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Role Of Metabolic Shifts In Protection From Mutation Damage: Characterizing Mitochondrial Membrane Potential In C. Elegans Gas-1 Mutants, Lauren S. Muñoz-Tremblay

PSU McNair Scholars Online Journal

Many terminal human diseases are caused by mutations affecting mitochondrial functioning. Mitochondria are essential organelles responsible for producing cellular energy, adenosine triphosphate (ATP) via oxidative phosphorylation (OXPHOS) at mitochondrial electron transport chains (ETC). Proper ETC functioning relies on maintenance of the electrochemical gradient essential for energy production, known as mitochondrial membrane potential (ΔψM). The inner mitochondrial membrane is the site of the ETC and is most closely in contact with the enzymatic processes occurring within the mitochondrial matrix. Mutations affecting protein components of the ETC are especially troublesome for organelle health. ETC mutants commonly express altered ΔψM, as well as …

Human Movement Variability And Aging, Nicholas Stergiou, Jenny A. Kent, Denise Mcgrath

Journal Articles

An optimal level of variability enables us to interact adaptively and safely to a continuously changing environment, where often our movements must be adjusted in a matter of milliseconds. A large body of research exists that demonstrates natural variability in healthy gait (along with variability in other, healthy biological signals such as heart rate) and a loss of this variability in aging and injury, as well as in a variety of neurodegenerative and physiological disorders. We submit that this field of research is now in pressing need of an innovative “next step” that goes beyond the many descriptive studies that …

Effects Of The Developmental Environment On Oxidative Damage And Antioxidants In Red-Eared Slider Turtle ( Trachemys Scripta Elegans ) Hatchlings, Lisa Allison Treidel

Theses and Dissertations

Developmental environments influence many individual phenotypes. However, currently we have a limited understanding of how the developmental environment influences oxidative stress resistance phenotypes. Oxidative stress is defined as a physiological state during which the production of harmful free radicals exceeds the protective capabilities of antioxidants. Variations among adults in susceptibility to oxidative stress can have important consequences for life history strategies and fitness related traits. Our work was aimed at providing insight in to the role that the developmental environment plays on oxidative stress phenotypes in the model oviparous reptile the red-eared slider turtle (Trachemys scripta elegans). Here, we conducted …

Hearing Sensitivity And The Effect Of Sound Exposure On The Axolotl (Ambystoma Mexicanum), Amy K. Fehrenbach

Masters Theses & Specialist Projects

The axolotl (Ambystoma mexicanum) has been used as a model organism for studying development, genetics, and regeneration. Although the sensory hair cells of the lateral line of this species have been shown to be able to regenerate, it is not known whether this also occurs in the inner ear. In fact, little is known about the hearing capabilities of the axolotl or other salamander species. I recorded auditory evoked potentials (AEPs) of six axolotls at eleven frequencies (0.1, 0.25, 0.4, 0.6, 0.8, 1, 1.5, 2, 3, 4, and 6 kHz) in order to produce baseline audiograms of underwater pressure sensitivity. …

The Effect Of Balance-Based Torso-Weighting On Mobility, Gait, Balance, Postural Control, And Falls Efficacy In Mobility Limited Older Adults, Jennifer L. Vincenzo

Graduate Theses and Dissertations

Exercise is a known intervention to prevent fall risk among older adults; however, adherence is poor. Therefore, it is of interest to determine if other interventions improve function and decrease fall risk among older adults. Balance-Based Torso-Weighting (BBTW) is a non-exercise intervention that improves functional measures among adults with multiple sclerosis, yet the effectiveness of BBTW has not been assessed among older adults without progressive neurological disorders. We conducted a double-blind, randomized study to analyze the effect of BBTW on functional measures and falls efficacy among community-dwelling, mobility limited older adults after 5 days of wearing BalanceWear® for 4 hours …

The Effect Of Physical Activity Levels On Hydration Markers In Non-Athletic Free-Living Individuals, Mikell Lucas Hammer

Graduate Theses and Dissertations

Higher levels of physical activity requires increased fluid intake due to increased water losses via sweating. PURPOSE: To determine the effect of physical activity on hydration status and water intake. METHODS: This study involved 8 visits to the Human Performance Laboratory over 22 days. Body weight and urine measurements were taken every visit. Physical activity was assessed by the International Physical Activity Questionnaire (IPAQ), water intake by the Water Frequency Questionnaire (WFQ), and hydration status by urine osmolality (UOsm), urine specific gravity (USG), and urine color (UC). From the IPAQ subjects were classified as low, moderate and high physical activity …

Thrombospondin-1 Decreases No-Mediated Vasodilation In Coronary Arterioles In Advancing Age., Grant Mckenzie

College of Arts & Sciences Senior Theses

No abstract provided.

