Digital Commons Network^™

Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing

Food and Machinery

[Objective] To establish a rapid detection method for viable Salmonella based on specific phage infection and recombinase polymerase amplification (RPA) in response to the key challenge that traditional molecular biological detection methods have difficulty in conveniently distinguishing between viable and non-viable foodborne pathogens. [Methods] Taking advantage of the characteristic that O-1 phage only proliferates in viable bacteria, the phage is added to the enriched sample. After further incubation, total DNA is extracted. Fluorescent RPA is used to determine whether the phage has significantly proliferated, so as to judge the presence of viable Salmonella in the original sample. [Results] After optimization, …

Establishment Of An Indirect Competitive Elisa For Sulfadimethoxine, Han Lu, Huang Mingyue, Yu Xiang, Li Sijia, Cao Jinbo, Wang Yao, Kang Huaibin

Food and Machinery

[Objective] To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of sulfadimethoxine (SDM) in animal-derived foods. [Methods] An artificial antigen is synthesized by conjugating SDM with carrier proteins by the diazotization method. A highly sensitive antibody against SDM is prepared by immunizing animals. Based on this, an ic-ELISA is established and the detection system is optimized. Its detection performance is characterized, and spiked recovery tests are carried out. [Results] When the concentration of the coating antigen is 0.500 μg/mL, the dilution ratio of the horseradish peroxidase-labeled goat anti-rabbit secondary antibody is 1:5000, the reaction time of the …

Migration Volume Determination And Migration Patterns Of 11 Antioxidants From Degradable Polylactic Acid Straws, Luo Minting, Wu Zhihang, Hu Tengxin, Xu Qingpeng

Food and Machinery

[Objective] To establish a method for authenticity identification of camellia seed oil. [Methods] The fatty acid composition of camellia seed oil and six common vegetable oils are determined by gas chromatography. Principal component analysis (PCA) is employed to explore the distribution patterns of fatty acids among the vegetable oils and to screen for potentially adulterated oils. Partial least squares discriminant analysis (PLS-DA) is applied to establish a model between fatty acid profiles and actual adulterated oils. Finally, multiple stepwise linear regression analysis is used to establish quantitative analysis models for adulteration in camellia seed oil. [Results] Among the six common …

Determination Of Five Fluorescent Whitening Agents In Prepared Dishes By Gel Permeation Chromatography-High Performance Liquid Chromatography, Li Bingbing, Wang Chunlei, Huang Zhenzhen, Dai Li, Wang Kun

Food and Machinery

[Objective] To establish a method for the determination of five fluorescent whitening agents (FWAs) in prepared dishes using gel permeation chromatography combined with high-performance liquid chromatography (GPC-HPLC). [Methods] The prepared dish samples were homogenized, ultrasonically extracted with acetonitrile, purified and reconstituted by GPC, then filtered before being analyzed by HPLC. The separation was performed on a Waters Symnetry Shield PR-C18 column (4.6 mm ×250 mm, 5.0 µm) using acetonitrile and water as the mobile phase with gradient elution. [Results] The five FWAs in the prepared dishes showed good linearity within the range of 2~500 µg/L, with correlation coefficients greater than …

Non-Destructive Detection Of Pork Loin Freshness Based On The Yolo V 11 Model, Deng Zhonghui, Xie Wei

Food and Machinery

[Objective] To develop an intelligent method for non-destructive recognition of pork loin freshness based on computer vision technology, which is to be achieved by establishing a classification model for accurate, fast, and non-destructive detection of pork freshness in the cold chain industry. [Methods] A classification detection model is proposed based on the YOLO v11 architecture. First, total volatile basic nitrogen (TVB-N) content and pH values are measured before the meat samples are divided into three categories: "fresh", "less fresh", and "spoiled". A dataset of 4 680 high-resolution images is constructed (generated from 1 247 original images enhanced through operations such …

Effect Of Lotus Leaf Powder Addition On Whole Wheat Dough Characteristics And Whole Wheat Bread Quality, Chen Rui, Gong Lingxiao

