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Full-Text Articles in Entire DC Network
Modulation Of Cellular Energetics By Galactose And Pioglitazone., David Grimm, Leonardo Altamirano, Sudip Paudel, Leah Welker, Mary E. Konkle, Nilay Chakraborty, Michael Menze
Modulation Of Cellular Energetics By Galactose And Pioglitazone., David Grimm, Leonardo Altamirano, Sudip Paudel, Leah Welker, Mary E. Konkle, Nilay Chakraborty, Michael Menze
Faculty and Staff Scholarship
The Warburg effect is ameliorated by culturing transformed cells in the presence of galactose instead of glucose as the primary carbon source. However, metabolic consequences that are in addition to sensitizing the cells to mitochondrial toxins may occur. As such, the screening of pharmaceutical agents against transformed cells while using galactose must be carefully evaluated. Pioglitazone is used in clinical applications to treat type-2 diabetes, but clearly has other off target effects. Human hepatocellular carcinoma cells (HepG2) were cultured in glucose or galactosecontaining medium to investigate the role of pioglitazone on cellular bioenergetics employing calorimetry and respirometry. Compared to cells …
Chlamydomonas Reinhardtii, A Model For Quorum Sensing At The Interkingdom Interface, Timothy Charles Haire
Chlamydomonas Reinhardtii, A Model For Quorum Sensing At The Interkingdom Interface, Timothy Charles Haire
Theses and Dissertations
Microbial communities significantly impact the processes of the eukaryotic hosts they colonize. In many cases, symbiotic organisms both pathogenic and mutualistic coordinate their efforts through a process called quorum sensing (QS). QS is driven by low-molecular weight signals that regulate gene expression at threshold inducer concentrations, allowing phenotypic switching to be driven by cell density. These quorum sensing signals are subject to eukaryotic perception, and quorum associated phenotypes can directly influence the growth and development of eukaryotes. Eukaryotic perception of quorum sensing signals has been chiefly studied in plants, and to a lesser extent mammalian systems, yet no delineated model …
Modulation Of Cellular Energetics By Galactose And Pioglitazone., David Grimm, Leonardo Altamirano, Sudip Paudel, Leah Welker, Mary E. Konkle, Nilay Chakraborty, Michael A. Menze
Modulation Of Cellular Energetics By Galactose And Pioglitazone., David Grimm, Leonardo Altamirano, Sudip Paudel, Leah Welker, Mary E. Konkle, Nilay Chakraborty, Michael A. Menze
Faculty and Staff Scholarship
The Warburg effect is ameliorated by culturing transformed cells in the presence of galactose instead of glucose as the primary carbon source. However, metabolic consequences that are in addition to sensitizing the cells to mitochondrial toxins may occur. As such, the screening of pharmaceutical agents against transformed cells while using galactose must be carefully evaluated. Pioglitazone is used in clinical applications to treat type-2 diabetes, but clearly has other off target effects. Human hepatocellular carcinoma cells (HepG2) were cultured in glucose or galactosecontaining medium to investigate the role of pioglitazone on cellular bioenergetics employing calorimetry and respirometry. Compared to cells …
Advances In Developing Therapies To Combat Zika Virus: Current Knowledge And Future Perspectives, Ashok Munjal, Rekha Khandia, Kuldeep Dharma, Swati Sachan, Kumaragurubaran Karthik, Ruchi Tiwari, Yashpal S. Malik, Deepak Kumar, Raj K. Singh, Hafiz M. N. Iqbal, Sunil K. Joshi
Advances In Developing Therapies To Combat Zika Virus: Current Knowledge And Future Perspectives, Ashok Munjal, Rekha Khandia, Kuldeep Dharma, Swati Sachan, Kumaragurubaran Karthik, Ruchi Tiwari, Yashpal S. Malik, Deepak Kumar, Raj K. Singh, Hafiz M. N. Iqbal, Sunil K. Joshi
Bioelectrics Publications
