Digital Commons Network^™

Structural And Functional Studies Of The Sars-Cov-2 Niran Domain, Gabriel I. Small

Student Theses and Dissertations

The COVID-19 pandemic was a dramatically disruptive event, wreaking devastation across the globe and radically impacting nearly every facet of human life. For the first time, a significant amount of global money and attention was directed at coronavirus (CoV) research, propelling the field to the forefront of biomedical research. This allowed for the rapid development of a suite of vaccines and antivirals targeting SARS-CoV-2 that aided in ameliorating the effects of the pandemic. SARS-CoV-2 was the third CoV zoonosis of the 21st century and is now endemic. Global understanding of the threats posed by CoVs circulating among animals in the …

Dining In The Dark Atp Sensing In The Female Aedes Aegypti Mosquito, Katherine Adriana Rosas Villegas

Student Theses and Dissertations

Blood feeding is fundamental for female Aedes aegypti mosquitoes, which require this protein-rich meal for egg development. After being attracted to a human host by sensory cues such as body odor, body heat, and carbon dioxide exhaled in human breath, the mosquito lands and probes under the skin to feed on blood. Probing uses the stylet, a sharp appendage ensheathed in the mosquito labium that punctures the skin and is highly specialized for blood ingestion. Previous work from the lab with GCaMP calcium imaging of stylet neurons showed that approximately 50% of stylet neurons respond to blood, and that these …

The Complexities Of The Bilingual Advantage, Melisa Sierra

Behavioral Neuroscience Honors Papers

The bilingual advantage hypothesis, which proposes an enhancement of executive functions in bilinguals, remains heavily debated with inconsistent findings across studies. This study investigated working memory performance in bilingual and monolingual young adults using a modified n-back task with an interruption while recording EEG activity. Participants completed 1-back, 2-back, and 3-back conditions across the pre-interruption phase and a post-break 2-back condition. Behavioral measures (accuracy and response times) were analyzed alongside ERP, focusing on P300 and sustained activity components, were analyzed. Results showed a decrease in accuracy as task difficulty increased from 1-back to 3-back, indicating the expected effects of higher …

Building Public Health Data Dashboards: Tutorial Playbook, Elwin Wu, Raymond Balise, Benjamin Katz, Daniel R. Harris, Matthew Bullard, Naleef Fareed, Marc Larochelle, Jennifer Villani

Institute for Biomedical Informatics Faculty Publications

Public health data dashboards have substantial potential to improve transparency, understanding, and decision-making at multiple levels, from individuals to public health practitioners and policymakers. However, creating effective dashboards presents many challenges. In this case-based tutorial on public health dashboard development, we share lessons learned from our experience developing data dashboards for the HEALing Communities Study (HCS), a National Institutes of Health (NIH)–funded, community-engaged intervention to deploy evidence-based practices to reduce opioid overdose deaths in 67 communities across 4 states. We present key decision points dashboard teams must address, along with the major considerations and trade-offs that shaped our approach. First, …

Triangulating Primary Sources, Professional Judgement, And Llm-Generated Summaries: Educating Nurses In An Ai-First World, Sarah Oerther, Daniel B. Oerther

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

No abstract provided.

Rewilding The Coral Reefs, Magali Rodriguez, Luciana Alvillar

Posters - 2026

Coral reefs are among the most valuable ecosystems globally, contributing an estimated $10 trillion annually and supporting hundreds of millions of people through food, livelihoods, and coastal protection. (NOAA 2026) They also provide critical habitat for diverse marine species. However, reefs are rapidly declining due to rising ocean temperatures, acidification, pollution, invasive species, and physical disturbances. Globally, 30–50% of coral reefs have already been lost(NOAA 2026), and without intervention, many may face extinction by the end of the century. Rewilding and restoration efforts have become essential strategies to address this crisis. Initiatives such as NOAA’s Coral Reef Conservation Program highlight …

Impacts Of Native And Nonnative Plant Species On Avian Community Diversity, Matty John Mackay, Steven Van Dang, Brandon Lee Corn

SPARK Symposium Presentations

Avian communities play an essential role in maintaining a healthy ecosystem dynamic by regulating insect populations, assisting in plant pollination, and seed dispersal. These contribute to the overall health of natural ecosystems and mitigate ecological disruptions that would arise in their absence. However, the introduction of non-native invasive plant species can interrupt the balance avian communities bring to ecosystems by reducing availability of food sources, altering habitat structures, increasing competition among individuals, and disrupting ecological relationships through rapid invasion of ecological spaces. Point count observations of birds at Shelby Bottoms Park, Nashville, Tennessee in areas dominated by non-native privet and …

