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The Status Of The Endangered Rafflesia And Conservation Efforts In Java Island, Indonesia, Bahana Aditya Adnan, Purnomo Purnomo, Suwarno Hadisusanto May 2026

The Status Of The Endangered Rafflesia And Conservation Efforts In Java Island, Indonesia, Bahana Aditya Adnan, Purnomo Purnomo, Suwarno Hadisusanto

Jurnal Penelitian Kehutanan Wallacea

Rafflesia species (Rafflesiaceae) is a small endo-holoparasitic South-East Asian plant genus known for its exceptionally large flowers, rare species, and high island endemism. Three species of Rafflesia, i.e. R. patma, R. rochussenii, and R. zollingeriana, are known from Java, Indonesia. All three species are threatened with extinction due to human activities that cause habitat loss and fragmentation. Our literature review revealed that currently, factors influencing the existence of this genus, including its life cycle and environmental factors that support its life, are still not well understood. In particular, studies of the distribution and population of Rafflesia on the …


Site Characterization And Integrated Irrigation-Forestry Solutions For Mangrove Restoration In The Red River Delta Biosphere Reserve, Vietnam, Manh Quy Do, Huy Quoc Nguyen, Minh Duc Nguyen, My Tra Ha, Tuan Anh Le, Luan Thanh Nguyen, Tu Ngoc Pham May 2026

Site Characterization And Integrated Irrigation-Forestry Solutions For Mangrove Restoration In The Red River Delta Biosphere Reserve, Vietnam, Manh Quy Do, Huy Quoc Nguyen, Minh Duc Nguyen, My Tra Ha, Tuan Anh Le, Luan Thanh Nguyen, Tu Ngoc Pham

Jurnal Penelitian Kehutanan Wallacea

The research was conducted in 2023 in a degraded mangrove forest area in Giao Hoa commune, Ninh Binh province, Vietnam. This area is part of the Red River Delta Biosphere Reserve (RRDBR), which was recognised by UNESCO in 2004. The study employed methods such as surveying topographical features, assessing soil and water characteristics, and evaluating the status of the mangrove forest. It also used GIS and The Analytic Hierarchy Process method to analyse and evaluate the current condition of the forest and its site characteristics. The research results show that, regarding the topographical features of the degraded mangrove area, it …


Urban Bird Community Interaction With Ficus And Non-Ficus Tree, Eka Alifikri, Yeni Aryati Mulyani, Ani Mardiastuti May 2026

Urban Bird Community Interaction With Ficus And Non-Ficus Tree, Eka Alifikri, Yeni Aryati Mulyani, Ani Mardiastuti

Jurnal Penelitian Kehutanan Wallacea

Ficus trees are recognised as keystone species in forest ecosystems and are crucial for urban wildlife, especially birds. IPB Darmaga Campus, a biodiversity-rich urban area, hosts a variety of plant species, including Ficus and non-Ficus tree species, along with diverse bird species. This study aimed to compare the utilisation of Ficus and non-Ficus plants by birds in the area. Observations were conducted on 5 Ficus benjamina, 5 Ficus microcarpa, and 10 non-Ficus tree individuals. Ficus trees were used by 15 bird species from 12 families, with 1700 recorded interactions. In contrast, non-Ficus trees were …


Cyanine Dyes As Next-Generation Fluorophores: Applications In Cell Sorting And Fingerprinting Technologies, Super-Multiplexed Spectroscopy, Biomedical Imaging, And Beyond, Lucas Allen Davis May 2026

Cyanine Dyes As Next-Generation Fluorophores: Applications In Cell Sorting And Fingerprinting Technologies, Super-Multiplexed Spectroscopy, Biomedical Imaging, And Beyond, Lucas Allen Davis

