Digital Commons Network^™

Design And Application Of A Tau Seed Amplification Assay For Screening Inhibitors Of Tau Seeding, Damian Gorski, Haley Evans, Tyler Allison, Carla Barria, Danielle Harrison, Victor Banerjee, Nicolas Mendez, Mohammad Shahnawaz, Sanne Kaalund, Jonas Folke, Susana Aznar, Paul Schultz, Fei Wang, Claudio Soto

Faculty, Staff and Student Publications

Background: Tau protein aggregates are a key pathological hallmark of Alzheimer's disease (AD) and are closely associated with cognitive decline and neurodegeneration. It is proposed that tau aggregates faithfully propagate throughout the brain by self-templating their disease-associated conformation onto natively-folded tau monomers, thereby inducing their aggregation and incorporation into growing fibrils. As such, the inhibition or modulation of tau seeding and aggregation represents a viable therapeutic strategy for AD and other tauopathies.

Methods: We have recently developed seed amplification assays (SAA) for the detection and amplification of small quantities of misfolded protein aggregates in various neurodegenerative diseases. In this article, …

Public Perceptions Of Universities As Public Entities During The Covid-19 Pandemic Era, Jinho Jung, Laura Barrett, Nicole Olynk Widmar, Mario Ortez

Department of Agricultural Economics Faculty Publications

Higher education institutions are facing challenges in navigating social, cultural, and political issues. The online presence of higher education presents a new set of opportunities and challenges; knowledge, news, and continuing education are increasingly disseminated online. Additionally, the public, student bodies and stakeholders are increasingly looking at reviews or seeking opinions via online and social media to form opinions on institutions. This analysis uses online and social media to analyze and compare perceptions of two public universities in Indiana, namely Purdue University and Indiana University. These two Indiana institutions are located in the same state, allowing for comparisons of public …

Decorin Evokes A Pro-Lysosomal Pathway In Lymphatic Endothelial Cells, Gabriel J. Pascal, Dipon K. Mondal, Sadie Kim, Christopher Xie, Devanarayanan Siva Sankar, Jörn Dengjel, Renato V. Iozzo

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The lymphatic system is critical to the body's immune and circulatory system, and lymphangiogenesis, the development of new lymphatic vessels from pre-existing ones, is a significant process capitalized upon by cancer during tumorigenesis. Decorin is a small leucine-rich proteoglycan which we have previously shown to be anti-tumorigenic and a suppressor of lymphangiogenesis. We have also shown that decorin exercises its anticancer properties through its ability to evoke autophagy. Through a comprehensive and unbiased proteomic analysis, we explored the implications of decorin exposure on protein expression within mouse lymphatic endothelial cells. We discovered that decorin enriches several protein pathways, notably proteasomal …

Observational Activation Of Anterior Cingulate Cortical Neurons Coordinates Hippocampal Replay In Social Learning, Xiang Mou, Daoyun Ji

Faculty, Staff and Students Publications

Social learning enables a subject to make decisions by observing the actions of another. How neural circuits acquire relevant information during observation to guide subsequent behavior is unknown. Utilizing an observational spatial working memory task, we show that neurons in the rat anterior cingulate cortex (ACC) associated with spatial trajectories during self-running in a maze are reactivated when observing another rat running the same maze. The observation-induced ACC activities are reduced in error trials and are correlated with activities of hippocampal place cells representing the same trajectories. The ACC activities during observation also predict subsequent hippocampal place cell activities during …

09.29.2025 Ored Connect, Liz Williamson

ORED Newsletter

• Contest for Biosafety and Biosecurity Month
• ORED Website Walk Through training
• MIG Applications
• Field Fest

Uncovering The Pml::Rara Fusion In Cytogenetically Cryptic And Fish-Negative Acute Promyelocytic Leukemia-A Case Report And Comprehensive Literature Review, Busra Delikkaya, Jaime Eberle-Singh, Arianna Morton, Jerald Gong, Jinglan Liu

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The PML::RARA fusion resulting from t(15;17) is the genetic hallmark of acute promyelocytic leukemia (APL), typically detected by cytogenetics and/or fluorescence in situ hybridization (FISH) studies. Rarely, APL patients present with normal cytogenetics and FISH findings, complicating diagnosis and delaying life-saving therapy. We report a 23-year-old male with clinical, morphologic and immunophenotypic features consistent with APL but negative for FISH studies. Despite prompt initiation of all-trans retinoic acid (ATRA) based on clinical suspicion, the patient succumbed to intracranial hemorrhage. Quantitative reverse transcriptase PCR (qRT-PCR) confirmed a long isoform PML::RARA fusion. A review of 34 published cytogenetics- and FISH-negative cases since …