Leveraging Research-Based Knowledge Translation Via Rural Libraries To Improve Maize Postharvest Practices In Uganda, Jackline Estomihi Kiwelu, Patrick Ngulube

Libraries

Background: Rural community libraries are increasingly recognised for providing localised knowledge, yet their potential to support agricultural extension services and improve access to postharvest knowledge in Uganda remains underexplored.
Objectives: This study examined the postharvest information needs of smallholder maize farmers and explored how community libraries can be leveraged to translate and repackage research-based knowledge to meet these needs.
Method: A mixed-methods parallel design was employed. Data were collected from a survey of 312 maize farmers, personal interviews and two focus group discussions (FGDs) with 22 extension workers and four librarians. Desk research was conducted to analyse relevant documents. Quantitative …

Chronic Alcohol Consumption Enhances The Differentiation Capacity Of Hematopoietic Stem And Progenitor Cells Into Osteoclast Precursors, Hami Hemati, Madison B. Blanton, Jude Koura, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis. In a rhesus macaque model of voluntary ethanol consumption, CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response. In the present study, those studies were extended to investigate alterations in osteoclast development, which, in postnatal life, differentiate from HSPCs and play a critical role in maintaining bone homeostasis. Spectral flow cytometry revealed a skewing of HSPCs toward granulocyte-monocyte progenitors in the CAC group, consistent with an increased …

Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications To Promote Osteoclastogenesis, Hami Hemati, Brianna M. Doratt, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption increases the risk of osteoporosis and fracture by disrupting bone remodeling, in part by enhancing osteoclastogenesis. However, the cellular mechanisms underlying this process remain incompletely defined. We analyzed scRNA-seq data from osteoclasts differentiated in vitro from bone marrow mononuclear cells obtained from macaques following 12 months of chronic ethanol or isocaloric control solution consumption. Module scoring, trajectory inference with generalized additive modeling (tradeSeq), and CellChat-based analyses of intercellular communication were applied to uncover ethanol-induced changes in metabolic reprogramming, lineage progression, and signaling network dynamics. Module scoring indicated metabolic reprogramming toward oxidative phosphorylation, with reduced glycolytic, migratory, and …

Alcohol Consumption Exacerbates High-Fat Diet- Mediated Disruptions In Myelopoiesis And Osteoclastogenesis In Mouse Models Of Metabolic Dysfunction-Associated Liver Diseases, Hami Hemati, Madison B. Blanton, Lauren Rutt, Nicholas Keiran, Rebecca Geron, Florence Lima, Rebecca L. Mccullough, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Objective: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and Metabolic Dysfunction-Associated Alcohol-related Liver Disease (MetALD) exhibit systemic immune abnormalities. Given that such immune dysregulation is closely linked to the skeletal complications frequently observed in MASLD and MetALD, we sought to comprehensively characterize the bone marrow hematopoietic compartment and its link to osteoclastogenesis.

Methods: We utilized bone marrow from mouse models of MASLD (high-fat diet, HFD) and MetALD (high-fat diet plus ethanol, HFD+EtOH), followed by flow cytometric analysis to phenotype hematopoietic stem and progenitor cells (HSPCs), as well as in vitro and in vivo assays to evaluate osteoclastogenesis.

Results: We found that …

Fecal Microbiota Transplantation Modulates Gut Microbiota And Lipid Metabolism To Reduce Diarrhea In Low-Birth-Weight Neonatal Calves, Zhanhe Zhang, Duo You, Donglin Wu, Xintong Li, Yang Jia, David L. Harmon, Ming Xu

Animal and Food Sciences Faculty Publications

This study aimed to elucidate the mechanism by which colonic fecal microbiota transplantation (FMT) improves lipid metabolism in low-birth-weight neonatal calves via modulation of the gut microbial ecosystem. In a completely randomized design, seventy newborn Holstein calves (1 day old, 32.63 ± 3.13 kg BW) were assigned to two groups (35 calves/treatment; 30 females and 5 males per group). A healthy calf was used as a fecal donor. A 10% fecal bacterial suspension was prepared and administered colonically (50 mL, twice daily for 5 days). All calves were fed the same milk replacer and weaned using a step-down protocol at …

The Role Of Body Composition In Predicting Fitness Performance Of Law Enforcement Officers, Taylor J. Parmentier

