Digital Commons Network^™

State Case Count As An Indicator Of Successful Multistate Outbreak Investigation, Lorisa M. Pennell

Capstone Experience: Master of Public Health

Background/Objectives: Shiga-Toxin producing Escherichia coli (STEC) is a contaminant found in multiple food types and causes multistate outbreaks due to the industrialization of food production. In this report I examine the differences in outbreaks categorized by the regulating agency responsible in outbreak prevention and compare outbreak case counts when organized by positive outbreak investigation and response outcomes.

Methods: STEC Multistate outbreak data was obtained from the Center for Disease Control’s National Outbreak Reporting System (NORS) for outbreaks occurring from 2006-2023. Outbreaks were organized into those regulated by either the US Food and Drug Administration (FDA) or the US Department of …

Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer T. Mullen

Undergraduate Honors Capstone Projects

The emergence of avian Influenza A (H7N9) virus strains in humans poses a significant threat to human health globally due to their pandemic potential, limited human immunity, and lack of virus-specific interventions. This capstone aims to address some of the limitations in antiviral research regarding Influenza A H7N9 virus by adapting two low-pathogenic virus strains A/Anhui/1/2013 (H7N9) and A/Shanghai/1/2013 (H7N9) to BALB/c mice through sequential passaging through lung tissue. Following 12 serial passages, increased virulence was observed as evidenced by increased virus replication, increased weight loss, and increased mortality in infected mice. Titration of the initial non-adapted virus isolates were …

Navigating The Challenges Of Coccidioides Surveillance: Insights From The Environment Of El Paso County, Texas, Tanzir Hossain

Open Access Theses & Dissertations

Coccidioidomycosis, or Valley fever, is a fungal infection resulting from the inhalation of spores from the Coccidioides species (C. immitis and C. posadasii), native to arid areas of the Western Hemisphere. Coccidioidomycosis presents considerable health hazards and can be fatal. It affects between 206,000 and 360,000 individuals annually, leading to 700 to 1,100 fatalities in the United States alone. In response to these issues, extensive research has concentrated on therapeutic goals for this infectious disease. Nonetheless, more limited research has focused on the environmental detection of its causative fungi, which is crucial for forecasting treatment needs and formulating preventive strategies. …

Investigating Self-Healing Mechanisms In Host-Pathogen Interactions: A Literature Review Of Macrophage Functions In Tuberculosis, Blessing Savusa

Open Access Theses & Dissertations

Tuberculosis (TB) remains a leading cause of mortality, particularly in underdeveloped regions. Mycobacterium tuberculosis (Mtb) is regarded as one of the most successful pathogens due to its ability to evade host immunity and persist within granulomas. Macrophages are the first-line host defenders that engulf Mtb into phagosomes, which initiates lysosomal activity for degradation. However, the virulence factor of Mtb is able to inhibit phagolysosome fusion. The host immune system forms granulomas to contain the mycobacterium at the site of inflammation. This, in turn, causes a necrotic state and causes tissue damage from the prolonged inflammation. Studies suggest that the host's …

Intratumoral Fibrin As A Novel Immunomodulatory Factor In Pancreatic Ductal Adenocarcinoma, Mazharul Karim

Open Access Theses & Dissertations

In-situ coagulation is a common clinical feature of pancreatic ductal adenocarcinoma (PDAC) that presents a highly thrombotic and crosslinked insoluble fibrin (x-fibrin)-laden tumor stroma (FibTS). However, the specific impact of FibTS on immune cells behavior remains largely unexplored in this poorly infiltrated tumor. We aimed to elucidate how FibTS in PDAC regulates immune cell infiltration, polarization, and crosstalk that favors immunosuppressive microenvironment and tumor growth. We characterized human PDAC tissues by multiplex immunostaining and checked the spatial distribution of immune cells based on the presence of x-fibrin. We investigated how FibTS influences the infiltration of tumor-associated macrophage (TAM) and T-cell …

