Digital Commons Network^™

Mutational Analysis Of Domain Functions In The Yeast Decapping Enzyme Dcp2, Jellisa Ewan, Ambro Van Hoof

Dissertations and Theses (Open Access)

In yeast, the major mRNA decay pathway involves deadenylation of the 3’ poly-A tail, followed by decapping of the 5’ m7Gppp cap and subsequent 5’ to 3’ degradation or 3’ to 5’ degradation. This is carried out by Pan2/Pan3 and Ccr4/Not deadenylases, the decapping enzyme Dcp2, and Xrn1 and  RNA exosome exoribonucleases, respectively. The eukaryotic mRNA decapping enzyme complex, Dcp1/Dcp2, is essential in yeast, and deletion of either gene is lethal. However, the deadenylases that act upstream and the 5’ exoribonuclease that act downstream of the decapping step are not, even though they are all highly conserved with no known …

Evaluating The Relationship Between Naltrexone Systemic Exposure And Acute Brain Response Using A Neuroimaging-Based Pharmacodynamic Biomarker, Trisha Rastogi, Stephani Stancil, Susan M. Abdel-Rahman, Michael Bartkoski, James Bartolotti, Mariah E. Brewe, William Brooks, Morgan Brucks, Anna Burns, Ann M. Davis, Laura Martin, Jeffrey R. Strawn, John Tumberger, Michaela Voss, Hung-Wen Yeh

Posters

No abstract provided.

Shared And Distinct Alterations In Brain Structure Of Youth With Internalizing Or Externalizing Disorders: Findings From The Enigma Antisocial Behavior, Adhd, Major Depressive Disorder, And Anxiety Working Groups, Sophie Townend, Marlene Staginnus, Yidian Gao, Nina Alexander, Volker Arolt, Tobias Banaschewski, Katja Beesdo-Baum, Mark A. Bellgrove, Vivek Benegal, Anderson M. Winkler

Human Genetics Publications

Background: Externalizing and internalizing disorders are common in youth but are often studied separately, preventing researchers from identifying shared (i.e., transdiagnostic) alterations in brain structure. Using data from the ENIGMA (Enhancing NeuroImaging Genetics through Meta-Analysis) Consortium, we conducted a mega-analysis to identify shared and distinct cortical and subcortical brain alterations across internalizing (anxiety disorders and depression) and externalizing (attention-deficit/hyperactivity disorder [ADHD] and conduct disorder [CD]) disorders in youth.

Methods: 3D T1-weighted magnetic resonance imaging data from youths (age range 4-21 years) with anxiety disorders (n = 1044), depression (n = 504), ADHD (n = 1317), and CD (n = 1172) …

Visual Cues Attract Adult Tunga Penetrans (Siphonaptera: Tungidae), Ayako Hyuga, Paul Ouma, Lynne Elson, Kazuchiyo Miyamichi, Satoshi Kaneko, Kensuke Goto, Tullu Bukhari, Jürgen Krücken, Ulrike Fillinger

All Peer-Reviewed Publications

Tunga penetrans (Linnaeus, 1758) (Siphonaptera: Tungidae) is an ectoparasitic flea whose adult females cause tungiasis by embedding into the host’s skin. Environmental, social and financial challenges in tungiasis endemic areas have left this disease neglected and lacking locally available control measures. One possible approach for controlling T. penetrans targets its off-host stages; however, surveillance tools for adult fleas are still lacking. Moreover, host-seeking cues and behaviour of adult T. penetrans remain largely unknown. In this study, we show that visual cues are attractive to adult T. penetrans. Newly emerged T.penetrans responded to light stimuli across a broad spectrum, ranging from …

Comparative Assessment Of Developmental Fidelity In Hpsc-Derived Neural Crest Cells, Michael H. Zepeda

Electronic Theses, Projects, and Dissertations

Neural crest cells (NCCs) are a transient, multipotent cell population that contributes to craniofacial structures, peripheral neurons, melanocytes, and smooth muscle. Defects in NCC development underlie a broad range of congenital disorders known as neurocristopathies. Human pluripotent stem cell (hPSC)-derived NCCs provide an evaluable model for studying early human development; however, existing differentiation protocols produce variable outcomes, and no standardized method consistently generates high-fidelity NCC populations. To address this challenge, we compared five published hPSC-to-NCC differentiation protocols using the H9 human embryonic stem cell line. We hypothesized that specific protocols more faithfully recapitulate neural crest development through coordinated regulation of …

