Digital Commons Network^™

An Evaluation Of The Nebraska Ffa Launch! Program, Miranda M. Hornung

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

The purpose of this program evaluation was to discover the experiences of entrepreneurial education received in the Nebraska FFA Launch! Program in relation to future decisions for high school students about entrepreneurship. The Launch! Program aims to provide an educational experience for youth to develop an entrepreneurial mindset and view entrepreneurship as a possible career pathway. This focus addresses the opportunity to potentially help solve the issue of brain drain in rural Nebraska communities. A program evaluation was conducted by story harvesting from past participants through Zoom interviews to understand personal experiences and takeaways from their time in the Launch! …

Subjective Feelings And Electrophysiological Responses To Love-Stimuli In Parasocial And Real-Life Romantic Love, Magda Martinez-Mata

Theses

People can have a crush on, fall in love with, or develop a romantic interest in media figures despite having no reciprocal interaction, which is known as parasocial love. The objective of this study was to evaluate subjective feelings for and event-related potential (ERP) responses reflecting attention to the parasocial beloved and to compare them to real-life love. Twenty-six participants who have a parasocial beloved were recruited. Sixteen of those had a real-life beloved as well. The participants answered questions about their love feelings and completed the Infatuation and Attachment Scales and the Parasocial Love Scale for both their parasocial …

Effect Of Different Pellet Binders On Palm Kernel On Growth Performance And Nutrient Digestibility In Broiler Chicken, Nuun Marfuah, Burhanudin Sundu, Rusdi Rusdi, Sugiarto Sugiarto

Jurnal Ilmu Ternak Universitas Padjadjaran

Feed plays a paramount role in supporting growth, metabolism, and production performance. Their high price encourages the utilization of inexpensive and alternative feed ingredients. PKM, one such feed ingredient, is produced by palm oil industries. However, there is still a very limited application because of its high crude fiber content, which needs processing technologies like pelletization with binder addition. Based on this background, this experiment has been conducted to study the effect of various types of natural and commercial binders in 15% PKM-based pellets on performance and nutrient digestibility of broilers. The study was done for five weeks using 150 …

Evaluating The Impact Of K135t Mutation On Malate Dehydrogenase Kinetic Activity, Emily Frost, Kaitlyn Mello

Undergraduate Research Symposium (2026- )

Malate Dehydrogenase (MDH) is a key protein in the citric acid cycle; its dysregulation can have catastrophic impacts, such as neurological and metabolic disorders, and various cancers. MDH catalyzes the reversible oxidation-reduction reaction between malate and oxaloacetate, also reducing NAD+ to NADH in the process. The citric acid cycle is one of the central metabolic pathways, connecting to other essential pathways, such as amino acid and fatty acid biosynthesis, highlighting the importance of understanding how MDH works. A key site in MDH is the flexible loop region (amino acids 119-137), responsible for substrate binding, catalysis, and orientation. The flexibility of …

Biophysical Insights Into A Cryptic, Hydrophobic Binding Site On Pcna, Alexis Dispensa

Theses and Dissertations

Proteins are dynamic molecules whose functional landscapes extend beyond static structural representations. Transient cryptic pockets (buried cavities that emerge through protein motion) represent underexplored targets for allosteric regulation and drug discovery. General anesthetics are small, lipophilic, weakly polar molecules that are well suited to partition into such hydrophobic sites. In this work, environment-sensitive fluorescence, photoaffinity labeling, mass spectrometry, mutational analysis, and microsecond molecular dynamics simulations were integrated to discover and characterize a cryptic, highly apolar binding site within the hydrophobic core of human proliferating cell nuclear antigen (PCNA), a central scaffold in DNA replication and repair. Using the solvatochromic fluorescent …

Evaluating Sand Characteristics And Their Influence On Sea Turtle Nest Incubation Conditions, Thomas Naylor

Honors Theses

The embryonic development of sea turtles is strongly influenced by the environmental factors experienced during incubation within the nest. These environmental factors can influence hatchling sex ratios, body condition, and their success emerging from nests. Adult female sea turtles lay nests across a range of sites throughout the nesting season, resulting in each nest encountering unique abiotic conditions. This study aims to identify how variation in sand characteristics, such as grain size proportions, permeability, and color, influences nest temperature and subsequent hatchling outcomes of Loggerhead Sea Turtles (Caretta caretta) and Green Sea Turtles (Chelonia mydas) from …

