Digital Commons Network^™

Exosense: A Novel Technology For Selective Purification Of Cd63+ Exosomes From Neural Cells, Kristen Hope Hutson Truelove

Master's Theses

Exosomes are extracellular, cell signaling microvesicles that contain unique genomic and proteomic signatures reflective of the host cell’s pathophysiological conditions. In recent years, the study of exosomes has increased tremendously because many have been recognized as molecular biomarkers with the potential to advance methods of disease diagnostics and therapeutics as well as contribute to physiological analyses of multiple organism types. With the promising potential that exosomes offer to the field of molecular biology, it is vital to establish an efficient and consistently reliable mechanism of exosome isolation from biological samples. Many isolation techniques currently available typically yield exosome samples with …

Wi-Fi-Based Indoor Localization Using Model-Based And Data-Driven Approaches, Ayoub Idelhaj

Electrical Engineering Theses - Archive

This thesis investigates model-based and data-driven approaches for indoor localization using the Received Signal Strength Indicator (RSSI) of Wi-Fi signals. We study multiple model-based indoor localization approaches, including the free space path loss model, the log-distance path loss model, the International Telecommunication Union (ITU) model, and a nonlinear regression model. We examine their indoor localization accuracy using raw RSSI values, and filter RSSI values passed through a Moving Average filter and a Kalman filter. For data driven approaches, we employ a family of Extreme Learning Machine (ELM) algorithms including Basic-ELM, Online Sequential-ELM (OS-ELM), Hierarchical-ELM (H-ELM), and Kernel-ELM (K-ELM), to find …

Recent Studies On The Synthesis Of Medicinal Molecules., Paige Monsen

Electronic Theses and Dissertations

Medicinal chemistry interfaces synthetic organic chemistry, natural product chemistry and chemical biology, with a goal of yielding therapeutic agents. Natural products or compounds derived from natural sources such as plants, animals, and microorganisms, are often biologically active and render that compound a likely drug lead. For thousands of years humankind has utilized natural products for medicinal purposes and consequently scientists take advantage of both these compounds’ core structural characteristics and their modes of actions on selected targets as inspiration to develop therapeutics. Because the total synthesis of such complex molecules can be cumbersome and expensive, semi-synthetic methods on isolated natural …

Dissecting The Mechanisms Of Venetoclax Resistance In Myelodysplastic Syndromes, Shuaitong Chen

Dissertations and Theses (Open Access)

Myelodysplastic syndromes (MDS) are a class of heterogeneous clonal hematopoietic disorders. The current standard of care for MDS is the hypomethylating agent (HMA)-based therapy. However, only 50 percent of the patients respond, with transient effects and no approved second-line treatment options after their diseases progress to acute myeloid leukemia (AML). The mechanisms that govern HMA failure are unknown. Recently, we unveiled that MDS are maintained and propagated by two different immunophenotypically distinct hierarchical cellular organizations of MDS hematopoietic stem and progenitor cells (HSPCs) through the upregulation of specific survival pathways. This finding allowed us to potentially stratify MDS patients into …

A Tale Of Itam Tails: New Insights Into The Molecular Mechanisms That Tune Fcɛri Signaling, William K. Kanagy

Biomedical Sciences ETDs

Immunoreceptor Tyrosine-based Activating Motif (ITAM) receptors are critical in sensing extracellular antigen and initiating an immune response. The ITAM receptor FcɛRI, termed the high affinity IgE receptor, is the primary promoter of inducing the Type 1 allergic response and is primarily expressed in mast cells and basophils. In this dissertation, I worked to determine how early signaling events initiated at the FcɛRI ITAM interface tune downstream signaling outcomes. I began by determining that differential phospho-tyrosine ITAM patterns produce unique recruitment profiles of the accessory kinases proteins Syk and Lyn. I next determined the differential signaling ITAM capabilities of Lyn and …

Exploring The Functions Of Phf20/Phf20l1 Within The Nsl-Transcriptional Complex, Daniela Ruiz, Peter R. Harkins

Summer Experience 2021

No abstract provided.

Threshold Concepts In Web Development: The Impact Of Education And Experience On The Perceptions Of Practitioners, Michael Lee Mick

College of Computing and Digital Media Dissertations

The Threshold Concepts (TCs) framework posits characteristics of critical concepts that enable a learner to see things in a new and unforgettable way that allows the incorporation of cognitive approaches and skills needed by professionals in the discipline. All previous research has been qualitatively oriented toward discovery of candidate TCs, without, so far, quantitative studies of the candidate TCs recognition within the professional ranks. An underrepresented group in previous research is that of Web development professionals.