Undergraduate Honors Thesis Collection

As biochemical, pharmaceutical, and medical sciences advance, the demand for technologies capable of probing multiple cellular targets simultaneously continues to increase. Super-multiplexed spectroscopic techniques offer a pathway toward comprehensive cellular fingerprinting by overcoming the spectral limitations of conventional fluorescence labeling. By circumventing the traditional “color barrier,” cyanine-based Raman tags enable the simultaneous discrimination of numerous cellular morphologies, allowing spectroscopy and imaging to be integrated for high-dimensional cellular analysis. Technologies developed in Goda Lab—including high-throughput flow cytometry, virtual freezing fluorescence imaging (VIFFI), Fourier-transform coherent anti-Stokes Raman spectroscopy (FT-CARS), and MERdot nanoparticles—address longstanding trade-offs between throughput, resolution, sensitivity, and multiplexing capacity. Central …


Shark Immunology And Its Biomedical Potential: Evolution, Innovation, And The Imperative Of Conservation, Reesha Bhagat May 2026

Shark Immunology And Its Biomedical Potential: Evolution, Innovation, And The Imperative Of Conservation, Reesha Bhagat

Undergraduate Honors Thesis Collection

This review aims to shed light on a significant ecological deficit and its implications for the future of science and medicine. Sharks possess a uniquely adaptive, cancer-resistant, and evolutionarily refined immune system with transformative biomedical potential, making their conservation a scientific and medical imperative. We are losing something medically irreplaceable, and we don’t even fully understand it yet. Shark conservation is a pressing scientific imperative because sharks are ecologically essential and represent an irreplaceable biomedical resource.


Assessing The Conservation Needs Of Native Texas Crayfish Species Through Distribution Analysis And Species Distribution Modeling, Elizabeth J. Croft May 2026

Assessing The Conservation Needs Of Native Texas Crayfish Species Through Distribution Analysis And Species Distribution Modeling, Elizabeth J. Croft

Biology Theses

Crayfish have essential roles in ecosystems because they are ecosystem engineers and keystone species. However, crayfish species are threatened by degradation and loss of habitats, introduction of invasive species, and high levels of endemism. Texas provides no legal protections for crayfish, despite its large number of species and endemics in the state. The overall goal of this study is to clarify the distributions of native Texas crayfish species using literature records, citizens science, and field sampling data, and highlight species in need of more conservation research. Occurrence records from all collected data were combined to visualize the distributions of species …


The Effects Of Heat Stress On White Blood Cell Profiles And Antibody Concentrations In Broilers Genetically Selected For Low Water Conversion Ratio Compared To The Modern Random Bred Broiler, Luke B. Janski May 2026

The Effects Of Heat Stress On White Blood Cell Profiles And Antibody Concentrations In Broilers Genetically Selected For Low Water Conversion Ratio Compared To The Modern Random Bred Broiler, Luke B. Janski

Poultry Science Undergraduate Honors Theses

Heat stress (HS) is one of the most significant environmental stressors impacting poultry production worldwide, reducing growth, feed efficiency, welfare, and immune competence. With global temperatures rising, understanding physiological and immunological adaptations to HS is essential for sustainable production. Broiler chickens selectively bred for low water conversion ratio (LWCR) have shown potential for reduced water consumption under environmental challenges; however, their immune resilience under HS conditions compared with modern random-bred (MRB) broilers remains unclear. This study investigated the effects of cyclic HS on red- (RBC) and white- (WBC) blood cells and humoral immunity in LWCR and MRB broilers. Birds were …


A Series Of Investigations On The L. Cornutus Host System And Bivesiculid Sp. Cercariae, Briana K. Zaffiro May 2026

A Series Of Investigations On The L. Cornutus Host System And Bivesiculid Sp. Cercariae, Briana K. Zaffiro

All HCAS Student Capstones, Theses, and Dissertations

Rising global temperatures are likely modulating parasite-host interactions, yet the direction and magnitude of these changes remain uncertain. Rarely are these interactions viewed from both a host and parasite perspective. Using both CTmax, or Critical Thermal Maximum, and Clark-type polarographic oxygen microelectrodes, the physiological metabolic effects are investigated. CTmax is examined in Common Shiners, Luxilis cornutus, and is shown to be modulated by parasitism: uninfected fish had the highest CTmax average of 37.0°C, whereas heavily infected fish had decreased CTmax averaging 35°C. Prior to the parasite metabolic investigation, a suitable medium was determined, as …