Temporal Structure Of Support Surface Translations Drive The Temporal Structure Of Postural Control During Standing, Troy J. Rand, Sara A. Myers, Anastasia Kyvelidou, Mukul Mukherjee

Journal Articles

A healthy biological system is characterized by a temporal structure that exhibits fractal properties and is highly complex. Unhealthy systems demonstrate lowered complexity and either greater or less predictability in the temporal structure of a time series. The purpose of this research was to determine if support surface translations with different temporal structures would affect the temporal structure of the center of pressure (COP) signal. Eight healthy young participants stood on a force platform that was translated in the anteroposterior direction for input conditions of varying complexity: white noise, pink noise, brown noise, and sine wave. Detrended fluctuation analysis was …

Assessment Of Stabilization And Muscle Recruitment In Amputees Following Sit-To-Stand Transfer, Elizabeth Terran Jones

Health, Human Performance and Recreation Undergraduate Honors Theses

Standing from a seated position is a task of daily living that is essential for independence relying heavily on balance and lower-extremity muscle strength and coordination (Whitney et al., 2005). With age, many once healthy individuals lose this ability and are institutionalized or find themselves reliant on others for care due to the development of balance disorders or loss of muscle mass. The majority of research present today on transitional movements affected by balance disorders, such as the sit-to-stand transition, has been focused on the elderly population. A correlation has been established between the Sit-To-Stand Test and factors such as …

Examining The Relationship Between Neurocognitive Performance And Functional Fitness Levels Among Older Adults, Kristen J. Holmes

Health, Human Performance and Recreation Undergraduate Honors Theses

The goal of this thesis was to examine the relationship between neurocognitive performance and functional fitness levels among older adults. This is important because the geriatric population is the fastest growing demographic in the United States (USDHHS, CDC & Prevention, 2012). By 2030, the older adult population is expected to double. With the cumulative health effects associated with aging and pathological disease, adults typically experience a normal decline in cognitive function termed age-associated memory impairment (AAMI) (Kramer, Colcombe, McAuley, Scalf, & Erickson, 2005; O’Brien, 1999). It is believed to be a non-progressive disorder. Mild cognitive impairment (MCI), on the other …

Validation Of The Tendo Weightlifting Analyzer As A Method To Assess Muscular Power, Emily Janssen

Health, Human Performance and Recreation Undergraduate Honors Theses

Power is a strong indicator of physical fitness and functional status of older adults. The Tendo Weightlifting Analyzer is a safe, cost-effective method to measure power. The goal was to validate the Tendo as a measure of muscular power during a chair stand task. It was hypothesized that the Tendo would be a valid measure. This study was a cross-sectional, correlational study with the subjects serving as their own control. All testing took place in the Human Performance Lab at the University of Arkansas. A total of 59 college-aged students from 18 to 30 years of age volunteered. Subjects were …

Additional Sex Combs-Like 2 (Asxl2): A Psychological Stress-Related Gene In A Chicken Stress Model, Emily Sandra Jacobson

Chemistry & Biochemistry Undergraduate Honors Theses

Chronic stress is a problem not only in terms of its psychological effects but also due to the harmful physiological changes induced in the individual. Although chronic stress is recognized as detrimental and rampant throughout society, the stress system itself remains poorly understood in terms of comprehending which genes are involved in regulating and carrying out stress-induced signals. In a previous study, 57 candidate genes in the stress pathway had been identified based on the presence of stress-associated single nucleotide polymorphisms (SNPs) and differential expression from microarray data. The objective of this study was to investigate Additional Sex Combs-Like 2 …

Metabolic Theory Explains Latitudinal Variation In Common Carp Populations And Predicts Responses To Climate Change, Michael J. Weber, Michael L. Brown, David H. Wahl, Daniel E. Shoup

Natural Resource Management Faculty Publications

Climate change is expected to alter temperature regimes experienced by fishes, which may also alter life history traits. However, predicting population-level responses to climate change has been difficult. Metabolic theory of ecology has been developed to explain how metabolism controls a variety of ecological processes, including life history attributes. Thus, this theory may be a useful tool for predicting fish population responses to climate change. To understand how climate change may alter freshwater fish life history, we measured population characteristics (e.g., recruitment, growth, body size, and mortality) of 21 North American common carp Cyprinus carpio populations spanning a latitudinal gradient …

