For Animal Welfare, Martin F. Seabrook

International Journal for the Study of Animal Problems

No abstract provided.

The Beef Delivery System: Optimal Plant Sizes, Locations And Product Flows, J. C. Hafer, J. G. Kendrick

Nebraska Agricultural Experiment Station: Historical Research Bulletins

The United States beef delivery system is composed of three stages: procurement, processing, and distribution. Minimization of costs associated with these stages can be accomplished best by identification of optimal plant sizes and locations. Research was directed toward an analytical approach that could be used to minimize total costs of the U.S. beef delivery system. This report will discuss the grid system used to divide the · United States into study units, describe features of the model used, discuss research results, and offer conclusions.

Functions Of Deadenylation Factors In Mrna Decay And Mrna Processing Body Formation, Dinghai Zheng

Dissertations and Theses (Open Access)

Most newly synthesized messenger RNAs possess a 5’ cap and a 3’ poly(A) tail. The process of poly(A) tail shortening, also termed deadenylation, is important for post-transcriptional gene regulation, because deadenylation not only leads to mRNA translational inhibition but also is the first step of major mRNA degradation. Translationally inhibited mRNAs can be stored and/or degraded in dynamic cytoplasmic foci termed mRNA processing bodies, or P bodies, which are conserved in eukaryotes.

To shed new light on the mechanisms of P body formation and P body functions, I focused on the link between deadenylation factors and P bodies. I found …

Cellulase Activities Of Soil Fungi, Jacobo Ortega

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The cellulase activities of 8 isolates of fungi obtained from agricultural soils of Hidalgo County, TX were investigated by measuring the changes in the viscosity of a buffered solution of carboxymethylcellulose (CMC), produced by the fluids obtained from liquid cultures of these isolates. The change in the viscosity of the reaction mixture incubated at constant temperature (30 C) was measured with Cannon-Fenske routine viscometers. A buffered cellulase solution was used as a control.

Due to the variability that existed among the isolates investigated, it was possible to select active producers of cellulase with the method followed in this investigation. The …

Roadside Zoos Are Not Zoos

Close Up Reports

The HSUS was one of the first national animal welfare organizations to take a long, hard look at all zoos, including roadside menageries. During the last nine years, we have worked intensively, conducting zoo inspections, public education and awareness programs, and developing a working relationship with the professional zoological community as well as U.S. Government agencies charged with the welfare of animals.

Roadside Zoos Are Not Zoos

Close Up Reports

HSUS launches campaign against more than 1,000 menageries of misery

Dimensions And Characteristics Of The Retail Market For The City Of Brookings, South Dakota, A. Vollmers, Rodney L. Linafelter, Dean Tucker

Economics Research Reports (1969 - 2017)

This study was designed to examine the 1978 retailing environment in the City of Brookings, South Dakota. A survey of over 1,000 consuming households within the Brookings area was conducted in 1978 in establishing a data base from which this analysis proceeded. The basic purpose of this consumer survey study was to help establish the extent and trends in the Brookings retail and service market. It is the purpose of this report to provide the merchants of Brookings with infonnation which will assist them in improving their operations and to help the~ to be better able to serve and meet …

Pisolithus Tinctorius Mycobiont Inoculations As A Factor In Performance Of Containerized And Bare-Root Shortleaf Pine Seedlings On Lignite Minesoils In Panola County, Texas, Hoy Lee Bryson

Electronic Theses and Dissertations

The effects of artificial soil infestation with basidiospores and vegetative mycelia of the fungal symbiont Pisolithus tinctorius on ectomycorrhizae development of short leaf pine (Pinus echinata) seedlings grown in styroblock containers were tested in the greenhouse. These preliminary tests showed that both spores and mycelia will produce satisfactory ectomycorrhizae development. Various methods of inoculation had little effect on growth and development of containerized seedlings or on the accumulation of N, P, K, Ca, Mg, Na, Mn, Zn, or Cu in foliage and lateral roots. The styroblock containerization system used in conjunction with sandy loam soil/vermiculite (2:1 v/v) potting-mix produced excellent …

Comparison Of Three Mass Media For Communicating Forestry Information For Young People, Ross Lee Tomlin