Environmental Enrichment Preferences And Play Behavior In Commercial Turkey Hens, Paul Dang May 2026

Environmental Enrichment Preferences And Play Behavior In Commercial Turkey Hens, Paul Dang

Poultry Science Undergraduate Honors Theses

Commercial turkey production contributes significantly to the U.S. agricultural industry, yet studies on positive welfare indicators, especially play behavior, are limited. This study evaluated turkey hen interactions with two environmental enrichments (straw bales and pecking stones hung from the ceiling) and the expression of play behavior in turkey barns. Three commercial turkey hen barns were equipped with environmental enrichments and time-lapse video cameras. Play behaviors were recorded during transect walks in two barns using 30-second scans of disturbed and undisturbed patches. Regional use of space around enrichments was quantified through region-of-interest (ROI) analysis of time-lapse images collected from days 16-31 …


Transcriptomic Responses Of Teleost Fishes To Thermal And Environmental Stress: A Synthesis Of Rna-Seq Studies, Aleen Ammar May 2026

Transcriptomic Responses Of Teleost Fishes To Thermal And Environmental Stress: A Synthesis Of Rna-Seq Studies, Aleen Ammar

Theses, Dissertations and Culminating Projects

Climate change is driving rapid shifts in aquatic ecosystems, increasing the frequency and intensity of environmental stressors that affect survival, species distributions, and productivity. Alongside increases in temperature, stressors, such as hypoxia and acidification, are also intensifying with climate change. Teleost fishes exhibit substantial variation in tolerance to such environmental stressors. These range from specialists with narrow limits to generalists capable of persisting across fluctuating conditions, complicating predictions of vulnerability under ongoing environmental change. Transcriptomic approaches, particularly RNA sequencing (RNA-seq), provide a powerful approach for linking environmental stressors to physiological responses by quantifying genome-wide changes in gene expression. To assess …


Assessing Decadal Changes In Eelgrass Communities From Barnegat Bay, Nj, Janelle Marie Delcastillo May 2026

Assessing Decadal Changes In Eelgrass Communities From Barnegat Bay, Nj, Janelle Marie Delcastillo

Theses, Dissertations and Culminating Projects

Zostera marina (eelgrass) is a widely distributed marine angiosperm across northern hemisphere coastlines, yet many regions have experienced substantial declines due to disease, coastal development, and other anthropogenic stressors. Barnegat Bay is the only estuary in New Jersey that still supports extensive eelgrass beds, although it has undergone notable losses over the past five decades. To evaluate decadal-scale ecological change, we collected sediment cores in 2025 from a site with historical data spanning 2001–2010. Overall faunal densities remained relatively consistent, but several taxa exhibited clear shifts in abundance. Amphipods in the Phoxocephalidae and the isopod Erichsonella increased, whereas the cold-affinity …


Shape Variation In The Mandible Of The Borophagine Canids And Implications For Predatory Behavior, Benjamin Neavear May 2026

Shape Variation In The Mandible Of The Borophagine Canids And Implications For Predatory Behavior, Benjamin Neavear

Theses, Dissertations and Culminating Projects

The borophagines are an extinct lineage of canids that inhabited North America for more than 20 million years. This group included genera such as Aelurodon, Borophagus, and Epicyon. Borophagines are commonly referred to as “bone-crushing dogs” because their skull morphology converged with that of extant hyenas, predators capable of consuming bone. The purpose of this study was to determine whether convergence was present in the mandible, an anatomical structure strongly correlated with feeding behavior, particularly in the genera Aelurodon and Borophagus. It was hypothesized that most borophagine taxa possessed mandibular shapes similar to those of hyenas. Numerous mandibular characters were …


At The Intersection Of Science And Sustainability: A Qualitative Study Of Food Scientists' Personal And Professional Perspectives, Srushti Parikh May 2026

