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The Ultrastructure Of Dentine From Rat Incisors Following Exposure To Sodium Fluoride And Potassium Pyroantimonate Staining, J. Appleton

Scanning Microscopy

Weanling rats were given a single intra-peritoneal injection of sodium fluoride and control animals normal saline for four consecutive days. The fluoride produced a consistent response in the mineralizing dentine of the incisors in which a hypermineralized band was succeeded by a hypomineralized band. Potassium pyroantimonate staining for calcium ions showed that following injection of fluoride, in contrast to the controls, there were large amounts of calcium pyroantimonate in the pre-dentine and throughout the odontoblasts. This suggests that fluoride temporarily affects the membrane enzyme systems which maintain calcium concentration gradients between the odontoblasts and the matrix. The resultant influx of …

Report And Planning Meeting, Various Authors

Other Documents

No abstract provided.

Comparison Of The Calcium Distribution Pattern Among Several Kinds Of Hard Tissue Forming Cells Of Some Living Vertebrates, Yasutoku Kogaya, Kuhei Furuhashi

Scanning Microscopy

We investigated the ultrastructural distribution of calcium in several kinds of hard tissue forming cells (secretory and maturation ameloblasts, odontoblasts osteoblasts, chondrocytes, and osteodentine forming cells) of mammals, amphibians, and fish by use of the potassium pyroantimonate technique. The calcium distribution pattern is compared among these cells, and its biological significance is discussed. Except for mammalian odontoblasts, all types of the hard tissue forming cells exhibited fundamentally the same distribution pattern of calcium; the antimonate reaction product was mainly localized on the inner face of the plasmalemma and inside mitochondria. On the other hand, in mammalian odontoblasts, the reaction product …

Effect Of Fluoride And Cobalt On Forming Enamel: Scanning Electron Microscope And X-Ray Microanalysis Study, S. H. Ashrafi, D. R. Eisenmann, A. E. Zaki, R. Liss

Scanning Microscopy

The forming surfaces of enamel of rat incisors were examined by scanning electron microscope one hour after injection of either 5 mg/100 g body weight of sodium fluoride or 12 mg/100 g body weight of cobalt chloride. The cell debris from the surfaces of the separated incisors was either gently wiped off with soft facial tissues or chemically removed by treating with NaOH, NaOCl or trypsin. Best results to remove cell debris were obtained from 0.25% trypsin treatment.

SEM studies revealed that the surface of the normal secretory enamel was characteristic in appearance with well-developed smooth prism outlines. In fluoride …

Mounting Materials For Automated Image Analysis Of Coals Using Backscattered Electron Imaging, W. E. Straszheim, K. A. Younkin, R. T. Greer, R. Markuszewski

Scanning Microscopy

In order to apply SEM-based automated image analysis (AIA) to the characterization of not only minerals in coal but to the coal itself, sample preparation methods need to be developed beyond common practice. A significant consideration is the degree of contrast achievable between the mount media chosen and the coal. Four low-atomic number materials (epoxy, polyethylene, polystyrene and carnauba wax) were compared for their potential as suitable mounting media for coal samples. Epoxy is satisfactory only for characterization of mineral particles since the contrast between epoxy and coal particles is negligible. Polyethylene or polystyrene have marginal application for use as …

The Use Of Backscattered Electron Imaging, X-Ray Microanalysis And X-Ray Microscopy In Demonstrating Physiological Cell Death, I. D. Bowen, N. A. Worrill, C. A. Winters, K. Mullarkey

Scanning Microscopy

The cytochemical localization of enzymatic activity by means of backscattered electron imaging (BEi) is reviewed and the application of BEI to changes in acid phosphatase and ATPase distribution during physiological (programmed) cell death in Heliothis midgut is explored. Programmed cell death entails the release of nascent free acid phosphatase as extracisternal hydrolase. This shift can readily be detected by means of the atomic number contrast imparted by BEI of the lead phosphatase reaction product, thus enabling the distribution of dying cells to be mapped. BEI is particularly useful in this context as it allows the examination of bulk specimens at …