Current States In Understanding Oligodendroglia-Mediated Neurological Issues In Neurofibromatosis Type 1 (Nf1), Benjamin E Aghoghovwia, Cheng-En Shen, Sabiha Bano, Nandini Shyamala, Alesandra Echeandia Marrero, Khushboo Irshad, Samer Sharafaldin, Nicole M Brossier, Yuan Pan

Faculty, Staff and Student Publications

Neurofibromatosis type 1 (NF1) is among the most common neurogenetic disorders and is associated with an increased risk of developing tumors in the nervous system. Additionally, up to 80% of patients with NF1 experience neurological complications, including deficits in attention, memory, and executive function. Significant effort has been dedicated to studying how NF1 mutations autonomously dysregulate neuronal function. Increasing evidence indicates that NF1 mutations also dysregulate the oligodendroglial lineage that contributes to neurological issues in NF1. Here, we summarize our current understanding of how NF1 mutations impact the oligodendroglial lineage homeostasis and plasticity. We also discuss gaps in knowledge, potential …

Structural Variation Of The Right Coronary Ostium And Its Implications To Cardiac Medicine, Jessica L. Hensley, Joseph X. Anders, Timothy Robinson, William Srinivasan, Donald G. Matz, Ethan L. Snow

The Journal of Integrative Physiology

Introduction: The high oxygen demand of the heart’s myocardium is met via perfusion through the coronary arterial system. During embryological development, the right (RCA) and left (LMCA) coronary arteries fuse onto the aortic root, creating right (RCO) and left (LCO) ostial openings respectively to join the closed circulatory system. Regardless of their widely variable branching patterns, the ability of the RCA and LMCA to perfuse the myocardium initially relies on the functional patency of the coronary ostia. The objective of this study is to examine a unique extra-ostial tissue structure found overlying an RCO and provide a literature-based discussion of …

The Evaluation Of Variants Within Eight Collagen Genes Col1a1, Col1a2, Col3a1, Col5a1, Col11a1, Col15a1, Col22a1, And Col27a1 And A Risk Of Non-Contact Knee Ligament Rupture In Elite Handball Players: A Case-Control Study, Katarzyna Krawczak-Wójcik, Andrzej Mastalerz, Aleksandra Bojarczuk, Monika Johne, Alison V. September, Aleksandra Garbacz, Katarzyna Komar, Ewelina Maculewicz

Baltic Journal of Health and Physical Activity

Introduction: Knee ligament ruptures are common injuries among athletes. While most previous studies examined single collagen gene polymorphisms, few have investigated haplotype-level associations across multiple genes, particularly in elite handball players. Materials and methods: This case-control study analyzed variants in eight collagen genes (COL1A1, COL1A2, COL3A1, COL5A1, COL11A1, COL15A1, COL22A1, and COL27A1) in 103 elite Polish handball players with non-contact knee ligament rupture and 226 matched controls. Results: The COL22A1 rs11784270 CC genotype was associated with an increased risk (OR = 2.88, p = 0.01), with the strongest effect in the …

Scaling Symmetry Of The Hodgkin-Huxley Mathematical Model, Vladimir Valerievich Galanin

Progress in Scale Modeling, an International Journal

A computational method based on the application of a scaling transformation to the Hodgkin-Huxley mathematical model of the biological neuron and its generalizations to the case of various neural systems is proposed. The method makes it possible: 1) to obtain invariants of the scaling transformation of the Hodgkin-Huxley model; 2) to find self-similarity regions corresponding to the modes of neural activity; 3) to determine the conditions for the similarity of models of various types of biological neurons; 4) to reduce the order of the system of differential equations of the Hodgkin-Huxley model for neural systems with synchronous activity. The capabilities …

Comparative Transcriptomics Reveals Novel And Differential Circular Rna Responses Underlying Interferon-Mediated Antiviral Regulation In Porcine Alveolar Macrophages, Jiuyi Li, Oluwaseun Adeyemi, Laura C. Miller, Yongming Sang

