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Characteristics Of Binding Of Interleukin-1 To Yt.Nci Cells, Susan Catherine Speziale

Masters Theses

Interleukin-1 (IL-1) demonstrated a concentration dependent, specific binding to the human, large granular lymphocyte cell line, YT.NCI with a dissociation constant of 0.2 nM. There were approximately 500 IL-1 receptors/cell.

IL-1 binding to YT.NCI cells could be inhibited by unlabeled-IL-1 and the plant lectins, WGA and Con A. Lectin inhibition of IL-1 binding could be completely abrogated by exposing lectins to specific simple sugars prior to the addition of ligand.

Tunicamycin, an inhibitor of glycosylation, decreased IL-l binding to YT cells in a reversible, time- and concentration-dependent manner.

These observations suggest that glycosylation of cell surface proteins may be important …

Use Of 52Cr For The Quantitative Determination Of Red Cell Volume, Claude Michael Hanbury

Chemistry & Biochemistry Theses & Dissertations

Chromium-52 has recently been suggested for use as a new agent to label red cells for the in vivo quantitation of red cell volume (1). In this paper, the development and validation of a routine ⁵²cr labeling procedure is described.

The accuracy, precision, and detection limits of chromium analysis by Zeeman effect atomic absorption spectroscopy was evaluated in the concentration range of 1 - 10 ug Cr/L.

Red cell chromium uptake was evaluated as a function of time, temperature, and concentration. Red cells labeled with a 2.5 mg/L chromium solution for 30 minutes at room temperature exhibited optimal label …

The Relationship Between Multiple Health Factors, Demographics, And Body Weight, Glenda J. Potter

Community & Environmental Health Theses & Dissertations

The purpose of this study was to test the relationship between regular aerobic exercise and body weight. It was the intent of this study to determine if a person who regularly exercises at either high or moderate levels can maintain their body weight at desired levels as specified by the Body Mass Index (BMI). The participants in this study were selected from hospital employees, class members of aerobic classes and/or adult walkers/joggers. There were 38 participants-- 13 males and 25 females.

A self administered questionnaire and a 3-day, 24-hour dietary log were identified to comprise the instrument as well as …

Effects Of Ethanol And Caffeine Administered Maternally On Neonatal Adrenocortical Function, Rhonda L. Mullins Rohlfing

Biological Sciences Theses & Dissertations

Two investigations were conducted to determine the effects of maternally administered ethanol and caffeine on neonatal adrenocortical function. In the ethanol study, pregnant female rats were fed a liquid diet in which 35% of the sucrose calories were removed and replaced with ethanol. Dams in the caffeine study were injected with 25 mg/kg/day caffeine in saline solution. Within 24 hours of parturition, neonatal rats in both studies were sacrificed. The adrenal glands were incubated in vitro for one hour and media were analyzed for corticosterone content. Significant adrenal weight differences were found in both studies. The ethanol group pups were …

Developmental And Radiobiologic Characteristics Of Canine Multinucleated, Osteoclast-Like Cells Generated In Vitro From Canine Bone Marrow, T. M. Seed, L. V. Kaspar, F. Domann, G. K. Niiro, D. A. Lebuis

Scanning Microscopy

We report here our initial observations on the growth and morphology, and developmental radiosensitivity of giant, multinucleated, osteoclast-like cells (MN-OS} generated through in vitro cultivation of hematopoietic progenitor-enriched canine bone marrow samples. Maximum cell densities of 5.5 x 10³ to 6.5 x 10³ MN-OS per cm² of growth area were achieved following 10 to 14 days of culture at 37°C. Acute gamma irradiation of the initial marrow inocula resulted in significant, dose-dependent perturbations of MN-OS formation, growth, and development. Attempts to estimate radiosensitivity of MN-OS progenitors from canine marrow yielded a range of D_O values from …

Dna Probe Method For The Detection Of Specific Microorganisms In The Soil Bacterial Community, William Holben, Janet K. Jansson, Barry K. Chelm, James M. Tiedje

