Digital Commons Network^™

Lineage Tracing And Single-Cell Rna Sequencing Reveal A Common Transcriptional State In Breast Cancer Tumor-Initiating Cells Characterized By Ifn/Stat1 Activity, Eric P Souto, Ping Gong, John D Landua, Ramakrishnan Rajaram Srinivasan, Abhinaya Ganesan, Lacey E Dobrolecki, Stephen C Purdy, Xingxin Pan, Michael Zeosky, Anna Chung, S Stephen Yi, Heide L Ford, Michael T Lewis

Faculty, Staff and Students Publications

A tumor cell subpopulation of tumor-initiating cells (TIC) or "cancer stem cells" is associated with therapeutic resistance, as well as both local and distant recurrences. Signal transducer and activator of transcription (STAT) activity is elevated in TICs in claudin-low models of human triple-negative breast cancer, which enables enrichment of TICs using a STAT-responsive reporter. Lineage tracing of TICs as they undergo cell state changes could enable a better understanding of the molecular phenotypes of TIC and uncover strategies to selectively target TICs. In this study, we developed a STAT-responsive lineage-tracing system and used it in conjunction with the original reporter …

Mapping Mosquito Habitats With Girl Scouts: A Citizen Science Approach Using The “Mosquito Mission: Scout & Report!” Worksheet, Claire A. Klein

Honors Program: Senior Projects (Public)

Mosquitoes, though small, impact millions around the world by being significant vectors of diseases. The search for effective mosquito control efforts is ongoing, however, there is a gap in community involvement with a focus on recognizing and reducing potential breeding areas. Public engagement is a cornerstone of effective mosquito control. This honors thesis investigates the implementation of the Mosquito Mappers program with an original Girl Scout worksheet, “Mosquito Mission: Scout & Report!” This worksheet, which utilizes NASA’s GLOBE Observer Mosquito Habitat Mapper app, enables the Girl Scouts to contribute to real-time environmental monitoring. By guiding Girl Scouts through an inquiry-based …

Hexasodium Fytate (Snf472 Or Csl525) Inhibits Ectopic Calcification In Various Pseudoxanthoma Elasticum And Calcinosis Cutis Animal Models, Miguel Ferrer, Maria Pérez-Ferrer, Marc Blasco, Ida Jacobs, Qiaoli Li, Olivier Vanakker, Lisa Dangreau, Andrea López, Gianluca Malagraba, Firas Bassissi, Joan Perelló, Carolina Salcedo

Department of Biochemistry and Molecular Biology Faculty Papers

Background/Objectives: Ectopic calcification is a pathological condition characterized by the mineralization of soft tissues due to the deposition of calcium phosphate crystals. Hexasodium fytate (CSL525, previously known as SNF472) is a crystallization inhibitor being developed for the treatment of ectopic calcification-related disorders. Our aim was to investigate CSL525 for the treatment of soft-tissue calcification disorders in animal models of pseudoxanthoma elasticum and calcinosis cutis. Methods: In a first study, abcc6^-/- zebrafish larvae were exposed to 1 mM CSL525 for 7 days or kept under the same conditions without CSL525, and spinal mineralization was quantified. In a second study, abcc6 …

Determinants Of Neuronal Viability Following Spreading Depolarization, Michael C. Bennett

Biomedical Sciences ETDs

Spreading depolarizations (SDs) are massive, slowly propagating waves that cause near-complete neuroglial depolarization and are energetically demanding for the brain. Past studies have established SDs as key contributors to secondary injury in tissue that lacks the metabolic supply to meet the energetic burden of repolarization after SD. Efforts to identify the mechanisms that cause deleterious consequences following SD have been ongoing, with significant results showing that blocking excitotoxic levels of Ca²⁺ from entering neurons through NMDA receptors prevents detrimental outcomes. While these studies have advanced the field, other mechanisms may also contribute to the metabolic burden of SD. This …

Results Of The Phase I/Ii Study And Preliminary B-Cell Gene Signature Of Combined Inhibition Of Glutamine Metabolism And Egfr In Colorectal Cancer, Kristen K Ciombor, Seong-Woo Bae, Jennifer G Whisenant, Gregory D Ayers, Quanhu Sheng, Todd E Peterson, Gary T Smith, Kangyu Lin, Saikat Chowdhury, Preeti Kanikarla Marie, Alexey Sorokin, Allison S Cohen, Laura W Goff, Dana B Cardin, John Paul Shen, Scott Kopetz, Cathy Eng, Yu Shyr, Jordan Berlin, H Charles Manning

