Digital Commons Network^™

Aprepitant Ameliorates Cyclophosphamide-Induced Testicular Toxicity In Mice Via Suppression Of P38 Mitogen-Activated Protein Kinase, Suzan Awad Abdelghany Morsy, Amira H. Eltrawy, Nehal Adel Khalil, Abd Elaziz Mm, Nourhan Mohamed Mohamed Saeed, Gehad M. Khamis

Mansoura Medical Journal

Background: While cyclophosphamide (CP) is a commonly used immunosuppressant and anticancer drug, it has a high risk of testicular toxicity. The current study evaluated the protective and therapeutic actions of aprepitant (APR), a neurokinin-1 receptor blocker, against CP-induced testicular toxicity in mice and the role of oxidative stress and phosphorylated P38 mitogen-activated protein kinase (p-P38 MAPK) in this action.Methods: Forty male mice were assigned to four groups: control (vehicle only), CP-induced testicular toxicity (single 200 mg/kg intraperitoneal CP dose), APRprotected (20 mg/kg/day APR starting one day before CP, continued for 30 days), and APR-treated (same APR dose starting one hour …

Interplay Between Amiodarone And Statins: Experimental Evidence Of Synergistic Hepatotoxicity, Hager Zaki Elghareb, Abdel-Motaal M. Fouda, Mohamed Mahmoud Elhadidi, Ahmed Abdel-Monem Elmetwally

Mansoura Medical Journal

Background: Drug-induced liver injury (DILI) continues to be a main contributor to acute liver failure. So, this study aimed to investigate interactions between amiodarone and atorvastatin concerning hepatic safety, due to increasing clinical practice of their combination for optimal arrhythmia control. Methods: Forty male Sprague Dawley rats were randomly divided into four groups (10 in each group). Group 1 was control group receiving normal saline. Group 2 received Amiodarone-only \ (administered 30 mg/kg/day orally). Group 3 received Atorvastatin only (administered 10 mg/kg/day orally). Group 4 received combination of both amiodarone and atorvastatin in same previous doses. All drugs were dissolved …

Relationship Between Down Syndrome And Attention Deficit Hyperactivity Disorder And The Role Of Fkbp5 Gene: A Review Of Current Evidence, Israa A. Elsawi, Dina Salama Abd-Elmagid, Doaa Shahin, Mohamed Rizk Khodair, Sohier Yahia

Mansoura Medical Journal

The most prevalent chromosomal disorder is Down syndrome (DS). Neurodevelopmental disorders are highly associated with Down syndrome, such as attention deficit hyperactivity disorder (ADHD). Down syndrome is also distinguished by intellectual disability and developmental delays, with many affected individuals presenting manifestations of ADHD like inattention or hyperactivity. Differentiating ADHD from overlapping DS-related cognitive deficits remains a diagnostic dilemma. The FKBP5 gene, particularly the rs7748266 polymorphism, has been implicated in modulating stress response pathways linked to ADHD susceptibility. This review summarizes the relationship between DS and ADHD and explores the emerging role of FKBP5 rs7748266 in this context. This may later …

Inactivation Of Zika Virus With Hydroxypropyl-Beta-Cyclodextrin, Cory R. Hewitt, Nicholas J. Wixon, Arthur Gallegos, You Zhou, Victor C. Huber, M. Scott. Killian

Nebraska Center for Biotechnology: Faculty and Staff Publications

Background/Objectives: Zika virus (ZIKV) infection is associated with life-threatening diseases in humans. To date, there are no available FDA-approved therapies or vaccines for the specific treatment or prevention of ZIKV infection. Variation in the ZIKV envelope protein (Env), along with its complex quaternary structure, presents challenges to synthetic approaches for developing an effective vaccine and broadly neutralizing antibodies (bnAbs). We hypothesized that beta-cyclodextrin (BCD) could be used to uniquely inactivate infectious ZIKV without disruption of Env.

