Digital Commons Network^™

Adjunctive Hyperbaric Oxygen Therapy For Chronic Diabetic Foot Ulcer Unresponsive To Standard Care: A Case Report, Ismail Ismail, Sukriyadi Adi, Sitti Rahmatia, Agustan Agustan

BioMedicine

Background: Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus and are often challenging to treat, particularly in patients who fail to respond to standard wound care. Adjunctive therapies such as hyperbaric oxygen therapy (HBOT) have shown potential in promoting wound healing in chronic cases.

Case Presentation: A 66-year-old male with a 10-year history of type 2 diabetes mellitus presented with a chronic, non-healing DFU on the right foot. Despite oral antibiotic therapy and conventional wound management, the ulcer demonstrated progressive necrosis, persistent infection, and tissue exposure. Wound culture identified Klebsiella oxytoca. After targeted antibiotic treatment, the patient …

Neurosurgical Management Of Pineal Lesions: Insights From A Single-Center Study Of 27 Cases, David Montesinos Contreras, Walter Stummer, Maryam Khaleghi Ghadiri

BioMedicine

Introduction: Pineal lesions are rare and surgically challenging due to their deep location, histological diversity, and potential malignancy. The complexity of the pineal region anatomy and the diverse pathological spectrum contribute to the lack of standardized treatment strategies, making appropriate management controversial. Aim: This study aimed to evaluate the surgical outcomes of patients with pineal tumors or cysts and to assess the impact of different surgical approaches, particularly the extent of resection, on progression-free survival. Methods: We conducted a retrospective analysis of 27 patients treated for pineal tumors or cysts at our institution between 2010 and 2020. Data regarding clinical …

A Glimpse Into The Impact Of Physical Activity On Linear Growth In Children And Adolescents Through Activity Restrictions Accompanying The Covid-19 Outbreak, Yi-Chun Lin, Wen-Ling Liao, Chung-Hsing Wang, Fuu-Jen Tsai

BioMedicine

Background: Linear growth in children and adolescents, a key health indicator, is influenced by various factors, including physical activity (PA). The COVID-19 lockdown in Taiwan created a unique opportunity to examine the effect of PA restrictions on growth.

Methods: This retrospective cohort study was conducted at two hospitals in central Taiwan. The study included 72 children and adolescents (26 males, 46 females) who attended a growth clinic. Height and weight measurements were recorded in three stages: pre-lockdown, during lockdown, and post-lockdown. Growth rates were compared across stages using paired t-tests.

Results: The average growth rate was lowest during lockdown (0.0181 …

Assessing The Capabilities Of Ai-Based Large Language Models (Ai-Llms) In Interpreting Histopathological Slides And Scientific Figures: Performance Evaluation Study, Khanisyah Erza Gumilar, Grace Ariani, Priangga A. Wiratama, Rimbun Rimbun, Tri H. Yuliawati, Hong Chen, Ibrahim H. Ibrahim

BioMedicine

Background: Integrating artificial intelligence-based large language models (AI-LLMs) into medical and other scientific domains is increasingly recognized as a tool to support complex tasks, such as interpreting histopathology slides and scientific figures. AI-LLMs can simplify these processes by providing clearer explanations. By improving accessibility and comprehension, AI-LLMs can significantly assist healthcare professionals in diagnosing and therapy determination. Students and the public also find it easier to understand complex scientific concepts and images.

Objectives: This study explores the capability of AI-LLMs in interpreting histopathological slides and scientific images. This study aims to evaluate the performance of AI-LLMs in supporting diagnostics and …

Decyl Caffeate Inhibits The Proliferation Of Human Triple-Negative Breast Cancer Cells, Che-Yi Chao, Woei-Cheang Shyu, Chih-Lung Lin, En-Pei Isabel Chiang, Yueh-Hsiung Kuo, Feng-Yao Tang

BioMedicine

Background: Over recent decades, considerable attention has been directed toward the discovery of novel compounds capable of targeting survival-related signaling networks as therapeutic candidates for triple-negative breast cancer (TNBC). Central to TNBC pathobiology are the Akt/mTOR and MAPK/ERK signaling axes, both contribute to tumor progression and therapeutic resistance. Caffeic acid (CA), a naturally derived phenolic compound with anti-inflammatory activity, has previously been investigated for its anti-cancer potential. Purpose: In the present study, we explored the therapeutic value of newly synthesized CA derivatives in TNBC models using both cellular and animal-based systems. Methods: The anti-tumor efficacy of these CA derivatives was …

