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Examining The Probiotic Potential Of Pseudoalteromonas Rubra Situated In The Scholarly And Public Spheres, Sam Ruemmler Jan 2025

Examining The Probiotic Potential Of Pseudoalteromonas Rubra Situated In The Scholarly And Public Spheres, Sam Ruemmler

Honors Senior Capstones and Theses

Climate change is affecting our oceans negatively in many ways, such as rising temperatures and increased disease outbreaks. While there are many diseases that are affecting marine organisms, bacteria of the genus Vibrio are present globally and are known to favor warmer waters, which means increasing temperatures will cause an uptick in infections. One species, V. coralliilyticus, has been identified as a pathogen responsible for coral disease and death as well as mortality in rainbow trout, brine shrimp, and Pacific oyster larvae. A possible solution to the rising prevalence of disease is the use of probiotics. One such probiotic candidate …


Investigating Drivers In Phytoplankton Between Lentic And Lotic Ecosystems Within Twelvepole Creek Watershed, Devin Lee Farmer Jan 2025

Investigating Drivers In Phytoplankton Between Lentic And Lotic Ecosystems Within Twelvepole Creek Watershed, Devin Lee Farmer

Theses, Dissertations and Capstones

Harmful Algal Blooms (HABs) threaten aquatic ecosystems, local economies, and water quality. While extensively studied in lakes (lentic systems), their impact on flowing waters (lotic systems) remains underexplored. This study examined algal drivers in both ecosystems within the same watershed, focusing on Beech Fork Lake (lentic) and Twelve Pole Creek (lotic). Key environmental factors, including pH, turbidity, dissolved oxygen, and nutrient levels, were measured using in situ sensors and laboratory analysis. Water samples were analyzed for nitrogen, phosphorus, and algal biomass for comparison to environmental conditions. Phytoplankton was preserved for identification in future research. By comparing these environments, this study …


Perspectives On Using Fats And Oils In Poultry Production, Brian J. Kerr Jan 2025

Perspectives On Using Fats And Oils In Poultry Production, Brian J. Kerr

Proceedings of the Arkansas Nutrition Conference

These proceedings describe lipid composition of fats and oils and outline fatty acid quality measurements. In addition, fat and oil energy composition and digestibility are presented. Finally, these proceedings describe ways to enhance lipid digestibility with emulsifiers and preventing lipid peroxidation with antioxidants.


Sustainable And Profitable Broiler Nutrition, Sergio R. Fernandez Jan 2025

Sustainable And Profitable Broiler Nutrition, Sergio R. Fernandez

Proceedings of the Arkansas Nutrition Conference

These proceedings accompany a presentation on optimizing protein nutrition of broilers while minimizing environmental impacts of nitrogen excretion. The continual advancement of muscle development in modern broilers requires constant alignment of amino acid needs. These proceedings outline the importance and progression of feed grade amino acid supplementation in broiler nutrition.


Net Energy: The Precision Path To Sustainability, P. Srichana, A. C. Tay-Zar, M. Wongphatcharachai, C. Chanseyha Jan 2025

Net Energy: The Precision Path To Sustainability, P. Srichana, A. C. Tay-Zar, M. Wongphatcharachai, C. Chanseyha

Proceedings of the Arkansas Nutrition Conference

Adaptation of the net energy system in broiler diets will increase least cost formulation flexibility. These proceedings outline a net energy system developed and implemented in practice. In addition to lower carbon and nitrogen inputs in dietary formulation, the net energy system reduced feed costs.


Nebline, January/February 2025 Jan 2025

Nebline, January/February 2025

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2024 Extension Highlights

4-H Engaged 34,177 Youth

Nebraska Extension’s Strategic Direction

Reflflection, Growth and Whole-Person Wellness for 2025. Emily Gratopp

Recipe of the Month: Slow Cooker Chicken Tortilla Soup, Emily Gratopp

Extension’s 2025 Master Gardener Volunteer Training Begins Soon

Garden Guide: Things to Do This Month

Upcoming Green Industry Conferences

ProHort Lawn and Landscape Update

2025 Pesticide Applicator Trainings

Crop Production Clinics

Leaving a Lasting Legacy for Midwestern Farm Women

At Ag Literacy Festival, Students Learn How Agriculture Impacts Their Daily Lives

