Digital Commons Network^™

Building Genetic And Culturing Tools For The Understudied Soil Bacterium E. Flagellatus, Aaron Butler, Brenda Pratte, Lon Chubiz

Undergraduate Research Symposium

Soil bacteria play essential roles in nutrient cycling, yet many abundant groups remain poorly characterized due to challenges in cultivation and genetic manipulation. One such organism is Edaphobacter flagellatus, a member of the Acidobacteriota frequently detected in soil environments but lacking well-developed experimental tools. To address this limitation, our laboratory has generated a genome-wide mutant library using the Tn5 transposon, enabling random disruption of genes across the population.

This project aims to establish culture conditions necessary to support the use of this library for functional genomic studies. In particular, defined minimal media can impose selective pressures that reveal …

Bacterial Biomass As A Versatile Platform For Functional Materials: Structural Insights And Sustainable Applications, Hasan M. Agha

AUIQ Complementary Biological System

Bacterial biomass has emerged as a versatile material with strong potential in environmental, material, and biotechnological applications, as well as broader uses such as bioplastics, packaging materials, and agricultural systems, owing to its rapid growth, controllable production, and surface chemistry enriched with carboxyl, hydroxyl, amino, phosphate, sulfate, and sulfhydryl groups. These features enable multiple interaction mechanisms, including electrostatic attraction, ion exchange, surface complexation, hydrogen bonding, and π –π interactions, supporting applications in pollutant removal, wastewater treatment, composite development, and bioenergy systems. Bacterial biomass can be sourced from natural environments, industrial fermentation, and waste streams, particularly activated sludge, making it compatible …

A Single-Step Plate Method For The Screening Of Xyl-A Mutant Libraries Based On Gene Expression, Bilqees Fatima, Muhammad Mohsin Javed

Journal of Bioresource Management

A single-step plate method is described for the screening of transformants of cloned gene xyl-A encoding xylose isomerase. It is rapid, inexpensive, easy to perform, and more specific, as the selection is based on xyl-A expression level. The crucial composition of the plate medium comprised agar, buffer, nutrients, co-factor, substrate, inducer, antibiotic, and an indicator, i.e., 2, 3, 5-triphenyl tetrazolium chloride (TTC). Transformed cells of E. coli BL21 (DE3) with a plasmid (pET21(a)⁺+xyl-A), were exclusively capable of growing, isomerizing the substrate, and producing deep-red formazan by reducing TTC, whereas the untransformed cells and cells containing a …

Faktor-Faktor Yang Berhubungan Dengan Kepatuhan Minum Obat Anti Tuberkulosis (Oat) Di Kota Samarinda, Fahmi Nur Indah Sari, Irfansyah Baharuddin Pakki, Nur Rohmah, Siswanto Siswanto, Muhamad Z. Saefurrohim

Jurnal Epidemiologi Kesehatan Indonesia

Latar Belakang: Tuberkulosis (TB) merupakan penyakit menular yang masih menjadi masalah kesehatan masyarakat utama di Indonesia. Keberhasilan pengobatan sangat bergantung pada kepatuhan pasien dalam mengonsumsi Obat Antituberkulosis (OAT) .

Tujuan . Untuk mengetahui hubungan antara persepsi kerentanan, keparahan, manfaat, hambatan (stigma), isyarat untuk bertindak (peran tenaga kesehatan), dan efikasi diri dengan kepatuhan minum OAT pada pasien TB di Kota Samarinda.

Metode . Penelitian ini menggunakan desain potong lintang dengan pendekatan kuantitatif yang melibatkan 121 pasien TB dari empat Puskesmas di Samarinda. Sampel dipilih menggunakan teknik multistage sampling. Data dikumpulkan melalui wawancara terstruktur menggunakan kuesioner dan dianalisis menggunakan uji Chi-Square.