The Association Between The Gnb3 Rs5443 C/C Genotype And Obesity Phenotypes In Taiwanese Individuals, Ling-Yi Xiao, Zi-Lun Lai, Yang-Di Su, Szu-Yun Wang, Nia-Jia Zheng, Po- Ren Hsueh

BioMedicine

Background: The prevalence of obesity has increased significantly over the

years, and its health concerns cannot be underestimated. Obesity not only

causes potential mobility limitations in daily life but also increases the risk of

developing cardiovascular diseases, diabetes, cancer and other health

conditions. While an imbalanced diet and lack of exercise are well-known

causes of obesity, genetic patterns also influence its development. Although the GNB3 gene is known to be involved in lipid metabolism and fat cell

differentiation, studies have shown inconsistent associations between a

common single nucleotide polymorphism of GNB3 (c.825C>T, rs5443) and

obesity across different populations. Therefore, …

Chinese Herbal Medicine Treatment And The Association With Long-Term Major Adverse Cardiac Events In Patients With Chronic Kidney Disease: A Propensity-Score Matched Cohort Study, Yuan-Ching Liao, Mei-Yao Wu, Cheng-Li Lin, Chiz-Tzung Chang, Peter Mayer, Hung-Rong Yen

BioMedicine

Background: Cardiovascular disease (CVD) represents the leading cause of

mortality among idividuals with chronic kidney disease (CKD). While Chinese herbal

medicine (CHM) is commonly used by CKD patients in Taiwan, the impact of CHM

use on cardiovascular outcomes in this population remains insufficiently understood.

Aims: This study aimed to evaluate the association between CHM use and the long-

term risk of major adverse cardiac events (MACEs) in patients with CKD.

Methods: Data were obtained from the Taiwan National Health Insurance Research Database to identify patients aged over 20 years with newly diagnosed CKD. A 1:1 propensity score matching was performed …

Increased Dairy Product Consumption Is Associated With Shorter Telomere Length In Buccal Cells Among Normotensive Adults, Hsin-Hwa Tsai, Yang-Di Su, Zi-Lun Lai, Chia-Yu Lin, Shu-Fan Lin, Hsiu-Ching Hsu, Wen-Yuan Lin, Po-Ren Hsueh

BioMedicine

Background: Telomere length (TL) is a biomarker of biological aging and a predictor of age-related diseases. Dietary patterns, including dairy consumption, may influence telomere dynamics, but the evidence remains limited, particularly in Asian populations. This study investigates the association between dairy consumption and relative telomere length (RTL) in Taiwanese adults, with results analyzed by hypertension status.

Methods: A cross-sectional survey was conducted among 259 adults in Taipei, Taiwan. RTL was measured using quantitative PCR from buccal cells. Dietary intake was assessed through self-reported questionnaires, with a focus on dairy frequency and fat content. Multiple linear regression models were used to …

Effectiveness Of A Network Open House Model To Recruit Trainees To Post-Baccalaureate Stem Programs, Sarah Mordan-Mccombs, Scott Takeo Aoki, Lindsay Lewellyn, Sarah Justice, Neetu Tewari, Jorge Cantu, Robert Seiser, Ahmed Lakhani, Jennifer R. Kowalski

Biology Faculty publications

Post-baccalaureate (post-bac) programs can be instrumental in strengthening science training and expanding STEM career opportunities for junior trainees. Many of these sponsored programs are designed to increase research exposure for underrepresented minorities, including African American, Hispanic, Native American, and first-generation college students, among others. Recruiting trainees to post-bac programs can be challenging for reasons including a lack of awareness about available programs. To address this gap, an Open House event was created with the goal of raising awareness and generating interest among potential students for future post-bac programs. Students were recruited from partnering Minority Serving Institutions (MSIs) to attend a …

Determination Of Cortisol Concentrations Using Different Biomarkers In Dairy Goats During Pregnancy, Fatma Mi̇ra Güven, Erkan Pehli̇van

Turkish Journal of Veterinary & Animal Sciences

Pregnancy is considered one of the most physiologically stressful periods in mammals. In the present study, serum and hair cortisol concentrations were measured in nine female Akkeçi goats, including five primiparous (1.5 years old) and four multiparous (3.5 years old), to evaluate chronic stress during pregnancy. Hair samples were collected in October, November, and December following the introduction of bucks to the herd and within the first 12 h postpartum (corresponding to the 1st, 2nd, 3rd, and 7th sampling times). Hair samples were not collected in January, February, or March (the 4th, 5th, and 6th sampling times) due to insufficient …

