Digital Commons Network^™

Development Of A Multi-Institutional Dataset To Validate A Novel Inflammatory Breast Cancer Diagnostic Score, Filipa Lynce, Samuel M Niman, Megumi Kai, Sean Ryan, Elizabeth Troll, Li Li, Kathy D Miller, Reshma Jagsi, Ginny Mason, Beth A Overmoyer, H T Carisa Le-Petross, Faina Nakhlis, Savitri Krishnamurthy, Beth T Harrison, Susie X Sun, Eren D Yeh, Jennifer R Bellon, Laura E Warren, Michael C Stauder, Meredith M Regan, Wendy A Woodward

Faculty, Staff and Student Publications

Background: Susan G. Komen, the Inflammatory Breast Cancer (IBC) Research Foundation, and the Milburn Foundation convened patient advocates, clinicians, and researchers to propose novel quantitative scoring rubrics for IBC diagnosis. In this study, we developed a multi-institutional clinical dataset to test and validate the proposed scoring system.

Methods: IBC (N = 988) and non-IBC (N = 332) cases were identified at 2 institutions with dedicated multidisciplinary IBC programs. The non-IBC cohort included consecutive cT4b and cT4c patients. Standard operating procedures (SOPs) were developed for all ambiguous findings and languages. Three different methods were used for the imputation of missing data, …

What’S The Beef With Agriculture? Evaluating The Effectiveness Of An Agricultural Literacy Intervention On Students’ Attitudes And Knowledge Toward Sustainability And Animal Agriculture, Jenny E. Bennett

All Graduate Theses and Dissertations, Fall 2023 to Present

Agricultural literacy is essential for helping individuals understand how food systems impact their daily lives and society. While most research has focused on students’ knowledge, this study addresses the underexplored role of attitudes—critical predictors of behavior—as outcomes of educational interventions. This research analyzed survey data collected before and after an agricultural literacy intervention delivered to high school students in school-based agricultural education (SBAE) programs. The curriculum focused on sustainability in animal agriculture.

Findings from paired samples t-tests showed statistically significant improvements in students' attitudes (from moderately neutral to more positive) and knowledge scores. Further analyses revealed that demographic factors such …

Electrophysiological Markers Of Emotion Dysregulation In Autistic Youth, Rachel G. Mcdonald

Theses, Dissertations and Culminating Projects

Emotion dysregulation (ED) is a transdiagnostic mechanism that is conceptualized as difficultly regulating emotions and deploying appropriate regulation strategies in response to environmental stimuli. Meta-analyses suggest that autistic people, across the lifespan, experience more severe ED as compared to both neurotypical people and those with mental health conditions. Despite elevated risk and evidence linking ED to disruptions in neural mechanisms, no studies to date have examined neural markers of ED in autistic populations. The current project uses multi-method and multi-informant measurement to examine similarities and differences in neural mechanisms of ED in autistic and non-autistic populations. Forty-four youth aged 11—17 …

Isolation And Characterization Of Siderophore(S) From Pseudomonas Mosselii, Casey J. Stophel

Electronic Theses and Dissertations

Iron is essential for growth of most microorganisms. It functions as an important cofactor in many important biological processes. However, iron found in soil is rendered insoluble and unavailable to microorganisms for use. To overcome this, many bacterial species produce siderophores, a small organic molecule that has high affinity for iron. Siderophores allow for bacterial cells to obtain otherwise insoluble iron. Pseudomonas mosselii, a Gram negative bacterium, was isolated from a local soil sample. P. mosselii was found to produce siderophore(s) under iron restricted environment, however, they have not been characterized. Therefore, it was of interest to isolate, purify and …

Host-Virus Interaction Dynamics Using Sulfolobus Spindle-Shaped Virus And Host Sulfolobales System, Yeasin Ahmed

Graduate Theses and Dissertations

The Sulfolobus Spindle-shaped virus (SSV) is among the most studied archaeal viruses, particularly in extreme environments. This virus predominantly infects multiple Crenarchaea from the Sulfolobaceae family. These hosts thrive in extreme environmental conditions, such as sulfuric hot springs, with a pH of 2 to 4 and a temperature of 76-80˚C. Sulfolobales exhibit a wide geographical distribution across volcanic hot springs. The Sulfolobus Spindle-shaped Virus (SSV), encompassing strains such as SSV1 and SSV8, employs a non-lytic replication strategy in hosts belonging to the order Sulfolobales. The SSV-Sulfolobales system can be cultured in liquid and gellan gum solid media. A comprehensive study …