Hasil …

Ecological And Public Health Consequences Of Sand Mining In Aquatic Ecosystems: A Systematic Review, Uduak Sylvanus Jacob, Isangedighi Asuquo Isangedighi, Olusola Abayomi Akangbe, Emmanuel Dami Omogbemi

Journal of Bioresource Management

The persistent exploitation of the aquatic ecosystem, driven by increasing demand for aquatic resources, including construction-grade sand, is evidenced by hydrological and geomorphological degradation of the aquatic ecosystem, with severe negative consequences on the fauna and flora communities. The rising global demand for sand and inefficient mining regulations have resulted in indiscriminate and illegal sand mining globally. However, developing an effective policy framework for sustained river sand mining requires local, regional, and global data (both qualitative and quantitative) on the status, drivers and implications of sand mining. This paper uses a systematic literature review approach to compile the ecological and …

Bifurcation Dynamics Of A Harvested Predator-Prey Model, Hala H. Emam, Mohamed F. Abouelenein, Mona Anis, M. Y. Hamada, F. A. Atia, H. El-Metwally

Mathematical Modelling and Numerical Simulation with Applications

In ecosystems, the harvesting effect is essential to preserving the relationships between prey and predator. This work examines a model of predator-prey in discrete time with harvesting. The Jacobian matrix's eigenvalues are calculated for the associated fixed points. We examine the dynamical and qualitative analysis of this model, look into the criteria for stability and bifurcation, and analyze analytical results that show the rich dynamics and complexity of this model. We give sufficient circumstances for the Period-doubling at the interior equilibrium point by employing the harvesting effect. The discrete system's chaos control is carried out, and the outcomes are supported …

The Use Of Gadgets And Learning Motivation Among Grade Xi Students In Biology Learning At Sma Negeri 5 Palu, Yulia Citra, Mohammad Jamhari, Masrianih Masrianih, Lestari M.P Alibasyah, Mursito S. Bialangi, Yulia Windarsih

Jurnal Pendidikan Sains

This study aims to describe the profile of gadget use and learning motivation among Grade XI students in biology learning at SMA Negeri 5 Palu and to explore students’ and teachers’ experiences of gadgets as learning support tools and potential sources of digital distraction. A qualitative descriptive design was employed, supported by descriptive questionnaire profiling. A total of 74 students completed a 50-item questionnaire on gadget use and learning motivation, while 10 students and two biology teachers participated in semi-structured interviews. Questionnaire data were analyzed using frequencies and percentages, while interview data were analyzed thematically. The descriptive findings showed that …

The Application Of The Group Investigation (Gi) Model Of Cooperative Learning To Improve Students’ Understanding Of Biodiversity In Year 10 At Sman 2 Mori Atas, Nadia Rosmita Lumako, Mohammad Jamhari, Yulia Windarsih, Lilies Lilies, Rafiqa Rafiqa, Amalia Buntu

Jurnal Pendidikan Sains

Biology instruction at SMAN 2 Mori Atas was predominantly conducted through conventional teaching practices. Preliminary observations revealed that students had difficulty understanding biodiversity concepts, and several students did not meet the minimum passing criterion of 70. These conditions highlighted the need for learning activities that promote active investigation, discussion, and conceptual connections. This classroom action research examined the use of the Group Investigation (GI) cooperative learning model to improve Grade 10 students’ classroom engagement and conceptual understanding of biodiversity, as assessed through Higher-Order Thinking Skills (HOTS) questions based on the Structure of Observed Learning Outcomes (SOLO) taxonomy. The study involved …

The Cognitive Construction Of Sustainable Environmental Awareness Through Green Language Pedagogy In Second Language Acquisition, Meral Yavuz Kartal, Osman Yılmaz Kartal

Journal of Environmental Science and Sustainable Development

The escalating global ecological crisis highlights the need for new approaches to sustainability education, while the cognitive role of Second Language Acquisition (SLA) remains underexplored. This study employs a critical literature review with a systematic search and qualitative thematic synthesis to develop a proposed Green Language Pedagogy (GLP) model. The review followed a four-stage screening protocol (identification, screening, eligibility, and inclusion) and analyzed 50 theoretical and empirical sources published between 2014 and 2025. This analysis of the selected literature reveals a stark disparity: an estimated 85% of the reviewed environmental education research focuses on factual knowledge transfer, whereas only 15% …

Poisonous And Injurious Vascular Plants Of The United States: An Introduction, James P. Smith Jr

Botanical Studies

No abstract provided.

