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Volume 16, Maggie Duncan, Madeline Little, Alicia Hoffman, Megan Livesay, Gabrielle Quaresma, Serenity Allen, Laina Pfountz, Ainslie Allred, Sabrina Robles, Nicholas J. Duellman, Trinity L. Deguzman, Melissa H. Savage, Margaret Dudley, Jocelyn Escobar, Olivia Hildreth, Olivia Hopkins, Benjamin Gettier, Lee Kassay, Jade Riddle, Ashley Seiders

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Amorette Barber, Director, Office of Student Research

From the Editor Dr. Hannah Dudley-Shotwell

Artist’s Statement Maggie Duncan

On Mentoring Dr. Lee Millar Bidwell

The Hujum Campaign in Uzbekistan and its Consequences by Madeline Little

Wet Cupping Compared to Dry Needling for Treatment of Patients with Low Back Pain: A Critically Appraised Topic by Alicia Hoffman and Megan Livesay

Optimization of eDNA Air Sampling Via 3D Printed Fan by Gabrielle Quaresma

Beyond the Classroom: A Qualitative Study of Teacher Attrition and Retention by Serenity Allen and Laina Pfountz

The Treatment of Subacromial Impingement Syndrome with Platelet-Rich plasma Injections Verses …

Discovery, Purification And Annotation Of Four Novel Mycobacteriophages In New England, Mason Krawczynski

Chemistry & Biochemistry Student Scholarship

Mason Krawczynski ’26, Biology and Psychology major

Faculty mentor: Dr. Kathleen Cornely, Chemistry and Biochemistry

Mycobacteriophages are viruses that infect bacteria of the Mycobacterium genus. Several thousand mycobacteriophages have been discovered, isolated, and characterized to advance the current understanding of their genomic diversity. The mycobacterium phage selection proves valuable, as they have a broad host range and potential for therapeutic application to treat pathogenic and antibiotic-resistant mycobacteria, such as Mycobacterium tuberculosis, the causative agent of tuberculosis infection. Kraw was isolated from a soil sample collected in Oaks Bluff, Massachusetts, on the island of Martha’s Vineyard in July 2023. FairyPath, Calvinny, …

Tools For Harnessing Atropisomerism In 1-Aryl-Β-Carbolines, Cristina Díaz, Malaquias Loiza, Josef Crenshaw, Brian Ladino, Elizabeth Perda, Alma Martinez, Joseph Mazzucca, John Stathoulopoulos

Chemistry & Biochemistry Student Scholarship

Cristina Díaz ’25, Chemistry major

Malaquias Loiza ’26, Biochemistry major

Josef Crenshaw ’28, Biochemistry major

Brian Ladino ’28, Biochemistry major

Elizabeth Perda ’26, Biochemistry major

Alma Martinez ’26, Biochemistry major

Joseph Mazzucca ’25, Biochemistry major

John Stathoulopoulos ’26, Chemistry major

Faculty mentor: Dr. Seann Mulcahy, Chemistry and Biochemistry

Axially chiral molecules with high barriers to rotation are becoming increasingly more prominent in drug discovery, chemical biology, and materials science. We have shown that 1-aryl-β-carbolines exhibit high configurationally stability, with barriers to rotation greater than 30 kcal/mol and have recently disclosed an asymmetric synthesis of these molecules. In this presentation, we …

Discovery, Purification And Annotation Of A Novel Mycobacteriophage Discovered On The Providence College Campus, Jenna Greene

Chemistry & Biochemistry Student Scholarship

Jenna Greene ’26, Biochemistry major

Faculty mentor: Dr. Kathleen Cornely, Chemistry and Biochemistry

Bacteriophages—viruses that infect bacteria—offer a promising alternative to antibiotics in the face of rising antibiotic resistance. In this project, I isolated a novel phage from a soil sample collected in front of Raymond Dining Hall at Providence College. Using Mycobacterium smegmatis as the host, the sample underwent multiple rounds of purification until a single phage, named GMonster (a nod to my family nickname, “Greene Monster”), was isolated. GMonster was submitted to the PhagesDB database and confirmed through sequencing to belong to the A1 cluster—the first of its …

