Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 1141 - 1170 of 1423

Full-Text Articles in Entire DC Network

Weevil Management In Orchards And Vineyards Looks Promising, Stewart Learmonth Jan 2000

Weevil Management In Orchards And Vineyards Looks Promising, Stewart Learmonth

Journal of the Department of Agriculture, Western Australia, Series 4

Research and monitoring undertaken by Agriculture Western Australia is showing that the management of weevils in Western Australia's orchard crops and vineyards is improving. A number of alternative management strategies are being implemented, and future research will assess the effectiveness of non-chemical approaches to weevil management.


Designing And Conducting Experiments For Range Beef Cows, Don C. Adams, Merlyn K. Nielsen, Walter H. Schacht, Richard T. Clark Jan 2000

Designing And Conducting Experiments For Range Beef Cows, Don C. Adams, Merlyn K. Nielsen, Walter H. Schacht, Richard T. Clark

Department of Animal Science: Faculty Publications

Designing and conducting effective research for range beef systems involves analysis of intended application of the results, identification of factors affecting variation, and selection of appropriate research methods so that precise inferences can be made. Variances associated with time, location, animal, and error in grazing research can be high. Variation due to treatment × location and treatment × time interactions is reduced by increasing the number of locations and periods tested. Random error is reduced by increasing the total number of observations. Animals, pastures, and weather are significant sources of variation in grazing studies. Factors that influence nutrient requirements or …


Geographic Variations And Taxonomic Status Of Miniopterus Schreibersi (Kuhl, 1819) In Turkey (Chiroptera: Vespertilionidae), İrfan Albayrak, Şule Coşkun Jan 2000

Geographic Variations And Taxonomic Status Of Miniopterus Schreibersi (Kuhl, 1819) In Turkey (Chiroptera: Vespertilionidae), İrfan Albayrak, Şule Coşkun

Turkish Journal of Zoology

This study is a taxonomical revision of 257 specimens of Miniopterus schreibersi (Kuhl, 1819) collected from Turkey between 1974 and 1994. A total of 30 external and cranial measurements and weights and the ages of the specimens were determined. Diagnostic characters, habitat, and measurements of subspecies were recorded. The specimens collected in Turkey were evaluated by comparison with the nominate form distributed nearby, Miniopterus schreibersi pallidus Thomas, 1907, and synonyms. It was determined that both the nominate form and Miniopterus schreibersi pallidus exist in Turkey.


Remote Sensing Observations Of Winter Phytoplankton Blooms Southwest Of The Luzon Strait In The South China Sea, Dan-Ling Tang, I-Hsun Ni, Dana R. Kester, Frank E. Muller-Karger Dec 1999

Remote Sensing Observations Of Winter Phytoplankton Blooms Southwest Of The Luzon Strait In The South China Sea, Dan-Ling Tang, I-Hsun Ni, Dana R. Kester, Frank E. Muller-Karger

Marine Science Faculty Publications

The Luzon Strait is a channel between the Philippine Sea and the South China Sea. This area is traditionally classified as an oligotrophic zone with low primary productivity. Even so, high concentrations of pigment were detected 100 km southwest of the Strait through analysis of historical Coastal Zone Color Scanner (CZCS) data that the Nimbus-7 satellite collected during the winters of 1979 to 1986. These blooms were observed in December 1979, February 1983, February 1985, and January 1986, when sea surface temperatures measured with the National Oceanic and Atmospheric Administration's (NOAAs) Advanced Very High Resolution Radiometer (AVHRR) were 23 to …


A Study Of The Seasonal Composition And Abundance Of Phytoplankton And Autotrophic Picoplankton In A Brackish Water Lake In Portsmouth Virginia, Jennifer Leigh Wolny Oct 1999

A Study Of The Seasonal Composition And Abundance Of Phytoplankton And Autotrophic Picoplankton In A Brackish Water Lake In Portsmouth Virginia, Jennifer Leigh Wolny