This proposal describes initial quantitative research utilizing the TCs framework in the Web development area to measure the perception of candidate TCs by professionals …

Causal Mediation Analysis With Multiple Time-Varying Mediators, An-Shun Tai, Sheng-Hsuan Lin, Yu-Cheng Chu, Tsung Yu, Milo A. Puhan, Tyler Vanderweele

Harvard University Biostatistics Working Paper Series

In longitudinal studies with time-varying exposures and mediators, the mediational g-formula is an important method for the assessment of direct and indirect effects. However, current methodologies based on the mediational g-formula can deal with only one mediator. This limitation makes these methodologies inapplicable to many scenarios. Hence, we develop a novel methodology by extending the mediational g-formula to cover cases with multiple time-varying mediators. We formulate two variants of our approach that are each suited to a distinct set of assumptions and effect definitions and present nonparametric identification results of each variant. We further show how complex causal mechanisms (whose …

Thanatechnology And The Living Dead: New Concepts In Digital Transformation And Human-Computer Interaction, Vural Özdemir, Simon Springer, Arif Ylldlrlm, Sehmus Biçer, Asllgül Kendirci, Semra Sardaş, Hakan Klllç, Nezih Hekim, Tanja Kunej, Kazlm Yalçln Arga, Kevin Dzobo, Wei Wang, Marius Geanta, Angela Brand, Mustafa Bayram

Research outputs 2014 to 2021

In a digital society, shall we be the authors of our own experience, not only during our lifetime but also after we die? We ask this question because dying and bereavement have become even harder, and much less private, in the digital age. New big data-driven digital industries and technologies are on the rise, with promises of interactive 3D avatars and storage of digital memories of the deceased, so they can continue to exist online as the "living dead"in a digital afterlife. Famous rock and roll icons like Roy Orbison, Frank Zappa, Ronnie James Dio, and Amy Winehouse have famously …

Systematics Of Eastern North American Podothrombium (Parasitengona: Podothrombiidae), Kelsey Lynn Cline

Graduate Theses and Dissertations

Velvet mites of the genus Podothrombium Berlese, 1910 are found worldwide with only two described species known from North America, both occurring on the west coast. The present study describes five new species occurring in the east, from both larval and post-larval stages. Species hypotheses are supported with morphology and analysis of mitochondrial (barcoding region of COI) and nuclear genes (D2-3 expansion regions of 28S rDNA). Specimens are incorporated from the Barcode of Life Data Systems (BOLD), allowing our dataset to span most of North America. Therefore, we were able to raise the number of Podothrombium in North America from …

The Impact Of Using An Online Learning Management System On Student Biology Achievement In A Hispanic Rural High School, Brent J. Burkott

Theses and Dissertations

The purpose for this study was to determine the effect of using online Learning Management Systems with a Blended Learning pedagogy on state assessment achievement of high school biology students with interactions analyzed based on teaching method (Face-to-Face/Blended Learning), course type (Honors/Regular) biological sex (Male/Female), risk factor (At-Risk/Not At-Risk), and socioeconomic status (High/Low). The research questions were: (1) What is the difference in achievement scores between students taught in a Blended Learning environment with a Learning Management System verses a traditional Face-to-Face learning environment, and (2) What differences in achievement exist on the Texas assessment based on teaching method, course …

Comparative Transcriptome Analysis Reveals Genetic Mechanisms Of Sugarcane Aphid Resistance In Grain Sorghum, Desalegn D. Serba, Xiaoxi Meng, James Schnable, Elfadil Bashir, J P. Michaud, P V. Vara Prasad, Ramasamy Perumal

Department of Agronomy and Horticulture: Faculty Publications

The sugarcane aphid, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae) (SCA), has become a major pest of grain sorghum since its appearance in the USA. Several grain sorghum parental lines are moderately resistant to the SCA. However, the molecular and genetic mechanisms underlying this resistance are poorly understood, which has constrained breeding for improved resistance. RNA-Seq was used to conduct transcriptomics analysis on a moderately resistant genotype (TAM428) and a susceptible genotype (Tx2737) to elucidate the molecular mechanisms underlying resistance. Differential expression analysis revealed differences in transcriptomic profile between the two genotypes at multiple time points after infestation by SCA. Six gene …