Exploring An Electron-Rich Iron Tricarbonyl Complex Containing An Α-Diimine Ligand, Joseph Maher

Chemistry & Biochemistry Student Scholarship

Joseph Maher ’25, Chemistry major

Faculty mentor: Dr. Maria Carroll, Chemistry and Biochemistry

The combustion of fossil fuels increases atmospheric CO2, making hydrogen a promising cleaner energy alternative. Pt has been able to facilitate this reaction efficiently, however, it is expensive and scarce. Fe has shown promise in facilitating 2-electron transfers when bound to redox-active ligands. Reacting the redox-active CyDABMe with benzylidene acetone iron tricarbonyl (bdaFe(CO)3) forms Fe[CyDABMe](CO)3, with vCO values ~10 cm-1 lower than FeArDABMe(CO)3, indicating increased electron density. The reduction potential of CyDABMe was probed using Zn[CyDABMe]Cl2, showing a reduction at 200 mV more negative than Zn[ArDABMe]Cl2. X-ray …

Anxiety- And Compulsivity-Linked Differences In Reinforcement Learning In A Choose-Your-Own-Adventure Game, Tessa Medeiros

Neuroscience Program Student Scholarship

Tessa Medeiros ’26, Biology & Psychology major

Faculty mentor: Dr. Andra Geana, Psychology and Neuroscience

Reinforcement learning (RL) mathematically describes how agents learn to maximize the reward of their environment by understanding and comparing their choice options. This project investigates how anxiety and compulsivity impact the selection of these options and to what extent a text-based, narrative (rather than a game-like environment) changes RL processes in humans.

Using Enviromental Dna To Detect And Quantify Atlantic Silversides In Rhode Island, Owen Finnegan

Biology Student Scholarship

Owen Finnegan ’25, Environmental Biology major

Faculty mentor: Dr. Jeffrey Markert, Biology

Here we test whether eDNA concentrations consistently reflect the abundance of fish in a given area. We compare physical catches to the levels of eDNA in the water using quantitative PCR (qPCR) at three sites in Rhode Island. A native and plentiful species like Atlantic Silversides is the perfect organism to for calibrating our methods as recorded catches of Atlantic Silversides from our collections range from 0-1500+ individuals.

The Effect Of Salinity On Mangrove Growth In The Florida Everglades, Olivia Macaluso

Biology Student Scholarship

Mangrove forests are highly productive ecosystems that provide benefits to humans, such as storm protection, in the intertidal regions of tropic and subtropic climates. Mangroves grow primarily in shallow brackish water where fresh and salty water sources meet, but frequent hurricanes mean that once stable brackish environments are vulnerable to fluctuations in salinity levels. A previous study on the effect of salinity on mangroves found a negative relationship between salinity increase and root biomass growth. However, little research has gone into the effects of salinity changes on mangrove growth and productivity. Here, I used data from the Wetland and Aquatic …

Environmental Racism In Ri: The Presence Of Toxic Waste Sites In Low Income And Minority Communities (19), Julienne Walsh

Biology Student Scholarship

Environmental racism refers to the disproportionate impact of environmental hazards on communities of color and low-income populations. Across the U.S., studies have shown that polluting facilities are more likely to be in or around communities with higher percentages of non-white residents or lower socioeconomic status. Scholarship regarding these patterns in small states like Rhode Island is relatively limited. By analyzing how these sites are spatially distributed in relation to racial and economic demographics, this research aims to contribute to a deeper understanding of local environmental inequities. I hypothesize that if you live in a low-income or more diverse town, national …

Microneedles Potentiate Skin Cell Rejuvenation Via Modulation Of Mitochondria And Epigenetic Activities, Alexandra Delano, Angelika Kartsagoulis