Faculty, Staff and Student Publications

Purpose: EGFR-targeting mAbs are essential for managing rat sarcoma virus wild-type metastatic colorectal cancer (mCRC), but their limited efficacy necessitates exploring immunologic and metabolic factors influencing response. This study evaluated glutamine metabolism targeting with EGFR inhibition to identify response biomarkers in patients with prior anti-EGFR treatment progression.

Patients and methods: We conducted a phase I/II trial in patients with KRAS wild-type mCRC, combining panitumumab (6 mg/kg) and CB-839 (600 mg/kg or 800 mg/kg), hypothesizing that the dual inhibition of glutamine metabolism and MAPK signaling would enhance outcomes. As study correlatives, we investigated the B-cell activation signature "B-score" and glutamine PET …

The Impact Of Paternal Obesity On Placental Development And Function During Pregnancy, Delaney G. Wolfe, Delaney G. Wiolfe

Department of Nutrition Student Projects

Paternal obesity is a rising concern in conception and reproductive health, with increasing evidence suggesting its impact on pregnancy outcomes. While maternal obesity's effects on fetal development and placental function are well-documented, the role of paternal obesity remains less understood. The placenta, crucial for nutrient exchange and fetal development, can be disrupted by inflammation, oxidative stress, and epigenetic modifications, potentially leading to long-term health implications for both maternal and fetal health. Understanding the paternal influence on placental development is essential for improving pregnancy outcomes and offspring
health. This study aims to investigate the relationship between paternal obesity on placental function …

Genome Analysis Of Aeromonas Hydrophila Strain S14-452, Zarin Tasnim Raya

Undergraduate Research Conference

The Aeromonas hydrophila are gram-negative, opportunistic bacteria that can infect many groups of organisms, mainly fish and amphibians. In channel catfish, A. hydrophila causes fatal bacterial septicemia, leading to massive economic losses for catfish farmers. Therefore, an understanding of its genome will aid in identifying its virulence factors, metabolic pathways and survival mechanisms within the host. This study details the draft genome sequence of A. hydrophila strain S14-452, isolated from channel catfish exhibiting bacterial septicemia during a disease outbreak at a catfish farm.

A. hydrophila strain S14-452 was sequenced using the Illumina iSeq 100 System. Assembly was performed with SPAdes, …

The Role Of Urbanization And Corticosterone In Auditory Sensory Processing In House Sparrows (Passer Domesticus), Brooke Fox, Ashley Lauraine, Hannah Peterson

24th Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2025)

Urban habitat expansion will introduce new anthropogenic challenges for wildlife populations, including habitat fragmentation, novel predators, and pollution. These challenges may hinder animal communication and contribute to increased stress levels. This study will investigate the physiological consequences of stressors on the house sparrow (Passer domesticus). Specifically, we will examine how urbanization affects stress hormone (i.e., corticosterone, CORT) levels and its relationship to auditory processing. It is predicted that urbanization may impose novel stressors on animals, leading to differences in this hormone across urban and rural birds. We seek to understand if differences in CORT levels could impact animals' …

The Impact Of Consuming An Electrolyte Drink Pre Vs Post-Workout On Recovery As Measured Through Urinary Indicators In Collegiate Cross-Country Runners, Laura Taylor, Aidan Becher, Madelynn Struck, Momodou Colley

24th Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2025)

BACKGROUND: Hydration is crucial in sports, as dehydration can decrease strength, endurance, and power. Drinking electrolytes before and after intense exercise can promote hydration. Few studies have compared the difference in consuming electrolytes pre/post-workout. The impact of consuming electrolyte drinks can be assessed by comparing pre/post-workout urinary biomarkers (UB). PURPOSE: To determine if drinking electrolytes before/after a workout improves post-workout recovery as measured by heart rate (HR) and UB in collegiate Cross Country (CC) runners. METHODS: 21 CC runners (43% men, 20±3 years) were divided into four groups. For four weeks (Sep-Oct 2024), participants consumed Liquid IV (LIV) or a …