Hbb Gene Mutations And Their Impact On Phenotype And Protein Function In Β-Thalassemia Families From Dera Ismail Khan, Pakistan, Muhammad Ayaz, Muhammad Muzammal, Maria Shafiq, Nisar Ahmad, Dua Iman, Maria Faraz, Yaser Uddin Hoti, Sami Siraj, Muzammil Ahmad Khan, Jabbar Khan

Makara Journal of Science

The current genetic study aimed to screen out the genetic basis of ß-thalassemia in five unrelated consanguineous Pakistani families. Blood samples were collected, and targeted Sanger Sequencing of the HBB gene was done. Serum Interleukin-6 (IL-6) and quantitative measurements of T Helper and T Suppressor Lymphocytes were also carried out. Additionally, in silico analysis, including protein modeling, Protein-Protein docking, and Multiple sequence alignment, was also performed. Genetic analysis identified a homozygous mutation c.92+5G > C (IVS-I+5 G > C) in families 1, 2, and 3, which resulted in β⁺/ β⁺ Thalassemia Intermedia phenotype. While in the case of family …

Combined Antifungal Activity Of Secondary Metabolites From Piper Aduncum And Additive Oils Against Sclerotium Rolfsii, A Causal Agent Of Stem Rot On Patchouli Cuttings, Herwita Idris, Nurmansyah Nurmansyah, Syahrial Abdullah, Riska Riska, Tri Budiyanti, Afrizon Afrizon, Jonharnas Jonharnas, Siti Rosmanah, Taufik Hidayat, Helfi Gustia

Makara Journal of Science

This study aimed to evaluate the antifungal activity of Piper aduncum secondary metabolites along with additive oils against Sclerotium rolfsii, causing stem rot on patchouli cuttings. It was conducted in two stages—an in vitro experiment using a completely randomized design with seven treatments: P. aduncum oil, combinations of P. aduncum oil with citronella grass, lemongrass, cinnamon leaf, and cloves leaf oils, a synthetic pesticide (propineb 70%), and without essential oils. The second was carried out in vivo using a factorial completely randomized design, in which the first factor was five antifungal formulations, and the second was the spraying interval. …

Changes In Seasonal Use Of The Central Platte River Valley, Nebraska, By Sandhill Cranes, Bethany L. Ostrom

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Recent environmental change has altered migration timing and winter distribution of many bird species, including Sandhill Cranes. Historically, the Central Platte River Valley in Nebraska functioned primarily as a spring staging area for the midcontinent population, with limited fall use and rare overwintering events. I examined records of Sandhill Crane occurrences during October to January in the Central Platte River Valley from 2015-2026 using incidental observations and aerial surveys starting in 2024. Overwintering Sandhill Cranes were documented in 7 of 11 Dec/Jan, with unprecedented increases observed since 2023, peaking with approximately 42,000 Sandhill Cranes in January of 2026, the largest …

A List Of The Vascular Plants Of Iowa, James P. Smith Jr

Botanical Studies

No abstract provided.

Differences In Mental Toughness Among Mixed Martial Artists And Physically Active Individuals, Savannah M. Calleja, Soleil Ramos, Victoria Potvin, Anthony A. Ricci, Gabriel J. Sanders, Corey A. Peacock

Journal for Sports Neuroscience

Mixed Martial Arts (MMA) is a physiological and psychological demanding sport. While the majority of the literature conducted in MMA regards physiological health and risk of injury, researchers are beginning to assess the psychological profiles of MMA athletes. Previous studies have evaluated mental toughness (MT) within MMA; however, no study has examined MT in MMA athletes at the professional division. Therefore, the primary purpose of this research was to evaluate the difference in mental toughness between professional MMA athletes and physically active individuals. Secondary aims are to evaluate potential variations in mental toughness within the professional level between champion and …

Legal Shifts And Judicial Inconsistencies In Strict Liability After The Omnibus Law: A Critical Analysis Of Indonesian Environmental Law For Responsible Mining And Esg Compliance, Nurul Listiyani, Khenza Atthaya Namira Yulianto

Journal of Environmental Science and Sustainable Development

The legal significance of strict liability in mining arises from the sector’s capacity to generate technically complex, diffuse, and long-latency environmental harm that is difficult to address through fault-based litigation alone. This study aims to evaluate how the post-Omnibus Law reformulation of Article 88 of Law No. 32 of 2009 has affected the doctrinal autonomy, judicial consistency, and practical enforcement of strict liability in Indonesian environmental law. The research used normative doctrinal legal research including statutory, case-law, conceptual, comparative, and evaluative analyses. The data cover the 2009–2025 period and consist of two core statutory formulations of Article 88, the Job …