The Influence Of Retail Display Conditions On Ground Beef Quality And Palatability, Sarah Lynn Shoup

Graduate Theses and Dissertations

The objectives of these studies were to evaluate the impact of retail display case type and muscle type on meat quality attributes, including color, lipid oxidation, texture, and volatile compound profiles, as well as to assess economic implications based on retail color degradation. Case type effects were further evaluated by considering environmental factors such as temperature, air velocity, and relative humidity and their influence on the shelf life of beef patties. An economic analysis was also conducted to estimate the value of patties based on a* (redness) scores at different points during retail display. In Study 1, differences in L* …

The Bacteria Who Lived: Uncovering And Mitigating The Persistence Of Pathogens In Low-Moisture Food Processing, Kavita Shripati Patil

Graduate Theses and Dissertations

Low-moisture food (LMF) processing environments pose a persistent food safety risk due to Salmonella survival and harborage on surfaces. This work investigates persistent bacterial populations (PBPs) in LMF production environments, focusing on identifying a suitable surrogate and evaluating sanitation strategies. Three potential surrogates, Escherichia coli MP 26, Enterococcus faecium, and Pediococcus acidilactici, were compared to Salmonella PBPs formed in nonfat dry milk (NFDM) on stainless steel coupons stored at 25°C under 30% or 70% relative humidity (RH) using two different inoculation methods. E. faecium showed survival patterns most similar to Salmonella, with no significant differences in log reductions (p > 0.05), …

Effects Of Flavonoids On Reducing Colon Cancer Risk Via Macrophage Polarization, Gracien Jules

Graduate Theses and Dissertations

Colorectal cancer is one of the most fatal diseases in the United States. Chronic tissue inflammation can cause cell proliferation, which leads to tumor formation and cancer development. Macrophages are responsible for maintaining tissue homeostasis and for inflammatory responses. M1 macrophages exhibit proinflammatory responses while M2 macrophages exhibit anti-inflammatory responses. Imbalance between M1 and M2 macrophages is closely related to inflammation-associated disease development. The anti-inflammatory effects of apiaceous vegetable constituents, specifically flavonoids, have been studied for their preventative effects towards reducing disease risk. However, sufficient evidence is lacking on the mechanisms by which flavonoids impact M1/M2 macrophage polarization. RAW 264.7 …

Ecosystem Services Of Urban Green Spaces In Louisville, Ky., Julia Kachanova

Electronic Theses and Dissertations

Accelerating urbanization poses critical challenges to urban green spaces (UGS), vital for ecological balance and human health. Rapid development often results in fragmented habitats, biodiversity loss, and diminished ecosystem service delivery. There is an urgent need to explore and evaluate novel strategies capable of enhancing urban resilience, such as implementing urban orchards, and managing existing parks and gardens in sustainable ways. This dissertation explores how different forms of urban green spaces (UGS) contribute to the provision and maintenance of critical ecosystem services that support human well-being and urban biodiversity. By analyzing UGS impact on ecosystem service provisioning and long-term sustainability, …

The Influence Of Psychological, Biological, And Social Determinants On Disordered Eating Behaviors Among U.S. Young Adults: Evidence From Nhanes 2015-2018., Samantha Kaye Gerbine

Electronic Theses and Dissertations

Disordered eating behaviors (DEBs) are influenced by biological, social, and psychological factors, yet research often narrowly focuses on White, college-aged women. This study assessed the predictors and prevalence of DEBs in a nationally representative sample of U.S. young adults using data from the 2015–2018 National Health and Nutrition Examination Survey (NHANES). Survey weights were applied to generate national estimates. Descriptive statistics characterized the sample, and logistic regressions tested biological, social, and psychological predictors of DEBs. A composite risk score was developed using variables retained in the final model. The unweighted sample (n = 1,062) represented 64 million U.S. young adults. …

Acquired Resistance In Cancer: Towards Targeted Therapeutic Strategies, Alice Soragni, Erik S Knudsen, Thomas N O'Connor, Cristina E Tognon, Jeffrey W Tyner, Beatrice Gini, Donghwa Kim, Trever G Bivona, Xingxing Zang, Agnieszka K Witkiewicz, David W Goodrich, Dadi Jiang, Seth T Gammon, Christopher D Willey, Paul C Boutros, Vlad C Sandulache, Abdullah A Osman, Jeffrey N Myers, Kamiya Mehla, Pankaj K Singh, Keith S Chan, Hongbo Gao, Himangi Marathe