Methods: ZIKV was propagated in Vero cells and admixed with BCD. The BCD-treated ZIKV was evaluated for infectivity using immunofluorescence and quantitative …

Characterization Of Multispecies Microbial Communities At Beef And Pork Processing Plants And Their Impact On Pathogen Stress Tolerance, Rong Wang, Sapna Chitlapilly Dass, Vignesh Palanisamy, You Zhou, Tatum Katz, Joseph M. Bosilevac

Nebraska Center for Biotechnology: Faculty and Staff Publications

Escherichia coli O157:H7 and Salmonella enterica are major foodborne pathogens. Biofilm formation may potentially contribute to product contamination by these pathogens at meat processing facilities. Further, pathogen stress tolerance may vary significantly due to the interactions with the multispecies microbial community at meat plants, which may be affected by processing activity, animal species, and the local selective pressure caused by sanitization practices.

We characterized natural microorganisms collected from floor drains at various areas at three beef plants and two pork plants and analyzed their impact on pathogen sanitizer tolerance.

The pathogen strains were able to integrate efficiently into the multispecies …

Principles Of Biomedical Research, Faina Linkov, Matthew C. Kostek

Open Education Materials

HLT 430/530

CC BY-NC-SA

Effects Of Starter Cultures On The Metabolite Profile, Antioxidant Activities, And Anti-Aging Properties Of Tapuy Lees, Edward Kevin B. Bragais, Paul Mark B. Medina

Biology Faculty Publications

The Philippine rice wine (tapuy) and tapuy lees making process utilizes bubod, the traditional starter culture. The bubod contains variable number and species of microorganisms and influences the physical and chemical properties of tapuy and tapuy lees, resulting in inconsistent quality products. Hence, improvement and standardization of local starter culture are significant. Here, we examined the effects of well-defined starter cultures (Rhizopus oryzae (RO), Mucor indicus (MI), and Saccharomyces cerevisiae (SC)) and bubod on the metabolite profile, antioxidant, and anti-aging activities of tapuy lees. Our results showed the presence of polyphenols (102.36 ± 2.84 to 173.92 …

Investigating Plk1 In Pulmonary Fibrosis, Tempany Arbogast

Theses and Dissertations--Toxicology and Cancer Biology

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease, with a median survival of only 2–3 years following diagnosis. In the United States, the disease contributes to more than 40,000 deaths annually, underscoring its severe clinical burden. Alveolar type II (AT2) lung epithelial cells serve as progenitors that maintain alveolar integrity by replenishing epithelial populations and producing surfactants, while fibroblasts and myofibroblasts regulate the extracellular matrix and structural support. In pulmonary fibrosis (PF) injury to AT2 cells promotes cytokine and growth factor release, driving inflammation, fibroblast proliferation, myofibroblast accumulation, and excessive extracellular matrix deposition. Aberrant AT2 cell …

Movement And Activity Of Burbot, (Mizay; Lota Lota) In A Spring-Fed Minnesota Lake, Abigail M. Fountain

Biology Graduate Theses

Burbot (Mizay; Lota lota) are a relatively unmanaged freshwater fish, important in many local Indigenous cultures, but often overlooked or maltreated in research, conservation, and fishing practices. As such, relatively little research has been conducted on Burbot, despite ecological significance as an indicator species. A recent resurgence in popularity as a target species, however, has prompted new research to inform management policies. The objectives of this study were to summarize Burbot movement by identifying patterns in swimming activity and identify possible predictors of the movement observed. This study investigated the movements of 39 individuals implanted with acoustic transmitters in Bad …

An Analysis Of Htt Involvement In Huntington’S Disease, Elaine Vanterpool, Micah Andrews

Student Posters

Huntington's disease is a neurodegenerative disorder characterized by loss of striatal neurons. These striatal neurons are essential in cognition, motor function, decision-making, motivation, reinforcement, and reward perception. Affected individuals may show symptoms such as isolation, lack of motivation, depression, mood swings, and personality changes. This study’s purpose is to analyze and highlight the variants of the HTT gene in the pathogenesis of Huntington’s disease.

