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Evaluation Of Sustainability Assessment Post Changes In The Management Of The Natural Reserve To The Natural Tourism Park In Kamojang Area, West Java, Indonesia, Purna Hindayani, Alnidi Safarach Bratanegara, Intan Adhi Perdana Putri, Zuzy Anna, Armandha Redo Pratama

Journal of Environmental Science and Sustainable Development

The area of Kamojang Nature Tourism Park, which was originally only 255 hectares, has expanded to 2,391 hectares following a changes in the Nature Reserve area. This change has raised concerns and objections from various parties, as it is considered to open opportunities for exploitation, threaten biodiversity, and potentially trigger tourism activities that may degrade the environmental quality of the areaThis study aims to analyze the sustainability status index of the Kamojang Natural Tourism Park following the status downgrade from a Nature Reserve to a Natural Tourism Park. A descriptive qualitative approach used, utilizing the Multi-dimensional Scaling model to determine …

Bibliometric And Content Analysis: Application Of The Theory Of Planned Behavior In Environmental Issues, Nofi R.A.R Samputra, Kurniawaty Iskandar, Tri Edhi Budhi Soesilo, Dwi Nowo Martono

Journal of Environmental Science and Sustainable Development

Theory of Planned Behavior (TPB) has been widely applied in environmental science as it closely linked to human behavior. This study aims to analyze research trends, identify potential future research, and propose the development of an integrative TPB model to address gaps in previous research. A total of 120 articles were selected from the Scopus database using rigorous screening criteria, including title-based selection, English-language articles, and a manual abstract review, covering publications up to April 4, 2025. Bibliometrix, VOSviewer, and MindMup were used to support keyword visualization and content interpretation. The results show that China and the United States lead …

Microbial And Ecophysiological Indices Of Agricultural Soils As Affected By Different Farming Practices In Tropical Monsoon Climate, Gulshan Ara Begum, Sajal Roy, Nasrin Chowdhury

Journal of Environmental Science and Sustainable Development

Organic farming is increasingly promoted as a sustainable alternative to conventional agriculture due to its positive effects on soil microbial dynamics, a critical component of ecosystem functioning. However, empirical comparisons of microbial responses across these systems are scarce. Therefore, we conducted a study to assess the various microbial indices of soils taken from distinct crop fields (e.g., brinjal, chilli, tomato, bean, and fallow lands) managed under both conventional and organic farming techniques. Composite soil samples from surface layer (0–15 cm) were collected in a completely random way from Bandarban upazila of Bangladesh (22°11'24.0"N 92°09'00.0"E, organic fields; 22°10'12.0"N 92°09'36.0"E, conventional fields). …

Convergence In Action: Where Environmental Science Meets Sustainable Development Practice, Herdis Herdiansyah

Journal of Environmental Science and Sustainable Development

Since the introduction of the Sustainable Development Goals (SDGs), the pursuit of human well-being through development and planetary sustainability for a better future has become a global discourse (de Jong & Vijge, 2021; Naseer et al., 2025; Arora-Jonsson, 2023). The SDGs have catalyzed interdisciplinary dialogue and strengthened global commitment (Naseer et al., 2025) by emphasizing the balance and integration of economic, social, and environmental aspects (Sedovs et al., 2025). However, the assumption that all goals can be achieved simultaneously and harmoniously seems hasty and potentially ignores the inherent tensions between ecological and …

Resilient Greenport Conceptual Model: Integrating Masterplan With Flood And Land Subsidence Disaster Management, Teuku Khamil Syamsura, Kosuke Mizuno, Supriatna Supriatna

Journal of Environmental Science and Sustainable Development

Hydrometeorological disasters, such as flooding by seawater intrusion and land subsidence from excessive water resource usage, significantly disrupt maritime traffic and port infrastructure, leading to severe economic and social consequences. This study aims to develop a conceptual model for a disaster-resilient and environmentally friendly port. The primary objective is to analyze the components of the Port Masterplan (RIP), the Greenport concept, current operational conditions, and surrounding disaster threats for develop conceptual model. A mixed-methods approach, integrating quantitative and qualitative analyses, was employed. The study examined various components of the Tanjung Priok Port Masterplan, operational data, and environmental and disaster risk …

Understanding Offline Pro-Environmental Identity: Factors And Implications, Tusyanah Tusyanah, Rahayu Puji Haryanti, Wijang Sakitri, Ismiyati Ismiyati, Nur Chayati

