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Omega-3 Fatty Acids, Kristina Krupa, Kristina Fritz, Mayur S. Parmar

HPD Articles

No abstract provided.

Ecological Features Of Cyanobacteria And Algae Communities Of The Littoral Of The Meromictic Lake Shira (Khakassia Republic, Russia), Lira Gaysina, A I. Fazlutdinova, O N. Mukhina, L F. Akhmadeyeva, D Yu Rogozin, A O. Bul’Khin, N V. Sukhanova, Jeffrey R. Johansen

2020 Faculty Bibliography

During the study of cyanobacteria and algae from littoral of the meromictic Lake Shira (Republic of Khakas- sia, Russia) forty-eight taxa were identified: Cyanobacteria – 7, Chlorophyta – 5, and Bacillariophyta – 36 species. Cyanobacteria were represented by cosmopolitan taxa Leptolyngbya voronichiniana, cf. Trichocoleus hospitus and widely distributed in the freshwater ecosystems species Phormidium cf. paulsenianum, Pseudophormidium cf. golenkinianum, P. pauciramosum, Leptolyngbya perforans, and L. subtilissima. Among green algae typical freshwater taxa Chloroidium saccharophilum, Desmodesmus abundans, Oocystis lacustris, Chlorella sp., Stigeoclonium sp. were detected. Among Bacillariophyta the most frequent species were Amphora ovalis, Cymbella affinis, Encyonema silesiacum, Eunotia fallax, Gomphonella …

Distribution, Density, Movement, And Support For Management Of Mountain Sucker, Pantosteus Jordani, In The Black Hills Of South Dakota, Seth J. Fopma

Electronic Theses and Dissertations

Mountain Sucker, Pantosteus jordani, is a cold-water species native to the Intermountain West. Fringe populations of Mountain Sucker have experienced declines in recent decades. The population of Mountain Sucker found in the Black Hills of South Dakota represents the eastern fringe of the species’ native range. Recognized as both an indicator of biologic health and as a species of greatest conservation need in South Dakota, recent studies have suggested significant declines in both distribution and abundance. Despite the recent study of Mountain Sucker in the region, increased understanding of Mountain Sucker ecology is needed to effectively manage for the conservation …

Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong

Electronic Theses and Dissertations

Transposable elements, also called jumping genes, comprise almost 45% of the human genome. In contrast, only 1% of the human genome is protein-coding sequences. The function and advantages of maintaining such massive copies of transposable elements in the human genome are still unclear. Long interspersed element 1 (L1), the most substantial group and the only active autonomous transposable element in the human genome, has revealed its unique roles in many diseases. The insertional mutagenesis induced by L1 retrotransposition events could threaten human genomic stability and generate unexpected mutations. L1 overexpression has been documented in both somatic and germline cells and, …

What's In My Histological Dyes Anyway?, Ashley Post

Undergraduate Honors Thesis Collection

There are many different dyes that are used for biological research, but not much is commonly known about the origins of the dyes or how they are synthesized. Our dyes of interest were Coomassie Blue G and Rhodamine B. Coomassie Blue G is a blue colored dye used mainly for SDS-PAGE (gel electrophoresis) in biological research. Rhodamine B is used for its ability to fluoresce under ultraviolet light. Both of these dyes have been used for research purposes for many years despite researchers who use the dyes not having little background knowledge regarding the purity of the manufactured dyes. We …

Chemical Composition And Antimicrobial And Antioxidant Activity Of Helichrysum Italicum (Roth) G.Don Subspecies Essential Oils, Silviya Mollova, Hafize Fidan, Daniela Antonova, Dimitar Bozhilov, Stanko Stanev, Iliana Kostova, Albena Stoyanova