Zika virus (ZIKV) remained largely quiescent for nearly six decades after its first appearance in 1947. ZIKV reappeared after 2007, resulting in a declaration of an international “public health emergency” in 2016 by the World Health Organization (WHO). Until this time, ZIKV was considered to induce only mild illness, but it has now been established as the cause of severe clinical manifestations, including fetal anomalies, neurological problems, and autoimmune disorders. Infection during pregnancy can cause congenital brain abnormalities, including microcephaly and neurological degeneration, and in other cases, Guillain-Barré syndrome, making infections with ZIKV a substantial public health concern. Genomic and …
The Effect Of Cocoa Flavanols On Β-Cell Mass And Function, Thomas John Rowley
The Effect Of Cocoa Flavanols On Β-Cell Mass And Function, Thomas John Rowley
Theses and Dissertations
A hallmark of type 2 diabetes (T2D) is β-cell dysfunction and the eventual loss of functional β-cell mass. Therefore, mechanisms that improve or preserve β-cell function could be used to improve the quality of life of individuals with T2D. Studies have shown that monomeric, oligomeric and polymeric cocoa flavanols have different effects on obesity, insulin resistance and glucose tolerance. We hypothesized that these cocoa flavanols may have beneficial effects on β-cell function. INS-1 832/13 derived β-cells and primary rat islets cultured with a monomeric catechin-rich cocoa flavanol fraction demonstrated enhanced glucose-stimulated insulin secretion, while cells cultured with total cocoa extract, …
Using Silkworms As A Host To Spin Spider Silk-Like Fibers, Xiaoli Zhang
Using Silkworms As A Host To Spin Spider Silk-Like Fibers, Xiaoli Zhang
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Using silkworms as the potential host to spin spider silk-like fibers is an area of intense research world-wide. The conventional methods used to create transgenic silkworms hosting spider silk-like gene limits the incorporation of spider silk-like protein and do not improve the mechanical performance of the composite silkworm/spider silk fibers. In this dissertation, synthetic spider ampullate genes were incorporated into the precise site of the fibroin heavy chain or light chain using the latest genome editing technology CRISPR/cas9 guided non-homologous end joining as opposed to conventional random integration using transposon-based piggyBac system. These protocols, with extensive applicability to other silkworm …
Functionalization Of Indium-Based Quantum Dots For Use As A Non-Viral Gene Therapy Vector, Nicholas A. Mundt
Functionalization Of Indium-Based Quantum Dots For Use As A Non-Viral Gene Therapy Vector, Nicholas A. Mundt
Graduate Theses/Dissertations
This work aims to develop functionalized, water-soluble indium-based quantum dots (QDs) as a non-viral gene therapy vector. The QDs were solubilized in water by exchanging native hydrophobic surface ligands with 11-mercaptoundecanioc acid (MUA); an amphiphilic ligand providing terminal carboxylate groups that impart water solubility to the QDs. The aqueous QDs were then functionalized with a terminal tertiary amine to impart a positive surface charge, allowing negatively-charged DNA to complex with the nanoparticles. The QDs were characterized via electrophoresis to determine their ability to bind DNA. Results show that further work is needed to optimize DNA binding. In addition, this work …
Microbial Community Richness Distinguishes Shark Species Microbiomes In South Florida, Rachael Cassandra Karns
Microbial Community Richness Distinguishes Shark Species Microbiomes In South Florida, Rachael Cassandra Karns
HCNSO Student Theses and Dissertations
The microbiome (microbial community) of individuals is crucial when characterizing and understanding processes that are required for organism function and survival. Microbial organisms, which make up an individual’s microbiome, can be linked to disease or function of the host organism. In humans, individuals differ substantially in their microbiome compositions in various areas of the body. The cause of much of the composition diversity is yet unexplained, however, it is speculated that habitat, diet, and early exposure to microbes could be altering the microbiomes of individuals (Human Microbiome Project Consortium, 2012b, 2012a). To date, only one study has reported on microbiome …