Journal of Environmental Science and Sustainable Development

Given global environmental challenges, developing sustainable behavior among pre-service teachers is crucial, as they will be responsible to educate future generations who must contribute to solving environmental problems. Offline pro-environmental identity refers as the individual's environmental commitment in real-life setting. Previous studies have primarily focused on online identity and behavioral intentions, while real-life behavioral context have not been fully examined. The goal of this study was to investigate the effects of the online pro-environmental identity, the perceived behavioral control, and the moral identity on the behavioral intention and the offline pro-environmental behavior. The study employed a quantitative research design and …

Comparison Of Kinematic Outputs And Reliability Of Plug-In Gait Versus Conventional Gait Model 2 During Cycling, Erin Mccallister, Nicholas Russell

International Journal of Exercise Science

International Journal of Exercise Science 18(7): 712-726, 2025. Motion analysis is used to measure proper bicycle fit, avoid injury, and improve cycling performance. Small changes in position can impact joint kinematics and risk for overuse injury. Concerns regarding the often-used biomechanical model Plug-in-Gait (PIG) resulted in the creation of Conventional Gait Model 2 (CGM2). This study aims to compare kinematic outputs of these two models for cycling biomechanics plus between-day reliability of each model. Thirty-five participants participated in two experimental sessions. PiG and CGM2 marker sets were applied, and data was collected while cycling between 80-90 rpm. Model outputs were …

Record Of Michigan Mosquito Species (Diptera: Culicidae) Collected In A Natural Focus Of Jamestown Canyon Virus In 1984, Paul R. Grimstad, Michael J. Mandracchia

The Great Lakes Entomologist

Data are presented on the seasonal succession of 22 species of mosquitoes collected at the Houghton Lake Wildlife Research Area, Missaukee County, in northcentral Michigan during a three-month period in 1984. Nine species represent new county records.

Social Trends Emerging In A Mexican Village Community Involved In A Leaf Nutrient Supplementation Project Offered As Nutritional Intervention Scheme, T M. Ostrowski-Meissner, H T. Ostrowski-Meissner

IGC Proceedings (1977-2023)

Social analysis of the leaf nutrient supplementation (LNS) project using protein concentrate from alfalfa in a Mexican village (Saltillo) has been made in an attempt to identify the social trends associated with the community's involvement with protein extraction from green vegetation as a nutritional intervention scheme. Through preliminary on site observation and review of available scheme records, emergent social trends were identified in:

1. the voluntary diversification of nutritional habits,

2. an extension of health services for the community, and

3. subtle change within the village social milieu. The suitability of the LNS project is discussed, and areas for further …

Effects Of Fluidized Bed Combustion Residue Application To Reclaimed Mine Land On Yield And Composition Of Forage And Performance Of Grazing Steers, K O. Smedley, J P. Fontenot, V G. Allen, H D. Perry, O L. Bennett

IGC Proceedings (1977-2023)

Fluidized bed combustion residue (FBCR), a waste product resulting from the addition of limestone to coal prior to combustion, is high in sulfur (S) and has approximately 50% of the neutralizing capacity of limestone. Research was conducted to study effects of repeated applications of FBCR to pastures on forage yield and composition, and on performance and health of grazing cattle. Three treatments were applied in three replicated .81 ha pastures located on acidic reclaimed land which had been previously mined by mountain top removal. Treatments were control, dolomitic limestone (3380 kg/ha) and FBCR (6760 kg/ha), applied in split applications. Pastures …

Methodology For Environmental Toxicology: Assessing Feeding And Rearing Strategies In Largemouth Bass (Micropterus Nigricans) Larvae, Amber Hitchins, Hannah Mcneese, Deise Cruz, Andrew Todd, Maria Sepúlveda, Tyler Hoskins

Discovery Undergraduate Interdisciplinary Research Internship

Poly- and perfluoroalkyl substances (PFAS) are an anthropogenic group of chemicals known for their persistence in the environment and their potential to bioaccumulate. PFAS can bioaccumulate in largemouth bass (Micropterus nigricans), yet the effects of exposure on bass larvae are unknown. While largemouth bass are commonly raised for aquaculture, techniques for rearing bass which enable controlled experiments at known densities, a necessity for toxicity studies, are poorly understood. Furthermore, the live prey typically used in existing approaches are not suitable for PFAS studies due to high background concentrations. The objective of this project was to assess different venues …

Ctnna3 Deficiency Promotes Heart Regeneration By Enhancing Cardiomyocyte Proliferation In Neonatal Mice, Sha Zou, Wuhou Dai, Wufan Tao, Jifen Li, Zeyi Cheng, Hongyan Wang

Department of Medicine Faculty Papers

Background: Heart regeneration requires renewal of lost cardiomyocytes. However, the mammalian heart loses its proliferative capacity soon after birth, and the molecular signaling underlying the loss of cardiac proliferation postnatally is not fully understood.

