Digital Commons Network^™

Learn By Doing: A Flipped Classroom Approach To Teaching Exercise Testing And Prescription, Zakkoyya H. Lewis

Educational Practices in Kinesiology

The purpose of this paper is to describe a case-study based flipped classroom approach for teaching exercise testing and prescription. The goal of this approach is to provide students with supervised “real world” experience while also exposing students to diverse populations (e.g. health, age, culture, employment, ability). Students will complete a client interview, conduct a complete fitness assessment included a graded exercise test, and develop an individualized exercise prescription. The flipped classroom is intended to be incorporated into an in-person advanced level course, ideally less than 20 students per section. Completion of an introductory level exercise testing and prescription course …

Winter Fungi Of The North Woods, Abraham F. Stone, Lane Cozort, Isabelle Holmstrom, Tara Bal

Open Access Books

A simple field guide to assist in identifying winter fungi in northern hardwood forests. This guide is focused on woody species easily found persisting in winter above snow level and with few look-a-likes found in northeastern and north-central North America. Photos and descriptions are from the Upper Peninsula, Michigan.

The Bacterial Microbiome Modulates The Initiation Of Brain Metastasis By Impacting The Gut-To-Brain Axis, Matteo Massara, Michelle Ballabio, Bastien Dolfi, Golnaz Morad, Vladimir Wischnewski, Joao Lourenco, Hector Gallart-Ayala, Rui Santalla Méndez, Annamaria Kauzlaric, Nadine Fournier, Ashish V Damania, Matthew C Wong, Julijana Ivanisevic, Nadim J Ajami, Jennifer A Wargo, Johanna A Joyce

Faculty, Staff and Student Publications

Brain metastases (BrMs) are the most common brain tumors in patients and are associated with poor prognosis. Investigating the systemic and environmental factors regulating BrM biology represents an important strategy to develop effective treatments. Toward this goal, we explored the contribution of the gut microbiome to BrM development by using in vivo breast-BrM models under germ-free conditions or antibiotic treatment. This revealed a detrimental role of gut microbiota in fostering BrM initiation. We thus evaluated the impact of antibiotics and BrM outgrowth on the gut-brain axis. We found the bacterial genus Alistipes was differentially present under antibiotic treatment and BrM …

Immune Checkpoint Inhibitors Plus Debulking Surgery For Patients With Metastatic Renal Cell Carcinoma: Clinical Outcomes And Immunological Correlates Of A Prospective Pilot Trial, Sangeeta Goswami, Jianjun Gao, Sreyashi Basu, Daniel D Shapiro, Jose A Karam, Kamran Ahrar, Yu Shen, Alexandro E Trevino, Aaron T Mayer, Alexsandra B Espejo, Christian Seua, Marc D Macaluso, Yulong Chen, Wenbin Liu, Zhong He, Shalini S Yadav, Ying Wang, Priya Rao, Li Zhao, Jianhua Zhang, Sonali Jindal, Nizar M Tannir, Andrew Futreal, Linghua Wang, Padmanee Sharma

Faculty, Staff and Student Publications

Surgical removal of primary tumors reverses tumor-mediated immune suppression in pre-clinical models with metastatic disease. However, how cytoreductive surgery in the metastatic setting modulates the immune responses in patients, especially in the context of immune checkpoint therapy (ICT), is not understood. We report the first prospective, pilot, non-comparative clinical trial (NCT02210117) to evaluate the feasibility, clinical benefits, and immunologic changes of combining three different ICT-containing strategies with cytoreductive surgery or biopsy for patients with metastatic clear cell renal cell carcinoma. Primary safety endpoint of this trial has been met, with 43 patients completing cytoreductive surgery, 36 patients undergoing …

Agile Project Management And The Future Of R&D, Aditi Vinayak Nalavade

Harrisburg University Dissertations and Theses

The use of Agile methodologies in research and development (R&D) sectors is changing the project management landscape by profoundly altering approaches to innovation and execution. This research will delve into the benefits and challenges of implementing Agile in an R&D setting, with a particular focus on the increasing significance of AI. The research's central theme offers a comprehensive analysis of how Agile approaches are shaping the future of R&D. Agile's iterative process, with its high degree of adaptability, flexibility, and iterative methods, is particularly beneficial in R&D, where reshaping research teams' approaches to creativity and problem-solving even in the most …