Faculty, Staff and Student Publications

Development of acquired therapeutic resistance limits the efficacy of cancer treatments and accounts for therapeutic failure in most patients. How resistance arises, varies across cancer types and differs depending on therapeutic modalities is incompletely understood. Novel strategies that address and overcome the various and complex resistance mechanisms necessitate a deep understanding of the underlying dynamics. We are at a crucial time when innovative technologies applied to patient-relevant tumour models have the potential to bridge the gap between fundamental research into mechanisms and timing of acquired resistance and clinical applications that translate these findings into actionable strategies to extend therapy efficacy. …

Characterizing Host And Viral Factors Relevant To Turkey Hemorrhagic Enteritis Virus-Induced Immunosuppression, Abraham Quaye

Theses and Dissertations

Turkey hemorrhagic enteritis virus (THEV) is an adenovirus (AdV) that causes hemorrhagic enteritis (HE) in turkeys, a debilitating disease affecting predominantly 6-12-week-old turkey poults characterized by immunosuppression (IMS) and intestinal lesions leading to bloody diarrhea. Although a naturally-occurring avirulent THEV strain has been adopted as live vaccine and has proven effective at preventing HE in turkeys, it still retains its immunosuppressive ability. Thus, vaccinated birds are rendered more susceptible to opportunistic infections and death than unvaccinated cohorts, leading to substantial economic losses. This dissertation focuses on elucidating both host and viral factors mediating THEV-induced IMS in turkeys. As THEV gene …

Characterizing Gastric Cancer Tumor Immune Microenvironment Through Extracellular Vesicles, Claire Elizabeth Bruno

Theses and Dissertations

The tumor immune microenvironment (TIME) critically influences cancer progression and determining therapeutic response. As immunotherapies continue to improve and reshape cancer treatments, there is a need for tools that can more efficiently and accurately assess and monitor the TIME. Extracellular vesicles (EVs), especially small EVs (sEVs), are lipid-bound nanoparticles that carry proteins, DNA, and RNA reflective of their cell of origin, offering a novel, non-invasive biomarker of the TIME. This makes them a promising candidate for immune profiling and treatment monitoring in cancer. This thesis investigates the potential of EVs as biomarkers for TIME composition and immune activity. An analysis …

Assessment Of Covid-19 Risks Related To Behaviour And Body Resistance In The Remote Area: Case Study In Jeneponto, Basri Basri, Andi Tilka Muftiah Ridjal, Lukman Setiawan, Renaldi M

Journal of Environmental Science and Sustainable Development

COVID-19 has posed significant global health threats, with responses varying across populations due to differences in risk perception, behaviour, and biological vulnerability. In Indonesia, most research has focused on urban settings, with limited attention paid to rural or remote communities. This study assesses COVID-19- risks related—specifically behavioural and immunity-related-factors—in the remote area of both Bontoramba Subdistrict, Jeneponto Regency. A cross-sectional study was conducted using cluster random sampling across eight villages, with 183 participants selected from a total of 12,242 households. Data were collected between November to December 2020 using a validated structured questionnaire, administered both online and face-to-face interviews. The …

Coal Mining Reclamation: Lessons Learned From Tropical And Subtropical Countries, Priyaji Agung Pambudi, Suyud Warno Utomo, Roni Setyo Handoko

Journal of Environmental Science and Sustainable Development

This study addresses the persistent environmental challenges associated with coal mining in tropical and subtropical regions, where complex microclimates and ecological variability hinder effective reclamation. Despite the increasing global coal demand, comparative cross-country insights remain limited. This research fills the gap by evaluating the best practices. This study employs a qualitative methodology with a comparative case study design, uses systematic literature review to evaluate ecology based reclamation policies and implementation practices. Data were sourced from peer-reviewed journals from Scopus and the Web of Science database and institutional reports, and analyzed using McHarg’s landscape ecology theory to evaluate reclamation policies, ecological …

Assessing The Transformation Of Livelihoods In Illegal Gold Mining Communities, Nurmansyah Ardikoesoema, Mahawan Karuniasa