Food and Animal Sciences Faculty Research

Porcine Reproductive and Respiratory Syndrome (PRRS) causes significant economic losses in the swine industry. Circular RNAs (circRNAs), a class of stable non-coding RNAs, are increasingly recognized as regulators in immune responses and host–virus interactions. This study investigated the genome-wide circRNA responses in porcine alveolar macrophages (PAMs), key cell targets of PRRSV, following treatment with a modified live virus (MLV) vaccine or two interferon (IFN) subtypes (IFN-α1, IFN-ω5). Using RNA sequencing, we identified over 1000 differentially expressed circRNAs across treatment groups, revealing both conserved and distinct expression profiles. Gene Ontology and KEGG pathway analyses indicated that circRNA-associated genes are significantly enriched …

Early-Life Exposure To Organic Chemical Pollutants As Assessed In Primary Teeth And Cardiometabolic Risk In Mexican American Children: A Pilot Study, Vidya S. Farook, Feroz Akhtar, Rector Arya, Alice Yau, Srinivas Mummidi, Juan Lopez Alvarenga, Alvaro Diaz-Badillo, Roy G. Resendez, John Blangero

Human Genetics Publications

Early-life exposure to organic chemicals (OCs) may influence childhood obesity and associated cardiometabolic risk. These conditions have been shown to disproportionately affect minority populations such as Mexican Americans (MAs). However, information on the impact of organic chemicals on cardiometabolic risk in MA children is limited. Therefore, we conducted a pilot study to assess the extent to which exposure to organic chemicals influences cardiometabolic traits (CMTs) in MA children. We recalled 25 children from a previous study and collected 25 primary teeth from them. Chemical analyses of the teeth were performed using established protocols. Target analytes included acetaminophen (APAP); 3,5,6-trichloro-2-pyridinol (TCPy), …

Genomic Prediction And Association Mapping Of Early Season Flood Tolerance In Soybean, Caio Canella Vieira, Chengjun Wu, Derrick Harrison, Rafael Marmo, Liliana Florez-Palacios, Andrea Acuna, Daniel Rogers, Samuel B. Fernandes, Igor Fernandes, Grover Shannon, Heng Ye, Henry T. Nguyen

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Flooding has become a major threat to soybean [Glycine max (L.) Merr.] production as the frequency and intensity of extreme precipitations have been increasing due to climate change. While advances have been made in identifying soybean genetic resources and genomic regions associated with mid-season flood tolerance, there is limited understanding of early season flood tolerance at the vegetative growth stages V2/V4. This study aimed to identify genomic regions associated with early season flood tolerance using a diverse panel of 254 soybean accessions, as well as investigate the viability of implementing genomic prediction models for flood tolerance. Field trials were conducted …

A Multifaceted Analysis Of The Genetic Origins, Growth, And Invasive Potential Of Introduced Floridian Sugarcane, Indy Hughes

Student Theses and Dissertations

Saccharum spontaneum L., wild sugarcane, is an aggressive invasive species, listed by the USDA as a Federal Noxious Weed (FNW). Despite this, S. spontaneum is used to create hardy, pest-resistant cultivars of domestic sugarcane. All cultivars have a small percentage of wild parentage, but the dosage is low to lessen the chance of inheriting undesirable traits. Saccharum spontaneum’s naturalized presence in Florida has been reported since 1993 and likely escaped from regulated sugarcane breeding facilities. The proximity of S. spontaneum populations to modern cultivars, grown intensively in the state, could potentially increase the risk of invasion. It is imperative to …

Unraveling Crop Nitrogen-Water Dynamics With Hyperspectral-Thermal Sensing In Northern Central Valley, California, Shahryar Fazli, Surendra Maharjan, Wenzhao Li, Joshua B. Fisher, Rejoice Thomas, Fernando Romero Galvan, Gabriela Shirkey, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Ensuring global food security in the face of climate change requires optimizing crop water use and nutrient management. This study investigates the relationship between canopy nitrogen (N) and evapotranspiration (ET) across sunflower, rice, walnut, alfalfa, and plum crops using advanced remote sensing technologies. High-resolution hyperspectral data from NASAs Earth Surface Mineral Dust Source Investigation (EMIT) and thermal multispectral data from the Landsat-based OpenET system were analyzed over 1,135 km² in California. Regression analysis revealed strong spatial association between canopy N and ET for sunflower (R² = 0.82), rice (R² = 0.71), and walnut (R² = 0.68), …

Association Between Therapeutic Alliance And Clinical Outcomes In Virtual Telepsychiatry: A Retrospective Analysis Of Data From Talkiatry, Cheryl Person, Nathaniel David Phillips, Kartik Venkatachalam, Georgia Gaveras

Faculty, Staff and Student Publications

Background: Given the increasing demand for accessible mental health services, fully virtual telepsychiatry has become a vital component of modern health care delivery. Therapeutic alliance, the collaborative and affective bond between patients and therapists, is a well-established predictor of clinical outcomes in traditional face-to-face and teletherapy. However, the relationship between therapeutic alliance and clinical outcomes in an outpatient telepsychiatry setting remains less understood.