Biological Sciences Faculty Publications

We developed a protocol which yields purified bacterial DNA from the soil bacterial community. The bacteria were first dispersed and separated from soil particles in the presence of polyvinylpolypyrrolidone, which removes humic acid contaminants by adsorption to this insoluble polymer. The soil bacteria were then collected by centrifugation and lysed by using a comprehensive protocol designed to maximize disruption of the various types of bacteria present. Total bacterial DNA was purified from the cell lysate and remaining soil contaminants by using equilibrium density gradients. The isolated DNA was essentially pure as determined by UV spectral analysis, was at least 48 …

Sfa Gardens Newsletter, Mar 1988, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Newsletters

No abstract provided.

A Decade Of Use Of Livestock Guarding Dogs, Raymond Coppinger, Lorna Coppinger, Gail Langeloh, Lori Gettler, Jay Lorenz

Vertebrate Pest Conference Proceedings: 13th (1988)

Results from a ten-year study of livestock guarding dogs show that the dogs are an effective tool for reducing predation. Average reduction attained by five strains of dogs (Anatolian Shepherds, Maremmas, Shar Planinetz, Anatolian/ Shars, Maremma/Shars) was 64%, with predation reduced to zero for 53% of reporting producers in 1986. Variations in trustworthy, attentive and protective behavior of the dogs were breed-specific, and offer mechanisms for improving the system.

Solute Exclusion By Polymer And Protein-Dominated Water: Correlation With Results Of Nuclear Magnetic Resonance (Nmr) And Calorimetric Studies And Their Significance For The Understanding Of The Physical State Of Water In Living Cells, G. N. Ling

Scanning Microscopy

According to the polarized multilayer (PM) theory of cell water proteins with their back-bones fully extended and their NHCO groups directly exposed to bulk water, polarize water in multilayers. Experimental testing of the theory led to a new understanding of the uniqueness of gelatin, due to its permanently maintained fully extended conformation and its ability to polarize the bulk phase water in multilayers with reduced solubilities for solutes in a size dependent manner ("size rule"). Other models which behave like gelatin are urea-denatured proteins, synthetic polymers like polyethylene oxide (PEO), and polyvinylpyrrolidine (PVP), but not native proteins. NMR studies showed …

Clinical Applications Of Scanning Electron Microscopy And Energy Dispersive X-Ray Analysis In Dermatology - An Up-Date, Bo Forslind

Scanning Microscopy

Dermatological papers comprising scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis data published 1983 through 1986 in international journals are reviewed, as an update to our 1984 paper on Clinical applications of scanning electron microscopy and X-ray microanalysis in dermatology. The present paper not only deals with a review of recent publications in this area but also presents the application of microincineration to hair and cryosectioned freeze-dried skin specimens. Examples of the increased contrast obtained in hair cross sections are presented and a discussion on the feasibility of microincineration at analysis of hair and skin cross sections is …

A Tool For Integrating Conceptual Schemas And User Views, Amit P. Sheth, James A. Larson, Aloysius Cornelio, Shamkant B. Navathe

Kno.e.sis Publications

An interactive tool has been developed to assist database designers and administrators (DDA) in integrating schemas. It collects the information required for integration from a DDA, performs essential bookkeeping, and integrates schemas according to the semantics provided. The authors present the capabilities of this tool by discussing the integration methodology and the user interface of the tool.

Policy For Identifying And Assessing The Health Risks Of Toxic Substances, Maine Bureau Of Health

Maine Collection

Policy for Identifying and Assessing the Health Risks of Toxic Substances

by Norman T. Anderson, Environmental Toxicology Program, Division of Disease Control, Bureau of Health (February, 1988).