Journal of Environmental Science and Sustainable Development

Illegal Artisanal Small-Scale Gold Mining (PETI) in Poboya, Palu City, exemplifies Indonesia’s nexus of socio-environmental vulnerability and institutional marginalization. Although environmental degradation, health impacts, and regulatory challenges associated with PETI have been extensively documented, limited research has systematically examined how the interplay of capital assets and internal motivation drives livelihood transformation pathways within PETI communities. To address this gap, this study uniquely integrates the Sustainable Livelihood Framework with spatial analysis to assess the combined influence of environmental, social, and economic capitals on PETI livelihood transitions. Using a mixed-methods approach, data were collected through quantitative survey and qualitative in-depth interviews with …

Environmental Impacts Of Land Use Change In Industrial Areas, Dwi Nowo Martono, Aruminingsih Aruminingsih

Journal of Environmental Science and Sustainable Development

Industrial development in Indonesia has been predominantly concentrated on Java Island, particularly in Karawang Regency, West Java Province. Covering an area of 175,327 hectares, Karawang has been identified as a strategic region for national food security. The Minister of Agrarian Affairs and Spatial Planning designated 95,667.45 hectares of rice fields as protected agricultural land. However, the expansion of industrial and residential areas within the regency poses a significant threat to its role as the national rice granary and to broader food security. As industrial land occupancy increases, the environmental capacity to support rice production and ensure national food security declines. …

Sustainable Water Management In Universities: Perceptions, Behaviours, And Challenges, Hastin Setiani, Dwita Sutjiningsih, Riri Fitri Sari

Journal of Environmental Science and Sustainable Development

Universities are increasingly adopting the “living laboratory” model to implement and evaluate sustainability innovations. These settings enable students, staff, and stakeholders to co-create and test real-world solutions, including those related to water use. Universitas Indonesia promotes sustainability through an integrated approach encompassing policy development, academics programs, and infrastructures. Prior studies using the Theory of Planned Behavior (TPB) have demonstrated that attitudes and perceived norms significantly influence sustainable behavior. However, limited research has examined how these dynamics operate within Indonesian universities or how university-based initiatives may contribute to broader urban water management. This study addresses that gap by examining perceptions, behaviors, …

Evaluation Of Sustainability Assessment Post Changes In The Management Of The Natural Reserve To The Natural Tourism Park In Kamojang Area, West Java, Indonesia, Purna Hindayani, Alnidi Safarach Bratanegara, Intan Adhi Perdana Putri, Zuzy Anna, Armandha Redo Pratama

Journal of Environmental Science and Sustainable Development

The area of Kamojang Nature Tourism Park, which was originally only 255 hectares, has expanded to 2,391 hectares following a changes in the Nature Reserve area. This change has raised concerns and objections from various parties, as it is considered to open opportunities for exploitation, threaten biodiversity, and potentially trigger tourism activities that may degrade the environmental quality of the areaThis study aims to analyze the sustainability status index of the Kamojang Natural Tourism Park following the status downgrade from a Nature Reserve to a Natural Tourism Park. A descriptive qualitative approach used, utilizing the Multi-dimensional Scaling model to determine …

Understanding Offline Pro-Environmental Identity: Factors And Implications, Tusyanah Tusyanah, Rahayu Puji Haryanti, Wijang Sakitri, Ismiyati Ismiyati, Nur Chayati

Journal of Environmental Science and Sustainable Development

Given global environmental challenges, developing sustainable behavior among pre-service teachers is crucial, as they will be responsible to educate future generations who must contribute to solving environmental problems. Offline pro-environmental identity refers as the individual's environmental commitment in real-life setting. Previous studies have primarily focused on online identity and behavioral intentions, while real-life behavioral context have not been fully examined. The goal of this study was to investigate the effects of the online pro-environmental identity, the perceived behavioral control, and the moral identity on the behavioral intention and the offline pro-environmental behavior. The study employed a quantitative research design and …

Improved Efficiency Of Plant Regeneration From Smooth Bromegrass (Bromus Inermis) Suspension Culture, T H. Chen, J Marowitch

IGC Proceedings (1977-2023)

Smooth bromegrass, Bromus inermis Leyss, is the most important forage grass in Canada. Breeding for improvements such as higher yield and faster regrowth has made little progress, mainly due to the polyploidy and cross-pollination which are characteristic of this grass. The application of tissue culture methods to bromegrass improvement holds promise only if an efficient procedure for plant regeneration is found. Various basal culture media and auxins were tested for their effects on callus induction, growth of suspension cultures and plant regeneration. None of the tested auxins gave better in vitro growth than 2,4-D. Eriksson's medium with 2,4-D as the …