Aif3 Splicing Variant Elicits Mitochondrial Malfunction Via The Concurrent Dysregulation Of Electron Transport Chain And Glutathione-Redox Homeostasis, Mi Zhou, Shuiqiao Liu, Yanan Wang, Bo Zhang, Ming Zhu, Veena Rajaram, Weibo Luo, Yingfei Wang

Faculty, Staff and Student Publications

Genetic mutations in apoptosis-inducing factor (AIF) have a strong association with mitochondrial disorders; however, little is known about the aberrant splicing variants in affected patients and how these variants contribute to mitochondrial dysfunction and brain development defects. We identified pathologic AIF3/AIF3-like splicing variants in postmortem brain tissues of pediatric individuals with mitochondrial disorders. Mutations in AIFM1 exon-2/3 increase splicing risks. AIF3-splicing disrupts mitochondrial complexes, membrane potential, and respiration, causing brain development defects. Mechanistically, AIF is a mammalian NAD(P)H dehydrogenase and possesses glutathione reductase activity controlling respiratory chain functions and glutathione regeneration. Conversely, AIF3, lacking these activities, disassembles mitochondrial complexes, increases …

Escape From Diabetes, J E. Jochum, J Carmack, J Toon

Clinical Practice in Athletic Training

Introduction: In the past decade, interprofessional education (IPE) to promote interprofessional collaborative practice has become an integral component of pre-licensure education in the health professions. Research shows that IPE activities involving problem-solving in patient care that requires cooperation and communication among the professions is most effective. An effective activity to meet interprofessional competencies is the use of escape rooms. Escape rooms are team-based games where groups of individuals solve puzzles to accomplish a specific goal, typically in a limited amount of time. There are many benefits to using escape rooms in education, including promoting problem-solving, teamwork, and creativity, improving focus …

Rural Entrepreneurship In Upper Egyptian Communities: Exploring The Intervention Of Agri-Tech Startups, Marwa Wael Abohamda Hulail

Theses and Dissertations

Climate change and economic disturbance have changed the state of the world and increased the dependence on digitization in running people's daily life. In Egypt, the economic instability and climate change have affected people’s standard of living, making it challenging to sustain their life, especially in the agriculture sector. Farmers have been affected by the risks of climate change, economic shocks, and trends of digitization, which have impacted their profit, employability, and livelihood.

Several Egyptian Agri-tech startups were launched to support the farmers in the digital revolution that came to agriculture, attempting to make a change in the Egyptian farming …

“Phytochemical Analysis, In Vitro Anti-Oxidant, Anti-Diabetic And Anti-Inflammatory Activity Of Red Kidney Bean (Phaseolus Vulgaris L.) ”, Nancy Mani, D Annette Beatrice, R Durga Priyadarshini

International Journal of Health and Allied Sciences

Aims: The aim of the study is to assess the phytochemicals, bioactive compounds and determine the in vitro anti-oxidant, anti-diabetic and anti-inflammatory potential of red kidney bean.

Methods and Material: Raw and cooked red kidney bean (Phaseolus vulgaris. L) were assessed for phytochemical content, anti-oxidant, anti-diabetic and anti-inflammatory activity and GC-MS analysis was done to identify the bioactive compounds.

Statistical analysis used: Descriptive statistics, independent ‘t’ test and one-way ANOVA was computed to determine the differences in the phytochemical compounds and in vitro activities of raw and cooked red kidney beans.

Results: Qualitative analysis of raw and …

Natural And Bioengineered Extracellular Vesicles In Diagnosis, Monitoring And Treatment Of Cancer, Xin Luo, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs) are cell derived nanovesicles which are implicated in both physiological and pathological intercellular communication, including the initiation, progression, and metastasis of cancer. The exchange of biomolecules between stromal cells and cancer cells via EVs can provide a window to monitor cancer development in real time for better diagnostic and interventional strategies. In addition, the process of secretion and internalization of EVs by stromal and cancer cells in the tumor microenvironment (TME) can be exploited for delivering therapeutics. EVs have the potential to provide a targeted, biocompatible, and efficient delivery platform for the treatment of cancer and other …

