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Cole1 Copy Number Mutants., Londa Schmidt, Joseph Inselburg

Dartmouth Scholarship

A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by …

Progress Report For The Dairy Research Advisory Board, Various Authors

Other Documents

No abstract provided.

Farm, Ranch And Home Quarterly Institute Of Agriculture And Natural Resources, University Of Nebraska- Lincoln

Farm, Ranch and Home Quarterly

In this issue:

An Investment Returned ....................... 2

The Northeast Station- 25 years of Research, Extension and Service......... 3

Opportunities and Obstacles- Women Choose Agricultural Careers.............. 11

The Animal Welfare Movement- Where did it come from and where is it going?.............. 14

Center Pivot Irrigation- Runoff Analyzed on Sloping, Clayey Soil .................. 16

Nitrogen Management- Soils Change in Nutrient Content......................... 18

New Directions in Research ................................ 20

Louisiana Agriculture Magazine, Summer 1982, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Isomers Of 5.8s Ribosomal Rna, David Smith

Chemistry & Biochemistry Theses & Dissertations

When whole cell RNA was separated by high resolution PAG (i.e., polyacrylamide gel) electrophoresis, 5.8S rRNA migrated as three higher mobility intense bands, and three faint bands of lower mobility. The three intense bands represent conformational isomers which migrated as a single band under denaturing conditions on PAG. The three faint bands represent conformational isomers of a minor form of 5.8S rRNA which is elongated at the 5' terminus of the molecule. This minor form migrates as a single band of lower mobility than the major form when electrophoresed on a denaturing PAG. The minor form was determined to have …

Determining The Uptake And Possible Assimilation Of The Plant Component Of Spartina Detritus By Palaemonetes Pugio Using A Rapid Radiolabeling Method, Michael Peter Crosby

Biological Sciences Theses & Dissertations

Assimilation of the plant components of Spartina alterniflora detritus by the grass shrimp, Palaemonetes pugio, was investigated in a laboratory feeding study. A new radiolabeling procedure was utilized to label the sterilized detritus with ¹⁴C. Organic carbon values were calculated for both S. alterniflora and P. pugio. The grass shrimp were found to assimilate significantly the detritus, with an assimilation efficiency of approximately 14%, and an approximate ingestion rate of 2.508 x l0^-4mg C detritus/mg C shrimp hour^-l occurred. It is hypothesized that coprophagous activity may be important to the completion of digestion of …

Isoenzyme Update: Creatine Kinase And Lactate Dehydrogenase, Craig C. Foreback

Henry Ford Hospital Medical Journal

Creatine kinase (CK) and lactate dehydrogenase (LD) are isoenzymes that have been measured in clinical laboratories for over 20 years; their separation has proved valuable in the diagnosis of myocardial infarction and other cardiac-related diseases. Although, historically, electrophoresis was the preferred method to measure both isoenzymes, there has been controversy over the best method for separating the CK isoenzyme. lon exchange chromatography has been used successfully to isolate CK isoenzymes, and the procedure was adapted to determine CK-MB activity in serum. New methods to quantify CK-MB have also been tested, including an automated column technique, the immunoinhibidon/ immunoprecipitadon technique, and …

The Cytoskeletal System Of Nucleated Erythrocytes. I. Composition And Function Of Major Elements, William D. Cohen, Diana Bartelt, Richard Jaeger, George M. Langford, Iris Nemhauser

Biology - All Scholarship

We have studied the dogfish erythrocyte cytoskeletal system, which consists of a marginal band of microtubules (MB) and trans-marginal band material (TBM). The TBM appeared in whole mounts as a rough irregular network and in thin sections as a surface-delimiting layer completely enclosing nucleus and MB. In cells incubated at 0 degrees C for 30 min or more, the MB disappeared but the TBM remained. MB reassembly occurred with rewarming, and was inhibited by colchicine. Flattened elliptical erythrocyte morphology was retained even when MBs were absent. Total solubilization of MB and TBM at low pH, or dissolution of whole anucleate …

Comparison Of Grouping Methods For Beta-Hemolytic Streptococci, Renee Smith

Masters Theses & Specialist Projects

A total of 198 strains of beta-hemolytic streptococci were Lancefield grouped by the conventional nonserological method and the Phadebact Co-agglutination method. These two methods were then compared to the Lancefield precipitin test. The Phadebact Co-agglutination method and the non - serological method '.ere evaluated as to their sensitivity, accuracy, and suitability as methods for serogrouping betahemolytic streptococci in clinical laboratories. Due to the difference in their grouping ability only Lancefield Groups A and B could be directly compared. There was a 100 percent agreement between both of these methods for the Lancefield grouns A and B, and there were three …

Improved Fermentation Process For Producing Methane From Cheese Whey, Abdulraman Y. Awad

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two methods of single-stage fermentation were used to produce methane from whey. The first method involved batch fermentation in which the pH was automatically controlled at 7.0. In addition to pH control, the second method was characterized by introducing the substrate continuously into the fermenter along with dilution water to keep the organic acids at a non-toxic level.