Objective: Our primary objective was to evaluate the relationship between therapeutic alliance and clinical outcomes for depression and anxiety in an outpatient telepsychiatry practice.

Methods: This retrospective study analyzed data from treatment-seeking adults receiving services from Talkiatry, …

Aging In Mice Alters Regionally Enriched Striatal Astrocytes, Kay E Linker, Violeta Duran-Laforet, Matthias Ollivier, Xinzhu Yu, Dorothy P Schafer, Baljit S Khakh

Faculty, Staff and Student Publications

Aging affects multiple organs and within the brain drives distinct molecular changes across different cell types. The striatum encodes motor behaviors that decline with age, but our understanding of how cells within the striatum change remains incomplete. Using single-cell RNA sequencing from young and aged mice we identify molecularly distinct astrocyte subtypes. We show that astrocytes change significantly with age, exhibiting downregulation of genes, reduced diversity, and a shift to more homogenous inflammatory transcriptomic profiles. By exploring where striatal astrocyte subtypes are located with single-cell resolution, we map astrocytes enriched in dorsal, medial, and ventral striatum. Age increases inflammatory marker …

Comparison Of Imaging Based Single-Cell Resolution Spatial Transcriptomics Profiling Platforms Using Formalin-Fixed Paraffin-Embedded Tumor Samples, Nejla Ozirmak Lermi, Max Molina Ayala, Sharia Hernandez, Wei Lu, Khaja Khan, Alejandra Serrano, Idania Lubo, Leticia Hamana, Katarzyna Tomczak, Sean Barnes, Jinzhuang Dou, Qingnan Liang, Rti Team, Maria Gabriela Raso, Ximing Tang, Mei Jiang, Beatriz Sanchez-Espiridion, Annikka Weissferdt, John Heymach, Jianjun Zhang, Boris Sepesi, Tina Cascone, Anne Tsao, Mehmet Altan, Reza Mehran, Don Gibbons, Ignacio Wistuba, Cara Haymaker, Ken Chen, Luisa M Solis Soto

Faculty, Staff and Student Publications

Imaging-based spatial transcriptomics (ST) is evolving as a pivotal technology in studying tumor biology and associated microenvironments. However, the strengths of the commercially available ST platforms in studying spatial biology have not been systematically evaluated using rigorously controlled experiments. We use serial 5 μm sections of formalin-fixed, paraffin-embedded surgically resected lung adenocarcinoma and pleural mesothelioma samples in tissue microarrays to compare the performance of the ST platforms (CosMx, MERFISH, and Xenium (uni/multi-modal)) in reference to bulk RNA sequencing, multiplex immunofluorescence, GeoMx, and hematoxylin and eosin staining data. In addition to an objective assessment of automatic cell segmentation and phenotyping, we …

A Novel Self-Inhaled Nicotine Exposure System In Murine Models Reveals Age-Dependent Metabolic Effects Of Nicotine, Xu Xu, Hailan Liu, Meng Yu, Qingzhuo Liu, Yongxiang Li, Yue Deng, Yuxue Yang, Jingjing Cheng, Yutian Liu, Mengjie Wang, Xing Fang, Longlong Tu, Olivia Z Ginnard, Kristine M Mcdermott, Darah Threat, Fuhui Wang, Meixin Sun, Xinming Liu, Jiamin Qiu, Xi Wu, Jinjing Jian, Tong Zhou, Megan E Burt, Sanika V Jossy, Junying Han, Chunmei Wang, Yongjie Yang, Yong Xu, Yuhan Shi

Faculty, Staff and Students Publications

Background: Nicotine is known to influence metabolism and body weight, yet existing murine models of nicotine exposure often fail to replicate human smoking behaviors accurately. To address this gap, we designed a cost-effective, self-administered nicotine inhalation system that allows mice to voluntarily inhale nicotine vapor generated from e-liquid.