Turkish Journal of Agriculture and Forestry

The chemical composition and the antimicrobial and antioxidant activity of essential oils from two Helichrysum italicum (Roth) G.Don subspecies grown in Bulgaria were investigated. As a result, 95 compounds with concentrations above 0.05% were detected by GC/MS and 46 of them, mainly mono- and sesquiterpenes, representing 79.81% and 85.51% of the total content of the samples, were identified. The main constituents of the essential oil from Helichrysum italicum subsp. microphyllum (plant origin from Bosnia) were monoterpene α-pinene (20.84%) and sesquiterpene γ-curcumene (16.53%), followed by β-selinene (5.59%), ar-curcumene (4.39%), trans-caryophyllene (4.35%), β-diketone italidione I (4.32%), α-selinene (4.28%), and neryl acetate (3.81%). …

Hormonal Regulation Of Glycine Decarboxylase And Its Metabolic Outcomes, Ruta Milind Jog

Wayne State University Dissertations

The amino acid glycine is involved in generation of multiple critical metabolites including glutathione, heme, and creatinine. Interestingly, in both humans and rodents, circulating glycine levels are significantly reduced in obesity, glucose intolerance, type II diabetes and non-alcoholic fatty liver disease. The glycine cleavage system is the predominant glycine degradation pathway in humans. The rate-limiting enzyme of glycine cleavage system is glycine decarboxylase (GLDC), and loss-of-function mutations of GLDC cause hyperglycinemia. Here, we show that GLDC gene expression is upregulated in livers of mouse models of diabetes and diet-induced obesity as well as in the fasted state in normal animals. …

Life History Metrics Of Wahoo (Acanthocybium Solandri) From The Northern Gulf Of Mexico, Erik T. Lang, Chris A. Levron, Cijii D. Marshall, Brett J. Falterman

Gulf and Caribbean Research

Wahoo (Acanthocybium solandri) are highly sought in recreational and commercial fisheries in the Gulf of Mexico (GOM), yet the harvest of Wahoo is currently unmanaged in the GOM region outside of Florida waters. Life history metrics are critical to stock assessments and science-based management. Between 2014-2017, Louisiana Department of Wildlife and Fisheries (LDWF) biologists collected Wahoo otoliths and ovaries (n=198) from the northern GOM Wahoo recreational fishery based in Venice, LA to estimate life history metrics of age, growth, and maturity. A protocol was developed to section and age the small, fragile otoliths. A 2-parameter Von Bertalanffy growth …

Methods To Integrate Genetic And Clinical Data For Disease Subtyping, Diana Mabel Diaz-Herrera

Wayne State University Dissertations

Enormous efforts have been made to collect genetic and clinical data from cancer patients to advance the understanding of disease development and progression. Processing and analyzing these flows of data is challenging. Many computational methods have been proposed to help different fronts of biology and medicine. The integration of clinical and genetic data using computational methods towards personalized medicine is considered the future for oncology studies, and this thesis contributes in this direction. This thesis presents three new data integration approaches to elucidate granular and meaningful disease sub-types from high-dimensional complex genetic and clinical variables, which is an essential step …

The Role Of Genotype In Saccharomyces Cerevisiae Resistance To 4-Methylcyclohexanemethanol, Michael C. Ayers

Graduate Theses, Dissertations, and Problem Reports (ETD)

The chemical 4-methylcyclohexanemethanol (MCHM) spilled in the Elk River near Charleston, WV in January 2014, impacting the drinking water of 300,000 residents. Initial studies on MCHM following the spill attempted to characterize its toxicological effects in animals, but largely ignored characterization of its effects on cellular pathways and metabolism. In this study, I used this chemical as a novel stressor for the model eukaryote S. cerevisiae to explore the relationship of genotype and stress phenotypes. Initial exploration characterized the stress phenotype of MCHM in yeast through growth assays, cell cycle analysis, and transcriptomic data. MCHM exposure caused cells to arrest …

Protein/Peptide Characterization Using Mass Spectrometry And Molecular Dynamics Simulations, Ahmad Kiani Karanji

