Digital Commons Network^™

Status Of Cardiac Transplantation With A Report Of The First Year's Experience At Henry Ford Hospital, Fraser M. Keith, Donald J. Magilligan Jr., Jeffrey B. Lakier, Conrad Drost

Henry Ford Hospital Medical Journal

Cardiac transplantation is now widely accepted as effective treatment for selected patients with end-stage heart disease. Improvements in immunosuppressive treatment and monitoring in the past 15 years have resulted in impressive one-year and five-year survival rates of 80% and 60%. In general, survivors have enjoyed a considerably improved quality of life. A cardiac transplantation program was initiated at Henry Ford Hospital in 1985, and a total of 15 patients received heart transplants in the first 12 months. Fourteen patients are currently alive and well at various stages posttransplant. This early clinical success has prompted the consideration of both combined heart-lung …

Amplification And Chromosomal Dispersion Of Human Endogenous Retroviral Sequences, Paul E. Steele, Malcolm A. Martin, Arnold B. Rabson, Theodore Bryan, Stephen J. O'Brien

Biology Faculty Articles

Endogenous retroviral sequences in humans have undergone amplification events involving both viral and flanking cellular sequences. We cloned members of an amplified family of full-length endogenous retroviral sequences. Genomic blotting, employing a flanking cellular DNA probe derived from a member of this family, revealed a similar array of reactive bands in both humans and chimpanzees, indicating that an amplification event involving retroviral and associated cellular DNA sequences occurred before the evolutionary separation of these two primates. Southern analyses of restricted somatic cell hybrid DNA preparations suggested that endogenous retroviral segments are widely dispersed in the human genome and that amplification …

The Effects Of Certain Oral Bacteria On The In Vitro Growth Of Bacteroides Gingivalis, Steven G. Morrow

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of nine gram positive and grain negative cocci and bacilli, which are indigenous to the oral cavity, on the in vitro growth of Bacteroides gingivalis was examined in this study. A pure culture of B. gingivalis is was obtained from the American Type Culture Collection. The nine oral test organisms were Streptococcus mitis, Streptococcus mutans, Streptococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Corynebacteriurn hofmanii, Lactobacillus acidophilus, Branhamella catarrhalis, and Escherichia coli. The effect on the growth of B. gingivalis of the nine test organisms was evaluated on a solid medium by use of …

Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin

Nebraska Center for Virology: Faculty Publications

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

The Incidence Of Cryptosporidial Infections In Nebraska Dairy Calves, Douglas Lee Varner

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Within the last decade a new etiologic agent has been identified as a possible cause of diarrhea in humans and domestic animals—that being the protozoan parasite Cryptosporidium. The purpose of this study was to determine the incidence and distribution of Cryptosporidium in the state of Nebraska, to determine if Cryptosporidium occurs more frequently in association with other enteropathogens and to determine about the relation of scouring in the calves to infection with Cryptosporidium. A total of 620 fecal samples from 334 dairy calves from 71 herds in Nebraska were examined for cyptosporidial oocysts using the Sheather’s sugar flotation …

Polymorphism Of Eimerian Oocysts: A Dilemma Posed By Working With Some Naturally Infected Hosts, Brent B. Parker, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Morphological variation of sporulated oocysts within individual eimerian species is well documented (Joyner, 1982. In Biology of the Coccidia, P. L. Long (ed.). University Park Press, Baltimore, pp. 35-62). In some cases, oocysts of a single eimerian species are known to vary in size by as much as 40% during patency (Duszynski, 1971, Journal of Parasitology 57: 948-952). During a survey to determine the prevalence of coccidiosis in sandhill cranes (Grus canadensis) wintering in New Mexico (Parker and Duszynski, 1986, Journal of Wildlife Diseases 21: 25-35), marked polymorphism was observed among sporulated oocysts of Eimeria reichenowi. …

Intrauterine Device (Iud) Associated Pathology: A Review Of Pathogenic Mechanisms, Waldemar A. Schmidt, Karmen L. Schmidt

Scanning Electron Microscopy

This paper summarizes our studies of IUD-related disease with those previously published by others. Our data are based upon 51 IUDs and 42 index cases of IUD-related disease demonstrating specific processes. Gross, dissecting microscope, scanning electron microscope and X-ray microanalysis examinations were made of selected IUDs and associated tissues. Tissue associated with the IUDs revealed inflammation in 59.4%, calcific material in 6.3% and no abnormality in 34.4%. JUD-associated tissue responses were accompanied by changes of the IUD; these changes involved deposition of substances upon the IUD surface and degradation of the JUD itself. Disintegration of the IUD, its string or …