Biological Sciences Theses & Dissertations

The phytoplankton and autotrophic picoplankton populations of Hoffler Lake, a brackish-water lake in Portsmouth, Virginia, were monitored from May 1997 through May 1998, Analyses of the phytoplankton community using the Utennohl method showed a dominance of Chlorophytes (61-88% of the total abundance) throughout the year, including a winter bloom of Chlamydomonas snowii (maximum concentration of 2.5 x 101 cells/L). Subdominants were Cyanobacteria (10-33% of the total abundance) whose composition included several species of Anabaena, Lyngbya, and a fall bloom of Microcoleus sp. Diatoms, dinoflagellates, and cryptophytes played a secondary role in the phytoplankton community of Hoffler Lake. …


Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi Jun 1999

Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi

Marine & Environmental Sciences Faculty Articles

Sponges are well known to harbor large numbers of heterotrophic microbes within their mesohyl. Studies to determine the diversity of these associated microbes have been attempted for only a few shallow water species. We cultured various microorganisms from several species of Discodermia collected from deep water using the 'Johnson-Sea-Link' manned submersibles, and characterised them by standard microbiological identification methods. Characterisation of a small proportion (ca. 10%) of the total and potential eubacterial isolate collection with molecular systematics techniques revealed a wide diversity of microbes. Phylogenetic analyses of 32 small subunit (SSU) 16S-like rRNA gene sequences from different micorbes indicated high …


Species: Turning A Conundrum Into A Research Program, Daniel R. Brooks, Deborah A. Mclennan, E. C. Bernard Jun 1999

Species: Turning A Conundrum Into A Research Program, Daniel R. Brooks, Deborah A. Mclennan, E. C. Bernard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The most appropriate ontological basis for understanding the role of species in evolutionary biology is the Evolutionary Species Concept. The ESC is not an operational concept, but one version of the Phylogenetic Species Concept is. Linking the ontology of species with the epistemological basis of actual biological studies requires that we specify both a discovery mode for identifying collections of organisms that we believe are evolutionary species, and a series of evaluation criteria for assessing those entities we have discovered. Simply naming a collection of specimens, no matter how strong one’s evolutionary beliefs, is not sufficient for declaring that evolutionary …


Strength Properties Of Juvenile And Mature Wood Of Twelve Families Of Loblolly Pine (Pinus Taeda L.), Elizabeth Lynn Forbes Apr 1999

Strength Properties Of Juvenile And Mature Wood Of Twelve Families Of Loblolly Pine (Pinus Taeda L.), Elizabeth Lynn Forbes

Electronic Theses and Dissertations

The wood mechanical properties of progeny from twelve open-pollinated parent trees were examined. Six families were from parents which had high wood specific gravity and six were from parents which had low wood specific gravity. Thirty-two-year-old trees, that had been planted at Many, Louisiana, were sampled and juvenile and mature wood test specimens were prepared. Maximum crushing strength, modulus of rupture, modulus of elasticity and wood specific gravity were determined from compression parallel to the grain tests and static bending tests. Maximum crushing strength, modulus of rupture, modulus of elasticity, and specific gravity were found to vary significantly between family …


Phylogenetic Relationships In Nuphar (Nymphaeaceae): Evidence From Morphology, Chloroplast Dna, And Nuclear Ribosomal Dna, Donald Padgett, D. H. Les, G. E. Crow Jan 1999

Phylogenetic Relationships In Nuphar (Nymphaeaceae): Evidence From Morphology, Chloroplast Dna, And Nuclear Ribosomal Dna, Donald Padgett, D. H. Les, G. E. Crow

Biological Sciences Faculty Publications

The genus Nuphar consists of yellow-flowered waterlilies and is widely distributed in north-temperate bodies of water. Despite regular taxonomic evaluation of these plants, no explicit phylogenetic hypotheses have been proposed for the genus. We investigated phylogenetic relationships in Nuphar using morphology and sequences of the chloroplast gene matK and of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA. Two major lineages within Nuphar are consistently resolved with the morphological and molecular data sets. One lineage comprises New World taxa and the other represents a primarily Old World lineage. Relationships within the major lineages were poorly resolved by morphology …


Reproductive Biology And Search Behavior Of Amitus Bennetti (Hymenoptera: Platygasteridae), A Parasitoid Of Bemisia Argentifolii (Homoptera: Aleyrodidae), Andrea L. Joyce, Thomas S. Bellows, Jr., David H. Headrick Jan 1999

Reproductive Biology And Search Behavior Of Amitus Bennetti (Hymenoptera: Platygasteridae), A Parasitoid Of Bemisia Argentifolii (Homoptera: Aleyrodidae), Andrea L. Joyce, Thomas S. Bellows, Jr., David H. Headrick

Horticulture and Crop Science

No abstract provided.