Managing Hard Water, Becky Schuerman

Don’t Let Fungus Gnats Bug You and Your Plants, Kait Chapman

2025 Lincoln …


The Impact Of Large, Manipulated Rainfall Events On Soil Greenhouse Gas Fluxes Is Mediated By Warming, Spatial Heterogeneity And Hurricane Disturbance Recovery In A Puerto Rican Wet Tropical Forest, Nibia Becerra Santillan Jan 2025

The Impact Of Large, Manipulated Rainfall Events On Soil Greenhouse Gas Fluxes Is Mediated By Warming, Spatial Heterogeneity And Hurricane Disturbance Recovery In A Puerto Rican Wet Tropical Forest, Nibia Becerra Santillan

Biology Honors Projects

Substantial uncertainty surrounds how terrestrial ecosystems may respond to the interaction of multiple global change drivers, especially the interaction of atmospheric warming and more frequent or severe extreme weather events. Climate models suggest that the Caribbean may experience more frequent and/or heavier rainfalls under future climate, which is likely to also interact with increased atmospheric temperatures. In this thesis, I investigate how long-term (“press”) soil moisture and temperature conditions interact with acute (“pulse”) extreme precipitation events to influence CO₂ fluxes, labile carbon, and microbial carbon in a wet tropical forest in Puerto Rico. I conducted a fully-factorial soil incubation experiment …


The Cdk8 Kinase Module: A Novel Player In The Transcription Of Translation Initiation And Ribosomal Genes, Brittany Friedson, Stephen D Willis, Natalia Shcherbik, Alicia N Campbell, Katrina F Cooper Jan 2025

The Cdk8 Kinase Module: A Novel Player In The Transcription Of Translation Initiation And Ribosomal Genes, Brittany Friedson, Stephen D Willis, Natalia Shcherbik, Alicia N Campbell, Katrina F Cooper

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Survival following stress is dependent upon reprogramming transcription and translation. Communication between these programs following stress is critical for adaptation but is not clearly understood. The Cdk8 kinase module (CKM) of the Mediator complex modulates the transcriptional response to various stresses. Its involvement in regulating translational machinery has yet to be elucidated, highlighting an existing gap in knowledge. Here, we report that the CKM positively regulates a subset of ribosomal protein (RP) and translation initiation factor (TIF)-encoding genes under physiological conditions in Saccharomyces cerevisiae. In mouse embryonic fibroblasts and HCT116 cells, the CKM regulates unique sets of RP and TIF …


Evolution Of Chitinases In Amphibians: A Multi-Faceted Group Of Enzymes With Dual Function In Digestion And At The Forefront Against Fungal Infections, Devashri Narayan Jan 2025

Evolution Of Chitinases In Amphibians: A Multi-Faceted Group Of Enzymes With Dual Function In Digestion And At The Forefront Against Fungal Infections, Devashri Narayan

Theses and Dissertations

Chitinases are essential enzymes found in living organisms such as amphibians, and they ensure that amphibians survive in their varied ecosystems. They are crucial in the digestion and immunity of most organisms, including plants and animals. Chitinases’ diversity of functions is a testament to their evolutionary adaptability. The primary function of chitinases is the breakdown of chitin, a complex polymer of saccharides often found in the exoskeleton of insects and the walls of fungi. Amphibians that feed on insects make use of chitinases to hydrolyze chitin into simpler sugars that can be easily absorbed to extract the necessary nutrients from …


Improving The Safety Of N,N-Dimethylacetamide As A Potential Treatment For Preterm Birth Using A Vaginal Nanoformulation, Asad Mir Jan 2025

Improving The Safety Of N,N-Dimethylacetamide As A Potential Treatment For Preterm Birth Using A Vaginal Nanoformulation, Asad Mir