Exploring Undergraduate Student’S Perceptions Of Agriculture, Cayden J. Barton

Honors Theses

Americans today, both young and old, face issues with agricultural knowledge regarding the food and fiber industry and production agriculture as a whole (Frick et al., 1995). In a society where consumers are separated from production agriculture, public interaction with agriculture is less prevalent and the importance of agriculture is undervalued. A person’s knowledge of agriculture, or agricultural literacy affects the way that they perceive agricultural commodities and the agricultural industry (Specht, McKim, & Rutherford, 2014). Without adequate knowledge of the agricultural industry consumers cannot make well informed decisions about food safety or processing methods. In addition to consumers, government …

Brown Pelican (Pelecanus Occidentalis) Groundings On A Four-Lane Divided Highway In South Texas: A Comparative Study Using Citizen Science, Bradley Evan Beer, Kevin W. Ryer, Md. Saydur Rahman, John H. Young Jr., Richard J. Kline

School of Integrative Biological & Chemical Sciences Faculty Publications

The population of the once-endangered brown pelican (Pelecanus occidentalis) has increased over the past several decades and is now considered fully recovered. However, in south Texas, the combined effects of weather patterns, transportation infrastructure, and brown pelican biology have led to road mortalities on State Highway 48 (SH 48) at the Carl “Joe” Gayman Bridge. To mitigate these mortalities, the Texas Department of Transportation modified traffic barriers on SH 48. We aimed to examine the effect of the barrier change and determine which weather variables influence brown pelican groundings on SH 48. Citizen scientists monitored sections of SH …

Semiochemicals And Odorant Receptors Underlying Potato Cultivar Susceptibility And Resistance To Potato Tuber Moth, Ruipeng Chen, Wangtao Hu, Qin Hu, Junjie Yan, Jiao Yin, Fathiya M. Khamis, Yulin Gao, Walter S. Leal

All Peer-Reviewed Publications

Infestation of potato by the potato tuber moth (Phthorimaea operculella) varies markedly among cultivars, yet the chemical and molecular mechanisms underlying this variation remain poorly understood. Here, we combine field surveys, chemical ecology, functional genomics, and structural modeling to reveal how cultivar-specific volatile profiles shape moth oviposition behavior and pest outcomes. Field and laboratory assays identified marked differences in adult attraction, oviposition, and larval damage among potato cultivars, which correlated with distinct blends of emitted volatile organic compounds (VOCs). Behavioral analyses revealed that among other VOCs, trans-nerolidol and β-ionone act as attractants, whereas 3-carene and benzyl tiglate function as oviposition …

Three-Dimensional Imaging Of Neuronal Activation Patterns In Chronic Neuropathic Pain And Opioid Pharmacology, Madeline Martinez

Electronic Theses and Dissertations 2020 - Present

Chronic neuropathic pain severely diminishes quality of life, yet the supraspinal neural circuits that sustain pain perception and mediate analgesic relief remain incompletely characterized. While peripheral and spinal pain pathways are well established, relatively few studies have mapped pain- or opioid-related neuronal activation across the entire brain. This dissertation employed activity-dependent genetic labeling (TRAP2/Ai9), whole-brain tissue clearing, light sheet microscopy, and graph-theoretic network analysis to characterize brain-wide activation patterns across chronic pain and analgesic conditions in mice.

In Chapter 1, we mapped supraspinal neuronal activation in a bilateral spared nerve injury model of chronic neuropathic pain. Although no individual region …

Mechanistic Insights Into E. Coli Recovery From Growth Arrest, Ahmed H. Hassan, Yuko Nakano, Howard Gamper, Isao Masuda, Ludmila Vesela, Matyas Pinkas, Sathya Narayanan Nagarajan, Jonathan Dworkin, Gregor Blaha, Ya-Ming Hou, Gabriel Demo

Department of Biochemistry and Molecular Biology Faculty Papers

Bacteria survive hostile conditions by shutting down protein synthesis, but how they restart growth remains poorly understood. Here, we use an E. coli ΔrimM strain, which exhibits a prolonged growth arrest, as a model to investigate how bacteria recover from this state and restore protein synthesis. RimM is a conserved ribosome maturation factor for the 3'-major (head) domain of the 16S rRNA within the bacterial 30S subunit. The loss of RimM causes a longer delay in recovery than other 30S maturation factors, including RbfA. Cryo-EM analysis of ΔrimM ribosomes suggests a delayed recruitment of ribosomal proteins to the 30S head …

Testing Effects Of Acoustic Propagation, Morphology, And Intrasexual Selection On The Elaboration And Performance Of Song In Bachman’S Sparrows (Peucaea Aestivalis), Hans Kayser Gonzembach