Food and Machinery

[Objective] To determine the optimum addition of lotus leaf powder in whole wheat flour, thus improving the nutritional value of whole wheat bread while maintaining high quality. [Methods] Rapid viscosity analyzer, rheometer, and texture analyzer are employed to explore the pasting properties of the composite flour, the rheological characteristics of the dough, and the texture properties of the bread. Additionally, the effect of lotus leaf powder on the quality of whole wheat bread is evaluated using a colorimeter, sensory evaluation, and in vitro digestion evaluation. [Results] The analysis reveals that as the lotus leaf powder addition is increased, a significant …

Effects Of Various Extraction Methods On The Performance Of Corn Fiber Gum-Pea Protein Composite Gels, Wu Chenglong, Jiang Zhili, Huang Lilin, Tang Leiming, Sun Xiaohong

Food and Machinery

[Objective] This study aims to investigate the effects of three extraction methods - alkaline extraction, enzymatic extraction, and acid extraction - on the properties of corn fiber gum (CFG)-pea protein composite gels. [Methods] CFG was extracted from corn bran using the three methods. The gel strength, water-holding capacity, rheological properties, and microstructure of the CFG-pea protein composite gels are evaluated. [Results] The CFG extracted by enzymatic extraction increases the gel strength of pea protein gels from (27.20±2.45) g to (65.80±2.67) g, and the water-holding capacity from (40.60±3.40)% to (75.80±5.10)%. Additionally, it forms a more compact and ordered network structure with …

Research Advances In Rice Bran Oil Bioactive Components And Antioxidant Technologies, Tu Yue, Guo Xiongchang, Zhang Wenhan, Cai Lichuang, Liu Jianping

Food and Machinery

Rice bran oil is a unique natural edible oil with healthy benefits and a reasonable fatty acid composition. Containing natural antioxidant components including phytosterols, oryzanol, tocotrienols, and squalene, rice bran oil is characterized by high nutritional value, diverse physiological functions, and excellent frying performance, thus demonstrating broad application potential. This study reviews the research advances in the bioactive components, physiological functions, factors influencing oxidative stability, and antioxidant technologies of rice bran oil, while providing prospects for future development.

Research Progress On Effect Of Salt On Pickling Vegetable Quality, Qi Kai, Li Ziyan, Cheng Wenbo, Fan Mingcong, Zuo Daoxi, Li Yan, Wang Li, Zhang Wenbin

Food and Machinery

Vegetable pickling is a time-honored food preservation and processing method, in which salt (primarily sodium chloride) plays an irreplaceable and core role. This paper systematically reviews the multidimensional effects of curing salt on vegetable quality. It comprehensively covers preservation mechanisms, textural transformations, the formation and conversion of flavor compounds, color stability, and nutritional value changes. Specifically, this article analyzes the decisive role of salt concentration gradients in final product characteristics. Furthermore, it examines the challenges posed by the low salinity, including microbial safety risks, textural deterioration, and flavor imbalances, while proposing corresponding strategies.

Research Progress In The Extraction And Purification Processes And Biological Activities Of Flavonoids From Cuscutae Semen, Li Renxin, Xiong Ying, Song Hui

Food and Machinery

Cuscutae Semen is a Chinese herbal medicine and a raw material for health foods in China, with flavonoids being a group of its main functional components. This article provides a systematic review of the extraction and purification techniques - reflux, ultrasonic, Soxhlet, microwave-assisted, flash, enzymatic hydrolysis, and supercritical CO2 extraction methods - of flavonoids from Cuscutae Semen. Furthermore, it elaborates on the biological activities such as reproductive protection, anti-tumor effect, anti-oxidative stress effect, liver and kidney protection, and immune regulation of flavonoids from Cuscutae Semen, as well as their related mechanisms. Moreover, this paper makes an outlook on the development …

Research Progress On Plant-Derived Metal Ion Chelating Peptides, Pei Hanzhe, Zhao Hongfei, Zhou Jiahua, Zhang Bolin, Chen Yonghao