At The Intersection Of Science And Sustainability: A Qualitative Study Of Food Scientists' Personal And Professional Perspectives, Srushti Parikh

Theses, Dissertations and Culminating Projects

Sustainable food systems require not only technological innovation but a more profound understanding of the professionals involved in the field. Even though the topic of sustainability is currently gaining importance throughout the food industry, the real expertise of food scientists is under-researched. PURPOSE: The purpose of this study is to explore food scientists' perspectives on sustainability, how these perceptions influence the way they pursue their careers, and how they view their impact in the larger sustainability agenda of the food industry. METHODS AND MATERIALS: This study captured detailed insights of 22 food scientists’ perspectives through qualitative methods. Food scientists in …


Evaluation Of Flt3 Inhibition With Quizartinib During Busulfan Conditioning For Hematopoietic Stem Cell Transplantation, Catherine Jankovic May 2026

Evaluation Of Flt3 Inhibition With Quizartinib During Busulfan Conditioning For Hematopoietic Stem Cell Transplantation, Catherine Jankovic

Biochemistry and Molecular Biology

Hematopoietic stem cell transplantation (HSCT) is one of the only curative therapy options for many hematologic malignancies. HSCT relies on effective conditioning regimens to deplete host hematopoietic cells and enable donor engraftment. The alkylating agent Busulfan is widely used in myeloablative conditioning strategies due to its potent cytotoxic effects on hematopoietic cells and ability to clear the bone marrow. However, optimization of conditioning strategies remains an important area of investigation to improve transplant outcomes and reduce toxicity. Preliminary studies have shown that the FLT3 inhibitor Quizartinib synergizes with some chemotherapeutic agents, suggesting that targeted inhibitors may enhance conventional conditioning regimens. …


Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier May 2026

Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Honors Theses

This study explores how the tempo of music, measured in beats per minute (BPM), affects dogs' heart rate and oxytocin levels, adding to past research that shows that music affects physiological and behavioral states in dogs. Being able to understand these effects holds practical implications for improving overall well-being in canines in environments such as homes, shelters, and clinical environments. Using a within-subjects design, the dogs were exposed to slow and fast versions of one instrumental track, along with a white noise baseline condition before each randomized condition. Heart rate was recorded continuously throughout the session, and oxytocin saliva samples …


Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza May 2026

Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Specialty crops such as vegetables are some of the most susceptible to yield losses and increased management difficulty over time due to pests. One of the potential solutions to this problem, which aims at ensuring the sustainability of the sector, is using conservation biological control practices such as intercropping to increase diversity, while maintaining the economic feasibility and profitability of the system. This study was conducted to evaluate the impact of this management practice using Bell pepper (Capsicum annuum var. King Arthur) as the main crop, intercropped with flowers including zinnia (Zinnia elegans var. Benary’s Giant), sunflower ( …


Clinical And Demographic Characteristics Of Hospitalized Patients With Xylazine-Related Wounds, Elizabeth Novick, Nikhil Seval, Dagan Coppock, Mollie Tucker, Daniel Taupin, John Zurlo, Carolyn Kramer May 2026

Clinical And Demographic Characteristics Of Hospitalized Patients With Xylazine-Related Wounds, Elizabeth Novick, Nikhil Seval, Dagan Coppock, Mollie Tucker, Daniel Taupin, John Zurlo, Carolyn Kramer

Department of Medicine Faculty Papers

OBJECTIVES: Little is known about the demographic and clinical features of patients with xylazine-related wounds. We sought to characterize hospitalized patients with substance use disorder (SUD) and xylazine-related wounds seen for infectious diseases consultation.

METHODS: We retrospectively reviewed the medical records of 193 patients diagnosed with SUD at 3 Philadelphia hospitals between October 2023 and January 2024. Patients were identified as either being diagnosed with (n=73) or without (n=120) xylazine-related wounds. We compared the demographic and clinical features between the 2 groups.