Testing P300-Based Biometric Authentication Paradigms, Jordan Razzak

Electronic Theses and Dissertations

Bench research suggests that P300 amplitude is greater in response to self-relevant stimuli compared to familiar stimuli. This effect could enable a novel method of biometric authentication based on the self-relevant P300. This dissertation aims to: (1) determine the optimal presentation paradigm for leveraging self-relevance in P300-based biometric authentication, and (2) investigate P300 responses from self-relevant stimuli versus stimuli learned over the course of a week.

Two presentation paradigms were compared – rapid serial visual presentation (RSVP) and matrix presentation (MP). Participant-specific RSVP and MP classifiers were trained by applying stepwise linear discriminant analysis to data collected during presentations of …

Secondary Biology Teachers’ Understandings Of Growth Mindset, Kathryn Chapman

Dissertations

This case study investigates how high school biology teachers understand and apply growth mindset principles in their daily instruction. Using surveys, semi‑structured interviews, and focus groups, the study examines how biology teachers interpret mindset, how they attempt to enact it, and how curriculum demands shape their efforts. Findings show that teachers’ beliefs about intelligence strongly influence their feedback, expectations, and instructional decisions. Teachers described biology as a subject in which struggle is highly visible, emotionally charged, and often tied to students’ prior knowledge, making mindset work both necessary and challenging. Results also indicate that students’ mindsets are shaped by broader …

Melatonin-Functionalized Silver Nanoparticles Enhance Salinity Tolerance In Brassica Juncea By Modulating Antioxidant Defence And Growth Physiology, Mohini Yadav, Manar Fawzi Bani Mfarrej, Vikash Kumar, Ankita Singh, Faheem Ahmad, Saurabh C. Saxena

All Works

Soil salinity is a major abiotic stress that severely limits plant growth and productivity by inducing osmotic stress, oxidative damage, and ionic imbalances. The present study evaluated the potential of melatonin-mediated silver nanoparticles (Mel AgNPs) to enhance salinity stress tolerance in Brassica juncea. Pot experiments were conducted using 21-day-old plants exposed to salinity stress and treated with Mel AgNPs at different concentrations (50 and 100 mg L⁻¹) through both root application and foliar spray. Morphological, physiological, and biochemical responses were assessed 48 h after nanoparticle exposure. Salinity stress markedly increased lipid peroxidation and hydrogen peroxide accumulation while suppressing antioxidant defence. …

Temporal Dynamics Of Sensorimotor Integration For Auditory And Proprioceptive Target Information During Movement Preparation And Execution, Ivan Camponogara

All Works

Actions toward non-visual targets exhibit modality-specific patterns of endpoint variability, with movements toward auditory targets being more variable than those toward proprioceptive or multisensory targets. However, whether such modality-specific effects emerge already during movement preparation and how they evolve across movement execution remains unclear. The present study addressed this question by asking participants to perform reaching movements toward auditory, proprioceptive, or audio-proprioceptive targets while electromyography and movement kinematics were recorded. Movement preparation and initiation was assessed by analyzing two temporal components of reaction time, premotor and motor, together with movement kinematics at peak acceleration. Movement execution was investigated by analyzing …

Advancing Management Strategies For Two Promising Mason Bee Species (Osmia Ribifloris And Osmia Bruneri) For Berry Pollination, Miranda L. Jones

All Graduate Theses and Dissertations, Fall 2023 to Present

Bee management can broadly refer to human manipulation of one or multiple parts of a bee’s lifecycle. Managing honey bees, commonly referred to as beekeeping, is responsible for providing most pollination for global food crops. However, using more than one bee species for crop pollination can have many benefits. Benefits include better crop pollination and insurance in case one bee species underperforms or its population collapses. Some bees within the genus Osmia are already widely managed for crop pollination because their developmental lifecycle allows bee managers to readily deploy pollinating bees in sync with varying crop blooms, and for certain …

Nh Dfl - 25 Years Of Hwa - Peterborough_2026_Aug_11, Ryan Crandall

UNH Cooperative Extension

No abstract provided.