Results: Using this system, we investigated the metabolic effects of nicotine exposure in adult and adolescent mice. Adult mice exposed to nicotine vapor for 10 days exhibited significant weight loss, which was rapidly regained upon cessation, paralleling human smoking patterns. In contrast, adolescent mice did not lose weight during nicotine exposure but …

Synthesis And Characterization Of Fe-Zn Bimetallic Nanoparticles Via Two-Step Laser Ablation And Their Antibacterial Activity, Hudhaifa M. Mohammed, Sahar Naji Rashid

Karbala International Journal of Modern Science

In this study, Nd: YAG laser at a wavelength of (1064 nm), energies of (300 and 400 mJ), and a pulse repetition rate of (3 Hz) was used to synthesize metallic nanoparticles from iron and zinc individually using a one-step pulsed laser ablation approach, followed by the two-step synthesis of bimetallic nanoparticles. The physical properties of the synthesized nanoparticles were then investigated using UV-visible (UV-Vis), X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), and energy-dispersive X-ray (EDX) techniques. The results of characterization of the obtained NPs confirmed the formation of core-shell nanocomposites, as evidenced by the increased absorbance intensity of …

The Path To A Green Transition: Just And Inclusive, Nour Elden Tarek Anwr Abd Elshafy

COP30

This paper explores the opportunities and challenges in achieving Just Transition in the agricultural sector, focusing on the critical areas of Climate investments in the field of training and public awareness, policy formulation, and promoting Inclusive sustainable livelihoods for the most affected groups, namely people with disabilities. It highlights the current gaps in Climate investments, the need for inclusive and effective policies, and the importance of ways to achieve just integration to reduce Poverty and unemployment rates. The paper proposes actionable solutions to align financial investments, policy frameworks, and Comprehensive practices with the Paris Agreement goals and Glasgow talks (COP26), …

Er Stress Tolerance Is Regulated By Copper-Dependent Perk Kinase Activity., Sarah E. Bond Newton, Xinglong Shi, Noah R. Beratan, Julia Perhacs, Jitendra K. Arya, Margaret K. Bond, Tali Gidalevitz, Cagla Akay-Espinoza, Donita C. Brady, Kelly L. Jordan-Sciutto

Farber Institute for Neuroscience Faculty Papers

Pancreatic/PKR-like endoplasmic reticulum (ER) kinase (PERK) is a kinase that, in response to ER stress, mediates dual homeostatic and pro-apoptotic signaling. Thus, intricate regulation is required for physiological function. Attempts to modulate PERK activity have shown that the determinants of adaptive vs. maladaptive signaling remain ambiguous. Here, with purified protein, we provide evidence that PERK binds copper, identifies residues required for interaction, and demonstrates that copper is necessary for kinase activity. Furthermore, cellular PERK activity can be modulated via copper availability, and this regulatory relationship can be manipulated to dictate ER stress tolerance. Critically, these phenomena translate to phenotypes in …

Characteristics And Potential Indigenous Bacteria Isolated From Shallot Farming In Brebes Regency As Diazinon Pesticide Remediation Agents, Wahyu Purbalisa, Dian Hendrayanti, Wahida Annisa Yusuf

Makara Journal of Science

Diazinon is an organophosphate pesticide that is widely used by shallot farmers in Brebes Regency. Due to its toxicity, diazinon residue in agricultural lands as well as plants’ cultivation is harmful to humans and the environment. Using indigenous soil bacteria as a remediation agent is an alternative solution to overcome diazinon contamination. Firstly, this study aimed to characterize isolates from diazinon-contaminated shallot farming. The second objective was to test the diazinon tolerance of the isolates in various diazinon concentrations. Isolation was carried out using nutrient agar (NA) media containing diazinon concentrations of 5, 25, 50, and 100 ppm. The characterization …

Sequence And Phylogenetic Analysis Of Citrus Maxima (Burm.) Merr. From Tomini Bay, Sulawesi Island, Based On The Maturase K Gene, Brenda Febrina Zusriadi, Novri Youla Kandowangko, Febriyanti Febriyanti