Phosphoric Acid-Mediated Regeneration Of Sugarcane Bagasse Cellulose-Rich Fraction For Enzymatic Production Of Water-Soluble Cellulosic Oligomers, Chiou-Yeong Saw, David Agus Setiawan Wibisono, Tsung-Yen Wu, Yung Chang, Chi-Fai Chau

Journal of Food and Drug Analysis

An integrated strategy was developed to promote conversion of a sugarcane bagasse cellulose-rich fraction into water-soluble cellulosic oligomers (WCOs) through phosphoric acid-mediated regeneration and enzymatic hydrolysis. Alkaline pretreatment on sugarcane bagasse powder increased glucan content from 57.6 to 93.4 g/100 g sample and lowered Klason lignin to 6.1 g/100 g sample. Phosphoric acid-mediated regeneration was subsequently capable of reorganizing the structure of cellulose from sugarcane bagasse, resulting in a decrease of crystallinity from 72.9 to 64.0% and introducing cellulose II-like features. The hydration state of regenerated cellulose strongly influenced hydrolysis performance, as the hydrated form showed significantly higher solubilization (810.4 …

Study On Preparation And Emulsification Properties Of Distillers Dried Grains With Solubles Arabinoxylan Hydrolysate/Soy Protein Isolate Complex, Yu Shuo, Guo Qingbin, Zhao Shudi, Li Zhenjing, Jia Xing, Kang Ji

Food and Machinery

[Objective] To expand the application fields of distillers dried grains with solubles and improve its market value. [Methods] Arabinoxylan (AX) was extracted from distillers dried grains with solubles and modified to prepare AX with different molecular structures. AX was then reacted with soy protein isolate (SPI) via the Maillard reaction to prepare the AX-SPI covalent complex. The structure of the complex was characterized using circular dichroism spectroscopy, Fourier transform infrared spectroscopy, particle size, and zeta potential, and its emulsifying properties were studied. [Results] The AX-SPI covalent complex is formed by SPI and AX via the Maillard reaction, and differences in …

Effect Of Sodium Butyrate On Biofilm Formation And Virulence Of Salmonella Typhimurium, Yao Meng, Li Zhiheng, Wang Yan, Pan Wanlong, Liu Peng

Food and Machinery

[Objective] To investigate the regulatory effects of sodium butyrate on biofilm formation by Salmonella typhimurium and clarify its effects on the expression of virulence-related genes and pathogenicity under in vivo conditions. [Methods] S. typhimurium was treated with 4 mg/mL and 8 mg/mL sodium butyrate, and the effects of sodium butyrate on biofilm formation, motility, metabolic activity, and quorum sensing of the strain were detected. The expression changes of virulence genes (hilA, invF, and sipA) in sodium butyrate-treated strains were analyzed by quantitative real-time PCR. A C57BL/6 mouse infection model was established using S. typhimurium pretreated with 8 mg/mL sodium butyrate, …

Overexpression Of Liax Enhances Stress Tolerance In Lacticaseibacillus Paracasei L9, Liu Ting, Ma Xiayin, Yan Ningning, Chen Shihao, Bai Jingfu

Food and Machinery

[Objective] To investigate the protective role of the surface protein LiaX in Lacticaseibacillus paracasei L9 against external stresses, such as bile salts, antibiotics, and the gastrointestinal environment. [Methods] A LiaX-overexpressing strain was constructed and the protein was purified. Spent media assays, in vitro simulated digestion, and exogenous protein supplementation experiments were comprehensively employed to evaluate the effect of LiaX on the survival rate of the strain under digestive, bile salt, and antibiotic stresses. [Results] The LiaX-overexpressing strain LPL9psip0968 his was successfully constructed, and the LiaX protein was isolated and purified. Spent media assays and exogenous addition of purified LiaX protein …

Preparation , Structural Characterization , And In Vitro Hypoglycemic Activities Of Acetylated Elaeagnus Moorcroftii Wall . Polysaccharides, Xue Xue, Tang Hui, Liao Bingwu, Chen Lu, Zhao Wenbin, Wang Xinbing, Sun Yan, Wei Youhong