Theses and Dissertations

Law enforcement officers (LEOs) perform physically demanding tasks such as foot pursuits, lifting, carrying, dragging, and physical restraint. Effectively performing these duties requires significant muscular strength, endurance, and cardiorespiratory fitness. Lower fitness levels pose risks for reduced health, performance, slower recovery, increased fatigue, and potentially affecting decision-making under strenuous conditions. The purpose of this study was to identify the strongest predictor of physical fitness performance among active-duty LEOs. Body composition analysis and a comprehensive fitness evaluation of twenty-nine male LEOs (age: 36.2 ± 9.3 years, body mass (BM): 95 ± 13.7 kg, height: 1.8 ± 0.1 m) was completed. The …

A Rare Case Of A Silent Pheochromocytoma Concomitant With An Incidentally Found Small Bowel Carcinoid Tumor, Michael Salzle, Anna Szafran-Swietlik, Tobias Carling

West Florida Division GME Research Day 2026

No abstract provided.

Bpa: Effects Of The "Plastic Era", Bianca G. Pietrangeli

Student Work

Infographic review of sources of BPA and its impacts on human health with a focus on osteoporosis.

Trustworthy Multimodal Ai For Medical Imaging: Enhancing Diagnosis, Reasoning, And Human-Agent Interaction In Extended Reality, Jai Prakash Veerla

Computer Science and Engineering Dissertations

The transition from traditional microscopy to digital pathology has digitized diagnostic data, yet clinical workflows remain constrained by two-dimensional screens and passive, opaque analysis tools that fail to capture the spatial complexity of biological systems. While Foundation Models now promise to reason across histology and genomics, a critical disconnect persists between the richness of this data and the limited cognitive bandwidth of clinicians, who currently lack the immersive interfaces and trustworthy agents necessary to utilize it effectively. This dissertation presents a unified framework for "Embodied Agentic AI," establishing a pipeline that augments physician capabilities through immersive visualization, robust security, and …

Toxic Torts Are In Your Blood: An Examination Of Pfas And Tort Liability, Aiden M. Holden

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Abstract

Per- and polyfluoroalkyl substances (PFAS), are a group of chemicals widely manufactured for industrial and commercial applications in the past decade due to their remarkable stability. Despite the “useful” nature of PFAS in various applications, they represent one of the most profound environmental and public health crises of the modern era. The chemical stability of PFAS has made them almost completely resistant to natural degradation, earning them the moniker “forever chemicals.” PFAS forever haunt our plant, contaminating the soil, water, and the blood of approximately 95% of the United States population. As the scale of corporate malfeasance by chemical …

Know Fear: The Culture Of Fear In Community Conversations, Penelope Meredyk, Lotus Porte-Moyel, Fiona Carson, Cosley Bruno, Barrett Clement, Sophia Sproull, Morgan Davis

Undergraduate Theses, Professional Papers, and Capstone Artifacts

“K(no)w Fear” is an award winning piece of verbatim theater based on word-for-word interviews and collected research that's goal was to cultivate a connective and thoughtful community open to exploring what shapes us all: fear. Our GLI Capstone team gathered the resources for this theater piece by conducting interviews with a mixed demographic of people we know as well as strangers on the street, asking questions like: “How do you feel about death?” and “If fear were a place for you, where would it be?” With consent, their answers were used to build an immersive, verbatim theatre piece where actors …

Corals, Creation, And Conservation: Evaluating Human-Marine Relations Through Coral Reef Biology And Artistic Practice, Emma Gray Garman

Scripps Senior Theses

This thesis investigates the relationship between humans and marine ecosystems through two complementary lenses: scientific research and artistic practice. The biology chapter proposes a five-year coral reef monitoring study across four Queensland, Australia islands — two heavily visited and inhabited (Fitzroy and Green Islands) and two minimally visited and uninhabited (High and Woody Islands) — to assess whether human activity correlates with bleaching severity over time. Grounded in preliminary survey data from Green Island in 2024, which revealed approximately 54% of coral bleached or dead, the study hypothesizes that reefs adjacent to inhabited islands will exhibit greater bleaching severity, consistent …