Lingnan Gardeners Newsletter (No. 32) = 嶺南彩園通訊 (第32期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter (No. 32) = 嶺南彩園通訊 (第32期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter 嶺南彩園通訊
Basil 羅勒花
The Ecology Of Acequias In The Mora Valley: Patterns, Processes And Place-Based Knowledge, Shannon Marie Rupert
The Ecology Of Acequias In The Mora Valley: Patterns, Processes And Place-Based Knowledge, Shannon Marie Rupert
Biology ETDs
In northern New Mexico, early settlements were clustered for protection of the people and access to water for domestic and agricultural uses was critical to their success. Irrigation ditches, known as acequias, brought water to the people, and were central to community life. These earthen ditches of varying lengths were built between one hundred and several hundred years ago, and most are still operational today. They divert water away from natural streams and across the landscape, through a system of man-made channels, until the unused water is diverted back into natural watercourses. These lateral channels appear to function as extensions …
Affordances In The Home Environment For Motor Development-Infant Scale, Spanish Translation, Danae Dinkel, Kailey Snyder, Priscila Cacola
Affordances In The Home Environment For Motor Development-Infant Scale, Spanish Translation, Danae Dinkel, Kailey Snyder, Priscila Cacola
Health and Kinesiology Faculty Publications
The home environment has a critical influence on an infant’s development and well-being. The Affordances in the Home Environment for Motor Development-Infant Scale (AHEMD-IS) is an instrument that has been developed to assess the home environment. This article illustrates the translation, validation, and cultural adaptation process of the AHEMD-IS from English to Spanish. The AHEMD-IS underwent a comprehensive process involving a four-phase translation process: (1) Forward translation, (2) Semantic equivalence, (3) Content equivalence testing, and (4) Final version development. Steps 1 and 2 resulted in linguistic alterations from the initial translation to enhance clarity for general public understanding. In step …
What Motivates Young Adults To Talk About Physical Activity On Social Network Sites?, Ni Zhang, Shelly Campo, Jingzhen Yang, Petya Eckler, Linda Snetselaar, Kathleen Janz, Emily Leary
What Motivates Young Adults To Talk About Physical Activity On Social Network Sites?, Ni Zhang, Shelly Campo, Jingzhen Yang, Petya Eckler, Linda Snetselaar, Kathleen Janz, Emily Leary
Faculty Research, Scholarly, and Creative Activity
Background:
Electronic word-of-mouth on social network sites has been used successfully in marketing. In social marketing, electronic word-of-mouth about products as health behaviors has the potential to be more effective and reach more young adults than health education through traditional mass media. However, little is known about what motivates people to actively initiate electronic word-of-mouth about health behaviors on their personal pages or profiles on social network sites, thus potentially reaching all their contacts on those sites.
Objective:
This study filled the gap by applying a marketing theoretical model to explore the factors associated with electronic word-of-mouth on social network …
The Power Of Prayer, Victoria Dawn Thompson
The Power Of Prayer, Victoria Dawn Thompson
Capstone Collection
If words are arbitrary, how does prayer have power?” is the question of inquiry in this paper. An unobtrusive Content Analysis inquiry methodology was used to answer this question. The answer lies in the finding that words and thoughts are not the same thing, and our thoughts expand beyond the audible and visible. The implication for professional practice these findings present is that a deeper awareness of “Self” is needed to understand people’s miraculous way of resolving conflict via prayer.