Purpose: This study aimed to investigate the role of Catenin alpha 3 (Ctnna3), coding for alpha T catenin (αT-catenin) protein in regulating cardiomyocyte proliferation and heart regeneration during the neonatal period.

Methods: Here we report that ablation of Ctnna3 and highly expressed in hearts, accelerated heart regeneration following heart apex resection in neonatal mice.

Results: Our results show that Ctnna3 deficiency enhances cardiomyocyte proliferation …

How Beliefs Shape What We Consider When Choosing What To Eat, Christopher Gustafson

Cornhusker Economics

What determines which foods we consider? One factor, certainly, is what we like to eat, and if liking what we eat were the sole relevant outcome of food consumption, there would be little to say about incomplete consideration. However, diet quality has important, well-documented effects on health outcomes (as well as other important considerations, such as differential environmental impact). Close to 75 percent of American adults are overweight or obese, which increases the likelihood that the individual will experience one or more serious non-communicable diseases. A food’s healthiness is an important consideration for many people when making food choices, but, …

Cultivating The Wild: Strategies For Entrepreneurs Navigating Niche And Specialty Food Markets, Trey Malone, Courtney F. Cooper

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Introduction Consumer demand for authenticity and sustainability in food systems is growing, creating both opportunities and challenges for entrepreneurs in niche and specialty markets. While wild-foraged and hunted foods are often perceived as more "natural" and sustainable, attempts to scale these products through farming may compromise their perceived authenticity.Methods To explore these dynamics, we conducted discrete choice experiments with a nationally representative sample of 1,039 U.S. consumers. Participants evaluated their willingness to pay (WTP) for salmon, venison, and morel mushrooms, presented in both wild-foraged/hunted and farm-raised forms.Results Consumers exhibited strong preferences for wild versions of all three products, with average …

Retrotransposon Methylation Profiles And Survival In Black Women With High-Grade Serous Ovarian Carcinoma, Christelle Colin-Leitzinger, Katherine A Lawson-Michod, Courtney E Johnson, Irma M Vlasac, Sean Yoder, Tania Mesa, Dana Roeber, Chad Huff, Michelle A T Hildebrandt, Kristin Haller, Anthony J Alberg, Elisa V Bandera, Melissa Bondy, Michele L Cote, Theresa Hastert, Edward S Peters, Paul D Terry, Andrew B Lawson, Andrew Berchuck, Brooke L Fridley, Jing-Yi Chern, Jennifer A Doherty, Jeffrey R Marks, Joellen M Schildkraut, Brock C Christensen, Lucas A Salas, Lauren C Peres

Faculty, Staff and Student Publications

Introduction: Retrotransposons (REs) constitute nearly half of the genome and include long terminal repeat (LTR) elements, Long INterspersed Elements (LINE), and Short INterspersed Elements (SINE). REs are typically silenced in somatic tissues via DNA methylation but can be reactivated through DNA hypomethylation, potentially impacting gene regulation. Here, we investigate genome-scale profiles of RE methylation in high-grade serous ovarian carcinoma (HGSOC) and associations with survival among Black women.

Methods: Methylation levels of LTR, LINE-1, and Alu (type of SINE) in 200 HGSOC tumors were predicted using a random forest approach and clustered using multiple consensus algorithms. Associations between RE methylation clusters …

Health Patterns Of Dementia Caregivers With Chronic Pain: Latent Profile Analysis Of The Promis-29 Measure, Shelbie G. Turner, Dakota D. Witzel, Shana Garza, Karl Pillemer, M Carrington Reid

Human Genetics Publications

We identified patterns of health and functioning in a national sample of dementia family caregivers experiencing chronic pain (N = 273). Utilizing latent profile analysis of the 29-item Patient-Reported Outcomes Measurement Information System (PROMIS-29), we identified three discrete groups: an average group (48%), a low health challenges group (30%), and a high health challenges group (22%). Women were more likely to be in the high (vs. low) health challenges group. There are discrete health patterns of caregivers with chronic pain, with over 20% reporting comorbid poor health and functioning that may negatively affect caregiving quality. Screening caregivers for high …