Isolation And Characterization Of Monoclonal Antibodies Directed Against Plant Plasma Membrane And Cell Wall Epitopes: Identification Of A Monoclonal Antibody That Recognizes Extensin And Analysis Of The Process Of Epitope Biosynthesis In Plant Tissues And Cell Cultures, David J. Meyer, Claudio L. Alfonso, David W. Galbraith

Papers in Microbiology

Membranes from tobacco cell suspension cultures were used as antigens for the preparation of monoclonal antibodies. Use of solid phase and indirect immunofluorescence assays led to the identification of hybridomas producing antibodies directed against cell surface epitopes. One of these monoclonal antibodies (ll.D2) was found to recognize a molecular species which on two-dimensional analysis (using nonequilibrium pH-gradient electrophoresis and SDS-PAGE) was found to have a high and polydisperse molecular mass and a very basic isoelectric point. This component was conspicuously labeled by [3H]proline in vivo. The monoclonal antibody cross-reacted with authentic tomato extensin, but not with potato lectin nor larch …

Modulation Of Queuine Uptake In Cultured Human Fibroblasts By Phorbol Esters And Interferons, Debra L. Crane

Chemistry & Biochemistry Theses & Dissertations

Queuosine, a nucleoside found exclusively in the first position of the anticodon of transfer RNA (tRNA), is generated post-transcriptionally in an exchange of guanine for queuine by the modifying enzyme tRNA-guanine ribosyltransferase. Phorbol ester tumor promoters were shown to induce queuine hypomodified tRNA, and that phorbol ester action was due, in part, to inhibition of queuine transport across the cell membrane. An unidentified protein designated conditioned medium factor (CMF) that potentiated a phorbol-induced inhibition of queuine uptake was also documented. We suggest here that phorbol ester-induced inhibition of queuine uptake is not a significant factor in inducing queuine-deficient tRNA although …

Agricultural Research Division 102nd Annual Report, July 1, 1987, To June 30, 1988

Agricultural Research Division: News and Annual Reports

It is a pleasure to provide the 102nd Annual Report of the UNL Agricultural Research Division. This report contains lists of current faculty, active projects, referred journal articles, books and book chapters, germplasm releases, patents, brief descriptions of research in selected areas, and the financial report for the period of July 1, 1987, through June 30, 1988. This report was compiled in compliance with the intent of the law of the State of Nebraska that established the Nebraska Agricultural Experiment Station on March 31, 1887.

Foreword ... v

Administrative Personnel ... vii

Administrative Units ... viii

Organizational Chart ... viii …

Topographic Evolution In The Atomic Scale Growth And Erosion Continuum, G. Carter, I. V. Katardjiev, M. J. Nobes, J. L. Whitton

Scanning Microscopy

This review gives a detailed survey of the range of fascinating surface features which develop under growth or erosion conditions under the combined influence of thermal and more energetic atomic particle fluxes. Collisionally induced atomic ejection and migration, and thermally and radiation induced atom and defect diffusion processes are outlined and their relevance to topographic initiation and evolution explored. A range of experimental observations of surface feature elaboration is discussed from net growth to net erosion conditions and models for their explanation are considered. It is concluded that while much data have been accumulated, much of these have been in …

Agenda: Water Quality Control: Integrating Beneficial Use And Environmental Protection, University Of Colorado Boulder. Natural Resources Law Center

Water Quality Control: Integrating Beneficial Use and Environmental Protection (Summer Conference, June 1-3)

Conference organizers and/or faculty included University of Colorado School of Law professors David H. Getches, Lawrence J. MacDonnell and Charles F. Wilkinson.

Protecting water quality is essential to preserve the many beneficial uses of western water resources. This conference addresses the dominant federal requirements in the Clean Water Act, including the important major revisions enacted by Congress in 1987, with special attention to western problems regarding nonpoint source pollution. Developments in groundwater quality regulation are considered, as are selected issues concerning the implications of state and federal water quality regulation for the traditional exercise of water rights.