Foraging Efficiency Of Rural And Urban House Sparrows, Passer Domesticus, Among Natural And Unnatural Obstacles, Annie Waldron, Katie Kowalyk, Mara Gutierrez

24th Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2025)

Urbanization may lead to food displacement for many animals. Urban birds are exposed to man-made objects that can result in variable food locations, unlike rural birds who inhabit areas where food locations are typically consistent. The introduction of unnatural obstacles may affect a sparrow's ability to find and reach food sources. Scientific studies have yet to find a significant increase in problem-solving skills related to foraging in urban versus rural birds. Our research seeks to determine the foraging efficiency of rural and urban house sparrows when exposed to obstructed food sources. Novel objects near a food source may scare birds …

Bird Observations Across Belmont's Campus, Jeremy T. Little, Josiah C. Mustaleski

SPARK Symposium Presentations

Over the course of 10 weeks our team collected data about bird sightings on Belmont’s campus. Sessions were recorded using eBird and Merlin Bird ID to correctly identify the birds and record accurate numbers. We conducted sessions at 3 different spots on campus, each with differing types and amounts of vegetation and human presence. These sessions were 20 minutes in duration and were conducted twice a week at each location. The data gathered was used to gain a better understanding of the bird trends and demographics on campus. This data was also used for WellCore events in conjunction with the …

Analysis Of Psychoactive Compounds In The Web-Building Behavior Of Mangora Maculata, Diane R. Castner

Undergraduate Honors Thesis Projects

The arachnid central nervous system (CNS) functions in an analogous way to the human CNS, despite spiders not having a central brain. Previous studies on spider behavior and web formation have investigated the impacts of stimulants, depressants, sedatives, and hallucinogens, but the impacts of serotonin reuptake inhibitors (SSRIs), such as fluoxetine are unknown. This study examined the impacts of caffeine, nicotine, ethanol, and fluoxetine on the web building behavior of Mangora maculata. Webs were characterized by 9 metrics: web entirety, size of web, radial angles, cell length, number of large holes, frequency of forking of spiral threads, radial angle consistency, …

The Role Of Cholinergic Ventral Pallidum Cells In Effortful Motivation, Elizabeth A. Bien

Dartmouth College Ph.D Dissertations

Maladaptive reward-seeking behavior can be driven by one of several different components including excessive craving elicited by cues that predict rewards, failing to effortfully pursue rewards when it is beneficial to do so, and compulsively seeking rewards when it is no longer advantageous to do so. This thesis includes experiments focusing on these three different components of reward-seeking behavior to better understand how reward-seeking can become maladaptive. First, we wanted to examine the role that sex plays in the acquisition and compulsiveness of sign-tracking behavior in the Long Evans rat, a strain that is commonly used as the background for …