An Immunochemical Analysis Of Alcoholic Hyaline And Its Relationship To Keratin Intermediate Filaments, David L. Drexler

Loma Linda University Electronic Theses, Dissertations & Projects

Alcoholic Hyaline (AH) and keratin intermediate filaments possess ultrastructural, biochemical and immunochemical similarities. This study compares human AH, human stratum corneum keratin (HSCK) and normal human liver cytokeratin (HCK) by quantitative immunochemical means, using polyclonal as well as monoclonal antibodies. Enzyme-linked immunosorbent assays (ELISAs) were employed, as well as "immunoblotting" and immunocytochemical techniques. HCK was isolated using Triton X-100 and "high-salt” buffers and exhibited seven major polypeptide bands on SDS-PAGE (41-58 kd). Antisera were made toward HCK and the two major HCK bands. Other immunochemicals included antisera toward AH, the three major AH bands, and HSCK, as well as 3 …

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments.

In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay …

Viruses In Water, Nasrin Mohammadpour Daryaee

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

The use of water by man, plants and animals is universal. Without it there can be no life. Every living thing requires water. Man can live nearly two months without food, but can live only three or four days without water. In our homes, whether in the city or in the country, water is essential for cleanliness and health. Water can be considered as the principal raw material and the lowest cost raw material from which most of our farm produce is made. It is essential for the growth of crops and animals and is a very important factor in …

Survey Of Parasite Control Practices In Sheep And Cattle, J R. Edwards, R V R Gwynn, R A. Love, Richard Thomas Norris, G Dalton-Morgan, Brown Besier

Technical Bulletins

Farms in the Albany, Esperance, Jerramungup and Katanning districts were surveyed to determine parasite control strategies currently used, to evaluate the adoption of recommended control practices and to detect areas of ineffective parasite control. For cattle, the number of drenches given in the low rainfall zone was higher than the number given in the high rainfall zone.

Tropism Of Sheep Lentiviruses For Monocytes: Susceptibility To Infection And Virus Gene Expression Increase During Maturation Of Monocytes To Macrophages, Howard E. Gendelman, Opendra Narayan, Suzanne Kennedy-Stoskopf, Peter G. E. Kennedy, Zahra Ghotbi, J. E. Clements, Jeff Stanley, Gholam Pezeshkpour

Nebraska Center for Virology: Faculty Publications

Visna lentiviruses have a natural tropism for cells of the macrophage lineage of sheep and goats, but virus replication in these cells in vivo is restricted so that only small quantities of virus are produced. One restricting factor suggested in previous studies is that virus replication is dependent on the maturity of the cells: the more mature the cell, the less restrictive the replication of the virus. Since monocytes in peripheral blood are precursors of macrophages, we investigated the effect of cell maturation on virus replication under limited control conditions in vitro by inoculating blood leukocytes with virus and retarding …

Disease Incidence Of Demersal Finfishes From The Southern And Western Branches Of The Elizabeth River, Virginia, Randal D. Owen

Biological Sciences Theses & Dissertations

Fishes from the industrialized environs of the Elizabeth River, Virginia were surveyed for external evidence of disease during June through October 1984. A highly significant (p < 0.001) incidence of disease was found in demersal fishes collected by otter trawl from a site in the industrially contaminated Southern Branch compared to a less contaminated Western Branch site. Cataracts, fin erosion and dermal lesions were the most commonly occurring anomalies in the Southern Branch finfish.

Static, solid-phase toxicity tests employing sediment collected from the Southern Branch site resulted in an immediate toxic stress in the spot, Leiostomus xanthurus, which resulted in 100% mortality within 24 hours, compared with no mortalities in control systems. Spot exposed to control, i.e., pollution free, and diluted Southern Branch sediments innoculated with the pathogenic bacterial species, Vibrio anguillarium, developed significant (p ≤ 0.020) cases of fin erosion at a mean total bacterial community density …

Conserved Chromosomal Positions Of Dual Domains Of The Ets Protooncogene In Cats, Mice, And Humans, Dennis K. Watson, Mary J. Mcwilliams-Smith, Christine Kozak, Roger Reeves, John Gearheart, Michael F. Nunn, William Nash, John R. Fowle Iii, Peter Duesberg, Takis S. Papas, Stephen J. O'Brien