Harnessing Kenyan Aquaculture Through Social Media: Impacts, Challenges And Strategic Directions, Domitila Kyule, Jonathan Munguti, Mavindu Muthoka, Esther Magondu, Menaga Meenakshisundaram, Chrysantus M. Tanga, Kevin Obiero, Cecilia Githukia

All Peer-Reviewed Publications

Aquaculture is increasingly important for food, nutrition and livelihoods in Kenya, but the sector still faces persistent constraints in extension delivery, farmer learning, input access, market coordination and information reliability. This review examines how social media and related digital communication tools are shaping aquaculture development in Kenya, with attention to impacts, challenges, governance needs and strategic directions. A systematic literature review of studies and institutional sources published between 2015 and 2025 was conducted. The search identified 388 records, of which 45 sources were included in the final synthesis after screening, eligibility assessment and quality appraisal. Because the evidence was heterogeneous, …

Seizure Susceptibility And Morphological Analysis Of A Missense Mutation In Hcfc1a Protein, Jose Manuel Fabela

Open Access Theses & Dissertations

Missense mutations in the HCFC1 gene cause methymalonic acidemia with homocystinuria cblX type (MIM#309541). cblX is an X-linked disorder that is associated with multiple phenotypes such as metabolic defects, failure to thrive, intractable epilepsy, facial dysmorphia, movement disorders, and intellectual disability. The mechanisms underlying these phenotypes are not completely understood. Here we used a zebrafish germline mutant allele (hcfc1aco64/co64) to study seizure susceptibility and adult brain structural malformations. Previous studies have established neural progenitor cell defects and hypoactive mechanistic target of rapamycin (mTOR) in this allele. Given the known role of mTOR in seizure phenotypes, we hypothesized that missense mutation …

Expression Levels Of Heat Shock Protein 72 And Their Correlation With Biomarkers Of Renal Function Among Hispanic Construction Workers Exposed To Ambient Heat In El Paso, Texas, Maria Fuentes

Open Access Theses & Dissertations

Heat shock proteins (HSP) are intracellular molecular proteins that are upregulated in response to cellular stress, including heat exposure. Through their protective functions, HSP help maintain protein structure, prevent protein denaturation, and preserve cellular homeostasis during states of physiological stress. Because of their cytoprotective properties, HSP have emerged as potential biomarkers for detecting early signs of tissue injury and predicting clinical outcomes associated with heat related illnesses (HRI), including acute kidney injuries (AKI). AKI is a leading cause of hospitalizations due to heat stress exposure and heat stroke. In the absence of early detection and effective therapeutic interventions, AKI may …

Parametric Assessment Of Nicotine Vapor Self-Administration In Rats, Tiffany Alexandra Gonzalez-Gutierrez

Open Access Theses & Dissertations

The increasing prevalence of electronic cigarette (e-cigarette) use among adolescents highlights the need to identify factors that promote nicotine vapor use in populations most susceptible to its long-term consequences. This requires translationally relevant animal models of nicotine vapor self-administration (VSA). Accordingly, the goal of this dissertation was to evaluate 2 key factors hypothesized to influence nicotine VSA in rats, including age and e-liquid vehicle composition. Given recent mandates on studying sex as a biological variable, the present work included both female and male rats. Aim 1 compared VSA behavior during adolescence (adolescent-onset) or adulthood (adult-onset) in groups that received vapor …

Integrating Transcriptomics And Predictive Analytics For Multi-Cancer Prognostic Modeling Across Demographic Subgroups, Arnav Joshi

Open Access Theses & Dissertations

Cancer prognosis is shaped by complex molecular and clinical factors that challenge survival modeling using high-dimensional genomic data. Transcriptomic profiling provides a means to characterize molecular patterns associated with disease aggressiveness, yet the behavior of transcriptome-informed predictive models remains sensitive to endpoint choice, cohort structure, and demographic stratification. This dissertation presents a computational framework for transcriptomic analysis and survival modeling across multiple cancer types with explicit race-aware design. Using bulk RNA-sequencing data from The Cancer Genome Atlas, transcriptomic analyses were performed for breast invasive carcinoma (BRCA), lung adenocarcinoma (LUAD), prostate adenocarcinoma (PRAD), and liver hepatocellular carcinoma (LIHC). Differential expression and …