Global And Regional Disparities In Access To Specialist Sarcoma Services, Tomas Zamora, Eduardo Botello, Thomas Jenkins, Charlotte Jeys, Minna Laitinen, Ajay Puri, Lee Jeys, Boom Consensus Meeting Participants, Santiago Abad Repiso, Hesham Abdelbary, Alejandro Abiad Mejia, Ahmed Abood, Juan C Abril Martin, Adesegun Abudu, Ayman Abu Elhamd, Marthelena Acosta, Keisuke Ae, Manish Agarwal, Vivek Ajit Singh, Toru Akiyama, Ibrahim Alshaygy, Jose I Albergo, John Alexander, Patricio A Alfaro, Bugra Alpan, Jose Amaya-Valero, Megan Anderson, Dimosthenis Andreou, Lucas Annabell, Christopher Anthony, Ahmed Aoude, William Aston, Libe Asua Mentxaka, Christine Azzopardi, Thomas Baad-Hansen, Ismail T Badr, Francisco Baixauli-Garcia, Gavin Baker, Tessa Balach, Giacomo G Baldi, Janie Barry, Georges Basile, Stefano Bastoni, Mohammad H Basuki, Henrik Bauer, Lee Bayliss, Ricardo G Becker, Angad Bedi, Joseph Benevenia, Francisco Bengoa, Peter Bergh, Marko Bergovec, Nicholas Bernthal, Odion Binitie, David Boddie, Michele Boffano, Patricia Bonilla Huertas, Anna B Borgognoni, Rajesh Botchu, Richard A Boyle, Jos Bramer, Rahat Brar, Harriet Branford-White, Demien Broekhuis, Samuel E Broida, Tymoteusz Budny, Zachary D Burke, Jorge Cabrolier, Jose A Calvo-Haro, Jorge R Calvo-Tapies, Domenico A Campanacci, Rodrigo Cardoso, Richard Carey Smith, Pedro I Carvallo, Nicolas Casales Fresenga, Jose M Casanova, Oscar Ceballos, Juan L Cebrian Parra, Laura K Certain, Sara Chancon Cartaya, Chung M Chan, Yoon Joo Cho, Peter Choong, Yang-Guk Chung, David Ciechanowicz, David Clever, Sebastian Colina, Luis Consuegra, Cory Couch, Gillian Cribb, Carlos Cuervo, Laurence A Cusick, Solomon Dadia, Tanios Dagher, Dietmar Dammerer, Mark Davies, Nerys Davies, Luis P De La Rosa Martino, Francisco J De Santos De La Fuente, Marieke De Vaal, Claudia Deckers, Javier Delgado Obando, Shaneel Deo, Niklas Deventer, Claudia Di Bella, Gregory Domson, Davide M Donati, Desiree M Dorleijn, Jacques Du Toit, Debra Dunne, Rodolfo Duran Ciarrochi, Walid Ebeid, Elina Ekman, Ahmed M El Ghoneimy, Makoto Endo, Levent Eralp, Mahmoud Etaiwi, Scott Evans, Robin Evrard, Will Eward, Alberto Farese, Peter C Ferguson, Pedro F Ferreira Cardoso, Fabrice Fiorenza, Michael Flint, Hector Flores, Joao Freitas, Bruno Fuchs, Tomohiro Fujiwara, Philipp T Funovics, Marcos Galli Serra, Zakareya Gamie, Carlos Garces-Zarzalejo, Aaron Gazendam, Carsten Gebert, Jasper G Gerbers, Craig Gerrand, Ghaith Abou-Nouar, Michelle Ghert, Kanishka M Ghosh, Max Gibbons, Luis C Gomez-Mier, Jesus Gomez-Vallejo, Anne Gomez-Mascard, Marcos R Gonzalez, Fausto Gonzalez-Lizan, Georg Gosheger, Stuart Goudie, Krista Goulding, Stavros D Goumenos, Anthony Griffin, Ashish Gulia, Sanjay Gupta, Amit Gupta, Maurice Guzman, Mohammed Haitham, Jendrik Hardes, Francisco Hardoy, Yusuf Hasan, Georg Hauer, Helard Havard, Rex Haydon, John Healey, Nerea Hernandez Gonzalez, Adriana Hernandez-Lopez, Asle Hesla, Matthew Hess, Chindanai Hongsaprabhas, Francis Hornicek, Keith Hosking, Matthew T Houdek, Eleanor Houghton, Oluwaseyi K Idowu, Joseph Ippolito, Marc Isler, Shintaro