Variation In Stress Response Regulation Across Natural Isolates Of Saccharomyces Cerevisiae, Crystal Christine Crook

Graduate Theses and Dissertations

The stress response of Saccharomyces cerevisiae is a valuable area of study for understanding cellular adaptation mechanisms that govern survival in fluctuating environments. This dissertation investigates stress response regulation in yeast through the roles of Hog1 mitogen-activated protein kinase (MAPK) and the post-transcriptional regulator Mkt1, a key post-transcriptional regulator, in diverse wild yeast isolates. Hog1 is traditionally known for its central role in osmotic stress regulation in the high-osmolarity glycerol (HOG) pathway. This study expands the functional scope of Hog1 to include oxidative and ethanol-induced stress responses.

In parallel, we investigate post-transcriptional regulation of stress responses by the Mkt1 interactome, …

The Efficacy Of Foam-Based Hand Sanitizers For The Control Of Enveloped And Non-Enveloped Virus Transmission, Francis Torko

Graduate Theses and Dissertations

Infectious disease transmission due to viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza virus, human noroviruses (HuNoV), and hepatitis A, can occur via person-to-person contact or contaminated surfaces. Effective hand hygiene, including hand washing and use of hand sanitizers, is considered a critical tool for the control of infectious disease transmission, as recommended by the United States Centers for Disease Control and Prevention and the World Health Organization. However, while research on hand washing efficacy is well-documented, research on hand sanitizer efficacy, particularly for foam-based products, remains limited. This research addressed critical gaps in the area of …

Characterization Of Streptococcus Gallolyticus: An Emerging Pathogen Causing Turkey Streptococcosis, Latasha S. Gray

Graduate Theses and Dissertations

During the past five years, there has been an increased occurrence of Streptococcus gallolyticus in young turkey poults. This emerging pathogen previously affected turkey flocks without any indication of disease prior to acute mortality. However, recently there has been two cases of neurological presentation associated with S. gallolyticus subsp. pasteurianus that are unique to traditional disease presentation in turkey poults. The objectives of the present dissertation were to compile data associated with the two neurological cases and compare different S. gallolyticus strains as well as pathogenic Enterococcus cecorum to the neurological strains. The two neurological cases occurred between July and …

Donor Age Matters For Haploidentical Hct Patients Even After Adjusting For Hla Factors: A Machine Learning Approach, Rohtesh S Mehta, Rodney Sparapani, Tao Wang, Stephen Spellman, Stephanie J Lee, Effie W Petersdorf

Faculty, Staff and Student Publications

No abstract provided.

Rna Sculpting By The Primordial Helix-Clasp-Helix-Strand-Loop (Hch-Sl) Motif Enforces Chemical Recognition Enabling Diverse Kh Domain Functions, John A Tainer, Susan E Tsutakawa

Faculty, Staff and Student Publications

In all domains of life, the ancient K homology (KH) domain superfamily is central to RNA processes including splicing, transcription, posttranscriptional gene regulation, signaling, and translation. Proteins with 1 to 15 KH domains bind single-strand (ss) RNA or DNA with base sequence specificity. Here, we examine over 40 KH domain experimental structures in complex with nucleic acid (NA) and define a novel Helix-clasp-Helix-Strand-Loop (HcH-SL) NA recognition motif binding 4 to 5 nucleotides using 10 to 18 residues. HcH-SL includes and extends the Gly-X-X-Gly (GXXG) signature sequence "clasp" that brings together two helices as an ∼90° helical corner. The first helix …

Pd-L1 Testing, Treatment Patterns, And Clinical Outcomes Among Patients With Metastatic Nsclc At An Academic Medical Center, 2017-2021, Mehmet Altan, Dawen Sui, Cai Xu, George R Simon, Saliha T Sulihem, Donna Malveaux, Darcy Ponce, Waree Rinsurongkawong, Vadeerat Rinsurongkawong, J Jack Lee, Jianjun Zhang, Don L Gibbons, Ara A Vaporciyan, John V Heymach, Melissa L Santorelli, Thomas Burke, Loretta A Williams

Faculty, Staff and Student Publications

Introduction: Targeted therapies and immune checkpoint inhibitors (ICIs) have revolutionized the management of metastatic non-small cell lung cancer (NSCLC) over the past decade.