Potential Associations Among Genetic Markers In The Serotonergic System And The Antisocial Alcoholism Subtype, E. M. Hill, S. F. Stoltenberg, M. Burmeister, M. Closser, R. A. Zucker Jan 1999

Potential Associations Among Genetic Markers In The Serotonergic System And The Antisocial Alcoholism Subtype, E. M. Hill, S. F. Stoltenberg, M. Burmeister, M. Closser, R. A. Zucker

Department of Psychology: Faculty Publications

Alcoholism is transmitted in families. The complexity and heterogeneity of this disorder has made it difficult to identify specific genetic correlates. One design with the potential to do so is the family-based association study, in which the frequencies of genetic polymorphisms are compared between affected and nonaffected members. Reduced central serotonin neurotransmission is associated with features of an antisocial subtype of alcoholism, although a primary deficit has not been traced to a particular component. Genetic markers related to the serotonergic system have been identified, located, and cloned. If associations can be discovered, the development process for pharmacotherapy could be facilitated. …


Fmri Of Monkey Visual Cortex, Lisa Stefanacci, Paul Reber, Jennifer Costanza, Eric Wong, Richard Buxton, Stuart Zola, Larry R. Squire, Thomas D. Albright Jun 1998

Fmri Of Monkey Visual Cortex, Lisa Stefanacci, Paul Reber, Jennifer Costanza, Eric Wong, Richard Buxton, Stuart Zola, Larry R. Squire, Thomas D. Albright

Neurology Collection

While functional magnetic resonance imaging (fMRI) is now used widely for demonstrating neural activity-related signals associated with perceptual, motor, and cognitive processes in humans, to date this technique has not been developed for use with nonhuman primates. fMRI in monkeys offers a potentially valuable experimental approach for investigating brain function, which will complement and aid existing techniques such as electrophysiology and the behavioral analysis of the effects of brain lesions. There are, however, a number of significant technical challenges involved in using fMRI with monkeys. Here, we describe the procedures by which we have overcome these challenges to …


Factors Concerning The Conservation Of A Rare Shale Endemic Plant: The Reproductive Ecology And Edaphic Characteristics Of Penstemon Debilis (Scrophulariaceae), Amy L. Mcmullen May 1998

Factors Concerning The Conservation Of A Rare Shale Endemic Plant: The Reproductive Ecology And Edaphic Characteristics Of Penstemon Debilis (Scrophulariaceae), Amy L. Mcmullen

Biology

The management of a rare, edaphic endemic plant species requires an understanding of the ecological setting and processes that contribute to its restriction. Included in the ecological processes is a set of biotic characters associated with the reproductive ecology, including its breeding system, pollination vectors, and germination requirements. The geologic and soil setting of the plant must also be characterized in order to understand the plant's restriction to the substrate. These issues were addressed by this study of the rare endemic Penstemon debilis O'Kane and Anderson (Scrophulariaceae). Penstemon debilis consists of six populations that occur on talus slopes of the …


Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter May 1998

Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter

Nebraska Center for Virology: Faculty Publications

Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis -acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease.


The Effects Of Tactile/Kinesthetic Instructional Strategies In The Biology Classroom, Janice Batten Sees Apr 1998

The Effects Of Tactile/Kinesthetic Instructional Strategies In The Biology Classroom, Janice Batten Sees

Theses and Dissertations

The purpose of this study was to determine whether the addition of tactile/kinesthetic instruction to the biology classroom could significantly increase learning. Various kinds of tactile/kinesthetic lessons were taught to three experimental groups who also received visual and auditory instruction. These tactile/kinesthetic lessons included task cards, task puzzles, manipulatives, total body movement, and large floor games. The control group only received visual and auditory instruction. The subjects were 84 College Biology students from Buena Regional High School, a school in rural South Jersey. The classes varied in gender and race. The same instructor taught all classes and the length of …