Biomechanical Analyses Of Stair-Climbing While Dual-Tasking, Srikant Vallabhajosula, Chi Wei Tan, Mukul Mukherjee, Austin Davidson, Nicholas Stergiou

Journal Articles

Stair-climbing while doing a concurrent task like talking or holding an object is a common activity of daily living which poses high risk for falls. While biomechanical analyses of overground walking during dual-tasking have been studied extensively, little is known on the biomechanics of stair-climbing while dual-tasking. We sought to determine the impact of performing a concurrent cognitive or motor task during stair-climbing. We hypothesized that a concurrent cognitive task will have a greater impact on stair climbing performance compared to a concurrent motor task and that this impact will be greater on a higher-level step. Ten healthy young adults …

H-Index Is Important For Postural Control For People With Impaired Foot Sole Sensation, Shuqi Zhang, Brad Manor, Li Li

Health Sciences & Kinesiology: Faculty Publications

Introduction: People with Peripheral Neuropathy (PN), especially those with impaired sensory inputs through the small-afferent fiber (type II afferent fibers) reflex loop (SAF), might depend more on the large-afferent fiber (type I afferent fibers) reflex loop (LAF) for postural control.

Purpose: To examine whether the function of the LAF reflex loop, reflected by the H-reflex and ankle joint proprioception, influences postural control when the SAF reflex loop is impaired, as indicated by reduced foot sole cutaneous sensation.

Methods: Thirteen participants (8 women, 5 men) diagnosed with PN and 12 age-matched controls (7 women, 5 men) completed the testing protocol. Measures …

Latitudinal Variation In Lifetime Survival And Reproduction In A Burying Beetle, Clinton T. Laidlaw

Theses and Dissertations

Temperature variation experienced across a latitudinal range is tied to changes in lifespan and life history across multiple taxa. Two patterns of adaptation to latitudinal temperature variation have been documented – counter-gradient (or co-gradient) variation, and local adaptation. To determine how natural selection has shaped life history variation in a burying beetle, Nicrophorus orbicollis, we quantified lifetime patterns of reproduction in two populations that represent the geographic and temperature extremes occupied by the species. Lifetime reproduction was quantified at two temperatures that represented conditions typical for each population. Burying beetles from different extremes of their geographic range show considerable variation …

The Association Of Cognitive Endophenotypes And Risky Single Nucleotide Polymorphisms Of Alzheimer's Disease Within The Alzheimer's Disease Neuroimaging Initiative (Adni) Database, Kyle Joseph Jennette

USF Tampa Graduate Theses and Dissertations

Objective: The purpose of this study was to assess the influence of three single nucleotide polymorphisms (SNP) previously associated with Alzheimer's disease on specific domains of cognition, when controlling for Apolipoprotein E gene (APOE), in a sample of individuals with Alzheimer's disease. Methods: The data were drawn from the Alzheimer's Disease Neuroimaging Initiative database, a comprehensive, longitudinal database of controls, persons with mild cognitive impairment, and persons with mild Alzheimer's disease. Each subject has a full neuropsychological assessment, neuroimaging, genetic sequencing, and physical evaluation. For the purposes of this study, individuals were selected based on the presence of the three …

A Qualitative Assessment Of Eating Behaviors In Adults With Autism, Samantha Barbier

Theses and Dissertations

Rationale: Diagnosis of autism has increased ten-fold in the last 40 years, and adults with autism remain an underrepresented population in research. Purpose: To qualitatively explore the relationship between eating behaviors and autism using a questionnaire and interviews with adults diagnosed with autism spectrum disorder. Methods: Four males aged 22-27 were interviewed with their mothers present and completed the Swedish Eating Assessment for Autism Spectrum Disorders (SWEAA). Interviews were analyzed through the process of open coding, and the questionnaires were analyzed to determine consistent findings between the interview and the SWEAA questionnaire. Major Findings: Participants generally recognized hunger and satiety …

Walking Before And During A Sea Voyage, Chih-Hui Chang, Nicholas Stergiou, Jeffrey P. Kaipust, Eric Haaland, Yi Wang, Fu-Chen Chen, Thomas A. Stoffregen