The first method did not improve the production of methane compared to the batch fermentation in which a pH control system was not used (41). However, the second method showed that the concentration of organic acids has a major effect on limiting …

The Relative Roles Of Initial And Residual Sodium Nitrite On Germination Of Clostridium Botulinum Spores In Meat, Orchid Mettanant

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Four levels of NaNO₂ (0 ppm, 50 ppm, 100 ppm, and 156 ppm) were tested for antibotulinal activity in ground pork inoculated with spores of Clostridium botulinum either at the time of formulation or after cooking. Samples formulated with less than 156 ppm sodium nitrite received additional nitrite to adjust the residual nitrite equal to that found after cooking in samples formulated with 156 ppm nitrite. All samples were subjected to abusive storage at 27 C. Inoculating the spores at the time of formulation resulted in a faster rate of swelling. Heating C. botulinum spores for 30 min. at …

Evaluation Of Heme And Free Iron Binding Agents As Substitutes For Sodium Nitrite In Cured Meat, Farzaneh Vahabzadeh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitric oxide (NO) and carbon monoxide (CO) gases, alone or with oxalate, phytate, or ethylenediaminetetraacetate (EDTA) were tested for antibotulinal activity as substitutes for sodium nitrite in ground pork inoculated with spores of Clostridium botulinum, then abused by storage at 277°C. Nitric oxide with 250 ppm oxalate or phytate was most inhibitory, while NO alone was as effective as 156 ppm sodium nitrite for inhibition of gas and botulinal toxin production in the meat system. All swollen samples contained very low levels of residual nitrite, but nitroso heme and soluble iron content did not change compared with unswollen samples …

A Nutrition Education Componenet For Migrant Children Based On The K-6 Nutrient-Density Curriculum, Sandra Beadleson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A nutrition education component was developed, implemented, and evaluated for integration into the Title I migrant education program. The component was based on an evaluated nutrition education curriculum implemented by Brown in the elementary school. The nutrition education program was developed on the concept of nutrient density which measures the ratio of a food's nutrient contribution to its caloric contribution on a constant caloric basis. The nutrition component was modified to include behavioral objectives and concepts that partially met the needs of a migrant population in Utah.

The program was evaluated in the Title I programs in Corrine and Smithfield, …

Toxicologic Effects Of Extracts Of Cestrum Parqui L'Héritier In Mice, Tomás A. López

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cestrum parqui L'Héritier is a Solanaceous weed of wide distribution throughout the world. It causes poisoning of animals, particularly cattle and sheep, and constitutes a serious problem for animal production in some countries.

The present study was undertaken to develop a biological test for the detection of toxicity of extracts obtained from the plant. This test will be utilized to assess the toxicity of extracts obtained from the plant in future studies.

The plant is known to induce poisoning in laboratory animals, but there are no precise references on doses to be utilized and of appropriate routes of dosing. Oral …

The Characterization Of Carbamate Resistance In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), In Comparison With The House Fly, Musca Domestica (L), Randy L. Rose

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Resistant and susceptible populations of the Colorado potato beetle, Leptinotarsa decemlineata (Say), and the house fly, Musca domestica (L), were compared with respect to carbofuran and carbaryl toxicity in the presence and absence of the synergist, piperonyl butoxide. Resistance levels of the New Jersey population when compared with the susceptible Logan population by topical application of carbaryl and carbofuran were > 833 and 820, respectively. A resistance level of 583 was determined from carbofuran bioassays of Rutgers and NAIDM house flies. Similar levels of resistance development between these species suggests the possibility that similar resistance mechanisms may be involved.