Profile Analysis Of Handwashing Behavior Among A Sample Of College Students In The Multi-Theory Model Framework, Miguel Antonio Fudolig, Robert E. Davis, Kavita Batra, Manoj Sharma

Health, Human Performance and Recreation Faculty Publications and Presentations

Maintaining proper hand hygiene is crucial in preventing the spread of infections and other communicable diseases. It is imperative to determine the factors that affect the likelihood of initiating and maintaining the recommended handwashing behavior, especially during a pandemic. This quantitative, secondary study employed a latent profile analysis (LPA) to identify the different attitudes toward behavior change based on the Multi-Theory Model (MTM) framework in the context of following the guidelines provided by the Centers for Disease Control and Prevention (CDC) during the COVID-19 pandemic. Data were collected from 602 college students at a large university in the southern region …

Mitochondrial Dysfunction Is A Hallmark Of Woody Breast Myopathy In Broiler Chickens, Elizabeth S. Greene, Paula R. Chen, Carrie Walk, Mike Bedford, Sami Dridi

Poultry Science Faculty Publications and Presentations

The woody breast (WB) myopathy poses significant economic and welfare concerns to the poultry industry, however, there is no effective strategy to mitigate this pathology due to its unknown etiology. After showing previously that hypoxia is a key factor in WB progression, we used here various techniques demonstrating dysregulated mitochondria (morphology, biogenesis, tethering, function, and bioenergetics) in WB-affected muscles and in hypoxic myoblasts compared to healthy tissues and normoxic cells, respectively. The increased levels of calcium (Ca2+) in both WB-affected tissues and hypoxic myoblasts suggested that mitochondrial Ca2+ overload is likely a leading cause for mitochondrial dysfunction that merits further …

Lisa Rogers, Lisa Rogers, Institute Of Child Nutrition

Oral History Project (all interviews)

Lisa Rogers is a native of of Tennessee. Lisa’s professional journey includes roles in advertising, library services, and recruitment prior to getting into child nutrition. Lisa joined the Institute of Child Nutrition as a Library Specialist, eventually becoming the Associate Director of the Training Division. Lisa retired on June 30, 2023.

The New American Farmer – Extension Engagement With Urban Agriculture And Food Systems, Margaux S. C. Robinson, Kc V. Cunningham, Jeffery A. Young, Nicole D. Breazeale, Kenneth R. Jones, Julie N. Zimmerman

Journal of Extension

Extension’s evolving role in urban food production will require intensive reflexivity and ongoing collaboration. Extension educators around the country have already made progress in engaging with both the social and horticultural sides of urban agriculture. Designed appropriately, urban food systems hold the potential for healthy food access, community and environmental resilience, and economic prosperity (Rangarajan & Riordan 2019). Moving forward, we offer recommendations for Extension staff to apply within their institutions and beyond. Specifically, we urge Extension to prioritize the following: 1) mediate the rural/urban dichotomy, 2) tackle structural and institutional power dynamics, and 3) intensify strategies for community resilience.

Offal Wildlife Watching: Connecting Hunters, Wildlife Researchers, And The Public Through Participatory Science, Ellen M. Candler, Grace Milanowski, Amy Rager, Joseph K. Bump

Journal of Extension

Offal Wildlife Watching is a participatory science project that engages deer hunters in wildlife research. The goal of the project is to better understand the ecology of scavenger species that visit deer gut piles across Minnesota. We asked hunters to deploy remote cameras at white-tailed deer gut piles that resulted from their freshly killed deer. Thus far 117 hunters have contributed images and recorded at least 49 different scavenger species. This project is a natural fit in Extension programs and can easily be adapted to other hunted species, biomes, and locations.

Workshop Wine Wednesdays: Change In Behavior Around Local Wines, Aude Watrelot, Sarah Francis, Sarah Al-Mazroa Smith

Journal of Extension

Wine workshops hosted at local wineries positively impacted the behavior of Iowa wine consumers. Many participants (75%) reported visiting an Iowa winery following the workshop. The percentage of participants consuming Iowa wines increased by 20.6% (p

Tailor-Designed Oxalic Acid-Crosslinked Poly(Vinyl Alcohol)/Chitosan Biodegradable Films For Active Food Packaging Applications, Reem Hazem Fathy Anwar Elaasar