Effects Of Zinc-Reinforced Clinoptilolite On Hematological Parameters, Igg Levels, And Enzyme Histochemistry In Fattening Lambs, İhsan Kisadere, Muhi̇tti̇n Zengi̇n, Özkan Demi̇rbaş, Mehmet Faruk Aydin

Turkish Journal of Veterinary & Animal Sciences

The study aimed to determine the effects of zinc-reinforced clinoptilolite supplementation to the diet on selected hematological parameters, serum immunoglobulin G (IgG) concentrations, and enzyme histochemical characteristics in fattening lambs. For this purpose, 40 healthy male lambs were randomly allocated into five equal groups: control (C; n = 8), natural clinoptilolite (NC; n = 8), zinc (ZN; n = 8), natural clinoptilolite plus zinc (NCZN; n = 8), and zinc-reinforced clinoptilolite (ZNRC; n = 8). All animals were housed in individual pens, and the supplementation period continued for 42 days. Blood samples were collected before the trial (day 0) and …

Social Roles And Emotional Distress Are Associated With Successful Aging In Adult Childhood Cancer Survivors, Samah Hayek, Kimberly A Miller, Michael E Roth, Michelle A T Hildebrandt, David R Freyer, Joel Milam

Faculty, Staff and Student Publications

Childhood cancer survivors (CCS) face complex long-term health consequences resulting from intensive treatments that may impact their aging trajectory. This study assessed the associations between social role attainment, emotional distress, and indicators of successful aging (SA) among adult CCS, considering treatment intensity. SA, is a multidimensional concept encompassing physical, cognitive, and psychosocial well-being, provides a framework for understanding and optimizing outcomes in this population. This cross-sectional study utilized data from Project Forward, a cohort, of 1,248 adult CCS diagnosed between 1996 and 2010. Participants were identified through the Los Angeles County Cancer Surveillance Program and completed a comprehensive self-report survey …

Cover And Contents

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.

Comparison Of The Mars And Xgboost Algorithms For Predicting Body Weight In Kalahari Red Goats, Louis Thobela Tyasi, Cem Tirink, Kwena Mokoena, Hasan Önder, Uğur Şen, Demet Çanga Boğa, Tolga Tolun, İsmai̇l Gök, Yüksel Aksoy, Lütfi̇ Bayyurt, Ömer Faruk Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Estimating goat body weight from morphometric traits is essential for growth monitoring, health management, and welfare. Accurate weight informs nutrition, clinical interventions, and mating decisions. In this study, the MARS and XGBoost algorithms were used to estimate the live weight of Kalahari Red goats based on body measurements. A dataset containing body length (BL), birth type (single, twin, or triplet), withers height (WH), rump height (RH), and heart girth (HG) information for 200 goats was used in the training and testing of the models. The performances of the models were evaluated with the goodness-of-fit criteria. XGBoost showed a performance of …

Hatchability And Nestling Viability In An Extremely Short-Beaked Pigeon Breed, Türker Savaş, Hakan Erdem, Cemi̇l Tölü

Turkish Journal of Veterinary & Animal Sciences

The selection of hobby animals for breeding by enthusiasts is often based on aesthetic considerations, which can have a negative impact on the animals’ biology. Furthermore, “extreme traits” that deviate from ancestral norms, such as short beaks in pigeons, can be detrimental to the animal’s welfare. Beaks perform many essential functions, such as facilitating feed intake and eggshell breaking. The present study investigates possible hatching problems and the nestling viability of short-beak pigeons through a study of the breeding records of Mısıri (short beak), Baska (medium beak), and Turkish Dönek (normal beak) pigeons, as well as data from one breeding …

Zinc Imbalance And Its Effect On Nutritional Status, Behavior, Gut Microbiota, And Inflammatory Biomarkers In Btbr T+Itpr3tf/J And C57bl/6j Mice: Insights Into Autism Spectrum Disorder, Asma Nasrullah Ahmadani

Thesis/ Dissertation Defenses

Background: Autism spectrum disorder (ASD) has been associated with altered zinc homeostasis and disruptions in the gut microbiota. Zinc is a vital trace mineral involved in multiple biological processes such as inflammatory regulation, intestinal integrity, and neurobehavioral function, and has an important role in influencing microbial activity along the gut–brain axis. Both deficiency and excess of zinc can alter microbial balance. However, their collective effects on behavior, overall physiology, and mineral status in ASD-relevant models are not yet fully understood.