Effects Of Aerobic Exercise On Physical Fitness In Obesity Using Fox Vs. Tanaka’S Maximum Heart Rate And Percentage Vs. Karvonen Methods, Maninthorn Rugbumrung, Tavarintorn Rukbumrung, Sittiwit Impanya, Adison Thurayot

International Journal of Exercise Science

International Journal of Exercise Science 18(8): 695-711, 2025. The intensity of aerobic exercise is influenced by maximum heart rate (MHR), which can be assessed through an incremental exercise test. However, this method requires specialized equipment and a level of fitness that individuals who are sedentary or overweight may lack. Therefore, estimating MHR using formulas is essential. Various methods exist to calculate MHR, but their effects on fitness in obese individuals remain unclear. This study aimed to investigate the effects of aerobic exercise using different intensity calculation methods, specifically comparing Fox's and Tanaka's maximum heart rate (MHR) equations, as well as …

Computational And In Vitro Investigation Of P. Crocatum Bioactive Compounds As Pancreatic Lipase Inhibitors, Gusnia Meilin Gholam, Dimas Andrianto, Dewi Anggraini Septaningsih, Mega Safithri

Karbala International Journal of Modern Science

Obesity, a prevalent metabolic disorder characterized by excessive fat accumulation, can severely affect overall health if left untreated. This study investigated the potential of a 70% ethanol extract from Piper crocatum (red betel) leaves as an in vitro inhibitor of pancreatic lipase (PL), supported by computational analyses to identify alternative compounds to orlistat. The phytochemical profile was characterized using LC-MS/MS, revealing alkaloids and terpenoids with contents of 1.1 ± 0.01 mg CE/g and 3.14 ± 0.3 mg UAE/g, respectively. The extract exhibited 49 ± 9.1% inhibition of PL activity. Molecular docking identified three promising compounds: calanolide A (10.43 kcal/mol), myricanone …

Cocoa Polyphenols Alter The Fecal Microbiome Without Mitigating Colitis In Mice Fed Healthy Or Western Basal Diets, Eliza C. Stewart, Mohammed F. Almatani, Marcus Hayden, Giovanni Rompato, Jeremy Case, Samuel Rice, Korry J. Hintze, Abby D. Benninghoff

Animal, Dairy, and Veterinary Science Student Research

Background/Objectives: Chronic inflammation and Western-style diets elevate colorectal cancer (CRC) risk, particularly in individuals with colitis, a feature of inflammatory bowel disease (IBD). Diets rich in polyphenol-containing functional foods, such as cocoa, may reduce gut inflammation and modulate the gut microbiome. This study investigated the impact of cocoa polyphenol (CP) supplementation on inflammation and microbiome composition in mice with colitis, fed either a healthy or Western diet, before, during, and after the onset of disease. We hypothesized that CPs would attenuate inflammation and promote distinct shifts in the microbiome, especially in the context of a Western diet. Methods: …

Non-Mutated Human Tau Stimulates Alzheimer’S Disease-Relevant Neurodegeneration In A Microglia-Dependent Manner, Ethan R Roy, Qiang Wang, Kexin Huang, Sanming Li, Yuanyuan Fan, Estrella Escobar, Constance L Atkins, Shuning Huang, Juan J Herrera, Wenbo Li, Clare Pridans, Xiaobo Zhou, Cynthia Ju, Wei Cao

Faculty, Staff and Student Publications

The accumulation of abnormal, non-mutated tau protein is a key pathological hallmark of Alzheimer's disease (AD). Despite its strong association with disease progression, the mechanisms by which tau drives neurodegeneration in the brain remain poorly understood. Here, we selectively expressed non-mutated or mutated human microtubule-associated protein tau (hMAPT) in neurons across the mouse brain and observed neurodegeneration in the hippocampus, especially associated with non-mutated human tau. Single-nuclei RNA sequencing confirmed a selective loss of hippocampal excitatory neurons by the wild-type tau and revealed the upregulation of neurodegeneration-related pathways in the affected populations. The accumulation of phosphorylated tau was accompanied by …

Glucose Metabolism And Its Direct Action In Cancer And Immune Regulation: Opportunities And Challenges For Metabolic Targeting, Bo-Syong Pan, Che-Chia Hsu, Hsin-En Wu, Yuan-Ru Chen, Xiaobo Zhou, Shu-Chi Wang, Chia-Yang Li, Hui-Kuan Lin