Spatial Ecology And Stable Isotope Composition Of Cetaceans In The Bohol Sea, Edna Sabater

Theses and Dissertations

Studies on Philippine cetaceans (i.e., whales and dolphins) began in the early 1980s, and have resulted in the location and identification of species in Philippine waters. However, the population estimates of most of the cetacean species are uncertain, which is important in evaluating whether the populations are stable, decreasing, or increasing in relation to potential threats and conservation measures. I conducted line-transect surveys between 2010 and 2019 in the Bohol Sea, Philippines. A total of 2009.5 km survey efforts were made, which covered an area of 8041 km2 throughout the 5-year survey period. Distance sampling methods estimated the total abundance …

Does Raising The Arms Modify Head Tremor Severity In Cervical Dystonia?, Elizabeth Cisneros, Joel S Perlmutter, Et Al

Open Access Publications

Background: A defining characteristic of dystonia is its position-dependence. In cervical dystonia (CD), sensory tricks ameliorate head tremor (HT). But it remains unknown whether raising the arms alone has the same impact.

Methods: We analyzed data collected from patients enrolled by the Dystonia Coalition. For 120 patients with HT, we assessed how raising their arms without touching their head changed their HT severity.

Results: Forty-eight out of 120 patients exhibited changes in HT severity when raising their arms. These patients were more likely to exhibit decreases in HT severity (N = 35) than increases (N = 13, χ

Discussion: Raising …

Postojna-Planina Cave System In Slovenia, A Hotspot Of Subterranean Biodiversity And A Cradle Of Speleobiology, Maja Zagmajster, Slavko Polak, Cene Fišer

KIP Articles

The Postojna-Planina Cave System (PPCS) in central Slovenia is a globally exceptional site of subterranean biodiversity, comprised of many interconnected caves with cumulative passage length exceeding 34 km. Two rivers sink into the caves of the PPCS, called the Pivka and Rak, and join underground into Unica River, which emerges to the surface. The studies of fauna of PPCS began in the 19th century with the first scientific descriptions of specialized cave animals in the world, making it “the cradle of speleobiology”. Currently, the species list of PPCS contains 116 troglobiotic animal species belonging to eight phyla, confirming its status …

The Impact Of Global Crises On Women: The Case Of Covid-19 In Egypt, Nour A. Dokhan

Theses and Dissertations

Any global crisis is expected to affect every human being, but for women it is always twice as hard. In the case of Covid-19, women are more affected across every domain, from social protection to health, simply because if their gender. This research explores the impact of Covid-19 on women, both economically and socially, with the focus on the case of Egypt. It explores the systematic gender inequalities in the economic, social, and health spheres using cases from previous crises, and how this gender inequality and vulnerability has resulted in much worse consequences of Covid-19 than that of men. The …

Contemporary Challenges And Opportunities For The Management Of Bird Damage At Field Crop Establishment, Christophe Sausse, Alice Baux, Michel Bertrand, Elsa Bonnaud, Sonia Canavelli, Alexandra Destrez, Page E. Klug, Lourdes Olivera, Ethel Rodriguez, Guilllermo Tellechea, Sebastian Zuil

United States Department of Agriculture Wildlife Services: Staff Publications

Bird damage, from sowing to crop establishment, is an important issue for farmers in many parts of the world. However, reliable and cost-effective solutions remain elusive because management tools and research on the subject are limited. The spatial variability of damage across landscapes and the adaptative behaviour of birds create further challenges. Additionally, the issue must be tackled at the landscape scale and involve a variety of stakeholders with conflicting interests and objectives. We summarize some of the challenges and opportunities identified to face these difficulties and address four major research directions for operational solutions including 1) crop damage assessment, …

Biological Control And Integrated Rangeland Weed Management, Linda M. Wilson

IGC Proceedings (1977-2023)

No abstract provided.