Food and Machinery

Mineral element deficiency can cause many health problems. Traditional metal element supplements have many shortcomings. Food-derived metal element supplements have gradually become a research hotspot. Plant-derived polypeptides have significant advantages in terms of cost and raw material sources. Chelating peptides formed by chelating metal ions with plant-derived polypeptides have the physiological activity of peptides and the functional characteristics of metal elements, which makes them relatively ideal nutritional supplements, showing broad development prospects. This paper collects relevant research reports at home and abroad, and sorts out the preparation process, chelation mechanism and characteristics, and biological activity of plant-derived metal ion chelating …

Innovative Application Of Intangible Cultural Heritage Narrative Design In Agricultural Food Packaging, Chen Yanan, Chen Bingyan

Food and Machinery

cultural distinctiveness in current agricultural food packaging, this study puts forward an innovative approach to promote the upgrade of agricultural food packaging from functional consumption to cultural consumption through intangible cultural heritage (ICH) narrative design. In terms of methodology, the value of ICH narrative in food packaging is first analyzed, followed by the proposal of a design pathway encompassing three aspects: ICH content selection and story construction, narrative graphic and layout design, and material and process selection, with interactive experience being strengthened by integrating technologies such as the Internet of Things (IoT) and augmented reality (AR). In terms of application …

Enhancing Rapid Reef Resilience Through Transplantation Of Thermally Tolerant Corals In OʻAhu, HawaiʻI, Allison M. Klein

Electronic Theses and Dissertations

Coral reefs are increasingly threatened by marine heatwaves, disease outbreaks, and other anthropogenic stressors, leading to widespread declines in coral cover worldwide. As a result, efforts are increasingly focused on identifying thermally tolerant corals for reef restoration. However, it remains unclear whether thermal tolerance is maintained following transplantation and whether thermally tolerant corals perform differently in novel environments. This dissertation examined thermal tolerance, survivorship, symbiont communities, and growth in two dominant Hawaiian reef-building corals, Montipora capitata and Porites compressa, using a reciprocal transplant experiment between Kāneʻohe Bay and Ulupaʻu, Oʻahu. Initial heat-stress assays showed high thermal tolerance in both …

Biomechanical And Talar Cartilage Responses To Running In Individuals With Chronic Ankle Instability, Minsub Oh

Theses and Dissertations

Context: Chronic ankle instability (CAI) has shown altered movement patterns and joint loading, which increase the risk of posttraumatic ankle osteoarthritis. Abnormal vertical ground reaction force (vGRF) during running may contribute to talar cartilage degeneration; however, the acute biomechanical and cartilage responses to running in individuals with CAI remain unclear. Purpose: The purpose of this study was to compare running biomechanics and changes in talar cartilage thickness and T2 relaxation times before and after running between individuals with CAI and healthy controls. Methods: Twenty individuals with CAI and 20 healthy controls completed running biomechanics and magnetic resonance imaging (MRI) sessions. …

Irrigation Water Conservation And Accelerated Composting: An Exploration Of Environmentally Conscious Practices For Ornamental Horticulture, William James Carson Dunford

LSU Master's Theses

Best management practices for ornamental horticulture include proper management of irrigation water resources and substrate components. The first study investigated the effects of wind speed, wind direction, water pressure, and spray stake height on irrigation efficiency in nursery containers. Results indicated that lower spray stake height positions and lower wind speeds significantly improved the irrigation water volume captured within nursery containers by as much as 18% to 39%. Orienting spray stakes against prevailing winds further enhanced water retention, particularly at 0 and 1 inch heights. In contrast, higher stake elevations led to increased irrigation water loss by as much as …

Plant Diversity Modifies Multi-Trophic Interactions In Croplands, Grasslands And Forests, Nian Feng Wan, Muyao Li, Siyuan Shen, Jun Xiang Zhou, Yu Tong Zhu, Ben A. Woodcock, Yue Qing Hu, Shinichi Nakagawa, David Tilman, Andy Hector, Michel Loreau, Peter B. Reich, Matteo Dainese, Forest Isbell, Bernhard Schmid, Kris A.G. Wyckhuys, Geoff M. Gurr, Linsheng Wan, Nico Eisenhauer, Yann Hautier, Anu Eskelinen, Richard D. Bardgett, Paul Kardol, Lauchlan H. Fraser, Qi Su, Shiyun Qiu, Yavanna Aartsma, Ramon Albajes, Matthias Albrecht