Methods: This single-center observational study was conducted to describe programmed death-ligand 1 (PD-L1) testing, choice of therapy, and outcomes for adult patients with stage IV NSCLC initiating first-line therapy from 2017 through 2020, with follow-up through June 2021. Patient characteristics and study assessments were described according to four histomolecular subtypes, defined by histologic characteristics and availability of standard-of-care therapies for molecular subgroups at the time of study conduct.

Results: Of 507 eligible patients with metastatic NSCLC, 85 …

Parasitic Effects Of Amoebidium Appalachense On Daphnia Magna Heart Rate, Hopping Frequency, And Metabolic Rate, Vanessa Wise

Honors Program: Senior Projects (Public)

Daphnia magna are a crustacean that inhabits multiple different types of freshwater ecosystems and thus is plays a part in many different types of interspecies interactions, one of which is parasitism. Amoebidium appalachense is a Mesomycetozoean parasite of aquatic arthropods that colonizes the antenna of D. magna. Previous research has shown that A. appalachense infection will reduce the fecundity and increase mortality rate of D. magna but how it causes these effects is unknown. To determine if A. appalachense causes these effects on D. magna by either stealing nutrients inside the D. magna like a tick or by compromising …

Evidence Based Research Review Of Remote Independent Chemotherapy/Immunotherapy Verification, Katrina Perkins, Hannah Holder, Marie St. Clair

Covenant Nurses Week 2025

No abstract provided.

Factors Related To The Incidence Of Hypertension Among People Above 18 Years At The Cibatu Public Health Center, Bekasi Regency, Verizka Azra, Yustiyani Yustiyani, Minsarnawati Minsarnawati, Catur Rosidati

Indonesian Journal of Public Health Nutrition (IJPHN)

Hypertension is a non-communicable disease that still becomes a public health issue. The percentage of hypertension cases at Cibatu Public Health Center increased from 31.7% in 2021 to 37% in 2022. Based on a preliminary study, hypertension was found to be the most common non-communicable disease at Cibatu Public Health Center. This study aimed to identify factors associated with the incidence of hypertension among individuals aged 18 years and older at Cibatu Public Health Center, Bekasi Regency, in 2024.We conducted a cross-sectional study in May involving 121 respondents. This study used standardized questionnaires that had been validated in previous research, …

Exploring The Non-Communicable Disease Burden In Indonesia – Findings From The 2023 Health Survey, Akifa Laila Rusyda

Indonesian Journal of Public Health Nutrition (IJPHN)

The present study rigorously examines the correlation between obesity, high-risk dietary consumption, and unhealthy lifestyles with the prevalence of non-communicable diseases (NCDs) in Indonesia. The study employed a cross-sectional study design using secondary data from the 2023 Indonesian Health Survey, analyzing 38 units (n) representing Indonesia's provinces in 2023. The correlation analysis, conducted using Pearson's test with a significance level of 95% (α<0.05), revealed significant regional disparities in the prevalence of NCDs, dietary consumption, and lifestyle factors across Indonesia. Moreover, the findings reveal a statistically significant positive correlation between obesity, high-risk food consumption, and lifestyle risk factors with the prevalence of several NCDs, including diabetes mellitus, heart disease, hypertension, and chronic kidney disease. These results underscore the potential impact of our research on public health in Indonesia. The results highlight also the urgent need for targeted public health initiatives, such as policies promoting healthier food choices, controlling the food environment, encouraging physical activity, and supporting smoking cessation to mitigate the growing burden of NCDs in Indonesia.