Theses and Dissertations

With no available treatment for preterm birth, the field of reproductive medicine has been repurposing small molecule candidates into vaginal nanoformulations as potential treatments for the condition. The commercial solvent N,N-Dimethylacetamide (DMA) has previously been shown to delay and prevent inflammation-induced preterm birth in a mouse model. A vaginal nanoformulation of DMA loaded into a self-nanoemulsifying drug delivery system (SNEEDS) was studied to see if it can also be efficacious. A side by-side histological comparison was done in the more pertinent tissues of the reproductive tract between the traditional formulation of DMA given intraperitoneally (IP) and the vaginal nanoformulation of …


Ms Environmental Biology Capstone Project, Emily A. Patton Jan 2025

Ms Environmental Biology Capstone Project, Emily A. Patton

Regis University Student Publications (comprehensive collection)

This is a masters Thesis for MS in Environmental Biology containing four chapters including a literature review, grant proposal, journal manuscript, and stakeholders report on a current environmental issue. (1) Noise Pollution and Avian Communication in Urban Ecosystems: When the Natural World Struggles to Hear Itself. Urban noise masks essential bird vocalizations, which harms avian fitness. Most species are displaced, and those few urban birds which counteract this noise with song adaptations likely experience trade-offs. America's noise policy remains undeveloped, threatening the health of both humans and avian wildlife (2) Application of Noise Barrier Technology for the Restoration of Urban …


Aligning Exons, Introns, And Flanking Regions Of Cyc And Cuc Genes In Lonicera (Caprifoliaceae), Mark Mordukhay Jan 2025

Aligning Exons, Introns, And Flanking Regions Of Cyc And Cuc Genes In Lonicera (Caprifoliaceae), Mark Mordukhay

Theses and Dissertations

The genus Lonicera (Caprifoliaceae) exhibits great diversity in floral symmetry, petal fusion, and organ arrangement, making it an ideal system for exploring the genetic basis of morphological evolution. We focus here on the roles of CYC and CUC gene families, (CYC2A, CYC2B, CYC3A, CYC3B, CUC2A, and CUC2B) and their potential contributions in shaping floral symmetry and organ fusion. The data set included nucleotide sequences from over 90 Lonicera species, extracted from samples collected from natural habitats or herbaria and then sequenced. A custom bioinformatics pipeline was developed in R using Bioconductor packages to automate BLAST searches, filter results, scan for …


Evaluating Genetic Variation Of Brassica Tournefortii Gouan Collections Via Microsatellite Marker Analysis, Brian Alfaro, Thomas F. Turner, Diane L. Marshall Jan 2025

Evaluating Genetic Variation Of Brassica Tournefortii Gouan Collections Via Microsatellite Marker Analysis, Brian Alfaro, Thomas F. Turner, Diane L. Marshall

Aliso: A Journal of Systematic and Floristic Botany

Plants can differ in phenotypes, but genetic variation may not always reflect phenotypic differentiation. In this study, we tested if native, invasive and landrace population types of Brassica tournefortii Gouan diverged genetically. We performed this comparison by genotyping individuals derived from our own field collections (invasive populations) and from USDA accessions (plants from the native range and landraces). We used two polymorphic microsatellite loci to analyze allelic diversity (Question 1), genetic differentiation, admixture, inbreeding (Question 2) and evolutionary relationships (Question 3) of three population types and of 17 populations of B. tournefortii. Comparison of allelic diversity indices and observed …


Soil Amendments Alter Tree Growth And Wood Decay After Forest Thinning, Deborah S. Page-Dumroese, Martin Jurgensen, Chris Miller, Joanne M. Tirocke, Derek N. Pierson, Cole Mayn, Mark J. Kimsey, Haley C. Anderson Jan 2025

Soil Amendments Alter Tree Growth And Wood Decay After Forest Thinning, Deborah S. Page-Dumroese, Martin Jurgensen, Chris Miller, Joanne M. Tirocke, Derek N. Pierson, Cole Mayn, Mark J. Kimsey, Haley C. Anderson

Michigan Tech Publications

Forest soil amendments are increasingly used in western US forests to dispose of unmerchantable woody residues, reduce wildfire risk, and improve soil properties. Our objective was to determine the effect of fertilizer and organic amendments on tree growth and organic matter decomposition after thinning. Treatments were a control, three single soil amendments (wood chips, fertilizer, and biochar), and one combined soil amendment (biochar + fertilizer), each applied after thinning a ponderosa pine (Pinus ponderosa Dougl. ex. Laws) stand. After 10 years, amendment treatments had no effect on tree diameter increment (p = 0.600), but the biochar + fertilizer and wood …