Developmental And Hormonal Regulation Of The Expression Of Cytosolic Phospholipase A2 Protein Levels In Baboon Placenta, Kathleen Elizabeth Dunstan Apr 1998

Developmental And Hormonal Regulation Of The Expression Of Cytosolic Phospholipase A2 Protein Levels In Baboon Placenta, Kathleen Elizabeth Dunstan

Chemistry & Biochemistry Theses & Dissertations

Cytosolic phospholipase A2 ( cPLA2) specifically catalyzes the release of arachidonate from membrane phospholipids, thus initiating synthesis of prostaglandins, thromboxanes, and other eicosanoids. The human and nonhuman primate placenta actively synthesize high levels of eicosanoids which play both an autocrine and paracrine role in placental function. Previous studies in Dr. Miriam D. Rosenthal's laboratory have shown that cPLA2 mRNA levels are developmentally regulated. The present study was designed to determine whether placental cPLA2 protein levels increased during gestation. Since the marked increases in estrogen levels during normal pregnancy have been shown to enhance morphological and …


Use Of Baculovirus As Expression Vector In Biotechnology, Zihni Demi̇rbağ, A. Osman Beldüz, İsmail Demi̇r Jan 1998

Use Of Baculovirus As Expression Vector In Biotechnology, Zihni Demi̇rbağ, A. Osman Beldüz, İsmail Demi̇r

Turkish Journal of Biology

Variuos viral, bacterial, plant and animal proteins which are important for medicinal, industrial and agricultural aspects, have been synhesized via expression vector systems have great importance in biotechnology. Baculoviruses, used as a good model system in molecular biology studies, a biological control agent in agriculture and an expression vector for synthesis of important proteins, opened up a significant period for recombinant DNA technology and protein production. Desired foreign genes, code for various products such as drug, toxin and nutrient substances, can be replaced with viral genes which are not essasial for replication of baculovirus in cell culture. Priority and advantages …


Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna Jan 1998

Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult pig liver cells. Adult pig hepatocytes exhibited limited or no replication and were lost from the secondary culture at Passage 3 or 4. In contrast, adult pig bile duct cells replicated and were carried for 4-8 passages in secondary culture. A simple method to produce nearly pure pig intrahepatic bile duct cultures was first to freeze a relatively crude liver cell preparation. Upon subsequent thawing, all hepatocytes and most macrophages …


Mummies And Mummification Practices In The Southern And Southwestern United States, Mahmoud Y. El-Najjar, Thomas M. J. Mulinski, Karl Reinhard Jan 1998

Mummies And Mummification Practices In The Southern And Southwestern United States, Mahmoud Y. El-Najjar, Thomas M. J. Mulinski, Karl Reinhard

Karl Reinhard Publications

Mummification was not intentional for most North American prehistoric cultures. Natural mummification occurred in the dry areas of North America, where mummies have been recovered from rock shelters, caves, and overhangs. In these places, corpses desiccated and spontaneously mummified. In North America, mummies are recovered from four main regions: the southern and southwestern United States, the Aleutian Islands, and the Ozark Mountains of Arkansas. This chapter is limited to a discussion of burial practices and a tabulation of the location of mummies in the southwestern United States with some comments on mummies of the southern United States, including the Ozarks.


Limited Range Of Genetic Variation In Echinococcus Multilocularis, Heinz Rinder, Robert L. Rausch, Kenichi Takahashi, Hartmuth Kopp, Angelika Thomschke, Thomas Löscher Dec 1997

Limited Range Of Genetic Variation In Echinococcus Multilocularis, Heinz Rinder, Robert L. Rausch, Kenichi Takahashi, Hartmuth Kopp, Angelika Thomschke, Thomas Löscher

Harold W. Manter Laboratory of Parasitology: Faculty Publications

DNA sequencing of 1.3 kb of rDNA containing both internal transcribed spacers (ITS1, ITS2) and adjoining rRNA coding regions in each of 11 Echinococcus multilocularis isolates from Germany, Japan, and Alaska resulted in identical nucleotide sequences except for a single polymorphic locus 54 bp upstream of the 3' end of the 18S coding region, separating Eurasian isolates from an Alaskan isolate. The same base substitution was found in each of two additional isolates from Alaska. The distribution of the resulting genotypes with regard to their origin is highly significant (>99.9%) and corresponds to the traditional subspecies Echinococcus multilocularis multilocularis …