All Peer-Reviewed Publications

Multitrophic interactions can strongly influence the structure and functioning of ecosystems, but how plant diversity influences the direction and predictability of multitrophic interactions across agricultural and natural ecosystems remains unclear. Using 149 field studies across five continents, we found that, on average, increasing plant diversity tended to exert differential top-down and bottom-up effects in croplands versus grasslands and forests. Organic and nonorganic croplands exhibited 846 and 148% higher invertebrate natural enemy-to-herbivore abundance ratios under increased plant diversity, consistent with top-down control patterns where predator gains cause herbivore declines, enhancing crop outcomes. In grasslands and forests, increasing plant diversity was associated …

Optimization Of Gemifloxacin Immediate-Release Tablets Using Quality By Design, Jong Woo Kim, Hyun Bon Kang, Yeun Hee Kim, Kang Min Kim

Journal of Food and Drug Analysis

The objective of this study is to determine the design space (DS) for a dissolution-enhanced gemifloxacin immediate-release (IR) tablet incorporating hydroxypropyl-β-cyclodextrin (HPCD) for improved solubility, utilizing a quality by design (QbD) approach. Based on the preliminary studies, hardness, friability, disintegration time, assay, content uniformity, and dissolution rate were identified as the critical quality attributes (CQAs) for optimization. Through the initial risk assessment, crospovidone, main compression force, and the amount of granulating fluid were identified as factors influencing the CQAs, and a 2³ + 3 full factorial design of experiments (DoE) was conducted for optimization. According to the analysis of variance …

Pharmacological Potential Of Pomelo (Citrus Maxima) Peel: A Bibliometric Review Of Global Research Trends And Future Directions, Indah Zunita Sari, Alma Nur Azizah, Fitriana Nur Hanifah, David Fernando, Agung Endro Nugroho, Abdul Rohman

Journal of Food and Drug Analysis

Pomelo (Citrus maxima) is a widely cultivated tropical fruit with global production reaching approximately 8.55 million tons in 2023. This large-scale production generates a substantial amount of peel waste despite the fact that pomelo peel contains secondary metabolites with promising pharmacological potential. This study aimed to evaluate global research trends related to the pharmacological potential of pomelo peel using a bibliometric and review-based approach, as well as to identify future research directions. Data were collected from the Scopus database and filtered for English-language scientific articles, reviews, and conference proceedings. The analysis was performed using the Biblioshiny and VOSviewer …

Plant By-Products As A Valuble Source Of Natural Antiglycemic Compounds: Economic Aspects And Alternative Raw Material Sources, Tatjana Đorđević, Jelena Vujetić, Isidora Dickov, Diandra Pintać Šarac

Journal of Food and Drug Analysis

Plant by-products, often considered as waste in various agricultural and food processing industries, have emerged as a valuable source of natural antidiabetic or antihyperglycemic compounds. These compounds, derived from plant materials that would otherwise be discarded, have the potential to mitigate the global burden of diabetes and offer sustainable solutions for glycemic control. Prominent groups of natural antidiabetic compounds found in plant by-products are polyphenols, as well as bioactive proteins and peptides. In addition, agro-industrial residues such as wheat bran, oat husks, and fruit peels are excellent sources of carbohydrates that can be incorporated into functional foods to help manage …

Emerging Antifungal Strategies Against Candida Albicans Using Metal–Organic Frameworks And Novel Therapeutics: Opportunities And Challenges, Amanpreet Singh, Sandeep Kumar, Sehrabpreet Singh, Jeewanjot Singh