An In Vivo Study Of Lns8801, A Gper Agonist, In A Spontaneous Melanoma-Prone Mouse Model, Tgs., Christina Marinaro, John Sauer, Christopher A Natale, Todd Ridky, Suzie Chen Jan 2025

An In Vivo Study Of Lns8801, A Gper Agonist, In A Spontaneous Melanoma-Prone Mouse Model, Tgs., Christina Marinaro, John Sauer, Christopher A Natale, Todd Ridky, Suzie Chen

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Melanoma is the most aggressive and deadly form of skin cancer that arises from the transformation of melanocytes, the pigment producing cells of the skin. In the year 2024 there will be approximately 10,000 new cases of melanoma diagnosed and approximately 8,000 deaths attributed to melanoma in the United States. In this study we treated a group of male and female transgenic mice that spontaneously develop metastatic melanoma, TGS, with a G-protein-coupled estrogen receptor agonist LNS8801 to assess the efficacy on disease progression. A second group of male and female TGS mice was also exposed to UVB irradiation to mimic …


The Effect Of Two Common Herbicides On The Growth And Development Of Two Anuran Species (Boreorana Sylvatica And Dryophytes Chrysoscelis), Jerica Eaton Jan 2025

The Effect Of Two Common Herbicides On The Growth And Development Of Two Anuran Species (Boreorana Sylvatica And Dryophytes Chrysoscelis), Jerica Eaton

Masters Theses & Specialist Projects

Amphibian populations have been declining globally since the 1950s. Many species that have experienced declines are viewed as 'environmental indicators' that reveal habitat degradation. Declines can be attributed to several factors, including chemicals used in agriculture, such as Roundup and Atrazine. Agrochemicals contaminate water bodies through runoff or leaching and cause alterations to aquatic ecosystems. This study evaluated the effects of herbicide usage on two wild anuran populations using wood frogs (Boreorana sylvatica) and Cope’s gray tree frogs (Dryophytes chrysoscelis) as a model. We determined the effect of the herbicides at two concentrations, a high concentration …


Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos Jan 2025

Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos

Regis University Student Publications (comprehensive collection)

1) This review investigates the relationship between pesticide use on golf courses and in agriculture to determine whether the adverse health effects documented in agricultural settings also apply to golf course environments. While the negative health effects from agricultural pesticide use are well established, golf courses use pesticides with greater frequency and intensity, suggesting a potentially higher exposure risk for both workers and players.

2) This study proposes methods to quantify pesticide bioaccumulation on golf courses and adjacent lands. We hypothesize that areas surrounding golf courses will exhibit a spatial gradient of pesticide accumulation, increasing with proximity to treated sites …


Navigating Cultural Differences In Community-Based Conservation: A Malagasy Case Study, Austin J. Cheng Jan 2025

Navigating Cultural Differences In Community-Based Conservation: A Malagasy Case Study, Austin J. Cheng

CISLA Senior Integrative Projects

This SIP explores the tension between avoiding cultural imperialism and remaining true to ideals, particularly of gender equality in the creation and funding of community-based conservation organizations in patriarchal societies. To tether these loftier ideas to practice, I use my internship with Planet Madagascar in Ankarafantsika National Park as a case study, and explore it through the lenses of feminist, pragmatist, and post-colonial philosophers.


Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerald B. Johnson Jan 2025

Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerald B. Johnson

Library/Life Sciences Undergraduate Poster Competition 2025

Does predation environment affect the cognitive abilities of the tropical fish Brachyrhaphis rhabdophora?