Biology Faculty Articles

The mammalian protooncogene homologue of the avian v-ets sequence from the E26 retrovirus consists of two sequentially distinct domains located on different chromosomes. Using somatic cell hybrid panels, we have mapped the mammalian homologue of the 5' v-ets-domain to chromosome 11 (ETS1) in man, to chromosome 9 (Ets-1) in mouse, and to chromosome D1 (ETS1) in the domestic cat. The mammalian homologue of the 3' v-ets domain was similarly mapped to human chromosome 21 (ETS2), to mouse chromosome 16 (Ets-2), and to feline chromosome C2 (ETS2). …

Two New Species Of Ascometra Kholodkovskii, 1912 (Cestoda: Paruterinidae) From The Kori Bustard, Choriotis Kori (Burchell), In Kenya, Ann M. Adams, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Ascometra choriotidis n. sp. and Ascometra scheuermani n. sp. are described from a Kori Bustard, Choriotis kori (Burchell) (Gruiformes: Otididae), captured in Kenya. The two species are distinguished from Ascometra gutterae (Baylis, 1914) and Ascometra numida (Fuhrmann, 1909) (formerly placed in the genus Octopetalum Baylis, 1914) by the much larger number of testes in each segment. Ascometra choriotidis differs from Ascometra vestita Kholodkovskii, 1912, in the smaller proportional size of the cirrus sac, which extends mediad across less than half the space between the margin of the segment and poral ventral longitudinal excretory canal. From A. vestita and A. choriotidis …

Genetic Engineering Of Livestock Improvement, Gordon E. Dickerson

World Congress on Genetics Applied to Livestock Production: 3rd (1986)

Genetic engineering requires clearly defined objectives, adequate genetic variability and effective methods of selection. In a given production-marketing System, relative importance of genetic change in alternative traits can be estimated from expected effects on cost per unit of output, using available biological and economic information. Diversity among existing breeds and their crosses is a primary and quickly usable genetic resource, and its management for future needs is a public concern. Better physiological and biochemical understanding of the genetic controls of animal development and function is important to permit more effective selection for efficiency of animal production under diverse environments. Long-term …

Columnaris Disease Of Fishes, G. L. Bullock, T. C. Hsu, E. B. Shotts Jr.

United States Fish and Wildlife Service: Publications

Columnaris disease is an acute to chronic bacterial infection that affects anadromous salmonids and virtually all species of warmwater fishes. Davis (1922), who first described the disease, named it columnaris because the causal bacterial cells seen in wet mounts of affected gills and fins were arranged in columnar aggregations. Ordal and Rucker (1944) were the first to isolate the causal organism and, based on cellular morphology, identified it as a myxobacterium. Organisms classified in the order Myxobacterales are long, thin gram-negative rods that are motile on agar media by a creeping or flexing motion. They have a life cycle composed …

G86-797 Causes Of Vaccination-Immunization Failures In Livestock, Duane Rice, E. Denis Erickson, Dale Grotelueschen

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide discusses reasons why vaccinations fail to provide immunity against disease, and how to prevent this from happening.

To comprehend the many reasons for vaccine failure, it is important to understand how animals and humans have the ability to resist infectious diseases. It is also important to know what a disease is and how it affects the animal.

According to Stedman's Dictionary, disease is an interruption, cessation or disorder of body functions, systems or organs. Diseases may be obvious even to the untrained eye, or detectable only by sophisticated testing procedures (subclinical disease). Serious irreversible damage may be …

The Significance Of Alternative Techniques In Biomedical Research: An Analysis Of Nobel Prize Awards, Martin Stephens

Experimentation Collection

No abstract provided.

The Case For The Use Of Animals In Medicine, Gary F. Merrill

Experimentation Collection

The use of animals in medical research and teaching, and the public concern this has generated is not a new issue (Visscher 1969). Ever since scientists began using animals to investigate the function of the body in health and disease, there have been those who opposed their work (Fishman and Richards 1982). Whether this controversy is cyclic is not known, but most concerned biomedical investigators agree that the opposition is here to stay. The author shares this opinion, and thus maintains that it is in the best interest of all parties to be properly educated on the issues. Only through …

Assault On Eden: Destruction Of Latin America's Rain Forests, Douglas R. Shane

Ecology Collection

In the seemingly distant world of Latin America's rain forests, man's greed and desperation have resulted in a fire which threatens to obscure our ability to observe life's poetry: The continuing destruction of the earth's tropical rain forests is one of the most serious environmental problems confronting humanity today: Intact, these vital organisms offer an understanding of the planet's past and a key to our future; destroyed, they threaten catastrophe of global consequence.