Transcriptional Signatures Of Rapid Protection From Sudan Virus Infection By A Single Dose Of A Vesicular Stomatitis Virus-Based Vaccine, Brianna M. Doratt, Sheridan B. Wagner, Delphine C. Malherbe, Gregory T. Smith, Andrea Marzi, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Sudan virus (SUDV) has caused multiple outbreaks of human disease with case fatality rates ranging from 41 to 100%. We have previously shown that a single vaccination with a recombinant vesicular stomatitis virus-based vaccine expressing the SUDV-Gulu glycoprotein (VSV-SUDV) prevented clinical and fatal disease from lethal SUDV challenge in cynomolgus macaques. With the high probability of future outbreaks, it is critical to determine the molecular mechanisms of VSV-SUDV-mediated protection and the ability to impart rapid protection against SUDV infection. In this study, RNA from whole blood samples obtained from nine cynomolgus macaques that were challenged with SUDV-Gulu post-vaccination with either …

Characterizing Immune Perturbations In Peripheral Blood Following The East Palestine, Ohio Train Derailment, Sheridan B. Wagner, Hami Hemati, Siva Gandhapudi, Timothy J. Hilbert, Candace J. Brancato, Erin N. Haynes, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Background: The East Palestine, Ohio, train derailment on February 3rd, 2023, resulted in the exposure of residents and the surrounding area to numerous hazardous chemicals, which included known acute irritants and human carcinogens. Despite evacuation and cleanup, both residents and responders reported high occurrences of symptoms associated with exposure in the months following, such as eye, skin, and respiratory irritation, headaches, and fatigue. The long-term health consequences for residents remain uncertain.

Objective: To assess the potential for immune perturbations resulting from the exposure, we performed a broad investigation of circulating immune cells and mediators from residents of East Palestine, OH, …

Combinatorial Immunotherapy Drives Exhaustion In Tumor Antigen–Specific Cd8+ T Cells Within The Mouse Renal Tumor Microenvironment, Holly R. Stephens, Elizabeth Elkins, Jing Li, Zoey N. Swalley, Henry Nnaemeka Ogbonna, Pia Muri, Zachary Roberts, Francesca R. Dempsey, Md Akkas Ali, Md Hasanul Banna Siam, Richard Kirkman, Jianqing Zhang, Sunil Sudarshan, Daniel J. Tyrrell, Hubert M. Tse, Daniel L. Smith Jr, Lyse A. Norian

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Immunotherapies have greatly improved outcomes for patients with renal cell carcinoma (RCC), yet response rates remain suboptimal and the factors promoting therapy resistance versus sensitivity are incompletely understood. Currently, no preclinical model of orthotopic renal cancer exists that permits evaluation of tumor antigen–specific (TAS) CD8⁺ tumor-infiltrating lymphocytes (TILs). To address this deficiency, we developed a mouse renal cancer model that permits tracking of adoptively transferred TAS CD8⁺ T cells. Renca-LUC tumor cells were transduced to express tumor ERK (tERK), a model antigen expressing a one-amino-acid change from wild-type ERK, resulting in recognition by tERK/H-2K^d-specific DUC Thy1.1 …

Evaluating Sampling Strategies To Monitor Southern Fox Squirrel (Sciurus Niger Niger) Populations, Casey Robin Hitchens

Theses, Dissertations and Capstones

Reliable population monitoring is essential for evaluating wildlife translocations and informing conservation management, yet traditional monitoring approaches are often labor intensive and require prolonged sampling. This study evaluated marking techniques, survey methods, and population estimation approaches for monitoring a translocated population of southern fox squirrels (Sciurus niger niger) at Marine Corps Recruit Depot Parris Island, South Carolina, USA (MCRDPI). From January-June 2023, I conducted six live trapping and camera trapping sessions across five survey sites. I compared the performance of color-coded collars and tail shaves for individual identification and the recapture probability between live traps and camera traps. Logistic mixed-effects …

Nanobubble-Enabled Foam Fractionation To Remove Freshwater Microalgae And Microcystin, Yihan Zhang, Lili Li, Shan Xue, Tsung-Ta David Hsu, Anne C. Hurley, Meiyin S Wu, Xuezhi Zhang, Wen Zhang

Department of Biology Faculty Scholarship and Creative Works

Climate change and water pollution intensify algal blooms in natural lakes and reservoirs, leading to significant water quality challenges. Effectively removing excessive microalgae and algal toxins or micropollutants is essential for improving water quality, preserving aesthetic value, and protecting human health. This study evaluated the use of nanobubble-enabled foam fractionation for the removal of Microcystis aeruginosa and three microcystin congeners (MC-LR, MC-RR, and MC-YR). Foam was generated using air nanobubbles combined with surfactants—cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and chitosan—to enhance foamability and stability. Among the tested surfactants, CTAB exhibited the highest foamability, yielding improved removal performance. When CTAB …