Journal of Food and Drug Analysis

Candida albicans is an opportunistic fungal pathogen responsible for infections ranging from superficial mucosal disease to life-threatening systemic candidiasis, particularly in immunocompromised hosts. Its pathogenicity is enhanced by morphological plasticity, biofilm formation, and immune evasion, contributing to antifungal resistance. Limitations of existing antifungals have driven interest in emerging therapeutics. This review highlights strategies including metal–organic frameworks (MOFs), host-directed therapies (HDTs), CRISPR-Cas9 gene editing, and phytochemicals. MOFs offer high drug-loading capacity, controlled release, and enhanced penetration into biofilms, showing promise against drug-resistant strains. Selected phytochemicals demonstrate inhibitory effects on virulence traits such as hyphal formation and enzyme secretion. CRISPR-Cas9 …

An Optical Fiber Caffeine Sensor Using A Molecularly Imprinted Polymethacrylic Acid Membrane Coated On Optical Fiber Surface As A Transducer, Josiah W. Miller, Jason D. Mccaslin, Shiquan Tao

Journal of Food and Drug Analysis

Caffeine is a neurotransmitter, important to human health. This compound exists in many food products, has been added to soft drinks, pharmaceutical drugs. For many applications, a field deployable technique for quick detecting caffeine is attractive. In this paper, we report a selective optical fiber caffeine sensor using a molecularly imprinted polymer (MIP) membrane as a transducer. The membrane extracts/concentrate caffeine from sample solutions significantly improved sensor’s sensitivity with a 95 times sensitivity improvement. The sensor achieved a detection limit of 0.20 µg/mL. This sensor was used to directly analyze caffeine in soft drink products without interference from sample matrix.

Corrigendum To " Urban Transportation Strategy As Urban Sustainable Indicator Frameworks Study Of Masdar City, Abu Dhabi", Rania Badawy Shokry

Mansoura Engineering Journal

No abstract is required for this article.

Electroceuticals In Neuromodulation: Mechanisms, Clinical Applications, And Future Directions, Albashir Tahir, Nura Bello

AUIQ Complementary Biological System

Electroceuticals have emerged as an innovative therapeutic approach that employs targeted electrical stimulation to modulate neural circuits and restore physiological balance. Unlike conventional pharmacological treatments, which act through systemic biochemical pathways, electroceuticals enable precise spatial and temporal control of neural activity. The rapid proliferation of electroceutical devices across clinical and investigational settings, combined with accelerating advances in artificial intelligence, closed-loop engineering, and bioelectronic interface design, has generated an urgent need for rigorous, critically appraised synthesis to guide evidence-based clinical adoption and inform future research priorities. This review addresses the rapidly expanding field of bioelectronic medicine by providing a critical synthesis …

Systemic Infection Of Beauveria Bassiana In A Stranded Endangered Green Turtle Chelonia Mydas: A Case Report, Ming-An Tsai, I-Chun Chen, Tsung-Hsien Li

The Thai Journal of Veterinary Medicine

The green turtle (Chelonia mydas) is the most frequently encountered marine turtle species in Taiwan. This study describes the postmortem findings of a juvenile green turtle with injuries to the right foreflipper that was rescued off the Taiwan coast and died after 3 months in rehabilitation. Necropsy revealed large, yellow, multifocal caseous lesions involving the right foreflipper, spleen, liver, kidneys, and lungs. Fungal infection of Beauveria bassiana was confirmed by culture, morphological and molecular characterization, phylogenetic analysis, and histopathological examination. This report provides evidence that B. bassiana is associated with a systemic fungal infection which can be fatal …

Selenoprotein P And Selenium As Potential Diagnostic Biomarkers For Breast Cancer Patients, Farah A. Rashid, Rawaa A. Ali, Hanaa A. Ali