Iwata, Jake Jagiello, Neil Jenkins, Tom Jeys, Luke Johnson, Andy Johnston, Min Wook Joo, Paul C Jutte, Kadri Kaldas, Amar Kamat, Sudhir Kannan, Bilal Kapanci, Zeeshan Khan, Hiroshi Kobayashi, Yehuda Kollender, Sebastian Koob, Daniel Kotrych, Vineet Kurisunkal, Richard Kyte, Jose M Lamo De Espinosa, Alexander L Lazarides, Louis-Romee Le Nail, Pawel Legosz, Burkhard Lehner, Andreas Leithner, Maryse Lejoly, Valerae O Lewis, Peng Lin, Francisco Linares, Santiago A Lozano-Calderon, Ashish Mahendra, Ferdiansyah Mahyudin, Fermin J Mandia Mancebo, Sara Martos Torrejon, Christian Marx, Eric Mascard, Jean-Camille Mattei, Louise Mccullough, Sam Mcmahon, Manuel R Medellin Rincon, Benjamin Miller, Shinji Miwa, Gustavo Molina Uribe, Bryan Moon, Thomas Hilton, Diego J Moriel Garcesco, Carol Morris, Guy Morris, Steward Morrison, Sophie Mottard, Marcio Moura, Linde Muster, Robert Nakayama, Prashant Narhari, Ana Navas, Prakash Nayak, Johannes Neugebauer, Erik T Newman, Jyrki Nieminen, Emmy Nyqvist, Lukas Nystrom, Sarah O'Reilly-Harbidge, Gary O'Toole, Vania Oliveira, André Olivier, Mohamed Omar, Eduardo J Ortiz-Cruz, Harzem Ozger, Korhan Ozkan, Elisa Pala, Emanuela Palmerini, Grant Pang, Panayiotis Papagelopoulos, Giovanni Paraliticci, Michael C Parry, Sam Patton, David Peake, Ana Peiro Ibanez, Israel Perez Munoz, Ganapathy R Perianayagam, Michael M Petersen, Joris Ploegmakers, Robin Pollock, Gerard Powell, Juan Pretell, Jan Puetzler, Faisal Qamar, Anand Raja, Raja B Rajasekaran, Dipak Ramkumar, R L Randall, Kenneth Rankin, Kevin A Raskin, Kumaran Rassppan, Lauris Repsa, Mickael Ropars, Peter Rose, Pietro Ruggieri, Wael Sadek, German Salcedo, Aasim Saleemi, Andrea Sambri, Hartej Sar, Roberto Scanferla, Thomas Schubert, Jan Schwarze, Guido Scoccianti, Ashley Scrimshire, Tetsuya Sekita, Ahmad Shehadeh, Ahmed Shoaib, Bhim Shreemal, Felix Shumelinsky, Geoffrey Siegel, Claudio Silveri, Robert Silverwood, Friedl Sinnaeve, Jerome Sison, Andrea Slade, Maria A Smolle, Franz Snyman, Scott Sommerville, Sahil Sood, Andre Spiguel, Hugo St-Yves, Eric L Staals, Silvia Stacchiotti, Nikolas Stavropoulos, Peter Steadman, Jonathan D Stevenson, Mikaela Sullivan, Gwen Sys, Bartlomiej Szostakowski, Angela Tamburini, Yuta Taniguchi, Thomas Temple, Christoph Theil, Joachim Thorkildsen, Meagan Tibbo, Roger Tillman, Yu Toda, Kaspar Tootsi, Ferran Torner-Rubies, Frank Traub, Ioannis Trikoupis, Panagiotis Tsagkozis, Kim Tsoi, Hiroyuki Tsuchiya, Veli-Matti Vainio, Antonio Valcarcel, Juan Valencia, Annelies Van Beeck, Michiel Van De Sande, Thomas Van Den Berghe, Ingrid Van Der Geest, Lizz Van Der Heijden, Robert Van Der Wal, Kirsten Van Langevelde, Gualter Vaz, Roberto Velez Villa, Floortje Verspoor, Koenraad Verstraete, Julia Visgauss, Oleg Vyrva, Hazem Wafa, Sebastian Walter, Wan F Wan Ismail, Edward Wang, Patrick Q Wang, David Warnock, Joel Werier, Wolfram Weschenfelder, Kwok-Chuen Wong, Marjan Wouthuyzen-Bakker, Jay Wunder, Indica Wysinghe, Norio Yamamoto, Zhaoming Ye, Seung-Jae Yoon, Suraya Zainul Abidin, Pierluca Zecchetto, Liuzhe Zhang, Juan P Zumarraga, Miguel A Clara-Altamirano