Population Status Of Tapanuli Orangutan (Pongo Tapanuliensis) Within The Renewable Energy Development And Its Management Implications, Didik Prasetyo, Yokyok Hadiprakarsa, Wanda Kuswanda, Jito Sugardjito

Forest and Society

To protect Tapanuli orangutan it is essential to understand the actual situation. It has been studied 15% of its population live outside the protected area facing a density disruption due to forest conversion. Several best management practices have been created and tested for different natural concession types. Yet, the main objective to reduce the impact and increase wildlife survival is far away from the goal. To improve our understanding of the species survival within ongoing project construction, we conducted population density monitoring prior- to post-construction time frames within the hydroelectric dam project. Also, we carried out spatial analysis to understand …

Evaluation Of Somatic Mutations In Solid Metastatic Pan-Cancer Patients, Moom Roosan, Isa Mambetsariev, Rebecca Pharaon, Jeremy Fricke, Angel R. Baroz, Joseph Chao, Chen Chen, Mohd W. Nasser, Ramakanth Chirravuri-Venkata, Maneesh Jain, Lynette Smith, Susan E. Yost, Karen L. Reckamp, Raju Pillai, Leonidas Arvanitis, Michelle Afkhami, Edward W. Wang, Vincent Chung, Mihaela Cristea, Marwan Fakih, Marianna Koczywas, Erminia Massarelli, Joanne Mortimer, Yuan Yuan, Surinder K. Batra, Sumanta Pal, Ravi Salgia

Pharmacy Faculty Articles and Research

Metastasis continues to be the primary cause of all cancer-related deaths despite the recent advancements in cancer treatments. To evaluate the role of mutations in overall survival (OS) and treatment outcomes, we analyzed 957 metastatic patients with seven major cancer types who had available molecular testing results with a FoundationOne CDx^® panel. The most prevalent genes with somatic mutations were TP53, KRAS, APC, and LRP1B. In this analysis, these genes had mutation frequencies higher than in publicly available datasets. We identified that the somatic mutations were seven mutually exclusive gene pairs and an additional fifty-two co-occurring gene pairs. Mutations …

Development Of A Split Green Fluorescent Protein Reporting System For Angiogenin, Phan QuỳNh ChâU Lê

Lawrence University Honors Projects

Angiogenin (ANG) is a secreted protein that holds important implications in the growth and survival for a variety of cell types—from healthy endothelial or neuronal cells, to diseased cancer cells. Currently, we lack a complete understanding of ANG’s mechanism of action. Our work aims to develop a functional fluorescence-based reporting system to study ANG internalization activity in cancer cells. Specifically, we utilize a split green fluorescent protein (split GFP) system, in which the 11th strand of the GFP molecule (GFP11) is attached to ANG, while the remaining non-fluorescent GFP1-10 fragment is expressed in our target HeLaGFP1-10 cell line. The reconstitution …

Walking Velocity And Estimated Distance Of The Armored Scale Crawler Rhizaspidiotus Donacis, A Biological Control Agent For Arundo Donax, Joshua A. Villarreal, Alexis Racelis, John A. Goolsby

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Arundo scale, Rhizaspidiotus donacis (Leonardi) (Hemiptera: Diaspididae), is an armored scale biological control agent established on the invasive weed, Arundo donax L. (Poaceae; Arundinoideae) at several locations along the Rio Grande in Texas (Goolsby et. al. 2009, Moran and Goolsby 2010, Goolsby et al. 2011, Villarreal et al. 2016). The arundo scale is having significant impact on the target weed in areas where it established (Goolsby and Moran 2019), and no non-target plant use was observed (Goolsby et al. 2020). Armored scale crawlers usually live for less than a day and …

Mechanistic And Synthetic Studies Of Oxidopyrylium Cycloaddition Reactions, Lauren P. Bejcek

Dissertations, Theses, and Capstone Projects

Cycloaddition chemistry has historically served as a robust route to stereo-rich and highly functionalized cyclic and heterocyclic compounds. Herein we describe our examination of 3-hydroxy-4-pyrone-derived oxidopyrylium cycloadditions in both mechanistic considerations as well as the unique synthetic advancements of their corresponding cycloadducts. Chapter 1 is a literature review where we specifically outline the use of oxidopyrylium cycloadditions in the synthesis of natural products. This includes the mention of previous syntheses prior to 2008, and a more thorough examination of the natural products synthesized from 2008 to present. The work exploits this chemistry as a facile route to highly complex 7-membered …

Flaxseed’S Paradoxical Role In Extending Lifespan And Reproductive Capacity In White Leghorn Laying Hens; And The Effect Of Polyunsaturated Fatty Acids (Pufas) On Lipid Metabolism, Mitochondrial Bioenergetics And E-Cadherin Expression In Laying Hen Ovarian Tumors, William Christopher Weston