The Rpl13a Snorna U33 Forms Novel Snornps During Lipotoxicity, Miquia Sherree Henderson
The Rpl13a Snorna U33 Forms Novel Snornps During Lipotoxicity, Miquia Sherree Henderson
Arts & Sciences Graduate Student Theses and Dissertations
Lipid overload contributes to the pathogenesis of diabetic complications, causing tissue damage and cell death in a number of organ systems. This process is termed lipotoxicity. Animal and cell culture studies have demonstrated that oxidative stress and endoplasmic reticulum stress are major pathways engaged in the lipotoxic response. However, the molecular mechanisms of lipotoxicity are not well understood. A genetic screen revealed that small nucleolar RNAs (snoRNAs) encoded in the introns of the Rpl13a locus are critical for cell death in response to lipotoxicity. Initial studies have suggested that the Rpl13a snoRNAs function in this pathway through non-canonical modes of …
Precipitation Patterns And Fungal Community Succession In A Seasonally Dry Secondary Tropical Savanna, Sara R. López-Rodríguez
Precipitation Patterns And Fungal Community Succession In A Seasonally Dry Secondary Tropical Savanna, Sara R. López-Rodríguez
Biology ETDs
In the northwestern part of South America, seasonally dry forests have undergone extensive conversion to savannas to support ranching and agriculture. The combination of fire and vegetation removal employed for clearing, together with extensive agricultural practices, often lead to loss of soil fertility. This results in land that is no longer useful for agriculture and on which historical forests are unable to regenerate. This study examined the effects of common agricultural practices on soil fungal communities by simulating the effects of fire followed by plant removal. The goal was to determine whether common practices have rapid effects on soil communities, …
Bio Buzz Newsletter, Issue 13, May 2017, Department Of Biology
Bio Buzz Newsletter, Issue 13, May 2017, Department Of Biology
Biology Buzz Newsletter
Denison University, Department of Biology newsletter for Spring 2017.
Native Science In Practice: Cases For Broadening Understanding And Engagement Of Science In Education As A Plea For Future Generations, G. Sue Kasun, Dave López
Native Science In Practice: Cases For Broadening Understanding And Engagement Of Science In Education As A Plea For Future Generations, G. Sue Kasun, Dave López
Journal of Multicultural Affairs
We make a departure from the U.S.’s traditional “science education” in recognition of several stark realities—from the genocidal practices that have eliminated so many indigenous populations in the U.S. to the problems our planet faces, such dire environmental degradation. We are faced daily with a dissonance of knowing our planet needs our respect and care while at the same time bearing witness to “modern” lifestyles based in property rights and individualism. We engage Native science—which encapsulates all of Western science and expands upon it by including the spiritual and emotional realms as well as the physical and mental dimensions of …
Mapping The Interaction Between Lrrc59 And Cip2a Oncoprotein, Tamika C. Reed
Mapping The Interaction Between Lrrc59 And Cip2a Oncoprotein, Tamika C. Reed
Graduate School of Biomedical Sciences Theses and Dissertations
The oncogene cancerous inhibitor of protein phosphatase 2A (CIP2A) has been shown to promote oncogenesis through numerous protein-protein interactions. CIP2A was initially found to be a direct inhibitor of the PP2A tumor suppressor protein; however, new research has demonstrated that CIP2A can act independently of PP2A through protein-protein interactions resulting in deregulation of the cell cycle and the development of therapeutic drug resistance, tumorigenesis, and cell proliferation. It has been shown that leucine rich repeat containing 59 protein (LRRC59) binds to and is required for the nuclear translocation of CIP2A, thereby making this interaction a target for drug therapy. Thus, …
Aquaponics: Redefining Education For Our Youth, Joshua Peal
Aquaponics: Redefining Education For Our Youth, Joshua Peal
Senior Theses