Contents: Section I: Introduction / Section II: Methods / Section III: Exposure Assessment / Section IV: Hazard Identification / Section V: Hazard Assessment / Section VI: Risk Characterization / List of Figures / List of Tables

Photographic Technique For Grass Seedling Root Systems, P. R. Newman, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

In descriptive studies of seedling root morphology a seedling root system must be clearly visible to develop clear photographs. A technique for photographing grass seedling root systems has been developed for morphological-developmental studies. Seedlings were carefully washed form the growing media and placed in a thin tank with two glass sides filled with water and with a grid pattern on one side. The lower stem and root system were immersed in the water where the natural buoyancy of the root system in water allowed the root to spread out, which improved visibility. Photographs were taken of the illuminated seedling root …

Comparative Adsorption Studies On Clay Soils, Prasanna R. Mysore

Theses

An investigation has been made to study clay as sorbent in the treatment of the hazardous contaminants of potential sludge leachate emanating from industrial landfills.

Experimentally was studied six highly toxic and hazardous chemicals, which include: phenol, aniline, cyclohexanol, cyclohexanone, cresol and monochlorophenol. Vermiculate, Kaolinite, Attacote and Hectorite clays were studied as the sorbent material. Removal of organic pollutants were measured using a total organic carbon analyzer (TOC).

Results indicate that these clays can be used for some treatment of the above mentioned organic compounds. The study shows that removal of organic pollutants by clay depends on several factors, such …

Perspectives On Process In Plant Virology, Myron K. Brakke

Department of Plant Pathology: Faculty Publications

The editors asked that I write a chapter on landmarks in plant virology, a topic that has been covered by several authors, for example by Henderson Smith (89), Bawden (9), Holmes (51), Markham (62), Harrison (44), Black (12), and Matthews (64). In 1 938, the first of these authors, Henderson Smith (89), divided his presidential address to the Society for Applied Biology between the control of plant-virus diseases and the nature of plant viruses. Progress since then has been much more rapid in the latter than in the former area. Henderson Smith could easily understand today's literature on losses, control, …

The Potentate Of P.I.O. : [Judith Schwartz], Marc Kaplan, Deborah Edelman

Rockefeller University Research Profiles

Rockefeller University Research Profiles are a series of scientific profiles that were published quarterly, from 1980-1990, by the Rockefeller University. Each issue features the research and achievements of an individual Rockefeller University scientist.

The Itasca State Park Algal Culture Collection At Loras College, David B. Czarnecki, M. Jon Ross

Journal of the Minnesota Academy of Science

A list of over 125 algal cultures originating from Lake Itasca State Park is presented. These cultures, permanently housed at Loras College, Dubuque, Ia., include 115 taxa representing 68 genera from six algal Divisions. Approximately one-third of the taxa are desmids.

Environmental, Genotypic, And Ploidy Effects On Endopolyploidization Within A Genotype Of Solanum Phureja And Its Derivatives, Henry R. Owen, R. E. Veilleux, D. Levy, D. L. Ochs

Faculty Research & Creative Activity

Flow-cytometric analyses of DNA content were performed on chopped in vivo, in vitro, and protoplast-derived in vitro nuclei of Solanum phureja. An anther-derived monoploid genotype and a diploid and tetraploid clone, derived from callus culture of the monoploid genotype, were used to characterize the influence of in vivo and in vitro environment and explant ploidy level on the extent of endopolyploidization. In addition, protoplast-derived nuclei, from nine anther-derived monoploid genotypes, were examined for genotypic influences on endopolyploidization. DNA distributions of the anther-derived monoploid and callus-derived clones in vivo contained peaks corresponding to 1C, 2C, and 4C DNA levels. By comparison, …

The Physiological Implications Of Bleaching Of Corals Off Southeast Florida, C. J. Reese, G. S. Kleppel, Richard E. Dodge

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

As part of an ongoing study of coral bleaching, observations were made of corals on reefs (ca. 8m) off John U. Lloyd Beach, Dania, FL, in December, 1987. Bleaching was evident in approximately 50% of Montastrea annularis, the dominant scleractinian coral species. Quantitative pigment measurements by HPLC show that bleached corals contained

Decline Of Coral Growth Rates At Negril, Jamaica, T. J. Goreau, Richard E. Dodge, P. D. Goreau