The Effects Of Prescribed Medications On Depressive Symptoms And Neurocognitive Performance In People With Hiv, Asante R Kamkwalala, Avery Matthews, Ankita Garg, Upal Roy, Qing Ma, Maile Karris, Erin Sundermann, Ronald J Ellis, Patricia K Riggs, Mattia Trunfio, Jennifer Blanchard, David J Moore, Leah H Rubin, Scott L Letendre

Faculty, Staff and Student Publications

Background: Alterations in brain function and structure, such as depression and neurocognitive impairment, continue to occur in people with human immunodeficiency virus (HIV, PWH) taking suppressive antiretroviral therapy (ART). The lifespan of PWH has improved but the healthspan remains worse than people without HIV, in part because of aging-related diseases. As a result, polypharmacy is common and increases the risk of drug-drug interactions and adverse reactions.

Methods: This cross-sectional project investigated the relationship between 7 medication-related metrics (including anticholinergic burden), depressive symptoms, and neurocognitive performance in 491 PWH at a single center in the United States. All participants were taking …

Bibliometric Analysis Of Research Trends Related To Human Immunodeficiency Virus., Madyson E. Johnson, Kaitlyn R. Coffman, Valerie Rivas, Elizabeth Barrett

ATU Scholars Symposium

Human immunodeficiency virus poses major health burden across the globe including the United States of America and has its zoonotic origin from the Simian Immunodeficiency viruses of sooty mangabey monkey and chimpanzee in Africa. Epidemiological trends indicate that even though the mortality rates for HIV are falling globally, the rates of new cases have been rising since 2010 in several countries including the USA. An estimated 0.5% of the human population is currently infected with the virus with countries like USA, India, Nigeria and South Africa carrying the highest burden of the disease. Host receptors such as CD4 and coreceptors …

Cloning The Yersinia Enterocolitica Alkaline Phosphatase For An Affinity Chromatography Ligand, Flavio E. Arellano

ATU Scholars Symposium

Alkaline phosphatase (ALP) is an enzyme responsible for the dephosphorylation of extra-cytoplasmic molecules. Conversion to a neutral molecule enhances their ability to pass through cell membranes. When microbes such as Yersinia enterocolitica are ingested, they are exposed to large changes in pH level. These changes range from ~pH6.5 in the oral cavity to pH 2 in the stomach and pH 8 in the mid-intestine. The ability of microorganisms to survive this change in pH it largely dependent on their ability to change which proteins are expressed at differing pH levels or their ability to modify those proteins to meet the …

Comparing Parasitism And Resistance To Flow In Two Populations Of Elimia Virginica, Morgan G. Hicok, Abbie Tomba, Kate Green

Departmental Honors & Graduate Capstone Projects

Snails are important in freshwater ecosystems as primary consumers and are good indicators of the stability of a habitat. Trematodes are parasitic flatworms that infect freshwater snails and can cause changes in metabolic function and growth. Flow rates and water levels are now more variable due to climate change, increasing stress and dislodgment in the snail population. This study investigates how parasites, shell morphology, and native flow environment influence resistance to disturbance. Elimia populations were sampled for differences in parasitism, shell morphology, and ability to withstand high flow environments in two different rivers. Sample sites at the Potomac River were …

Metabolic And Microbial Crossroads: Sodium-Glucose Cotransporter-2 Inhibitors And Urinary Tract Infections In (Asian) Diabetes Care, Rob E. Carpenter

Human Resource Development Faculty Publications and Presentations

The global rise of type 2 diabetes, particularly in Asian populations, has led to widespread adoption of sodium-glucose cotransporter-2 (SGLT2) inhibitors for glycemic control. While effective, these agents elevate the risk of urinary tract infections (UTIs) by inducing glycosuria, which creates a nutrient-rich environment that favors uropathogen growth. Asian individuals may be disproportionately vulnerable to SGLT2 inhibitor–associated UTIs due to a confluence of factors, including lower body mass index, increased visceral adiposity, congenital urinary tract anomalies, and genetic polymorphisms that impair uroepithelial integrity and immunity. This review integrates emerging evidence on the molecular, anatomical, and immunometabolic mechanisms that underlie infection …