Journal Articles

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Abstract

Stationary and moving surfaces impose different constraints on walking. In this study we investigated within-participants differences between walking on a ship before (at the dock) and during (at sea) a sea voyage. Four individuals participated in the study. While on the ship they wore a tri-axial accelerometer (ActiGraph GT3X+; ActiGraph LLC, Pensacola, FL) on their waists. Activity data were sampled at 30 Hz. Data were collected on the day before the voyage began and on several days at sea. The number of steps per day was greater at the dock than at sea. …

Postnatal Growth Rates Covary Weakly With Embryonic Development Rates And Do Not Explain Adult Mortality Probability Among Songbirds On Four Continents, Thomas E. Martin, Oteyza C. Oteyza, Adam E. Mitchell, Ahva L. Potticary, Penn Lloyd

Wildlife Biology Faculty Publications

Growth and development rates may result from genetic programming of intrinsic processes that yield correlated rates between life stages. These intrinsic rates are thought to affect adult mortality probability and longevity. However, if proximate extrinsic factors (e.g., temperature, food) influence development rates differently between stages and yield low covariance between stages, then development rates may not explain adult morality probability. We examined these issues based on study of 90 songbird species on four continents to capture the diverse life-history strategies observed across geographic space. The length of the embryonic period explained little variation (ca. 13%) in nestling periods and growth …

Dairy Consumption And Insulin Resistance: The Role Of Body Fat, Physical Activity, And Energy Intake, Larry A. Tucker, Andrea Erickson, James D. Lecheminant, Bruce W. Bailey

Faculty Publications

The relationship between dairy consumption and insulin resistance was ascertained in 272 middle-aged, nondiabetic women using a cross-sectional design. Participants kept 7-day, weighed food records to report their diets, including dairy intake. Insulin resistance was assessed using the homeostatic model assessment (HOMA). The Bod Pod was used to measure body fat percentage, and accelerometry for 7 days was used to objectively index physical activity. Regression analysis was used to determine the extent to which mean HOMA levels differed across low, moderate, and high dairy intake categories. Results showed that women in the highest quartile of dairy consumption had significantly greater …

Progressive Resistance Exercise With Eccentric Loading For The Management Of Knee Osteoarthritis., Haniel J. Hernandez, Valerie Mcintosh, Azadeh Leland, Michael O. Harris-Love

Exercise and Nutrition Sciences Faculty Publications

INTRODUCTION: The patient was a 58-year-old African-American male with radiographic evidence of bilateral knee osteoarthritis (OA). He participated in a standardized 12-week eccentric strengthening program within a Veterans Affairs (VA) medical center.

BACKGROUND: The use of an eccentric training paradigm may prove to be beneficial for older adults with knee OA since eccentric muscle actions are involved in the energy absorption at the knee joint during gait and controlled movement during stair descent. Furthermore, in comparison to standard muscle actions, eccentric muscle actions result in higher torque generation and a lower rate of oxygen consumption at a given level of …

Body Size And Age In Three Populations Of The Northern Banded Newt Ommatotriton Ophryticus (Berthold, 1846) From Turkey, Çi̇çek Özcan, Nazan Üzüm

Turkish Journal of Zoology

We measured body size (snout-vent length, SVL) for three populations of Ommatotriton ophryticus newts in Turkey and estimated age using the skeletochronological method. Age was determined by counting the lines of arrested growth in cross-sections taken from phalanges. SVL was similar among populations and ranged between 58.41 and 85.78 mm in males and 50.33 and 71.56 mm in females. Males were significantly larger than females in all populations. Age at maturity was 4-6 years in males and 5-6 years in females. The average age of males and females did not differ significantly. Maximum age ranged from 9 to 12 years …

Relationships Between Fish Length And Otolith Size For Five Cyprinid Speciesfrom Lake Ladik, Samsun, Turkey, Savaş Yilmaz, Okan Yazicioğlu, Ramazan Yazici, Nazmi̇ Polat

Turkish Journal of Zoology

The relationships between size (length and width) of the lagenar (asteriscus) and the utricular (lapillus) otoliths and body length for five cyprinid fish species, freshwater bream Abramis brama, white bream Blicca bjoerkna, Prussian carp Carassius gibelio, brond-snout Chondrostoma regium, and rudd Scardinius erythrophthalmus, collected from Lake Ladik from November 2009 through October 2010, are presented. The right and left side measurements of otoliths were pooled in all cases except asteriscus length in Prussian carp. Nonlinear and linear functions provided the best fit for 80% and 20% of all species, respectively. All relationships were highly significant (P < 0.001, R2 > 0.71) and the mean …