Food and Machinery

[Objective] To explore the preparation conditions, structural information, and in vitro hypoglycemic activities of acetylated Elaeagnus moorcroftii Wall. polysaccharides of Kashgar (A-EMP-K). [Methods] EMP-K is extracted by the water extraction and alcohol precipitation method, and modified by the acetic anhydride method. Then, A-EMP-K preparation conditions are optimized using Box–Behnken response surface methodology. Furthermore, structural characterization and in vitro hypoglycemic activity assays of A-EMP-K are performed. [Results] The optimal preparation conditions are a reaction time of 3 h, a reaction temperature of 61 ℃, and an acetic anhydride addition volume of 9.3 mL. Under these conditions, A-EMP-K exhibits a degree of …

Comparison Of Preenrichment And Screening Methods For The Detection Of Salmonella Enterica Serovar Agona In Meat Analog Products, Kylie Sacapano, Amanda Tabb, Biola Fatusin, Samantha M. Covaia, Isha Shingre, Lieuchi Phan, Kyson Chou, Jamie Du, Beilei Ge, Donna Williams-Hill, Rosalee S. Hellberg

Food Science Faculty Articles and Research

With the growing demand and variety of meat analogs on the commercial market, there is a need to revisit testing methods for foodborne pathogens in these products. The U.S. Food and Drug Administration (FDA) regulatory methods for Salmonella enterica detection in meat analogs follow the Bacteriological Analytical Manual and are relatively time-consuming. Therefore, this study aimed to compare preenrichment and screening methods for the detection of Salmonella in meat analogs by evaluating current FDA reference methods alongside alternative preenrichment broths and rapid molecular screening techniques. Meat analog samples were inoculated with S. enterica serovar Agona at three levels: (1) uninoculated …

Barriers And Facilitators To Health Literacy Among Black Americans With Overweight And Obesity In South Carolina: A Mini-Ethnographic Study, Shelly Campbell-Timmons

Doctoral Dissertations and Projects

The purpose of this qualitative mini ethnography adapted from traditional ethnography with limited time and scope, was to explore and understand perceptions of barriers and facilitators to health literacy among Black Americans with overweight and obesity in three South Carolina counties. Population data showed that approximately 40.3% of U.S. adults are obese. Moreover, Non-Hispanic Black communities are disproportionately affected by overweight and obesity despite efforts to combat the weight-related health risk. Still, limited health literacy should be the focus of public health strategies like teach-back and plain language communication, to address overweight and obesity prevalence and disparities. The study was …

Revealing Cryptic Lineage Diversity In The Persian Gulf And Oman Sea Groupers (Perciformes: Epinephelidae): Phylogenetic And Species Delimitation Insights, Ehsan Damadi, Faezeh Yazdani Moghaddam, Masoumeh Gholipour Cheshm Gilak, Mehdi Ghanbarifardi

Turkish Journal of Zoology

Groupers (Epinephelidae) are a taxon of great commercial interest due to their large body size and high market value, and are also an important ecological species for biodiversity conservation in coral reef ecosystems. Despite their importance, phenotypic convergence among species generally leads to incorrect taxonomic identification, an issue enhanced by molecular studies revealing the presence of a high cryptic diversity and complicating conventional systematic frameworks. Herein, grouper species diversity in the ecologically heterogeneous Persian Gulf and Oman Sea was investigated to resolve taxonomic ambiguities and assess patterns of cryptic diversity. Mitochondrial COI gene sequencing was used in conjunction with integrative …

Otolith Morphological Variations Reveal Intraspecific Diversity In Two-Spot Barb, Pethia Ticto (Hamilton, 1822), Inhabiting The Riverine Ecosystem, Insha Hassan, Lubna Yasmeen, Mohd Sadiq, Ameer Hamza, Mohammad Afzal Khan

Turkish Journal of Zoology

The intraspecific variation in otolith wavelet coefficients, contours, and morphometric variables (six size and nine shape indices) was analyzed to discriminate stock variation in Pethia ticto collected from four selected sites along the River Ganga (Narora, Kanpur, Varanasi, and Bhagalpur). The wavelet coefficients extracted revealed symmetrical yet site-specific contour variations, particularly along the rostral, dorsal, and posterior margins, with the fourth-level wavelet explaining 98% of the cumulative accuracy and being selected for contour analysis. Permutational analysis of multivariate dispersion (PERMDISP) (F = 6.11, p = 0.002) and permutational multivariate analysis of variance (PERMANOVA) (F = 8.42, R2 = 0.179, p …