From Dysbiosis To Dysfunction: Investigating The Effects Of Chronic Cocaine Use On Oral Microbial Shifts And Neuropathogenesis, Douglas E. Johnson

MUSC Theses and Dissertations

Cocaine use disorder remains a significant public health challenge. However, the mechanisms by which chronic cocaine use contributes to neuroinflammation and cognitive decline are still not fully understood. Chronic non-intravenous cocaine use is associated with oral microbial dysbiosis. Although the gut-brain axis has been widely studied, the impact of cocaine-mediated shifts on the oral microbiome on systemic and neuroinflammation remains a significant yet largely unexplored gap. This dissertation investigates the hypothesis that cocaine-specifically enriched oral Streptococcus parasanguinis triggers neuroinflammation, neuropathology, and cognitive deficits. In a human study, the saliva microbiomes from chronic cocaine users exhibited reduced α-diversity, enriched Streptococcus genus, …

Investigating Genetic Regulators Of Crystal Cell Development In Drosophila Melanogaster, Andrea Feria, Nyla Walbrook, Anisa Turaeva, Rebecca Spokony

Publications and Research

Crystal cells, a subset of Drosophila melanogaster hemocytes, are important for melanization, a crucial immune response. While the genetic regulation of crystal cell develop remains incompletely understood, this study investigates the functions of five candidate genes: ACXB, ACXA, Gp210, CG4390, and Cyp4s3; identidied through previous genome-wide association studies. Using the Gal4/UAS system along with RNA interference (RNAi), we exclusively knocked down each gene in third instar larvae and quantified crystal cell populations following heat shock-induced melanization. Our results imply that the knockdown of ACXB and ACXA significantly increased crystal cell counts in both sexes, suggesting these genes either act as …

Passive Integrated Transponders: An Effective Marking Technique For Individual Identification Of Anaxyrus Fowleri?, Emily Leprell

Honors College Theses

With the rise of fungal disease such as Chytridiomycosis, known as chytrid, causing massive population declines for many species of frogs and toads (order Anura), specific conservation strategies are needed now more than ever. In many cases, being able to mark and identify individuals is a necessary step in developing an effective conservation plan. Individual identification allows researchers to obtain accurate population estimates, which are needed to test the progress and validity of conservation management decisions. However, due to their unique life history, including cutaneous respiration and a dichotomous lifestyle, anurans are among the most difficult wildlife species to …

Impacts Of Climate Change On Cannibalism And Disease Transmission In The Fall Armyworm, Kale Rougeau

LSU Master's Theses

Climate change may alter host-pathogen dynamics in terrestrial ecosystems due to rising global temperatures and carbon dioxide levels. Here, we examined cannibalism and disease transmission using the fall armyworm (Spodoptera frugiperda), an invasive agricultural pest, and its species-specific lethal baculovirus, Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), under various potential challenges of climate change. We used three temperature treatments (cooler, optimal, warmer) based on the fall armyworm’s thermal performance curve to simulate a changing climate and four artificial diets with various protein-to-carbohydrate ratios (low, equal, high, standard) to manipulate resource quality from altered carbon dioxide levels. We found that higher …

From Ancient Wisdom To Modern Medicine: The Cultural And Therapeutic Significance Of Ficus Carica L. (Moraceae) Extracts In Colorectal Cancer Treatment, Esra Hacimüftüoğlu, Azizeh Shadidizaji, Kağan Tolga Ci̇ni̇sli̇, Songül Karakaya