Fishing For Neurocircuitry: Identifying Multi-Sensory Integrating Neurons In The Optic Tectum Of Zebrafish, Logan Carson, Arminda Suli, Adeline Hamilton, Logan Carson, Karianne Jex, Erika C. Marks, Jordan T. Yorgason, Tracianne B. Neilsen Jan 2025

Fishing For Neurocircuitry: Identifying Multi-Sensory Integrating Neurons In The Optic Tectum Of Zebrafish, Logan Carson, Arminda Suli, Adeline Hamilton, Logan Carson, Karianne Jex, Erika C. Marks, Jordan T. Yorgason, Tracianne B. Neilsen

Library/Life Sciences Undergraduate Poster Competition 2025

The superior colliculus (SC) is a mammalian midbrain structure involved in multimodal sensory integration and is implicated to have a role in neurodevelopmental disorders. Although the presence of multisensory integrating neurons (MINs) in the SC has been well documented by electrophysiology techniques, little is known about their morphological or molecular characterization. To identify and study MINs, we utilized SC’s non-mammalian homologous structure–the optic tectum (OT)–in the genetically tractable model organism zebrafish. In this process, we generated transgenic lines that allowed for fluorescent detection of neuronal activity by expressing the genetically engineered calcium indicators: cytoRGECO and H2B-jRGECO1a, respectively in mechanosensory hair …


Firing Properties Of Hgvp And Granule Cell Populations In Sensorimotor Pathways, Kate Shepard, M Eyring, Trace Stay Jan 2025

Firing Properties Of Hgvp And Granule Cell Populations In Sensorimotor Pathways, Kate Shepard, M Eyring, Trace Stay

Library/Life Sciences Undergraduate Poster Competition 2025

The vestibular ocular reflex (VOR) functions to keep the eyes stable during head movement. Previous studies have suggested that output from Purkinje cells of the flocculus called HGVP’s modify motor neurons that control the VOR (Kahlon and Lisberger, 2000). (below, Kan, University of Wisconsin)


Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki Jan 2025

Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki

Masters Theses & Specialist Projects

Human-Elephant Conflict (HEC) is an increasingly common event that arises from resource competition due to overlaps in human and elephant environments. Using a conservation behavior approach, the behaviors of elephants can be studied to enhance conservation efforts to mitigate HEC by better predicting which elephants engage in HEC. To understand the factors that affect individual behavioral types of adult Asian elephants (Elephas maximus), my project examined the behavioral patterns displayed by an elephant population in Sri Lanka. I collected demographic and behavioral data of a subset of 15 adult elephants to determine individual differences in five behavioral categories: Affiliation, aggression, …


A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren Jan 2025

A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren

Masters Theses & Specialist Projects

Spider beetles are small, often flightless insects that occur in hyperarid environments around the world. They exhibit interesting morphology and behavior that allows them to conserve water they obtain from plant material, dung, and other detritus. One hyperarid region in which they are found is the Pacific coast of Perú and northern Chile. The Sechura Desert, Peruvian Coastal Desert, and Atacama Desert make up the arid coast where spider beetles occur from north to south. Through multiple years of field work and museum collections, populations of spider beetles were found along the coast in isolated pockets of vegetation, separated by …


The Effects Of Cold Immersion On Motor Consolidation, Manly Barksdale Jan 2025

The Effects Of Cold Immersion On Motor Consolidation, Manly Barksdale

Masters Theses & Specialist Projects

Several physiological mechanisms elicited by exercise have been shown to enhance the encoding of newly learned motor skills. Cold water immersion (CWI) of at least 14°C (57.2°F), a common recovery tactic following exercise, can elicit the upregulation of similar mechanisms (lactate, norepinephrine, and epinephrine). However, little research has explored a relationship between CWI and motor learning. Therefore, the primary purpose of this investigation was to determine the impact of moderate-duration head-out CWI (HCWI) on motor learning measured via Esports tracking task (ETT) and to compare the results to an exercise (EXS) and control (CON) group. Twenty-nine, right-handed, apparently healthy adults …


Diversity And Phylogeny Of Spider Beetles (Ptinidae: Ptininae) In Coastal Chile And Peru, Jacob Bowen Jan 2025

Diversity And Phylogeny Of Spider Beetles (Ptinidae: Ptininae) In Coastal Chile And Peru, Jacob Bowen