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mass spectrometry (MS) based-techniques and molecular dynamics (MD) simulations have been used to characterize protein/peptide structure as well as their interactions with lipid vesicles and detergents. Chapter 1 introduces an introduction to the concepts and tools that were used in this work. In Chapter 2, the dominant gas-phase conformer of [M+3H]3+ ions of the model peptide Acetyl-PSSSSKSSSSKSSSSKSSSSK are examined with ion mobility spectrometry (IMS), gas-phase hydrogen deuterium exchange (HDX), and mass spectrometry (MS) techniques. This section furthers the development of a protein structural prediction tool by providing information about gas-phase ion conformers of two model peptides having different solution conformational …

Biological And Computational Studies Of The Structure And Function Of Pul103, A Human Cytomegalovirus Tegument Protein, Ashley N. Anderson

Wayne State University Dissertations

Human cytomegalovirus (HCMV) is an enveloped, single segment, double-stranded DNA virus. HCMV infection causes disease in immunocompromised (HIV patients, transplant recipients) and immunodeficient (fetuses, neonates) populations. Current treatments are effective but are either limited in use or can lead to organ damage and/or antiviral resistance, and no vaccines are available. Additional antiviral targets are needed. HCMV pUL103 is a potential antiviral target. pUL103 is a conserved herpesvirus protein present in the tegument, layer of proteins and RNA between the envelope and capsid of HCMV virions. pUL103 helps reorganize cellular secretory machinery (Golgi, endosomes) to form the cytoplasmic virion assembly compartment …

Controlled Release Of Fibroin Repeat-Tagged Proteins From Silk Hydrogel, Jaturong Promsuk

Chulalongkorn University Theses and Dissertations (Chula ETD)

Protein-based biologic drugs such as growth factors are playing important roles in modern medicine. These proteins can be used to stimulate local tissue repair. Growth factors are often incorporated into a scaffold, such as a hydrogel, and then put into the desired area of the body to achieve therapeutic effects. However, diffusion of these growth factors outside of the designated area or inappropriate release time can have deleterious consequences, such as inducing tumors in other areas of the body or uncontrolled inflammation. Therefore, this project aims to create a model to study diffusion of protein from hydrogel by using green …

Novel Hiv Adaptation Strategy Against Host Cd8 T Cell Immunity, Kai Qin

All ETDs from UAB

Since the 1980s, HIV infection has caused millions of death and tremendous economic losses worldwide. Despite great scientific advances, the only effective therapy to date, antiretroviral therapy (ART), merely delays disease progression yet cannot eradicate viral infection. Therefore, an effective HIV vaccine that can prevent the acquisition of infection would be a desirable alternative. The critical role of CD8 T-cell immunity in HIV infection has been well documented and therefore not surprisingly a great deal of effort has been focused on developing an effective CD8 T-cell based HIV vaccine. However, by far, such CD8 T-cell based HIV vaccine trials have …

Effects Of Dietary Guar Meal With Or Without Beta-Mannanase On Performance And Eggquality Traits In Laying Hens, Süleyman Çalişlar

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of beta-mannanase supplementation to laying hens? diets with different guar meal (GM) ratios on performance and egg quality traits were investigated. The experimental period lasted 126 days. Ninety-six Lohman Brown hens at the age of 56 weeks were kept in individual cages. They were divided into 8 treatment groups as follows: 0% guar meal (GM0) (control); 0% GM + 0.05% beta-mannanase (GM0E); 8% GM (GM8); 8% GM + 0.05% beta-mannanase (GM8E); 16% GM (GM16); 16% GM + 0.05% beta-mannanase (GM16E); 24% GM (GM24); and 24% GM + 0.05% beta-mannanase (GM24E). A photoperiod of 16.5 h …

Comparative Morphological Analysis Of Diploid And Triploid Oysters, Crassostrea Gigas,Farmed In The Black Sea, Oksana Yurievna Vialova

Turkish Journal of Veterinary & Animal Sciences

Triploid organisms, in particular oysters, are widely used in marine aquaculture. However, the advantage of triploid oysters compared to diploids has not been proven in all cases. Comparative morphological analysis of Pacific oysters Crassostrea gigas of different ploidy levels allows evaluating the benefits of raising triploids in the Black Sea. First, the morphological characteristics of diploid and triploid Pacific oysters C. gigas farmed in the Black Sea were studied. Allometric ratios of weight (W, g) and shell height (H, mm) of oysters were obtained, which are described by the equations W$_{2n}$ = 4 × 10$^{-4}$ × H$_{2n}$ $^{2.56}$, R2 = …