Utilization of …

A Revision Of The North American Species In The Genus Euodynerus (Hymenoptera: Eumenidae), Ali Shallan Moalif

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twenty-eight species of Euodynerus Dalla Torre are recognized from the Nearctic Region, of which one is Holarctic. Literature on the biology of fourteen species is reviewed and new biological information discussed for four species. The phylogenetic relationships of the genus Euodynerus to other genera and the classification and relationships of the species of Euodynerus are discussed. Seven subgenera are recognized of which four are new: Euodynerus, Pareuodynerus Bluethgen, Leucodynerus Bohart, Annodynerus new subgenus, Gonodynerus n. subgenus, Holodynerus n. subgenus and Plecodynerus n. subgenus. Their comparative morphology, phylogeny, and distrubution are discussed. Larval instars of E. hidalgo (Saussure), E. scutellaris …

Evaluation Of Allergic Hypersensitivity To 2,4-D, Malathion, And Captafol In Mice, Janette R. Cushman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

2,4-D-specific IgE antibodies were detected in serum of BALB/c mice using the rat passive cutaneous anaphylaxis (PCA) test following the second intraperitoneal immunization with 1, 10, or 100 ug of 2,4-D0keyhole limpet hemocyanin (KLH) conjugate administered with aluminum hydroxide adjuvant. The groups that received 1 ug of 2,4-D conjugate had the highest antibody titers (geometric mean of 60). A paper radioallergosorbent test (PRAST) was developed for determination of 2,4-D-specific IgE. The PRAST was equally as sensitive as and showed a positive correlation with the PCA assay.

The anhydride of the diacid metabolite of malathion (MMA) coupled to KLH elicited MMA-specific …

The Isolation And Characterization Of A Novel Iron Protein From Desulfovibrio Gigas, Alan Kay Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The isolation, purification, and partial characterization of a novel iron-containing protein from the sulfate-reducing anaerobic bacterium, Desulfovibrio gigas, is described. The highly insoluble protein was isolated from the cell debris following osmotic shock of the bacteria. The insoluble fraction consistently contained about 90% of the cell-associated iron. This protein was treated with acid, chelating agents, detergents and proteases in order to study their effect on the iron solubility. The results of elemental analysis of a crude protein preparation were 5.3% iron, 2.9% sulfur and 11 .9% nitrogen. An independent colorimetric iron analysis showed 6.4% iron. The iron could be …

Tonsil Cell Products Which Modify In Vitro Proliferation Of Blood Lymphocytes, Thomas W. Hodge

Electronic Theses and Dissertations

Human palatine tonsil lymphocytes, when compared to peripheral blood lymphocytes (PBL), were in an activated state even though there was no in vitro stimulation. When these tonsil lymphocytes were cultured in the absence of serum and polyclonal mitogens or antigens, the supernatant fluid often inhibited the proliferative response of target PBL to con A. The extent of this suppression ranged from 22% to 84%, and target cell viability was 90% or greater. There was no evidence for the presence of immunoglobulins or (alpha)2-macroglobulin in whole supernatant fluids. The suppressor was partially denatured at 80(DEGREES)C and was rendered completely inactive upon …

Use And Evaluation Of The Nutrient Density Concept For Assessing The Impact Of Socioeconomic Factors On Nutritional Quality Of Diets, Carol Thompson Windham

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Data from 7285 individual participants in the USDA Spring Nationwide Food Consumption Survey were analyzed using the nutrient density concept and multiple regression procedures to evaluate the impact of socioeconomic status on the nutritional quality of foods consumed. For each socioeconomic group the average daily amount of nutrients consumed per 1000 kcal of food consumed were computed and compared with the Recommended Dietary Allowances which had been converted to single-value nutrient allowances per 1000 kcal. This nutrient density approach identified qualitative patterns of food consumption for selected income, region, urbanization, household size, race, employment and education groups as well as …

Associative Growth Of Paired Lactic Streptococci In Ph Controlled Whey-Based Media, Carol Lee Hansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study evaluated four lactic streptococcal pairs which had been used routinely in cheese plants to determine (1) if strain growth interactions would produce stimulated growth and acid production; (2) final percent strain ratios and generation times after 1/1 initial inocula volumes and (3) whether final strain ratios could be controlled through adjustment of initial inocula volumes. Strains were propagated in fortified and unmodified whey substrate under pH control at 27C for 12 hours. Cell number and acid activity were evaluated on single and paired strains. Phage typing was used to determine generation times and the percent of each strain …

Seasonal Variation In The Ability Of Milk And Whey To Support Lactic Culture Growth, Rick Cameron Norton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Milk samples from two cheese plants with overlapping milk supplies were collected monthly for one year in an attempt to measure seasonal variation in the ability of milk and whey to support lactic culture growth . Treatments to control and minimize variability of milk or whey were evaluated to optimize stability in starter culture performance.