Biology Student Scholarship

Alexandra Delano ’25, Biology major

Angelika Kartsagoulis ’25, Biology major

Faculty Mentor: Dr. Yinsheng Wan, Biology

Characterizing The Functional Diversity Of Southern Ocean Chordates, Lillian Stern

Biology Student Scholarship

Lillian Stern ’26, Biology major

Faculty mentor: Dr. Roxanne Banker, Biology

Biological invasions are… Invasion events can alter ecosystem properties like species interactions, trophic rank, and abundance. Current scientific research struggles to quantify the effects of biotic invasions effect on ecosystem structure and function. Measuring functional diversity, or the diversity traits such as feeding and living habits, in an ecosystem can provide important information about how organisms and the environment respond to environmental change. Here, we investigate functional diversity of the Chordates in a marine Southern Ocean community that has already been affected by biological invasion. By characterizing how these …

Finding Patterns In Disturbance: Modeling Ecosystem Recovery Using A Machine Learning Approach, Paden D. Allsup

Theses and Dissertations

Some of the most visible effects of Anthropogenic climate change are shifts in resource (water) availability, and disturbance regimes across the world. Ecosystems across the world are now being forced to adapt to amplified disturbances, or in some cases, completely new disturbance types. These shifts have major implications for ecosystem stability and functioning, and more broadly for nutrient cycling across the world. In the western USA, wildfire is playing a larger and more dramatic role than before, altering vegetation communities, landscape stability, and soil and stream chemistry. While fire has always played an important role in semi-arid ecosystems, the scale …

Assessing Innovations In Wildlife Monitoring: Estimating Abundance And Inferring Parturition Of Ungulates With Technology, Elicia Cotcher

Theses and Dissertations

Enhancements in technology are creating opportunities for novel methods of wildlife monitoring. Traditional methods of wildlife monitoring are often costly or time-consuming, and new technological advancements such as remote cameras and GPS collars are creating opportunities for exploration as these technologies become more accessible and reliable. We sought to assess the efficacy of two novel methods of ungulate monitoring. The first is an abundance estimation method using remote cameras termed Instantaneous Sampling (IS). IS seeks to estimate abundance using time lapsed remote cameras on a population of unmarked individuals. Our goal was to compare IS estimations against the true observed …

Dna And Bioinformatics Tools For Estimating Biodiversity In The Narragansett Bay, Emma Barry

Biology Student Scholarship

Emma Barry ’25, Environmental Biology major

Faculty mentor: Dr. Jeffrey Markert, Biology

Effects Of Novel Fire Regimes On Plant And Animal Communities, Ryan Pienaar

Theses and Dissertations

Human actions are threatening global biodiversity through changes to ecosystems to which species are not well adapted. One of the main pathways by which humans affect change is through altered disturbance regimes. This includes changes in the size, frequency, and severity of wildfires. The impact of wildfires on native plant and animal communities varies across ecosystems as species in a community have adapted relatively predictable fire patterns over time. Changing fire patterns alters successional dynamics that can favor certain species over others and thus have a negative impact on biodiversity. This dissertation evaluates responses of different plant and animal species …

Thinking Beyond Association Tests: Using Mathematical Models To Identify Genes Influencing Exceptional Longevity, Sandeep Acharya

McKelvey School of Engineering Graduate Student Theses & Dissertations

The main goal in the genetic study of trait biology is to identify genetic factors, such as variants and genes, that causally influence traits and to understand the downstream mechanisms through which they exert their effects. This dissertation improves our understanding of trait biology by building on widely applied omics-wide association studies (omics-WAS), such as GWAS and TWAS, and proposing methods that (1) combine association statistics from multiple omics modalities and (2) systematically incorporate prior knowledge from biological networks and functional annotations with omics-WAS summary statistics. First, we apply meta-analysis to integrate gene-level p-values from GWAS, TWAS, and rare-variant analyses …