Karbala International Journal of Modern Science

Breast cancer incidence increases in last decade. Oxidative stress increases during cancer development causes consuming of antioxidants. Selenium and its transporting protein, selenoprotein P have an important role in redox homeostasis. This study aims to investigate whether or not selenium and selenoprotein P could be diagnostic biomarkers for breast cancer patients. This study was included 30 individuals in control group who is age- and BMI-matched with 60 breast cancer patients. Selenium was measured by colorimetric assay kit and selenoprotein P was determined by Sandwich ELISA kits. The results indicate significant decrease (p < 0.0001) of serum selenium and selenoprotein P in breast cancer patients compared to control. A positive significant correlation (r = 0.6214, p < 0.0001) was found between selenium and selenoprotein P in breast cancer patients. Selenium has an excellent diagnostic accuracy with area under curve 0.9831, sensitivity 95% and specificity 100% at cut-off less than 3.54 mmol/L. Furthermore, selenoprotein P has also an excellent area under curve 0.9958 with sensitivity 96.77% and specificity 100% at cut-off less than 4.15 ng/ml. These exploratory findings appear that selenium and selenoprotein P could be potential diagnostic biomarkers for breast cancer patients. Further investigations in large-multicenter populations are needed to confirm results.

Flexible Concurrent Training In Remote Settings, Christopher Bonilla

Doctoral Dissertations and Projects

The purpose of this study was to compare the effects of a trainee-driven flexible concurrent training program to a traditional fixed concurrent training program over 16 weeks of remote training in recreationally trained individuals. Participants were randomized into a flexible group that selected workouts based on daily perceived readiness or a control group that followed a fixed training schedule. The primary outcome was a total fitness score derived from a three-repetition maximum deadlift, a two-minute push-up test, and a two-mile run, assessed every four weeks. A secondary analysis used multilevel modeling to examine whether self-reported sleep duration predicted perceived readiness …

The Effects Of Anti-Gdnf On The Sensory And Autonomic Nervous System In The Heart, Tryphosa Konadu

Honors Theses

Heart disease is the number one leading cause of death; about 1 in every five deaths is due to heart disease. “Heart disease” is a catch-all phrase that encompasses a variety of conditions that affect the heart’s structure and function. Many of these symptoms can be attributed to dysfunction within the parasympathetic, sympathetic, and sensory nervous systems. Neurotrophic factors like GDNF (Glial cell line-derived neurotrophic factor), BDNF (Brain-derived neurotrophic factor), and NGF (Nerve growth factor) promotes neuronal survival, and regeneration of the nerve fibers of the heart, especially sensory and autonomic nerve fibers. Studies have suggested that increased expression of …

Lameness In Dairy Cattle: A Practical Overview, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet provides an overview of lameness in dairy cattle, including what it is, why it matters, and how to recognize and manage it. Information is geared toward producers, consultants, and educators.

Development Of Novel Plasminogen Activators From Fragments Of Streptokinase Enzyme, Vijay G Mr

Theses and Dissertations

Thrombotic disorders such as myocardial infarction, ischemic stroke, pulmonary embolism and deep vein thrombosis remain major causes of mortality and disability worldwide, creating a continuing need for effective, safe and affordable thrombolytic therapy. Streptokinase is one of the most widely used plasminogen activators because of its activity, low cost and broad availability. However, its wider clinical utility is limited by high immunogenicity, allergic reactions, neutralizing antibody formation and poor suitability for repeat administration. This thesis aimed to develop novel streptokinase-derived plasminogen activators by identifying smaller functional fragments that retain thrombolytic activity while potentially reducing immunogenicity.

A literature-guided fragment-based strategy was …

Progress And Prospective Of New Pollutants Study, Ruan Ting, Chunyang Liao, Yawei Wang, Maoyong Song, Yaqi Cai, Guibin Jiang

Bulletin of Chinese Academy of Sciences (Chinese Version)

The governance of new pollutants has been becoming a critical strategic demand for the high-quality development of China. The policy will be further advanced during the coming National 15th Five-Year Plan period. Nevertheless, significant challenges remain in aspects such as list of controlled chemicals, standard development, measurement methods, alternative approaches, and remediation techniques. Existing experience in addressing traditional pollutants remains insufficient to adequately address the concealed and complex ecological and/or health risks associated with new pollutants. Thus, a strengthening of basic research is the optimal approach to tackling these challenges and avoiding detours. To better identify the critical scientific challenges …