Hypothalamic Paraventricular Nucleus G⍺Q/G⍺S And Rgs2 Signaling Pathways Mediate Sympathoexcitation And Sodium Retention In Hypertension, Shane Harold Boomer

Dissertations and Theses

The hypothalamic paraventricular nucleus (PVN) regulates diverse physiological processes for maintaining homeostasis, modulating functions ranging from fluid balance to sympathetic nervous system activity. Due to its integral connections with various autonomic and endocrine systems, the PVN significantly influences cardiovascular and renal function, notably in blood pressure homeostasis and the development and regulation of hypertension. The regulatory capacity of PVN is mediated through several signaling pathways, including those involving G-protein coupled receptor (GPCR) and G- protein signaling. We hypothesized the altered PVN signaling through Gαq/Gαs and regulators like regulator of G-protein signaling 2 (RGS2) pathways affected sympathetic nerve activity and blood …

Novel Infection By Mucor Hiemalis Kills Caenorhabditis Hosts Through Intestinal Perforation, Jay Ni

West Chester University Graduate Theses, Dissertations, and Final Projects

The nematode Caenorhabditis elegans has emerged as a popular model system to investigate cell biology and host-pathogen interactions. Presently, C. elegans is studied as a natural host of intracellular pathogens such as microsporidia and Orsay virus along with extracellular bacterial and fungal pathogens. The use of C. elegans as a model in host-pathogen research is limited by the number of naturally occurring pathogens to the organism. Through a sampling project to identify new pathogens of C. elegans, we identified the fungus Mucor hiemalis as a pathogen of Caenorhabditis species. We observed the fungus in the intestinal lumen of wild-caught …

Estimating The Amount Of Carbon Dioxide Gas Removed From The Atmosphere By The Bryant Park Tree Population, Edimarlyn Gonzalez

Open Educational Resources

No abstract provided.

Least Tern Abundance, Nesting Productivity, And Dispersal Between Nesting And On- And Off-River Foraging Sites In The Missouri National Recreational River, Kyle Jorgensen

Dissertations and Theses

The Interior Least Tern (Sterna antillarum athalassos) has experienced significant conservation success, culminating in its removal from the federal endangered species list in 2021. However, the species remains state-endangered in South Dakota, and knowledge gaps persist regarding its foraging ecology and population dynamics along the Missouri National Recreational River (MNRR). This study aimed to address these gaps by quantifying Least Tern abundance, nesting productivity, and movement patterns between nesting and foraging sites during the 2024 and 2025 breeding seasons. We conducted surveys of Least Terns on riverine sandbars and off-river wetlands within a 30-km radius of the MNRR, employing both …

Forage News [2025-01], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-02], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-12], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-11], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-10], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-09], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-06], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-05], University Of Kentucky

Forage News

No abstract provided.

Forage News [2025-04], University Of Kentucky

Forage News

No abstract provided.

Influences Of Sex, Alcohol, And Stress On Conditioned Fear Responses, Heyam K. Mayberry

MUSC Theses and Dissertations

Learned fear responses are acquired through the identification and association of threatening stimuli with aversive outcomes. Fear responding and the proper maintenance of defensive behaviors is integral to survival. However, without the homeostasis of such systems, fear responses can become maladaptive and lead to dysregulated behavioral responding. Psychological conditions such as alcohol use disorder (AUD) and post-traumatic stress disorder (PTSD) often lead to impairments in fear learning, deficits in fear memory, and heightened fear responses. While there are clear sex differences in the prevalence of both AUD and PTSD, development of either disorder increases the risk for comorbid AUD/PTSD, leading …

Astrovascular Decoupling In Awake 5×Fad Mice Is Associated With Reduced Astrocytic Calcium, Ruei-Lung Lin, Sophiya L. Sims, Nicholas A. Wright, Léopoldine B. Galopin, Blaine Weiss, Susan D. Kraner, Jacquelyn E. Rhinehart, Ting-Hsuan Lu, Pradoldej Sompol, Christopher M. Norris, Olivier Thibault

Pharmacology and Nutritional Sciences Faculty Publications

INTRODUCTION

Evidence points to dysregulated Ca²⁺ in neurons and astrocytes in models of amyloidosis. While most of these data were obtained in vitro or in vivo under anesthesia, less work has investigated these variables in awake ambulating mice.

METHODS

Astrocytic Ca²⁺ fluctuations (GCaMP8f) were imaged concomitantly with vasoreactivity in S1 on a two-photon microscope during rest and ambulation. Single-cell resolution variables were extracted using continuous wavelet transform and traditional ΔF/F measures.