Faculty, Staff and Student Publications

Glucose metabolism is a pivotal hub for cellular energy production and the generation of building blocks that support cell growth, survival, and differentiation. Cancer cells undergo metabolic reprogramming to sustain rapid proliferation, survive in harsh microenvironments, and resist therapies. Beyond producing energy and building blocks to meet cancer cell demands, glucose metabolism generates numerous metabolites that serve as signaling molecules, orchestrating signaling pathways and epigenetic modifications that regulate cancer cell phenotypes and immunity. In this review, we discuss how glucose, through its metabolism and direct actions, influences diverse biological processes driving cancer progression and therapeutic resistance, while also exploring metabolic …

In Silico Prediction Of Cytotoxic T-Cell Epitopes From Helicobacter Pylori Virulence Factors Using An Immunoinformatics Approach, Demy Valerie Chacon, Kiana Alika Co, Daphne Noreen Enriquez, Aubrey Love Labarda, Reanne Eden Manongsong, Edward Kevin B. Bragais

Biology Faculty Publications

Background: Helicobacter pylori infects approximately half of the global population, leading to gastric and duodenal ulcers. Despite the availability of antibiotics, challenges such as patient reluctance, high treatment costs, and antibiotic resistance limit their effectiveness, making vaccination a promising alternative. This study used immunoinformatics to identify candidate epitopes for a multiepitope vaccine construct against H. pylori.

Material and methods: The protein variability server was utilized for conservation analysis. The epitopes were screened for antigenicity, allergenicity, toxicity, cross-reactivity, and population coverage. Selected epitopes were docked with their corresponding human leukocyte antigen (HLA) alleles, and thermodynamic quantities were determined. Five virulence …

Resilience Mediates The Association Between Discrimination And Psychological Distress Among Rural, Black Women In South Carolina: A Structural Equation Model, Camryn Garrett, Abhisheak Aggrawal, Huiyi Xia, Cheuk Chi Tam, Bankole Olatosi Ph.D., Sarah Wilcox Phd, Xiaoming Li Ph.D., Shan Qiao Ph.D.

Faculty Publications

Introduction

The marginalization of intersecting racial, gender, and rural identities compounds the structural vulnerabilities (e.g., discrimination, stigma, health disparities) experienced by Black women in South Carolina’s rural areas. Discriminatory experiences can deteriorate mental health; however, resilience can potentially reduce the ill effects of discrimination by decreasing associated psychological distress. This study examines the mediating role of resilience in the association between discrimination and psychological distress among rural, Black women in South Carolina in the post-pandemic era.

Methods

Black women (n = 300, mean age = 48.6) living in rural South Carolina were recruited from January to February 2024 using …

Ecoimmunology Of Dirty Physella Acuta Snails, Kevin Arthur Mcquirk

Biology ETDs

Research on Physella acuta, an invasive freshwater snail, reveals insights into its parasitology, fitness, and molecular biology. Morphological and molecular analyses revealed two distinct mitogenomic lineages, local (B) and invasive (A) strains of native P. acuta, both having comparable trematode infection rates, suggesting that the low infection rates observed from A in invaded regions result from "enemy release" rather than immune incompatibility. Fitness assessments showed that while lineages A and B performed similarly under laboratory conditions, rewilding experiments revealed significantly higher fitness in lineage A. Transcriptomic profiling supported these differences, with A snails showing greater immune-related gene expression and …

Using Triple Oxygen Isotopes To Investigate Water Usage In Desert Ecosystems, Cloe V. Knutson

Earth and Planetary Sciences ETDs

In arid environments, maintaining water balance can be more critical for survival than obtaining food. As droughts intensify, understanding how animals manage body water is essential. Triple oxygen isotope analysis provides a novel way to trace water sources in animals, distinguishing contributions from free water, food-derived oxygen, and atmospheric O₂. We developed an equilibration method using CO₂–H₂O oxygen exchange and measured δ^’18O and Δ^’17O values via tunable infrared laser direct spectroscopy (TILDAS). Validated against traditional fluorination methods, this technique enables streamlined analysis of whole blood and plant samples. Applied to …

Addressing Head Injuries In High School Football: An Assessment Of Best Practices And Challenges In The Area Of Concussions As Held By New Mexico Activities Association High School Varsity Football Head Coaches And Athletic Directors, Alonzo Maestas

Health, Exercise, and Sports Sciences ETDs

Youth sports participation in the United States offers children and adolescents opportunities for physical activity, skill development, social interaction, and personal achievement. However, with sports comes the risk of injury, including concussions. Concussions can have serious long-term consequences such as chronic traumatic encephalopathy (CTE). In response to the risks of concussions, New Mexico enacted a concussion law, NM Stat 22-13-31. In addition to this law, the New Mexico Activities Association (NMAA) and National Federation of High School Associations (NFHS) have also given guidance on how to manage concussions.