Immunologic Mechanisms Involved With Human Endodontic Lesions, Sunita V. Prabhu

Loma Linda University Electronic Theses, Dissertations & Projects

Endodontic or root canal infection results in immunologic changes in periapical region, which causes the destruction of pulpal and periapical tissue. No definitive study has identified a specific organism or a group of organisms as a etiological agents. Past studies have indicated that immunoglobulin E play a significant role in the pathogenesis of periapical lesions. The presence of this factor suggests that some of the endodontic flare-ups may be IgE-mediated hypersensitivity reactions. Antibody levels of IgA have been quantitatively measured by ELISA and IgE by modified RAST assay, against eleven oral bacteria which are routinely isolated from such lesions. The …

Determination Of Antibody Levels In Periapical Lesions Against Sixteen Oral Microorganisms By The Elisa Technique, Sammee Lee Jones

Loma Linda University Electronic Theses, Dissertations & Projects

The root canal system, which includes the periapical region of the tooth, may become infected by several types of microorganisms which are normally associated with these tissues. A bacteria-specific antibody response may occur at the local level, where various classes of immunoglobulins have been found and reported in the literature. The purpose of this study was to quantitatively measure locally produced immunoglobulin' levels in periapical lesion abscess materials and to determine the specific reactivity of these antibodies to several microorganisms which are normal flora, as well as being previously associated with the development and pathogenesis of endodontic infections. Periapical lesions …

A New Hazardous Waste Treatment Technology Utilizing Low Power Density Microwave Energy, Gabriele Else Windgasse

Theses

Two major applications of a new hazardous waste treatment technology using low power density microwave energy have been characterized: 1) Desorption of organic materials such as: trichloro-ethylene, para-xylene, naphthalene and gasoline hydrocarbons from substrates such as: sand and granulated activated carbon was achieved by microwave induced steam distillation and the application of a new phenomenon: arcing between GAC particles when they are exposed to microwave radiation. All contaminants could be removed to 100% (non-detectable with GC and GC-MS). 2) Decomposition of organic contaminants such as: trichloro-ethylene and trichloro-ethane in arcing/heating GAC beds. A new fluidized bed reactor ("RDW-reactor", after Ray, …

Polarized Epithelial Cysts In Vitro: A Review Of Cell And Explant Culture Systems That Exhibit Epithelial Cyst Formation, J. A. Mcateer, G. S. Dougherty, K. D. Gardner Jr., A. P. Evan

Scanning Microscopy

The purpose of this paper is to review in vitro cell and explant culture systems that exhibit epithelial cyst formation and that are used as models of polarized epithelial function. We examine a number of culture systems derived from a variety of cell and organ types, briefly describe the methodology and conditions used to establish these cultures and discuss aspects of the experimental application of each system. We conclude that the characteristics of epithelial cyst-forming cultures are dependent upon the origin and identity of the cell population, as well as the multiple factors that define the culture environment. Culture systems …

Preparation Of Cultured And Isolated Cells For X-Ray Microanalysis, K. Zierold, D. Schäfer

Scanning Microscopy

Various electron microscopical preparation techniques are compared with regard to the preservation of the intracellular element distribution as determined by X-ray microanalysis in scanning and scanning transmission electron microscopy. By use of chemical agents for fixation and dehydration ions are redistributed and washed out. This is also true for freeze-substitution. Whole cells are prepared by cryofixation followed by freeze-drying. Interference of intracellular measurements by extracellular elements can be avoided by appropriate washing the cells before cryofixation. The washing medium has to be carefully selected in order to avoid distortions of the original intracellular element content. These problems are circumvented by …

Intracellular Water Distribution And Aging As Examined By X-Ray Microanalysis, Thomas Von Zglinicki

Scanning Microscopy

The results reviewed here demonstrate that 1. the distribution of dry mass as observed in frozen-dried cryosections might be used as an unbiased measure of intracellular dry mass resp. water distributions in the tissue in vivo and 2. the well-known loss of water from cells during aging is solely due to a water loss from mitochondria without changes in the water content of all other components of the cell in the case of rat liver and heart muscle.