Theses and Dissertations

Food packaging plastic waste is posing great threats to ecosystems, human health, and the planet's overall sustainability; therefore, research has been directed towards the fabrication of biodegradable food packaging materials. In this study, active and biodegradable oxalic acid (OA) crosslinked poly(vinyl alcohol) (PVA)/chitosan (CS) composite films of different weight ratios were synthesized via the solvent casting technique and their food packaging properties were investigated. Owing to its water resistance and antibacterial superiority, the composite film with the ratio of OA chemically crosslinked PVA/CS 80:20 was elected to be coated with green tea extract (GTE) to ameliorate its antibacterial and antioxidant …

Understanding The Accuracy And Best Use Of The Woodland Stick As A Tree Height And Diameter Measurement Tool: A Case Study In Oregon, Francisca Belart, Lauren Grand, Alicia Christiansen

Journal of Extension

We conducted a study to assess the accuracy of the Woodland Stick in determining tree diameter and height as compared to professional measurements and how bias can affect tree volume calculation. After comparing professional measurements with five non-professionals (NP’s) using the Woodland Stick, we found that three out of five NP’s had significant differences in mean dbh, and all of the NP’s had significant differences in mean total height. The overall bias for dbh across users was +0.5 inches and +5.1 ft for height. These deviations can result in volume differences between 0 and 30 bf on a single tree.

Leveraging Community Partnerships And Resources To Address Food Insecurity In A Rural County During The Covid-19 Pandemic, Kristen Johnson, Amy Elizer, Karen L. Franck, Soghra Jarvandi

Journal of Extension

Extension professionals are skilled at connecting with community partners and community members to identify needs and address challenges. This paper outlines how Extension professionals quickly responded to address rising rates of food insecurity in a rural Tennessee County during the COVID-19 pandemic. Through an extensive network of state, regional, and local partners, Extension professionals and partners planned and implemented mobile food distributions through the United States Department of Agriculture’s Farmers to Families program. These Extension-led efforts resulted in sustainable, community-driven initiatives to improve food insecurity. Lessons learned can help Extension professionals respond to emerging needs.

Promoting Equity: Embracing Food Justice In The Transformation Of The Food Systems For Bipoc Communities, Frank Yeboah Adusei

Journal of Extension

The food justice movement addresses systemic food inequities impacting BIPOC communities, rooted in civil rights and environmental justice movements. It advocates for fair access to culturally relevant food, combating food deserts, and promoting food sovereignty. Recognizing intersectionality, it emphasizes collaboration to dismantle systemic barriers. This study aims to understand BIPOC communities' challenges and effective strategies, stressing the importance of collaborative efforts for lasting systemic change. Extension professionals face challenges but can contribute through equity-focused strategies, community engagement, and partnerships, advancing food justice and fostering inclusive food systems.

Beer And Biology, A Perfect Pairing: Using Beer To Spread Awareness About Invasive Species, Samuel Nutile, Adam Simpson, Sara Stahlman, Lynne E. Beaty

Journal of Extension

Managing the spread of introduced invasive species relies on educating recreationists that currently or potentially harbor such species. By partnering with a local brewery, we developed the Lagers for Lakes Draft Series to facilitate outreach about aquatic invasive species in Erie, Pennsylvania. To date, Lagers for Lakes drafts and associated launch events have reached over 230 individuals and increased awareness of invasive species by 86%. With the materials provided here and the lessons learned from these events, we hope similar outreach efforts can be developed elsewhere to educate and minimize the spread of invasive species.

Single-Cell Rna Sequencing Identifies Molecular Biomarkers Predicting Late Progression To Cdk4/6 Inhibition In Patients With Hr+/Her2- Metastatic Breast Cancer, Linjie Luo, Peng Yang, Sofia Mastoraki, Xiayu Rao, Yan Wang, Akshara Singareeka Raghavendra, Senthil Damodaran, Kelly K Hunt, Jing Wang, Ziyi Li, Khandan Keyomarsi

Faculty, Staff and Student Publications

Background: Cyclin-dependent kinase 4/6 inhibitors (CDK4/6is) in combination with endocrine therapy are the standard treatment for patients with hormone receptor-positive, HER2-negative metastatic breast cancer (mBC). Despite the efficacy of CDK4/6is, intrinsic resistance occurs in approximately one-third of patients, highlighting the need for reliable predictive biomarkers.