In America, we are seeing a lack of effectiveness with our K-12 schools. This costs the school districts and taxpayers money with very little return guaranteeing an ongoing investment into adulthood. Students finishing high school have very little if any applied learning and technical skills, and still need to accomplish at least four years of college or a trade school to be able to compete, even for a job not requiring a degree. Of those high school graduates, half and growing do not feel that they are prepared for college. Students also have little understanding of, or experience with, the …
An Evaluation Of Gabab Receptors On Modulating Neuroinflammation In A Non-Transgenic Animal Model Of Alzheimer's Disease, Monica Bolton
An Evaluation Of Gabab Receptors On Modulating Neuroinflammation In A Non-Transgenic Animal Model Of Alzheimer's Disease, Monica Bolton
UNLV Theses, Dissertations, Professional Papers, and Capstones
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive memory loss and distinct neuropathological hallmarks, including amyloid beta plaques and neurofibrillary tau tangles (NFT). Although the etiology remains to be discovered, several risk factors exist that significantly contribute to developing AD. Diabetes is one of the major risk factors associated with AD and is characterized by disrupted insulin signaling that may contribute to or exacerbate AD pathologies. Furthermore, both disorders result in increased neuroinflammation. Considerable evidence has demonstrated that a chronic inflammatory response, in particular chronic microglia activation, promotes A production as well as the hyperphosphorylation of tau through …
Observations Of Xyleborus Affinis Eichhoff (Coleoptera:Curculionidae:Scolytinae) In Central Michigan, Richard A. Roeper, Mark A. Bunce, John E. Harlan, Richard G. Bowker
Observations Of Xyleborus Affinis Eichhoff (Coleoptera:Curculionidae:Scolytinae) In Central Michigan, Richard A. Roeper, Mark A. Bunce, John E. Harlan, Richard G. Bowker
The Great Lakes Entomologist
Xyleborus affinis Eichhoff colonized wind thrown timber in the moist floodplain habitats of Central Michigan. Single adult females constructed a complex gallery system consisting of phloem–sapwood interface tunnels and sapwood tunnels. An average of 24 progeny adults and a sex ratio of 14 females to 1 male were found in mature galleries after the first of September.
Playing-With The World: Toy Story's Aesthetics And Metaphysics Of Play, Jonathan Hendricks
Playing-With The World: Toy Story's Aesthetics And Metaphysics Of Play, Jonathan Hendricks
USF Tampa Graduate Theses and Dissertations
Pixar’s Toy Story (John Lassiter, 1995) is not just a story about toys and the children that play with them, but a demonstration of how we interact with the world. This thesis looks at the way in which both main children, Andy and Sid, interact with their toys and how this interaction is one that is structured by way of what Martin Heidegger calls “Enframing.” In this modality of playing, toys and other things and entities in the world, and the world itself, appear to the children as on-hand resources for use at any time and can be molded, as …
Coccolithoviruses: A Review Of Cross-Kingdom Genomic Thievery And Metabolic Thuggery, Jozef I. Nissimov, António Pagarete, Fangrui Ma, Sean Cody, David D. Dunigan, Susan A. Kimmance, Michael J. Allen
Coccolithoviruses: A Review Of Cross-Kingdom Genomic Thievery And Metabolic Thuggery, Jozef I. Nissimov, António Pagarete, Fangrui Ma, Sean Cody, David D. Dunigan, Susan A. Kimmance, Michael J. Allen
Nebraska Center for Virology: Faculty Publications
Coccolithoviruses (Phycodnaviridae) infect and lyse the most ubiquitous and successful coccolithophorid in modern oceans, Emiliania huxleyi. So far, the genomes of 13 of these giant lytic viruses (i.e., Emiliania huxleyi viruses—EhVs) have been sequenced, assembled, and annotated. Here, we performed an in-depth comparison of their genomes to try and contextualize the ecological and evolutionary traits of these viruses. The genomes of these EhVs have from 444 to 548 coding sequences (CDSs). Presence/absence analysis of CDSs identified putative genes with particular ecological significance, namely sialidase, phosphate permease, and sphingolipid biosynthesis. The viruses clustered into distinct clades, based on their DNA polymerase …