RESULTS: Patients diagnosed with wounds were younger ( P < 0.001), more likely to be White or Hispanic and less likely to be Black/AA ( P < 0.001), more likely to have tested positive for fentanyl ( P < 0.001), amphetamines ( P < 0.001), and cocaine ( P 0.001), more likely to acknowledge injection ( P < 0.001), and more likely to leave the hospital by patient-directed discharge (PDD) ( P < 0.001). Most patients had multiple wounds (75%), and more than half (53%) had at least one wound greater than 10 cm at its widest dimension. Although most patients diagnosed with wounds received antibiotics, many did not have symptoms or signs of infection.

CONCLUSIONS: Xylazine-related wounds are highly associated with illicitly manufactured …


Developing Aero-Floating Treatment Wetland (Aeroftw) Scaffolds: A Non-Plastic Alternative To Conventional Plastic-Based Ftws, Ada C. Montoya Gomez May 2026

Developing Aero-Floating Treatment Wetland (Aeroftw) Scaffolds: A Non-Plastic Alternative To Conventional Plastic-Based Ftws, Ada C. Montoya Gomez

All Theses

This research was conducted to explore the possibility of constructing floating treatment wetlands (FTWs) without using plastic materials. FTWs are floating platforms that hold plants on the water’s surface, letting roots dangle within the water column, to help remove contaminants from contaminated water, especially in stormwater ponds. Many conventional FTWs are made with plastic components, such as mats and plastic cup containers, which can release microplastics into the environment over time. The goal of this project was to design and test a new type of FTW that does not depend on plastics to support FTW flotation. The study took place …


Disruption Of Energy Homeostasis By Chemical Mixtures Containing Persistent Pollutants In Hepg2 And C2c12 Cells, Morgan Jacobellis May 2026

Disruption Of Energy Homeostasis By Chemical Mixtures Containing Persistent Pollutants In Hepg2 And C2c12 Cells, Morgan Jacobellis

All Theses

The Environmental Influences on Child Health Outcomes Fetal Growth Studies (ECHO-FGS) birth cohort data was used to identify chemical mixtures associated with adverse outcomes. This biomonitoring study associated specific chemical mixtures in maternal serum with low birth weight or childhood obesity (Pearce et al., 2021). Mixture Profiles 1, 3, and 4, comprised of different observed concentrations of DDE, PCB153, BDE47, and PFOS were prioritized. Mixtures were tested to determine whether they perturbed mitochondrial metabolism and energy sensing pathways. The purpose of this study was to elucidate a potential mechanism linking maternal exposure to mixtures containing persistent organic pollutants (POPs) to …


Field Pea (Pisum Sativum L.) Response To Planting Date In South Carolina Organic Cropping Systems, Tristan Lawrence May 2026

Field Pea (Pisum Sativum L.) Response To Planting Date In South Carolina Organic Cropping Systems, Tristan Lawrence

All Theses

Field peas (Pisum sativum L.) are not widely cultivated in South Carolina, resulting in limited agronomic data to support farmers interested in this crop. However, field peas hold significant promise as a cool season crop for both organic and conventional growers in the region. Most commercially available field pea cultivars are evaluated and bred for the Pacific Northwest, where environmental conditions differ substantially from those in the southeastern United States. Consequently, there is a lack of region-specific recommendations for successful cultivation in South Carolina. To address this gap, a two-year planting date study was conducted during the …


Polydactyl Precursors: Insights Into Early Tetrapod Locomotion From Polydactyl American Alligators, Trevor P. Brewington May 2026

Polydactyl Precursors: Insights Into Early Tetrapod Locomotion From Polydactyl American Alligators, Trevor P. Brewington

All Theses

Several of the earliest tetrapod lineages exhibited polydactyl limbs, with seven or eight digits per limb rather than the typical five or fewer seen in extant lineages. How these digit configurations might have been used during terrestrial locomotion is unclear, given the lack of living models in which their performance can be studied and limited evidence from fossil trackways. However, polydactyly occasionally appears in natural populations of American alligators (Alligator mississippiensis), animals with body plans that broadly resemble those of early tetrapods. To test how polydactyl limbs similar to those of early tetrapods might have functioned on land, …