Dissertations

We are the first lab to report the occurrence of a diet-induced transsulfuration (TS) blockade associating with elevated S-adenosylmethionine (i.e. SAM) synthesis, enhanced lifespan and enhanced reproductive capacity, in a vertebrate animal model. In this paradoxical study, we used LC-MS/MS-derived metabolomics data to report the effects of flaxseed (Linum Usitatissimum) on one-carbon metabolism in White Leghorn laying hens (Gallus gallus). Flaxseed contains a vitamin B6-antagonizing molecule called ‘linatine’ that is particularly effective at reducing vitamin B6 levels in small rodents and poultry. Linatine reduces TS flux through its inhibition of the vitamin B6-dependent enzymes cystathionine beta synthase (CBS) and cystathionine …

Synthetic And Biological Studies On Benzazepine Derivatives As Dopamine Receptor Ligands, Rajan Giri

Dissertations, Theses, and Capstone Projects

Dopamine (DA) receptors, members of the G-protein coupled receptors (GPCRs) family, are divided in two groups based on their transmembrane structural homology domains: D1R-like (D1R, D5R sub-types) and D2R-like DA receptors (D2R, D3R and D4R sub-types). Disturbances in dopaminergic neurotransmission are associated with several CNS disorders. Hence, DA receptor selective ligands have been sought as pharmacological agents to normalize perturbations in the dopaminergic system. Despite several notable efforts, the discovery of highly selective ligands for dopamine receptor sub-types has proved challenging due to close transmembrane structural similarity, especially between DA receptor sub-types within the same group.

The 1-phenylbenzazepine scaffold is …

Controlled Reduction Of Graphene Oxide And Graphene Oxide-Carbon Nanotube Hybrids And Their Applications, Samar Azizighannad

Dissertations

Graphene and graphene derivatives are widely used in diverse research and industrial applications. Graphene production on a large scale is carried out by exfoliating graphite oxide and producing graphene oxide (GO), which comprises of graphene sheets with different oxygen-containing functional groups such as hydroxyl, carboxyl, and carbonyl. GO is reduced to reduced graphene oxide (rGO), which has properties that are closer to graphene. Properties of GO and rGO depend upon the oxygen content, and the effect of reducing oxygen content on the aqueous behavior of rGOs is not well understood.

In an effort to understand how properties of rGO change …

Modeling And Analysis Of Intracellular Signaling Networks And Cellular Decisions, Mustafa Ozen

Dissertations

Developing molecular network analysis methods is important due to their applications on complex biological systems such as target discovery, development of drugs, discovering drug effects, and finding treatments for many complex diseases, e.g., cancer, autoimmune, and mental disorders. An example of analysis techniques is the fault diagnosis analysis, in which the purpose is to quantify how much vulnerable the entire network is to dysfunction of one or multiple molecules. Such analysis can be done after proper network models are implemented, trained, and tested against the experimental data. In this dissertation, a Boolean modeling framework is implemented and methods to train …

Lampenflora In A Show Cave In The Great Basin Is Distinct From Communities On Naturally Lit Rock Surfaces In Nearby Wild Caves, Jake Burgoyne, Robin Crepeau, Jacob Jensen, Hayden Smith, Gretchen Baker, Steven D. Leavitt

KIP Articles

In show caves, artificial lighting is intended to illuminate striking cave formations for visitors. However, artificial lighting also promotes the growth of novel and diverse biofilm communities, termed lampenflora, that obtain their energy from these artificial light sources. Lampenflora, which generally consist of cyanobacteria, algae, diatoms, and bryophytes, discolor formations and introduce novel ecological interactions in cave ecosystems. The source of lampenflora community members and patterns of diversity have generally been understudied mainly due to technological limitations. In this study, we investigate whether members of lampenflora communities in an iconic show cave—Lehman Caves—in Great Basin National Park (GRBA) in the …

Homosexuality And Adoption Of Children: A Bibliometric Analysis, Karthiayani A. Ms., Manika Kamthan Dr.

Library Philosophy and Practice (e-journal)

This study is based on the bibliometric analysis of research publications that focus on highlighting the impact of homosexuality on the process of adoption of children. The primary objective of this study is to analyze the frequency of publications focusing on the impact of parental sexual orientation on the process of adoption in different countries. The data required for this study was collected from the Scopus database and was analyzed using VOSviewer software. Literature published from 2000 to January 2021 were extracted and analyzed. A total of 284 documents which are classified into articles, letters, editorials, conference papers, and reviews …

Reu Site: Stem For Plant Health, David W. Britt

Funded Research Records

No abstract provided.