Turkish Journal of Agriculture and Forestry

Figs, mentioned in the Torah, the Bible, and the Quran, are of historical and cultural significance and have been used for medical treatment, prompting extensive research. Colorectal cancer (CRC), the second leading cause of cancer-related death worldwide, is expected to significantly increase in terms of mortality rates by 2035, with its progression closely linked to inflammatory processes. Cyclooxygenase 2 (COX-2), an enzyme encoded by the Ptgs2 gene, plays a key role in these processes by converting arachidonic acid into prostaglandin E2 (PGE2), a mediator of inflammation. In the present study, the potential of natural antiinflammatory compounds, specifically fumaric acid and …

Enriched Pathways In Gut Microbiome Predict Response To Immune Checkpoint Inhibitor Treatment Across Demographic Regions And Various Cancer Types, Xunhui Cai, Jennifer Y Cho, Lijun Chen, Yufeng Liu, Fenghu Ji, Katia Salgado, Siyi Ge, Dehua Yang, Hui Yu, Jianbo Shao, P Andrew Futreal, Boris Sepesi, Don Gibbons, Yaobing Chen, Guoping Wang, Chao Cheng, Meng Wu, Jianjun Zhang, Ansel Hsiao, Tian Xia

Faculty, Staff and Student Publications

Understanding the effect of gut microbiota function on immune checkpoint inhibitor (ICI) responses is urgently needed. Here, we integrated 821 fecal metagenomes from 12 datasets to identify differentially abundant genes and construct random forest models to predict ICI response. Gene markers demonstrated excellent predictive performance, with an average area under the curve (AUC) of 0.810. Pathway analyses revealed that quorum sensing (QS), ABC transporters, flagellar assembly, and amino acid biosynthesis pathways were enriched between responders (R) and non-responders (NRs) across 12 datasets. Furthermore,

Associations Between Linguistic Factors And Vaccine Attitudes In A Hispanic Immigrant Population In The United States, Jamie Jensen

ScholarsArchive Data

Data are results of a nationwide survey administered by Qualtrics to Hispanic Immigrants concerning HPV vaccine uptake and hesitancy factors.

Designing An Optimal Thermal Preference Arena For Southern Cricket Frog (Acris Gryllus) Juveniles, Eleanor E. Briggs, David M. Phillips Jr.

Honors Theses

Climate change has been altering ecosystems across the world at a substantial rate, disrupting the delicate ecophysiological relationships between ecosystems and the organisms that live in them. Ectotherms, such as amphibians, rely on external temperatures to behaviorally thermoregulate, making it especially imperative to study their thermal biology. Thermal preference, which assesses the environmental temperature an organism would seek out in the absence of external stimuli, provides valuable information about an amphibian’s thermal biology and can be used to predict the effects of climate change on a species or population. A commonly used method to measure thermal preference is an arena …

Early Detection Of Colon Cancer Through Colonoscopy: Analysis Of Tumor Size And Resection Length, Alyssa Haddock

Honors Theses

Colon cancer remains a leading cause of cancer-related deaths in the United States, with early detection critical for improved outcomes. Colonoscopy is a primary screening tool that can reduce incidence rates through the identification and removal of precancerous polyps. This study examines how undergoing a colonoscopy prior to diagnosis impacts two key surgical outcomes: tumor size and colon resection length. Data were collected from 70 de-identified patients who underwent colon resection for colon cancer between 2019 and 2024 at two regional hospitals in East Texas: Hunt Regional Medical Center and Mother Christus Frances Hospital. Using pathology reports, tumor size and …

The Importance Of Wild Birds, Janice H. Cox

Nexus – UNEP – Animal Welfare, Environment, Sustainable Development

Wild birds are important in their own right, for their contribution to human wellbeing, and for their ecological value, as part of the “web of life”. Birds are sentient beings with complex thinking and social behaviors, and the ability to experience positive and negative feelings, including suffering. The ways in which birds contribute to the functioning of the world's ecosystems include: pest control, pollination, cleaning up nature, spreading seeds, keeping coral reefs alive and transforming landscapes. Yet wild bird populations are declining dramatically – with one in eight bird species threatened with extinction. Birds are one of the most important …