Dissecting The Complex Genomic Landscape Underlying Oxidative Stress Resistance Using Natural Variation In Yeast, Sonali Lakshika Anne Lenaduwe May 2026

Dissecting The Complex Genomic Landscape Underlying Oxidative Stress Resistance Using Natural Variation In Yeast, Sonali Lakshika Anne Lenaduwe

Graduate Theses and Dissertations

Stress is experienced across the tree of life. Survival in fluctuating environments depends on a robust genetic architecture. This dissertation examines the genetic and metabolic determinants underlying one such adaptive response: cross-protection. Leveraging natural variation in the stress responses of budding yeast, Saccharomyces cerevisiae, I examine the genetic variation contributing to ethanol-induced cross-protection against oxidative stress. Acquired stress resistance, in which mild pre-exposure improves survival to subsequent stresses, is prevalent across diverse organisms and can involve both same-stress and cross-protection. Using high-throughput bulk segregant analysis (BSA-seq) and advanced intercross mapping populations derived from natural yeast isolates, I identify quantitative trait …


The Role Of Floodplain Connectivity In Shaping The Abundance, Size-Class Distribution, And Schooling Behavior Of The Associated Fish Assemblage, Adam H. Quade May 2026

The Role Of Floodplain Connectivity In Shaping The Abundance, Size-Class Distribution, And Schooling Behavior Of The Associated Fish Assemblage, Adam H. Quade

LSU New Orleans Theses and Dissertations

The life histories of many Louisiana fishes are closely tied to the timing, magnitude, and duration of the Mississippi River flood pulse, which regulates the ecological connectivity and productivity of adjacent floodplain habitats. Anthropogenic modifications such as levee construction and water-control structures have disrupted these natural hydrologic linkages, altering habitat quality and community structure across the Lower Mississippi River Basin. This dissertation used high-resolution imaging sonar to investigate how fish assemblages, species, and collectives respond to changes in floodplain connectivity within the Richard K. Yancey Wildlife Management Area prior to hydrologic restoration. In the first chapter, I applied imaging sonar …


Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das May 2026

Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das

Graduate Theses and Dissertations

Abstract Here, I report new ultrastructural evidence for multineuronal innervation (many-one) of a single epithelia-muscular cell in sea-anemone cnidarian Nematostella vectensis. Using Serial Block-Face Scanning Electron Microscopy (SBF-SEM) and ImageJ, I found that EMCs are located at the base of ectoderm, with an elongated cell body that includes basal processes, a bilobed nucleus, and myofibrils aligned parallel to the basal surface along with their structural quantification. Notably, I found multiple mechanosensory neurons form synapses with a single EMC. These findings enhance our understanding of cnidarian neuromuscular organization and reveal that coordination between sensory and contractile systems in N. vectensis is …


Creatures Of Habit? Impact Of Chick Feeder Tray Location On Feed Consumption At 0-7 Days Of Age, Emily Kate Green May 2026

Creatures Of Habit? Impact Of Chick Feeder Tray Location On Feed Consumption At 0-7 Days Of Age, Emily Kate Green

Graduate Theses and Dissertations

Early chick nutrition is critical for establishing target growth performance characteristics in broiler chickens. This study evaluated factors within commercial broiler houses that potentially influence feed consumption during the first 7 days. Three trials were conducted in a 12.2 x 122 m commercial broiler house to assess the effects of house sectioning, spatial barriers, and feedline location. In Trial 1, a one-way experimental design was used in which the house was divided into four equal sections using migration fencing every 30.5 m. House sections served as a fixed factor. Feed was provided using chick trays placed beneath the feed line …


A Survey Of The Collembola Of The Buffalo National River Park, Emma Renee Critchfield May 2026

A Survey Of The Collembola Of The Buffalo National River Park, Emma Renee Critchfield