Makara Journal of Science

This study was conducted in the coastal area of Tomini Bay, Sulawesi Island, and it focused on Citrus maxima, a plant known for its unique fruit flesh colors, which range from yellow to pink, and its varying leaf stalk wings. The study aimed to analyze the variations in maturase K (matK) sequences, molecular characteristics, and phylogenetic relationships of two C. maxima samples from Tomini Bay compared to other C. maxima and Citrus species using data available in GenBank. The study utilized DNA barcoding with matK molecular markers, followed by phylogenetic tree construction using the maximum likelihood method …

Detection Method For Escherichia Coli Using Real-Time Polymerase Chain Reaction Targeting The Yhav Gene, Muktiningsih Nurjayadi, Anisa Fitriyanti, Royna Rahma Musie, Gusti Angieta Putri, Puan Aqila Azizah, Helzi Angelina, Grace Grace, Ananda Indah Putri Sihombing, Agus Setiawan, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Ayu Berkahingrum, Irma Ratna Kartika, Fera Kurniadewi, Vira Saamia, Shyi-Tien Chen, Bassam Aboemolak, Hesham Ali El Enshasy

Makara Journal of Science

Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed …

Employing Seca Recombinant Protein To Generate Polyclonal Antibodies For The Rapid Identification Of Phytoplasma, Arik Arubil Fatinah, Muhaimin Rifa'i, Estri Laras Arumingtyas, Sri Widyarti

Makara Journal of Science

Phytoplasma, a plant pathogen that threatens plant health, can induce diseases such as yellow wilt, virescence, phyllody, and witches’ broom. Although early detection methods using polyclonal and monoclonal antibodies have been developed, synthetic peptides offer advantages such as higher titers, predictable antigenic characteristics and superior sensitivity and specificity. Epitope mapping plays a key role in designing peptide antigens and includes gene synthesis, transformation, expression assessment, large-scale production, and purification. A study using New Zealand male White rabbits was conducted to generate antibodies against phytoplasma. The SecA (395–470) sequence, obtained from the preprotein translocase meets the criteria of being non-homologous, nonallergic, …

Gama Melon Parfum, A Potential Melon Genotype Tolerant Of Salinity Stress, Giri Nugroho, Tatik Chikmawati, Dian Sartika, Adib Fakhruddin Yusuf, Karso Suryo Putro, Vida Rahma Latifah, Budi Setiadi Daryono, Anjar Tri Wibowo, Awik Puji Dyah Nurhayati, Karlia Meitha, Miftahudin Miftahudin

Makara Journal of Science

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week …

Design And Validation Of The Telemedicine Comfort Tool, Stephanie Amador

Doctoral Dissertations and Projects

In recent years, telemedicine has become a valuable resource to improve communication between patients and health care providers. There are many benefits to telemedicine, but they are only realized if patients and healthcare providers can establish a comforting and trusting relationship between them. Although Telemedicine has positively impacted health care, it is only successful if patients and health care providers can communicate effectively in the virtual environment. This quantitative study developed and validated the Telemedicine Comfort Tool among members of Veterans on Deck. The Telemedicine Comfort Tool is a survey instrument with the goal of measuring the comfort level of …

Untangling The Roles Of Pulmonary Vagal Afferent Subsets With Inhaled Irritants, Teresa Darcey

USF Tampa Graduate Theses and Dissertations

The sensory nerves in the airways directly regulate critical defenses and indirectly regulate vital gas exchange. Airway function is largely controlled by the autonomic nervous system, and our understanding of its sensory regulation is directly connected to our ability to treat respiratory and cardiovascular pathologies. Airway sensory afferents are heterogeneous with respect to their electrophysiological properties, peptidergic capacity, and stimuli sensitivity, which at least in part dictate some of their various functions. There is evidence that embryological origin impacts reflex function: activation of the neural crest-derived population of sensory afferents has been shown to evoke the cough reflex whereas activation …

Bridging The Gap: Does Individual Crossfit Open Performance & Event-Type Influence Crossfit Games Team Performance?, Anthony J. Mack

Doctoral Dissertations and Projects

Individual CrossFit Open (CFO) and CrossFit Games (CFG) performance factors, including physiological metrics, sex, competitive experience, and workout pacing strategies, are well established; however, there is a gap in the literature comparing CFO and CFG team performance. The purpose of this study was to examine whether individual CFO scores and event category (i.e., monostructural, gymnastics-skill, weightlifting, or work capacity) influence a team's final score at the CFG. Teams (n = 196) from the 2018-2024 CFG seasons were included in data collection. Data from CFO workouts (n = 28) and CFG team events (n = 62) were extracted from games.crossfit.com. Scores …