Methods: Single-cell RNA sequencing analyzed metastatic tumors from HR+/HER2- mBC patients pre-CDK4/6i treatment at baseline (BL) and/or at disease progression. BL samples were from CDK4/6i responders (median progression-free survival [mPFS] = 25.5 months), while progressors were categorized as early-progressors (EP, mPFS = 3 months) and late-progressors (LP, mPFS = 11 months). Metastatic sites included liver, …

Identification And Functional Characterization Of The Icse1 Gene From Ilex Cornuta, Xiaoxi Zhang, Li Zhu, Weiwei Zhang, Feng Xu, Jiabao Ye, Yongling Liao

Turkish Journal of Agriculture and Forestry

Ilex cornuta (I. cornuta) is a traditional Chinese medicine that is rich in various secondary metabolites with pharmacological activities, among which triterpenoid saponins are one of the main active components. Squalene epoxidase (SE) is a key regulatory enzyme in the biosynthesis of triterpenoids. In this study, we isolated a squalene epoxidase (IcSE1) from I. cornuta and analyzed its molecular characteristics and functions. The results indicated that the total length of the IcSE1 sequence was 1942 bp, which includes a 1638 bp open reading frame (ORF) that encodes 545 amino acids. The theoretical isoelectric point (pI) and molecular weight of the …

A Genome-Wide Association Study To Detect Candidate Genes Controlling Iron And Manganese Accumulation In Walnut (Juglans Regia L.), Özge Doğanay, Erdal Orman, Bülent Yağmur, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş

Turkish Journal of Agriculture and Forestry

The walnut is one of the important low-glycemic sources of vegetable protein that benefit human health. Iron (Fe) and Manganese (Mn) are two of the walnut’s important nutritional components. This study aimed to identify single-nucleotide polymorphism (SNP) markers associated with the concentrations of Mn and Fe in walnut kernels and the genetic variation in these concentrations. The population was evaluated for two different years using 154 walnut genotypes and genotyping was performed with 16,473 SNP markers. A genome-wide association study was performed on 154 genotypes. A total of five and 15 SNPs were identified in different walnut genotypes, which were …

Regression Modeling Of Cumulative Incidence Function For Left-Truncated Right-Censored Competing Risks Data: A Modified Pseudo-Observation Approach, Rong Rong, Jing Ning, Hong Zhu

Faculty, Staff and Student Publications

Statistical methods have been developed for regression modeling of the cumulative incidence function (CIF) given left-truncated right-censored competing risks data. Nevertheless, existing methods typically involve complicated weighted estimating equations or nonparametric conditional likelihood function and often require a restrictive assumption that censoring and/or truncation times are independent of failure time. The pseudo-observation (PO) approach has been used in regression modeling of CIF for right-censored competing risks data under covariate-independent censoring or covariate-dependent censoring. We extend this approach to left-truncated right-censored competing risks data and propose to directly model the CIF based on POs, under general truncation and censoring mechanisms. We …

Bbox1 Restrains Tbk1-Mtorc1 Oncogenic Signaling In Clear Cell Renal Cell Carcinoma, Chengheng Liao, Lianxin Hu, Liwei Jia, Jin Zhou, Tao Wang, Hua Zhong, Lei Dong, Lei Guo, Qian Liang, Cheng Zhang, Fangzhou Zhao, Jun Fang, Hongyi Liu, Shina Li, Lin Xu, Jeremy M Simon, Srinivas Malladi, Payal Kapur, James Brugarolas, Ralph J Deberardinis, Qing Zhang

Faculty, Staff and Student Publications

Clear cell renal cell carcinoma (ccRCC), a metabolic disease originating from renal proximal convoluted tubule (PCT) epithelial cells, remains incompletely understood in terms of its initiating signaling events. Here, we identify γ-butyrobetaine hydroxylase 1 (BBOX1), a key enzyme in carnitine synthesis predominantly expressed in PCT cells, as a tumor suppressor in ccRCC. BBOX1 expression is lost during ccRCC malignant transformation, and its restoration reduces cell viability in physiological medium and inhibits xenograft tumor growth. Transcriptomic analyses reveal that BBOX1 suppresses critical metabolic pathways including mTORC1 signaling and glycolysis in ccRCC. Further, we identify TANK-binding kinase 1 (TBK1) as an essential …