Optimizing Urban Forestry Through Green Infrastructure: Tree Health And Water Retention In Ciudad Juarez Rain Gardens, Tomas Rogelio Lizarraga Ruiz

Open Access Theses & Dissertations

Urban green spaces (UGS) in arid regions face significant challenges due to water scarcity and extreme thermal demand. This study evaluates the efficacy of Nature Based Solutions (NBS), rain gardens and median strips, in enhancing the physiological resilience of urban trees in Ciudad Juárez, Mexico. Using a comparative framework, the research monitored a native evergreen (Dermatophyllum secundiflorum) and an introduced deciduous species (Morus sp.) across six planting treatments. Statistical analysis reveals that green infrastructure (GI) acts as a critical physiological accelerant. Trees situated within rain gardens and medians significantly outperformed those in restricted pot and sidewalk settings, maintaining higher stomatal …

Tissue-Specific And Sex-Dependent Microbiome Structure In East African Cattle Ticks (Amblyomma Gemma And Rhipicephalus Pulchellus) And Their Bovine Hosts, Dennis Getange, Samson Mukaratirwa, Rua Khogali, Epaphrus Yuko, James Kabii, Joel L. Bargul, Jandouwe Villinger

All Peer-Reviewed Publications

Ticks and their vertebrate hosts share intimate ecological interactions that shape microbial community composition, with implications for tick fitness and pathogen transmission. However, the extent and specificity of tick–host microbial interactions remain poorly understood, particularly in East African tick species. Here, we characterised microbial communities across multiple tissue types from two economically important tick species (Amblyomma gemma and Rhipicephalus pulchellus) and their shared bovine host using 16S rRNA V1-V2 gene sequencing. We profiled haemolymph, midgut, salivary glands, and gonads from individual ticks (25 Am. gemma and 8 Rh. pulchellus), alongside 12 bovine blood samples. Microbiota were dominated by Proteobacteria, Firmicutes, …

Performance Of The Denitrification-Decomposition Model In Simulating Agronomic Properties And N2o Emissions Under Smallholder Climate-Smart Farming Systems, Esphorn Kibet, Collins M. Musafiri, Milka Kiboi, Onesmus K. Ng'etich, David K. Kosgei, Abdirahman Zeila, Franklin Mairura, Felix K. Ngetich

All Peer-Reviewed Publications

The DeNitrification-DeComposition (DNDC) model is a crucial tool for estimating soil greenhouse gas fluxes and understanding soil-plant interactions. This study evaluates the performance of the DNDC model in simulating soil temperature, moisture, crop yield, and nitrous oxide (N2O) fluxes across different land utilization types in Western Kenya. The land utilization types included: i) agroforestry M (agroforestry with Markhamia lutea, ii) sole sorghum, iii) agroforestry L (agroforestry with Leucaena leucocephala), iv) sole maize, and v) grazing Land, each replicated thrice. Fertilizer and manure were applied as part of the management practices, with manure applied at 2t ha⁻¹. Soil greenhouse …

The Influence Of Ecological Pest Management Practices On Pests And Natural Enemies Under A Cabbage–Garden Pea Rotational System In Central Kenya, Peter Nyakundi Mageto, Milka Kiboi, Monicah Mucheru-Muna, Felix Matheri

All Peer-Reviewed Publications

Pest pressure is a major constraint on crop production in Sub-Saharan Africa, where smallholder farmers rely heavily on synthetic pesticides with potential environmental and health risks. Ecological pest management practices (EPMs) have been proposed as sustainable alternatives, although field-based evidence is limited. This study evaluated pest and natural-enemy responses to EPMs in a cabbage–garden pea rotation system over six years (2020–2025) in Central Kenya. Four treatments were assessed: homemade botanical extracts (Botanicals), coriander intercropping (Intercrop), botanical extracts combined with coriander intercropping (Botanicals + Intercrop), and a control. In cabbage, ecological treatments often recorded lower aphid and diamondback moth abundance and …

An Analysis Of Mating Strategies In Rotifers Inhabiting Temporary Habitats, Ariel Bollinger