Toxicokinetics And Multibiomarker Assessment Of Coexposure To Sulfamethoxazole And  Titanium Dioxide Nanoparticles In Cyprinus Carpio, Nazish Iftikhar, Rabeea Zafar, Imran Hashmi, Asma Jamil, Christopher Joseph Martyniuk, Munib Ahmed Shafique

Turkish Journal of Zoology

The combined effects of pharmaceuticals and nanoparticles (NPs) on aquatic organisms remain insufficiently characterized. The present study examined toxicokinetics and multibiomarker responses of Cyprinus carpio (common carp) following exposure to sulfamethoxazole (SMX) and titanium dioxide (TiO₂) NPs. Juvenile fish were treated with SMX (25, 50, and 100 μg/L) alone and in combination with 1.5 mg/L TiO₂ NPs (96 h). TiO₂ NPs had a significant effect on the bioaccumulation of SMX in muscle tissues, with SMX reaching peak concentrations of 2286.54 ng/g in the 100 μg/L SMX + 1.5 mg/L TiO₂ NPs group after 72 h, compared to 1477.2 ng/g with …

Uae Audiences' Reliance On Social Media As A Source Of Information On Complementary And Alternative Medicine: A Study Of Al Ain City Residents Based On Media System Dependency Theory, Abdul Rahman Al-Toum, Mohammed Al-Kaabi, Najoud Al-Mansouri, Ahmed Bin Ishaq, Amer Khaled Ahmad, Maram Manajrah

Middle East Journal of Communication Studies

This study examined the extent to which UAE audiences rely on social media as a source of information on complementary and alternative medicine (CAM) and the resulting effects of this reliance. Grounded in Media System Dependency Theory, the study employed an audience survey using a questionnaire administered to a sample of (132) Emirati citizens in Al Ain. The findings revealed that the combined medium and high reliance reached (53%). Regarding the effects, cognitive effects recorded the highest arithmetic means, followed by affective and behavioral effects at comparable levels. Hypothesis testing showed no statistically significant differences in the degree of reliance …

Reliable Performance Analysis Of Polyurea Coatings Used In Offshore Wind Turbines, Yu-Chang Lin, Yen-Fu Liu, I-Ming Pan, Ming-Yuan Lin, Kang-Yu Liao, Hung-Hua Sheu, Hung-Bin Lee

Journal of Marine Science and Technology–Taiwan

This study explores the weather resistance, mechanical properties and corrosion resistance of polyurea coatings in marine environments. The relevant analysis results can be used as a reliability assessment of offshore wind turbine structural coatings during their service life under the influence of marine environments. The long-term experiment uses seawater and 70°C thermal aging conditions as coating reliability assessment items. The microstructure of the samples that have been immersed in seawater for a long time and thermally aged is observed using a scanning electron microscope (SEM). The results show that the surface of the 100 and 125 series polyurea coatings is …

Ddrr Acts As A Dna Damage-Induced Cell Division Inhibitor In Acinetobacter Baumannii, Christian P. Lowe, Deborah Cook, Lily Hoard, Mckinnley Martin, Janelle M. Hare

Acinetobacter baumannii DNA Damage Database

An article titled, "DdrR acts as a DNA damage-induced cell division inhibitor in Acinetobacter baumannii" published in the Journal of Bacteriology on September 9, 2026.

Amorphous Calcium Phosphate Maintains Casein Microarchitecture While Modulating Bacterial Growth Kinetics, Vuk Uskoković, Michael Ibba, Lorenzo Eugenio Leiva

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Casein, the primary source of protein in ruminant milk, represents a family of proteins that self-assemble into globules composed of finer spherical subunits and stabilized by calcium phosphate (CP) nanoparticles. These globules act as holdase-type chaperones for unfolded or partially folded proteins, preventing their aggregation. Simultaneously, by integrating CP nanoparticles, they enable the bioavailability of calcium and phosphate beyond their solubility limits, thereby enhancing the nutritional value of milk. Using a range of physicochemical and biological characterization techniques, including scanning and transmission electron microscopy, organic and inorganic elemental analyses, SDS-PAGE, and vibrational, UV/Vis and NMR spectroscopies, it is demonstrated that …