Graduate Theses and Dissertations

Collembola are a neglected and understudied class with few recent U.S surveys. The aim of this thesis was to survey the Buffalo National River for Collembola and create a catalogue of all known species in the state of Arkansas, USA. Leaf litter, mosses, rotting wood, fungi and malaise trap samples were taken from 13 locations within the Buffalo National River park. In total, 14 families, 34 genera and 59 species were identified from 22,028 collected specimens. Of those, 31 species represent new state records. This survey also validates malaise traps as useful tools for Collembola sampling. The malaise trap samples, …


Assessing The Potential Of Taming Chicken Gut Commensal Bacteria Using Random Transposon Mutagenesis, Arjun Chapagain May 2026

Assessing The Potential Of Taming Chicken Gut Commensal Bacteria Using Random Transposon Mutagenesis, Arjun Chapagain

Graduate Theses and Dissertations

The chicken gut microbiome comprises hundreds of bacterial species whose functional roles remain largely unknown, in part because the tools needed to experimentally manipulate these organisms in their native community context have not been systematically developed or validated. This dissertation addresses the gap through three connected research chapters that progressively build a framework for transposon-based genetic delivery in the chicken cecal microbiome, moving from community-wide accessibility mapping to vector optimization to library preparation refinement. In Chapter 2, we established the community-level insertion landscape in chicken cecal metagenome using Himar transposon delivery via RP4 conjugation and aligned Tn-seq reads to a …


Geospatial Science And The Changing Environment: Applied Methods For Sustainable Agriculture And Landscape Management, Harrison Wakefield Smith May 2026

Geospatial Science And The Changing Environment: Applied Methods For Sustainable Agriculture And Landscape Management, Harrison Wakefield Smith

Graduate Theses and Dissertations

Rapid environmental change is disrupting agricultural productivity, ecological function, and the long-term resilience of managed landscapes. At the same time, the rapid growth of geospatial data science has improved our understanding of environmental change and increasingly is being used to improve sustainability in agricultural and environmental management. However, critical gaps remain that limit the applicability of data-driven insights in landscape management. This dissertation investigates the potential of geospatial analytics for sustainable agriculture and landscape management, with a focus on applied methods that operate across spatial and temporal scales. Using field, landscape, regional, and national datasets, it explores the capabilities and …


Aggregation And Collation Of Texas Crayfish Museum Specimens To Determine Their Current Conservation Statuses, Adrian Alvarez May 2026

Aggregation And Collation Of Texas Crayfish Museum Specimens To Determine Their Current Conservation Statuses, Adrian Alvarez

Biology Theses

Crayfish account for over half of the macroinvertebrate biomass in many ecosystems and serve as crucial bioindicators because of their behavioral and physiological adaptability to environmental changes. Despite being home to approximately 52 native crayfish species, Texas currently lacks protective regulations. This gap is primarily because of insufficient information regarding their distribution and occurrences within the state, including their statuses as Species of Greatest Conservation Need (SGCN). This study focuses on compiling and comparing crayfish museum records with current iNaturalist field survey data across Texas to address these knowledge gaps and recommend updates to SGCN statuses. Five species not currently …


Cis-Variant Gene-Expression Association Analysis: A Targeted Approach To Identify Functional Cis-Regulatory Variation, Sofiya Arora May 2026

Cis-Variant Gene-Expression Association Analysis: A Targeted Approach To Identify Functional Cis-Regulatory Variation, Sofiya Arora

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Phenotypic diversity in plants is largely driven by changes in gene function arising from mutations in both the coding and regulatory regions. Regulatory variation is particularly pervasive because it can fine-tune gene expression without disrupting protein function and is therefore less likely to be strongly selected against in natural populations. As a result, it has played a central role in evolution and domestication. In our previous genome-wide eQTL mapping study in maize, nearly two-thirds of the expressed genes were found to harbor detectable cis-expression quantitative trait loci (cis-eQTLs) with strong local effects on gene expression. Though these cis-variants are widespread, …