RESULTS

Along with increases in amyloid beta (Aβ) accumulation, we found significant reductions in measures of astrocyte functional connectivity, pairwise correlations, and network synchronicity in older 5×FAD …

Artificial Insemination Of Beef Heifers With Multi-Sire Semen, Dempster Christenson, Jordan M. Thomas, Daniel J. Kelly, Richard N. Funston

Nebraska Beef Cattle Reports

This study compared pregnancy rates of beef heifers artificially inseminated with multi-sire semen to single-sire semen. It was hypothesized pregnancy rates resulting from multi-sire semen would be increased compared to single-sire semen. Heifers were artificially inseminated with semen from one of three single-sires or semen from a combination of the same three sires. Pregnancy rates did not significantly differ. Paternity testing suggests sire parentage can be unequal when semen is mixed from multiple sires. In summary, producers can expect similar pregnancy rates using single-sire and multi-sire semen, but progeny may have unequal sire representation.

Impact Of Overwinter Gain On Growth And Reproductive Performance In March-Born Heifers, Josie N. Crouch, J. Travis Mulliniks, Jacki A. Musgrave, Kacie L. Mccarthy

Nebraska Beef Cattle Reports

Summary with Implications

Heifers developed to lower rates of overwinter gain may reduce development costs without growth and reproduction impacted. A 3-year study was conducted to evaluate different overwinter rates of gain on heifer growth rates, reproductive, and economic performance. Heifers were managed together and individually supplemented (January-May) with dried distillers grains to achieve either a low (0.75 lb/d) or high (1.50 lb/d) average daily gain. At breeding, high gain heifers were heavier than low gain heifers. Average daily gain and body condition score were similar between treatments. Overwinter treatment did not impact attainment of puberty, pregnancy rates, or the …

Impact Of Pre- And Post-Breeding Supplementation On Performance Of May-Born Heifers, Josie N. Crouch, J. Travis Mulliniks, Jacki A. Musgrave, Kacie L. Mccarthy

Nebraska Beef Cattle Reports

Summary with Implications This 3-year study compared growth, reproductive performance, and economics of May-born yearling heifers that either received 2.00 lb/ heifer/ per d of a dried distillers grain supplement from pre-breeding (July) through breeding (August) or no supplement. Supplementation was offered approximately 24 days before breeding, averaging 55 days total. Heifer body weight was unaffected by supplementation. Supplementation increased average daily gain until pregnancy diagnosis, but no differences were noted after pregnancy diagnosis. Providing dried distillers grain increased the percentage of heifers that were cycling before breeding; however, no differences in pregnancy rates, percent that gave birth in the …

Interaction Of Backgrounding System And Implant Use On Growing Calf Performance, Sydney T. Vanderhoff, Braden C. Troyer, Rebecca L. Mcdermott, Mitchell M. Norman, Brandon L. Nuttelman, Galen E. Erickson, Mary E. Drewnoski, James C. Macdonald

Nebraska Beef Cattle Reports

Summary with Implications

A two-year study utilized 480 weaned steers each year targeted to gain either 1 or 2 lb/d during the winter followed by ad libitum forage intake during the summer to determine the effects of winter rate of gain and implant strategy during the winter backgrounding and summer phases on steer performance, forage intake, and compensatory gain. During the winter, steers received a Revalor-G or no implant. In the summer, steers either received a Revalor-IS or no implant. The use of Revalor-G and increased rate of gain during the winter backgrounding phase had additive effects to increase animal …

Quantifying Cereal Rye Pastures Biomass With Image Analysis, Pedro Henrique J. Fernandes, Shelby L. Davies-Jenkins, Biquan Zhao, Wei‑Zhen Liang, Mary E. Drewnoski, Yijie Xiong

Nebraska Beef Cattle Reports

Summary with Implications

By accurately assessing the amount of forage available, producers can estimate the pasture carrying capacity to make informed decisions on management. To provide producers with a feasible and efficient approach to estimate forage mass, this project evaluated performance of two image analysis tools, Crop Canopy Image Analyzer (CCIA) and Canopeo, in estimating forage mass for cereal rye. Forage mass measured by clipping the forage (areas with 5.625 ft²) was correlated with plant cover analyzed by the two tools using photos of the forage taken 37 in above the ground. Results obtained have supported the feasibility …