Age Estimation Of Anatololacerta Anatolica (Werner, 1902) In The Vicinity Of Çanakkale By Skeletochronology, Batuhan Yaman Yakin, Cemal Varol Tok

Turkish Journal of Zoology

In this study, age estimation using skeletochronology was done in 43 specimens (6 males, 29 females, 8 juv.) of Anatololacerta anatolica living in the vicinity of Çanakkale. When the cross-sections taken from phalanges were examined, the median age for the Çanakkale population was 4 years. The maximum age was calculated as 10 years for female individuals, and the maximum snout-vent length (SVL) of female individuals was measured as 74.18 mm. The mean SVL was 57.39 (SD = 4.6) mm for males and 63.62 (SD = 8.62) mm for females. The age at sexual maturity was determined as 3 years for …

The Actn3 R577x Polymorphism Is Associated With Cardiometabolic Fitness In Healthy Young Adults., Chelsea L Deschamps, Kimberly E Connors, Matthias S Klein, Virginia L Johnsen, Jane Shearer, Hans J Vogel, Joseph M. Devaney, Heather Gordish-Dressman, Gina M Many, Whitney Barfield, Eric P. Hoffman, William E Kraus, Dustin S Hittel

Genomics and Precision Medicine Faculty Publications

Homozygosity for a premature stop codon (X) in the ACTN3 “sprinter” gene is common in humans despite the fact that it reduces muscle size, strength and power. Because of the close relationship between skeletal muscle function and cardiometabolic health we examined the influence of ACTN3 R577X polymorphism over cardiovascular and metabolic characteristics of young adults (n = 98 males, n = 102 females; 23 ± 4.2 years) from our Assessing Inherent Markers for Metabolic syndrome in the Young (AIMMY) study. Both males and females with the RR vs XX genotype achieved higher mean VO2 peak scores (47.8 ± 1.5 vs …

"We Are All One Together": Peer Educators' Views About Falls Prevention Education For Community-Dwelling Older Adults - A Qualitative Study, Linda Khong, Fiona Farringdon, Keith D. Hill, Anne-Marie Hill

Health Sciences Papers and Journal Articles

Background: Falls are common in older people. Despite strong evidence for effective falls prevention strategies, there appears to be limited translation of these strategies from research to clinical practice. Use of peers in delivering falls prevention education messages has been proposed to improve uptake of falls prevention strategies and facilitate translation to practice. Volunteer peer educators often deliver educational presentations on falls prevention to community-dwelling older adults. However, research evaluating the effectiveness of peer-led education approaches in falls prevention has been limited and no known study has evaluated such a program from the perspective of peer educators involved in delivering …

The Relationships Among Bmi, Waist Circumference, Weight Loss And Health Indicators, John R. Rupp

Theses and Dissertations--Nutrition and Food Systems

Current health recommendations encourage weight loss for overweight and obese individuals. However, the importance of weight to health is still unclear. The purpose of this study was to determine the impact of weight loss on overall health in a sample of adults who have completed a 10 or 12 week weight loss intervention. The study sample included males and females (n =99) between the ages of 27 -64 years that had a BMI of 29-45 kg/m². The study design is a secondary data analysis of data from two different behavioral weight loss interventions. Both interventions prescribed a reduced calorie diet, …

The Effects Of Hibernation On The Hemostatic Properties Of The American Bullfrog, Rana Catesbeiana, Kiana Cullinan

Honors Program Theses

It is known that hibernating animals display a drastic reduction in metabolic rate, accompanied by reduced heart rate and rate of blood flow through vessels. Sluggish blood flow increases the risk for developing clots, yet it has been observed that hibernators conclusively display elongated clotting times. This observed impairment may be caused by the decrease in body temperature, due to a negative impact on the functioning of the enzymes involved in coagulation. Some hibernating endotherms, or “warm-blooded” animals, employ biological mechanisms as preventative means to prevent clotting during hibernation, such as the down-regulation of certain clotting factors or the production …

A Therapeutic Approach For Senile Dementias: Neuroangiogenesis, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Alzheimer's disease (AD) and related senile dementias (SDs) represent a growing medical and economic crisis in this country. Apart from cautioning persons about risk factors, no practical, effective therapy is currently available. Much of the recent research in AD has been based on the amyloid cascade theory. Another approach assumes a vascular basis for SDs. This paper presents evidence from a score of studies that cerebral capillary density (CCD) declines during old age in animals and people as well as in AD. Neuroangiogenic (NAG) factors initiate and maintain capillaries in the brain. Thus a waning level of these factors and …