Lingnan Gardeners Newsletter (No. 28) = 嶺南彩園通訊 (第28期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter (No. 28) = 嶺南彩園通訊 (第28期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter 嶺南彩園通訊
Chive Flowers 韭菜花
Niche By Basma Kavanagh, Vivian M. Hansen
Lingnan Gardeners Newsletter (No. 27) = 嶺南彩園通訊 (第27期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter (No. 27) = 嶺南彩園通訊 (第27期), Lingnan Gardeners, Kwan Fong Cultural Research And Development Programme, Lingnan University
Lingnan Gardeners Newsletter 嶺南彩園通訊
Lunar New Year Day 元日
Genomic Characterization Of Dairy Associated Leuconostoc Species And Diversity Of Leuconostocs In Undefined Mixed Mesophilic Starter Cultures, Cyril A. Frantzen, Witold Kot, Thomas B. Pederson, Ylva M. Ardo, Jeff Broadbent, Horst Neve, Lars H. Hansen, Fabio Dal Bello, Hilde M. Østlie, Hans P. Kleppen, Finn K. Vogensen, Helge Holo
Genomic Characterization Of Dairy Associated Leuconostoc Species And Diversity Of Leuconostocs In Undefined Mixed Mesophilic Starter Cultures, Cyril A. Frantzen, Witold Kot, Thomas B. Pederson, Ylva M. Ardo, Jeff Broadbent, Horst Neve, Lars H. Hansen, Fabio Dal Bello, Hilde M. Østlie, Hans P. Kleppen, Finn K. Vogensen, Helge Holo
Nutrition, Dietetics, and Food Sciences Faculty Publications
Undefined mesophilic mixed (DL-type) starter cultures are composed of predominantly Lactococcus lactis subspecies and 1–10% Leuconostoc spp. The composition of the Leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. The scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. We present data on the Leuconostoc population in five DL-type starter cultures commonly used by the dairy industry. The analyses were performed using traditional cultivation methods, and further augmented by next-generation DNA sequencing …
The Slow Food Movement: A 'Big Tent' Ideology, Mark N. Wexler, Judy Oberlander, Arjun Shankar
The Slow Food Movement: A 'Big Tent' Ideology, Mark N. Wexler, Judy Oberlander, Arjun Shankar
Journal of Ideology
The Slow Food Movement (SFM) has been characterized as a reminder of the centrality of the kitchen in the “good old days”. It has also been referred to as a poor use of science posing as a rallying point for the beleaguered middle class trying to reclaim psychological territory lost to the fast-paced commercialization of what was once private or leisure time. This paper argues that both these criticisms contain more than a kernel of truth. However, each misses the mark when it comes to explaining the relative successes of SFM. In this paper, we explore SFM’s basic ideological premises. …
Tsu Faculty Research Database-Jan 2017, David Owerbach
Tsu Faculty Research Database-Jan 2017, David Owerbach
Office of Research Institutional Research and Scholarship
Research interests and selected publications from 230 Texas Southern University faculty have been updated in Jan 2017. Faculty from Public Affairs, the College of Science, Engineering and Technology, the College of Pharmacy and Health Sciences, the College of Education, the College of Business, the College of Liberal Arts and Behavioral Sciences, the Law School and the School of Communications are included.
Myogenic Progenitor Cells Control Extracellular Matrix Production By Fibroblasts During Skeletal Muscle Hypertrophy, Christopher S. Fry, Tyler J. Kirby, Kate Kosmac, John J. Mccarthy, Charlotte A. Peterson
Myogenic Progenitor Cells Control Extracellular Matrix Production By Fibroblasts During Skeletal Muscle Hypertrophy, Christopher S. Fry, Tyler J. Kirby, Kate Kosmac, John J. Mccarthy, Charlotte A. Peterson
Physiology Faculty Publications
Satellite cells, the predominant stem cell population in adult skeletal muscle, are activated in response to hypertrophic stimuli and give rise to myogenic progenitor cells (MPCs) within the extracellular matrix (ECM) that surrounds myofibers. This ECM is composed largely of collagens secreted by interstitial fibrogenic cells, which influence satellite cell activity and muscle repair during hypertrophy and aging. Here we show that MPCs interact with interstitial fibrogenic cells to ensure proper ECM deposition and optimal muscle remodeling in response to hypertrophic stimuli. MPC-dependent ECM remodeling during the first week of a growth stimulus is sufficient to ensure long-term myofiber hypertrophy. …