Exploring Ddr2’S Role In Caf Extracellular Matrix Production And Tumor Progression, Favour Ayomide Akinjiyan

Arts & Sciences Graduate Student Theses and Dissertations

Ovarian cancer has poor survival outcomes particularly for advanced stage, metastatic disease. Metastasis is promoted by interactions of stromal cells, such as cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME), with tumor cells. CAFs play a key role in tumor progression by remodeling the TME and extracellular matrix (ECM) to result in a more permissive environment for tumor progression. It has been shown that fibroblasts, in particular myofibroblasts, utilize metabolism to support ECM remodeling. However, the intricate mechanisms by which CAFs support collagen production and tumor progression are poorly understood. In this study, we show that the fibrillar collagen receptor, …

Retrospective Analysis Of Cement Extravasation Rates In Vertebroplasty, Kyphoplasty, And Bone Tumor Radiofrequency Ablation, Soun Sheen, Prit Hasan, Xiaowen Sun, Jian Wang, Claudio Tatsui, Saba Javed

Faculty, Staff and Student Publications

Background: Percutaneous vertebral augmentation techniques, including vertebroplasty, kyphoplasty, and bone tumor radiofrequency ablation (BT-RFA), are commonly used to treat painful vertebral compression fractures (VCFs). While generally safe and effective, they carry risks, including cement extravasation, which can lead to pulmonary embolism or spinal cord compression. This study aims to compare the rate of cement extravasation across different vertebral augmentation techniques and identify potential risk factors.

Methods: A retrospective cohort study was conducted at a comprehensive cancer center on 1002 procedure encounters in 888 patients who underwent vertebral augmentation for painful VCFs. Data were collected on patient demographics, fracture pathology, procedure …

Development And Validation Of A Dynamic Real-Time Risk Prediction Model For Intensive Care Units Patients Based On Longitudinal Irregular Data: Multicenter Retrospective Study, Lan Lan, Xiaobo Zhou, Xiaoyan Yang, Shixin Huang

Faculty, Staff and Student Publications

Background: Timely and accurate prediction of short-term mortality is critical in intensive care units (ICUs), where patients' conditions change rapidly. Traditional scoring systems, such as the Simplified Acute Physiology Score and Acute Physiology and Chronic Health Evaluation, rely on static variables collected within the first 24 hours of admission and do not account for continuously evolving clinical states. These systems lack real-time adaptability, interpretability, and generalizability. With the increasing availability of high-frequency electronic medical record (EMR) data, machine learning (ML) approaches have emerged as powerful tools to model complex temporal patterns and support dynamic clinical decision-making. However, existing models are …

Methamphetamine Effects On The Striatal Neuroimmune System, Hillary Adelaide Wadsworth

Theses and Dissertations

Dopamine is thought of as the reward molecule of the brain, and while it does deal with reward, it is also associated with motivation and crucial for controlled movement. There are two main dopaminergic systems in the brain, the nigrostriatal system, which originates in the substantia nigra compacta and projects dopamine into the dorsal striatum; and the mesolimbic system, which originates in the ventral tegmental area and projects dopamine into the nucleus accumbens. While the mesolimbic dopamine system deals with motivation and reward, the nigrostriatal pathway underlies movement. Microglia are the immune cells of the brain and are incredibly dynamic. …

Maximizing Germination Of Three Native Plants For Rangeland Restoration And Urban Landscapes Through Seed Enhancements, Janetta Teichert

Theses and Dissertations

Native plants offer numerous ecological benefits and opportunities for both rangeland restoration and urban landscaping, making their integration essential for building sustainable ecosystems. They enhance soil health, promote pollinator activity, increase microbial diversity, and support both wildlife and livestock, all of which contribute to overall ecosystem resilience. A promising strategy to improve the use of native plants is the application of seed enhancement technologies, which can optimize seed germination, improve seedling establishment, and enhance overall plant success in challenging environments. These post-harvest seed treatments incorporate materials that improve seed performance, often through coatings composed of binders, fillers, and active ingredients. …