Faculty, Staff and Student Publications

Aims: Cancer care guidelines have been developed in many subspecialities, usually in advanced health systems. However, there are notable global disparities in healthcare access, which can impact sarcoma care. Unfortunately, there is a lack of global data on this subject. Our aim was to describe access to sarcoma care based on a comprehensive global survey among orthopaedic oncologists, and assess for global as well as regional differences.

Methods: A 25-question survey was emailed to the attendees of the 2024 Birmingham Orthopaedic Oncology Meeting and included questions about the respondents' training and practice, access to sarcoma centres, and specific items for …

Natural Killer Cells’ Functional Impairment Drives The Immune Escape Of Pre-Malignant Clones In Early-Stage Myelodysplastic Syndromes, Juan Jose Rodriguez-Sevilla, Irene Ganan-Gomez, Bijender Kumar, Natthakan Thongon, Feiyang Ma, Kelly S Chien, Yi J Kim, Hui Yang, Sanam Loghavi, Roselyn Tan, Vera Adema, Zongrui Li, Tomoyuki Tanaka, Hidetaka Uryu, Rashmi Kanagal-Shamanna, Gheath Al-Atrash, Rafael Bejar, Pinaki Prosad Banerjee, Sophia Lynn Cha, Guillermo Montalban-Bravo, Max Dougherty, Maria Claudina Fernandez Laurita, Noelle Wheeler, Baosen Jia, Eirini P Papapetrou, Franco Izzo, Daniela E Dueñas, Salome Mcallen, Yiqian Gu, Gabriele Todisco, Francesca Ficara, Matteo Giovanni Della Porta, Abhinav Jain, Koichi Takahashi, Karen Clise-Dwyer, Stephanie Halene, Maria Teresa Sabrina Bertilaccio, Guillermo Garcia-Manero, May Daher, Simona Colla

Faculty, Staff and Student Publications

Dissecting the preneoplastic disease states' biological mechanisms that precede tumorigenesis can lead to interventions that can slow down disease progression and/or mitigate disease-related comorbidities. Myelodysplastic syndromes (MDS) cannot be cured by currently available pharmacological therapies, which fail to eradicate aberrant hematopoietic stem cells (HSCs), most of which are mutated by the time of diagnosis. Here, we sought to elucidate how MDS HSCs evade immune surveillance and expand in patients with clonal cytopenias of undetermined significance (CCUS), the pre-malignant stage of MDS. We used multi-omic single-cell approaches and functional in vitro studies to show that immune escape at disease initiation is …

Impacts Of Riverine Heatwaves On Rates Of Ecosystem Metabolism In The United States, Spencer J. Tassone, Michelle Kelly, Olivia N. Beidler, Michael L. Pace, Amy Marcarelli

Michigan Tech Publications

Rivers produce and decompose large amounts of carbon globally due, in part, to high rates of gross primary production (GPP) and ecosystem respiration (ER), collectively known as ecosystem metabolism. Water temperature is a major driver of ecosystem metabolism, and in-stream temperatures are increasing globally, including extreme temperature events called heatwaves. This study used published estimates of daily GPP and ER from 48 stream and river locations in the United States to examine how ecosystem metabolism responds to riverine heatwaves. During low-severity heatwaves, GPP and ER increase proportionally, resulting in no net difference. However, during severe and extreme heatwaves, GPP declined …

Advantages, Disadvantages, And Factors Influencing The Reproductive Performance Of Gilts And Sows Raised In Gestational Group Housing And Free-Farrowing Systems, Natchanon Dumniem Dr., Thomas D. Parsons, Padet Tummaruk

The Thai Journal of Veterinary Medicine

In the modern global pig industry, there is a growing interest in animal-friendly agriculture, encouraging farmers to prioritize humane practices and meet the welfare needs of their animals. In developed countries, consumers are increasingly concerned about animal welfare, particularly regarding pigs as social animals, advocating for their housing in groups that reflect their natural behaviors in the wild. There is significant societal pressure to eliminate the use of crate systems, which cause discomfort to animals in commercial farming. In these systems, breeding females are often restricted in movement throughout the reproductive cycle. As a result, alternative husbandry and housing systems …

Rapid Quantification Of Salmonella Typhimurium In Ground Chicken Using Immunomagnetic Chemiluminescent Assay, Sandhya Thapa, Niraj Ghimire, Fur-Chi Chen

Food and Animal Sciences Faculty Research

Many countries have established regulatory frameworks to monitor and mitigate Salmonella contamination in poultry products. The ability to rapidly quantify Salmonella is critical for poultry processors to facilitate early detection, implement corrective measures, and enhance product safety. This study aimed to develop an Immunomagnetic Chemiluminescent Assay (IMCA) for the quantification of Salmonella Typhimurium in ground chicken. Immunomagnetic microbeads functionalized with monoclonal antibodies were employed to selectively capture and concentrate Salmonella from ground chicken samples. A biotin-labeled monoclonal antibody, followed by an avidin-horseradish peroxidase conjugate, was used to bind the captured bacteria and initiate a chemiluminescent reaction catalyzed by peroxidase. Light …