Gulls As Sources Of Environmental Contamination By Colistin-Resistant Bacteria, Alan B. Franklin, Andrew M. Ramey, Kevin T. Bentler, Nicole L. Barrett, Loredana M. Mccurdy, Christina A. Ahlstrom, Jonas Bonnedahl, Susan A. Shriner, Jeffrey C. Chandler

United States Department of Agriculture Wildlife Services: Staff Publications

In 2015, the mcr-1 gene was discovered in Escherichia coli in domestic swine in China that conferred resistance to colistin, an antibiotic of last resort used in treating multi-drug resistant bacterial infections in humans. Since then, mcr-1 was found in other human and animal populations, including wild gulls. Because gulls could disseminate the mcr-1 gene, we conducted an experiment to assess whether gulls are readily colonized with mcr-1 positive E. coli, their shedding patterns, transmission among conspecifics, and environmental deposition. Shedding of mcr-1 E. coli by small gull flocks followed a lognormal curve and gulls shed one strain >101 …

Validation Of A Screening Method For The Detection Of Colistin-Resistant E. Coli Containing Mcr-1 In Feral Swine Feces, Jeffrey C. Chandler, Alan B. Franklin, S. N. Bevins, Kevin T. Bentler, Jonas Bonnedahl, Christina A. Ahlstrom, Bledar Bisha, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

A method was developed and validated for the detection of colistin-resistant Escherichia coli containing mcr-1 in the feces of feral swine. Following optimization of an enrichment method using EC broth supplemented with colistin (1 μg/mL) and vancomycin (8 μg/mL), aliquots derived from 100 feral swine fecal samples were spiked with of one of five different mcr-1 positive E. coli strains (between 10⁰ and 10⁴ CFU/g), for a total of 1110 samples tested. Enrichments were then screened using a simple boil-prep and a previously developed real-time PCR assay for mcr-1 detection. The sensitivity of the method was determined in …

Specimens Of Opportunity Reveal Novel Information On The Sowerby's Beaked Whale (Mesoplodon Bidens), Kerri Jean Smith

Open Access Theses & Dissertations

Conservation science requires quickly acquiring information and taking action in order to protect species at risk of extinction. Elusive wildlife, however, present challenges to effective conservation measures because it is often difficult to collect enough data on these species to recognize their conservation needs and implement management plans. As a result, researchers must identify alternative means of gathering sufficient data on these species. Specimens of opportunity, such as museum specimens, provide a way to improve knowledge on these species, and these specimens have already proven valuable by increasing information on biodiversity, habitat and range, and population structure in many species. …

Polygenic Risk Score Analysis Revealed Shared Genetic Background In Attention Deficit Hyperactivity Disorder And Narcolepsy, Nagahide Takahashi, Tomoko Nishimura, Taeko Harada, Akemi Okumura, Damee Choi, Toshiki Iwabuchi, Hitoshi Kuwabara, Shu Takagi, Yoko Nomura, Jeffrey H. Newcorn, Nori Takei, Kenji J. Tsuchiya

Publications and Research

Attention deficit hyperactive disorder (ADHD) is a highly heritable neurodevelopmental disorder, and excessive daytime sleepiness is frequently observed in ADHD patients. Excessive daytime sleepiness is also a core symptom of narcolepsy and essential hypersomnia (EHS), which are also heritable conditions. Psychostimulants are effective for the symptomatic control of ADHD (primary recommended intervention) and the two sleep disorders (frequent off-label use). However, the common biological mechanism for these disorders has not been well understood. Using a previously collected genome-wide association study of narcolepsy and EHS, we calculated polygenic risk scores (PRS) for each individual. We investigated a possible genetic association between …