Aim: This comprehensive study explored the effect of dietary zinc variation on body weight, food intake, behavior, gut microbiota composition, …

The Effect Of St. John’S Wort Solution On Climbing Activity Reflecting Depressive-Like Behavior In Drosophila Melanogaster To Model Seasonal Depression In Low Sunlight Exposure, Madison Simon

Journal of the South Carolina Academy of Science

Although depression is a known substantial threat to public health, seasonal affective disorder (SAD) is under-researched. SAD refers to recurrent seasonal patterns of depression. This depressive disorder has few accepted treatments. The purpose of this study was to test whether St. John’s Wort (SJW), a natural supplement with researched antidepressant properties, could be a cheaper alternative with fewer limitations for treating SAD. SJW’s efficacy as a seasonal depression inhibitor was tested through a Drosophila depression-like model using climbing activity to reflect depressive behavior. It was hypothesized that as the concentration of SJW solution increases, the climbing activity of stressed Drosophila …

An Examination Of Trends And Factors Influencing Teen Retention In 4-H Youth Development Program: A Case Study, Bethany R. Corona

LSU Master's Theses

Teen participation in 4-H has declined nationally in recent years, raising concerns about long-term program sustainability and youth engagement. Understanding the factors that influence teens’ decisions to remain involved in 4-H is critical for informing program design and delivery. Guided by the theory of planned behavior, this qualitative study explored teen perceptions of 4-H programming, youth voice, social influences, communication, and barriers to participation. Data were collected through two group interviews with teen 4-H members representing multiple parishes. Interviews were audio recorded, transcribed verbatim, and analyzed using the constant comparative method through a three-phase coding process consisting of open, axial, …

Limitations Of Genetic Testing, Tony N. Jelsma

Faculty Work Comprehensive List

"Knowing about a pathogenic variant may sharpen our awareness of our mortality, but it should not fundamentally change our perspective on life."

Posting about the advantages and disadvantages of genetic screening from In All Things, an online hub that offers insight into maintaining and faithful and orthodox Reformed Christian worldview while fearlessly engaging in every aspect of contemporary life – until all is made new.

Limitations of Genetic Testing

Challenges, Stressors, And Resilience Resources Experienced By Older Black Women In Rural South Carolina Throughout The Covid-19 Pandemic, Arielle N'Diaye, Camryn Garrett, Sarah Wilcox Phd, Bankole Olatosi Ph.D., Xiaoming Li Ph.D., Shan Qiao Ph.D.

Faculty Publications

Background

Resilience has been established as a protective factor against mental health challenges (e.g., anxiety, depression). From an intersectional perspective, the stressors experienced by Black women living in rural communities are likely to have been exacerbated during the COVID-19 pandemic. This study aims to identify the challenges and stressors experienced and the resilience resources available to and relied upon by rural, Black women, during the pandemic.

Methods

Individual, in-depth, structured interviews (n = 24) were conducted among Black women living in rural South Carolina, recruited by local community health workers, from January to April of 2024. All interviews were recorded, …

Concordance Analysis Of Dna And Rna Profiling: The Md Anderson Impact2 Study In Precision Oncology, Stephanie T Schmidt, Mehmet A Baysal, Siqing Fu, David S Hong, Sarina A Piha-Paul, Aung Naing, Jordi Rodon Ahnert, Timothy A Yap, Ecaterina Elena Dumbrava, Jennifer Beck, Funda Meric-Bernstam, Apostolia Maria Tsimberidou

Faculty, Staff and Student Publications

DNA profiling is an established method for cancer treatment selection, while RNA profiling remains investigational. We explored associations between DNA and RNA alterations and between the number of genes with altered expression and overall survival (OS) using patient data from IMPACT2 (NCT02152254), a randomized study evaluating molecular profiling for guiding cancer therapy across tumor types. Molecular profiling, including DNA next-generation sequencing, was performed on all 829 patients in the IMPACT2 study. RNA profiling was performed by Tempus for 253 of 829 patients. We evaluated the concordance between DNA and RNA profiling, analyzed OS in 217 treated patients with …