Open Access Theses & Dissertations

Reproduction is critical for organisms inhabiting ephemeral aquatic habitats where desiccation risk favors rapid and successful mating. Monogonont rotifers rely on sexual reproduction to produce diapausing eggs that withstand harsh conditions. I investigated how mating behaviors vary among populations of five rotifer species based on the hydroperiod of their habitat: Epiphanes brachionus and E. chihuahuaensis were sampled from habitats with short (SH) and long (LH) hydroperiods; Euchlanis dilatata was collected from a short (SH) and a permanent habitat; and Plationus patulus and Sinantherina socialis were collected from a long (LH) and a permanent (Perm) habitat. I hypothesized that SH populations …

Engagement Of Understudied Populations As Community Change: The South Texas Adrc Model, Gladys E. Maestre, Neela K. Patel, Rosa V. Pirela, Robin C. Hilsabeck, Kendra Stine, Neyra García, Roberto Reyes, Omar Oropeza, Cristian Maestre, Jesus D. Melgarejo, Sarah Williams-Blangero, Ney Alliey Rodriguez

School of Medicine Publications

Many populations experiencing the highest burdens of Alzheimer's disease and related dementias remain understudied, in part because traditional recruitment and retention models are insufficient to support sustained engagement. The Outreach, Recruitment, and Engagement Core (OREC) of the South Texas Alzheimer's Disease Research Center reconceptualized participant recruitment as a long-term system change in a Hispanic-majority, urban–rural region. OREC institutionalizes shared power through community advisory governance, aligns research protocols with local lived realities (through patient navigators, community health workers/promotores, and decentralized access points), and employs a data-driven feedback loop for continuous improvement. In 1 year, 46 outreach events touched 10,134 individuals (71% …

Irrigation With Sewage -An Effective Procedure For In­Creasing The Yield Performance Of Grassland As Well As For Protecting Inland Waters Against Pollution, V M. Novikov, N A. Kovaleva, V P. Sayapin, L F. Mikheeva, M I. Demina

IGC Proceedings (1977-2023)

The utilization of sewage for irrigation pur­poses is an effective procedure for increasing the yield performance of grassland as well as for protecting inland waters against pollution. Compared with irrigation only during the growing season, all-season use of sewage is more effective in increasing forage production and reducing both the quantity of sewage diverted into inland waters and the deficit of irrigation water. By way of science-based choice of the irri­gation regime, the perennial forage plant spe­cies, and their kind ofutilization, 8.0-10.0 tons and more of high-quality dry matter can be obtained per hectare, with 6,000-7,000 m3 of sewage being …

Effect Of Nitrogen Fertilization Of Pastures On The Cu, Fe And Zn Content In Fodder And Blood Of Dairy Cows, Kazimerz Rybak

IGC Proceedings (1977-2023)

Results of one of the grazing experiments carried out in the period 1971-1975 are dis­cussed. In the experiment two rates of N fer­tilization were applied: 60 and 120 kg per hectare under each regrowth, i.e. 360-420 and 720-840 kg N/ha per year, respectively. Rates of phosphorus and potassium fertili­zation remained unchanged: 120 kg P2O5 and 160 kg K2O per hectare. High N rates led to an increase of the Cu and Zn contents and an Fe content decrease in plants, and stimulated the Cu and Fe absorption but reduced the Zn absorption by high-yielding cows. A drop of the Cu …

Effect Of High Nitrate Fertilization On The Yield And Biological Value Of Grasses Tested By Common Vole, Slawomir Pronczuk, Janina Gnat, Tadeusz Szuperski

IGC Proceedings (1977-2023)

The effect of different fertilization doses ( 4 x 60, 4 x 120, and 1 x 480 kg N/ha) on the yield of grasses and their basic chemical composition as well as on the growth efficiency and health of animals fed those grasses was studied. Yield of grass during three following years was higher from the fields fertili_zed 4 x 120 and 1 x 480 kg N/ha, when compared to 4 x 60 kg N/ha. No differences were found be­tween 1 x 480 kg N/ha and 4 x 120 kg N/ha. Growth ·efficiency of the common voles which were fed …