The reason for the water loss might be increased counter ion binding by membrane-bound enzymes due to decreased fluidity of the inner …

Characterization Of Chemical And Radiation-Induced Dna Damage In The Mdbk Cell Culture System, Jeffrey R. Hincks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A mechanism common to many carcinogens and mutagens is the interaction with cellular DNA. Alkaline elution is a sensitive and commonly used technique to detect cellular DNA damage in the form of DNA single-strand breaks (SSB), DNA-protein cross-links (DPC), and DNA interstrand cross-links (ISC). In the present study, gravity-flow alkaline elution was developed and validated to rapidly detect DNA damage in the MDBK cell culture system by genotoxic agents.

The system was first validated by detecting a dose dependent induction of DNA SSB in cells exposed to 25-1500 rad of X-ray. The assay reliably detected the DNA damage of seven …

Some Chemical And Physical Factors Influencing Growth Of Panus Stipticus Bulliard Ex Fries And Armillariella Mellea (Vahl Ex Fries) Karsten, James Robert Kirkland

Master's Theses

Two species of wood rotting fungi were used to investigate the possibility that growth-enhancing or inhibitory substances in woods from various trees are responsible for the distribution of these fungi in nature. Panus stipticus (Bulliard ex Fries) and Armillariella mellea (Vahl ex Fries) Karsten were cultured in the laboratory on blocks of wood from Taxodium distichum (L.) Richard, Juniperus virginiana L., Pinus taeda L. and Quercus rubra L. Panus stipticus grew well on the surface of the wood blocks; histological studies showed less penetration into the xylem of the softwoods than into that of Q. rubra. Armillariella mellea grew well …

Analyses Of Somaclonal Variation In Hexaploid Wheat (Triticum Aestivum L.), Zahra Noori Hashim

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Somaclonal variation, to provide germplasm for crop improvement, must be screened, selected and characterized. Immature wheat, Triticum aestivum L., (PCYT-10) embryos (10-12 days old) were cultured on Hurashige and Skoog (MS) medium containing 0.5 mg L^-1 6-furfurylaminopurine (kinetin) and 2, 3, or 4 mg L^-1 of 1-methoxy-3,6-dichlorobenzoic acid (2,4-D). Dicamba, at 2 and 3 mg L^-1 and 0.2 mg L^-1 2,4-D, produced 12.7%, 30.3%, and 28.2% of the somaclones, respectively. No plantlets were produced from other treatments. Variants were characterized by cytology, biochemistry and morphology. Somaclones showed significant differences in length and width of flag leaf, …

Evaluation Of Frogurt: A New Product Prepared By Fermentation Of Ice Cream Mix, Morteza Mashayekh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ice cream mix was fermented with yogurt cultures of Streptococcus thermophilus and Lactobacillus bulgaricus to four different pH's then frozen in a batch ice cream freezer. A consumer panel of 120 people tasted samples of strawberry flavored product with pH's of 4.4, 4.7, 5.1, and 5.4 and commercial frozen yogurt as a standard. Results from the panel were used to predict a preferred pH of 4.9. Another panel of 181 people compared product at pH 4.9 with 10, 15 and 20% strawberry flavoring. There was not a statistically significant difference among levels of flavoring.

Starter culture populations and lactase activity …

Studies On Encephalomyocarditis Virus-Induced Diabetes In Mice, John Henry Huffman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The D-variant of encephalomyocarditis (EMC-D) virus was given to SJL/J mice by the artificial, intraperitoneal (ip) route of infection, or by the natural routes of infection per os (po) or intranasal (i.n.), in comparable concentrations. The po route of infection was found to be ineffective. Mice given virus by either the ip or i.n. routes of infection became diabetic. Mice were more resistant to the infectious and diabetogenic properties of the virus when given by the i.n. than when given by the ip route. Glycosuria in mice given virus i.n. usually lagged one day behind that in mice given virus …