Cultivating The Wild: Strategies For Entrepreneurs Navigating Niche And Specialty Food Markets, Trey Malone, Courtney F. Cooper

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Introduction Consumer demand for authenticity and sustainability in food systems is growing, creating both opportunities and challenges for entrepreneurs in niche and specialty markets. While wild-foraged and hunted foods are often perceived as more "natural" and sustainable, attempts to scale these products through farming may compromise their perceived authenticity.Methods To explore these dynamics, we conducted discrete choice experiments with a nationally representative sample of 1,039 U.S. consumers. Participants evaluated their willingness to pay (WTP) for salmon, venison, and morel mushrooms, presented in both wild-foraged/hunted and farm-raised forms.Results Consumers exhibited strong preferences for wild versions of all three products, with average …

Examining Light-Circadian Interactions In Regulation Of Amyloid Plaque Pathology, Melvin William King

Arts & Sciences Graduate Student Theses and Dissertations

Circadian disruption has long been appreciated as a downstream consequence of Alzheimer’s Disease in humans. However, an upstream role for circadian disruption in regulating AD pathology remains an open question. The mammalian circadian system is hierarchical, with cellular clocks – driven largely by BMAL1 – synchronized by the hypothalamic SCN (suprachiasmatic nucleus) via a combination of hormonal and synaptic cues. To assess the relative contributions of each component to Alzheimer’s Disease pathology, we crossed mice harboring either microglial (Cx3cr1/Lyz2-Cre) or GABAergic (VGAT-iCre, expressed in more than 95% of the SCN) BMAL1-knockout to mouse models of Aβ aggregation (5xFAD; APP/PS1-21), which …

Targeted Delivery Of Rapamycin Via Epidermal Growth Factor Receptors In Pancreatic Cancer Cells Inhibits Cell Proliferation And Induces Apoptosis, Adeolu S. Oluremi, Christofer Baldwin, Nickolas L. Gustavision, Mujeebat Bashiru, Adeniyi Nsiah Oyebade, Noureen Siraj, Raj Raghavendra Rao, N. Ali

Biomedical Engineering Faculty Publications and Presentations

Pancreatic cancer (PC) is aggressive and deadly; most PC cells overexpress an epidermal growth factor receptor (EGFR). EGFR-targeting peptide (GE11) was conjugated to gold nanoparticles (AuNPs) for delivering rapamycin (Rap), an mTOR inhibitor that reduces proliferation and induces apoptosis. Biophysical techniques confirmed the conjugation. Rap loading efficiency was 41.5%, with sustained release at a lower pH. Conjugates showed higher uptake in PANC-1 cells than unmodified AuNPs. It also inhibited proliferation and induced apoptosis via pathways involving reactive oxygen species, mitochondrial damage, and caspase activation. This approach represents a promising nanoparticle-based therapeutic strategy targeting PC cells, utilizing GE11-EGFR interactions to minimize …

A Phase I Trial Of Evorpacept, Lenalidomide And Rituximab For Patients With B-Cell Non-Hodgkin Lymphoma, Paolo Strati, Lei Feng, Andrey Tyshevich, Darya Shavronskaya, Julia Alesse, Noel English, Elizabeth Sheehan, Nikita Syzrantsev, Alexander Nesmelov, Tony Z Zhuang, Dai Chihara, Jason R Westin, Sairah Ahmed, Luis E Fayad, Jared Henderson, Kylie Dent, Elizabeth Mcchesney, Sattva S Neelapu, Christopher R Flowers

Faculty, Staff and Student Publications

Introduction: SIRPα+ macrophages can mediate resistance to lenalidomide and rituximab (R2) in patients with B-cell non-Hodgkin lymphoma (B-NHL). Evorpacept (ALX148) is a novel CD47 blocker that abrogates interactions between lymphoma cells and SIRPα+ macrophages.