The Effects Of Temperature Regime And Food Availability On Growth Rates Of Young-Of-The-Year Colorado Squawfish (Ptychocheilus Lucius) In The Green River, Utah, Craig J. Schaugaard May 1997

The Effects Of Temperature Regime And Food Availability On Growth Rates Of Young-Of-The-Year Colorado Squawfish (Ptychocheilus Lucius) In The Green River, Utah, Craig J. Schaugaard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of different daily temperature regimes and food availability on growth rates of Colorado squawfish (Ptychocheilus lucius) were determined using field and laboratory experiments. Daily temperature and food gradients were observed in river backwaters (important nursery habitat for juvenile Colorado squawfish). High fluctuating temperatures (± 5-8° C) were observed daily in the shallow, terminal ends of backwaters. Where backwaters were in contact with the river (mouth), daily temperature fluctuations decreased (± 2° C). Food availability also varied spatially between the mouth and end of each backwater. To test whether Colorado squawfish growth rates varied in different areas …


Nerve Tissue-Specific Human Glutamate Dehydrogenase That Is Thermolabile And Highly Regulated By Adp / P. Shashidharan, Donald D. Clarke, Naveed Ahmed, Nicholas Moschonas, And Andreas Plaitakis Department Of Neurology, Mount Sinai School Of Medicine, New York; Department Of Chemistry, Fordham University, Bronx New York, Usa; And Department Of Biology And School Of Health Sciences, University Of Crete, Crete, Greece, P. Shashidharan, Donald Dudley Clarke Phd, Naveed Ahmed, Nicholas Moschonas Jan 1997

Nerve Tissue-Specific Human Glutamate Dehydrogenase That Is Thermolabile And Highly Regulated By Adp / P. Shashidharan, Donald D. Clarke, Naveed Ahmed, Nicholas Moschonas, And Andreas Plaitakis Department Of Neurology, Mount Sinai School Of Medicine, New York; Department Of Chemistry, Fordham University, Bronx New York, Usa; And Department Of Biology And School Of Health Sciences, University Of Crete, Crete, Greece, P. Shashidharan, Donald Dudley Clarke Phd, Naveed Ahmed, Nicholas Moschonas

Chemistry Faculty Publications

Glutamate dehydrogenase (GDH), an enzyme that is central to the metabolism of glutamate, is present at high levels in the mammalian brain. Studies on human leukocytes and rat brain suggested the presence of two GDH activities differing in thermal stability and allosteric regulation, but molecular biological investigations led to the cloning of two human GDH-specific genes encoding highly homologous polypeptides. The first gene, designated GLUD1, is expressed in all tissues (housekeeping GDH), whereas the second gene, designated GLUD2, is expressed specifically in neural and testicular tissues. In this study, we obtained both GDH isoenzymes in pure form by expressing a …


Roles Of Phospholipase D And Sphingomyelinase In T-Cell Signal Transduction, Cynthia Denise Bradshaw Jan 1997

Roles Of Phospholipase D And Sphingomyelinase In T-Cell Signal Transduction, Cynthia Denise Bradshaw

MUSC Theses and Dissertations

The overall goal of this study was to investigate the roles of PLD and neutral SMase in signal transduction in T-lymphocytes. In particular, the abilities of phospholipid metabolites to regulate mitogen-activated protein kinases were explored. The tumor promoting PKC activator, phorbol 12-myristate 13-acetate (PMA), and an antileukemic drug, 1-β-D arabinofuranosylcytosine hydrochloride (Ara-C), were used as model phospholipase activators. The effects of phorbol ester and Ara-C on PLD and SMase activities were examined in EL4, a murine thymoma cell line, and Jurkat, a human leukemia cell line. PLD activity was measured in intact cells using a metabolic labelling assay. PLD and …


Analysis Of The Inheritance Repression Of A Transposable Element In Drosophila Melanogaster, Melissa Leone Jan 1997

Analysis Of The Inheritance Repression Of A Transposable Element In Drosophila Melanogaster, Melissa Leone

Master's Theses

No abstract provided.