The Immunological And Neurochemical Toxicity Of Benzene And Its Interaction With Toluene In Mice, Gin-Chang Hsieh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Benzene and toluene are known groundwater contaminants . Male CD-I mice were continuously exposed to 0, 31, 166, and 790 mg/ L benzene and 0, 17, 80, and 405 mg/L toluene, respectively, in drinking water for four weeks. Benzene caused a reduction of leukocytes, lymphocytes and erythrocytes, and resulted in a macrocytic anemia. Lymphocyte response to both B- and T-cell mitogens, mixed lymphocyte response to alloantigens, and the ability of cytotoxic lymphocytes to lyse tumor cells were enhanced at the lowest dose of benzene and depressed in the higher dosage animals. Benzene at doses of 166 and 790 mg/L decreased …

Effect Of Feeding A Viable Yeast Culture On Ruminal Fermentation Characteristics, Milk Production Response And Apparent Nutrient Digestibility In Holstein Cattle, Mohamed H. Shokair

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Four barren Holstein cows fitted with ruminal fistulas were assigned to each of two dietary treatments in a replicated 2 x 2 Latin square design. Treatments consisted of a basal ration and a basal ration plus 10g/d of a viable yeast culture (Saccharomyces cerevisiae). Cows were fed treatments for a 21-day adaption followed by a 7-day collection period. Total ruminal bacteria, cellulolytic bacteria and protozoa were unaffected by treatment. Feeding the viable yeast culture significantly increased ruminal acetic acid and acetic/propionic ratio. Molar percentage of propionic, isobutyric, isovaleric and valeric acids and rumen ammonia-N levels were significantly reduced …

The Modeling And Measurement Of Respiratory Carbon Use And Net Carbon Gain Of Two Agropyron Bunchgrasses, Halldor Thorgeirsson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The rate of photosynthetic carbon fixation and of root and shoot respiratory carbon use was measured in the laboratory and in the field (shoots only) for Agropyron desertorum (Fisch. ex Link) Schult. and Agropyron spicatum (Pursh) Scribn. and Smith. The rate of respiratory carbon use of the root system declined within hours of the shading or defoliation of the shoot system, resulting in as much as 60% reduction in specific rate of root respiration. The mean whole-plant growth efficiency (the ratio of whole-plant net carbon gain to gross photosynthetic carbon fixation) in full irradiance in the laboratory was 0.53 and …

Electromagnetic Field Variations In The Activation Of Bone Cell Synthesis, Gary Ketcherside

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Program Abstracts, 100th Session, Iowa Academy Of Science, April 21-23, 1988, Iowa Academy Of Science

Iowa Academy of Science Documents

Presentation abstracts from the annual meeting of the Iowa Academy of Science

The Gamut: A Journal Of Ideas And Information, No. 23, Spring 1988, Cleveland State University

The Gamut Archives

CONTENTS OF ISSUE NO. 23, SPRING, 1988

Louis T. Milic: Editorial, 2

Bearing Witness to the Best

Vincent Dowling: Interview: The Abbey Theatre-For Ireland and the World, 5

New artistic director charts ambitious course for "international Irish" drama.

Barbara Green: Machiavelli and the Problem of Evil, 17

Does Machiavellian have to mean evil?

Bruce A. Beatie: John Griswold White and His Libraries, 29

The Cleveland Public Library's unrivaled collections of chess lore, folklore, and Orientalia.

George A. Mauersberger: A Stage Set, a Bucket, a Cave, 43

Portfolio of drawings.

Samuel M. Savin: The Great …

Santa Clara Magazine, Volume 30 Number 3, Spring 1988, Santa Clara University

Santa Clara Magazine

10 - IS MARRIAGE REALLY INDISSOLUBLE? Santa Clara's noted marriage scholar examines the sacrament of marriage from an historical perspective. By Theodore J. Mackin, S.J.

14 - MENTOR AND GADFLY In a companion piece, marriage scholar Ted Mackin, S.J., is the subject of a profile. By Katherine Ann Tanelian and Paul Hennessy

17 - ON THE ROAD WITH JESSE JACKSON An AP reporter and alumna takes us behind the scenes to learn what it's like covering , a candidate.

21 - FEATS OF CLAY Clay Barbeau '59 is one of the hottest personalities on the counseling psychology circuit today. By …

Louisiana Agriculture Magazine, Spring 1988, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.