Methods: Adult patients with B-NHL who had received at least 2 prior lines of systemic therapy were included in this single arm phase I study (NCT05025800). Evorpacept was administered intravenously (IV), in a 28-day cycle, until progression, at two dose levels (DL): 30 mg/Kg on day (D) 1 and D15 (DL1), or 60 mg/Kg on day 1 (DL2); rituximab 375 mg/m2 IV was given …

Miniature Carnation Cut Flower Production In Utah, Kellie Webb, Melanie Stock, Benjamin Scow, Cheyenne Reid, Victoria Xiong, Claudia Nischwitz, Nick Volesky

All Current Publications

Carnation is one of the most popular cut flowers worldwide and has been grown for centuries. The delicate, fringed petals of miniature carnation have a spicy, clove-like scent and are often used commercially in potpourri and perfume. Florists value the scent, versatility in both large and small design work, long vase life, and broad range of colors, which include yellow, orange, pink, white, red, and purple. While traditionally grown under cover in regions like Colombia and Ecuador, carnation may be a viable cut flower for U.S. Intermountain West growers, as plants tolerate frost and grow well in both cool and …

Effects Of Mating System And Adaptedness On The Evolution Of Fitness And Mtdna Copy Number In Mitonuclear Mismatched C. Elegans, Zachary P. Dietz, Devshuvam Banerji, Jennifer A. Sullins, Brent W. Bever, Stephen F. Christy, Ulfar Bergthorsson, Vaishali Katju, Suzanne Estes

Biology Faculty Publications and Presentations

Metabolic functioning in nearly all eukaryotes relies on molecular machinery dual-encoded by mitochondrial DNA (mtDNA) and nuclear DNA (nDNA) genomes. The two genomes have sustained an extraordinary degree of cooperation across evolutionary time, preserving the capacity for indispensable processes including oxidative phosphorylation and ATP production, which in turn influence many fitness-related traits. How this cooperation is maintained when one member of the pair is debilitated by deleterious mutation is poorly understood, as is the influence of mutation location (mtDNA or nDNA), mating system, or the potentially compensatory effects of mtDNA copy number changes on the process. We asked whether and …

Transformation With Genes To Improve Lipid Production And Barcoding To Identify Unknown Algal Species, Michelle T. Rada

Theses and Dissertations

With the rising demand for alternative fuels, algal-based biofuels are a promising, sustainable source that meets commercial interest while producing lower greenhouse gas emissions compared to fossil fuels. This thesis explored two studies, evaluating microalgae for their future potential in lipid yield for biofuel production.

The first study involved developing a cost-efficient electroporation protocol that allowed for the genetic transformation of Chlamydomonas reinhardtii using the pChlamy_4 vector, which supports future cloning of genes to enhance lipid production. Parameters affecting DNA uptake and integration into algal genomes were studied: the effect of cell density, the settings of the electroporation device, and …

Dual Action Dynamics: Assessing Hypopharyngeal Cancer Cell Survival Using Combination Chemotherapy, Luke Nieman

Biology Summer Fellows

Targeting glucose metabolism is an attractive strategy for cancer treatment, as cancer cells rely more heavily on glycolysis than oxidative phosphorylation, a phenomenon known as the Warburg Effect (Wang et al 2015). Reversing this metabolic reprogramming can impair cancer cell proliferation. NG52, a small-molecule inhibitor of PGK1, has been shown to effectively reverse the Warburg Effect and reduce cancer cell viability (Wycliffe et al. 2007). Paclitaxel, commonly Taxol, is a chemotherapeutic agent that disrupts mitotic spindle function, causing mitotic arrest and apoptosis (Markman et al 2005). In this study, we aimed to evaluate the individual and combined effects of NG52 …

Human Ipsc-Based Breast Cancer Model Identifies S100p-Dependent Cancer Stemness Induced By Brca1 Mutation, Jingxin Liu, Cai Zhao, Jiahao Chen, Pengguihang Zeng, Qingjian Li, Ranran Dai, Xingqiang Lai, Wenqian Song, Jianing Chen, Xixi Zhu, Xinyi Liu, Jun Sun, Jia Wang, Peihang Fang, Tengfei Wang, Wenjie Chen, Diana Guallar, Nan Cao, Jianli Zhao, Shicheng Su, Andy Peng Xiang, Yi Arial Zeng, Jie Li, Junchao Cai, Dung-Fang Lee, Jinxin Bei, Yongliang Huo, Hai Hu, Shengbao Suo, Dong-Feng Huang, Jin Bai, Junjun Ding

Faculty, Staff and Student Publications

Breast cancer is the most common malignancy in females and remains the leading cause of cancer-related deaths for women worldwide. The cellular and molecular basis of breast tumorigenesis is not completely understood partly due to the lack of human research models which simulate the development of breast cancer. Here, we developed a method for generating functional mammary-like cells (MCs) from human-induced pluripotent stem cells (iPSCs). The iPSC-MCs closely resemble human primary MCs at cellular, transcriptional, and functional levels. Using this method, a breast cancer model was generated using patient-derived iPSCs harboring germline