Masters Theses & Specialist Projects

Spider beetles of South America have historically received little systematic study, though now they are the subject of recent diversity surveys and documentation. This investigation contributes to the ongoing exploration of spider beetle diversity in South America by adding records for the recently described Cordielytrum and the yet to be described Furcillifer, as well as constructing phylogenies with new genetic material. MaxEnt was used to create an ecological niche model revealing habitat suitability in Chile and Peru for Cordielytrum, a genus of flightless spider beetles currently known only from Peru. Even with few occurrence records, a model with …


Coffee Farmgate Price And Post-Harvest Processing In Honduras., Isaac Desouza Jan 2025

Coffee Farmgate Price And Post-Harvest Processing In Honduras., Isaac Desouza

Theses and Dissertations--Agricultural Economics

Volatile commodity markets and changing climate expose coffee farmers to price and production risk. Proponents recommend specialty coffee as a strategy to combat price risk, because its unique attributes differentiate it from commodity coffee. Extrinsic and intrinsic attributes define specialty coffee and affect prices at many levels of the supply chain. Despite much work on extrinsic attributes, little is known about how intrinsic attributes relate to farmgate price. Post-harvest processing is itself an intrinsic trait and driver of coffee quality. In 2024 we surveyed 261 coffee farmers from La Paz, Honduras, to uncover relationships between capital, post-harvest processing, and farmgate …


Icipe Monthly Digest – Volume 1, Issue 1, January 2025, International Centre Of Insect Physiology And Ecology (Icipe) Jan 2025

Icipe Monthly Digest – Volume 1, Issue 1, January 2025, International Centre Of Insect Physiology And Ecology (Icipe)

icipe Newsletters

No abstract provided.


Learning In Nonleafcutting Fungus-Gardening Ants, Elizabeth D. Boshers Jan 2025

Learning In Nonleafcutting Fungus-Gardening Ants, Elizabeth D. Boshers

Biology Theses

Social complexity influences learning and other cognitive behaviors, but for insects, this influence is not well understood. Delayed rejection is an aversive learning behavior previously studied exclusively in leafcutting ant species, the most socially complex of the fungus-gardening ants. Fungus-gardening ants are unique in that they do not directly consume the material they forage, using it instead as substrate to cultivate a symbiotic fungus on which the ants are obligately dependent. Here, I attempt to induce delayed rejection in several fungus-gardening ant species that exhibit a range of social complexity, then compare the characteristics of delayed rejection behavior among species. …


Acoustic Monitoring Of Oak Toads (Anaxyrus Quercicus), A Longleaf Pine (Pinus Palustris) Ecosystem Endemic Amphibian, Alex Ward Foote Jan 2025

Acoustic Monitoring Of Oak Toads (Anaxyrus Quercicus), A Longleaf Pine (Pinus Palustris) Ecosystem Endemic Amphibian, Alex Ward Foote

Theses, Dissertations and Capstones

The Longleaf pine ecosystem is one of the most endangered ecosystems in the world with only 3% remaining. Many endemic species to the longleaf pine ecosystem are also in decline and suffer from habitat loss resulting from fire exclusion, urbanization, agriculture, and industrial silviculture. Oak Toads (Anaxyrus quercicus) are a longleaf pine ecosystem obligate; the species is experiencing declines across its range and only has protections in Virginia and North Carolina. We used data from an array of Automated Recoding Units across four years (2013, 2014, 2015, 2019) at three state-owned wildlife management areas to capture audio files …


A Metadata Analysis Of Unionid Mussels Of The Greenup Pool Of The Ohio River Using Occupancy Modeling And Species Area Curves, Tyler Mitchell Annarino Jan 2025

A Metadata Analysis Of Unionid Mussels Of The Greenup Pool Of The Ohio River Using Occupancy Modeling And Species Area Curves, Tyler Mitchell Annarino

Theses, Dissertations and Capstones

Although North America contains the largest diversity of freshwater mussels in the world, only ~24% of species in the United States and Canada have stable populations. This group provides unique hurdles for surveying, and current unionid survey protocols include neither longitudinal studies nor randomization of sites. This project conducts a metadata analysis of freshwater mussels in the Greenup Pool of the Ohio River (GPOR) to answer three questions: 1. Does a combination of longitudinal scope, randomization of sites, and historic methodology of surveying result in useful occupancy/detection models (ODMs) of unionids in large streams at the family level? 2. Does …