Znf16 Is A Nucleolar-Associated Protein That Regulates Expression Of Rdna And Cancer-Associated Genes, Chelsea L. George, Laura A. Espinoza Quevedo, Jason Paratore, Matthew J. Alcaraz, Arlene P. Levario, Yasir Rahmatallah, Galina V. Glazko, Nathan S. Reyna, Laura Diaz-Martinez

Biology Faculty Scholarship

ZNF16 (also known as HZF1 and KOX9) is a multi-C2H2 zinc finger protein first identified via its expression in human T-cells and shown to have a role in blood cell differentiation. ZNF16 was later shown to be ubiquitously expressed in a variety of fetal and adult tissues, suggesting a broader function. In this study, we confirm the ubiquitous expression of ZNF16 in a variety of cancer and non-cancer cell lines and show that ZNF16 depletion reduces cell viability in all cell lines tested. Furthermore, we show that ZNF16 localizes to the nucleolus in a transcription-dependent manner, interacts with the intergenic …

Impact Of Cross-Client Heterogeneity In Federated Learning For Real-World Plant Disease Classification, Ritesh Janga

All Graduate Theses, Dissertations, and Other Capstone Projects

Deep learning applications are being adopted in agricultural image analysis that include challenges of data privacy and limited institutional data and heterogeneity of different types of architectures. However, Federated Learning is a model that allows collaborative training on data that does not have to be shared among parties. Therefore, Federated Learning is an effective method of collaborative training; however, its comparative effectiveness as compared to individual (local) training on diverse architectures has never been examined in an agricultural context. The objective of this study was to examine Federated Learning for the purpose of crop disease classification on extreme non-IID distributed …

Evaluating The Healthfulness Of The University Of New Hampshire’S Food Environment Using The Healthy Campus Environmental Audit (Hcea), Margo Kamis

UNH URC Open (2026 and after)

Evaluating the Healthfulness of the University of New Hampshire’s Food Environment Using the Healthy Campus Environmental Audit (HCEA)

Soil Organic Carbon And Soil Health Property Responses To Land Management Across Topographic Positions, Mia D. Makovsky

All Graduate Theses, Dissertations, and Other Capstone Projects

uring the last ice age, ice sheets advanced and retreated across the upper Midwest, leaving behind a landscape characterized by poorly drained hummocky topography. Well-aerated soils have formed on uplands and lowlands are dominated by hydric soils. These wetlands and hydric soils have potential to store large quantities of carbon, particularly compared to the well-aerated uplands, but many have been drained and cultivated for decades. Conservation agriculture methods have emerged to protect and conserve soil health from negative effects due to cultivation.The purpose of this study is to determine how agricultural management practices and landscape properties (e.g, topographic position) affect …

Analyzing The Impact Of Sunscreen Pollutants On The Development Of Lytechinus Variegatus, Christina M. Lillenas, Chesney J. Anderson, Ann M. Buchmann

Nebraska Academy of Sciences: Programs and Proceedings

Lytechinus variegatus or the variegated sea urchin is found in shallow, tropical waters. Lytechinus variegatus can be found in sandy reef beds, and sea grass, covered in algae and debris to protect itself from the UV rays and predators. Sea urchins play a crucial role in coral reef ecosystems. Sea urchins follow an omnivore diet, feeding on algae and phytoplankton. In coral reef ecosystems, sea urchins eat unwanted algae, allowing coral reefs to continue growing and thriving. Without the presence of sea urchins in coral reef communities, algae can negatively affect the growth and success of coral reefs. Sunscreen serves …

A Trajectory Of Zostera Marina (Eelgrass) Ecosystem Recovery: Pre- And Post-Hurricane Sandy Degradation In Barnegat Bay, New Jersey, Edgar A. Medina

Theses, Dissertations and Culminating Projects

Hurricane Sandy struck Barnegat Bay, New Jersey in 2012 and obliterated the benthic marine ecosystem with extensive damage to Zostera marina meadows. Previous genetic assessments of Barnegat Bay Z. marina in 2008 revealed low heterozygosity and low connectivity between populations. The great disruption caused by Hurricane Sandy motivated us to investigate the long-term effects of the storm on the genetic condition of the eelgrass populations in the year 2021. The investigation revealed improvements in heterozygosity, inbreeding, and connectivity. These findings drove us to investigate the genetics of Barnegat Bay eelgrass populations in the intervening years of 2013 and 2017 with …