Raw milk samples were tested for somatic cell counts, activity tests (modified Horrall- Elliker), acid degree values, and total plate counts. Activity (modified Horraii-EIIiker) and inhibitory tests were also performed on pasteurized, pasteurized-vacuumized and high heat milk treatments.

Rennet whey (heated and unheated) was collected …

Assay, Purification, And Characterization Of A Pantetheine Hydrolyzing Enzyme From Pig Kidney, Carl Thomas Wittwer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A microsomal glycoprotein hydrolyzing pantetheine to pantothenate and cysteamine has been solubilized and purified to homogeneity as determined by sodium dodecylsulfate electrophoresis. Four rapid, independent assays of pantetheine hydrolysis are described and compared along with a method for localizing enzymatic activity on polyacrylamide gels. The enzyme is solubilized on exposure to butanol and purified by heat treatment, (NH4)₂SO₄ fractionation, hydrophobic chromatography, and hydroxyapatite chromatography. The glycoprotein, purified 5600-fold in 22% yield, has a specific activity of 14 μmoles pantothenate produced/min/mg of protein, 35 times that previously reported. The enzyme has a pH optimum of 9.0-9.5 and a …

An Evaluation Of Deschampsia Caespitosa (L.) Beauv Populations For Metal Tolerances And Ecotypic Variation At Climax, Colorado, Paul Ellsworth Pratt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Four populations of Deschampsia caespitosa (L.) Beauv (a Poaceae species), growing on disturbed and undisturbed sites at Climax, Colorado, were examined for metal tolerances (Zn, Cu, Pb, Al). Root growth assessments on parent plant and offspring material for each population were conducted using nutrient solutions containing elevated levels of Zn, Cu, Pb, or Al. Seed germination and seedling survival tests for each population were conducted on both alpine topsoil and mine spoil material.

Evidence is presented that supports the hypothesis that ecotypic variation exists within Deschampsia caespitosa and that this variation has allowed certain Deschampsia populations to become successfully established …

The Annual Meeting Of The Iowa Academy Of Science April 16-17, 1982 [Program, 94th Meeting], Iowa Academy Of Science

Iowa Academy of Science Documents

No abstract provided.

Abstracts Of Contributed Papers, 94th Session, Iowa Academy Of Science, April 16-17, 1982, Iowa Academy Of Science

Iowa Academy of Science Documents

Presentation abstracts from the annual meeting of the Iowa Academy of Science

Press Water Reclamation Using A New High Solids Filter, Jonathan C. Kerr

Paper Engineering Senior Theses

Press section water cannot be reused on a fine papermachine due solely to the fact that fibrous contaminants, specifically felt fibers that are removed by the felt cleaning system, cause an inordinate amount of trailing blade coater scratches and other defects in the final product. In this study, the feasability was examined of using a Ronnigen-Petter CycloSpray high solids filter to remove felt and cellulose fibers from press water.

By maintaining a constant fiber content of 0.4 lbs./1000 gallons and increasing the filler loading from 20 lbs./1000 gallons to 40 lbs./1000 gallons, the effect of increasing filler loading on fiber …

Sunset As An Orientational Cue For A Nocturnal Migrant, The White-Throated Sparrow (Zonotrichia Albicollis), James Pauly

Masters Theses & Specialist Projects

The possibility that nocturnal migrants use sunset as an orientational cue was explored in the White-throated Sparrow (Zonotrichia albicollis) between 1 April and 24 April, 1981. Orientation tests were run on the roof of the biology building at Western Kentucky University when birds were assumed to be in the proper physiological condition for migration. Birds exposed to only nighttime skies, birds isolated from all visual cues, and birds exposed to both daytime and nighttime skies did not exhibit the northward directional preference appropriate for the season tested. However, White-throated Sparrows exposed only to sunset and tested in the absence …

Differences Associated With Mating Type Alleles In Myxomycetes, Robert M. Queen

Retrospective Theses and Dissertations

Differences associated with the mating type alleles of Didymium iridis and Physarum polycephalum were examined with fluorescent antibody and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Heteroabsorption of each anti-myxamoebal serum followed by testing serum activity using immunofluorescence showed there is no strain-specific activity in any of the anti-myxamoebal sera, but the sera was shown, to be genera specific. Intergeneric differences and similarities were shown in the electrophoretic patterns of the myxamoebal protein extracts from P. polycephalum, D. iridis, and Dictyostelium discoideum when compared. Intraspecific differences were noted in D. iridis.

Louisiana Agriculture Magazine, Spring 1982, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.