Risk Stratification Of Low-Dose Cytarabine And Venetoclax In Patients With Aml Ineligible For Intensive Chemotherapy, Andrew H Wei, Panayiotis Panayiotidis, Pau Montesinos, Kamel Laribi, Vladimir Ivanov, Inho Kim, Jan Novak, Rebecca Champion, Walter Fiedler, Maria Pagoni, Julie Bergeron, Stephen B Ting, Jing-Zhou Hou, Takahiro Yamauchi, Jianxiang Wang, Stephen A Strickland, Michael R Savona, Tara L Lin, Anoop Enjeti, Ing Soo Tiong, Sangmin Lee, Gail J Roboz, Relja Popovic, Qi Jiang, Zihuan Liu, Yan Sun, Wellington Mendes, Brenda Chyla, Courtney D Dinardo

Faculty, Staff and Student Publications

Prognostic risk categorization aids treatment selection for patients with acute myeloid leukemia (AML). Although the European LeukemiaNet (ELN) classifications (2017 and 2022) for AML have been used to stratify outcomes for patients receiving intensive chemotherapy, their application to patients receiving less intensive therapy, such as azacitidine plus venetoclax, has been less satisfactory. In response, a 4-gene classifier that stratifies older patients with AML unfit for intensive chemotherapy into those with higher benefit (wild type), intermediate benefit (FLT3-internal tandem duplication [ITD] or NRAS/KRAS mutation), or lower benefit (TP53 mutation) after azacitidine plus venetoclax treatment was developed. We hypothesized that this 4-gene …

Winner's Curse Free Robust Mendelian Randomization With Summary Data, Zhongming Xie, Wanheng Zhang, Jingshen Wang, Chong Wu

Faculty, Staff and Student Publications

In the past decade, the increased availability of genome-wide association studies summary data has popularized Mendelian Randomization (MR) for conducting causal inference. MR analyses, incorporating genetic variants as instrumental variables, are known for their robustness against reverse causation bias and unmeasured confounders. Nevertheless, classical MR analyses using summary data may still produce biased causal effect estimates due to the winner’s curse and pleiotropy issues. To address these two issues and establish valid causal conclusions, we propose a unified robust Mendelian Randomization framework with summary data, which systematically removes the winner’s curse and screens out invalid genetic instruments with pleiotropic effects. …

Eepd1 Evolved A Unique Dna Clamping Dimer Protecting Reversed Replication Forks, Runze Shen, Altaf H Sarker, Yue Chen, Min Liu, Sunetra Roy, Andrew S Arvai, Albino Bacolla, Zamal Ahmed, Panagiotis Katsonis, Michal Hammel, Isao Kuraoka, Miaw-Sheue Tsai, Corydon Irie, Lukas Webb, Olivier Lichtarge, Chi-Lin Tsai, Susan E Tsutakawa, Katharina Schlacher, John A Tainer

Faculty, Staff and Student Publications

Exonuclease/endonuclease/phosphatase (EEP)-fold hydrolases are canonically monomeric phosphodiesterases exemplified by APE1, DNase I, and TDP2 nucleases. While EEP family domain containing protein 1 (EEPD1) acts in DNA stress responses, its proposed nuclease activities are enigmatic. Here, we integrate hybrid structural methods, evolution, biochemistry, cancer genomics, plus molecular and cell biology to define EEPD1 structure, assembly, and function at stalled DNA replication forks. Results imply EEPD1 surprisingly requires both unique EEP domain dimer and distinctive tandem Helix-hairpin-Helix [(HhH)2] domains to clamp double-stranded (ds) DNA at reversed DNA replication forks for fork protection. Small-angle X-ray Scattering (SAXS), crystal, and cryo-EM structures unveil an …

Reliability And Score Agreement Of A Modified Lower Quarter Y-Balance Test As Compared To The Lower Quarter Y-Balance Test In Healthy Adults, Braden L. Lawson, Jordan Morris, Alan Brubaker Rodriguez, Wendy Wheeler

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Introduction: The Y-Balance Test (YBT) is a dynamic balance test assessing range of motion, flexibility, neuromuscular control, and strength. The YBT is used to evaluate individuals for increased injury risk, deficiencies, and progress of rehabilitation. Due to the higher cost associated with materials in a YBT kit, a modified YBT (mYBT) was created using three cloth measuring tapes, and three blocks of wood to help mitigate financial barriers. In previous studies the mYBT has demonstrated adequate agreement of composite scores with the Upper Quarter Y-Balance Test (UQYBT). The purpose of this study was to determine the agreement of composite scores …