A Systematic Review And Meta-Analysis Of Chemical Sanitizer Efficacy Against Biofilms Of Listeria Monocytogenes, Salmonella Enterica, And Stec On Food Processing Surfaces, Allyson N. Hamilton, Sarah L. Jones, Christopher A. Baker, Xinya Liang, Adam Siepielski, Ashlynn Robinson, Gayatri R. Dhulappanavar, Kristen E. Gibson

Food Science Faculty Publications and Presentations

Chemical sanitizers are applied to food processing surfaces to inactivate bacterial pathogens. Pathogen type, surface type along with sanitizer type, concentration, and contact time are important factors potentially impacting sanitation efficacy. Numerous studies on chemical agents and lab-generated biofilms have been published; however, cross-study comparisons can be difficult. A systematic literature review (SLR) and meta-analysis were conducted to evaluate chemical sanitizer efficacy against Listeria monocytogenes, Salmonella spp., and Shiga toxin-producing Escherichia coli (STEC) within lab-generated biofilms on food contact surfaces (FCSs). The SLR included 13 peer-reviewed articles published between 2000 and 2020. Sanitizer concentration, type, contact time, surface type, and …

Vaccination Schedules Recommended By The Centers For Disease Control And Prevention: From Human-Readable To Machine-Processable, Aneesa Weaver, Chloe Crozier, David Robinson, Dean F Sittig, Paul G Biondich, Samuil Orlioglu, Akash Shanmugan Boobalan, Kojo Abanyie, Richard D Boyce, Adam Wright, Christian Nøhr, Timothy D Law, Arild Faxvaag, Lior Rennert, Ronald W Gimbel

Faculty, Staff and Student Publications

Background: Reusable, machine-processable clinical decision support system (CDSS) rules have not been widely achieved in the medical informatics field. This study introduces the process, results, challenges faced, and lessons learned while converting the United States of America Centers for Disease Control and Prevention (CDC)-recommended immunization schedules (2022) to machine-processable CDSS rules.

Methods: We converted the vaccination schedules into tabular, charts, MS Excel, and clinical quality language (CQL) formats. The CQL format can be automatically converted to a machine-processable format using existing tools. Therefore, it was regarded as a machine-processable format. The results were reviewed, verified, and tested.

Results: We have …

Swi/Snf Complex-Mediated Znf410 Cooperative Binding Maintains Chromatin Accessibility And Enhancer Activity, Siyuan Xu, Chuxuan Peng, Ren Ren, Haowen Lu, Han Zhao, Yijie Shen, Haoyue Zhang, Xiaodong Cheng, Gerd A Blobel, Xianjiang Lan

Faculty, Staff and Student Publications

The clustering of multiple transcription factor binding sites (TFBSs) for the same TF has proved to be a pervasive feature of cis-regulatory elements in the eukaryotic genome. However, the contribution of binding sites within the homotypic clusters of TFBSs (HCTs) to TF binding and target gene expression remains to be understood. Here, we characterize the CHD4 enhancers that harbor unique functional ZNF410 HCTs genome wide. We uncover that ZNF410 controls chromatin accessibility and activity of the CHD4 enhancer regions. We demonstrate that ZNF410 binds to the HCTs in a collaborative fashion, further conferring transcriptional activation. In particular, three ZNF410 motifs …

Can The Discovery Of High-Impact Diagnostics Be Improved By Matching The Sampling Rate Of Clinical Diagnostics To The Frequency Domain Of Diagnostic Information?, Steven W Millward, Peng Wei, David Piwnica-Worms, Seth T Gammon

Faculty, Staff and Student Publications

Over the past 30 years, academic and industrial research investigators have developed molecular reporters to visualize cell death in complex biological systems. In parallel, clinical researchers, chemists, biochemists, and molecular biologists have endeavored to translate these molecular tools into clinical imaging agents. Despite these efforts, there are no clinically approved imaging methodologies with which to image cell death consistently and quantitatively. One reason may reside in the intrinsic mismatch between the sampling frequency of translational molecular imaging and the biochemical kinetics that define cell death. Beyond cell death imaging, many active research programs are now attempting to create translational diagnostic …