Amyloid Proteins And Fibrils Stability, Farbod Mahmoudinobar

Dissertations

Compared to globular proteins that have a stable native structure, intrinsically disordered peptides (IDP) sample an ensemble of structures without folding into a native conformation.One example of IDP is the amyloid-beta(Abeta) protein which is the main constituent of senile plaques in the brain of Alzheimer's patients.Understanding the process by which IDPs undergo structural changes to form oligomers that eventually aggregate into senile plaques/amyloid fibrils may significantly advance the development of novel therapeutic methods to treat neurodegenerative diseases, for which there is no cure to date. This dissertation has two main objectives. The first one is to investigate and identify structural …

The Values Of Warm-Season Native Perennial Grasses Grown For Pasture Or Biofuel In The Southern Great Plains, Usa, James K. Rogers, Bryan Nichols, Jon T. Biermacher, Jagadeesh Mosali

IGC Proceedings (1977-2023)

The Renewable Fuel Standard under the Energy Independence and Security Act of 2007 mandated the production of 136 billion liters of cellulosic biofuel by 2022. Switchgrass (Panicum virgatum) has been identified as a primary feedstock because it is a perennial, produces high yields and is adapted to a wide environmental range. Development of the cellulosic biofuel industry has been slow. A reason for this slow development is lack of available feedstock driven by lack of a developed market. Rather than considering switchgrass only as a dedicated biofuel feedstock, we examined its potential both for grazing and biofuel feedstock. …

An Investigation Of The Potentials Of 2-[(2-Hydroxyphenyl) Methylidene] Hydrazine-1-Carbothioamide And Its Mn(Ii) And Zn(Ii) Complexes As Antimicrobial Agents, Umar Sani, Musbahu Yahaya, Dailami S. Adam

Tanzania Journal of Science

Antibiotic resistance as a result of misuse, overuse or abuse of drugs is an issue of serious concern world over. The quest for more active and robust antimicrobial agents remains a scientific challenge and requires multimillion-dollar investment in the area of drug design and development. Here in, we report the synthesis and characterisation of a tridentate thiosemicarbazone (2-[(2-hydroxyphenyl)methylidene]hydrazine-1-carbothioamide) (HL) from salicylaldehyde and thiosemicarbazide. Its complexes with Mn(II) and Zn(II) were prepared following similar technique and characterised by melting point, FT-IR, UV –visible spectrophotometry, and elemental analysis. The Mn(II) complex showed paramagnetism with a magnetic moment value of 5.80 BM while …

Nanomaterials For Biosensing Lipopolysaccharide, Palak Sondhi, Helal Maruf, Keith Stine

Chemistry & Biochemistry Faculty Works

Lipopolysaccharides (LPS) are endotoxins, hazardous and toxic inflammatory stimulators released from the outer membrane of Gram-negative bacteria, and are the major cause of septic shock giving rise to millions of fatal illnesses worldwide. There is an urgent need to identify and detect these molecules selectively and rapidly. Pathogen detection has been done by traditional as well as biosensor-based methods. Nanomaterial based biosensors can assist in achieving these goals and have tremendous potential. The biosensing techniques developed are low-cost, easy to operate, and give a fast response. Due to extremely small size, large surface area, and scope for surface modification, nanomaterials …

A Multivariate Ecological Approach To Delineate Unionid Freshwater Mussel Species, Ava M. Laszlo

Biology Theses

Freshwater mussels represent one of the most imperiled faunal groups on the planet and are critical to freshwater ecosystems. If mussel species are not carefully defined, our conservation plans may miss differences in the habitat needs of different species and the conservation strategies may not be as successful. Because ecological niche models can be used to provide evidence to support similar or dissimilar ecological niches and habitat requirements, I used modelling to forecast suitable habitat for Fusconaia askewi and F. lananensis, two purported threatened Texas species. The modeling results indicated that these species are not ecologically different. Based upon …

Wildlife Survey Of National Parks To Assess Reptilian Biodiversity, Ajk, Jibran Haider, Inayatullah Malik, Sabiha Shamim