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Montastrea annularis heads were collected near Negril, Jamaica, along transects from open, normal reef conditions into the mouths of rivers draining the Negril Morass. Corals subject to turbidity (particulate peat and dissolved humic compouonds near rivers, re-suspended reef sediments away from them) were growing more slowly than those in normal circumstances. Surprisingly, corals growing fastest were slowing the most, and the slowest growers increasing growth rate. Linear regression analysis of growth trends over the past two decades revealed stable limit-cycle behavior: change of growth was inversely proportional to growth rate, with a very significant correlation coefficient of -0.92. We suggest …

Ua35/11 Wku Student Honors Research Bulletin, Wku University Honors Program

WKU Administration Documents

The Western Kentucky University Student Honors Research Bulletin is dedicated to scholarly involvement and student research. These papers represent work done by students from throughout the university.

• Kesselring, Marcia. Attitudes Toward the Need for Computer Literacy
• Tuck, Janna & Karen Wiggins. Methylation and Confirmation of PGE
• Lewis, Gloria. John Donne's Attitude Toward Love
• Johnson, Linda. International Telecommunications Trade with Japan
• Sharpe, Greg. Precipitation Patterns in Bowling Green, Kentucky, 1980-1985
• Smith, Sandy. Religion and the Media: Alliance or War?
• Bell, Suzanne. Early Secret Involvement of the United States Military in Cambodia
• Scariot, Linda. Parental Divorce and Childhood Emotional Disturbances
• Daniel, Janice. …

Plans Of Action For Southeast District Issues 1988 - 1992

Southeast Research and Extension Center: Reports and Administration

The Southeast Research and Extension District Alternative Agricultural Opportunities Team met on Thursday, September 15th and reviewed the state plan for the initiative "Increasing Diversity with Alternate Crop and Livestock Enterprises".

The team members present decided that the state plan would meet the planning needs for the Southeast District if it was supplemented with a list of enterprises which have the greatest potential for additional expansion in Southeast Nebraska.

The following alternate crop and livestock enterprises were identified by the committee as having the best opportunity for additional development in the district. In crops; asparagus, melons, and onions were identified …

Spermiogenesis Of Eupyrene Sperm In Prepupae"} Pupae"} And Adults Of Heliothis Virescens (Lepidoptera: Noctuidae): An Ultrastructural Study, Leo E. Lachance, Geneva Olstad

Entomology Papers from Other Sources

We have examined the ultrastructure of post meiotic eupyrene sperm maturation in testes of prepupae, pupae, and adult Heliothis virescens (F.) males. Emphasis was placed on the structures present in the elongating sperm tail. In each young spermatid, each cell has a nucleus, acrosome derivative, and numerous mitochondria in a large volume of cytoplasm. These mitochondria coalesce to form a body, the nebenkern. As the sperm cell begins to elongate, the nebenkern divides into two mitochondrial derivatives (MDs) of unequal size. The MDs have an outer and inner membrane, and as they elongate and coil in the sperm tail, cristae …

Descriptions Of Pupae Of South American Robber Flies (Diptera: Asilidae), D. Steve Dennisi, Lloyd Knutson

Entomology Papers from Other Sources

The pupae of four South American species of robber flies, Diogmites vulgaris Carrera, Mallophora ruficauda (Wiedemann), Mallophora sylverii Macquart, and Triorla striola (F.), are described and illustrated. The pupae of these species are compared morphologically with those of North American species of the same genera. A key to pupae of Asilidae at the subfamily level is provided. Biological notes associated with the specimens examined and with those of Pseudorus distendens (Wiedemann) indicate that the larvae prey upon immature stages of Scarabaeidae (Dyscinetus rugifrons LeConte for D. vulgaris and M. sylverii, Cyclocephala signaticollis Burmeister for M. ruficauda, and a …

Annual Report Of The Iowa Academy Of Science, 1987-88, James G. Macmillan

Journal of the Iowa Academy of Science: JIAS

Since 1975 when a history of the Iowa Academy of Science was written for the centennial of the founding of the Academy, an Annual Report has acted as a continuing record of the Academy’s activities.