The Impact Of Diet-Induced Obesity On Cerebrovasculature Structure And Function, Riley M. Feagle

MUSC Theses and Dissertations

Adequate cerebral perfusion is necessary to help the brain maintain a constant and steady state of nutrient delivery. Since the brain is highly metabolic, changes in the vasculature structure can impact perfusion levels and, therefore, reduce the ability of the brain to receive the support necessary for full functionality. Vascular dysfunction is a common comorbidity among obese individuals. Given the strong association between obesity and cognitive dysfunction, understanding the correlation between reduced cerebral perfusion and altered cerebrovascular structure is crucial. The overall hypothesis of this thesis originated after ample preliminary data, and states that diet-induced obesity reduces cerebral perfusion and …

The Zbtb24-Cdca7-Hells Axis Suppresses The Totipotent 2c-Like Reprogramming By Maintaining Dux Methylation And Repression, Dan Guo, Zeling Du, Youqi Liu, Meiqi Lin, Yue Lu, Swanand Hardikar, Yanna Xue, Jinghong Zhang, Taiping Chen, Jiameng Dan

Faculty, Staff and Student Publications

Two-cell-like cells (2CLCs), a rare population (∼0.5%) in mouse embryonic stem cell (mESC) cultures, are in a transient totipotent-like state resembling that of 2C-stage embryos, and their discovery and characterization have greatly facilitated the study of early developmental events, such as zygotic genome activation. However, the molecular determinants governing 2C-like reprogramming remain to be elucidated. Here, we show that ZBTB24, CDCA7, and HELLS, components of a molecular pathway that is involved in the pathogenesis of immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, function as negative regulators of 2C-like reprogramming by maintaining DNA methylation of the Dux cluster, a master …

Allosteric Targeted Drug Delivery For Enhanced Blood-Brain Barrier Penetration Via Mimicking Transmembrane Domain Interactions, Kaicheng Tang, Zhongjie Tang, Miaomiao Niu, Zuyin Kuang, Weiwei Xue, Xinyu Wang, Xinlong Liu, Yang Yu, Seongdong Jeong, Yifan Ma, Annette Wu, Betty Y S Kim, Wen Jiang, Zhaogang Yang, Chong Li

Faculty, Staff and Student Publications

Current strategies for active targeting in the brain are entirely based on the effective interaction of the ligand with the orthosteric sites of specific receptors on the blood-brain barrier (BBB), which is highly susceptible to various pathophysiological factors and limits the efficacy of drug delivery. Here, we propose an allosteric targeted drug delivery strategy that targets classical BBB transmembrane receptors by designing peptide ligands that specifically bind to their transmembrane domains. This strategy prevents competitive interference from endogenous ligands and antibodies by using the insulin receptor and integrin α

Crop Advisor Approaches To Soil Health Advising And Opportunities For Improvement, Peggy Petrzelka, Jessica D. Ulrich-Schad, Matt Yost, Jenae Dean, Earl Creech, Linda Schott, Tony Richards, Kristy Davis

All Current Publications

In this report, we use data from a 2023 online survey of crop advisors in Utah and Idaho to examine how they are working with their clients on soil health and where opportunities for improvement exist.

Cognitive Correlates Of Borderline Personality Disorder Features In Youth With Bipolar Spectrum Disorders And Bipolar Offspring, Alessio Simonetti, Sherin Kurian, Evelina Bernardi, Antonio Restaino, Francesca Bardi, Claudia Calderoni, Gabriele Sani, Jair C Soares, Kirti Saxena

Faculty, Staff and Student Publications

Background: Bipolar disorder (BD) and borderline personality disorder (BPD) share common cognitive impairments. These deficits are also shared by bipolar offspring (BD-OFF). Nevertheless, little is known regarding the association between cognitive impairments and BPD features in youth with BD and BD-OFF. Objectives: This study aimed to investigate the association between BPD features and cognitive impairments in youth with BD and BD-OFF.