Electronic Theses and Dissertations

A comparison of three mass media ( TV, radio and newspaper) was made to find which would be more effective at communicating forestry information to young people. The field study was conducted at Nacogdoches, Lufkin, and Kilgore High Schools in East Texas. Questionnaires given to students before and after exposure to six media spots tested forestry knowledge and attitudes. One spot in each medium presented a hardsell threat approach, the other a softsell-humorous approach. A final questionnaire measured student preferences of the media. Analysis of data indicated newspaper and TV to be the most effective media, with radio less effective. …

Multiple Forms Of Nicotinamide Adenine Dinucleotide Glycohydrolase From Bull Semen, Stanley D. Yeatts Ii

Chemistry & Biochemistry Theses & Dissertations

Nicotinamide adenine dinucleotide glycohydrolase (NADase) of bull semen is an extracellular, soluble enzyme capable of catalyzing the hydrolysis of the nicotinamide N-ribosidic linkage of NAD⁺. In search for a rapid, simple procedure for the preparation of this enzyme, it was found that Matrex Gel Red A (Amicon) column packing served very well as an affinity gel for NADase. The enzyme was applied to the Matrex Red A column (21 cm x 1.5 cm) in 10 mM sodium phosphate buffer, pH 6.0. Surprisingly, two fractions of NADase activity were obtained when the column was eluted with a sodium chloride …

The Analysis Of S-Adenosylmethionine And S-Adenosylhomocysteine In Normal And Neoplastic Cells, Kathryn Elizabeth Godburn

Chemistry & Biochemistry Theses & Dissertations

Altered patterns of methylation have been shown in both ribosomal and transfer RNA isolated from cancer cells. In contrast to the elevated activity of the methyltransferases responsible for this modification, hypomethylation appears as a general characteristic. The endogenous levels of S-adenosylmethionine and S-adenosylhomocysteine are important in relationship to their role as substrate and product of the transmethylation reaction. The levels of S-adenosylmethionine and S-adenosylhomocysteine were determined using phosphocellulose cation exchange or high pressure liquid chromatography. High voltage paper electrophoresis was used in a modified procedure to directly resolve (³⁵s) labeled S-adenosylmethionine and S-adenosylhomocysteine in small quantities of cell …

The Effects Of Elevated Calcium Chloride And Magnesium Chloride Levels On Larval Growth And Survival Of Macrobrachium Rosenbergii (De Man) (Decapoda Palaemonidae) Reared In The Laboratory, James Thomas Michaels Ii

OES Theses and Dissertations

The effects of elevated CaCl₂ , MgCl₂ , combination CaCl₂ and MgCl₂ concentrations and their associated ionic strengths on larval growth and survival of the freshwater prawn, Macrobrachium rosenbergii were investigated in the laboratory. Larvae were reared in diluted seawater of 15 parts per thousand salinity with elevated calcium concentrations of 330 parts per million and elevated magnesium concentrations of 1275 parts per million. Control larvae were also reared with elevated NaCl concentrations to duplicate the ionic strength increases of the combination CaCl₂ - MgCl₂ test solution which had the highest ionic strength. A …

Germination And Haustorial Development In Orobanche Minor Smith (Orobanchaceae), David L. Spelce

Biological Sciences Theses & Dissertations

Orobanche minor Smith is an introduced root parasite that is known to attack some commercial crops. Germination occurs only after a preconditioning period involving moisture which must be followed by a chemical stimulant produced by the host root. Strigol and strigol analogs (GR-compounds} have been reported to germinate some species of Orobanche. Statistical tests indicate a significant difference between the effectiveness of strigol, GR-7 and GR-45 (P = 0.001).

The embryo is an undifferentiated mass of cells. During germination, the radicular pole of the embryo differentiates into the radicle, while the plumular pole remains undifferentiated. The radicle forms the primary …

Orthognathic Surgical Expansion Procedure Of The Mandible Of Female Baboon Papio Sp?, Willis L. Schlenker

Loma Linda University Electronic Theses, Dissertations & Projects

Expansion of the maxillae is a well recognized procedure in orthodontics. Little is known about Mandibular Expansion. This project is a pure research of the possibility of expanding the mandible laterally at the symphysis on a baboon.

The symphysis was cut A-P and expanded laterally 11 mm, then held and stabilized with a titanium mesh work screwed to the inferior border of the mandible. The defect area was filled with a bone graft of marrow and cancellous bone fragments taken from the greater trochanter and the crest of the ilium. New bone did fill in the defect and the expansion …