This report encompasses the year beginning July I, 1987 to June 30, 1988 and is based on minutes of committee meetings, Board minutes and recollections of the author. Each part represents a major activity of the Academy.

The Mrna Cap And Discontinuous Transcription In Trypansoma Brucei, Marion Struik Freistadt

Student Theses and Dissertations

The protozoan parasite, Trypanosoma brucei, and other members of the phylogenetic order, Kinetoplastida, synthesize mRNA by a novel pathway that joins RNAs from physically unlinked gene segments. This results in the presence of a short non-coding RNA leader, called the miniexon, at the 5' end of most, probably all, mRNA. Indirect evidence suggests that trans-splicing, rather than RNA priming of transcription, is the mechanism by which the mini-exon is joined to RNA containing protein-coding sequences. In vivo ³²P-labeled RNA was purified by hybrid selection with mini-exon genomic DNA, in order to analyze the structure of medRNA, the 140- nucleotide …

Ultrastructural And Textural Properties Of Restructured Beef Treated With A Bacterial Culture And Splenic Pulp, E. A. Elkhalifa, N. G. Marriot, R. L. Grayson, P. P. Graham, S. K. Perkins

Food Structure

Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Instron measurements were used to evaluate the effects of an Achromobacter iophagus culture {BC) and splenic pulp (SP) treatments one structural and textural properties of flaked and restructured beef steaks. Both treatments improved the textural character is tics of the product when conditioned at 35°C . Electron microscopy studies revealed that the bacterial culture treatment caused a greater effect than SP on the connective tissue elements, with a degradation of the endomysial sheath and sarcolemma. Treatment with splenic pulp produced an overall excessive disruption a t the Z-lines with little definition …

Application Of Scanning Electron Microscopy And X-Ray Microanalysis To Investigate Corrosion Problems In Plain Tinplate Food Cans And Examine Glass And Glass-Like Particles Found In Canned Food, James E. Charbonneau

Food Structure

Scanning Electron Microscopy (SEM) and X-ray Microanalysis (EDS) have been used to investigate product container interaction problems in plain tinplate metal food containers and to examine glass and glass-like particles that were found in canned food.

Through the use of SEM-EDS, it was determined that: sulfate containing particles from the cannery cooling water reacted with exposed iron or tin-iron alloy on the tinplate surface causing an external rusting problem; sulfur dioxide was responsible for a container discoloration and a pitting corrosion problem in canned fruit nectar; hydrogen sulfide produced from the SO2-tinplate reaction was the cause of an associated off-odor …

Effect Of Biotin Limitation On The Conversion Of Xylose To Ethanol And Xylitol By Pachysolen Tannophilus And Candida Guilliermondii, Hung Lee, Audrey L. Atkin, Maria F.S. Barbosa, Delbert R. Dorscheid, Henry Schneider

School of Biological Sciences: Faculty Publications

The relative amount of ethanol and xylitol accumulated in aerobic batch cultures of Pachysolen tannophilus and Candida guilliermondii on D-xylose depended on the extent of limitation by biotin. In high biotin media P. tannophilus favored ethanol production over that of xylitol while C. guilliermondii favored xylitol formation. However, as the extent of biotin limitation increased, the ratio of ethanol to xylitol produced by both organisms increased. The results are of interest in efforts to control such ratios.

Changes In The Microstructure Of Saint Paulin Cheese During Manufacture Studied By Scanning Electron Microscopy, Micheline Rousseau

Food Structure

The Saint Paulin cheese used in this study contains 45% fat. Changes in curd structure were followed during syneresis until the end of ripening. Two scanning electron microscopy (SEM) techniques were used: the conventional technique with sample fracture after critical point drying, and cold-stage SEM after rapidly freezing samples at -160 C. The two techniques are complementary since the first leads to satisfactory observation of casein, while the other is more suitable for observing fat globules.