Ensemble Learning With Explainable Ai For Improved Heart Disease Prediction Based On Multiple Datasets

Faculty, Staff and Student Publications

Heart disease is one of the leading causes of death worldwide. Predicting and detecting heart disease early is crucial, as it allows medical professionals to take appropriate and necessary actions at earlier stages. Healthcare professionals can diagnose cardiac conditions more accurately by applying machine learning technology. This study aimed to enhance heart disease prediction using stacking and voting ensemble methods. Fifteen base models were trained on two different heart disease datasets. After evaluating various combinations, six base models were pipelined to develop ensemble models employing a meta-model (stacking) and a majority vote (voting). The performance of the stacking and voting …

Genetic Regulation Of Anthocyanin Biosynthesis In Flower Color In Gilia, Veronica Mosquera

Theses and Dissertations

Gilia was the model species in this research to study the genetic regulation of flower color. I generated an F2 population from a cross of two closely related leafy-stemmed Gilia: G. capitata with blue and G. yorkii with white flowers, to obtain the fertile F1, which was selfed. The F2 population showed a segregation ratio between pigmented versus white flowers 3:1, suggesting a single locus with a major effect on the regulation of flower color. Then, the G. yorkii genome was assembled and annotated to become the reference genome to genotype-by-sequencing (GBS) 189 F2 individuals. The Quantitative Trait Loci (QTL) …

Lack Of Patient-Centered Care Relative To Evidence-Based Practice In Clinical Diagnosis And Treatment Of Endometriosis In The U.S.: A Review, Piper E. Scoskie

Honors Theses

This scoping review examines the impact of combining Patient-Centered Care (PCC) and Evidence-Based Care (EBC) on clinical outcomes and patient satisfaction in the diagnosis and treatment of endometriosis in the United States. In particular, the review aims to (1) examine the body of research from the United States to learn more about the connection between PCC and EBC and their collective impact on patient satisfaction and clinical outcomes in the treatment of endometriosis; (2) identify the gaps in existing research and practice, specifically regarding PCC procedures, varying patient populations, and longitudinal investigations; and (3) provide recommendation for future research and …

Silage Yield And Quality Of Different Grass Pea (Lathyrus Sativus L.) Genotypes, Murat Azak, Erdem Gülümser

Turkish Journal of Agriculture and Forestry

This study aimed to determine the silage yield and quality of different 12 grass pea (Lathyrus sativus L.) genotypes (Gap Mavisi, İptaş, Karadağ, 1603, 2006, 2401, 4301, 4403, 5001, 6408, 6410, and S3). The trial was established in Bilecik’s ecological conditions in 2022 and 2023. It was carried out according to the randomized block design with four replications, and the plants were harvested at the full flowering stage. The harvested plants were chopped to 2 cm in size and ensiled in vacuum bags and left to ferment at 25 ± 2 °C for 45 days. The silage yield, pH, dry …

Effect Of Different Mulch Materials Applications On Weed Control, Plant Characteristics, And Yield In Garlic (Allium Sativum L.), Ayşe Usanmaz Bozhüyük

Turkish Journal of Agriculture and Forestry

This study was conducted in plots under controlled conditions in the Silvan district of Diyarbakır Province in 2023. The local garlic genotype (Allium sativum L.) from Diyarbakır was used as the plant material. Three different organic mulching materials (sawdust, pine, and straw) were applied at a thickness of 5 cm. The effects of these mulching materials on leaf number, root length, plant height, number of cloves per plant, plant density, bulb diameter, dry and fresh weight of garlic, dry and fresh weight of weeds, weed density, and yield were analysed. A total of 34 weed species from 15 families were …