Journal of Bioresource Management

The class Reptilia belongs to phylum Chordata. This group forms a large community of the land vertebrates. However, they remain relatively under-examined. Roll et al. (2017) studied the global distribution of more than 10,000 reptiles. Nearly194 reptilian species have been reported from Pakistan (WWF, n.d.). Five protected parks were studied from February 2008 to May 2010. Dhirkot Nature Reserve (DNR), Banjosa Nature Reserve (BNR), Tolipir National Park (TNP), Pir Chanasi National Park (PCNP) and Pir Lasura National Park (PLNP) were considered. Fifteen species of reptiles were observed in TNP. Ten species of reptiles were recorded from PCNP. Ten species of …

Cpue Standardization For The Pacific Saury (Cololabis Saira) Fishery In The Northwest Pacific, Wen-Bin Huang, Chih-Hao Hsieh, Tai-Sheng Chiu, Wei-Ting Hsu, Chih-Shin Chen

Journal of Marine Science and Technology–Taiwan

In stock assessment and fisheries management, the standardization of catch per unit effort (CPUE) is critical to derive an effective index of the abundance of exploited fish stocks. In addition to developing various statistical models, a method including the relative fishing power (RFP) of individual vessels has been developed from an intuition concept and applied to bottom trawl fisheries using logbookbased data. This is a convenient and traightforward approach for standardizing the catch-effort data of a fishery. The Pacific saury is a pelagic fish species, and its stock in the Northwest Pacific has been targeted by international fleets since the …

Survival And Development Of Striacosta Albicosta (Smith) (Lepidoptera: Noctuidae) Immature Stages On Dry Beans, Non-Bt, Cry1f, And Vip3a Maize, Débora G. Montezano, Thomas E. Hunt, Alexandre Specht, Priscila C. Luz, Julie A. Peterson

Department of Entomology: Faculty Publications

Striacosta albicosta is a crop pest that causes economic damage in the United States and Canada. Only maize and dry beans are shown to be suitable hosts, since larval development is incomplete on other hosts. The objective of this study was to describe the developmental parameters of immature stages of S. albicosta feeding on dry beans, non-Bt, Cry1F, and Vip3A maize. For Vip3A, mortality was 100% after 24 h. Larvae feeding on non-Bt maize had the highest larval survival (70.6%) compared to the other hosts. Maize expressing Cry1F had higher survival (31.3%) than dry beans (26.0%). Larvae feeding on dry …

9th Annual Postdoctoral Science Symposium, University Of Texas Md Anderson Cancer Center Postdoctoral Association

Annual Postdoctoral Science Symposium Abstracts

The mission of the Annual Postdoctoral Science Symposium (APSS) is to provide a platform for talented postdoctoral fellows throughout the Texas Medical Center to present their work to a wider audience. The MD Anderson Postdoctoral Association convened its inaugural Annual Postdoctoral Science Symposium (APSS) on August 4, 2011.

The APSS provides a professional venue for postdoctoral scientists to develop, clarify, and refine their research as a result of formal reviews and critiques of faculty and other postdoctoral scientists. Additionally, attendees discuss current research on a broad range of subjects while promoting academic interactions and enrichment and developing new collaborations.

Implementing And Evaluating A Course-Based Undergraduate Research Experience (Cure) At A Hispanic-Serving Institution, Michele I. Shuster Phd, Jennifer Curtiss, Timothy F. Wright, Camilla Champion, Maryam Sharifi, Judith Bosland

Interdisciplinary Journal of Problem-Based Learning

We are examining the impact of a course-based undergraduate research experience (CURE) at a land-grant, Hispanic-serving institution in the southwestern United States. Students in our CURE completed one or two extended research projects over a single semester. Our CURE enrolled a high proportion of underrepresented minority students (70.3%), including 60.2% Hispanic students. One year after CURE completion, 31.5% of CURE students had graduated with a STEM degree, and 54.3% were enrolled in a STEM major. Pre- and postcourse surveys of indicators of persistence including scientific self-efficacy, scientific identity, valuing scientific community objectives, and intention to persist showed positive shifts. Impacts …