Young-Cattle Rearing Along Industrial Lines And Pasturing In The Gdr, H Kleiber, F Morchen, F Lemme

IGC Proceedings (1977-2023)

Young-cattle rearing along ind us trial lines involves a general increase ofrearing standards. When pasturing large herds of young cattle it is difficult to achieve high live weight gain. Factory-like milk production requires not only optimal live weight development of the heifers but also an excellent state of health of the animals' legs. The latter is achieved more easily through pasturing than by way of indoor rear­ing of the youngstock. The intensification of young-cattle rearing includes the intensifica­tion of pasture rearing as well. Young-cattle rearing along industrial lines brings up new problems to be solved by technological re­search workers.

Factors Affecting Green Forage Intake By The Milking Goat, Lionel Huguet, Michel De Simiane, Claude Bernard Broqua

IGC Proceedings (1977-2023)

For the past fifteen years in France goat rearing has developed thanks to a favourable economic background. The specialization of farmers has given rise to new needs in breed­ing techniques and particularly in green forage feeding systems. From 1972 to 1975, trials were carried out to specify the utilization of green forage by milking goats in order to simplify and rationalize their diet. Three factors were studied to estimate their effect on forage intake: removal of hay from the ration, the quantities of green forage offered, and the quantities of concentrates distributed. When the green forage has a good feeding …

Re-Examining Endemic Smoky Dace As An Indicator Species In The Upper Little Tennessee River Index Of Biological Integrity, Tayton C. Alvis, William O. Mclarney, Radmila Petric, Jason P. Love

Southeastern Fishes Council Proceedings

Fish communities can be used to evaluate water quality and stream ecosystem health, helping resource managers understand the effects of anthropogenic disturbance. Fish species inform indexes of biological integrity through their ecological traits and tolerance to stressors (e.g. tolerant of disturbance, intolerant of disturbance), but these designations are difficult to determine without localized, species-specific information. The Upper Little Tennessee River watershed is subject to long-term aquatic biomonitoring, applying an index of biological integrity tailored to the region. In this index, the undescribed endemic Smoky Dace (Clinostomus sp.) is classified as intolerant based on a previous designation for the Rosyside Dace …

Phytochemical Analysis And Safety Assessment Of Watercress Nasturtium Officinale: Antioxidant Capacity, Heavy Metal Profiling, And Toxicity Using Brine Shrimp Model, Qurrota A’Yunin, Chamini Kasun Hemachandra, Muhaimin Rifa’I, Yoga Dwi Jatmiko, Maftuch Maftuch, Rizky Fadilla Agustin Rangkuti, Cucun Herlina, Zulfa Mahdiatur Rasyida, Moh Dliyauddin

Karbala International Journal of Modern Science

Medicinal plants have attracted growing attention as sources of natural bioactive compounds for sustainable aquaculture and health. This study evaluated the phytochemical composition, antioxidant activity, heavy metal profile, and acute toxicity of watercress (Nasturtium officinale W.T. Aiton) methanol extract using maceration, qualitative and quantitative (TPC, TFC, antioxidant DPPH) phytochemical assay, XRF, and BSLA. Phytochemical screening of plant extracts showed the existence of flavonoids, alkaloids, phenols, tannins, triterpenoids, and saponins, but not steroidal. The TPC and TFC were 20.6 mg GAE/g and 10.8 mg QE/g, respectively. The extract displayed significant antioxidant activity (IC₅₀ = 64.78 µg/mL in DPPH assay). …

Systematic Review Of The Institutionalization Of Service Learning In Higher Education, Wilmer Hernan Cruz Gutierrez

ASEAN Journal of Community Engagement

An increasing number of universities are integrating service-learning (SL) to fulfill their social mission. However, sustainable institutionalization of SL remains a persistent challenge. This study identifies and analyzes the factors, actors, and strategies that drive and inhibit SL institutionalization in higher education. Using the PRISMA 2020 guidelines, a systematic literature review analyzed 52 documents from seven academic databases (including WoS, Scopus, and ERIC). The findings were grouped into three sections: cultural (integration into institutional values/missions), process (articulation within teaching, research, and outreach), and structural dimensions (dedicated support entities). The results obtained reveal a reciprocal relationship where cultural alignment enables processual …