However, there is a gap in the literature concerning concussion education, best …

Gallic, Aconitic, And Crocetin Acids As Potential Tnf Modulators: An Integrated Study Combining Molecular Docking, Dynamics Simulations, Admet Profiling, And Gene Expression Analysis, Adolat Manakbayeva, Ahdrey P. Bogoyavlenskiy, Timur Kerimov, Igor Yershov, Pavel Alexyuk, Madina Alexyuk, Vladimir Berezin, Vyacheslav Dushenkov

Publications and Research

Organic acids, as natural metabolites, play crucial roles in human metabolism and health. Tumor Necrosis Factor (TNF), a pivotal mediator in immune regulation and inflammation, is a key therapeutic target. We evaluated ten organic acids as TNF modulators using in silico molecular docking, followed by detailed ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) profiling and molecular dynamics (MD) simulations for three lead candidates: gallic, aconitic, and crocetin acids. Their effects on TNF gene expression were then assessed in vivo using a mouse leukocyte model. The in silico results indicated that crocetin had the highest TNF binding affinity (−5.6 to −4.6 …

Exploration, Expansion, And Optimization Of Bacteriophage Virus-Like Particle Vaccines, Andzoa N. Jamus

Biomedical Sciences ETDs

Bacteriophage virus-like particles are highly immunogenic, self-assembling platforms used for various vaccines. This work shows efforts to explore new vaccine candidates against Chlamydia trachomatis, targeting proteins that play critical roles in attachment and entry into the host cell, as well as in pathogenesis and bacteria proliferation. This work also shows the optimization of previously identified candidates, by investigating how altering routes of immunization can change the antibody profiles elicited, both systemically and locally. Finally, this work shows the establishment of various Chlamydia infection models in an effort to expand the ways in which vaccine efficacy can be investigated, particularly …

Immune Regulation In Fungal Airway Inflammation And Host Defense, Ruoning Wang

Biomedical Sciences ETDs

Asthma is a chronic inflammatory disease of the lungs characterized by complex pathophysiological mechanisms and diverse endotypes, primarily categorized as T helper type 2 cell (Th2)-high and Th2-low asthma. Despite significant advances in understanding asthma pathogenesis, the mechanisms governing the pulmonary immune system’s response to external challenges have not been fully understood. To address these gaps, this comprehensive project was designed to investigate the interplay between fungal infections and specific CD4⁺ T cell populations in asthma. Our findings suggest that fungal infections can contribute to neutrophilic asthma by promoting the migration of platelet-myeloid aggregates into the lungs. We also …

Elevated Nr2f1 Underlies The Persistence Of Invasive Disease After Treatment Of Braf-Mutant Melanoma, Manoela Tiago, Timothy J. Purwin, Casey D. Stefanski, Renaira Oliveira Da Silva, Mitchell E. Fane, Yash Chhabra, Jelan I. Haj, Jessica L. F. Teh, Rama Kadamb, Weijia Cai, Sheera Rosenbaum, Vivian Chua, Nir Hacohen, Michael A. Davies, Jessie Villanueva, Inna Chervoneva, Ashani T. Weeraratna, Dan A. Erkes, Claudia Capparelli, Julio A. Aguirre-Ghiso, Andrew E. Aplin

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Despite the success of targeted inhibitors in cutaneous melanoma, therapeutic responses are limited by the aged tumor microenvironment and drug-tolerant residual cells. Given the similarities between drug tolerance and cellular dormancy, we studied the dormancy marker, nuclear receptor subfamily 2 group F member 1 (NR2F1), in response to BRAF-V600E inhibitors (BRAFi) plus MEK inhibitors (MEKi) in BRAF-mutant melanoma models. Transcriptomic analysis of melanoma patient samples treated with BRAFi + MEKi showed increased NR2F1. NR2F1 was highly expressed in the drug-tolerant invasive cell state of minimal residual disease in patient-derived and mouse-derived xenografts on BRAFi + MEKi. NR2F1 over-expression was sufficient …