A Fire Deficit Persists Across Diverse North American Forests Despite Recent Increases In Area Burned, Sean A. Parks, Christopher H. Guiterman, Ellis Q. Margolis, Margaret Lonergan, Ellen Whitman, John T. Abatzoglou, Donald A. Falk, James D. Johnston, Lori D. Daniels, Charles W. Lafon, Rachel A. Loehman, Kurt F. Kipfmueller, Cameron E. Naficy, Marc-André Parisien, Jeanne Portier, Michael C. Stambaugh, A. Park Williams, Andreas P. Wion, Larissa L. Yocom

Wildland Resources Faculty Publications

Rapid increases in wildfire area burned across North American forests pose novel challenges for managers and society. Increasing area burned raises questions about whether, and to what degree, contemporary fire regimes (1984–2022) are still departed from historical fire regimes (pre-1880). We use the North American tree-ring fire-scar network (NAFSN), a multi-century record comprising > 1800 fire-scar sites spanning diverse forest types, and contemporary fire perimeters to ask whether there is a contemporary fire surplus or fire deficit, and whether recent fire years are unprecedented relative to historical fire regimes. Our results indicate, despite increasing area burned in recent decades, that a …

Structural, Molecular And Developmental Evidence For Cell-Type Diversity In Cnidarian Mechanosensory Neurons, Julia Baranyk, Kristen Malir, Miguel A.P. Silva, Sakura Rieck, Gracie Scheve, Nagayasu Nakanishi

Biological Sciences Faculty Publications and Presentations

Deploying a conserved mechanosensory neuron known as the concentric hair cell, cnidarians have evolved diverse mechanoreceptors from hydroid filiform tentacles to jellyfish statocysts. However, it is unknown whether cnidarian mechanoreceptor evolution has relied solely on repurposing a single ancestral mechanosensory neuron type. Here we report evidence for cell-type diversity of mechanosensory neurons in sea-anemone cnidarian Nematostella vectensis. Uncovered in the ectoderm of feeding tentacles are conventional type I hair cells and previously unrecognized type II hair cells differing in the structure of apical sensory apparatus and synapses. Moreover, we identify TRP channel-encoding gene polycystin-1 as a type-II-hair-cell-specific essential mediator of …

A Deep Learning-Based System For Automatic Detection Of Emesis With High Accuracy In Suncus Murinus, Zengbing Lu, Yimeng Qiao, Xiaofei Huang, Dexuan Cui, Julia Y H Liu, Man Piu Ngan, Luping Liu, Zhixin Huang, Zi-Tong Li, Lingqing Yang, Aleena Khalid, Yingyi Deng, Sze Wa Chan, Longlong Tu, John A Rudd

Children’s Nutrition Research Center Staff Publications

Quantifying emesis in Suncus murinus (S. murinus) has traditionally relied on direct observation or reviewing recorded behaviour, which are laborious, time-consuming processes that are susceptible to operator error. With rapid advancements in deep learning, automated animal behaviour quantification tools with high accuracy have emerged. In this study, we pioneere the use of both three-dimensional convolutional neural networks and self-attention mechanisms to develop the Automatic Emesis Detection (AED) tool for the quantification of emesis in S. murinus, achieving an overall accuracy of 98.92%. Specifically, we use motion-induced emesis videos as training datasets, with validation results demonstrating an accuracy of 99.42% for …

Atsubp-2.0: An Integrated Web Server For The Annotation Of Arabidopsis Proteome Subcellular Localization Using Deep Learning, Naveen Duhan, Rakesh Kaundal

Plants, Soils and Climate Student Research

The organization of subcellular components in a cell is critical for its function and studying cellular processes, protein–protein interactions, identifying potential drug targets, network analysis, and other systems biology mechanisms. Determining protein localization experimentally is time-consuming and expensive. Due to the need for meticulous experimentation, validation, and data analysis, computational methods provide a quick and accurate alternative. Arabidopsis thaliana, a beneficial model organism in plant biology, facilitates experimentation and applies to other plants. Predicting its proteins' subcellular localization can improve our understanding of cellular processes and have applications in crop improvement and biotechnology. We propose AtSubP-2.0, an extension of …