Characterization Of Adaptive Changes In 5-Htia Receptor Systems Induced By Repeated Injections Of 5-Ht Uptake Inhibitors, Qian Li Jan 1997

Characterization Of Adaptive Changes In 5-Htia Receptor Systems Induced By Repeated Injections Of 5-Ht Uptake Inhibitors, Qian Li

Dissertations

No abstract provided.


Flea News 53 (December 1996), Robert E. Lewis Dec 1996

Flea News 53 (December 1996), Robert E. Lewis

Flea News

Robert E. Lewis to retitre from Iowa State University effective January 1, 1997

Book reviews

Obituaries: Robert S. Traub (October 26, 1916-December 21, 1996); Dorald Mervin Allred (July 11, 1923-June 20, 1996)

Miscellanea

Siphonaptera literature

Report: Use of an orally administered insect development inhibitor (Lufenuron) as a flea control agent in the California ground squirrel, Spermophilus beecheyi by Richard M. Davis


Ard News December 1996 Dec 1996

Ard News December 1996

Agricultural Research Division: News and Annual Reports

CONTENTS:

SEASON'S GREETINGS
REVIEW PROCEDURES FOR NEW AND REVISED REGIONAL RESEARCH PROJECTS AND COMMITTEES
CROP DIAGNOSTIC CLINIC WELL-RECEIVED BY AGRIBUSINESS PROFESSIONALS
COMMUNITY GETS INVOLVED IN MANURE MANAGEMENT RESEARCH AT THE ARDC
RECOGNITION OF JUNIOR FACULTY FOR EXCELLENCE IN RESEARCH
LAYMAN AWARDS
NEW OR REVISED PROJECTS
INNOVATIVE AND HIGH RISK RESEARCH PROGRAM
PROPOSALS SUBMITTED FOR FEDERAL GRANTS
GRANTS AND CONTRACTS RECEIVED OCTOBER AND NOVEMBER, 1996


A Scanning Electron Microscopy Study Of Taxus Leaves As Related To Taxonomy, G.A. Strobel, W. M. Hess Nov 1996

A Scanning Electron Microscopy Study Of Taxus Leaves As Related To Taxonomy, G.A. Strobel, W. M. Hess

Scanning Microscopy

Scanning electron microscopy, when applied to the surfaces of the needles of Taxus spp. (yew) revealed features that appear useful in the taxonomy of this tree species which yields the important anticancer drug, taxol. For instance, all of the four North American species have 3-5 rows of stomata on one-half of the abaxial leaf surface, whereas all of the others, including those from Europe and Asia, have 7-10 rows of stomata. The appearance of individual or fused papilliform epidermal cells and their arrangement on the leaf surface also is a feature that varies between species. Patterns of wax formation appeared …


Ard News October 1996 Oct 1996

Ard News October 1996

Agricultural Research Division: News and Annual Reports

Contents:

Comments from the Associate Director
Fund for Rural America
FY 1997 CSREES Budget
Mussehl Endowment
ESCOP/ACOP Leadership Development Program
Proposals Submitted for Federal Grants
Nebraska Soybean Development, Utilization and Marketing Board
New or Revised Projects


Pre-Embedding Staining Of Single Muscle Fibers For Light And Electron Microscopy Studies Of Subcellular Organization, Evelyn Ralston, Thorkil Ploug Jul 1996

Pre-Embedding Staining Of Single Muscle Fibers For Light And Electron Microscopy Studies Of Subcellular Organization, Evelyn Ralston, Thorkil Ploug

Scanning Microscopy

Skeletal muscle fibers are large, multinucleated cells which pose a challenge to the morphologist. In the course of studies of the distribution of the glucose transporter GLUT4, in muscle, we have compared different preparative procedures, for both light (LM) and electron microscopy (EM) immunocytochemistry. Here we show that pre-embedding staining of single teased fibers, or of single enzymatically dissociated fibers, has several advantages over the use of sections for observing discrete patterns that extend over long distances in the cells. We report on an optimization study carried out to establish fixation and permeabilization conditions for EM immunogold labeling of the …