Methods: Thirty-nine participants (7-17 years) with BD, 18 BD-OFF, and 50 healthy controls (HCs) were recruited. BPD features were assessed using the Borderline Personality Features Scale for Children (BPFS-C). Deficits in executive functions and affective processing were assessed using …

Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer Mullen

Student Research Symposium

Introduction

Animal models are key to the research and development of antiviral interventions. Two strains of low-pathogenic avian influenza virus, A/Anhui/1/2013 and A/Shanghai/1/2013 (H7N9), isolated from a 2013 outbreak in China and acquired by USU’s Institute for Antiviral Research from the CDC, are the subject of this project.

Developing antiviral interventions against Influenza A H7N9 is essential because:

• Global outbreaks have caused high mortality among bird and human populations since 2013.^[1]

• Low-pathogenic avian flu viruses can mutate to become highly pathogenic.^[2]

• No current prophylactic or therapeutic human interventions exist.^[3]

BALB/c mice, commonly used for …

Evaluating The Stress System Of The Grieving Brain: Corticotropin-Releasing Factors And Partner Loss In Coyotes, Rachel Tong

Student Research Symposium

Introduction

Social bonds are fundamental and essential

• Positive and negative effects

The stress system¹ :

• Corticotropin-releasing factor (CRF) system
• CRFR1 and CRFR2
• Acts on the HPA axis, amygdala, and hippocampus

Genetic monogamy is common among coyotes

Importance:

• Coyotes’ greatest threat are humans
• An abandoned partner can disrupt biology and behavior

Animals experience social loss too!

Biosecurity Strategies For Combating Iuu Fishing: Protecting Ecosystems And Economies, Spencer Anderson

Student Research Symposium

Illegal, Unreported, and Unregulated (IUU) fishing threatens marine ecosystems and global economies by driving overfishing, spreading aquatic diseases, and introducing invasive species. It disrupts biodiversity, jeopardizes fisheries' sustainability, and undermines the livelihoods of millions, intensifying food insecurity and socio- economic vulnerabilities, particularly in fishing- dependent communities.

This research examines biosecurity strategies— including regulatory frameworks and collaborative efforts—to safeguard marine biodiversity and support sustainable fishing economies. Addressing the complex challenges of IUU fishing bridges gaps between conservation, public health, and economic resilience.

This study adopts an interdisciplinary approach to develop practical solutions to preserve biodiversity, enhance public health protections, and promote …

Establishing Methods For In Vitro Static Culture Of Gut Microbiomes For Nutritional Intervention Studies, Jeremy Case

Student Research Symposium

The National Cancer Institute reports colorectal cancer as the leading cause of cancer death for individuals under 50 in the United States (American Cancer Society). These rates continue to rise with poor diet, microbiome dysbiosis, and gut inflammation being the primary drivers.

Our lab performs nutrient- intervention studies on DSS/AOM colitis-induced mouse models fed the total Western diet, reflective of the average American’s diet. A poor diet promotes gut microbiome dysbiosis and subsequent colitis, ultimately yielding colon cancer. We seek to understand how the introduction of a bioactive compound may lessen or promote these effects in in vivo mouse models. …

Exercise And Its Effect On The Nad Content Of Tissues In Niacin Deficient Mice With Free Choice Running Wheels, Stellaluna Star

Student Research Symposium

Nicotinamide adenine dinucleotide (NAD) content of tissues decreases over time during aging. In our study, we determine if physical exercise has an effect on the NAD content in tissues of transgenic mice that were NAD- deficient because of vitamin B3-free diet. The underlying hypothesis of this study is that exercise can prevent NAD decline and maintain higher level of NAD in tissue. A potential impact of this study is that exercise could be used to lengthen healthspan compared to individuals not participating in any physical exercise.

Grassland For Our World, F Riveros

IGC Proceedings (1977-2023)

The present-day role of grasslands and fodder is reviewed with respect agriculture, animal production and environmental concerns. The world 's grazing lands are a finite resource nnd, overall, are being badly mnnaEed; in most developing countries action is required now, before it is too lnte. They will continue to be a major source of animal feed for the foreseeable future; encroachment from cropping will con

Effect Of Irrigation On Vegetation Succession And Forage Regerneration, Fu Jing-Yan

IGC Proceedings (1977-2023)

No abstract provided.