The Cruel Deception, Robert Sharpe

Experimentation Collection

With new legislation to replace the Cruelty to Animals Act 1876 near at hand, the powerful vested interest groups whose profits and livelihood depend on laboratory animals are stepping up their campaigns to ensure the survival of vivisection. Have the benefits really been so great, and can vivisection achieve major advances in our present state of health?

History shows (McKeown 1979) that the real reasons for the dramatic increase in life expectancy since the middle of the last century are improvements in nutrition, living and working conditions, hygiene and sanitation, with medical measures only having a relatively marginal effect. The …

Alternative To Current Uses Of Animals In Research, Safety Testing, And Education: A Layman's Guide, Martin L. Stephens

eBooks

No abstract provided.

Atrophic Rhinitis In Pigs, A R. Mercy

Journal of the Department of Agriculture, Western Australia, Series 4

Severe atrophic rhinitis in pigs recurred recently in Australia after an apparent absence of some 20 years. The first of these recent cases occurred in a Westerm Australian herd in late 1984 and since then six other local herds have reported the disease. Severe atrophic rhinitis has also been seen recently in South Australia.

Atrophic rhinitis is a complex disease which causes degeneration of the nasal cavity, twisting of the snout and sometimes occasional bleeding from the nose.

It occurs only in pigs and severely affected animals may have a slower growth rate.

Rhizoctonia Patch Of Cereals, G C. Macnish

Journal of the Department of Agriculture, Western Australia, Series 4

Rhizoctonia patch is now widespread within Western Australia, having been recorded on all cereals and on a wide variety of other crops and pasture plants. Although the disease-as its name implies - looks spectacular in the field, the overall reduction in crop yield is probably small. However, its increasing frequency is causing considerable concern as the disease appears to be associated with minimum or reduced tillage systems.

Species-Specific Restriction Site Polymorphism In Root-Knot Nematode Mitochondrial Dna, Thomas O. Powers, E. G. Platzer, Bradley C. Hyman

Department of Plant Pathology: Faculty Publications

Research was initiated to physically characterize the mitochondrial genomes of several Meloidogyne spp. and host-races, to address questions regarding their systematics and dispersal, and to assess the possibility of developing molecular diagnostics for these nematodes. Techniques were developed for purification and rapid detection of mitochondrial DNA from root-knot nematodes. Mitochondrial DNAs among Meloidogyne spp. were demonstrated to exhibit extensive divergence. The potential for using the rapidly diverging mitochondrial genomes as a diagnostic assay for M. incognita, M. hapla, M. arenaria, and M. javanica is discussed.

Bulletin No 4101 - Flystrike - A Manual For Its Prevention And Control, N Monzu Dr

Bulletins 4000 -

The sheep blowfly is still regarded as the Australian sheep industry's most­damaging pest. Estimates of the cost to the industry, through production losses and prevention and control measures, exceed $ I 00 million in a year. This bulletin comprises the most relevant information available on:

• Blowfly types and life cycles

• Conditions predisposing sheep to flystrike

• The mechanism of flystrike

• Methods of preventing and controlling strike.

• Current research - including the development of a forecasting system for body strike.

Recovery Of Parasite Remains From Coprolites And Latrines: Aspects Of Paleoparasitological Technique, Karl J. Reinhard, Ulisses E. Confalonieri, Bernd Herrmann, Luiz F. Ferreira, Adauto J. G. De Araujo

Department of Anthropology: Faculty Publications

Standard techniques for the analysis of prehistoric soils have not been devised. It is unlikely that any single technique is applicable to all types of fecal remains. This is due to various environmental conditions which effect the preservation of helminth ova. In general, gravitational sedimentation is a useful technique for isolating helminth eggs and larvae from coprolites. Latrine soils pose greater problems for helminthological examination. Although various clinical techniques have been successfully utilized in soil study, it is important to remember that some latrine soils have not yielded helminth eggs to any clinical technique. Consequently the paleoparasitologist must be ready …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Small Mammal Community Structure And Composition In The Cerrado Province Of Central Brazil, Michael A. Mares, Kristina A. Ernest, Donald D. Gettinger

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Community structure, macrohabitat selection, and patterns of species co-occur- rence were examined during a 14-month study of small mammals in the Cerrado Province of central Brazil. Data were collected from mark-recapture grids in brejo and gallery forest, and from live-trapping and specimen collection in all habitat types within cerrado (campo limpo, campo sujo, cerrado [s.s.], cerradão, brejo, valley-side wet campo, and gallery forest). Gallery forest supported the highest species richness, most complex vertical distribution of species, highest level of trophic diversity, and highest macroniche diversity. Degree of habitat selection varied widely. All habitat types supported both rodents and marsupials, although …