Electronic Theses and Dissertations 2020 - Present

Birdsong is a communication signal shaped by ecological, morphological, and cultural processes. While many songbird species produce relatively simple vocal repertoires, others, such as the Bachman’s Sparrow (Peucaea aestivalis), produce moderately large repertoires with multiple distinct and context-dependent song categories. Despite this complexity, little is known about the major drivers that shape song elaboration in this species. Additionally, it is unclear whether abiotic factors play a significant role in determining how different song types are learned and shared within a population. To better understand how ecological and social pressures shape song variation, I conducted three studies using Bachman’s …

Living With Pets: The Science Behind Immune Development, Mitchell Wood

Osmosis Magazine

No abstract provided.

Seeing The Future: How Chemically Modified Vitamin A Slows Blindness, Marty Snyder

Osmosis Magazine

No abstract provided.

Milk-Fed Astronauts In A Microbial Galaxy, Charlie Lanning

Osmosis Magazine

No abstract provided.

Rna Interference Targeting Autocrine Motility Factor Receptor In Unfolded Protein Response In Acyrthosiphon Pisum, Micah A. Von Stroh, James Balthazor

SACAD: Scholarly Activities

Acyrthosiphon pisum, more commonly known as pea aphids, are a pest to many species of Fabaceae (legumes) mainly due to the species being prone to carrying Fabaceae diseases. Protection against A. pisum currently includes insecticides and natural predators, both of which bring potential negative effects to other organisms in the surrounding area. In this study, the use of RNA interference (RNAi) provides an alternative and species-specific elimination of A. pisum. The targeted proteins in this study, Autocrine Motility Factor Receptor, plays a role in removing misfolded proteins from the cells of A. Pisum and is encoded by the AMFR gene. …

Structural Transformation Of A Hydrogel-Forming Cell Division Protein Zapb Of Multidrug Resistant Klebsiella Pneumoniae With Small Molecules, Ayesha Khan, M. Iqbal Choudhary, Sumaira Javaid, Humaira Zafar, Innokentiy Maslennikov, Atia-Tul Wahab

Pharmacy Faculty Articles and Research

Septal ring assembly protein ZapB (EMR18431.1) is involved in Z-ring formation, and nucleoid segregation during the bacterial cell division . It promotes cell division in the initial stages of the cell cycle through direct interaction with FtsZ, thus stimulating Z-ring assembly. The ZapB inhibition can make bacterial cell susceptible to primary antibiotics, and therefore considered as an important approach for adjuvent therapy against MDR bacterial infections. Hence in the current study, ZapB protein of multidrug resistant Klebsiella pneumoniae was cloned, and expressed in E. coli system. To understand its role, and to assess the mechanism of ZapB activation, the characteristic …

Redox Reprogramming In Neurogenesis: Candidate Developmental Switches And Targets Of Oxidative Toxicity In The Redox Proteome, Ryan Jacob Summerhays

Theses and Dissertations

Neurogenic differentiation requires coordinated redox regulation, yet how reversible cysteine oxidation is remodeled at proteome scale during this process remains poorly defined. Here, we asked whether differentiation is associated with selective, site-specific changes in reversible cysteine oxidation and whether oxidative stress reveals developmentally conditioned vulnerabilities within this redox landscape. To address this, we applied a discovery-oriented, proteome-scale redox profiling strategy that quantifies condition-dependent changes in reversible cysteine oxidation using CysPAT labeling, phosphopeptide enrichment, and multiplexed TMT mass spectrometry. Neurogenic differentiation was characterized by reproducible, bidirectional, and highly site-specific remodeling of reversible cysteine oxidation at discrete regulatory nodes, as well as …

Singing Not To Sing: The Interplay Of The Environment And Behavior On The Reproductive Success Of Forest Breeding Migratory Birds, Miranda Bliss Zammarelli

Dartmouth College Ph.D Dissertations

Habitat selection is a key behavior that has consequences for fitness, which can shift with environmental variation. The environment can affect reproductive success indirectly by shaping behavior or via direct impacts. Many forest breeding migratory birds choose a habitat by establishing territories. To understand the interplay of environment and behavior on the reproductive success of forest breeding migratory birds, I leveraged long-term data and newly collected species-specific data from Hubbard Brook Experimental Forest, New Hampshire, USA. I analyzed over 50 years of territory maps from seven species occupying a 10-ha plot to understand how birds distribute themselves in a habitat. …