The Effect Of Vitamin D Supplementation On Serum 25(Oh)D And Its Association With Stress Fractures In High-Risk, Physically Active Adults: A Critically Appraised Topic, Connor Lewis, Martha Yost, Aric J. Warren

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Clinical Scenario: In collegiate athletic and military populations alike, the repetitive and intense training increase the risk of experiencing a stress fracture, representing a significant time loss and financial cost. Research suggests the use of supplemental vitamin D to prevent the occurrence of stress fractures in high-risk adults. Clinical Question: In high risk, physically active adults, does the effect of vitamin D supplementation on serum 25-hydroxyvitamin D [25(OH)D] concentration effect the occurrence of stress fractures? Clinical Bottom Line: The findings suggest that clinicians should consider the use of supplemental vitamin D as a protective measure against the occurrence of stress …

A Comparison Of Novel Treatment Interventions To Improve Hamstring Range Of Motion, Sadie Buboltz-Dubs, Brittney Dejong, William S. Gear

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

Purpose: Perceived hamstring tightness is a prevalent condition caused by multiple factors, including anterior pelvic tilt and neural tension. Traditional treatments, such as static stretching, do not address these underlying conditions. This study aimed to determine the short-term effectiveness of two neuromuscular treatment techniques for increasing hamstring flexibility compared with a traditional static stretching technique. Methods: Hamstring range of motion was measured using PSLR and AKE pre-and post-intervention for two treatment sessions and again as a single discharge measurement one week after the first treatment session. Three treatments were studied: Primal Reflex Release Technique, Neurodynamic Sliding Technique, Static Stretching, …

Effects Of Functional Ankle Instability On Muscle Activation Latency In Ice Hockey Athletes: A Pilot Study, Nicolas Gonzalez, Tyler A. Wood, Nicholas E. Grahovec

Journal of Sports Medicine and Allied Health Sciences: Official Journal of the Ohio Athletic Trainers Association

This study examined lower extremity muscle activation latency during the push-off phase in ice hockey athletes with and without functional ankle instability (FAI). Ten athletes participated, divided into FAI and control groups based on the Identification of Functional Ankle Instability scale. Participants completed three push-off trials while wearing electromyography sensors on the tibialis anterior, fibularis longus, medial gastrocnemius, and gluteus medius. The FAI group demonstrated a significant increase in muscle activation latency across all muscles tested (p

Role Of Piezo1 Channels Expressed In Pvn In Regulating Sympathetic Nerve Activity And Arterial Blood Pressure In Rats, Yue Chen, Wei Guo, Jichun Wang, Min Wang, Yuying Yang, Gregory Miodonski, Enshe Jiang, Qing Hui Chen, Yi Yang, Renjun Wang

Michigan Tech Publications

Mechanosensitive Piezo1 channels participate in regulating pain sensitivity, insulin secretion, and vascular tension; however, their expression in the autonomic paraventricular nucleus (PVN) and role in modulating sympathetic outflow and cardiovascular function remain unstudied. In this study, unilateral PVN microinjection of the Piezo1 channel blocker Dooku1 (0.1, 1, 10, 100, and 200 pmol) administered to anesthetized male rats increased renal sympathetic nerve activity (RSNA) and mean artery pressure (MAP) in a dose-dependent manner, with maximum increases of 93 ± 30% (p < 0.0001) and 21 ± 5 mmHg (p < 0.0001), respectively, elicited by Dooku1 at 100 pmol. Similarly, PVN microinjection of the peptide Piezo1 channel blocker GsMTx4 (1 nmol) significantly increased RSNA (p < 0.001) and MAP (p < 0.0001). Conversely, PVN-microinjected Piezo1 channel activators Yoda1 (5 nmol) and Jedi2 (5 nmol) did not significantly alter RSNA or MAP. Western blot and qRT-PCR analyses of the hypothalamic PVN showed abundant Piezo1 mRNA and protein expression. Immunofluorescence detection showed that Piezo1 was expressed in pre-sympathetic PVN neurons with axons projecting to the rostral ventrolateral medulla. We conclude that Piezo1 channels expressed in the autonomic PVN neurons play an important role in regulating sympathetic outflow and cardiovascular function.

Cover And Contents

Turkish Journal of Biology

No abstract provided.